An improved and effective protocol for monoconidial isolation of Pyricularia oryzae
Abstract In the present study, a protocol for monoconidial isolation of Pyricularia oryzae from infected field samples has been illustrated. This technique is based on tapping bunch of conidia on 3% water agar medium and then transferring a single germinating conidium in fresh Potato Dextrose Agar (...
Ausführliche Beschreibung
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| Autor*in: |
Al Noman, Md. Abdullah [verfasserIn] Shamsi, Shamim [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2021 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Journal of plant pathology - [Cham] : Springer International Publishing, 1997, 103(2021), 1 vom: 04. Jan., Seite 317-320 |
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| Übergeordnetes Werk: |
volume:103 ; year:2021 ; number:1 ; day:04 ; month:01 ; pages:317-320 |
| Links: |
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| DOI / URN: |
10.1007/s42161-020-00732-x |
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| Katalog-ID: |
SPR043279619 |
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| 245 | 1 | 3 | |a An improved and effective protocol for monoconidial isolation of Pyricularia oryzae |
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| 520 | |a Abstract In the present study, a protocol for monoconidial isolation of Pyricularia oryzae from infected field samples has been illustrated. This technique is based on tapping bunch of conidia on 3% water agar medium and then transferring a single germinating conidium in fresh Potato Dextrose Agar (PDA) medium following 24 hours of incubation. This protocol allows to easily locate single conidial sites, greatly shortened overall time per isolation and remarkably lowered contamination rate during isolation. Moreover, it is more convenient and effective method for the monoconidial isolation of Pyricularia oryzae that can be exploited in less funded laboratories working with this pathogen. | ||
| 650 | 4 | |a Field samples |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Pure culture |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Single spore isolation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Wheat blast |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Shamsi, Shamim |e verfasserin |4 aut | |
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10.1007/s42161-020-00732-x doi (DE-627)SPR043279619 (DE-599)SPRs42161-020-00732-x-e (SPR)s42161-020-00732-x-e DE-627 ger DE-627 rakwb eng 630 580 ASE 630 580 ASE 48.54 bkl Al Noman, Md. Abdullah verfasserin aut An improved and effective protocol for monoconidial isolation of Pyricularia oryzae 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In the present study, a protocol for monoconidial isolation of Pyricularia oryzae from infected field samples has been illustrated. This technique is based on tapping bunch of conidia on 3% water agar medium and then transferring a single germinating conidium in fresh Potato Dextrose Agar (PDA) medium following 24 hours of incubation. This protocol allows to easily locate single conidial sites, greatly shortened overall time per isolation and remarkably lowered contamination rate during isolation. Moreover, it is more convenient and effective method for the monoconidial isolation of Pyricularia oryzae that can be exploited in less funded laboratories working with this pathogen. Field samples (dpeaa)DE-He213 Pure culture (dpeaa)DE-He213 Single spore isolation (dpeaa)DE-He213 Wheat blast (dpeaa)DE-He213 Shamsi, Shamim verfasserin aut Enthalten in Journal of plant pathology [Cham] : Springer International Publishing, 1997 103(2021), 1 vom: 04. Jan., Seite 317-320 (DE-627)504102400 (DE-600)2212051-8 2239-7264 nnns volume:103 year:2021 number:1 day:04 month:01 pages:317-320 https://dx.doi.org/10.1007/s42161-020-00732-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 103 2021 1 04 01 317-320 |
| spelling |
10.1007/s42161-020-00732-x doi (DE-627)SPR043279619 (DE-599)SPRs42161-020-00732-x-e (SPR)s42161-020-00732-x-e DE-627 ger DE-627 rakwb eng 630 580 ASE 630 580 ASE 48.54 bkl Al Noman, Md. Abdullah verfasserin aut An improved and effective protocol for monoconidial isolation of Pyricularia oryzae 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In the present study, a protocol for monoconidial isolation of Pyricularia oryzae from infected field samples has been illustrated. This technique is based on tapping bunch of conidia on 3% water agar medium and then transferring a single germinating conidium in fresh Potato Dextrose Agar (PDA) medium following 24 hours of incubation. This protocol allows to easily locate single conidial sites, greatly shortened overall time per isolation and remarkably lowered contamination rate during isolation. Moreover, it is more convenient and effective method for the monoconidial isolation of Pyricularia oryzae that can be exploited in less funded laboratories working with this pathogen. Field samples (dpeaa)DE-He213 Pure culture (dpeaa)DE-He213 Single spore isolation (dpeaa)DE-He213 Wheat blast (dpeaa)DE-He213 Shamsi, Shamim verfasserin aut Enthalten in Journal of plant pathology [Cham] : Springer International Publishing, 1997 103(2021), 1 vom: 04. Jan., Seite 317-320 (DE-627)504102400 (DE-600)2212051-8 2239-7264 nnns volume:103 year:2021 number:1 day:04 month:01 pages:317-320 https://dx.doi.org/10.1007/s42161-020-00732-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 103 2021 1 04 01 317-320 |
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10.1007/s42161-020-00732-x doi (DE-627)SPR043279619 (DE-599)SPRs42161-020-00732-x-e (SPR)s42161-020-00732-x-e DE-627 ger DE-627 rakwb eng 630 580 ASE 630 580 ASE 48.54 bkl Al Noman, Md. Abdullah verfasserin aut An improved and effective protocol for monoconidial isolation of Pyricularia oryzae 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In the present study, a protocol for monoconidial isolation of Pyricularia oryzae from infected field samples has been illustrated. This technique is based on tapping bunch of conidia on 3% water agar medium and then transferring a single germinating conidium in fresh Potato Dextrose Agar (PDA) medium following 24 hours of incubation. This protocol allows to easily locate single conidial sites, greatly shortened overall time per isolation and remarkably lowered contamination rate during isolation. Moreover, it is more convenient and effective method for the monoconidial isolation of Pyricularia oryzae that can be exploited in less funded laboratories working with this pathogen. Field samples (dpeaa)DE-He213 Pure culture (dpeaa)DE-He213 Single spore isolation (dpeaa)DE-He213 Wheat blast (dpeaa)DE-He213 Shamsi, Shamim verfasserin aut Enthalten in Journal of plant pathology [Cham] : Springer International Publishing, 1997 103(2021), 1 vom: 04. Jan., Seite 317-320 (DE-627)504102400 (DE-600)2212051-8 2239-7264 nnns volume:103 year:2021 number:1 day:04 month:01 pages:317-320 https://dx.doi.org/10.1007/s42161-020-00732-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 103 2021 1 04 01 317-320 |
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10.1007/s42161-020-00732-x doi (DE-627)SPR043279619 (DE-599)SPRs42161-020-00732-x-e (SPR)s42161-020-00732-x-e DE-627 ger DE-627 rakwb eng 630 580 ASE 630 580 ASE 48.54 bkl Al Noman, Md. Abdullah verfasserin aut An improved and effective protocol for monoconidial isolation of Pyricularia oryzae 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In the present study, a protocol for monoconidial isolation of Pyricularia oryzae from infected field samples has been illustrated. This technique is based on tapping bunch of conidia on 3% water agar medium and then transferring a single germinating conidium in fresh Potato Dextrose Agar (PDA) medium following 24 hours of incubation. This protocol allows to easily locate single conidial sites, greatly shortened overall time per isolation and remarkably lowered contamination rate during isolation. Moreover, it is more convenient and effective method for the monoconidial isolation of Pyricularia oryzae that can be exploited in less funded laboratories working with this pathogen. Field samples (dpeaa)DE-He213 Pure culture (dpeaa)DE-He213 Single spore isolation (dpeaa)DE-He213 Wheat blast (dpeaa)DE-He213 Shamsi, Shamim verfasserin aut Enthalten in Journal of plant pathology [Cham] : Springer International Publishing, 1997 103(2021), 1 vom: 04. Jan., Seite 317-320 (DE-627)504102400 (DE-600)2212051-8 2239-7264 nnns volume:103 year:2021 number:1 day:04 month:01 pages:317-320 https://dx.doi.org/10.1007/s42161-020-00732-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 103 2021 1 04 01 317-320 |
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10.1007/s42161-020-00732-x doi (DE-627)SPR043279619 (DE-599)SPRs42161-020-00732-x-e (SPR)s42161-020-00732-x-e DE-627 ger DE-627 rakwb eng 630 580 ASE 630 580 ASE 48.54 bkl Al Noman, Md. Abdullah verfasserin aut An improved and effective protocol for monoconidial isolation of Pyricularia oryzae 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In the present study, a protocol for monoconidial isolation of Pyricularia oryzae from infected field samples has been illustrated. This technique is based on tapping bunch of conidia on 3% water agar medium and then transferring a single germinating conidium in fresh Potato Dextrose Agar (PDA) medium following 24 hours of incubation. This protocol allows to easily locate single conidial sites, greatly shortened overall time per isolation and remarkably lowered contamination rate during isolation. Moreover, it is more convenient and effective method for the monoconidial isolation of Pyricularia oryzae that can be exploited in less funded laboratories working with this pathogen. Field samples (dpeaa)DE-He213 Pure culture (dpeaa)DE-He213 Single spore isolation (dpeaa)DE-He213 Wheat blast (dpeaa)DE-He213 Shamsi, Shamim verfasserin aut Enthalten in Journal of plant pathology [Cham] : Springer International Publishing, 1997 103(2021), 1 vom: 04. Jan., Seite 317-320 (DE-627)504102400 (DE-600)2212051-8 2239-7264 nnns volume:103 year:2021 number:1 day:04 month:01 pages:317-320 https://dx.doi.org/10.1007/s42161-020-00732-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 48.54 ASE AR 103 2021 1 04 01 317-320 |
| language |
English |
| source |
Enthalten in Journal of plant pathology 103(2021), 1 vom: 04. Jan., Seite 317-320 volume:103 year:2021 number:1 day:04 month:01 pages:317-320 |
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Enthalten in Journal of plant pathology 103(2021), 1 vom: 04. Jan., Seite 317-320 volume:103 year:2021 number:1 day:04 month:01 pages:317-320 |
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findex.gbv.de |
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Field samples Pure culture Single spore isolation Wheat blast |
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Journal of plant pathology |
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Al Noman, Md. Abdullah @@aut@@ Shamsi, Shamim @@aut@@ |
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2021-01-04T00:00:00Z |
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Al Noman, Md. Abdullah |
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Al Noman, Md. Abdullah ddc 630 bkl 48.54 misc Field samples misc Pure culture misc Single spore isolation misc Wheat blast An improved and effective protocol for monoconidial isolation of Pyricularia oryzae |
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630 580 ASE 48.54 bkl An improved and effective protocol for monoconidial isolation of Pyricularia oryzae Field samples (dpeaa)DE-He213 Pure culture (dpeaa)DE-He213 Single spore isolation (dpeaa)DE-He213 Wheat blast (dpeaa)DE-He213 |
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ddc 630 bkl 48.54 misc Field samples misc Pure culture misc Single spore isolation misc Wheat blast |
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An improved and effective protocol for monoconidial isolation of Pyricularia oryzae |
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An improved and effective protocol for monoconidial isolation of Pyricularia oryzae |
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improved and effective protocol for monoconidial isolation of pyricularia oryzae |
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An improved and effective protocol for monoconidial isolation of Pyricularia oryzae |
| abstract |
Abstract In the present study, a protocol for monoconidial isolation of Pyricularia oryzae from infected field samples has been illustrated. This technique is based on tapping bunch of conidia on 3% water agar medium and then transferring a single germinating conidium in fresh Potato Dextrose Agar (PDA) medium following 24 hours of incubation. This protocol allows to easily locate single conidial sites, greatly shortened overall time per isolation and remarkably lowered contamination rate during isolation. Moreover, it is more convenient and effective method for the monoconidial isolation of Pyricularia oryzae that can be exploited in less funded laboratories working with this pathogen. |
| abstractGer |
Abstract In the present study, a protocol for monoconidial isolation of Pyricularia oryzae from infected field samples has been illustrated. This technique is based on tapping bunch of conidia on 3% water agar medium and then transferring a single germinating conidium in fresh Potato Dextrose Agar (PDA) medium following 24 hours of incubation. This protocol allows to easily locate single conidial sites, greatly shortened overall time per isolation and remarkably lowered contamination rate during isolation. Moreover, it is more convenient and effective method for the monoconidial isolation of Pyricularia oryzae that can be exploited in less funded laboratories working with this pathogen. |
| abstract_unstemmed |
Abstract In the present study, a protocol for monoconidial isolation of Pyricularia oryzae from infected field samples has been illustrated. This technique is based on tapping bunch of conidia on 3% water agar medium and then transferring a single germinating conidium in fresh Potato Dextrose Agar (PDA) medium following 24 hours of incubation. This protocol allows to easily locate single conidial sites, greatly shortened overall time per isolation and remarkably lowered contamination rate during isolation. Moreover, it is more convenient and effective method for the monoconidial isolation of Pyricularia oryzae that can be exploited in less funded laboratories working with this pathogen. |
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| title_short |
An improved and effective protocol for monoconidial isolation of Pyricularia oryzae |
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https://dx.doi.org/10.1007/s42161-020-00732-x |
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Shamsi, Shamim |
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Shamsi, Shamim |
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| doi_str |
10.1007/s42161-020-00732-x |
| up_date |
2024-07-03T17:41:29.454Z |
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| score |
7.397746 |