Clinical efficacy of an antibody-based detection system for human papilloma virus infection in oral squamous cell carcinoma
Objectives There is an increasing number of oral squamous cell carcinoma (OSCC) associated with HPV-16. However, p16 expression by immunohistochemistry as the current gold standard for a surrogate marker for virus infection reveals unsatisfying diagnostic accuracy. The aim of this study was to inves...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Blatt, Sebastian [verfasserIn] Pabst, Andreas [verfasserIn] Zimmer, Stefanie [verfasserIn] Walter, Christian [verfasserIn] Al-Nawas, Bilal [verfasserIn] Krüger, Maximilian [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Clinical Oral Investigations - Springer-Verlag, 2001, 25(2020), 5 vom: 23. Okt., Seite 2837-2843 |
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| Übergeordnetes Werk: |
volume:25 ; year:2020 ; number:5 ; day:23 ; month:10 ; pages:2837-2843 |
| Links: |
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| DOI / URN: |
10.1007/s00784-020-03601-0 |
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SPR043819419 |
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| 520 | |a Objectives There is an increasing number of oral squamous cell carcinoma (OSCC) associated with HPV-16. However, p16 expression by immunohistochemistry as the current gold standard for a surrogate marker for virus infection reveals unsatisfying diagnostic accuracy. The aim of this study was to investigate a new rapid test for L1 antibody detection (Prevocheck®) and to validate its diagnostic performance. Materials and methods In a prospective study, the HPV 16 association of all consecutive patients with an OSCC treated between 2015 and 2019 were analyzed by L1 seropositivity (via PrevoCheck®), p16 immunostaining, and partly multiplex PCR for subtype analysis. Results Overall (n = 107), p16 expression was positive in 17 cases (15.9%), and L1 antibody seropositivity in 7 cases (6.5%). In PCR analysis, two cases of HPV35 and 50 were found. Total HPV prevalence was 8.4% overall and 6.5% for HPV-16. An inferior diagnostic accuracy for HPV-16-associated OSCC in comparison to PrevoCheck® was revealed. Conclusion The rapid test for L1 antibodies showed an optimal sensitivity and specificity, positive and negative predictive value, and an overall diagnostic accuracy of 100%. However, HPV prevalence seems low in OSCC. Clinical relevance L1 rapid test may represent an additional diagnostic staging method to detect HPV-16 association rather than p16 immunohistochemistry. | ||
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| 650 | 4 | |a Head and neck cancer |7 (dpeaa)DE-He213 | |
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| 650 | 4 | |a p16 expression |7 (dpeaa)DE-He213 | |
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| 700 | 1 | |a Zimmer, Stefanie |e verfasserin |4 aut | |
| 700 | 1 | |a Walter, Christian |e verfasserin |4 aut | |
| 700 | 1 | |a Al-Nawas, Bilal |e verfasserin |4 aut | |
| 700 | 1 | |a Krüger, Maximilian |e verfasserin |4 aut | |
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10.1007/s00784-020-03601-0 doi (DE-627)SPR043819419 (DE-599)SPRs00784-020-03601-0-e (SPR)s00784-020-03601-0-e DE-627 ger DE-627 rakwb eng Blatt, Sebastian verfasserin aut Clinical efficacy of an antibody-based detection system for human papilloma virus infection in oral squamous cell carcinoma 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives There is an increasing number of oral squamous cell carcinoma (OSCC) associated with HPV-16. However, p16 expression by immunohistochemistry as the current gold standard for a surrogate marker for virus infection reveals unsatisfying diagnostic accuracy. The aim of this study was to investigate a new rapid test for L1 antibody detection (Prevocheck®) and to validate its diagnostic performance. Materials and methods In a prospective study, the HPV 16 association of all consecutive patients with an OSCC treated between 2015 and 2019 were analyzed by L1 seropositivity (via PrevoCheck®), p16 immunostaining, and partly multiplex PCR for subtype analysis. Results Overall (n = 107), p16 expression was positive in 17 cases (15.9%), and L1 antibody seropositivity in 7 cases (6.5%). In PCR analysis, two cases of HPV35 and 50 were found. Total HPV prevalence was 8.4% overall and 6.5% for HPV-16. An inferior diagnostic accuracy for HPV-16-associated OSCC in comparison to PrevoCheck® was revealed. Conclusion The rapid test for L1 antibodies showed an optimal sensitivity and specificity, positive and negative predictive value, and an overall diagnostic accuracy of 100%. However, HPV prevalence seems low in OSCC. Clinical relevance L1 rapid test may represent an additional diagnostic staging method to detect HPV-16 association rather than p16 immunohistochemistry. Oral cancer (dpeaa)DE-He213 Head and neck cancer (dpeaa)DE-He213 Human papilloma virus (dpeaa)DE-He213 HPV (dpeaa)DE-He213 p16 expression (dpeaa)DE-He213 Biomarker (dpeaa)DE-He213 Pabst, Andreas verfasserin aut Zimmer, Stefanie verfasserin aut Walter, Christian verfasserin aut Al-Nawas, Bilal verfasserin aut Krüger, Maximilian verfasserin aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 25(2020), 5 vom: 23. Okt., Seite 2837-2843 (DE-627)SPR007794231 nnns volume:25 year:2020 number:5 day:23 month:10 pages:2837-2843 https://dx.doi.org/10.1007/s00784-020-03601-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 25 2020 5 23 10 2837-2843 |
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10.1007/s00784-020-03601-0 doi (DE-627)SPR043819419 (DE-599)SPRs00784-020-03601-0-e (SPR)s00784-020-03601-0-e DE-627 ger DE-627 rakwb eng Blatt, Sebastian verfasserin aut Clinical efficacy of an antibody-based detection system for human papilloma virus infection in oral squamous cell carcinoma 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives There is an increasing number of oral squamous cell carcinoma (OSCC) associated with HPV-16. However, p16 expression by immunohistochemistry as the current gold standard for a surrogate marker for virus infection reveals unsatisfying diagnostic accuracy. The aim of this study was to investigate a new rapid test for L1 antibody detection (Prevocheck®) and to validate its diagnostic performance. Materials and methods In a prospective study, the HPV 16 association of all consecutive patients with an OSCC treated between 2015 and 2019 were analyzed by L1 seropositivity (via PrevoCheck®), p16 immunostaining, and partly multiplex PCR for subtype analysis. Results Overall (n = 107), p16 expression was positive in 17 cases (15.9%), and L1 antibody seropositivity in 7 cases (6.5%). In PCR analysis, two cases of HPV35 and 50 were found. Total HPV prevalence was 8.4% overall and 6.5% for HPV-16. An inferior diagnostic accuracy for HPV-16-associated OSCC in comparison to PrevoCheck® was revealed. Conclusion The rapid test for L1 antibodies showed an optimal sensitivity and specificity, positive and negative predictive value, and an overall diagnostic accuracy of 100%. However, HPV prevalence seems low in OSCC. Clinical relevance L1 rapid test may represent an additional diagnostic staging method to detect HPV-16 association rather than p16 immunohistochemistry. Oral cancer (dpeaa)DE-He213 Head and neck cancer (dpeaa)DE-He213 Human papilloma virus (dpeaa)DE-He213 HPV (dpeaa)DE-He213 p16 expression (dpeaa)DE-He213 Biomarker (dpeaa)DE-He213 Pabst, Andreas verfasserin aut Zimmer, Stefanie verfasserin aut Walter, Christian verfasserin aut Al-Nawas, Bilal verfasserin aut Krüger, Maximilian verfasserin aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 25(2020), 5 vom: 23. Okt., Seite 2837-2843 (DE-627)SPR007794231 nnns volume:25 year:2020 number:5 day:23 month:10 pages:2837-2843 https://dx.doi.org/10.1007/s00784-020-03601-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 25 2020 5 23 10 2837-2843 |
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10.1007/s00784-020-03601-0 doi (DE-627)SPR043819419 (DE-599)SPRs00784-020-03601-0-e (SPR)s00784-020-03601-0-e DE-627 ger DE-627 rakwb eng Blatt, Sebastian verfasserin aut Clinical efficacy of an antibody-based detection system for human papilloma virus infection in oral squamous cell carcinoma 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives There is an increasing number of oral squamous cell carcinoma (OSCC) associated with HPV-16. However, p16 expression by immunohistochemistry as the current gold standard for a surrogate marker for virus infection reveals unsatisfying diagnostic accuracy. The aim of this study was to investigate a new rapid test for L1 antibody detection (Prevocheck®) and to validate its diagnostic performance. Materials and methods In a prospective study, the HPV 16 association of all consecutive patients with an OSCC treated between 2015 and 2019 were analyzed by L1 seropositivity (via PrevoCheck®), p16 immunostaining, and partly multiplex PCR for subtype analysis. Results Overall (n = 107), p16 expression was positive in 17 cases (15.9%), and L1 antibody seropositivity in 7 cases (6.5%). In PCR analysis, two cases of HPV35 and 50 were found. Total HPV prevalence was 8.4% overall and 6.5% for HPV-16. An inferior diagnostic accuracy for HPV-16-associated OSCC in comparison to PrevoCheck® was revealed. Conclusion The rapid test for L1 antibodies showed an optimal sensitivity and specificity, positive and negative predictive value, and an overall diagnostic accuracy of 100%. However, HPV prevalence seems low in OSCC. Clinical relevance L1 rapid test may represent an additional diagnostic staging method to detect HPV-16 association rather than p16 immunohistochemistry. Oral cancer (dpeaa)DE-He213 Head and neck cancer (dpeaa)DE-He213 Human papilloma virus (dpeaa)DE-He213 HPV (dpeaa)DE-He213 p16 expression (dpeaa)DE-He213 Biomarker (dpeaa)DE-He213 Pabst, Andreas verfasserin aut Zimmer, Stefanie verfasserin aut Walter, Christian verfasserin aut Al-Nawas, Bilal verfasserin aut Krüger, Maximilian verfasserin aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 25(2020), 5 vom: 23. Okt., Seite 2837-2843 (DE-627)SPR007794231 nnns volume:25 year:2020 number:5 day:23 month:10 pages:2837-2843 https://dx.doi.org/10.1007/s00784-020-03601-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 25 2020 5 23 10 2837-2843 |
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10.1007/s00784-020-03601-0 doi (DE-627)SPR043819419 (DE-599)SPRs00784-020-03601-0-e (SPR)s00784-020-03601-0-e DE-627 ger DE-627 rakwb eng Blatt, Sebastian verfasserin aut Clinical efficacy of an antibody-based detection system for human papilloma virus infection in oral squamous cell carcinoma 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives There is an increasing number of oral squamous cell carcinoma (OSCC) associated with HPV-16. However, p16 expression by immunohistochemistry as the current gold standard for a surrogate marker for virus infection reveals unsatisfying diagnostic accuracy. The aim of this study was to investigate a new rapid test for L1 antibody detection (Prevocheck®) and to validate its diagnostic performance. Materials and methods In a prospective study, the HPV 16 association of all consecutive patients with an OSCC treated between 2015 and 2019 were analyzed by L1 seropositivity (via PrevoCheck®), p16 immunostaining, and partly multiplex PCR for subtype analysis. Results Overall (n = 107), p16 expression was positive in 17 cases (15.9%), and L1 antibody seropositivity in 7 cases (6.5%). In PCR analysis, two cases of HPV35 and 50 were found. Total HPV prevalence was 8.4% overall and 6.5% for HPV-16. An inferior diagnostic accuracy for HPV-16-associated OSCC in comparison to PrevoCheck® was revealed. Conclusion The rapid test for L1 antibodies showed an optimal sensitivity and specificity, positive and negative predictive value, and an overall diagnostic accuracy of 100%. However, HPV prevalence seems low in OSCC. Clinical relevance L1 rapid test may represent an additional diagnostic staging method to detect HPV-16 association rather than p16 immunohistochemistry. Oral cancer (dpeaa)DE-He213 Head and neck cancer (dpeaa)DE-He213 Human papilloma virus (dpeaa)DE-He213 HPV (dpeaa)DE-He213 p16 expression (dpeaa)DE-He213 Biomarker (dpeaa)DE-He213 Pabst, Andreas verfasserin aut Zimmer, Stefanie verfasserin aut Walter, Christian verfasserin aut Al-Nawas, Bilal verfasserin aut Krüger, Maximilian verfasserin aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 25(2020), 5 vom: 23. Okt., Seite 2837-2843 (DE-627)SPR007794231 nnns volume:25 year:2020 number:5 day:23 month:10 pages:2837-2843 https://dx.doi.org/10.1007/s00784-020-03601-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 25 2020 5 23 10 2837-2843 |
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10.1007/s00784-020-03601-0 doi (DE-627)SPR043819419 (DE-599)SPRs00784-020-03601-0-e (SPR)s00784-020-03601-0-e DE-627 ger DE-627 rakwb eng Blatt, Sebastian verfasserin aut Clinical efficacy of an antibody-based detection system for human papilloma virus infection in oral squamous cell carcinoma 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objectives There is an increasing number of oral squamous cell carcinoma (OSCC) associated with HPV-16. However, p16 expression by immunohistochemistry as the current gold standard for a surrogate marker for virus infection reveals unsatisfying diagnostic accuracy. The aim of this study was to investigate a new rapid test for L1 antibody detection (Prevocheck®) and to validate its diagnostic performance. Materials and methods In a prospective study, the HPV 16 association of all consecutive patients with an OSCC treated between 2015 and 2019 were analyzed by L1 seropositivity (via PrevoCheck®), p16 immunostaining, and partly multiplex PCR for subtype analysis. Results Overall (n = 107), p16 expression was positive in 17 cases (15.9%), and L1 antibody seropositivity in 7 cases (6.5%). In PCR analysis, two cases of HPV35 and 50 were found. Total HPV prevalence was 8.4% overall and 6.5% for HPV-16. An inferior diagnostic accuracy for HPV-16-associated OSCC in comparison to PrevoCheck® was revealed. Conclusion The rapid test for L1 antibodies showed an optimal sensitivity and specificity, positive and negative predictive value, and an overall diagnostic accuracy of 100%. However, HPV prevalence seems low in OSCC. Clinical relevance L1 rapid test may represent an additional diagnostic staging method to detect HPV-16 association rather than p16 immunohistochemistry. Oral cancer (dpeaa)DE-He213 Head and neck cancer (dpeaa)DE-He213 Human papilloma virus (dpeaa)DE-He213 HPV (dpeaa)DE-He213 p16 expression (dpeaa)DE-He213 Biomarker (dpeaa)DE-He213 Pabst, Andreas verfasserin aut Zimmer, Stefanie verfasserin aut Walter, Christian verfasserin aut Al-Nawas, Bilal verfasserin aut Krüger, Maximilian verfasserin aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 25(2020), 5 vom: 23. Okt., Seite 2837-2843 (DE-627)SPR007794231 nnns volume:25 year:2020 number:5 day:23 month:10 pages:2837-2843 https://dx.doi.org/10.1007/s00784-020-03601-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 25 2020 5 23 10 2837-2843 |
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Clinical efficacy of an antibody-based detection system for human papilloma virus infection in oral squamous cell carcinoma |
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Clinical efficacy of an antibody-based detection system for human papilloma virus infection in oral squamous cell carcinoma |
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Blatt, Sebastian |
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Clinical Oral Investigations |
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Clinical Oral Investigations |
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2020 |
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Blatt, Sebastian Pabst, Andreas Zimmer, Stefanie Walter, Christian Al-Nawas, Bilal Krüger, Maximilian |
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Elektronische Aufsätze |
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Blatt, Sebastian |
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10.1007/s00784-020-03601-0 |
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verfasserin |
| title_sort |
clinical efficacy of an antibody-based detection system for human papilloma virus infection in oral squamous cell carcinoma |
| title_auth |
Clinical efficacy of an antibody-based detection system for human papilloma virus infection in oral squamous cell carcinoma |
| abstract |
Objectives There is an increasing number of oral squamous cell carcinoma (OSCC) associated with HPV-16. However, p16 expression by immunohistochemistry as the current gold standard for a surrogate marker for virus infection reveals unsatisfying diagnostic accuracy. The aim of this study was to investigate a new rapid test for L1 antibody detection (Prevocheck®) and to validate its diagnostic performance. Materials and methods In a prospective study, the HPV 16 association of all consecutive patients with an OSCC treated between 2015 and 2019 were analyzed by L1 seropositivity (via PrevoCheck®), p16 immunostaining, and partly multiplex PCR for subtype analysis. Results Overall (n = 107), p16 expression was positive in 17 cases (15.9%), and L1 antibody seropositivity in 7 cases (6.5%). In PCR analysis, two cases of HPV35 and 50 were found. Total HPV prevalence was 8.4% overall and 6.5% for HPV-16. An inferior diagnostic accuracy for HPV-16-associated OSCC in comparison to PrevoCheck® was revealed. Conclusion The rapid test for L1 antibodies showed an optimal sensitivity and specificity, positive and negative predictive value, and an overall diagnostic accuracy of 100%. However, HPV prevalence seems low in OSCC. Clinical relevance L1 rapid test may represent an additional diagnostic staging method to detect HPV-16 association rather than p16 immunohistochemistry. |
| abstractGer |
Objectives There is an increasing number of oral squamous cell carcinoma (OSCC) associated with HPV-16. However, p16 expression by immunohistochemistry as the current gold standard for a surrogate marker for virus infection reveals unsatisfying diagnostic accuracy. The aim of this study was to investigate a new rapid test for L1 antibody detection (Prevocheck®) and to validate its diagnostic performance. Materials and methods In a prospective study, the HPV 16 association of all consecutive patients with an OSCC treated between 2015 and 2019 were analyzed by L1 seropositivity (via PrevoCheck®), p16 immunostaining, and partly multiplex PCR for subtype analysis. Results Overall (n = 107), p16 expression was positive in 17 cases (15.9%), and L1 antibody seropositivity in 7 cases (6.5%). In PCR analysis, two cases of HPV35 and 50 were found. Total HPV prevalence was 8.4% overall and 6.5% for HPV-16. An inferior diagnostic accuracy for HPV-16-associated OSCC in comparison to PrevoCheck® was revealed. Conclusion The rapid test for L1 antibodies showed an optimal sensitivity and specificity, positive and negative predictive value, and an overall diagnostic accuracy of 100%. However, HPV prevalence seems low in OSCC. Clinical relevance L1 rapid test may represent an additional diagnostic staging method to detect HPV-16 association rather than p16 immunohistochemistry. |
| abstract_unstemmed |
Objectives There is an increasing number of oral squamous cell carcinoma (OSCC) associated with HPV-16. However, p16 expression by immunohistochemistry as the current gold standard for a surrogate marker for virus infection reveals unsatisfying diagnostic accuracy. The aim of this study was to investigate a new rapid test for L1 antibody detection (Prevocheck®) and to validate its diagnostic performance. Materials and methods In a prospective study, the HPV 16 association of all consecutive patients with an OSCC treated between 2015 and 2019 were analyzed by L1 seropositivity (via PrevoCheck®), p16 immunostaining, and partly multiplex PCR for subtype analysis. Results Overall (n = 107), p16 expression was positive in 17 cases (15.9%), and L1 antibody seropositivity in 7 cases (6.5%). In PCR analysis, two cases of HPV35 and 50 were found. Total HPV prevalence was 8.4% overall and 6.5% for HPV-16. An inferior diagnostic accuracy for HPV-16-associated OSCC in comparison to PrevoCheck® was revealed. Conclusion The rapid test for L1 antibodies showed an optimal sensitivity and specificity, positive and negative predictive value, and an overall diagnostic accuracy of 100%. However, HPV prevalence seems low in OSCC. Clinical relevance L1 rapid test may represent an additional diagnostic staging method to detect HPV-16 association rather than p16 immunohistochemistry. |
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Clinical efficacy of an antibody-based detection system for human papilloma virus infection in oral squamous cell carcinoma |
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https://dx.doi.org/10.1007/s00784-020-03601-0 |
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Pabst, Andreas Zimmer, Stefanie Walter, Christian Al-Nawas, Bilal Krüger, Maximilian |
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2024-07-03T21:07:05.654Z |
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