β-Glucosidase production by Trichoderma reesei and Thermoascus aurantiacus by solid state cultivation and application of enzymatic cocktail for saccharification of sugarcane bagasse
Abstract For degradation of sugarcane bagasse (SCB), several enzymes are needed but β-glucosidase is rate limiting in cellulose hydrolysis. Since different microorganisms synthetize characteristic pool of enzymes, mixing extracts produced by different species may increase hydrolytic efficiency due t...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Frassatto, Priscila Aparecida Casciatori [verfasserIn] Casciatori, Fernanda Perpétua [verfasserIn] Thoméo, João Cláudio [verfasserIn] Gomes, Eleni [verfasserIn] Boscolo, Maurício [verfasserIn] da Silva, Roberto [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag GmbH Germany, part of Springer Nature 2020 |
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| Übergeordnetes Werk: |
Enthalten in: Biomass Conversion and Biorefinery - Berlin : Springer, 2011, 11(2020), 2 vom: 25. Jan., Seite 503-513 |
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| Übergeordnetes Werk: |
volume:11 ; year:2020 ; number:2 ; day:25 ; month:01 ; pages:503-513 |
| Links: |
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| DOI / URN: |
10.1007/s13399-020-00608-1 |
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| Katalog-ID: |
SPR043522661 |
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| 520 | |a Abstract For degradation of sugarcane bagasse (SCB), several enzymes are needed but β-glucosidase is rate limiting in cellulose hydrolysis. Since different microorganisms synthetize characteristic pool of enzymes, mixing extracts produced by different species may increase hydrolytic efficiency due to synergism between enzymes in cocktails. This paper reports the study of β-glucosidase production in solid state cultivation (SSC) of two filamentous fungi, thermophilic Thermoascus aurantiacus and mesophilic Trichoderma reesei, and application of the enzymatic extracts on non-pretreated SCB saccharification. Enzyme extract obtained from the thermophilic fungus presented higher β-glucosidase and FPU activities (1.8 U/mL and 10 FPU/mL) than the one from mesophilic (0.2 U/mL and 6 FPU/mL). Optimal SCB hydrolysis was achieved when applying enzymatic cocktail composed of equal volumes of both fungal extracts (3.6 FPU/$ g_{SCB} $, filter paper units per gram SCB, 2.25 FPU/$ g_{SCB} $ provided by extract from T. aurantiacus and 1.35 FPU/$ g_{SCB} $ from T. reesei) at 65 °C. The hydrolysis yield applying the enzyme cocktail, 124 mg total reducing sugars (TRS) per $ g_{SCB} $, was higher than any yield achieved when using the enzyme extracts separately (105 $ mg_{TRS} $/$ g_{SCB} $ using 12.5 FPU per $ g_{SCB} $ from T. aurantiacus at 65 °C; 79 $ mg_{TRS} $/$ g_{SCB} $ using 7.5 FPU per $ g_{SCB} $ from T. reesei at 45 °C). Therefore, the use of the cocktail (3.6 FPU/$ g_{SCB} $) at 65 °C released 18 and 57% more TRS respectively than when extracts from T. aurantiacus or from T. reesei were applied alone, respectively, even reducing enzyme load (FPU) by 70%, corroborating the synergistic effect when both extracts are used together. | ||
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| 650 | 4 | |a Fermentation biotechnology |7 (dpeaa)DE-He213 | |
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| 700 | 1 | |a Thoméo, João Cláudio |e verfasserin |4 aut | |
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| 700 | 1 | |a Boscolo, Maurício |e verfasserin |4 aut | |
| 700 | 1 | |a da Silva, Roberto |e verfasserin |4 aut | |
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10.1007/s13399-020-00608-1 doi (DE-627)SPR043522661 (SPR)s13399-020-00608-1-e DE-627 ger DE-627 rakwb eng 570 ASE Frassatto, Priscila Aparecida Casciatori verfasserin aut β-Glucosidase production by Trichoderma reesei and Thermoascus aurantiacus by solid state cultivation and application of enzymatic cocktail for saccharification of sugarcane bagasse 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2020 Abstract For degradation of sugarcane bagasse (SCB), several enzymes are needed but β-glucosidase is rate limiting in cellulose hydrolysis. Since different microorganisms synthetize characteristic pool of enzymes, mixing extracts produced by different species may increase hydrolytic efficiency due to synergism between enzymes in cocktails. This paper reports the study of β-glucosidase production in solid state cultivation (SSC) of two filamentous fungi, thermophilic Thermoascus aurantiacus and mesophilic Trichoderma reesei, and application of the enzymatic extracts on non-pretreated SCB saccharification. Enzyme extract obtained from the thermophilic fungus presented higher β-glucosidase and FPU activities (1.8 U/mL and 10 FPU/mL) than the one from mesophilic (0.2 U/mL and 6 FPU/mL). Optimal SCB hydrolysis was achieved when applying enzymatic cocktail composed of equal volumes of both fungal extracts (3.6 FPU/$ g_{SCB} $, filter paper units per gram SCB, 2.25 FPU/$ g_{SCB} $ provided by extract from T. aurantiacus and 1.35 FPU/$ g_{SCB} $ from T. reesei) at 65 °C. The hydrolysis yield applying the enzyme cocktail, 124 mg total reducing sugars (TRS) per $ g_{SCB} $, was higher than any yield achieved when using the enzyme extracts separately (105 $ mg_{TRS} $/$ g_{SCB} $ using 12.5 FPU per $ g_{SCB} $ from T. aurantiacus at 65 °C; 79 $ mg_{TRS} $/$ g_{SCB} $ using 7.5 FPU per $ g_{SCB} $ from T. reesei at 45 °C). Therefore, the use of the cocktail (3.6 FPU/$ g_{SCB} $) at 65 °C released 18 and 57% more TRS respectively than when extracts from T. aurantiacus or from T. reesei were applied alone, respectively, even reducing enzyme load (FPU) by 70%, corroborating the synergistic effect when both extracts are used together. Biofuels (dpeaa)DE-He213 Fermentation biotechnology (dpeaa)DE-He213 Fungal growth (dpeaa)DE-He213 Enzymes (dpeaa)DE-He213 Hydrolysis (dpeaa)DE-He213 Casciatori, Fernanda Perpétua verfasserin aut Thoméo, João Cláudio verfasserin aut Gomes, Eleni verfasserin aut Boscolo, Maurício verfasserin aut da Silva, Roberto verfasserin aut Enthalten in Biomass Conversion and Biorefinery Berlin : Springer, 2011 11(2020), 2 vom: 25. Jan., Seite 503-513 (DE-627)645092843 (DE-600)2592298-1 2190-6823 nnns volume:11 year:2020 number:2 day:25 month:01 pages:503-513 https://dx.doi.org/10.1007/s13399-020-00608-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 11 2020 2 25 01 503-513 |
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10.1007/s13399-020-00608-1 doi (DE-627)SPR043522661 (SPR)s13399-020-00608-1-e DE-627 ger DE-627 rakwb eng 570 ASE Frassatto, Priscila Aparecida Casciatori verfasserin aut β-Glucosidase production by Trichoderma reesei and Thermoascus aurantiacus by solid state cultivation and application of enzymatic cocktail for saccharification of sugarcane bagasse 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2020 Abstract For degradation of sugarcane bagasse (SCB), several enzymes are needed but β-glucosidase is rate limiting in cellulose hydrolysis. Since different microorganisms synthetize characteristic pool of enzymes, mixing extracts produced by different species may increase hydrolytic efficiency due to synergism between enzymes in cocktails. This paper reports the study of β-glucosidase production in solid state cultivation (SSC) of two filamentous fungi, thermophilic Thermoascus aurantiacus and mesophilic Trichoderma reesei, and application of the enzymatic extracts on non-pretreated SCB saccharification. Enzyme extract obtained from the thermophilic fungus presented higher β-glucosidase and FPU activities (1.8 U/mL and 10 FPU/mL) than the one from mesophilic (0.2 U/mL and 6 FPU/mL). Optimal SCB hydrolysis was achieved when applying enzymatic cocktail composed of equal volumes of both fungal extracts (3.6 FPU/$ g_{SCB} $, filter paper units per gram SCB, 2.25 FPU/$ g_{SCB} $ provided by extract from T. aurantiacus and 1.35 FPU/$ g_{SCB} $ from T. reesei) at 65 °C. The hydrolysis yield applying the enzyme cocktail, 124 mg total reducing sugars (TRS) per $ g_{SCB} $, was higher than any yield achieved when using the enzyme extracts separately (105 $ mg_{TRS} $/$ g_{SCB} $ using 12.5 FPU per $ g_{SCB} $ from T. aurantiacus at 65 °C; 79 $ mg_{TRS} $/$ g_{SCB} $ using 7.5 FPU per $ g_{SCB} $ from T. reesei at 45 °C). Therefore, the use of the cocktail (3.6 FPU/$ g_{SCB} $) at 65 °C released 18 and 57% more TRS respectively than when extracts from T. aurantiacus or from T. reesei were applied alone, respectively, even reducing enzyme load (FPU) by 70%, corroborating the synergistic effect when both extracts are used together. Biofuels (dpeaa)DE-He213 Fermentation biotechnology (dpeaa)DE-He213 Fungal growth (dpeaa)DE-He213 Enzymes (dpeaa)DE-He213 Hydrolysis (dpeaa)DE-He213 Casciatori, Fernanda Perpétua verfasserin aut Thoméo, João Cláudio verfasserin aut Gomes, Eleni verfasserin aut Boscolo, Maurício verfasserin aut da Silva, Roberto verfasserin aut Enthalten in Biomass Conversion and Biorefinery Berlin : Springer, 2011 11(2020), 2 vom: 25. Jan., Seite 503-513 (DE-627)645092843 (DE-600)2592298-1 2190-6823 nnns volume:11 year:2020 number:2 day:25 month:01 pages:503-513 https://dx.doi.org/10.1007/s13399-020-00608-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 11 2020 2 25 01 503-513 |
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10.1007/s13399-020-00608-1 doi (DE-627)SPR043522661 (SPR)s13399-020-00608-1-e DE-627 ger DE-627 rakwb eng 570 ASE Frassatto, Priscila Aparecida Casciatori verfasserin aut β-Glucosidase production by Trichoderma reesei and Thermoascus aurantiacus by solid state cultivation and application of enzymatic cocktail for saccharification of sugarcane bagasse 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2020 Abstract For degradation of sugarcane bagasse (SCB), several enzymes are needed but β-glucosidase is rate limiting in cellulose hydrolysis. Since different microorganisms synthetize characteristic pool of enzymes, mixing extracts produced by different species may increase hydrolytic efficiency due to synergism between enzymes in cocktails. This paper reports the study of β-glucosidase production in solid state cultivation (SSC) of two filamentous fungi, thermophilic Thermoascus aurantiacus and mesophilic Trichoderma reesei, and application of the enzymatic extracts on non-pretreated SCB saccharification. Enzyme extract obtained from the thermophilic fungus presented higher β-glucosidase and FPU activities (1.8 U/mL and 10 FPU/mL) than the one from mesophilic (0.2 U/mL and 6 FPU/mL). Optimal SCB hydrolysis was achieved when applying enzymatic cocktail composed of equal volumes of both fungal extracts (3.6 FPU/$ g_{SCB} $, filter paper units per gram SCB, 2.25 FPU/$ g_{SCB} $ provided by extract from T. aurantiacus and 1.35 FPU/$ g_{SCB} $ from T. reesei) at 65 °C. The hydrolysis yield applying the enzyme cocktail, 124 mg total reducing sugars (TRS) per $ g_{SCB} $, was higher than any yield achieved when using the enzyme extracts separately (105 $ mg_{TRS} $/$ g_{SCB} $ using 12.5 FPU per $ g_{SCB} $ from T. aurantiacus at 65 °C; 79 $ mg_{TRS} $/$ g_{SCB} $ using 7.5 FPU per $ g_{SCB} $ from T. reesei at 45 °C). Therefore, the use of the cocktail (3.6 FPU/$ g_{SCB} $) at 65 °C released 18 and 57% more TRS respectively than when extracts from T. aurantiacus or from T. reesei were applied alone, respectively, even reducing enzyme load (FPU) by 70%, corroborating the synergistic effect when both extracts are used together. Biofuels (dpeaa)DE-He213 Fermentation biotechnology (dpeaa)DE-He213 Fungal growth (dpeaa)DE-He213 Enzymes (dpeaa)DE-He213 Hydrolysis (dpeaa)DE-He213 Casciatori, Fernanda Perpétua verfasserin aut Thoméo, João Cláudio verfasserin aut Gomes, Eleni verfasserin aut Boscolo, Maurício verfasserin aut da Silva, Roberto verfasserin aut Enthalten in Biomass Conversion and Biorefinery Berlin : Springer, 2011 11(2020), 2 vom: 25. Jan., Seite 503-513 (DE-627)645092843 (DE-600)2592298-1 2190-6823 nnns volume:11 year:2020 number:2 day:25 month:01 pages:503-513 https://dx.doi.org/10.1007/s13399-020-00608-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 11 2020 2 25 01 503-513 |
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10.1007/s13399-020-00608-1 doi (DE-627)SPR043522661 (SPR)s13399-020-00608-1-e DE-627 ger DE-627 rakwb eng 570 ASE Frassatto, Priscila Aparecida Casciatori verfasserin aut β-Glucosidase production by Trichoderma reesei and Thermoascus aurantiacus by solid state cultivation and application of enzymatic cocktail for saccharification of sugarcane bagasse 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2020 Abstract For degradation of sugarcane bagasse (SCB), several enzymes are needed but β-glucosidase is rate limiting in cellulose hydrolysis. Since different microorganisms synthetize characteristic pool of enzymes, mixing extracts produced by different species may increase hydrolytic efficiency due to synergism between enzymes in cocktails. This paper reports the study of β-glucosidase production in solid state cultivation (SSC) of two filamentous fungi, thermophilic Thermoascus aurantiacus and mesophilic Trichoderma reesei, and application of the enzymatic extracts on non-pretreated SCB saccharification. Enzyme extract obtained from the thermophilic fungus presented higher β-glucosidase and FPU activities (1.8 U/mL and 10 FPU/mL) than the one from mesophilic (0.2 U/mL and 6 FPU/mL). Optimal SCB hydrolysis was achieved when applying enzymatic cocktail composed of equal volumes of both fungal extracts (3.6 FPU/$ g_{SCB} $, filter paper units per gram SCB, 2.25 FPU/$ g_{SCB} $ provided by extract from T. aurantiacus and 1.35 FPU/$ g_{SCB} $ from T. reesei) at 65 °C. The hydrolysis yield applying the enzyme cocktail, 124 mg total reducing sugars (TRS) per $ g_{SCB} $, was higher than any yield achieved when using the enzyme extracts separately (105 $ mg_{TRS} $/$ g_{SCB} $ using 12.5 FPU per $ g_{SCB} $ from T. aurantiacus at 65 °C; 79 $ mg_{TRS} $/$ g_{SCB} $ using 7.5 FPU per $ g_{SCB} $ from T. reesei at 45 °C). Therefore, the use of the cocktail (3.6 FPU/$ g_{SCB} $) at 65 °C released 18 and 57% more TRS respectively than when extracts from T. aurantiacus or from T. reesei were applied alone, respectively, even reducing enzyme load (FPU) by 70%, corroborating the synergistic effect when both extracts are used together. Biofuels (dpeaa)DE-He213 Fermentation biotechnology (dpeaa)DE-He213 Fungal growth (dpeaa)DE-He213 Enzymes (dpeaa)DE-He213 Hydrolysis (dpeaa)DE-He213 Casciatori, Fernanda Perpétua verfasserin aut Thoméo, João Cláudio verfasserin aut Gomes, Eleni verfasserin aut Boscolo, Maurício verfasserin aut da Silva, Roberto verfasserin aut Enthalten in Biomass Conversion and Biorefinery Berlin : Springer, 2011 11(2020), 2 vom: 25. Jan., Seite 503-513 (DE-627)645092843 (DE-600)2592298-1 2190-6823 nnns volume:11 year:2020 number:2 day:25 month:01 pages:503-513 https://dx.doi.org/10.1007/s13399-020-00608-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 11 2020 2 25 01 503-513 |
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10.1007/s13399-020-00608-1 doi (DE-627)SPR043522661 (SPR)s13399-020-00608-1-e DE-627 ger DE-627 rakwb eng 570 ASE Frassatto, Priscila Aparecida Casciatori verfasserin aut β-Glucosidase production by Trichoderma reesei and Thermoascus aurantiacus by solid state cultivation and application of enzymatic cocktail for saccharification of sugarcane bagasse 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2020 Abstract For degradation of sugarcane bagasse (SCB), several enzymes are needed but β-glucosidase is rate limiting in cellulose hydrolysis. Since different microorganisms synthetize characteristic pool of enzymes, mixing extracts produced by different species may increase hydrolytic efficiency due to synergism between enzymes in cocktails. This paper reports the study of β-glucosidase production in solid state cultivation (SSC) of two filamentous fungi, thermophilic Thermoascus aurantiacus and mesophilic Trichoderma reesei, and application of the enzymatic extracts on non-pretreated SCB saccharification. Enzyme extract obtained from the thermophilic fungus presented higher β-glucosidase and FPU activities (1.8 U/mL and 10 FPU/mL) than the one from mesophilic (0.2 U/mL and 6 FPU/mL). Optimal SCB hydrolysis was achieved when applying enzymatic cocktail composed of equal volumes of both fungal extracts (3.6 FPU/$ g_{SCB} $, filter paper units per gram SCB, 2.25 FPU/$ g_{SCB} $ provided by extract from T. aurantiacus and 1.35 FPU/$ g_{SCB} $ from T. reesei) at 65 °C. The hydrolysis yield applying the enzyme cocktail, 124 mg total reducing sugars (TRS) per $ g_{SCB} $, was higher than any yield achieved when using the enzyme extracts separately (105 $ mg_{TRS} $/$ g_{SCB} $ using 12.5 FPU per $ g_{SCB} $ from T. aurantiacus at 65 °C; 79 $ mg_{TRS} $/$ g_{SCB} $ using 7.5 FPU per $ g_{SCB} $ from T. reesei at 45 °C). Therefore, the use of the cocktail (3.6 FPU/$ g_{SCB} $) at 65 °C released 18 and 57% more TRS respectively than when extracts from T. aurantiacus or from T. reesei were applied alone, respectively, even reducing enzyme load (FPU) by 70%, corroborating the synergistic effect when both extracts are used together. Biofuels (dpeaa)DE-He213 Fermentation biotechnology (dpeaa)DE-He213 Fungal growth (dpeaa)DE-He213 Enzymes (dpeaa)DE-He213 Hydrolysis (dpeaa)DE-He213 Casciatori, Fernanda Perpétua verfasserin aut Thoméo, João Cláudio verfasserin aut Gomes, Eleni verfasserin aut Boscolo, Maurício verfasserin aut da Silva, Roberto verfasserin aut Enthalten in Biomass Conversion and Biorefinery Berlin : Springer, 2011 11(2020), 2 vom: 25. Jan., Seite 503-513 (DE-627)645092843 (DE-600)2592298-1 2190-6823 nnns volume:11 year:2020 number:2 day:25 month:01 pages:503-513 https://dx.doi.org/10.1007/s13399-020-00608-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 11 2020 2 25 01 503-513 |
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Enthalten in Biomass Conversion and Biorefinery 11(2020), 2 vom: 25. Jan., Seite 503-513 volume:11 year:2020 number:2 day:25 month:01 pages:503-513 |
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Frassatto, Priscila Aparecida Casciatori @@aut@@ Casciatori, Fernanda Perpétua @@aut@@ Thoméo, João Cláudio @@aut@@ Gomes, Eleni @@aut@@ Boscolo, Maurício @@aut@@ da Silva, Roberto @@aut@@ |
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Since different microorganisms synthetize characteristic pool of enzymes, mixing extracts produced by different species may increase hydrolytic efficiency due to synergism between enzymes in cocktails. This paper reports the study of β-glucosidase production in solid state cultivation (SSC) of two filamentous fungi, thermophilic Thermoascus aurantiacus and mesophilic Trichoderma reesei, and application of the enzymatic extracts on non-pretreated SCB saccharification. Enzyme extract obtained from the thermophilic fungus presented higher β-glucosidase and FPU activities (1.8 U/mL and 10 FPU/mL) than the one from mesophilic (0.2 U/mL and 6 FPU/mL). Optimal SCB hydrolysis was achieved when applying enzymatic cocktail composed of equal volumes of both fungal extracts (3.6 FPU/$ g_{SCB} $, filter paper units per gram SCB, 2.25 FPU/$ g_{SCB} $ provided by extract from T. aurantiacus and 1.35 FPU/$ g_{SCB} $ from T. reesei) at 65 °C. The hydrolysis yield applying the enzyme cocktail, 124 mg total reducing sugars (TRS) per $ g_{SCB} $, was higher than any yield achieved when using the enzyme extracts separately (105 $ mg_{TRS} $/$ g_{SCB} $ using 12.5 FPU per $ g_{SCB} $ from T. aurantiacus at 65 °C; 79 $ mg_{TRS} $/$ g_{SCB} $ using 7.5 FPU per $ g_{SCB} $ from T. reesei at 45 °C). Therefore, the use of the cocktail (3.6 FPU/$ g_{SCB} $) at 65 °C released 18 and 57% more TRS respectively than when extracts from T. aurantiacus or from T. reesei were applied alone, respectively, even reducing enzyme load (FPU) by 70%, corroborating the synergistic effect when both extracts are used together.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biofuels</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fermentation biotechnology</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fungal growth</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Enzymes</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Hydrolysis</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Casciatori, Fernanda Perpétua</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Thoméo, João Cláudio</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gomes, Eleni</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Boscolo, Maurício</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">da Silva, Roberto</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Biomass Conversion and Biorefinery</subfield><subfield code="d">Berlin : Springer, 2011</subfield><subfield code="g">11(2020), 2 vom: 25. 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Frassatto, Priscila Aparecida Casciatori |
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Frassatto, Priscila Aparecida Casciatori ddc 570 misc Biofuels misc Fermentation biotechnology misc Fungal growth misc Enzymes misc Hydrolysis β-Glucosidase production by Trichoderma reesei and Thermoascus aurantiacus by solid state cultivation and application of enzymatic cocktail for saccharification of sugarcane bagasse |
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570 ASE β-Glucosidase production by Trichoderma reesei and Thermoascus aurantiacus by solid state cultivation and application of enzymatic cocktail for saccharification of sugarcane bagasse Biofuels (dpeaa)DE-He213 Fermentation biotechnology (dpeaa)DE-He213 Fungal growth (dpeaa)DE-He213 Enzymes (dpeaa)DE-He213 Hydrolysis (dpeaa)DE-He213 |
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β-Glucosidase production by Trichoderma reesei and Thermoascus aurantiacus by solid state cultivation and application of enzymatic cocktail for saccharification of sugarcane bagasse |
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β-Glucosidase production by Trichoderma reesei and Thermoascus aurantiacus by solid state cultivation and application of enzymatic cocktail for saccharification of sugarcane bagasse |
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Frassatto, Priscila Aparecida Casciatori Casciatori, Fernanda Perpétua Thoméo, João Cláudio Gomes, Eleni Boscolo, Maurício da Silva, Roberto |
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Frassatto, Priscila Aparecida Casciatori |
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10.1007/s13399-020-00608-1 |
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β-glucosidase production by trichoderma reesei and thermoascus aurantiacus by solid state cultivation and application of enzymatic cocktail for saccharification of sugarcane bagasse |
| title_auth |
β-Glucosidase production by Trichoderma reesei and Thermoascus aurantiacus by solid state cultivation and application of enzymatic cocktail for saccharification of sugarcane bagasse |
| abstract |
Abstract For degradation of sugarcane bagasse (SCB), several enzymes are needed but β-glucosidase is rate limiting in cellulose hydrolysis. Since different microorganisms synthetize characteristic pool of enzymes, mixing extracts produced by different species may increase hydrolytic efficiency due to synergism between enzymes in cocktails. This paper reports the study of β-glucosidase production in solid state cultivation (SSC) of two filamentous fungi, thermophilic Thermoascus aurantiacus and mesophilic Trichoderma reesei, and application of the enzymatic extracts on non-pretreated SCB saccharification. Enzyme extract obtained from the thermophilic fungus presented higher β-glucosidase and FPU activities (1.8 U/mL and 10 FPU/mL) than the one from mesophilic (0.2 U/mL and 6 FPU/mL). Optimal SCB hydrolysis was achieved when applying enzymatic cocktail composed of equal volumes of both fungal extracts (3.6 FPU/$ g_{SCB} $, filter paper units per gram SCB, 2.25 FPU/$ g_{SCB} $ provided by extract from T. aurantiacus and 1.35 FPU/$ g_{SCB} $ from T. reesei) at 65 °C. The hydrolysis yield applying the enzyme cocktail, 124 mg total reducing sugars (TRS) per $ g_{SCB} $, was higher than any yield achieved when using the enzyme extracts separately (105 $ mg_{TRS} $/$ g_{SCB} $ using 12.5 FPU per $ g_{SCB} $ from T. aurantiacus at 65 °C; 79 $ mg_{TRS} $/$ g_{SCB} $ using 7.5 FPU per $ g_{SCB} $ from T. reesei at 45 °C). Therefore, the use of the cocktail (3.6 FPU/$ g_{SCB} $) at 65 °C released 18 and 57% more TRS respectively than when extracts from T. aurantiacus or from T. reesei were applied alone, respectively, even reducing enzyme load (FPU) by 70%, corroborating the synergistic effect when both extracts are used together. © Springer-Verlag GmbH Germany, part of Springer Nature 2020 |
| abstractGer |
Abstract For degradation of sugarcane bagasse (SCB), several enzymes are needed but β-glucosidase is rate limiting in cellulose hydrolysis. Since different microorganisms synthetize characteristic pool of enzymes, mixing extracts produced by different species may increase hydrolytic efficiency due to synergism between enzymes in cocktails. This paper reports the study of β-glucosidase production in solid state cultivation (SSC) of two filamentous fungi, thermophilic Thermoascus aurantiacus and mesophilic Trichoderma reesei, and application of the enzymatic extracts on non-pretreated SCB saccharification. Enzyme extract obtained from the thermophilic fungus presented higher β-glucosidase and FPU activities (1.8 U/mL and 10 FPU/mL) than the one from mesophilic (0.2 U/mL and 6 FPU/mL). Optimal SCB hydrolysis was achieved when applying enzymatic cocktail composed of equal volumes of both fungal extracts (3.6 FPU/$ g_{SCB} $, filter paper units per gram SCB, 2.25 FPU/$ g_{SCB} $ provided by extract from T. aurantiacus and 1.35 FPU/$ g_{SCB} $ from T. reesei) at 65 °C. The hydrolysis yield applying the enzyme cocktail, 124 mg total reducing sugars (TRS) per $ g_{SCB} $, was higher than any yield achieved when using the enzyme extracts separately (105 $ mg_{TRS} $/$ g_{SCB} $ using 12.5 FPU per $ g_{SCB} $ from T. aurantiacus at 65 °C; 79 $ mg_{TRS} $/$ g_{SCB} $ using 7.5 FPU per $ g_{SCB} $ from T. reesei at 45 °C). Therefore, the use of the cocktail (3.6 FPU/$ g_{SCB} $) at 65 °C released 18 and 57% more TRS respectively than when extracts from T. aurantiacus or from T. reesei were applied alone, respectively, even reducing enzyme load (FPU) by 70%, corroborating the synergistic effect when both extracts are used together. © Springer-Verlag GmbH Germany, part of Springer Nature 2020 |
| abstract_unstemmed |
Abstract For degradation of sugarcane bagasse (SCB), several enzymes are needed but β-glucosidase is rate limiting in cellulose hydrolysis. Since different microorganisms synthetize characteristic pool of enzymes, mixing extracts produced by different species may increase hydrolytic efficiency due to synergism between enzymes in cocktails. This paper reports the study of β-glucosidase production in solid state cultivation (SSC) of two filamentous fungi, thermophilic Thermoascus aurantiacus and mesophilic Trichoderma reesei, and application of the enzymatic extracts on non-pretreated SCB saccharification. Enzyme extract obtained from the thermophilic fungus presented higher β-glucosidase and FPU activities (1.8 U/mL and 10 FPU/mL) than the one from mesophilic (0.2 U/mL and 6 FPU/mL). Optimal SCB hydrolysis was achieved when applying enzymatic cocktail composed of equal volumes of both fungal extracts (3.6 FPU/$ g_{SCB} $, filter paper units per gram SCB, 2.25 FPU/$ g_{SCB} $ provided by extract from T. aurantiacus and 1.35 FPU/$ g_{SCB} $ from T. reesei) at 65 °C. The hydrolysis yield applying the enzyme cocktail, 124 mg total reducing sugars (TRS) per $ g_{SCB} $, was higher than any yield achieved when using the enzyme extracts separately (105 $ mg_{TRS} $/$ g_{SCB} $ using 12.5 FPU per $ g_{SCB} $ from T. aurantiacus at 65 °C; 79 $ mg_{TRS} $/$ g_{SCB} $ using 7.5 FPU per $ g_{SCB} $ from T. reesei at 45 °C). Therefore, the use of the cocktail (3.6 FPU/$ g_{SCB} $) at 65 °C released 18 and 57% more TRS respectively than when extracts from T. aurantiacus or from T. reesei were applied alone, respectively, even reducing enzyme load (FPU) by 70%, corroborating the synergistic effect when both extracts are used together. © Springer-Verlag GmbH Germany, part of Springer Nature 2020 |
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β-Glucosidase production by Trichoderma reesei and Thermoascus aurantiacus by solid state cultivation and application of enzymatic cocktail for saccharification of sugarcane bagasse |
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| score |
7.3992996 |