A high-performance thin-layer chromatography method for the simultaneous determination of quercetin and gallic acid in Eclipta alba and Guiera senegalensis
Abstract Eclipta alba and Guiera senegalensis have traditionally been used in Ayurvedic and Unani medicine to cure several ailments. Due to the high demand, the quantitative standardization of plant-based products is challenging to maintain their quality. We aimed to develop and validate a high-perf...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Khalid, Mohammad [verfasserIn] Alqarni, Mohammed H. [verfasserIn] Foudah, Ahmed I. [verfasserIn] Alam, Prawez [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2021 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Journal of planar chromatography, modern TLC - Budapest : Akadémiai Kiadó, 2001, 34(2021), 1 vom: Feb., Seite 71-77 |
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| Übergeordnetes Werk: |
volume:34 ; year:2021 ; number:1 ; month:02 ; pages:71-77 |
| Links: |
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| DOI / URN: |
10.1007/s00764-021-00084-x |
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| Katalog-ID: |
SPR043833268 |
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| 100 | 1 | |a Khalid, Mohammad |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A high-performance thin-layer chromatography method for the simultaneous determination of quercetin and gallic acid in Eclipta alba and Guiera senegalensis |
| 264 | 1 | |c 2021 | |
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| 520 | |a Abstract Eclipta alba and Guiera senegalensis have traditionally been used in Ayurvedic and Unani medicine to cure several ailments. Due to the high demand, the quantitative standardization of plant-based products is challenging to maintain their quality. We aimed to develop and validate a high-performance thin-layer chromatography (HPTLC) method for the simultaneous detection and quantification of gallic acid and quercetin in E. alba and G. senegalensis, respectively. Aqueous extracts of both plants were prepared. Qualitative and quantitative analyses were performed for the simultaneous separation of gallic acid and quercetin in each extract as per the International Conference on Harmonization (ICH) guidelines with respect to linearity, specificity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) and robustness. HPTLC quantitative analyses for E. alba aqueous extracts revealed 3.83 µg/mg of gallic acid and 2.26 µg/mg quercetin, while 4.71 µg/mg of gallic acid and 1.06 µg/mg of quercetin for G. senegalensis. LOD was found to be 3.30 ng/band and 12.70 ng/band, while LOQ was found to be 10.20 and 38.60 ng/band for gallic acid and quercetin, respectively. The HPTLC method showed good linearity, recovery and robustness of the validated markers. This developed validated method is capable of quantifying and resolving gallic acid and quercetin and the quality control of E. alba and G. senegalensis. | ||
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| 650 | 4 | |a HPTLC |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Quercetin |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Alqarni, Mohammed H. |e verfasserin |4 aut | |
| 700 | 1 | |a Foudah, Ahmed I. |e verfasserin |4 aut | |
| 700 | 1 | |a Alam, Prawez |e verfasserin |4 aut | |
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10.1007/s00764-021-00084-x doi (DE-627)SPR043833268 (DE-599)SPRs00764-021-00084-x-e (SPR)s00764-021-00084-x-e DE-627 ger DE-627 rakwb eng 540 530 ASE Khalid, Mohammad verfasserin aut A high-performance thin-layer chromatography method for the simultaneous determination of quercetin and gallic acid in Eclipta alba and Guiera senegalensis 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Eclipta alba and Guiera senegalensis have traditionally been used in Ayurvedic and Unani medicine to cure several ailments. Due to the high demand, the quantitative standardization of plant-based products is challenging to maintain their quality. We aimed to develop and validate a high-performance thin-layer chromatography (HPTLC) method for the simultaneous detection and quantification of gallic acid and quercetin in E. alba and G. senegalensis, respectively. Aqueous extracts of both plants were prepared. Qualitative and quantitative analyses were performed for the simultaneous separation of gallic acid and quercetin in each extract as per the International Conference on Harmonization (ICH) guidelines with respect to linearity, specificity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) and robustness. HPTLC quantitative analyses for E. alba aqueous extracts revealed 3.83 µg/mg of gallic acid and 2.26 µg/mg quercetin, while 4.71 µg/mg of gallic acid and 1.06 µg/mg of quercetin for G. senegalensis. LOD was found to be 3.30 ng/band and 12.70 ng/band, while LOQ was found to be 10.20 and 38.60 ng/band for gallic acid and quercetin, respectively. The HPTLC method showed good linearity, recovery and robustness of the validated markers. This developed validated method is capable of quantifying and resolving gallic acid and quercetin and the quality control of E. alba and G. senegalensis. Gallic acid (dpeaa)DE-He213 HPTLC (dpeaa)DE-He213 Quercetin (dpeaa)DE-He213 Alqarni, Mohammed H. verfasserin aut Foudah, Ahmed I. verfasserin aut Alam, Prawez verfasserin aut Enthalten in Journal of planar chromatography, modern TLC Budapest : Akadémiai Kiadó, 2001 34(2021), 1 vom: Feb., Seite 71-77 (DE-627)529764563 (DE-600)2304198-5 1789-0993 nnns volume:34 year:2021 number:1 month:02 pages:71-77 https://dx.doi.org/10.1007/s00764-021-00084-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 34 2021 1 02 71-77 |
| spelling |
10.1007/s00764-021-00084-x doi (DE-627)SPR043833268 (DE-599)SPRs00764-021-00084-x-e (SPR)s00764-021-00084-x-e DE-627 ger DE-627 rakwb eng 540 530 ASE Khalid, Mohammad verfasserin aut A high-performance thin-layer chromatography method for the simultaneous determination of quercetin and gallic acid in Eclipta alba and Guiera senegalensis 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Eclipta alba and Guiera senegalensis have traditionally been used in Ayurvedic and Unani medicine to cure several ailments. Due to the high demand, the quantitative standardization of plant-based products is challenging to maintain their quality. We aimed to develop and validate a high-performance thin-layer chromatography (HPTLC) method for the simultaneous detection and quantification of gallic acid and quercetin in E. alba and G. senegalensis, respectively. Aqueous extracts of both plants were prepared. Qualitative and quantitative analyses were performed for the simultaneous separation of gallic acid and quercetin in each extract as per the International Conference on Harmonization (ICH) guidelines with respect to linearity, specificity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) and robustness. HPTLC quantitative analyses for E. alba aqueous extracts revealed 3.83 µg/mg of gallic acid and 2.26 µg/mg quercetin, while 4.71 µg/mg of gallic acid and 1.06 µg/mg of quercetin for G. senegalensis. LOD was found to be 3.30 ng/band and 12.70 ng/band, while LOQ was found to be 10.20 and 38.60 ng/band for gallic acid and quercetin, respectively. The HPTLC method showed good linearity, recovery and robustness of the validated markers. This developed validated method is capable of quantifying and resolving gallic acid and quercetin and the quality control of E. alba and G. senegalensis. Gallic acid (dpeaa)DE-He213 HPTLC (dpeaa)DE-He213 Quercetin (dpeaa)DE-He213 Alqarni, Mohammed H. verfasserin aut Foudah, Ahmed I. verfasserin aut Alam, Prawez verfasserin aut Enthalten in Journal of planar chromatography, modern TLC Budapest : Akadémiai Kiadó, 2001 34(2021), 1 vom: Feb., Seite 71-77 (DE-627)529764563 (DE-600)2304198-5 1789-0993 nnns volume:34 year:2021 number:1 month:02 pages:71-77 https://dx.doi.org/10.1007/s00764-021-00084-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 34 2021 1 02 71-77 |
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10.1007/s00764-021-00084-x doi (DE-627)SPR043833268 (DE-599)SPRs00764-021-00084-x-e (SPR)s00764-021-00084-x-e DE-627 ger DE-627 rakwb eng 540 530 ASE Khalid, Mohammad verfasserin aut A high-performance thin-layer chromatography method for the simultaneous determination of quercetin and gallic acid in Eclipta alba and Guiera senegalensis 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Eclipta alba and Guiera senegalensis have traditionally been used in Ayurvedic and Unani medicine to cure several ailments. Due to the high demand, the quantitative standardization of plant-based products is challenging to maintain their quality. We aimed to develop and validate a high-performance thin-layer chromatography (HPTLC) method for the simultaneous detection and quantification of gallic acid and quercetin in E. alba and G. senegalensis, respectively. Aqueous extracts of both plants were prepared. Qualitative and quantitative analyses were performed for the simultaneous separation of gallic acid and quercetin in each extract as per the International Conference on Harmonization (ICH) guidelines with respect to linearity, specificity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) and robustness. HPTLC quantitative analyses for E. alba aqueous extracts revealed 3.83 µg/mg of gallic acid and 2.26 µg/mg quercetin, while 4.71 µg/mg of gallic acid and 1.06 µg/mg of quercetin for G. senegalensis. LOD was found to be 3.30 ng/band and 12.70 ng/band, while LOQ was found to be 10.20 and 38.60 ng/band for gallic acid and quercetin, respectively. The HPTLC method showed good linearity, recovery and robustness of the validated markers. This developed validated method is capable of quantifying and resolving gallic acid and quercetin and the quality control of E. alba and G. senegalensis. Gallic acid (dpeaa)DE-He213 HPTLC (dpeaa)DE-He213 Quercetin (dpeaa)DE-He213 Alqarni, Mohammed H. verfasserin aut Foudah, Ahmed I. verfasserin aut Alam, Prawez verfasserin aut Enthalten in Journal of planar chromatography, modern TLC Budapest : Akadémiai Kiadó, 2001 34(2021), 1 vom: Feb., Seite 71-77 (DE-627)529764563 (DE-600)2304198-5 1789-0993 nnns volume:34 year:2021 number:1 month:02 pages:71-77 https://dx.doi.org/10.1007/s00764-021-00084-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 34 2021 1 02 71-77 |
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10.1007/s00764-021-00084-x doi (DE-627)SPR043833268 (DE-599)SPRs00764-021-00084-x-e (SPR)s00764-021-00084-x-e DE-627 ger DE-627 rakwb eng 540 530 ASE Khalid, Mohammad verfasserin aut A high-performance thin-layer chromatography method for the simultaneous determination of quercetin and gallic acid in Eclipta alba and Guiera senegalensis 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Eclipta alba and Guiera senegalensis have traditionally been used in Ayurvedic and Unani medicine to cure several ailments. Due to the high demand, the quantitative standardization of plant-based products is challenging to maintain their quality. We aimed to develop and validate a high-performance thin-layer chromatography (HPTLC) method for the simultaneous detection and quantification of gallic acid and quercetin in E. alba and G. senegalensis, respectively. Aqueous extracts of both plants were prepared. Qualitative and quantitative analyses were performed for the simultaneous separation of gallic acid and quercetin in each extract as per the International Conference on Harmonization (ICH) guidelines with respect to linearity, specificity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) and robustness. HPTLC quantitative analyses for E. alba aqueous extracts revealed 3.83 µg/mg of gallic acid and 2.26 µg/mg quercetin, while 4.71 µg/mg of gallic acid and 1.06 µg/mg of quercetin for G. senegalensis. LOD was found to be 3.30 ng/band and 12.70 ng/band, while LOQ was found to be 10.20 and 38.60 ng/band for gallic acid and quercetin, respectively. The HPTLC method showed good linearity, recovery and robustness of the validated markers. This developed validated method is capable of quantifying and resolving gallic acid and quercetin and the quality control of E. alba and G. senegalensis. Gallic acid (dpeaa)DE-He213 HPTLC (dpeaa)DE-He213 Quercetin (dpeaa)DE-He213 Alqarni, Mohammed H. verfasserin aut Foudah, Ahmed I. verfasserin aut Alam, Prawez verfasserin aut Enthalten in Journal of planar chromatography, modern TLC Budapest : Akadémiai Kiadó, 2001 34(2021), 1 vom: Feb., Seite 71-77 (DE-627)529764563 (DE-600)2304198-5 1789-0993 nnns volume:34 year:2021 number:1 month:02 pages:71-77 https://dx.doi.org/10.1007/s00764-021-00084-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 34 2021 1 02 71-77 |
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10.1007/s00764-021-00084-x doi (DE-627)SPR043833268 (DE-599)SPRs00764-021-00084-x-e (SPR)s00764-021-00084-x-e DE-627 ger DE-627 rakwb eng 540 530 ASE Khalid, Mohammad verfasserin aut A high-performance thin-layer chromatography method for the simultaneous determination of quercetin and gallic acid in Eclipta alba and Guiera senegalensis 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Eclipta alba and Guiera senegalensis have traditionally been used in Ayurvedic and Unani medicine to cure several ailments. Due to the high demand, the quantitative standardization of plant-based products is challenging to maintain their quality. We aimed to develop and validate a high-performance thin-layer chromatography (HPTLC) method for the simultaneous detection and quantification of gallic acid and quercetin in E. alba and G. senegalensis, respectively. Aqueous extracts of both plants were prepared. Qualitative and quantitative analyses were performed for the simultaneous separation of gallic acid and quercetin in each extract as per the International Conference on Harmonization (ICH) guidelines with respect to linearity, specificity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) and robustness. HPTLC quantitative analyses for E. alba aqueous extracts revealed 3.83 µg/mg of gallic acid and 2.26 µg/mg quercetin, while 4.71 µg/mg of gallic acid and 1.06 µg/mg of quercetin for G. senegalensis. LOD was found to be 3.30 ng/band and 12.70 ng/band, while LOQ was found to be 10.20 and 38.60 ng/band for gallic acid and quercetin, respectively. The HPTLC method showed good linearity, recovery and robustness of the validated markers. This developed validated method is capable of quantifying and resolving gallic acid and quercetin and the quality control of E. alba and G. senegalensis. Gallic acid (dpeaa)DE-He213 HPTLC (dpeaa)DE-He213 Quercetin (dpeaa)DE-He213 Alqarni, Mohammed H. verfasserin aut Foudah, Ahmed I. verfasserin aut Alam, Prawez verfasserin aut Enthalten in Journal of planar chromatography, modern TLC Budapest : Akadémiai Kiadó, 2001 34(2021), 1 vom: Feb., Seite 71-77 (DE-627)529764563 (DE-600)2304198-5 1789-0993 nnns volume:34 year:2021 number:1 month:02 pages:71-77 https://dx.doi.org/10.1007/s00764-021-00084-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 34 2021 1 02 71-77 |
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Enthalten in Journal of planar chromatography, modern TLC 34(2021), 1 vom: Feb., Seite 71-77 volume:34 year:2021 number:1 month:02 pages:71-77 |
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Gallic acid HPTLC Quercetin |
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Khalid, Mohammad @@aut@@ Alqarni, Mohammed H. @@aut@@ Foudah, Ahmed I. @@aut@@ Alam, Prawez @@aut@@ |
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Due to the high demand, the quantitative standardization of plant-based products is challenging to maintain their quality. We aimed to develop and validate a high-performance thin-layer chromatography (HPTLC) method for the simultaneous detection and quantification of gallic acid and quercetin in E. alba and G. senegalensis, respectively. Aqueous extracts of both plants were prepared. Qualitative and quantitative analyses were performed for the simultaneous separation of gallic acid and quercetin in each extract as per the International Conference on Harmonization (ICH) guidelines with respect to linearity, specificity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) and robustness. HPTLC quantitative analyses for E. alba aqueous extracts revealed 3.83 µg/mg of gallic acid and 2.26 µg/mg quercetin, while 4.71 µg/mg of gallic acid and 1.06 µg/mg of quercetin for G. senegalensis. LOD was found to be 3.30 ng/band and 12.70 ng/band, while LOQ was found to be 10.20 and 38.60 ng/band for gallic acid and quercetin, respectively. The HPTLC method showed good linearity, recovery and robustness of the validated markers. This developed validated method is capable of quantifying and resolving gallic acid and quercetin and the quality control of E. alba and G. senegalensis.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Gallic acid</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">HPTLC</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Quercetin</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Alqarni, Mohammed H.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Foudah, Ahmed I.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Alam, 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Khalid, Mohammad |
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Khalid, Mohammad ddc 540 misc Gallic acid misc HPTLC misc Quercetin A high-performance thin-layer chromatography method for the simultaneous determination of quercetin and gallic acid in Eclipta alba and Guiera senegalensis |
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540 530 ASE A high-performance thin-layer chromatography method for the simultaneous determination of quercetin and gallic acid in Eclipta alba and Guiera senegalensis Gallic acid (dpeaa)DE-He213 HPTLC (dpeaa)DE-He213 Quercetin (dpeaa)DE-He213 |
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A high-performance thin-layer chromatography method for the simultaneous determination of quercetin and gallic acid in Eclipta alba and Guiera senegalensis |
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A high-performance thin-layer chromatography method for the simultaneous determination of quercetin and gallic acid in Eclipta alba and Guiera senegalensis |
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Khalid, Mohammad |
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Journal of planar chromatography, modern TLC |
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Khalid, Mohammad Alqarni, Mohammed H. Foudah, Ahmed I. Alam, Prawez |
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high-performance thin-layer chromatography method for the simultaneous determination of quercetin and gallic acid in eclipta alba and guiera senegalensis |
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A high-performance thin-layer chromatography method for the simultaneous determination of quercetin and gallic acid in Eclipta alba and Guiera senegalensis |
| abstract |
Abstract Eclipta alba and Guiera senegalensis have traditionally been used in Ayurvedic and Unani medicine to cure several ailments. Due to the high demand, the quantitative standardization of plant-based products is challenging to maintain their quality. We aimed to develop and validate a high-performance thin-layer chromatography (HPTLC) method for the simultaneous detection and quantification of gallic acid and quercetin in E. alba and G. senegalensis, respectively. Aqueous extracts of both plants were prepared. Qualitative and quantitative analyses were performed for the simultaneous separation of gallic acid and quercetin in each extract as per the International Conference on Harmonization (ICH) guidelines with respect to linearity, specificity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) and robustness. HPTLC quantitative analyses for E. alba aqueous extracts revealed 3.83 µg/mg of gallic acid and 2.26 µg/mg quercetin, while 4.71 µg/mg of gallic acid and 1.06 µg/mg of quercetin for G. senegalensis. LOD was found to be 3.30 ng/band and 12.70 ng/band, while LOQ was found to be 10.20 and 38.60 ng/band for gallic acid and quercetin, respectively. The HPTLC method showed good linearity, recovery and robustness of the validated markers. This developed validated method is capable of quantifying and resolving gallic acid and quercetin and the quality control of E. alba and G. senegalensis. |
| abstractGer |
Abstract Eclipta alba and Guiera senegalensis have traditionally been used in Ayurvedic and Unani medicine to cure several ailments. Due to the high demand, the quantitative standardization of plant-based products is challenging to maintain their quality. We aimed to develop and validate a high-performance thin-layer chromatography (HPTLC) method for the simultaneous detection and quantification of gallic acid and quercetin in E. alba and G. senegalensis, respectively. Aqueous extracts of both plants were prepared. Qualitative and quantitative analyses were performed for the simultaneous separation of gallic acid and quercetin in each extract as per the International Conference on Harmonization (ICH) guidelines with respect to linearity, specificity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) and robustness. HPTLC quantitative analyses for E. alba aqueous extracts revealed 3.83 µg/mg of gallic acid and 2.26 µg/mg quercetin, while 4.71 µg/mg of gallic acid and 1.06 µg/mg of quercetin for G. senegalensis. LOD was found to be 3.30 ng/band and 12.70 ng/band, while LOQ was found to be 10.20 and 38.60 ng/band for gallic acid and quercetin, respectively. The HPTLC method showed good linearity, recovery and robustness of the validated markers. This developed validated method is capable of quantifying and resolving gallic acid and quercetin and the quality control of E. alba and G. senegalensis. |
| abstract_unstemmed |
Abstract Eclipta alba and Guiera senegalensis have traditionally been used in Ayurvedic and Unani medicine to cure several ailments. Due to the high demand, the quantitative standardization of plant-based products is challenging to maintain their quality. We aimed to develop and validate a high-performance thin-layer chromatography (HPTLC) method for the simultaneous detection and quantification of gallic acid and quercetin in E. alba and G. senegalensis, respectively. Aqueous extracts of both plants were prepared. Qualitative and quantitative analyses were performed for the simultaneous separation of gallic acid and quercetin in each extract as per the International Conference on Harmonization (ICH) guidelines with respect to linearity, specificity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) and robustness. HPTLC quantitative analyses for E. alba aqueous extracts revealed 3.83 µg/mg of gallic acid and 2.26 µg/mg quercetin, while 4.71 µg/mg of gallic acid and 1.06 µg/mg of quercetin for G. senegalensis. LOD was found to be 3.30 ng/band and 12.70 ng/band, while LOQ was found to be 10.20 and 38.60 ng/band for gallic acid and quercetin, respectively. The HPTLC method showed good linearity, recovery and robustness of the validated markers. This developed validated method is capable of quantifying and resolving gallic acid and quercetin and the quality control of E. alba and G. senegalensis. |
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| title_short |
A high-performance thin-layer chromatography method for the simultaneous determination of quercetin and gallic acid in Eclipta alba and Guiera senegalensis |
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https://dx.doi.org/10.1007/s00764-021-00084-x |
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| author2 |
Alqarni, Mohammed H. Foudah, Ahmed I. Alam, Prawez |
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Alqarni, Mohammed H. Foudah, Ahmed I. Alam, Prawez |
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10.1007/s00764-021-00084-x |
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2024-07-03T21:13:17.194Z |
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| score |
7.400094 |