De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing

Abstract Pearl millet (Pennisetum glaucum L.) is one of the most important small-grained annual cereal crops grown in the arid and semi-arid regions of India and Africa, where terminal drought is a major constraint for its productivity. Terminal drought stress is more damaging to pearl millet than d...
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Autor*in:	Shivhare, Radha [verfasserIn] Lakhwani, Deepika [verfasserIn] Asif, Mehar H. [verfasserIn] Chauhan, Puneet S. [verfasserIn] Lata, Charu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	Comparative transcriptome De novo assembly Pearl millet Terminal drought

Übergeordnetes Werk:	Enthalten in: The nucleus - [New Delhi] : Springer India, 2010, 63(2020), 3 vom: 20. Juni, Seite 341-352
Übergeordnetes Werk:	volume:63 ; year:2020 ; number:3 ; day:20 ; month:06 ; pages:341-352

Links:	Volltext

DOI / URN:	10.1007/s13237-020-00324-1

Katalog-ID:	SPR042178746

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245	1	3	\|a De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing
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520			\|a Abstract Pearl millet (Pennisetum glaucum L.) is one of the most important small-grained annual cereal crops grown in the arid and semi-arid regions of India and Africa, where terminal drought is a major constraint for its productivity. Terminal drought stress is more damaging to pearl millet than drought at vegetative stage as 50% or more reduction in yield may incur. However, a pearl millet genotype, PRLT2/89-33, parent of a mapping population is tolerant to terminal drought stress. With the aim to understand the molecular mechanism underlying terminal drought tolerance in this naturally drought tolerant crop, we examined the leaf transcriptome of contrasting pearl millet genotypes namely, PRLT2/89-33 and H77/833-2 differing for terminal drought tolerance using illumina Miseq sequencing platform. In this study, 40,880 genes were found to be differentially expressed and 8273 unigenes showed significant similarities to known sequences in NCBI non-redundant (nr) database and were classified into 189 Gene Ontology and 24 Clusters of Orthologous Group terms for both the genotypes. Expression profiling of 13 randomly selected transcripts was done to validate the RNA-Seq data. The study revealed that the genes for phytohormones biosynthesis, secondary metabolites and abiotic stress related transcription factors were more expressed in PRLT2/89-33, throwing insights into the molecular basis of its terminal drought tolerance. The study also supported that Illumina Miseq Platform is a powerful tool for transcriptome analysis and molecular-marker development in economically important non-model crop species, particularly those with large and complex genomes.
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author_variant	r s rs d l dl m h a mh mha p s c ps psc c l cl
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allfields	10.1007/s13237-020-00324-1 doi (DE-627)SPR042178746 (DE-599)SPRs13237-020-00324-1-e (SPR)s13237-020-00324-1-e DE-627 ger DE-627 rakwb eng 610 ASE Shivhare, Radha verfasserin aut De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Pearl millet (Pennisetum glaucum L.) is one of the most important small-grained annual cereal crops grown in the arid and semi-arid regions of India and Africa, where terminal drought is a major constraint for its productivity. Terminal drought stress is more damaging to pearl millet than drought at vegetative stage as 50% or more reduction in yield may incur. However, a pearl millet genotype, PRLT2/89-33, parent of a mapping population is tolerant to terminal drought stress. With the aim to understand the molecular mechanism underlying terminal drought tolerance in this naturally drought tolerant crop, we examined the leaf transcriptome of contrasting pearl millet genotypes namely, PRLT2/89-33 and H77/833-2 differing for terminal drought tolerance using illumina Miseq sequencing platform. In this study, 40,880 genes were found to be differentially expressed and 8273 unigenes showed significant similarities to known sequences in NCBI non-redundant (nr) database and were classified into 189 Gene Ontology and 24 Clusters of Orthologous Group terms for both the genotypes. Expression profiling of 13 randomly selected transcripts was done to validate the RNA-Seq data. The study revealed that the genes for phytohormones biosynthesis, secondary metabolites and abiotic stress related transcription factors were more expressed in PRLT2/89-33, throwing insights into the molecular basis of its terminal drought tolerance. The study also supported that Illumina Miseq Platform is a powerful tool for transcriptome analysis and molecular-marker development in economically important non-model crop species, particularly those with large and complex genomes. Comparative transcriptome (dpeaa)DE-He213 De novo assembly (dpeaa)DE-He213 Pearl millet (dpeaa)DE-He213 Terminal drought (dpeaa)DE-He213 Lakhwani, Deepika verfasserin aut Asif, Mehar H. verfasserin aut Chauhan, Puneet S. verfasserin aut Lata, Charu verfasserin aut Enthalten in The nucleus [New Delhi] : Springer India, 2010 63(2020), 3 vom: 20. Juni, Seite 341-352 (DE-627)644282746 (DE-600)2589081-5 0976-7975 nnns volume:63 year:2020 number:3 day:20 month:06 pages:341-352 https://dx.doi.org/10.1007/s13237-020-00324-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 63 2020 3 20 06 341-352
spelling	10.1007/s13237-020-00324-1 doi (DE-627)SPR042178746 (DE-599)SPRs13237-020-00324-1-e (SPR)s13237-020-00324-1-e DE-627 ger DE-627 rakwb eng 610 ASE Shivhare, Radha verfasserin aut De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Pearl millet (Pennisetum glaucum L.) is one of the most important small-grained annual cereal crops grown in the arid and semi-arid regions of India and Africa, where terminal drought is a major constraint for its productivity. Terminal drought stress is more damaging to pearl millet than drought at vegetative stage as 50% or more reduction in yield may incur. However, a pearl millet genotype, PRLT2/89-33, parent of a mapping population is tolerant to terminal drought stress. With the aim to understand the molecular mechanism underlying terminal drought tolerance in this naturally drought tolerant crop, we examined the leaf transcriptome of contrasting pearl millet genotypes namely, PRLT2/89-33 and H77/833-2 differing for terminal drought tolerance using illumina Miseq sequencing platform. In this study, 40,880 genes were found to be differentially expressed and 8273 unigenes showed significant similarities to known sequences in NCBI non-redundant (nr) database and were classified into 189 Gene Ontology and 24 Clusters of Orthologous Group terms for both the genotypes. Expression profiling of 13 randomly selected transcripts was done to validate the RNA-Seq data. The study revealed that the genes for phytohormones biosynthesis, secondary metabolites and abiotic stress related transcription factors were more expressed in PRLT2/89-33, throwing insights into the molecular basis of its terminal drought tolerance. The study also supported that Illumina Miseq Platform is a powerful tool for transcriptome analysis and molecular-marker development in economically important non-model crop species, particularly those with large and complex genomes. Comparative transcriptome (dpeaa)DE-He213 De novo assembly (dpeaa)DE-He213 Pearl millet (dpeaa)DE-He213 Terminal drought (dpeaa)DE-He213 Lakhwani, Deepika verfasserin aut Asif, Mehar H. verfasserin aut Chauhan, Puneet S. verfasserin aut Lata, Charu verfasserin aut Enthalten in The nucleus [New Delhi] : Springer India, 2010 63(2020), 3 vom: 20. Juni, Seite 341-352 (DE-627)644282746 (DE-600)2589081-5 0976-7975 nnns volume:63 year:2020 number:3 day:20 month:06 pages:341-352 https://dx.doi.org/10.1007/s13237-020-00324-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 63 2020 3 20 06 341-352
allfields_unstemmed	10.1007/s13237-020-00324-1 doi (DE-627)SPR042178746 (DE-599)SPRs13237-020-00324-1-e (SPR)s13237-020-00324-1-e DE-627 ger DE-627 rakwb eng 610 ASE Shivhare, Radha verfasserin aut De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Pearl millet (Pennisetum glaucum L.) is one of the most important small-grained annual cereal crops grown in the arid and semi-arid regions of India and Africa, where terminal drought is a major constraint for its productivity. Terminal drought stress is more damaging to pearl millet than drought at vegetative stage as 50% or more reduction in yield may incur. However, a pearl millet genotype, PRLT2/89-33, parent of a mapping population is tolerant to terminal drought stress. With the aim to understand the molecular mechanism underlying terminal drought tolerance in this naturally drought tolerant crop, we examined the leaf transcriptome of contrasting pearl millet genotypes namely, PRLT2/89-33 and H77/833-2 differing for terminal drought tolerance using illumina Miseq sequencing platform. In this study, 40,880 genes were found to be differentially expressed and 8273 unigenes showed significant similarities to known sequences in NCBI non-redundant (nr) database and were classified into 189 Gene Ontology and 24 Clusters of Orthologous Group terms for both the genotypes. Expression profiling of 13 randomly selected transcripts was done to validate the RNA-Seq data. The study revealed that the genes for phytohormones biosynthesis, secondary metabolites and abiotic stress related transcription factors were more expressed in PRLT2/89-33, throwing insights into the molecular basis of its terminal drought tolerance. The study also supported that Illumina Miseq Platform is a powerful tool for transcriptome analysis and molecular-marker development in economically important non-model crop species, particularly those with large and complex genomes. Comparative transcriptome (dpeaa)DE-He213 De novo assembly (dpeaa)DE-He213 Pearl millet (dpeaa)DE-He213 Terminal drought (dpeaa)DE-He213 Lakhwani, Deepika verfasserin aut Asif, Mehar H. verfasserin aut Chauhan, Puneet S. verfasserin aut Lata, Charu verfasserin aut Enthalten in The nucleus [New Delhi] : Springer India, 2010 63(2020), 3 vom: 20. Juni, Seite 341-352 (DE-627)644282746 (DE-600)2589081-5 0976-7975 nnns volume:63 year:2020 number:3 day:20 month:06 pages:341-352 https://dx.doi.org/10.1007/s13237-020-00324-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 63 2020 3 20 06 341-352
allfieldsGer	10.1007/s13237-020-00324-1 doi (DE-627)SPR042178746 (DE-599)SPRs13237-020-00324-1-e (SPR)s13237-020-00324-1-e DE-627 ger DE-627 rakwb eng 610 ASE Shivhare, Radha verfasserin aut De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Pearl millet (Pennisetum glaucum L.) is one of the most important small-grained annual cereal crops grown in the arid and semi-arid regions of India and Africa, where terminal drought is a major constraint for its productivity. Terminal drought stress is more damaging to pearl millet than drought at vegetative stage as 50% or more reduction in yield may incur. However, a pearl millet genotype, PRLT2/89-33, parent of a mapping population is tolerant to terminal drought stress. With the aim to understand the molecular mechanism underlying terminal drought tolerance in this naturally drought tolerant crop, we examined the leaf transcriptome of contrasting pearl millet genotypes namely, PRLT2/89-33 and H77/833-2 differing for terminal drought tolerance using illumina Miseq sequencing platform. In this study, 40,880 genes were found to be differentially expressed and 8273 unigenes showed significant similarities to known sequences in NCBI non-redundant (nr) database and were classified into 189 Gene Ontology and 24 Clusters of Orthologous Group terms for both the genotypes. Expression profiling of 13 randomly selected transcripts was done to validate the RNA-Seq data. The study revealed that the genes for phytohormones biosynthesis, secondary metabolites and abiotic stress related transcription factors were more expressed in PRLT2/89-33, throwing insights into the molecular basis of its terminal drought tolerance. The study also supported that Illumina Miseq Platform is a powerful tool for transcriptome analysis and molecular-marker development in economically important non-model crop species, particularly those with large and complex genomes. Comparative transcriptome (dpeaa)DE-He213 De novo assembly (dpeaa)DE-He213 Pearl millet (dpeaa)DE-He213 Terminal drought (dpeaa)DE-He213 Lakhwani, Deepika verfasserin aut Asif, Mehar H. verfasserin aut Chauhan, Puneet S. verfasserin aut Lata, Charu verfasserin aut Enthalten in The nucleus [New Delhi] : Springer India, 2010 63(2020), 3 vom: 20. Juni, Seite 341-352 (DE-627)644282746 (DE-600)2589081-5 0976-7975 nnns volume:63 year:2020 number:3 day:20 month:06 pages:341-352 https://dx.doi.org/10.1007/s13237-020-00324-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 63 2020 3 20 06 341-352
allfieldsSound	10.1007/s13237-020-00324-1 doi (DE-627)SPR042178746 (DE-599)SPRs13237-020-00324-1-e (SPR)s13237-020-00324-1-e DE-627 ger DE-627 rakwb eng 610 ASE Shivhare, Radha verfasserin aut De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Pearl millet (Pennisetum glaucum L.) is one of the most important small-grained annual cereal crops grown in the arid and semi-arid regions of India and Africa, where terminal drought is a major constraint for its productivity. Terminal drought stress is more damaging to pearl millet than drought at vegetative stage as 50% or more reduction in yield may incur. However, a pearl millet genotype, PRLT2/89-33, parent of a mapping population is tolerant to terminal drought stress. With the aim to understand the molecular mechanism underlying terminal drought tolerance in this naturally drought tolerant crop, we examined the leaf transcriptome of contrasting pearl millet genotypes namely, PRLT2/89-33 and H77/833-2 differing for terminal drought tolerance using illumina Miseq sequencing platform. In this study, 40,880 genes were found to be differentially expressed and 8273 unigenes showed significant similarities to known sequences in NCBI non-redundant (nr) database and were classified into 189 Gene Ontology and 24 Clusters of Orthologous Group terms for both the genotypes. Expression profiling of 13 randomly selected transcripts was done to validate the RNA-Seq data. The study revealed that the genes for phytohormones biosynthesis, secondary metabolites and abiotic stress related transcription factors were more expressed in PRLT2/89-33, throwing insights into the molecular basis of its terminal drought tolerance. The study also supported that Illumina Miseq Platform is a powerful tool for transcriptome analysis and molecular-marker development in economically important non-model crop species, particularly those with large and complex genomes. Comparative transcriptome (dpeaa)DE-He213 De novo assembly (dpeaa)DE-He213 Pearl millet (dpeaa)DE-He213 Terminal drought (dpeaa)DE-He213 Lakhwani, Deepika verfasserin aut Asif, Mehar H. verfasserin aut Chauhan, Puneet S. verfasserin aut Lata, Charu verfasserin aut Enthalten in The nucleus [New Delhi] : Springer India, 2010 63(2020), 3 vom: 20. Juni, Seite 341-352 (DE-627)644282746 (DE-600)2589081-5 0976-7975 nnns volume:63 year:2020 number:3 day:20 month:06 pages:341-352 https://dx.doi.org/10.1007/s13237-020-00324-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 63 2020 3 20 06 341-352
language	English
source	Enthalten in The nucleus 63(2020), 3 vom: 20. Juni, Seite 341-352 volume:63 year:2020 number:3 day:20 month:06 pages:341-352
sourceStr	Enthalten in The nucleus 63(2020), 3 vom: 20. Juni, Seite 341-352 volume:63 year:2020 number:3 day:20 month:06 pages:341-352
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Comparative transcriptome De novo assembly Pearl millet Terminal drought
dewey-raw	610
isfreeaccess_bool	false
container_title	The nucleus
authorswithroles_txt_mv	Shivhare, Radha @@aut@@ Lakhwani, Deepika @@aut@@ Asif, Mehar H. @@aut@@ Chauhan, Puneet S. @@aut@@ Lata, Charu @@aut@@
publishDateDaySort_date	2020-06-20T00:00:00Z
hierarchy_top_id	644282746
dewey-sort	3610
id	SPR042178746
language_de	englisch
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author	Shivhare, Radha
spellingShingle	Shivhare, Radha ddc 610 misc Comparative transcriptome misc De novo assembly misc Pearl millet misc Terminal drought De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing
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topic_title	610 ASE De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing Comparative transcriptome (dpeaa)DE-He213 De novo assembly (dpeaa)DE-He213 Pearl millet (dpeaa)DE-He213 Terminal drought (dpeaa)DE-He213
topic	ddc 610 misc Comparative transcriptome misc De novo assembly misc Pearl millet misc Terminal drought
topic_unstemmed	ddc 610 misc Comparative transcriptome misc De novo assembly misc Pearl millet misc Terminal drought
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title	De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing
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title_full	De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing
author_sort	Shivhare, Radha
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author_browse	Shivhare, Radha Lakhwani, Deepika Asif, Mehar H. Chauhan, Puneet S. Lata, Charu
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class	610 ASE
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author-letter	Shivhare, Radha
doi_str_mv	10.1007/s13237-020-00324-1
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title_sort	novo assembly and comparative transcriptome analysis of contrasting pearl millet (pennisetum glaucum l.) genotypes under terminal drought stress using illumina sequencing
title_auth	De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing
abstract	Abstract Pearl millet (Pennisetum glaucum L.) is one of the most important small-grained annual cereal crops grown in the arid and semi-arid regions of India and Africa, where terminal drought is a major constraint for its productivity. Terminal drought stress is more damaging to pearl millet than drought at vegetative stage as 50% or more reduction in yield may incur. However, a pearl millet genotype, PRLT2/89-33, parent of a mapping population is tolerant to terminal drought stress. With the aim to understand the molecular mechanism underlying terminal drought tolerance in this naturally drought tolerant crop, we examined the leaf transcriptome of contrasting pearl millet genotypes namely, PRLT2/89-33 and H77/833-2 differing for terminal drought tolerance using illumina Miseq sequencing platform. In this study, 40,880 genes were found to be differentially expressed and 8273 unigenes showed significant similarities to known sequences in NCBI non-redundant (nr) database and were classified into 189 Gene Ontology and 24 Clusters of Orthologous Group terms for both the genotypes. Expression profiling of 13 randomly selected transcripts was done to validate the RNA-Seq data. The study revealed that the genes for phytohormones biosynthesis, secondary metabolites and abiotic stress related transcription factors were more expressed in PRLT2/89-33, throwing insights into the molecular basis of its terminal drought tolerance. The study also supported that Illumina Miseq Platform is a powerful tool for transcriptome analysis and molecular-marker development in economically important non-model crop species, particularly those with large and complex genomes.
abstractGer	Abstract Pearl millet (Pennisetum glaucum L.) is one of the most important small-grained annual cereal crops grown in the arid and semi-arid regions of India and Africa, where terminal drought is a major constraint for its productivity. Terminal drought stress is more damaging to pearl millet than drought at vegetative stage as 50% or more reduction in yield may incur. However, a pearl millet genotype, PRLT2/89-33, parent of a mapping population is tolerant to terminal drought stress. With the aim to understand the molecular mechanism underlying terminal drought tolerance in this naturally drought tolerant crop, we examined the leaf transcriptome of contrasting pearl millet genotypes namely, PRLT2/89-33 and H77/833-2 differing for terminal drought tolerance using illumina Miseq sequencing platform. In this study, 40,880 genes were found to be differentially expressed and 8273 unigenes showed significant similarities to known sequences in NCBI non-redundant (nr) database and were classified into 189 Gene Ontology and 24 Clusters of Orthologous Group terms for both the genotypes. Expression profiling of 13 randomly selected transcripts was done to validate the RNA-Seq data. The study revealed that the genes for phytohormones biosynthesis, secondary metabolites and abiotic stress related transcription factors were more expressed in PRLT2/89-33, throwing insights into the molecular basis of its terminal drought tolerance. The study also supported that Illumina Miseq Platform is a powerful tool for transcriptome analysis and molecular-marker development in economically important non-model crop species, particularly those with large and complex genomes.
abstract_unstemmed	Abstract Pearl millet (Pennisetum glaucum L.) is one of the most important small-grained annual cereal crops grown in the arid and semi-arid regions of India and Africa, where terminal drought is a major constraint for its productivity. Terminal drought stress is more damaging to pearl millet than drought at vegetative stage as 50% or more reduction in yield may incur. However, a pearl millet genotype, PRLT2/89-33, parent of a mapping population is tolerant to terminal drought stress. With the aim to understand the molecular mechanism underlying terminal drought tolerance in this naturally drought tolerant crop, we examined the leaf transcriptome of contrasting pearl millet genotypes namely, PRLT2/89-33 and H77/833-2 differing for terminal drought tolerance using illumina Miseq sequencing platform. In this study, 40,880 genes were found to be differentially expressed and 8273 unigenes showed significant similarities to known sequences in NCBI non-redundant (nr) database and were classified into 189 Gene Ontology and 24 Clusters of Orthologous Group terms for both the genotypes. Expression profiling of 13 randomly selected transcripts was done to validate the RNA-Seq data. The study revealed that the genes for phytohormones biosynthesis, secondary metabolites and abiotic stress related transcription factors were more expressed in PRLT2/89-33, throwing insights into the molecular basis of its terminal drought tolerance. The study also supported that Illumina Miseq Platform is a powerful tool for transcriptome analysis and molecular-marker development in economically important non-model crop species, particularly those with large and complex genomes.
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title_short	De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing
url	https://dx.doi.org/10.1007/s13237-020-00324-1
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author2	Lakhwani, Deepika Asif, Mehar H. Chauhan, Puneet S. Lata, Charu
author2Str	Lakhwani, Deepika Asif, Mehar H. Chauhan, Puneet S. Lata, Charu
ppnlink	644282746
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doi_str	10.1007/s13237-020-00324-1
up_date	2024-07-04T01:09:36.751Z
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Terminal drought stress is more damaging to pearl millet than drought at vegetative stage as 50% or more reduction in yield may incur. However, a pearl millet genotype, PRLT2/89-33, parent of a mapping population is tolerant to terminal drought stress. With the aim to understand the molecular mechanism underlying terminal drought tolerance in this naturally drought tolerant crop, we examined the leaf transcriptome of contrasting pearl millet genotypes namely, PRLT2/89-33 and H77/833-2 differing for terminal drought tolerance using illumina Miseq sequencing platform. In this study, 40,880 genes were found to be differentially expressed and 8273 unigenes showed significant similarities to known sequences in NCBI non-redundant (nr) database and were classified into 189 Gene Ontology and 24 Clusters of Orthologous Group terms for both the genotypes. Expression profiling of 13 randomly selected transcripts was done to validate the RNA-Seq data. 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De novo assembly and comparative transcriptome analysis of contrasting pearl millet (Pennisetum glaucum L.) genotypes under terminal drought stress using illumina sequencing

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