Electrically Tunable Ultra-specific Zinc Oxide Biosensor
Abstract Zinc oxide surface states can be utilized for ultra-specific detection of biomolecules. The major challenges in using ZnO for bio-sensing are attaining enhanced sensitivity and specificity. In this study, we explore the functionalization of zinc in ZnO through utilizing the thiol bond. The...
Ausführliche Beschreibung
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| Autor*in: |
Munje, Rujuta D. [verfasserIn] Wangzhou, Andi [verfasserIn] Selvam, Anjan Panneer [verfasserIn] Muthukumar, Sriram [verfasserIn] Prasad, Shalini [verfasserIn] |
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E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014 |
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Enthalten in: MRS online proceedings library - Warrendale, Pa. : MRS, 1998, 1720(2014), 1 vom: Dez., Seite 33-38 |
|---|---|
| Übergeordnetes Werk: |
volume:1720 ; year:2014 ; number:1 ; month:12 ; pages:33-38 |
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| DOI / URN: |
10.1557/opl.2015.62 |
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| 520 | |a Abstract Zinc oxide surface states can be utilized for ultra-specific detection of biomolecules. The major challenges in using ZnO for bio-sensing are attaining enhanced sensitivity and specificity. In this study, we explore the functionalization of zinc in ZnO through utilizing the thiol bond. The purpose of this study is to demonstrate that the ZnO based sensor is capable of achieving high specificity in presence of competitive surface binding through the thiol bond. The final goal is to design an ultra-specific biosensor to detect low occurring biomolecules. In this study, we have selected cortisol as a stress marker to demonstrate quantification and detection from synthetic sweat. In order to demonstrate ultra-specificity, we have used two competitive thiol based molecules binding to zinc, a linker Dithiobis succinimidyl propionate (DSP) and reducing agent of DSP, Dithiothreitol (DTT). Electrochemical impedance spectroscopy (EIS) is used to quantify the signal obtained through various ratiometric concentrations of DSP and DTT. To validate the EIS study results, inherent fluorescence studies are done by mapping changes in green emission spectrum of ZnO before and after linker functionalization. The optimal combination in terms of highest signal is identified to be of 25mM DTT and 50mM DSP. This is implemented in the experiments performed to calibrate the cortisol concentration in synthetic sweat. This study demonstrates the detection of cortisol antigen in synthetic sweat present within the physiological levels of 8 ng/mL to 140 ng/mL. | ||
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| 700 | 1 | |a Prasad, Shalini |e verfasserin |4 aut | |
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10.1557/opl.2015.62 doi (DE-627)SPR042299217 (DE-599)SPRopl.2015.62-e (SPR)opl.2015.62-e DE-627 ger DE-627 rakwb eng 670 ASE Munje, Rujuta D. verfasserin aut Electrically Tunable Ultra-specific Zinc Oxide Biosensor 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Zinc oxide surface states can be utilized for ultra-specific detection of biomolecules. The major challenges in using ZnO for bio-sensing are attaining enhanced sensitivity and specificity. In this study, we explore the functionalization of zinc in ZnO through utilizing the thiol bond. The purpose of this study is to demonstrate that the ZnO based sensor is capable of achieving high specificity in presence of competitive surface binding through the thiol bond. The final goal is to design an ultra-specific biosensor to detect low occurring biomolecules. In this study, we have selected cortisol as a stress marker to demonstrate quantification and detection from synthetic sweat. In order to demonstrate ultra-specificity, we have used two competitive thiol based molecules binding to zinc, a linker Dithiobis succinimidyl propionate (DSP) and reducing agent of DSP, Dithiothreitol (DTT). Electrochemical impedance spectroscopy (EIS) is used to quantify the signal obtained through various ratiometric concentrations of DSP and DTT. To validate the EIS study results, inherent fluorescence studies are done by mapping changes in green emission spectrum of ZnO before and after linker functionalization. The optimal combination in terms of highest signal is identified to be of 25mM DTT and 50mM DSP. This is implemented in the experiments performed to calibrate the cortisol concentration in synthetic sweat. This study demonstrates the detection of cortisol antigen in synthetic sweat present within the physiological levels of 8 ng/mL to 140 ng/mL. Wangzhou, Andi verfasserin aut Selvam, Anjan Panneer verfasserin aut Muthukumar, Sriram verfasserin aut Prasad, Shalini verfasserin aut Enthalten in MRS online proceedings library Warrendale, Pa. : MRS, 1998 1720(2014), 1 vom: Dez., Seite 33-38 (DE-627)57782046X (DE-600)2451008-7 1946-4274 nnns volume:1720 year:2014 number:1 month:12 pages:33-38 https://dx.doi.org/10.1557/opl.2015.62 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_2005 AR 1720 2014 1 12 33-38 |
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10.1557/opl.2015.62 doi (DE-627)SPR042299217 (DE-599)SPRopl.2015.62-e (SPR)opl.2015.62-e DE-627 ger DE-627 rakwb eng 670 ASE Munje, Rujuta D. verfasserin aut Electrically Tunable Ultra-specific Zinc Oxide Biosensor 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Zinc oxide surface states can be utilized for ultra-specific detection of biomolecules. The major challenges in using ZnO for bio-sensing are attaining enhanced sensitivity and specificity. In this study, we explore the functionalization of zinc in ZnO through utilizing the thiol bond. The purpose of this study is to demonstrate that the ZnO based sensor is capable of achieving high specificity in presence of competitive surface binding through the thiol bond. The final goal is to design an ultra-specific biosensor to detect low occurring biomolecules. In this study, we have selected cortisol as a stress marker to demonstrate quantification and detection from synthetic sweat. In order to demonstrate ultra-specificity, we have used two competitive thiol based molecules binding to zinc, a linker Dithiobis succinimidyl propionate (DSP) and reducing agent of DSP, Dithiothreitol (DTT). Electrochemical impedance spectroscopy (EIS) is used to quantify the signal obtained through various ratiometric concentrations of DSP and DTT. To validate the EIS study results, inherent fluorescence studies are done by mapping changes in green emission spectrum of ZnO before and after linker functionalization. The optimal combination in terms of highest signal is identified to be of 25mM DTT and 50mM DSP. This is implemented in the experiments performed to calibrate the cortisol concentration in synthetic sweat. This study demonstrates the detection of cortisol antigen in synthetic sweat present within the physiological levels of 8 ng/mL to 140 ng/mL. Wangzhou, Andi verfasserin aut Selvam, Anjan Panneer verfasserin aut Muthukumar, Sriram verfasserin aut Prasad, Shalini verfasserin aut Enthalten in MRS online proceedings library Warrendale, Pa. : MRS, 1998 1720(2014), 1 vom: Dez., Seite 33-38 (DE-627)57782046X (DE-600)2451008-7 1946-4274 nnns volume:1720 year:2014 number:1 month:12 pages:33-38 https://dx.doi.org/10.1557/opl.2015.62 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_2005 AR 1720 2014 1 12 33-38 |
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10.1557/opl.2015.62 doi (DE-627)SPR042299217 (DE-599)SPRopl.2015.62-e (SPR)opl.2015.62-e DE-627 ger DE-627 rakwb eng 670 ASE Munje, Rujuta D. verfasserin aut Electrically Tunable Ultra-specific Zinc Oxide Biosensor 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Zinc oxide surface states can be utilized for ultra-specific detection of biomolecules. The major challenges in using ZnO for bio-sensing are attaining enhanced sensitivity and specificity. In this study, we explore the functionalization of zinc in ZnO through utilizing the thiol bond. The purpose of this study is to demonstrate that the ZnO based sensor is capable of achieving high specificity in presence of competitive surface binding through the thiol bond. The final goal is to design an ultra-specific biosensor to detect low occurring biomolecules. In this study, we have selected cortisol as a stress marker to demonstrate quantification and detection from synthetic sweat. In order to demonstrate ultra-specificity, we have used two competitive thiol based molecules binding to zinc, a linker Dithiobis succinimidyl propionate (DSP) and reducing agent of DSP, Dithiothreitol (DTT). Electrochemical impedance spectroscopy (EIS) is used to quantify the signal obtained through various ratiometric concentrations of DSP and DTT. To validate the EIS study results, inherent fluorescence studies are done by mapping changes in green emission spectrum of ZnO before and after linker functionalization. The optimal combination in terms of highest signal is identified to be of 25mM DTT and 50mM DSP. This is implemented in the experiments performed to calibrate the cortisol concentration in synthetic sweat. This study demonstrates the detection of cortisol antigen in synthetic sweat present within the physiological levels of 8 ng/mL to 140 ng/mL. Wangzhou, Andi verfasserin aut Selvam, Anjan Panneer verfasserin aut Muthukumar, Sriram verfasserin aut Prasad, Shalini verfasserin aut Enthalten in MRS online proceedings library Warrendale, Pa. : MRS, 1998 1720(2014), 1 vom: Dez., Seite 33-38 (DE-627)57782046X (DE-600)2451008-7 1946-4274 nnns volume:1720 year:2014 number:1 month:12 pages:33-38 https://dx.doi.org/10.1557/opl.2015.62 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_2005 AR 1720 2014 1 12 33-38 |
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10.1557/opl.2015.62 doi (DE-627)SPR042299217 (DE-599)SPRopl.2015.62-e (SPR)opl.2015.62-e DE-627 ger DE-627 rakwb eng 670 ASE Munje, Rujuta D. verfasserin aut Electrically Tunable Ultra-specific Zinc Oxide Biosensor 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Zinc oxide surface states can be utilized for ultra-specific detection of biomolecules. The major challenges in using ZnO for bio-sensing are attaining enhanced sensitivity and specificity. In this study, we explore the functionalization of zinc in ZnO through utilizing the thiol bond. The purpose of this study is to demonstrate that the ZnO based sensor is capable of achieving high specificity in presence of competitive surface binding through the thiol bond. The final goal is to design an ultra-specific biosensor to detect low occurring biomolecules. In this study, we have selected cortisol as a stress marker to demonstrate quantification and detection from synthetic sweat. In order to demonstrate ultra-specificity, we have used two competitive thiol based molecules binding to zinc, a linker Dithiobis succinimidyl propionate (DSP) and reducing agent of DSP, Dithiothreitol (DTT). Electrochemical impedance spectroscopy (EIS) is used to quantify the signal obtained through various ratiometric concentrations of DSP and DTT. To validate the EIS study results, inherent fluorescence studies are done by mapping changes in green emission spectrum of ZnO before and after linker functionalization. The optimal combination in terms of highest signal is identified to be of 25mM DTT and 50mM DSP. This is implemented in the experiments performed to calibrate the cortisol concentration in synthetic sweat. This study demonstrates the detection of cortisol antigen in synthetic sweat present within the physiological levels of 8 ng/mL to 140 ng/mL. Wangzhou, Andi verfasserin aut Selvam, Anjan Panneer verfasserin aut Muthukumar, Sriram verfasserin aut Prasad, Shalini verfasserin aut Enthalten in MRS online proceedings library Warrendale, Pa. : MRS, 1998 1720(2014), 1 vom: Dez., Seite 33-38 (DE-627)57782046X (DE-600)2451008-7 1946-4274 nnns volume:1720 year:2014 number:1 month:12 pages:33-38 https://dx.doi.org/10.1557/opl.2015.62 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_2005 AR 1720 2014 1 12 33-38 |
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10.1557/opl.2015.62 doi (DE-627)SPR042299217 (DE-599)SPRopl.2015.62-e (SPR)opl.2015.62-e DE-627 ger DE-627 rakwb eng 670 ASE Munje, Rujuta D. verfasserin aut Electrically Tunable Ultra-specific Zinc Oxide Biosensor 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Zinc oxide surface states can be utilized for ultra-specific detection of biomolecules. The major challenges in using ZnO for bio-sensing are attaining enhanced sensitivity and specificity. In this study, we explore the functionalization of zinc in ZnO through utilizing the thiol bond. The purpose of this study is to demonstrate that the ZnO based sensor is capable of achieving high specificity in presence of competitive surface binding through the thiol bond. The final goal is to design an ultra-specific biosensor to detect low occurring biomolecules. In this study, we have selected cortisol as a stress marker to demonstrate quantification and detection from synthetic sweat. In order to demonstrate ultra-specificity, we have used two competitive thiol based molecules binding to zinc, a linker Dithiobis succinimidyl propionate (DSP) and reducing agent of DSP, Dithiothreitol (DTT). Electrochemical impedance spectroscopy (EIS) is used to quantify the signal obtained through various ratiometric concentrations of DSP and DTT. To validate the EIS study results, inherent fluorescence studies are done by mapping changes in green emission spectrum of ZnO before and after linker functionalization. The optimal combination in terms of highest signal is identified to be of 25mM DTT and 50mM DSP. This is implemented in the experiments performed to calibrate the cortisol concentration in synthetic sweat. This study demonstrates the detection of cortisol antigen in synthetic sweat present within the physiological levels of 8 ng/mL to 140 ng/mL. Wangzhou, Andi verfasserin aut Selvam, Anjan Panneer verfasserin aut Muthukumar, Sriram verfasserin aut Prasad, Shalini verfasserin aut Enthalten in MRS online proceedings library Warrendale, Pa. : MRS, 1998 1720(2014), 1 vom: Dez., Seite 33-38 (DE-627)57782046X (DE-600)2451008-7 1946-4274 nnns volume:1720 year:2014 number:1 month:12 pages:33-38 https://dx.doi.org/10.1557/opl.2015.62 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_2005 AR 1720 2014 1 12 33-38 |
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Abstract Zinc oxide surface states can be utilized for ultra-specific detection of biomolecules. The major challenges in using ZnO for bio-sensing are attaining enhanced sensitivity and specificity. In this study, we explore the functionalization of zinc in ZnO through utilizing the thiol bond. The purpose of this study is to demonstrate that the ZnO based sensor is capable of achieving high specificity in presence of competitive surface binding through the thiol bond. The final goal is to design an ultra-specific biosensor to detect low occurring biomolecules. In this study, we have selected cortisol as a stress marker to demonstrate quantification and detection from synthetic sweat. In order to demonstrate ultra-specificity, we have used two competitive thiol based molecules binding to zinc, a linker Dithiobis succinimidyl propionate (DSP) and reducing agent of DSP, Dithiothreitol (DTT). Electrochemical impedance spectroscopy (EIS) is used to quantify the signal obtained through various ratiometric concentrations of DSP and DTT. To validate the EIS study results, inherent fluorescence studies are done by mapping changes in green emission spectrum of ZnO before and after linker functionalization. The optimal combination in terms of highest signal is identified to be of 25mM DTT and 50mM DSP. This is implemented in the experiments performed to calibrate the cortisol concentration in synthetic sweat. This study demonstrates the detection of cortisol antigen in synthetic sweat present within the physiological levels of 8 ng/mL to 140 ng/mL. |
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Abstract Zinc oxide surface states can be utilized for ultra-specific detection of biomolecules. The major challenges in using ZnO for bio-sensing are attaining enhanced sensitivity and specificity. In this study, we explore the functionalization of zinc in ZnO through utilizing the thiol bond. The purpose of this study is to demonstrate that the ZnO based sensor is capable of achieving high specificity in presence of competitive surface binding through the thiol bond. The final goal is to design an ultra-specific biosensor to detect low occurring biomolecules. In this study, we have selected cortisol as a stress marker to demonstrate quantification and detection from synthetic sweat. In order to demonstrate ultra-specificity, we have used two competitive thiol based molecules binding to zinc, a linker Dithiobis succinimidyl propionate (DSP) and reducing agent of DSP, Dithiothreitol (DTT). Electrochemical impedance spectroscopy (EIS) is used to quantify the signal obtained through various ratiometric concentrations of DSP and DTT. To validate the EIS study results, inherent fluorescence studies are done by mapping changes in green emission spectrum of ZnO before and after linker functionalization. The optimal combination in terms of highest signal is identified to be of 25mM DTT and 50mM DSP. This is implemented in the experiments performed to calibrate the cortisol concentration in synthetic sweat. This study demonstrates the detection of cortisol antigen in synthetic sweat present within the physiological levels of 8 ng/mL to 140 ng/mL. |
| abstract_unstemmed |
Abstract Zinc oxide surface states can be utilized for ultra-specific detection of biomolecules. The major challenges in using ZnO for bio-sensing are attaining enhanced sensitivity and specificity. In this study, we explore the functionalization of zinc in ZnO through utilizing the thiol bond. The purpose of this study is to demonstrate that the ZnO based sensor is capable of achieving high specificity in presence of competitive surface binding through the thiol bond. The final goal is to design an ultra-specific biosensor to detect low occurring biomolecules. In this study, we have selected cortisol as a stress marker to demonstrate quantification and detection from synthetic sweat. In order to demonstrate ultra-specificity, we have used two competitive thiol based molecules binding to zinc, a linker Dithiobis succinimidyl propionate (DSP) and reducing agent of DSP, Dithiothreitol (DTT). Electrochemical impedance spectroscopy (EIS) is used to quantify the signal obtained through various ratiometric concentrations of DSP and DTT. To validate the EIS study results, inherent fluorescence studies are done by mapping changes in green emission spectrum of ZnO before and after linker functionalization. The optimal combination in terms of highest signal is identified to be of 25mM DTT and 50mM DSP. This is implemented in the experiments performed to calibrate the cortisol concentration in synthetic sweat. This study demonstrates the detection of cortisol antigen in synthetic sweat present within the physiological levels of 8 ng/mL to 140 ng/mL. |
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Electrically Tunable Ultra-specific Zinc Oxide Biosensor |
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Wangzhou, Andi Selvam, Anjan Panneer Muthukumar, Sriram Prasad, Shalini |
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