Pediatric low-grade gliomas: molecular characterization of patient-derived cellular models
Purpose Pediatric low-grade gliomas (pLGGs), the most frequent pediatric brain tumors, include different entities harboring distinct histological and molecular features. A major limitation in the development of treatments for these tumors is the absence of reliable in vitro models that would allow a...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Chiacchiarini, Martina [verfasserIn] Besharat, Zein Mersini [verfasserIn] Carai, Andrea [verfasserIn] Miele, Evelina [verfasserIn] Del Baldo, Giada [verfasserIn] Mastronuzzi, Angela [verfasserIn] Catanzaro, Giuseppina [verfasserIn] Ferretti, Elisabetta [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Child's nervous system - Berlin : Springer, 1985, 37(2020), 3 vom: 11. März, Seite 771-778 |
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| Übergeordnetes Werk: |
volume:37 ; year:2020 ; number:3 ; day:11 ; month:03 ; pages:771-778 |
| Links: |
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| DOI / URN: |
10.1007/s00381-020-04559-w |
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| Katalog-ID: |
SPR043108539 |
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| 520 | |a Purpose Pediatric low-grade gliomas (pLGGs), the most frequent pediatric brain tumors, include different entities harboring distinct histological and molecular features. A major limitation in the development of treatments for these tumors is the absence of reliable in vitro models that would allow a better understanding of the molecular mechanisms that support their growth. Surgical excision is the primary treatment method and the extent of resection represents one of the strongest prognostic factors. pLGGs that cannot be completely resected are prone to recur and associated with relapses and extensive morbidities, thus remaining a major clinical challenge. Methods We established a protocol to successfully derive primary patient-derived pLGG cells and to fully characterize them from a molecular point of view. Results Primary patients-derived pLGG cells were extensively analyzed in order to confirm their reliability as cellular models. Specifically, we evaluated the growth rate, senescence, and molecular features, such as BRAF mutational status, methylation, and protein expression profile. Conclusion This study extensively describes pLGG primary cellular models in terms of isolation, culture method, and molecular characterization that can be used to investigate pLGG biology. | ||
| 650 | 4 | |a Patients derived primary cellular models |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Pediatric low-grade gliomas |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Molecular characterization |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Methylation |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Besharat, Zein Mersini |e verfasserin |4 aut | |
| 700 | 1 | |a Carai, Andrea |e verfasserin |4 aut | |
| 700 | 1 | |a Miele, Evelina |e verfasserin |4 aut | |
| 700 | 1 | |a Del Baldo, Giada |e verfasserin |4 aut | |
| 700 | 1 | |a Mastronuzzi, Angela |e verfasserin |4 aut | |
| 700 | 1 | |a Catanzaro, Giuseppina |e verfasserin |4 aut | |
| 700 | 1 | |a Ferretti, Elisabetta |e verfasserin |4 aut | |
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10.1007/s00381-020-04559-w doi (DE-627)SPR043108539 (DE-599)SPRs00381-020-04559-w-e (SPR)s00381-020-04559-w-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl 44.67 bkl Chiacchiarini, Martina verfasserin aut Pediatric low-grade gliomas: molecular characterization of patient-derived cellular models 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purpose Pediatric low-grade gliomas (pLGGs), the most frequent pediatric brain tumors, include different entities harboring distinct histological and molecular features. A major limitation in the development of treatments for these tumors is the absence of reliable in vitro models that would allow a better understanding of the molecular mechanisms that support their growth. Surgical excision is the primary treatment method and the extent of resection represents one of the strongest prognostic factors. pLGGs that cannot be completely resected are prone to recur and associated with relapses and extensive morbidities, thus remaining a major clinical challenge. Methods We established a protocol to successfully derive primary patient-derived pLGG cells and to fully characterize them from a molecular point of view. Results Primary patients-derived pLGG cells were extensively analyzed in order to confirm their reliability as cellular models. Specifically, we evaluated the growth rate, senescence, and molecular features, such as BRAF mutational status, methylation, and protein expression profile. Conclusion This study extensively describes pLGG primary cellular models in terms of isolation, culture method, and molecular characterization that can be used to investigate pLGG biology. Patients derived primary cellular models (dpeaa)DE-He213 Pediatric low-grade gliomas (dpeaa)DE-He213 Molecular characterization (dpeaa)DE-He213 Methylation (dpeaa)DE-He213 Besharat, Zein Mersini verfasserin aut Carai, Andrea verfasserin aut Miele, Evelina verfasserin aut Del Baldo, Giada verfasserin aut Mastronuzzi, Angela verfasserin aut Catanzaro, Giuseppina verfasserin aut Ferretti, Elisabetta verfasserin aut Enthalten in Child's nervous system Berlin : Springer, 1985 37(2020), 3 vom: 11. März, Seite 771-778 (DE-627)254639054 (DE-600)1463024-2 1433-0350 nnns volume:37 year:2020 number:3 day:11 month:03 pages:771-778 https://dx.doi.org/10.1007/s00381-020-04559-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE 44.67 ASE AR 37 2020 3 11 03 771-778 |
| spelling |
10.1007/s00381-020-04559-w doi (DE-627)SPR043108539 (DE-599)SPRs00381-020-04559-w-e (SPR)s00381-020-04559-w-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl 44.67 bkl Chiacchiarini, Martina verfasserin aut Pediatric low-grade gliomas: molecular characterization of patient-derived cellular models 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purpose Pediatric low-grade gliomas (pLGGs), the most frequent pediatric brain tumors, include different entities harboring distinct histological and molecular features. A major limitation in the development of treatments for these tumors is the absence of reliable in vitro models that would allow a better understanding of the molecular mechanisms that support their growth. Surgical excision is the primary treatment method and the extent of resection represents one of the strongest prognostic factors. pLGGs that cannot be completely resected are prone to recur and associated with relapses and extensive morbidities, thus remaining a major clinical challenge. Methods We established a protocol to successfully derive primary patient-derived pLGG cells and to fully characterize them from a molecular point of view. Results Primary patients-derived pLGG cells were extensively analyzed in order to confirm their reliability as cellular models. Specifically, we evaluated the growth rate, senescence, and molecular features, such as BRAF mutational status, methylation, and protein expression profile. Conclusion This study extensively describes pLGG primary cellular models in terms of isolation, culture method, and molecular characterization that can be used to investigate pLGG biology. Patients derived primary cellular models (dpeaa)DE-He213 Pediatric low-grade gliomas (dpeaa)DE-He213 Molecular characterization (dpeaa)DE-He213 Methylation (dpeaa)DE-He213 Besharat, Zein Mersini verfasserin aut Carai, Andrea verfasserin aut Miele, Evelina verfasserin aut Del Baldo, Giada verfasserin aut Mastronuzzi, Angela verfasserin aut Catanzaro, Giuseppina verfasserin aut Ferretti, Elisabetta verfasserin aut Enthalten in Child's nervous system Berlin : Springer, 1985 37(2020), 3 vom: 11. März, Seite 771-778 (DE-627)254639054 (DE-600)1463024-2 1433-0350 nnns volume:37 year:2020 number:3 day:11 month:03 pages:771-778 https://dx.doi.org/10.1007/s00381-020-04559-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE 44.67 ASE AR 37 2020 3 11 03 771-778 |
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10.1007/s00381-020-04559-w doi (DE-627)SPR043108539 (DE-599)SPRs00381-020-04559-w-e (SPR)s00381-020-04559-w-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl 44.67 bkl Chiacchiarini, Martina verfasserin aut Pediatric low-grade gliomas: molecular characterization of patient-derived cellular models 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purpose Pediatric low-grade gliomas (pLGGs), the most frequent pediatric brain tumors, include different entities harboring distinct histological and molecular features. A major limitation in the development of treatments for these tumors is the absence of reliable in vitro models that would allow a better understanding of the molecular mechanisms that support their growth. Surgical excision is the primary treatment method and the extent of resection represents one of the strongest prognostic factors. pLGGs that cannot be completely resected are prone to recur and associated with relapses and extensive morbidities, thus remaining a major clinical challenge. Methods We established a protocol to successfully derive primary patient-derived pLGG cells and to fully characterize them from a molecular point of view. Results Primary patients-derived pLGG cells were extensively analyzed in order to confirm their reliability as cellular models. Specifically, we evaluated the growth rate, senescence, and molecular features, such as BRAF mutational status, methylation, and protein expression profile. Conclusion This study extensively describes pLGG primary cellular models in terms of isolation, culture method, and molecular characterization that can be used to investigate pLGG biology. Patients derived primary cellular models (dpeaa)DE-He213 Pediatric low-grade gliomas (dpeaa)DE-He213 Molecular characterization (dpeaa)DE-He213 Methylation (dpeaa)DE-He213 Besharat, Zein Mersini verfasserin aut Carai, Andrea verfasserin aut Miele, Evelina verfasserin aut Del Baldo, Giada verfasserin aut Mastronuzzi, Angela verfasserin aut Catanzaro, Giuseppina verfasserin aut Ferretti, Elisabetta verfasserin aut Enthalten in Child's nervous system Berlin : Springer, 1985 37(2020), 3 vom: 11. März, Seite 771-778 (DE-627)254639054 (DE-600)1463024-2 1433-0350 nnns volume:37 year:2020 number:3 day:11 month:03 pages:771-778 https://dx.doi.org/10.1007/s00381-020-04559-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE 44.67 ASE AR 37 2020 3 11 03 771-778 |
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10.1007/s00381-020-04559-w doi (DE-627)SPR043108539 (DE-599)SPRs00381-020-04559-w-e (SPR)s00381-020-04559-w-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl 44.67 bkl Chiacchiarini, Martina verfasserin aut Pediatric low-grade gliomas: molecular characterization of patient-derived cellular models 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purpose Pediatric low-grade gliomas (pLGGs), the most frequent pediatric brain tumors, include different entities harboring distinct histological and molecular features. A major limitation in the development of treatments for these tumors is the absence of reliable in vitro models that would allow a better understanding of the molecular mechanisms that support their growth. Surgical excision is the primary treatment method and the extent of resection represents one of the strongest prognostic factors. pLGGs that cannot be completely resected are prone to recur and associated with relapses and extensive morbidities, thus remaining a major clinical challenge. Methods We established a protocol to successfully derive primary patient-derived pLGG cells and to fully characterize them from a molecular point of view. Results Primary patients-derived pLGG cells were extensively analyzed in order to confirm their reliability as cellular models. Specifically, we evaluated the growth rate, senescence, and molecular features, such as BRAF mutational status, methylation, and protein expression profile. Conclusion This study extensively describes pLGG primary cellular models in terms of isolation, culture method, and molecular characterization that can be used to investigate pLGG biology. Patients derived primary cellular models (dpeaa)DE-He213 Pediatric low-grade gliomas (dpeaa)DE-He213 Molecular characterization (dpeaa)DE-He213 Methylation (dpeaa)DE-He213 Besharat, Zein Mersini verfasserin aut Carai, Andrea verfasserin aut Miele, Evelina verfasserin aut Del Baldo, Giada verfasserin aut Mastronuzzi, Angela verfasserin aut Catanzaro, Giuseppina verfasserin aut Ferretti, Elisabetta verfasserin aut Enthalten in Child's nervous system Berlin : Springer, 1985 37(2020), 3 vom: 11. März, Seite 771-778 (DE-627)254639054 (DE-600)1463024-2 1433-0350 nnns volume:37 year:2020 number:3 day:11 month:03 pages:771-778 https://dx.doi.org/10.1007/s00381-020-04559-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE 44.67 ASE AR 37 2020 3 11 03 771-778 |
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10.1007/s00381-020-04559-w doi (DE-627)SPR043108539 (DE-599)SPRs00381-020-04559-w-e (SPR)s00381-020-04559-w-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl 44.67 bkl Chiacchiarini, Martina verfasserin aut Pediatric low-grade gliomas: molecular characterization of patient-derived cellular models 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purpose Pediatric low-grade gliomas (pLGGs), the most frequent pediatric brain tumors, include different entities harboring distinct histological and molecular features. A major limitation in the development of treatments for these tumors is the absence of reliable in vitro models that would allow a better understanding of the molecular mechanisms that support their growth. Surgical excision is the primary treatment method and the extent of resection represents one of the strongest prognostic factors. pLGGs that cannot be completely resected are prone to recur and associated with relapses and extensive morbidities, thus remaining a major clinical challenge. Methods We established a protocol to successfully derive primary patient-derived pLGG cells and to fully characterize them from a molecular point of view. Results Primary patients-derived pLGG cells were extensively analyzed in order to confirm their reliability as cellular models. Specifically, we evaluated the growth rate, senescence, and molecular features, such as BRAF mutational status, methylation, and protein expression profile. Conclusion This study extensively describes pLGG primary cellular models in terms of isolation, culture method, and molecular characterization that can be used to investigate pLGG biology. Patients derived primary cellular models (dpeaa)DE-He213 Pediatric low-grade gliomas (dpeaa)DE-He213 Molecular characterization (dpeaa)DE-He213 Methylation (dpeaa)DE-He213 Besharat, Zein Mersini verfasserin aut Carai, Andrea verfasserin aut Miele, Evelina verfasserin aut Del Baldo, Giada verfasserin aut Mastronuzzi, Angela verfasserin aut Catanzaro, Giuseppina verfasserin aut Ferretti, Elisabetta verfasserin aut Enthalten in Child's nervous system Berlin : Springer, 1985 37(2020), 3 vom: 11. März, Seite 771-778 (DE-627)254639054 (DE-600)1463024-2 1433-0350 nnns volume:37 year:2020 number:3 day:11 month:03 pages:771-778 https://dx.doi.org/10.1007/s00381-020-04559-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE 44.67 ASE AR 37 2020 3 11 03 771-778 |
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Enthalten in Child's nervous system 37(2020), 3 vom: 11. März, Seite 771-778 volume:37 year:2020 number:3 day:11 month:03 pages:771-778 |
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Patients derived primary cellular models Pediatric low-grade gliomas Molecular characterization Methylation |
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Chiacchiarini, Martina @@aut@@ Besharat, Zein Mersini @@aut@@ Carai, Andrea @@aut@@ Miele, Evelina @@aut@@ Del Baldo, Giada @@aut@@ Mastronuzzi, Angela @@aut@@ Catanzaro, Giuseppina @@aut@@ Ferretti, Elisabetta @@aut@@ |
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A major limitation in the development of treatments for these tumors is the absence of reliable in vitro models that would allow a better understanding of the molecular mechanisms that support their growth. Surgical excision is the primary treatment method and the extent of resection represents one of the strongest prognostic factors. pLGGs that cannot be completely resected are prone to recur and associated with relapses and extensive morbidities, thus remaining a major clinical challenge. Methods We established a protocol to successfully derive primary patient-derived pLGG cells and to fully characterize them from a molecular point of view. Results Primary patients-derived pLGG cells were extensively analyzed in order to confirm their reliability as cellular models. Specifically, we evaluated the growth rate, senescence, and molecular features, such as BRAF mutational status, methylation, and protein expression profile. 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Chiacchiarini, Martina |
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Chiacchiarini, Martina ddc 610 bkl 44.90 bkl 44.67 misc Patients derived primary cellular models misc Pediatric low-grade gliomas misc Molecular characterization misc Methylation Pediatric low-grade gliomas: molecular characterization of patient-derived cellular models |
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610 ASE 44.90 bkl 44.67 bkl Pediatric low-grade gliomas: molecular characterization of patient-derived cellular models Patients derived primary cellular models (dpeaa)DE-He213 Pediatric low-grade gliomas (dpeaa)DE-He213 Molecular characterization (dpeaa)DE-He213 Methylation (dpeaa)DE-He213 |
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ddc 610 bkl 44.90 bkl 44.67 misc Patients derived primary cellular models misc Pediatric low-grade gliomas misc Molecular characterization misc Methylation |
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pediatric low-grade gliomas: molecular characterization of patient-derived cellular models |
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Pediatric low-grade gliomas: molecular characterization of patient-derived cellular models |
| abstract |
Purpose Pediatric low-grade gliomas (pLGGs), the most frequent pediatric brain tumors, include different entities harboring distinct histological and molecular features. A major limitation in the development of treatments for these tumors is the absence of reliable in vitro models that would allow a better understanding of the molecular mechanisms that support their growth. Surgical excision is the primary treatment method and the extent of resection represents one of the strongest prognostic factors. pLGGs that cannot be completely resected are prone to recur and associated with relapses and extensive morbidities, thus remaining a major clinical challenge. Methods We established a protocol to successfully derive primary patient-derived pLGG cells and to fully characterize them from a molecular point of view. Results Primary patients-derived pLGG cells were extensively analyzed in order to confirm their reliability as cellular models. Specifically, we evaluated the growth rate, senescence, and molecular features, such as BRAF mutational status, methylation, and protein expression profile. Conclusion This study extensively describes pLGG primary cellular models in terms of isolation, culture method, and molecular characterization that can be used to investigate pLGG biology. |
| abstractGer |
Purpose Pediatric low-grade gliomas (pLGGs), the most frequent pediatric brain tumors, include different entities harboring distinct histological and molecular features. A major limitation in the development of treatments for these tumors is the absence of reliable in vitro models that would allow a better understanding of the molecular mechanisms that support their growth. Surgical excision is the primary treatment method and the extent of resection represents one of the strongest prognostic factors. pLGGs that cannot be completely resected are prone to recur and associated with relapses and extensive morbidities, thus remaining a major clinical challenge. Methods We established a protocol to successfully derive primary patient-derived pLGG cells and to fully characterize them from a molecular point of view. Results Primary patients-derived pLGG cells were extensively analyzed in order to confirm their reliability as cellular models. Specifically, we evaluated the growth rate, senescence, and molecular features, such as BRAF mutational status, methylation, and protein expression profile. Conclusion This study extensively describes pLGG primary cellular models in terms of isolation, culture method, and molecular characterization that can be used to investigate pLGG biology. |
| abstract_unstemmed |
Purpose Pediatric low-grade gliomas (pLGGs), the most frequent pediatric brain tumors, include different entities harboring distinct histological and molecular features. A major limitation in the development of treatments for these tumors is the absence of reliable in vitro models that would allow a better understanding of the molecular mechanisms that support their growth. Surgical excision is the primary treatment method and the extent of resection represents one of the strongest prognostic factors. pLGGs that cannot be completely resected are prone to recur and associated with relapses and extensive morbidities, thus remaining a major clinical challenge. Methods We established a protocol to successfully derive primary patient-derived pLGG cells and to fully characterize them from a molecular point of view. Results Primary patients-derived pLGG cells were extensively analyzed in order to confirm their reliability as cellular models. Specifically, we evaluated the growth rate, senescence, and molecular features, such as BRAF mutational status, methylation, and protein expression profile. Conclusion This study extensively describes pLGG primary cellular models in terms of isolation, culture method, and molecular characterization that can be used to investigate pLGG biology. |
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Pediatric low-grade gliomas: molecular characterization of patient-derived cellular models |
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Besharat, Zein Mersini Carai, Andrea Miele, Evelina Del Baldo, Giada Mastronuzzi, Angela Catanzaro, Giuseppina Ferretti, Elisabetta |
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| score |
7.4003096 |