In vitro micropropagation of Basella rubra L. through proliferation of axillary shoots
Abstract In vitro micropropagation protocol for Basella rubra regeneration was tried through proliferation of axillary shoots of the potted mature plant. The improved seed germination (70%) was recorded upon 2% urea treatment. The nodal shoot segments from matured potted plant were used to initiate...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Kumar, Sandopu Sravan [verfasserIn] Giridhar, Parvatam [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Plant cell, tissue and organ culture - Dordrecht [u.a.] : Springer Science + Business Media B.V, 1981, 144(2020), 2 vom: 30. Okt., Seite 477-483 |
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| Übergeordnetes Werk: |
volume:144 ; year:2020 ; number:2 ; day:30 ; month:10 ; pages:477-483 |
| Links: |
|---|
| DOI / URN: |
10.1007/s11240-020-01960-w |
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| Katalog-ID: |
SPR042963982 |
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| 100 | 1 | |a Kumar, Sandopu Sravan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a In vitro micropropagation of Basella rubra L. through proliferation of axillary shoots |
| 264 | 1 | |c 2020 | |
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| 520 | |a Abstract In vitro micropropagation protocol for Basella rubra regeneration was tried through proliferation of axillary shoots of the potted mature plant. The improved seed germination (70%) was recorded upon 2% urea treatment. The nodal shoot segments from matured potted plant were used to initiate the multiple shoot proliferation. The shoot segments exhibited 70% shoot initiation when cultured on Murashige and Skoog (MS) medium supplemented with Indole-3-acetic acid (IAA) + $ N_{6} $ – Benzylaminopurine (BAP) (0.25 + 2.0 mg/L) and BAP + Kinetin (Kin) (2.0 + 0.5 mg/L) respectively. Multiple shoots (5–6) were obtained on MS medium supplemented with BAP + Kin and IAA + BAP respectively. When compared with silver nitrate ($ AgNO_{3} $) (2–40 µM) and activated charcoal (AC) (0.1–1.0%), the MS medium devoid of any plant growth regulator showed good number of shoots (5.48 ± 2.42), elongation (15.64 ± 2.42 cm) and root length (14.52 ± 2.78 cm). Upon transferring of regenerated microshoots to MS medium, simultaneous elongation of shoots with more shoot number, shoot length and rooting was achieved during four subcultures that carried out at 6 weeks’ interval. The regenerated in vitro shoots showed 100% rooting in MS medium and also in MS medium supplemented with 0.1–1.0% AC. Hundred percent survival of micropropagated shoots well rooted was established successfully under greenhouse condition and the plants were subsequently acclimatized and transferred to the field conditions wherein 90% success rate was noted. | ||
| 520 | |a Key message We established a novel, and efficient protocol for in vitro B. rubra shoot cultures further multiplication in MS basal medium without any plant growth regulators with 100% elongation and rooting of microshoots that will overcome the use of synthetic hormones for improved micropropagation. | ||
| 650 | 4 | |a Micropropagation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Adventitious rooting |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Microshoots |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Germination |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Giridhar, Parvatam |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Plant cell, tissue and organ culture |d Dordrecht [u.a.] : Springer Science + Business Media B.V, 1981 |g 144(2020), 2 vom: 30. Okt., Seite 477-483 |w (DE-627)27093278X |w (DE-600)1478391-5 |x 1573-5044 |7 nnns |
| 773 | 1 | 8 | |g volume:144 |g year:2020 |g number:2 |g day:30 |g month:10 |g pages:477-483 |
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10.1007/s11240-020-01960-w doi (DE-627)SPR042963982 (DE-599)SPRs11240-020-01960-w-e (SPR)s11240-020-01960-w-e DE-627 ger DE-627 rakwb eng 630 640 570 540 ASE 42.03 bkl 42.40 bkl 48.03 bkl 48.56 bkl Kumar, Sandopu Sravan verfasserin aut In vitro micropropagation of Basella rubra L. through proliferation of axillary shoots 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In vitro micropropagation protocol for Basella rubra regeneration was tried through proliferation of axillary shoots of the potted mature plant. The improved seed germination (70%) was recorded upon 2% urea treatment. The nodal shoot segments from matured potted plant were used to initiate the multiple shoot proliferation. The shoot segments exhibited 70% shoot initiation when cultured on Murashige and Skoog (MS) medium supplemented with Indole-3-acetic acid (IAA) + $ N_{6} $ – Benzylaminopurine (BAP) (0.25 + 2.0 mg/L) and BAP + Kinetin (Kin) (2.0 + 0.5 mg/L) respectively. Multiple shoots (5–6) were obtained on MS medium supplemented with BAP + Kin and IAA + BAP respectively. When compared with silver nitrate ($ AgNO_{3} $) (2–40 µM) and activated charcoal (AC) (0.1–1.0%), the MS medium devoid of any plant growth regulator showed good number of shoots (5.48 ± 2.42), elongation (15.64 ± 2.42 cm) and root length (14.52 ± 2.78 cm). Upon transferring of regenerated microshoots to MS medium, simultaneous elongation of shoots with more shoot number, shoot length and rooting was achieved during four subcultures that carried out at 6 weeks’ interval. The regenerated in vitro shoots showed 100% rooting in MS medium and also in MS medium supplemented with 0.1–1.0% AC. Hundred percent survival of micropropagated shoots well rooted was established successfully under greenhouse condition and the plants were subsequently acclimatized and transferred to the field conditions wherein 90% success rate was noted. Key message We established a novel, and efficient protocol for in vitro B. rubra shoot cultures further multiplication in MS basal medium without any plant growth regulators with 100% elongation and rooting of microshoots that will overcome the use of synthetic hormones for improved micropropagation. Micropropagation (dpeaa)DE-He213 Adventitious rooting (dpeaa)DE-He213 Microshoots (dpeaa)DE-He213 Germination (dpeaa)DE-He213 Giridhar, Parvatam verfasserin aut Enthalten in Plant cell, tissue and organ culture Dordrecht [u.a.] : Springer Science + Business Media B.V, 1981 144(2020), 2 vom: 30. Okt., Seite 477-483 (DE-627)27093278X (DE-600)1478391-5 1573-5044 nnns volume:144 year:2020 number:2 day:30 month:10 pages:477-483 https://dx.doi.org/10.1007/s11240-020-01960-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-FOR SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.03 ASE 42.40 ASE 48.03 ASE 48.56 ASE AR 144 2020 2 30 10 477-483 |
| spelling |
10.1007/s11240-020-01960-w doi (DE-627)SPR042963982 (DE-599)SPRs11240-020-01960-w-e (SPR)s11240-020-01960-w-e DE-627 ger DE-627 rakwb eng 630 640 570 540 ASE 42.03 bkl 42.40 bkl 48.03 bkl 48.56 bkl Kumar, Sandopu Sravan verfasserin aut In vitro micropropagation of Basella rubra L. through proliferation of axillary shoots 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In vitro micropropagation protocol for Basella rubra regeneration was tried through proliferation of axillary shoots of the potted mature plant. The improved seed germination (70%) was recorded upon 2% urea treatment. The nodal shoot segments from matured potted plant were used to initiate the multiple shoot proliferation. The shoot segments exhibited 70% shoot initiation when cultured on Murashige and Skoog (MS) medium supplemented with Indole-3-acetic acid (IAA) + $ N_{6} $ – Benzylaminopurine (BAP) (0.25 + 2.0 mg/L) and BAP + Kinetin (Kin) (2.0 + 0.5 mg/L) respectively. Multiple shoots (5–6) were obtained on MS medium supplemented with BAP + Kin and IAA + BAP respectively. When compared with silver nitrate ($ AgNO_{3} $) (2–40 µM) and activated charcoal (AC) (0.1–1.0%), the MS medium devoid of any plant growth regulator showed good number of shoots (5.48 ± 2.42), elongation (15.64 ± 2.42 cm) and root length (14.52 ± 2.78 cm). Upon transferring of regenerated microshoots to MS medium, simultaneous elongation of shoots with more shoot number, shoot length and rooting was achieved during four subcultures that carried out at 6 weeks’ interval. The regenerated in vitro shoots showed 100% rooting in MS medium and also in MS medium supplemented with 0.1–1.0% AC. Hundred percent survival of micropropagated shoots well rooted was established successfully under greenhouse condition and the plants were subsequently acclimatized and transferred to the field conditions wherein 90% success rate was noted. Key message We established a novel, and efficient protocol for in vitro B. rubra shoot cultures further multiplication in MS basal medium without any plant growth regulators with 100% elongation and rooting of microshoots that will overcome the use of synthetic hormones for improved micropropagation. Micropropagation (dpeaa)DE-He213 Adventitious rooting (dpeaa)DE-He213 Microshoots (dpeaa)DE-He213 Germination (dpeaa)DE-He213 Giridhar, Parvatam verfasserin aut Enthalten in Plant cell, tissue and organ culture Dordrecht [u.a.] : Springer Science + Business Media B.V, 1981 144(2020), 2 vom: 30. Okt., Seite 477-483 (DE-627)27093278X (DE-600)1478391-5 1573-5044 nnns volume:144 year:2020 number:2 day:30 month:10 pages:477-483 https://dx.doi.org/10.1007/s11240-020-01960-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-FOR SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.03 ASE 42.40 ASE 48.03 ASE 48.56 ASE AR 144 2020 2 30 10 477-483 |
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10.1007/s11240-020-01960-w doi (DE-627)SPR042963982 (DE-599)SPRs11240-020-01960-w-e (SPR)s11240-020-01960-w-e DE-627 ger DE-627 rakwb eng 630 640 570 540 ASE 42.03 bkl 42.40 bkl 48.03 bkl 48.56 bkl Kumar, Sandopu Sravan verfasserin aut In vitro micropropagation of Basella rubra L. through proliferation of axillary shoots 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In vitro micropropagation protocol for Basella rubra regeneration was tried through proliferation of axillary shoots of the potted mature plant. The improved seed germination (70%) was recorded upon 2% urea treatment. The nodal shoot segments from matured potted plant were used to initiate the multiple shoot proliferation. The shoot segments exhibited 70% shoot initiation when cultured on Murashige and Skoog (MS) medium supplemented with Indole-3-acetic acid (IAA) + $ N_{6} $ – Benzylaminopurine (BAP) (0.25 + 2.0 mg/L) and BAP + Kinetin (Kin) (2.0 + 0.5 mg/L) respectively. Multiple shoots (5–6) were obtained on MS medium supplemented with BAP + Kin and IAA + BAP respectively. When compared with silver nitrate ($ AgNO_{3} $) (2–40 µM) and activated charcoal (AC) (0.1–1.0%), the MS medium devoid of any plant growth regulator showed good number of shoots (5.48 ± 2.42), elongation (15.64 ± 2.42 cm) and root length (14.52 ± 2.78 cm). Upon transferring of regenerated microshoots to MS medium, simultaneous elongation of shoots with more shoot number, shoot length and rooting was achieved during four subcultures that carried out at 6 weeks’ interval. The regenerated in vitro shoots showed 100% rooting in MS medium and also in MS medium supplemented with 0.1–1.0% AC. Hundred percent survival of micropropagated shoots well rooted was established successfully under greenhouse condition and the plants were subsequently acclimatized and transferred to the field conditions wherein 90% success rate was noted. Key message We established a novel, and efficient protocol for in vitro B. rubra shoot cultures further multiplication in MS basal medium without any plant growth regulators with 100% elongation and rooting of microshoots that will overcome the use of synthetic hormones for improved micropropagation. Micropropagation (dpeaa)DE-He213 Adventitious rooting (dpeaa)DE-He213 Microshoots (dpeaa)DE-He213 Germination (dpeaa)DE-He213 Giridhar, Parvatam verfasserin aut Enthalten in Plant cell, tissue and organ culture Dordrecht [u.a.] : Springer Science + Business Media B.V, 1981 144(2020), 2 vom: 30. Okt., Seite 477-483 (DE-627)27093278X (DE-600)1478391-5 1573-5044 nnns volume:144 year:2020 number:2 day:30 month:10 pages:477-483 https://dx.doi.org/10.1007/s11240-020-01960-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-FOR SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.03 ASE 42.40 ASE 48.03 ASE 48.56 ASE AR 144 2020 2 30 10 477-483 |
| allfieldsGer |
10.1007/s11240-020-01960-w doi (DE-627)SPR042963982 (DE-599)SPRs11240-020-01960-w-e (SPR)s11240-020-01960-w-e DE-627 ger DE-627 rakwb eng 630 640 570 540 ASE 42.03 bkl 42.40 bkl 48.03 bkl 48.56 bkl Kumar, Sandopu Sravan verfasserin aut In vitro micropropagation of Basella rubra L. through proliferation of axillary shoots 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In vitro micropropagation protocol for Basella rubra regeneration was tried through proliferation of axillary shoots of the potted mature plant. The improved seed germination (70%) was recorded upon 2% urea treatment. The nodal shoot segments from matured potted plant were used to initiate the multiple shoot proliferation. The shoot segments exhibited 70% shoot initiation when cultured on Murashige and Skoog (MS) medium supplemented with Indole-3-acetic acid (IAA) + $ N_{6} $ – Benzylaminopurine (BAP) (0.25 + 2.0 mg/L) and BAP + Kinetin (Kin) (2.0 + 0.5 mg/L) respectively. Multiple shoots (5–6) were obtained on MS medium supplemented with BAP + Kin and IAA + BAP respectively. When compared with silver nitrate ($ AgNO_{3} $) (2–40 µM) and activated charcoal (AC) (0.1–1.0%), the MS medium devoid of any plant growth regulator showed good number of shoots (5.48 ± 2.42), elongation (15.64 ± 2.42 cm) and root length (14.52 ± 2.78 cm). Upon transferring of regenerated microshoots to MS medium, simultaneous elongation of shoots with more shoot number, shoot length and rooting was achieved during four subcultures that carried out at 6 weeks’ interval. The regenerated in vitro shoots showed 100% rooting in MS medium and also in MS medium supplemented with 0.1–1.0% AC. Hundred percent survival of micropropagated shoots well rooted was established successfully under greenhouse condition and the plants were subsequently acclimatized and transferred to the field conditions wherein 90% success rate was noted. Key message We established a novel, and efficient protocol for in vitro B. rubra shoot cultures further multiplication in MS basal medium without any plant growth regulators with 100% elongation and rooting of microshoots that will overcome the use of synthetic hormones for improved micropropagation. Micropropagation (dpeaa)DE-He213 Adventitious rooting (dpeaa)DE-He213 Microshoots (dpeaa)DE-He213 Germination (dpeaa)DE-He213 Giridhar, Parvatam verfasserin aut Enthalten in Plant cell, tissue and organ culture Dordrecht [u.a.] : Springer Science + Business Media B.V, 1981 144(2020), 2 vom: 30. Okt., Seite 477-483 (DE-627)27093278X (DE-600)1478391-5 1573-5044 nnns volume:144 year:2020 number:2 day:30 month:10 pages:477-483 https://dx.doi.org/10.1007/s11240-020-01960-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-FOR SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.03 ASE 42.40 ASE 48.03 ASE 48.56 ASE AR 144 2020 2 30 10 477-483 |
| allfieldsSound |
10.1007/s11240-020-01960-w doi (DE-627)SPR042963982 (DE-599)SPRs11240-020-01960-w-e (SPR)s11240-020-01960-w-e DE-627 ger DE-627 rakwb eng 630 640 570 540 ASE 42.03 bkl 42.40 bkl 48.03 bkl 48.56 bkl Kumar, Sandopu Sravan verfasserin aut In vitro micropropagation of Basella rubra L. through proliferation of axillary shoots 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In vitro micropropagation protocol for Basella rubra regeneration was tried through proliferation of axillary shoots of the potted mature plant. The improved seed germination (70%) was recorded upon 2% urea treatment. The nodal shoot segments from matured potted plant were used to initiate the multiple shoot proliferation. The shoot segments exhibited 70% shoot initiation when cultured on Murashige and Skoog (MS) medium supplemented with Indole-3-acetic acid (IAA) + $ N_{6} $ – Benzylaminopurine (BAP) (0.25 + 2.0 mg/L) and BAP + Kinetin (Kin) (2.0 + 0.5 mg/L) respectively. Multiple shoots (5–6) were obtained on MS medium supplemented with BAP + Kin and IAA + BAP respectively. When compared with silver nitrate ($ AgNO_{3} $) (2–40 µM) and activated charcoal (AC) (0.1–1.0%), the MS medium devoid of any plant growth regulator showed good number of shoots (5.48 ± 2.42), elongation (15.64 ± 2.42 cm) and root length (14.52 ± 2.78 cm). Upon transferring of regenerated microshoots to MS medium, simultaneous elongation of shoots with more shoot number, shoot length and rooting was achieved during four subcultures that carried out at 6 weeks’ interval. The regenerated in vitro shoots showed 100% rooting in MS medium and also in MS medium supplemented with 0.1–1.0% AC. Hundred percent survival of micropropagated shoots well rooted was established successfully under greenhouse condition and the plants were subsequently acclimatized and transferred to the field conditions wherein 90% success rate was noted. Key message We established a novel, and efficient protocol for in vitro B. rubra shoot cultures further multiplication in MS basal medium without any plant growth regulators with 100% elongation and rooting of microshoots that will overcome the use of synthetic hormones for improved micropropagation. Micropropagation (dpeaa)DE-He213 Adventitious rooting (dpeaa)DE-He213 Microshoots (dpeaa)DE-He213 Germination (dpeaa)DE-He213 Giridhar, Parvatam verfasserin aut Enthalten in Plant cell, tissue and organ culture Dordrecht [u.a.] : Springer Science + Business Media B.V, 1981 144(2020), 2 vom: 30. Okt., Seite 477-483 (DE-627)27093278X (DE-600)1478391-5 1573-5044 nnns volume:144 year:2020 number:2 day:30 month:10 pages:477-483 https://dx.doi.org/10.1007/s11240-020-01960-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-FOR SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.03 ASE 42.40 ASE 48.03 ASE 48.56 ASE AR 144 2020 2 30 10 477-483 |
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English |
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Enthalten in Plant cell, tissue and organ culture 144(2020), 2 vom: 30. Okt., Seite 477-483 volume:144 year:2020 number:2 day:30 month:10 pages:477-483 |
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Enthalten in Plant cell, tissue and organ culture 144(2020), 2 vom: 30. Okt., Seite 477-483 volume:144 year:2020 number:2 day:30 month:10 pages:477-483 |
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Micropropagation Adventitious rooting Microshoots Germination |
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Plant cell, tissue and organ culture |
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Kumar, Sandopu Sravan @@aut@@ Giridhar, Parvatam @@aut@@ |
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2020-10-30T00:00:00Z |
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The improved seed germination (70%) was recorded upon 2% urea treatment. The nodal shoot segments from matured potted plant were used to initiate the multiple shoot proliferation. The shoot segments exhibited 70% shoot initiation when cultured on Murashige and Skoog (MS) medium supplemented with Indole-3-acetic acid (IAA) + $ N_{6} $ – Benzylaminopurine (BAP) (0.25 + 2.0 mg/L) and BAP + Kinetin (Kin) (2.0 + 0.5 mg/L) respectively. Multiple shoots (5–6) were obtained on MS medium supplemented with BAP + Kin and IAA + BAP respectively. When compared with silver nitrate ($ AgNO_{3} $) (2–40 µM) and activated charcoal (AC) (0.1–1.0%), the MS medium devoid of any plant growth regulator showed good number of shoots (5.48 ± 2.42), elongation (15.64 ± 2.42 cm) and root length (14.52 ± 2.78 cm). Upon transferring of regenerated microshoots to MS medium, simultaneous elongation of shoots with more shoot number, shoot length and rooting was achieved during four subcultures that carried out at 6 weeks’ interval. The regenerated in vitro shoots showed 100% rooting in MS medium and also in MS medium supplemented with 0.1–1.0% AC. Hundred percent survival of micropropagated shoots well rooted was established successfully under greenhouse condition and the plants were subsequently acclimatized and transferred to the field conditions wherein 90% success rate was noted.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Key message We established a novel, and efficient protocol for in vitro B. rubra shoot cultures further multiplication in MS basal medium without any plant growth regulators with 100% elongation and rooting of microshoots that will overcome the use of synthetic hormones for improved micropropagation.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Micropropagation</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Adventitious rooting</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Microshoots</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Germination</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Giridhar, Parvatam</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Plant cell, tissue and organ culture</subfield><subfield code="d">Dordrecht [u.a.] : Springer Science + Business Media B.V, 1981</subfield><subfield code="g">144(2020), 2 vom: 30. 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Kumar, Sandopu Sravan |
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Kumar, Sandopu Sravan ddc 630 bkl 42.03 bkl 42.40 bkl 48.03 bkl 48.56 misc Micropropagation misc Adventitious rooting misc Microshoots misc Germination In vitro micropropagation of Basella rubra L. through proliferation of axillary shoots |
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630 640 570 540 ASE 42.03 bkl 42.40 bkl 48.03 bkl 48.56 bkl In vitro micropropagation of Basella rubra L. through proliferation of axillary shoots Micropropagation (dpeaa)DE-He213 Adventitious rooting (dpeaa)DE-He213 Microshoots (dpeaa)DE-He213 Germination (dpeaa)DE-He213 |
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ddc 630 bkl 42.03 bkl 42.40 bkl 48.03 bkl 48.56 misc Micropropagation misc Adventitious rooting misc Microshoots misc Germination |
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ddc 630 bkl 42.03 bkl 42.40 bkl 48.03 bkl 48.56 misc Micropropagation misc Adventitious rooting misc Microshoots misc Germination |
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ddc 630 bkl 42.03 bkl 42.40 bkl 48.03 bkl 48.56 misc Micropropagation misc Adventitious rooting misc Microshoots misc Germination |
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In vitro micropropagation of Basella rubra L. through proliferation of axillary shoots |
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In vitro micropropagation of Basella rubra L. through proliferation of axillary shoots |
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Kumar, Sandopu Sravan |
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Plant cell, tissue and organ culture |
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Plant cell, tissue and organ culture |
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Kumar, Sandopu Sravan Giridhar, Parvatam |
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Elektronische Aufsätze |
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Kumar, Sandopu Sravan |
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in vitro micropropagation of basella rubra l. through proliferation of axillary shoots |
| title_auth |
In vitro micropropagation of Basella rubra L. through proliferation of axillary shoots |
| abstract |
Abstract In vitro micropropagation protocol for Basella rubra regeneration was tried through proliferation of axillary shoots of the potted mature plant. The improved seed germination (70%) was recorded upon 2% urea treatment. The nodal shoot segments from matured potted plant were used to initiate the multiple shoot proliferation. The shoot segments exhibited 70% shoot initiation when cultured on Murashige and Skoog (MS) medium supplemented with Indole-3-acetic acid (IAA) + $ N_{6} $ – Benzylaminopurine (BAP) (0.25 + 2.0 mg/L) and BAP + Kinetin (Kin) (2.0 + 0.5 mg/L) respectively. Multiple shoots (5–6) were obtained on MS medium supplemented with BAP + Kin and IAA + BAP respectively. When compared with silver nitrate ($ AgNO_{3} $) (2–40 µM) and activated charcoal (AC) (0.1–1.0%), the MS medium devoid of any plant growth regulator showed good number of shoots (5.48 ± 2.42), elongation (15.64 ± 2.42 cm) and root length (14.52 ± 2.78 cm). Upon transferring of regenerated microshoots to MS medium, simultaneous elongation of shoots with more shoot number, shoot length and rooting was achieved during four subcultures that carried out at 6 weeks’ interval. The regenerated in vitro shoots showed 100% rooting in MS medium and also in MS medium supplemented with 0.1–1.0% AC. Hundred percent survival of micropropagated shoots well rooted was established successfully under greenhouse condition and the plants were subsequently acclimatized and transferred to the field conditions wherein 90% success rate was noted. Key message We established a novel, and efficient protocol for in vitro B. rubra shoot cultures further multiplication in MS basal medium without any plant growth regulators with 100% elongation and rooting of microshoots that will overcome the use of synthetic hormones for improved micropropagation. |
| abstractGer |
Abstract In vitro micropropagation protocol for Basella rubra regeneration was tried through proliferation of axillary shoots of the potted mature plant. The improved seed germination (70%) was recorded upon 2% urea treatment. The nodal shoot segments from matured potted plant were used to initiate the multiple shoot proliferation. The shoot segments exhibited 70% shoot initiation when cultured on Murashige and Skoog (MS) medium supplemented with Indole-3-acetic acid (IAA) + $ N_{6} $ – Benzylaminopurine (BAP) (0.25 + 2.0 mg/L) and BAP + Kinetin (Kin) (2.0 + 0.5 mg/L) respectively. Multiple shoots (5–6) were obtained on MS medium supplemented with BAP + Kin and IAA + BAP respectively. When compared with silver nitrate ($ AgNO_{3} $) (2–40 µM) and activated charcoal (AC) (0.1–1.0%), the MS medium devoid of any plant growth regulator showed good number of shoots (5.48 ± 2.42), elongation (15.64 ± 2.42 cm) and root length (14.52 ± 2.78 cm). Upon transferring of regenerated microshoots to MS medium, simultaneous elongation of shoots with more shoot number, shoot length and rooting was achieved during four subcultures that carried out at 6 weeks’ interval. The regenerated in vitro shoots showed 100% rooting in MS medium and also in MS medium supplemented with 0.1–1.0% AC. Hundred percent survival of micropropagated shoots well rooted was established successfully under greenhouse condition and the plants were subsequently acclimatized and transferred to the field conditions wherein 90% success rate was noted. Key message We established a novel, and efficient protocol for in vitro B. rubra shoot cultures further multiplication in MS basal medium without any plant growth regulators with 100% elongation and rooting of microshoots that will overcome the use of synthetic hormones for improved micropropagation. |
| abstract_unstemmed |
Abstract In vitro micropropagation protocol for Basella rubra regeneration was tried through proliferation of axillary shoots of the potted mature plant. The improved seed germination (70%) was recorded upon 2% urea treatment. The nodal shoot segments from matured potted plant were used to initiate the multiple shoot proliferation. The shoot segments exhibited 70% shoot initiation when cultured on Murashige and Skoog (MS) medium supplemented with Indole-3-acetic acid (IAA) + $ N_{6} $ – Benzylaminopurine (BAP) (0.25 + 2.0 mg/L) and BAP + Kinetin (Kin) (2.0 + 0.5 mg/L) respectively. Multiple shoots (5–6) were obtained on MS medium supplemented with BAP + Kin and IAA + BAP respectively. When compared with silver nitrate ($ AgNO_{3} $) (2–40 µM) and activated charcoal (AC) (0.1–1.0%), the MS medium devoid of any plant growth regulator showed good number of shoots (5.48 ± 2.42), elongation (15.64 ± 2.42 cm) and root length (14.52 ± 2.78 cm). Upon transferring of regenerated microshoots to MS medium, simultaneous elongation of shoots with more shoot number, shoot length and rooting was achieved during four subcultures that carried out at 6 weeks’ interval. The regenerated in vitro shoots showed 100% rooting in MS medium and also in MS medium supplemented with 0.1–1.0% AC. Hundred percent survival of micropropagated shoots well rooted was established successfully under greenhouse condition and the plants were subsequently acclimatized and transferred to the field conditions wherein 90% success rate was noted. Key message We established a novel, and efficient protocol for in vitro B. rubra shoot cultures further multiplication in MS basal medium without any plant growth regulators with 100% elongation and rooting of microshoots that will overcome the use of synthetic hormones for improved micropropagation. |
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In vitro micropropagation of Basella rubra L. through proliferation of axillary shoots |
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| score |
7.3998976 |