Expression of p27 and p16 and their clinical significance in gastric cancer

Background Deregulated expression of cell cycle regulators p27 and p16 is associated with cancer progression. $ p27^{kip1} $ and $ p16^{INKa} $ are a cyclin dependent kinase inhibitor whose major target is the cyclinE/CDK2 and cyclinD/CDK4/6 complex, respectively, that governs cell cycle transition...
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Autor*in:	Kumari, S. [verfasserIn] Kumar, P. [verfasserIn] Kumar, M. [verfasserIn] Singh, S. [verfasserIn] Narayan, G. [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	Gastric cancer p27 p16 Expression profile Diagnostic biomarker

Übergeordnetes Werk:	Enthalten in: Revista de oncología - Barcelona : Doyma, 2000, 23(2020), 4 vom: 02. Sept., Seite 856-865
Übergeordnetes Werk:	volume:23 ; year:2020 ; number:4 ; day:02 ; month:09 ; pages:856-865

Links:	Volltext

DOI / URN:	10.1007/s12094-020-02479-4

Katalog-ID:	SPR043559670

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520			\|a Background Deregulated expression of cell cycle regulators p27 and p16 is associated with cancer progression. $ p27^{kip1} $ and $ p16^{INKa} $ are a cyclin dependent kinase inhibitor whose major target is the cyclinE/CDK2 and cyclinD/CDK4/6 complex, respectively, that governs cell cycle transition from late G1 to S phase. Methods We recruited biopsies of a total of 84 subjects including 72 primary tumor biopsies from histopathologically proven gastric carcinoma, 8 adjacent controls and 12 independent controls. We used gastric cancer cell line, AGS, for validation of our data. Expression profiling at transcript level was done by semi-quantitative RT-PCR and at proteome level by immunohistochemistry and immunofluorescence. Receiver operator characteristics analysis was done for determining the diagnostic utility of p27 and p16 with respect to the sensitivity and specificity. Results We demonstrate that p27 and p16 are frequently over expressed in early stages of gastric carcinoma. Our semi-quantitative data show a significant upregulation of p27 (Mean ± SEM, 0.4771 ± 0.0895; p = 0.0001) and p16 (Mean ± SEM, 0.4676 ± 0.04305; p = 0.0001) at mRNA level. Concordant to semi-quantitative data, immunohistochemistry data also showed a significant upregulation of p27 (Mean ± SEM, 196.4 ± 10.84; p < 0.0001) and p16 (Mean ± SEM, 100.4 ± 23.71; p < 0.0001) at protein level. Conclusions The present study showed that the significant upregulation of p27 and p16 were associated with early events in gastric carcinogenesis. Our data suggests that clinical correlation of these differentially expressed genes may be useful as diagnostic biomarkers for early detection of gastric carcinoma and promising therapeutics target for GC patients.
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spelling	10.1007/s12094-020-02479-4 doi (DE-627)SPR043559670 (DE-599)SPRs12094-020-02479-4-e (SPR)s12094-020-02479-4-e DE-627 ger DE-627 rakwb eng 610 ASE Kumari, S. verfasserin aut Expression of p27 and p16 and their clinical significance in gastric cancer 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Deregulated expression of cell cycle regulators p27 and p16 is associated with cancer progression. $ p27^{kip1} $ and $ p16^{INKa} $ are a cyclin dependent kinase inhibitor whose major target is the cyclinE/CDK2 and cyclinD/CDK4/6 complex, respectively, that governs cell cycle transition from late G1 to S phase. Methods We recruited biopsies of a total of 84 subjects including 72 primary tumor biopsies from histopathologically proven gastric carcinoma, 8 adjacent controls and 12 independent controls. We used gastric cancer cell line, AGS, for validation of our data. Expression profiling at transcript level was done by semi-quantitative RT-PCR and at proteome level by immunohistochemistry and immunofluorescence. Receiver operator characteristics analysis was done for determining the diagnostic utility of p27 and p16 with respect to the sensitivity and specificity. Results We demonstrate that p27 and p16 are frequently over expressed in early stages of gastric carcinoma. Our semi-quantitative data show a significant upregulation of p27 (Mean ± SEM, 0.4771 ± 0.0895; p = 0.0001) and p16 (Mean ± SEM, 0.4676 ± 0.04305; p = 0.0001) at mRNA level. Concordant to semi-quantitative data, immunohistochemistry data also showed a significant upregulation of p27 (Mean ± SEM, 196.4 ± 10.84; p < 0.0001) and p16 (Mean ± SEM, 100.4 ± 23.71; p < 0.0001) at protein level. Conclusions The present study showed that the significant upregulation of p27 and p16 were associated with early events in gastric carcinogenesis. Our data suggests that clinical correlation of these differentially expressed genes may be useful as diagnostic biomarkers for early detection of gastric carcinoma and promising therapeutics target for GC patients. Gastric cancer (dpeaa)DE-He213 p27 (dpeaa)DE-He213 p16 (dpeaa)DE-He213 Expression profile (dpeaa)DE-He213 Diagnostic biomarker (dpeaa)DE-He213 Kumar, P. verfasserin aut Kumar, M. verfasserin aut Singh, S. verfasserin aut Narayan, G. verfasserin aut Enthalten in Revista de oncología Barcelona : Doyma, 2000 23(2020), 4 vom: 02. Sept., Seite 856-865 (DE-627)385985452 (DE-600)2143451-7 1578-195X nnns volume:23 year:2020 number:4 day:02 month:09 pages:856-865 https://dx.doi.org/10.1007/s12094-020-02479-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_120 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 AR 23 2020 4 02 09 856-865
allfields_unstemmed	10.1007/s12094-020-02479-4 doi (DE-627)SPR043559670 (DE-599)SPRs12094-020-02479-4-e (SPR)s12094-020-02479-4-e DE-627 ger DE-627 rakwb eng 610 ASE Kumari, S. verfasserin aut Expression of p27 and p16 and their clinical significance in gastric cancer 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Deregulated expression of cell cycle regulators p27 and p16 is associated with cancer progression. $ p27^{kip1} $ and $ p16^{INKa} $ are a cyclin dependent kinase inhibitor whose major target is the cyclinE/CDK2 and cyclinD/CDK4/6 complex, respectively, that governs cell cycle transition from late G1 to S phase. Methods We recruited biopsies of a total of 84 subjects including 72 primary tumor biopsies from histopathologically proven gastric carcinoma, 8 adjacent controls and 12 independent controls. We used gastric cancer cell line, AGS, for validation of our data. Expression profiling at transcript level was done by semi-quantitative RT-PCR and at proteome level by immunohistochemistry and immunofluorescence. Receiver operator characteristics analysis was done for determining the diagnostic utility of p27 and p16 with respect to the sensitivity and specificity. Results We demonstrate that p27 and p16 are frequently over expressed in early stages of gastric carcinoma. Our semi-quantitative data show a significant upregulation of p27 (Mean ± SEM, 0.4771 ± 0.0895; p = 0.0001) and p16 (Mean ± SEM, 0.4676 ± 0.04305; p = 0.0001) at mRNA level. Concordant to semi-quantitative data, immunohistochemistry data also showed a significant upregulation of p27 (Mean ± SEM, 196.4 ± 10.84; p < 0.0001) and p16 (Mean ± SEM, 100.4 ± 23.71; p < 0.0001) at protein level. Conclusions The present study showed that the significant upregulation of p27 and p16 were associated with early events in gastric carcinogenesis. Our data suggests that clinical correlation of these differentially expressed genes may be useful as diagnostic biomarkers for early detection of gastric carcinoma and promising therapeutics target for GC patients. Gastric cancer (dpeaa)DE-He213 p27 (dpeaa)DE-He213 p16 (dpeaa)DE-He213 Expression profile (dpeaa)DE-He213 Diagnostic biomarker (dpeaa)DE-He213 Kumar, P. verfasserin aut Kumar, M. verfasserin aut Singh, S. verfasserin aut Narayan, G. verfasserin aut Enthalten in Revista de oncología Barcelona : Doyma, 2000 23(2020), 4 vom: 02. Sept., Seite 856-865 (DE-627)385985452 (DE-600)2143451-7 1578-195X nnns volume:23 year:2020 number:4 day:02 month:09 pages:856-865 https://dx.doi.org/10.1007/s12094-020-02479-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_120 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 AR 23 2020 4 02 09 856-865
allfieldsGer	10.1007/s12094-020-02479-4 doi (DE-627)SPR043559670 (DE-599)SPRs12094-020-02479-4-e (SPR)s12094-020-02479-4-e DE-627 ger DE-627 rakwb eng 610 ASE Kumari, S. verfasserin aut Expression of p27 and p16 and their clinical significance in gastric cancer 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Deregulated expression of cell cycle regulators p27 and p16 is associated with cancer progression. $ p27^{kip1} $ and $ p16^{INKa} $ are a cyclin dependent kinase inhibitor whose major target is the cyclinE/CDK2 and cyclinD/CDK4/6 complex, respectively, that governs cell cycle transition from late G1 to S phase. Methods We recruited biopsies of a total of 84 subjects including 72 primary tumor biopsies from histopathologically proven gastric carcinoma, 8 adjacent controls and 12 independent controls. We used gastric cancer cell line, AGS, for validation of our data. Expression profiling at transcript level was done by semi-quantitative RT-PCR and at proteome level by immunohistochemistry and immunofluorescence. Receiver operator characteristics analysis was done for determining the diagnostic utility of p27 and p16 with respect to the sensitivity and specificity. Results We demonstrate that p27 and p16 are frequently over expressed in early stages of gastric carcinoma. Our semi-quantitative data show a significant upregulation of p27 (Mean ± SEM, 0.4771 ± 0.0895; p = 0.0001) and p16 (Mean ± SEM, 0.4676 ± 0.04305; p = 0.0001) at mRNA level. Concordant to semi-quantitative data, immunohistochemistry data also showed a significant upregulation of p27 (Mean ± SEM, 196.4 ± 10.84; p < 0.0001) and p16 (Mean ± SEM, 100.4 ± 23.71; p < 0.0001) at protein level. Conclusions The present study showed that the significant upregulation of p27 and p16 were associated with early events in gastric carcinogenesis. Our data suggests that clinical correlation of these differentially expressed genes may be useful as diagnostic biomarkers for early detection of gastric carcinoma and promising therapeutics target for GC patients. Gastric cancer (dpeaa)DE-He213 p27 (dpeaa)DE-He213 p16 (dpeaa)DE-He213 Expression profile (dpeaa)DE-He213 Diagnostic biomarker (dpeaa)DE-He213 Kumar, P. verfasserin aut Kumar, M. verfasserin aut Singh, S. verfasserin aut Narayan, G. verfasserin aut Enthalten in Revista de oncología Barcelona : Doyma, 2000 23(2020), 4 vom: 02. Sept., Seite 856-865 (DE-627)385985452 (DE-600)2143451-7 1578-195X nnns volume:23 year:2020 number:4 day:02 month:09 pages:856-865 https://dx.doi.org/10.1007/s12094-020-02479-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_120 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 AR 23 2020 4 02 09 856-865
allfieldsSound	10.1007/s12094-020-02479-4 doi (DE-627)SPR043559670 (DE-599)SPRs12094-020-02479-4-e (SPR)s12094-020-02479-4-e DE-627 ger DE-627 rakwb eng 610 ASE Kumari, S. verfasserin aut Expression of p27 and p16 and their clinical significance in gastric cancer 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Deregulated expression of cell cycle regulators p27 and p16 is associated with cancer progression. $ p27^{kip1} $ and $ p16^{INKa} $ are a cyclin dependent kinase inhibitor whose major target is the cyclinE/CDK2 and cyclinD/CDK4/6 complex, respectively, that governs cell cycle transition from late G1 to S phase. Methods We recruited biopsies of a total of 84 subjects including 72 primary tumor biopsies from histopathologically proven gastric carcinoma, 8 adjacent controls and 12 independent controls. We used gastric cancer cell line, AGS, for validation of our data. Expression profiling at transcript level was done by semi-quantitative RT-PCR and at proteome level by immunohistochemistry and immunofluorescence. Receiver operator characteristics analysis was done for determining the diagnostic utility of p27 and p16 with respect to the sensitivity and specificity. Results We demonstrate that p27 and p16 are frequently over expressed in early stages of gastric carcinoma. Our semi-quantitative data show a significant upregulation of p27 (Mean ± SEM, 0.4771 ± 0.0895; p = 0.0001) and p16 (Mean ± SEM, 0.4676 ± 0.04305; p = 0.0001) at mRNA level. Concordant to semi-quantitative data, immunohistochemistry data also showed a significant upregulation of p27 (Mean ± SEM, 196.4 ± 10.84; p < 0.0001) and p16 (Mean ± SEM, 100.4 ± 23.71; p < 0.0001) at protein level. Conclusions The present study showed that the significant upregulation of p27 and p16 were associated with early events in gastric carcinogenesis. Our data suggests that clinical correlation of these differentially expressed genes may be useful as diagnostic biomarkers for early detection of gastric carcinoma and promising therapeutics target for GC patients. Gastric cancer (dpeaa)DE-He213 p27 (dpeaa)DE-He213 p16 (dpeaa)DE-He213 Expression profile (dpeaa)DE-He213 Diagnostic biomarker (dpeaa)DE-He213 Kumar, P. verfasserin aut Kumar, M. verfasserin aut Singh, S. verfasserin aut Narayan, G. verfasserin aut Enthalten in Revista de oncología Barcelona : Doyma, 2000 23(2020), 4 vom: 02. Sept., Seite 856-865 (DE-627)385985452 (DE-600)2143451-7 1578-195X nnns volume:23 year:2020 number:4 day:02 month:09 pages:856-865 https://dx.doi.org/10.1007/s12094-020-02479-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_120 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 AR 23 2020 4 02 09 856-865
language	English
source	Enthalten in Revista de oncología 23(2020), 4 vom: 02. Sept., Seite 856-865 volume:23 year:2020 number:4 day:02 month:09 pages:856-865
sourceStr	Enthalten in Revista de oncología 23(2020), 4 vom: 02. Sept., Seite 856-865 volume:23 year:2020 number:4 day:02 month:09 pages:856-865
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Gastric cancer p27 p16 Expression profile Diagnostic biomarker
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Methods We recruited biopsies of a total of 84 subjects including 72 primary tumor biopsies from histopathologically proven gastric carcinoma, 8 adjacent controls and 12 independent controls. We used gastric cancer cell line, AGS, for validation of our data. Expression profiling at transcript level was done by semi-quantitative RT-PCR and at proteome level by immunohistochemistry and immunofluorescence. Receiver operator characteristics analysis was done for determining the diagnostic utility of p27 and p16 with respect to the sensitivity and specificity. Results We demonstrate that p27 and p16 are frequently over expressed in early stages of gastric carcinoma. Our semi-quantitative data show a significant upregulation of p27 (Mean ± SEM, 0.4771 ± 0.0895; p = 0.0001) and p16 (Mean ± SEM, 0.4676 ± 0.04305; p = 0.0001) at mRNA level. 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author	Kumari, S.
spellingShingle	Kumari, S. ddc 610 misc Gastric cancer misc p27 misc p16 misc Expression profile misc Diagnostic biomarker Expression of p27 and p16 and their clinical significance in gastric cancer
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topic_title	610 ASE Expression of p27 and p16 and their clinical significance in gastric cancer Gastric cancer (dpeaa)DE-He213 p27 (dpeaa)DE-He213 p16 (dpeaa)DE-He213 Expression profile (dpeaa)DE-He213 Diagnostic biomarker (dpeaa)DE-He213
topic	ddc 610 misc Gastric cancer misc p27 misc p16 misc Expression profile misc Diagnostic biomarker
topic_unstemmed	ddc 610 misc Gastric cancer misc p27 misc p16 misc Expression profile misc Diagnostic biomarker
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title	Expression of p27 and p16 and their clinical significance in gastric cancer
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title_full	Expression of p27 and p16 and their clinical significance in gastric cancer
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author_browse	Kumari, S. Kumar, P. Kumar, M. Singh, S. Narayan, G.
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title_sort	expression of p27 and p16 and their clinical significance in gastric cancer
title_auth	Expression of p27 and p16 and their clinical significance in gastric cancer
abstract	Background Deregulated expression of cell cycle regulators p27 and p16 is associated with cancer progression. $ p27^{kip1} $ and $ p16^{INKa} $ are a cyclin dependent kinase inhibitor whose major target is the cyclinE/CDK2 and cyclinD/CDK4/6 complex, respectively, that governs cell cycle transition from late G1 to S phase. Methods We recruited biopsies of a total of 84 subjects including 72 primary tumor biopsies from histopathologically proven gastric carcinoma, 8 adjacent controls and 12 independent controls. We used gastric cancer cell line, AGS, for validation of our data. Expression profiling at transcript level was done by semi-quantitative RT-PCR and at proteome level by immunohistochemistry and immunofluorescence. Receiver operator characteristics analysis was done for determining the diagnostic utility of p27 and p16 with respect to the sensitivity and specificity. Results We demonstrate that p27 and p16 are frequently over expressed in early stages of gastric carcinoma. Our semi-quantitative data show a significant upregulation of p27 (Mean ± SEM, 0.4771 ± 0.0895; p = 0.0001) and p16 (Mean ± SEM, 0.4676 ± 0.04305; p = 0.0001) at mRNA level. Concordant to semi-quantitative data, immunohistochemistry data also showed a significant upregulation of p27 (Mean ± SEM, 196.4 ± 10.84; p < 0.0001) and p16 (Mean ± SEM, 100.4 ± 23.71; p < 0.0001) at protein level. Conclusions The present study showed that the significant upregulation of p27 and p16 were associated with early events in gastric carcinogenesis. Our data suggests that clinical correlation of these differentially expressed genes may be useful as diagnostic biomarkers for early detection of gastric carcinoma and promising therapeutics target for GC patients.
abstractGer	Background Deregulated expression of cell cycle regulators p27 and p16 is associated with cancer progression. $ p27^{kip1} $ and $ p16^{INKa} $ are a cyclin dependent kinase inhibitor whose major target is the cyclinE/CDK2 and cyclinD/CDK4/6 complex, respectively, that governs cell cycle transition from late G1 to S phase. Methods We recruited biopsies of a total of 84 subjects including 72 primary tumor biopsies from histopathologically proven gastric carcinoma, 8 adjacent controls and 12 independent controls. We used gastric cancer cell line, AGS, for validation of our data. Expression profiling at transcript level was done by semi-quantitative RT-PCR and at proteome level by immunohistochemistry and immunofluorescence. Receiver operator characteristics analysis was done for determining the diagnostic utility of p27 and p16 with respect to the sensitivity and specificity. Results We demonstrate that p27 and p16 are frequently over expressed in early stages of gastric carcinoma. Our semi-quantitative data show a significant upregulation of p27 (Mean ± SEM, 0.4771 ± 0.0895; p = 0.0001) and p16 (Mean ± SEM, 0.4676 ± 0.04305; p = 0.0001) at mRNA level. Concordant to semi-quantitative data, immunohistochemistry data also showed a significant upregulation of p27 (Mean ± SEM, 196.4 ± 10.84; p < 0.0001) and p16 (Mean ± SEM, 100.4 ± 23.71; p < 0.0001) at protein level. Conclusions The present study showed that the significant upregulation of p27 and p16 were associated with early events in gastric carcinogenesis. Our data suggests that clinical correlation of these differentially expressed genes may be useful as diagnostic biomarkers for early detection of gastric carcinoma and promising therapeutics target for GC patients.
abstract_unstemmed	Background Deregulated expression of cell cycle regulators p27 and p16 is associated with cancer progression. $ p27^{kip1} $ and $ p16^{INKa} $ are a cyclin dependent kinase inhibitor whose major target is the cyclinE/CDK2 and cyclinD/CDK4/6 complex, respectively, that governs cell cycle transition from late G1 to S phase. Methods We recruited biopsies of a total of 84 subjects including 72 primary tumor biopsies from histopathologically proven gastric carcinoma, 8 adjacent controls and 12 independent controls. We used gastric cancer cell line, AGS, for validation of our data. Expression profiling at transcript level was done by semi-quantitative RT-PCR and at proteome level by immunohistochemistry and immunofluorescence. Receiver operator characteristics analysis was done for determining the diagnostic utility of p27 and p16 with respect to the sensitivity and specificity. Results We demonstrate that p27 and p16 are frequently over expressed in early stages of gastric carcinoma. Our semi-quantitative data show a significant upregulation of p27 (Mean ± SEM, 0.4771 ± 0.0895; p = 0.0001) and p16 (Mean ± SEM, 0.4676 ± 0.04305; p = 0.0001) at mRNA level. Concordant to semi-quantitative data, immunohistochemistry data also showed a significant upregulation of p27 (Mean ± SEM, 196.4 ± 10.84; p < 0.0001) and p16 (Mean ± SEM, 100.4 ± 23.71; p < 0.0001) at protein level. Conclusions The present study showed that the significant upregulation of p27 and p16 were associated with early events in gastric carcinogenesis. Our data suggests that clinical correlation of these differentially expressed genes may be useful as diagnostic biomarkers for early detection of gastric carcinoma and promising therapeutics target for GC patients.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_120 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702
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title_short	Expression of p27 and p16 and their clinical significance in gastric cancer
url	https://dx.doi.org/10.1007/s12094-020-02479-4
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author2	Kumar, P. Kumar, M. Singh, S. Narayan, G.
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up_date	2024-07-03T19:26:51.442Z
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Methods We recruited biopsies of a total of 84 subjects including 72 primary tumor biopsies from histopathologically proven gastric carcinoma, 8 adjacent controls and 12 independent controls. We used gastric cancer cell line, AGS, for validation of our data. Expression profiling at transcript level was done by semi-quantitative RT-PCR and at proteome level by immunohistochemistry and immunofluorescence. Receiver operator characteristics analysis was done for determining the diagnostic utility of p27 and p16 with respect to the sensitivity and specificity. Results We demonstrate that p27 and p16 are frequently over expressed in early stages of gastric carcinoma. Our semi-quantitative data show a significant upregulation of p27 (Mean ± SEM, 0.4771 ± 0.0895; p = 0.0001) and p16 (Mean ± SEM, 0.4676 ± 0.04305; p = 0.0001) at mRNA level. Concordant to semi-quantitative data, immunohistochemistry data also showed a significant upregulation of p27 (Mean ± SEM, 196.4 ± 10.84; p < 0.0001) and p16 (Mean ± SEM, 100.4 ± 23.71; p < 0.0001) at protein level. Conclusions The present study showed that the significant upregulation of p27 and p16 were associated with early events in gastric carcinogenesis. 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Expression of p27 and p16 and their clinical significance in gastric cancer

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