[Pt(O,O'-acac)(γ-acac)(DMS)]: Alternative Strategies to Overcome Cisplatin-Induced Side Effects and Resistance in T98G Glioma Cells
Abstract Cisplatin (CDDP) is one of the most effective chemotherapeutic agents, used for the treatment of diverse tumors, including neuroblastoma and glioblastoma. CDDP induces cell death through different apoptotic pathways. Despite its clinical benefits, CDDP causes several side effects and drug r...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Astesana, Valentina [verfasserIn] Faris, Pawan [verfasserIn] Ferrari, Beatrice [verfasserIn] Siciliani, Stella [verfasserIn] Lim, Dmitry [verfasserIn] Biggiogera, Marco [verfasserIn] De Pascali, Sandra Angelica [verfasserIn] Fanizzi, Francesco Paolo [verfasserIn] Roda, Elisa [verfasserIn] Moccia, Francesco [verfasserIn] Bottone, Maria Grazia [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Cellular and molecular neurobiology - Dordrecht : Springer Science + Business Media B.V, 1981, 41(2020), 3 vom: 19. Mai, Seite 563-587 |
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| Übergeordnetes Werk: |
volume:41 ; year:2020 ; number:3 ; day:19 ; month:05 ; pages:563-587 |
| Links: |
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| DOI / URN: |
10.1007/s10571-020-00873-8 |
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| Katalog-ID: |
SPR043358233 |
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| 520 | |a Abstract Cisplatin (CDDP) is one of the most effective chemotherapeutic agents, used for the treatment of diverse tumors, including neuroblastoma and glioblastoma. CDDP induces cell death through different apoptotic pathways. Despite its clinical benefits, CDDP causes several side effects and drug resistance.[Pt(O,O′-acac)(γ-acac)(DMS)], namely PtAcacDMS, a new platinum(II) complex containing two acetylacetonate (acac) and a dimethylsulphide (DMS) in the coordination sphere of metal, has been recently synthesized and showed 100 times higher cytotoxicity than CDDP. Additionally, PtAcacDMS was associated to a decreased neurotoxicity in developing rat central nervous system, also displaying great antitumor and antiangiogenic activity both in vivo and in vitro. Thus, based on the knowledge that several chemotherapeutics induce cancer cell death through an aberrant increase in [$ Ca^{2+} $]i, in the present in vitro study we compared CDDP and PtAcacDMS effects on apoptosis and intracellular $ Ca^{2+} $ dynamics in human glioblastoma T98G cells, applying a battery of complementary techniques, i.e., flow cytometry, immunocytochemistry, electron microscopy, Western blotting, qRT-PCR, and epifluorescent $ Ca^{2+} $ imaging. The results confirmed that (i) platinum compounds may induce cell death through an aberrant increase in [$ Ca^{2+} $]i and (ii) PtAcacDMS exerted stronger cytotoxic effect than CDDP, associated to a larger increase in resting [$ Ca^{2+} $]i. These findings corroborate the use of PtAcacDMS as a promising approach to improve Pt-based chemotherapy against gliomas, either by inducing a chemosensitization or reducing chemoresistance in cell lineages resilient to CDDP treatment. | ||
| 650 | 4 | |a Pt(O,O'-acac)( |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a -acac)(DMS) |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Cisplatin |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Apoptosis |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a T98G glioblastoma cells |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Ca |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a signaling |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Faris, Pawan |e verfasserin |4 aut | |
| 700 | 1 | |a Ferrari, Beatrice |e verfasserin |4 aut | |
| 700 | 1 | |a Siciliani, Stella |e verfasserin |4 aut | |
| 700 | 1 | |a Lim, Dmitry |e verfasserin |4 aut | |
| 700 | 1 | |a Biggiogera, Marco |e verfasserin |4 aut | |
| 700 | 1 | |a De Pascali, Sandra Angelica |e verfasserin |4 aut | |
| 700 | 1 | |a Fanizzi, Francesco Paolo |e verfasserin |4 aut | |
| 700 | 1 | |a Roda, Elisa |e verfasserin |4 aut | |
| 700 | 1 | |a Moccia, Francesco |e verfasserin |4 aut | |
| 700 | 1 | |a Bottone, Maria Grazia |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cellular and molecular neurobiology |d Dordrecht : Springer Science + Business Media B.V, 1981 |g 41(2020), 3 vom: 19. Mai, Seite 563-587 |w (DE-627)306351536 |w (DE-600)1496697-9 |x 1573-6830 |7 nnns |
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10.1007/s10571-020-00873-8 doi (DE-627)SPR043358233 (DE-599)SPRs10571-020-00873-8-e (SPR)s10571-020-00873-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl Astesana, Valentina verfasserin aut [Pt(O,O'-acac)(γ-acac)(DMS)]: Alternative Strategies to Overcome Cisplatin-Induced Side Effects and Resistance in T98G Glioma Cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Cisplatin (CDDP) is one of the most effective chemotherapeutic agents, used for the treatment of diverse tumors, including neuroblastoma and glioblastoma. CDDP induces cell death through different apoptotic pathways. Despite its clinical benefits, CDDP causes several side effects and drug resistance.[Pt(O,O′-acac)(γ-acac)(DMS)], namely PtAcacDMS, a new platinum(II) complex containing two acetylacetonate (acac) and a dimethylsulphide (DMS) in the coordination sphere of metal, has been recently synthesized and showed 100 times higher cytotoxicity than CDDP. Additionally, PtAcacDMS was associated to a decreased neurotoxicity in developing rat central nervous system, also displaying great antitumor and antiangiogenic activity both in vivo and in vitro. Thus, based on the knowledge that several chemotherapeutics induce cancer cell death through an aberrant increase in [$ Ca^{2+} $]i, in the present in vitro study we compared CDDP and PtAcacDMS effects on apoptosis and intracellular $ Ca^{2+} $ dynamics in human glioblastoma T98G cells, applying a battery of complementary techniques, i.e., flow cytometry, immunocytochemistry, electron microscopy, Western blotting, qRT-PCR, and epifluorescent $ Ca^{2+} $ imaging. The results confirmed that (i) platinum compounds may induce cell death through an aberrant increase in [$ Ca^{2+} $]i and (ii) PtAcacDMS exerted stronger cytotoxic effect than CDDP, associated to a larger increase in resting [$ Ca^{2+} $]i. These findings corroborate the use of PtAcacDMS as a promising approach to improve Pt-based chemotherapy against gliomas, either by inducing a chemosensitization or reducing chemoresistance in cell lineages resilient to CDDP treatment. Pt(O,O'-acac)( (dpeaa)DE-He213 -acac)(DMS) (dpeaa)DE-He213 Cisplatin (dpeaa)DE-He213 Apoptosis (dpeaa)DE-He213 T98G glioblastoma cells (dpeaa)DE-He213 Ca (dpeaa)DE-He213 signaling (dpeaa)DE-He213 Faris, Pawan verfasserin aut Ferrari, Beatrice verfasserin aut Siciliani, Stella verfasserin aut Lim, Dmitry verfasserin aut Biggiogera, Marco verfasserin aut De Pascali, Sandra Angelica verfasserin aut Fanizzi, Francesco Paolo verfasserin aut Roda, Elisa verfasserin aut Moccia, Francesco verfasserin aut Bottone, Maria Grazia verfasserin aut Enthalten in Cellular and molecular neurobiology Dordrecht : Springer Science + Business Media B.V, 1981 41(2020), 3 vom: 19. Mai, Seite 563-587 (DE-627)306351536 (DE-600)1496697-9 1573-6830 nnns volume:41 year:2020 number:3 day:19 month:05 pages:563-587 https://dx.doi.org/10.1007/s10571-020-00873-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE AR 41 2020 3 19 05 563-587 |
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10.1007/s10571-020-00873-8 doi (DE-627)SPR043358233 (DE-599)SPRs10571-020-00873-8-e (SPR)s10571-020-00873-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl Astesana, Valentina verfasserin aut [Pt(O,O'-acac)(γ-acac)(DMS)]: Alternative Strategies to Overcome Cisplatin-Induced Side Effects and Resistance in T98G Glioma Cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Cisplatin (CDDP) is one of the most effective chemotherapeutic agents, used for the treatment of diverse tumors, including neuroblastoma and glioblastoma. CDDP induces cell death through different apoptotic pathways. Despite its clinical benefits, CDDP causes several side effects and drug resistance.[Pt(O,O′-acac)(γ-acac)(DMS)], namely PtAcacDMS, a new platinum(II) complex containing two acetylacetonate (acac) and a dimethylsulphide (DMS) in the coordination sphere of metal, has been recently synthesized and showed 100 times higher cytotoxicity than CDDP. Additionally, PtAcacDMS was associated to a decreased neurotoxicity in developing rat central nervous system, also displaying great antitumor and antiangiogenic activity both in vivo and in vitro. Thus, based on the knowledge that several chemotherapeutics induce cancer cell death through an aberrant increase in [$ Ca^{2+} $]i, in the present in vitro study we compared CDDP and PtAcacDMS effects on apoptosis and intracellular $ Ca^{2+} $ dynamics in human glioblastoma T98G cells, applying a battery of complementary techniques, i.e., flow cytometry, immunocytochemistry, electron microscopy, Western blotting, qRT-PCR, and epifluorescent $ Ca^{2+} $ imaging. The results confirmed that (i) platinum compounds may induce cell death through an aberrant increase in [$ Ca^{2+} $]i and (ii) PtAcacDMS exerted stronger cytotoxic effect than CDDP, associated to a larger increase in resting [$ Ca^{2+} $]i. These findings corroborate the use of PtAcacDMS as a promising approach to improve Pt-based chemotherapy against gliomas, either by inducing a chemosensitization or reducing chemoresistance in cell lineages resilient to CDDP treatment. Pt(O,O'-acac)( (dpeaa)DE-He213 -acac)(DMS) (dpeaa)DE-He213 Cisplatin (dpeaa)DE-He213 Apoptosis (dpeaa)DE-He213 T98G glioblastoma cells (dpeaa)DE-He213 Ca (dpeaa)DE-He213 signaling (dpeaa)DE-He213 Faris, Pawan verfasserin aut Ferrari, Beatrice verfasserin aut Siciliani, Stella verfasserin aut Lim, Dmitry verfasserin aut Biggiogera, Marco verfasserin aut De Pascali, Sandra Angelica verfasserin aut Fanizzi, Francesco Paolo verfasserin aut Roda, Elisa verfasserin aut Moccia, Francesco verfasserin aut Bottone, Maria Grazia verfasserin aut Enthalten in Cellular and molecular neurobiology Dordrecht : Springer Science + Business Media B.V, 1981 41(2020), 3 vom: 19. Mai, Seite 563-587 (DE-627)306351536 (DE-600)1496697-9 1573-6830 nnns volume:41 year:2020 number:3 day:19 month:05 pages:563-587 https://dx.doi.org/10.1007/s10571-020-00873-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE AR 41 2020 3 19 05 563-587 |
| allfields_unstemmed |
10.1007/s10571-020-00873-8 doi (DE-627)SPR043358233 (DE-599)SPRs10571-020-00873-8-e (SPR)s10571-020-00873-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl Astesana, Valentina verfasserin aut [Pt(O,O'-acac)(γ-acac)(DMS)]: Alternative Strategies to Overcome Cisplatin-Induced Side Effects and Resistance in T98G Glioma Cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Cisplatin (CDDP) is one of the most effective chemotherapeutic agents, used for the treatment of diverse tumors, including neuroblastoma and glioblastoma. CDDP induces cell death through different apoptotic pathways. Despite its clinical benefits, CDDP causes several side effects and drug resistance.[Pt(O,O′-acac)(γ-acac)(DMS)], namely PtAcacDMS, a new platinum(II) complex containing two acetylacetonate (acac) and a dimethylsulphide (DMS) in the coordination sphere of metal, has been recently synthesized and showed 100 times higher cytotoxicity than CDDP. Additionally, PtAcacDMS was associated to a decreased neurotoxicity in developing rat central nervous system, also displaying great antitumor and antiangiogenic activity both in vivo and in vitro. Thus, based on the knowledge that several chemotherapeutics induce cancer cell death through an aberrant increase in [$ Ca^{2+} $]i, in the present in vitro study we compared CDDP and PtAcacDMS effects on apoptosis and intracellular $ Ca^{2+} $ dynamics in human glioblastoma T98G cells, applying a battery of complementary techniques, i.e., flow cytometry, immunocytochemistry, electron microscopy, Western blotting, qRT-PCR, and epifluorescent $ Ca^{2+} $ imaging. The results confirmed that (i) platinum compounds may induce cell death through an aberrant increase in [$ Ca^{2+} $]i and (ii) PtAcacDMS exerted stronger cytotoxic effect than CDDP, associated to a larger increase in resting [$ Ca^{2+} $]i. These findings corroborate the use of PtAcacDMS as a promising approach to improve Pt-based chemotherapy against gliomas, either by inducing a chemosensitization or reducing chemoresistance in cell lineages resilient to CDDP treatment. Pt(O,O'-acac)( (dpeaa)DE-He213 -acac)(DMS) (dpeaa)DE-He213 Cisplatin (dpeaa)DE-He213 Apoptosis (dpeaa)DE-He213 T98G glioblastoma cells (dpeaa)DE-He213 Ca (dpeaa)DE-He213 signaling (dpeaa)DE-He213 Faris, Pawan verfasserin aut Ferrari, Beatrice verfasserin aut Siciliani, Stella verfasserin aut Lim, Dmitry verfasserin aut Biggiogera, Marco verfasserin aut De Pascali, Sandra Angelica verfasserin aut Fanizzi, Francesco Paolo verfasserin aut Roda, Elisa verfasserin aut Moccia, Francesco verfasserin aut Bottone, Maria Grazia verfasserin aut Enthalten in Cellular and molecular neurobiology Dordrecht : Springer Science + Business Media B.V, 1981 41(2020), 3 vom: 19. Mai, Seite 563-587 (DE-627)306351536 (DE-600)1496697-9 1573-6830 nnns volume:41 year:2020 number:3 day:19 month:05 pages:563-587 https://dx.doi.org/10.1007/s10571-020-00873-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE AR 41 2020 3 19 05 563-587 |
| allfieldsGer |
10.1007/s10571-020-00873-8 doi (DE-627)SPR043358233 (DE-599)SPRs10571-020-00873-8-e (SPR)s10571-020-00873-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl Astesana, Valentina verfasserin aut [Pt(O,O'-acac)(γ-acac)(DMS)]: Alternative Strategies to Overcome Cisplatin-Induced Side Effects and Resistance in T98G Glioma Cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Cisplatin (CDDP) is one of the most effective chemotherapeutic agents, used for the treatment of diverse tumors, including neuroblastoma and glioblastoma. CDDP induces cell death through different apoptotic pathways. Despite its clinical benefits, CDDP causes several side effects and drug resistance.[Pt(O,O′-acac)(γ-acac)(DMS)], namely PtAcacDMS, a new platinum(II) complex containing two acetylacetonate (acac) and a dimethylsulphide (DMS) in the coordination sphere of metal, has been recently synthesized and showed 100 times higher cytotoxicity than CDDP. Additionally, PtAcacDMS was associated to a decreased neurotoxicity in developing rat central nervous system, also displaying great antitumor and antiangiogenic activity both in vivo and in vitro. Thus, based on the knowledge that several chemotherapeutics induce cancer cell death through an aberrant increase in [$ Ca^{2+} $]i, in the present in vitro study we compared CDDP and PtAcacDMS effects on apoptosis and intracellular $ Ca^{2+} $ dynamics in human glioblastoma T98G cells, applying a battery of complementary techniques, i.e., flow cytometry, immunocytochemistry, electron microscopy, Western blotting, qRT-PCR, and epifluorescent $ Ca^{2+} $ imaging. The results confirmed that (i) platinum compounds may induce cell death through an aberrant increase in [$ Ca^{2+} $]i and (ii) PtAcacDMS exerted stronger cytotoxic effect than CDDP, associated to a larger increase in resting [$ Ca^{2+} $]i. These findings corroborate the use of PtAcacDMS as a promising approach to improve Pt-based chemotherapy against gliomas, either by inducing a chemosensitization or reducing chemoresistance in cell lineages resilient to CDDP treatment. Pt(O,O'-acac)( (dpeaa)DE-He213 -acac)(DMS) (dpeaa)DE-He213 Cisplatin (dpeaa)DE-He213 Apoptosis (dpeaa)DE-He213 T98G glioblastoma cells (dpeaa)DE-He213 Ca (dpeaa)DE-He213 signaling (dpeaa)DE-He213 Faris, Pawan verfasserin aut Ferrari, Beatrice verfasserin aut Siciliani, Stella verfasserin aut Lim, Dmitry verfasserin aut Biggiogera, Marco verfasserin aut De Pascali, Sandra Angelica verfasserin aut Fanizzi, Francesco Paolo verfasserin aut Roda, Elisa verfasserin aut Moccia, Francesco verfasserin aut Bottone, Maria Grazia verfasserin aut Enthalten in Cellular and molecular neurobiology Dordrecht : Springer Science + Business Media B.V, 1981 41(2020), 3 vom: 19. Mai, Seite 563-587 (DE-627)306351536 (DE-600)1496697-9 1573-6830 nnns volume:41 year:2020 number:3 day:19 month:05 pages:563-587 https://dx.doi.org/10.1007/s10571-020-00873-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE AR 41 2020 3 19 05 563-587 |
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10.1007/s10571-020-00873-8 doi (DE-627)SPR043358233 (DE-599)SPRs10571-020-00873-8-e (SPR)s10571-020-00873-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.90 bkl Astesana, Valentina verfasserin aut [Pt(O,O'-acac)(γ-acac)(DMS)]: Alternative Strategies to Overcome Cisplatin-Induced Side Effects and Resistance in T98G Glioma Cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Cisplatin (CDDP) is one of the most effective chemotherapeutic agents, used for the treatment of diverse tumors, including neuroblastoma and glioblastoma. CDDP induces cell death through different apoptotic pathways. Despite its clinical benefits, CDDP causes several side effects and drug resistance.[Pt(O,O′-acac)(γ-acac)(DMS)], namely PtAcacDMS, a new platinum(II) complex containing two acetylacetonate (acac) and a dimethylsulphide (DMS) in the coordination sphere of metal, has been recently synthesized and showed 100 times higher cytotoxicity than CDDP. Additionally, PtAcacDMS was associated to a decreased neurotoxicity in developing rat central nervous system, also displaying great antitumor and antiangiogenic activity both in vivo and in vitro. Thus, based on the knowledge that several chemotherapeutics induce cancer cell death through an aberrant increase in [$ Ca^{2+} $]i, in the present in vitro study we compared CDDP and PtAcacDMS effects on apoptosis and intracellular $ Ca^{2+} $ dynamics in human glioblastoma T98G cells, applying a battery of complementary techniques, i.e., flow cytometry, immunocytochemistry, electron microscopy, Western blotting, qRT-PCR, and epifluorescent $ Ca^{2+} $ imaging. The results confirmed that (i) platinum compounds may induce cell death through an aberrant increase in [$ Ca^{2+} $]i and (ii) PtAcacDMS exerted stronger cytotoxic effect than CDDP, associated to a larger increase in resting [$ Ca^{2+} $]i. These findings corroborate the use of PtAcacDMS as a promising approach to improve Pt-based chemotherapy against gliomas, either by inducing a chemosensitization or reducing chemoresistance in cell lineages resilient to CDDP treatment. Pt(O,O'-acac)( (dpeaa)DE-He213 -acac)(DMS) (dpeaa)DE-He213 Cisplatin (dpeaa)DE-He213 Apoptosis (dpeaa)DE-He213 T98G glioblastoma cells (dpeaa)DE-He213 Ca (dpeaa)DE-He213 signaling (dpeaa)DE-He213 Faris, Pawan verfasserin aut Ferrari, Beatrice verfasserin aut Siciliani, Stella verfasserin aut Lim, Dmitry verfasserin aut Biggiogera, Marco verfasserin aut De Pascali, Sandra Angelica verfasserin aut Fanizzi, Francesco Paolo verfasserin aut Roda, Elisa verfasserin aut Moccia, Francesco verfasserin aut Bottone, Maria Grazia verfasserin aut Enthalten in Cellular and molecular neurobiology Dordrecht : Springer Science + Business Media B.V, 1981 41(2020), 3 vom: 19. Mai, Seite 563-587 (DE-627)306351536 (DE-600)1496697-9 1573-6830 nnns volume:41 year:2020 number:3 day:19 month:05 pages:563-587 https://dx.doi.org/10.1007/s10571-020-00873-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.90 ASE AR 41 2020 3 19 05 563-587 |
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Pt(O,O'-acac)( -acac)(DMS) Cisplatin Apoptosis T98G glioblastoma cells Ca signaling |
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Astesana, Valentina @@aut@@ Faris, Pawan @@aut@@ Ferrari, Beatrice @@aut@@ Siciliani, Stella @@aut@@ Lim, Dmitry @@aut@@ Biggiogera, Marco @@aut@@ De Pascali, Sandra Angelica @@aut@@ Fanizzi, Francesco Paolo @@aut@@ Roda, Elisa @@aut@@ Moccia, Francesco @@aut@@ Bottone, Maria Grazia @@aut@@ |
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| author |
Astesana, Valentina |
| spellingShingle |
Astesana, Valentina ddc 610 bkl 44.90 misc Pt(O,O'-acac)( misc -acac)(DMS) misc Cisplatin misc Apoptosis misc T98G glioblastoma cells misc Ca misc signaling [Pt(O,O'-acac)(γ-acac)(DMS)]: Alternative Strategies to Overcome Cisplatin-Induced Side Effects and Resistance in T98G Glioma Cells |
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1573-6830 |
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610 ASE 44.90 bkl [Pt(O,O'-acac)(γ-acac)(DMS)]: Alternative Strategies to Overcome Cisplatin-Induced Side Effects and Resistance in T98G Glioma Cells Pt(O,O'-acac)( (dpeaa)DE-He213 -acac)(DMS) (dpeaa)DE-He213 Cisplatin (dpeaa)DE-He213 Apoptosis (dpeaa)DE-He213 T98G glioblastoma cells (dpeaa)DE-He213 Ca (dpeaa)DE-He213 signaling (dpeaa)DE-He213 |
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ddc 610 bkl 44.90 misc Pt(O,O'-acac)( misc -acac)(DMS) misc Cisplatin misc Apoptosis misc T98G glioblastoma cells misc Ca misc signaling |
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ddc 610 bkl 44.90 misc Pt(O,O'-acac)( misc -acac)(DMS) misc Cisplatin misc Apoptosis misc T98G glioblastoma cells misc Ca misc signaling |
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ddc 610 bkl 44.90 misc Pt(O,O'-acac)( misc -acac)(DMS) misc Cisplatin misc Apoptosis misc T98G glioblastoma cells misc Ca misc signaling |
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[Pt(O,O'-acac)(γ-acac)(DMS)]: Alternative Strategies to Overcome Cisplatin-Induced Side Effects and Resistance in T98G Glioma Cells |
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(DE-627)SPR043358233 (DE-599)SPRs10571-020-00873-8-e (SPR)s10571-020-00873-8-e |
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[Pt(O,O'-acac)(γ-acac)(DMS)]: Alternative Strategies to Overcome Cisplatin-Induced Side Effects and Resistance in T98G Glioma Cells |
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Astesana, Valentina |
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Cellular and molecular neurobiology |
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Cellular and molecular neurobiology |
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Astesana, Valentina Faris, Pawan Ferrari, Beatrice Siciliani, Stella Lim, Dmitry Biggiogera, Marco De Pascali, Sandra Angelica Fanizzi, Francesco Paolo Roda, Elisa Moccia, Francesco Bottone, Maria Grazia |
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Astesana, Valentina |
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10.1007/s10571-020-00873-8 |
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610 |
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verfasserin |
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[pt(o,o'-acac)(γ-acac)(dms)]: alternative strategies to overcome cisplatin-induced side effects and resistance in t98g glioma cells |
| title_auth |
[Pt(O,O'-acac)(γ-acac)(DMS)]: Alternative Strategies to Overcome Cisplatin-Induced Side Effects and Resistance in T98G Glioma Cells |
| abstract |
Abstract Cisplatin (CDDP) is one of the most effective chemotherapeutic agents, used for the treatment of diverse tumors, including neuroblastoma and glioblastoma. CDDP induces cell death through different apoptotic pathways. Despite its clinical benefits, CDDP causes several side effects and drug resistance.[Pt(O,O′-acac)(γ-acac)(DMS)], namely PtAcacDMS, a new platinum(II) complex containing two acetylacetonate (acac) and a dimethylsulphide (DMS) in the coordination sphere of metal, has been recently synthesized and showed 100 times higher cytotoxicity than CDDP. Additionally, PtAcacDMS was associated to a decreased neurotoxicity in developing rat central nervous system, also displaying great antitumor and antiangiogenic activity both in vivo and in vitro. Thus, based on the knowledge that several chemotherapeutics induce cancer cell death through an aberrant increase in [$ Ca^{2+} $]i, in the present in vitro study we compared CDDP and PtAcacDMS effects on apoptosis and intracellular $ Ca^{2+} $ dynamics in human glioblastoma T98G cells, applying a battery of complementary techniques, i.e., flow cytometry, immunocytochemistry, electron microscopy, Western blotting, qRT-PCR, and epifluorescent $ Ca^{2+} $ imaging. The results confirmed that (i) platinum compounds may induce cell death through an aberrant increase in [$ Ca^{2+} $]i and (ii) PtAcacDMS exerted stronger cytotoxic effect than CDDP, associated to a larger increase in resting [$ Ca^{2+} $]i. These findings corroborate the use of PtAcacDMS as a promising approach to improve Pt-based chemotherapy against gliomas, either by inducing a chemosensitization or reducing chemoresistance in cell lineages resilient to CDDP treatment. |
| abstractGer |
Abstract Cisplatin (CDDP) is one of the most effective chemotherapeutic agents, used for the treatment of diverse tumors, including neuroblastoma and glioblastoma. CDDP induces cell death through different apoptotic pathways. Despite its clinical benefits, CDDP causes several side effects and drug resistance.[Pt(O,O′-acac)(γ-acac)(DMS)], namely PtAcacDMS, a new platinum(II) complex containing two acetylacetonate (acac) and a dimethylsulphide (DMS) in the coordination sphere of metal, has been recently synthesized and showed 100 times higher cytotoxicity than CDDP. Additionally, PtAcacDMS was associated to a decreased neurotoxicity in developing rat central nervous system, also displaying great antitumor and antiangiogenic activity both in vivo and in vitro. Thus, based on the knowledge that several chemotherapeutics induce cancer cell death through an aberrant increase in [$ Ca^{2+} $]i, in the present in vitro study we compared CDDP and PtAcacDMS effects on apoptosis and intracellular $ Ca^{2+} $ dynamics in human glioblastoma T98G cells, applying a battery of complementary techniques, i.e., flow cytometry, immunocytochemistry, electron microscopy, Western blotting, qRT-PCR, and epifluorescent $ Ca^{2+} $ imaging. The results confirmed that (i) platinum compounds may induce cell death through an aberrant increase in [$ Ca^{2+} $]i and (ii) PtAcacDMS exerted stronger cytotoxic effect than CDDP, associated to a larger increase in resting [$ Ca^{2+} $]i. These findings corroborate the use of PtAcacDMS as a promising approach to improve Pt-based chemotherapy against gliomas, either by inducing a chemosensitization or reducing chemoresistance in cell lineages resilient to CDDP treatment. |
| abstract_unstemmed |
Abstract Cisplatin (CDDP) is one of the most effective chemotherapeutic agents, used for the treatment of diverse tumors, including neuroblastoma and glioblastoma. CDDP induces cell death through different apoptotic pathways. Despite its clinical benefits, CDDP causes several side effects and drug resistance.[Pt(O,O′-acac)(γ-acac)(DMS)], namely PtAcacDMS, a new platinum(II) complex containing two acetylacetonate (acac) and a dimethylsulphide (DMS) in the coordination sphere of metal, has been recently synthesized and showed 100 times higher cytotoxicity than CDDP. Additionally, PtAcacDMS was associated to a decreased neurotoxicity in developing rat central nervous system, also displaying great antitumor and antiangiogenic activity both in vivo and in vitro. Thus, based on the knowledge that several chemotherapeutics induce cancer cell death through an aberrant increase in [$ Ca^{2+} $]i, in the present in vitro study we compared CDDP and PtAcacDMS effects on apoptosis and intracellular $ Ca^{2+} $ dynamics in human glioblastoma T98G cells, applying a battery of complementary techniques, i.e., flow cytometry, immunocytochemistry, electron microscopy, Western blotting, qRT-PCR, and epifluorescent $ Ca^{2+} $ imaging. The results confirmed that (i) platinum compounds may induce cell death through an aberrant increase in [$ Ca^{2+} $]i and (ii) PtAcacDMS exerted stronger cytotoxic effect than CDDP, associated to a larger increase in resting [$ Ca^{2+} $]i. These findings corroborate the use of PtAcacDMS as a promising approach to improve Pt-based chemotherapy against gliomas, either by inducing a chemosensitization or reducing chemoresistance in cell lineages resilient to CDDP treatment. |
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[Pt(O,O'-acac)(γ-acac)(DMS)]: Alternative Strategies to Overcome Cisplatin-Induced Side Effects and Resistance in T98G Glioma Cells |
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https://dx.doi.org/10.1007/s10571-020-00873-8 |
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Faris, Pawan Ferrari, Beatrice Siciliani, Stella Lim, Dmitry Biggiogera, Marco De Pascali, Sandra Angelica Fanizzi, Francesco Paolo Roda, Elisa Moccia, Francesco Bottone, Maria Grazia |
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