Highly efficient production of transfructosylating enzymes using low-cost sugarcane molasses by A. pullulans FRR 5284

Abstract Fructooligosaccharides (FOS) are a type of important prebiotics and produced by transfructosylating enzymes. In this study, sugarcane molasses was used as the substrate for production of transfructosylating enzymes by Aureobasidium pullulans FRR 5284. $ NaNO_{3} $ was a superior nitrogen so...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Khatun, Most Sheauly [verfasserIn] Hassanpour, Morteza [verfasserIn] Harrison, Mark D. [verfasserIn] Speight, Robert E. [verfasserIn] O’Hara, Ian M. [verfasserIn] Zhang, Zhanying [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	Molasses Transfructosylation Fructooligosaccharides Nitrogen Scale-up

Anmerkung:	© The Author(s) 2021

Übergeordnetes Werk:	Enthalten in: Bioresources and Bioprocessing - Berlin : SpringerOpen, 2014, 8(2021), 1 vom: 11. Juni
Übergeordnetes Werk:	volume:8 ; year:2021 ; number:1 ; day:11 ; month:06

Links:	Volltext

DOI / URN:	10.1186/s40643-021-00399-x

Katalog-ID:	SPR044287178

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520			\|a Abstract Fructooligosaccharides (FOS) are a type of important prebiotics and produced by transfructosylating enzymes. In this study, sugarcane molasses was used as the substrate for production of transfructosylating enzymes by Aureobasidium pullulans FRR 5284. $ NaNO_{3} $ was a superior nitrogen source to yeast extract for production of transfructosylating enzymes by A. pullulans FRR 5284 and decreasing the ratio of $ NaNO_{3} $ to yeast extract nitrogen from 1:0 to 1:1 resulted in the reduction of the total transfructosylating activity from 109.8 U/mL to 82.5 U/mL. The addition of only 4.4 g/L $ NaNO_{3} $ into molasses-based medium containing 100 g/L mono- and di-saccharides resulted in total transfructosylating activity of 123.8 U/mL. Scale-up of the A. pullulans FRR 5284 transfructosylating enzyme production process from shake flasks to 1 L bioreactors improved the enzyme activity and productivity to 171.7 U/mL and 3.58 U/mL/h, 39% and 108% higher than those achieved from shake flasks, respectively. Sucrose (500 g/L) was used as a substrate for extracellular, intracellular, and total A. pullulans FRR 5284 transfructosylating enzymes, with a maximum yield of 61%. Intracellular, extracellular, and total A. pullulans FRR 5284 transfructosylating enzymes from different production systems resulted in different FOS profiles, indicating that FOS profiles can be controlled by adjusting intracellular and extracellular enzyme ratios and, hence prebiotic activity.
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Indexfelder

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allfields	10.1186/s40643-021-00399-x doi (DE-627)SPR044287178 (SPR)s40643-021-00399-x-e DE-627 ger DE-627 rakwb eng 570 ASE Khatun, Most Sheauly verfasserin aut Highly efficient production of transfructosylating enzymes using low-cost sugarcane molasses by A. pullulans FRR 5284 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2021 Abstract Fructooligosaccharides (FOS) are a type of important prebiotics and produced by transfructosylating enzymes. In this study, sugarcane molasses was used as the substrate for production of transfructosylating enzymes by Aureobasidium pullulans FRR 5284. $ NaNO_{3} $ was a superior nitrogen source to yeast extract for production of transfructosylating enzymes by A. pullulans FRR 5284 and decreasing the ratio of $ NaNO_{3} $ to yeast extract nitrogen from 1:0 to 1:1 resulted in the reduction of the total transfructosylating activity from 109.8 U/mL to 82.5 U/mL. The addition of only 4.4 g/L $ NaNO_{3} $ into molasses-based medium containing 100 g/L mono- and di-saccharides resulted in total transfructosylating activity of 123.8 U/mL. Scale-up of the A. pullulans FRR 5284 transfructosylating enzyme production process from shake flasks to 1 L bioreactors improved the enzyme activity and productivity to 171.7 U/mL and 3.58 U/mL/h, 39% and 108% higher than those achieved from shake flasks, respectively. Sucrose (500 g/L) was used as a substrate for extracellular, intracellular, and total A. pullulans FRR 5284 transfructosylating enzymes, with a maximum yield of 61%. Intracellular, extracellular, and total A. pullulans FRR 5284 transfructosylating enzymes from different production systems resulted in different FOS profiles, indicating that FOS profiles can be controlled by adjusting intracellular and extracellular enzyme ratios and, hence prebiotic activity. Molasses (dpeaa)DE-He213 Transfructosylation (dpeaa)DE-He213 Fructooligosaccharides (dpeaa)DE-He213 Nitrogen (dpeaa)DE-He213 Scale-up (dpeaa)DE-He213 Hassanpour, Morteza verfasserin aut Harrison, Mark D. verfasserin aut Speight, Robert E. verfasserin aut O’Hara, Ian M. verfasserin aut Zhang, Zhanying verfasserin aut Enthalten in Bioresources and Bioprocessing Berlin : SpringerOpen, 2014 8(2021), 1 vom: 11. Juni (DE-627)79738166X (DE-600)2785482-6 2197-4365 nnns volume:8 year:2021 number:1 day:11 month:06 https://dx.doi.org/10.1186/s40643-021-00399-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2021 1 11 06
spelling	10.1186/s40643-021-00399-x doi (DE-627)SPR044287178 (SPR)s40643-021-00399-x-e DE-627 ger DE-627 rakwb eng 570 ASE Khatun, Most Sheauly verfasserin aut Highly efficient production of transfructosylating enzymes using low-cost sugarcane molasses by A. pullulans FRR 5284 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2021 Abstract Fructooligosaccharides (FOS) are a type of important prebiotics and produced by transfructosylating enzymes. In this study, sugarcane molasses was used as the substrate for production of transfructosylating enzymes by Aureobasidium pullulans FRR 5284. $ NaNO_{3} $ was a superior nitrogen source to yeast extract for production of transfructosylating enzymes by A. pullulans FRR 5284 and decreasing the ratio of $ NaNO_{3} $ to yeast extract nitrogen from 1:0 to 1:1 resulted in the reduction of the total transfructosylating activity from 109.8 U/mL to 82.5 U/mL. The addition of only 4.4 g/L $ NaNO_{3} $ into molasses-based medium containing 100 g/L mono- and di-saccharides resulted in total transfructosylating activity of 123.8 U/mL. Scale-up of the A. pullulans FRR 5284 transfructosylating enzyme production process from shake flasks to 1 L bioreactors improved the enzyme activity and productivity to 171.7 U/mL and 3.58 U/mL/h, 39% and 108% higher than those achieved from shake flasks, respectively. Sucrose (500 g/L) was used as a substrate for extracellular, intracellular, and total A. pullulans FRR 5284 transfructosylating enzymes, with a maximum yield of 61%. Intracellular, extracellular, and total A. pullulans FRR 5284 transfructosylating enzymes from different production systems resulted in different FOS profiles, indicating that FOS profiles can be controlled by adjusting intracellular and extracellular enzyme ratios and, hence prebiotic activity. Molasses (dpeaa)DE-He213 Transfructosylation (dpeaa)DE-He213 Fructooligosaccharides (dpeaa)DE-He213 Nitrogen (dpeaa)DE-He213 Scale-up (dpeaa)DE-He213 Hassanpour, Morteza verfasserin aut Harrison, Mark D. verfasserin aut Speight, Robert E. verfasserin aut O’Hara, Ian M. verfasserin aut Zhang, Zhanying verfasserin aut Enthalten in Bioresources and Bioprocessing Berlin : SpringerOpen, 2014 8(2021), 1 vom: 11. Juni (DE-627)79738166X (DE-600)2785482-6 2197-4365 nnns volume:8 year:2021 number:1 day:11 month:06 https://dx.doi.org/10.1186/s40643-021-00399-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2021 1 11 06
allfields_unstemmed	10.1186/s40643-021-00399-x doi (DE-627)SPR044287178 (SPR)s40643-021-00399-x-e DE-627 ger DE-627 rakwb eng 570 ASE Khatun, Most Sheauly verfasserin aut Highly efficient production of transfructosylating enzymes using low-cost sugarcane molasses by A. pullulans FRR 5284 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2021 Abstract Fructooligosaccharides (FOS) are a type of important prebiotics and produced by transfructosylating enzymes. In this study, sugarcane molasses was used as the substrate for production of transfructosylating enzymes by Aureobasidium pullulans FRR 5284. $ NaNO_{3} $ was a superior nitrogen source to yeast extract for production of transfructosylating enzymes by A. pullulans FRR 5284 and decreasing the ratio of $ NaNO_{3} $ to yeast extract nitrogen from 1:0 to 1:1 resulted in the reduction of the total transfructosylating activity from 109.8 U/mL to 82.5 U/mL. The addition of only 4.4 g/L $ NaNO_{3} $ into molasses-based medium containing 100 g/L mono- and di-saccharides resulted in total transfructosylating activity of 123.8 U/mL. Scale-up of the A. pullulans FRR 5284 transfructosylating enzyme production process from shake flasks to 1 L bioreactors improved the enzyme activity and productivity to 171.7 U/mL and 3.58 U/mL/h, 39% and 108% higher than those achieved from shake flasks, respectively. Sucrose (500 g/L) was used as a substrate for extracellular, intracellular, and total A. pullulans FRR 5284 transfructosylating enzymes, with a maximum yield of 61%. Intracellular, extracellular, and total A. pullulans FRR 5284 transfructosylating enzymes from different production systems resulted in different FOS profiles, indicating that FOS profiles can be controlled by adjusting intracellular and extracellular enzyme ratios and, hence prebiotic activity. Molasses (dpeaa)DE-He213 Transfructosylation (dpeaa)DE-He213 Fructooligosaccharides (dpeaa)DE-He213 Nitrogen (dpeaa)DE-He213 Scale-up (dpeaa)DE-He213 Hassanpour, Morteza verfasserin aut Harrison, Mark D. verfasserin aut Speight, Robert E. verfasserin aut O’Hara, Ian M. verfasserin aut Zhang, Zhanying verfasserin aut Enthalten in Bioresources and Bioprocessing Berlin : SpringerOpen, 2014 8(2021), 1 vom: 11. Juni (DE-627)79738166X (DE-600)2785482-6 2197-4365 nnns volume:8 year:2021 number:1 day:11 month:06 https://dx.doi.org/10.1186/s40643-021-00399-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2021 1 11 06
allfieldsGer	10.1186/s40643-021-00399-x doi (DE-627)SPR044287178 (SPR)s40643-021-00399-x-e DE-627 ger DE-627 rakwb eng 570 ASE Khatun, Most Sheauly verfasserin aut Highly efficient production of transfructosylating enzymes using low-cost sugarcane molasses by A. pullulans FRR 5284 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2021 Abstract Fructooligosaccharides (FOS) are a type of important prebiotics and produced by transfructosylating enzymes. In this study, sugarcane molasses was used as the substrate for production of transfructosylating enzymes by Aureobasidium pullulans FRR 5284. $ NaNO_{3} $ was a superior nitrogen source to yeast extract for production of transfructosylating enzymes by A. pullulans FRR 5284 and decreasing the ratio of $ NaNO_{3} $ to yeast extract nitrogen from 1:0 to 1:1 resulted in the reduction of the total transfructosylating activity from 109.8 U/mL to 82.5 U/mL. The addition of only 4.4 g/L $ NaNO_{3} $ into molasses-based medium containing 100 g/L mono- and di-saccharides resulted in total transfructosylating activity of 123.8 U/mL. Scale-up of the A. pullulans FRR 5284 transfructosylating enzyme production process from shake flasks to 1 L bioreactors improved the enzyme activity and productivity to 171.7 U/mL and 3.58 U/mL/h, 39% and 108% higher than those achieved from shake flasks, respectively. Sucrose (500 g/L) was used as a substrate for extracellular, intracellular, and total A. pullulans FRR 5284 transfructosylating enzymes, with a maximum yield of 61%. Intracellular, extracellular, and total A. pullulans FRR 5284 transfructosylating enzymes from different production systems resulted in different FOS profiles, indicating that FOS profiles can be controlled by adjusting intracellular and extracellular enzyme ratios and, hence prebiotic activity. Molasses (dpeaa)DE-He213 Transfructosylation (dpeaa)DE-He213 Fructooligosaccharides (dpeaa)DE-He213 Nitrogen (dpeaa)DE-He213 Scale-up (dpeaa)DE-He213 Hassanpour, Morteza verfasserin aut Harrison, Mark D. verfasserin aut Speight, Robert E. verfasserin aut O’Hara, Ian M. verfasserin aut Zhang, Zhanying verfasserin aut Enthalten in Bioresources and Bioprocessing Berlin : SpringerOpen, 2014 8(2021), 1 vom: 11. Juni (DE-627)79738166X (DE-600)2785482-6 2197-4365 nnns volume:8 year:2021 number:1 day:11 month:06 https://dx.doi.org/10.1186/s40643-021-00399-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2021 1 11 06
allfieldsSound	10.1186/s40643-021-00399-x doi (DE-627)SPR044287178 (SPR)s40643-021-00399-x-e DE-627 ger DE-627 rakwb eng 570 ASE Khatun, Most Sheauly verfasserin aut Highly efficient production of transfructosylating enzymes using low-cost sugarcane molasses by A. pullulans FRR 5284 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2021 Abstract Fructooligosaccharides (FOS) are a type of important prebiotics and produced by transfructosylating enzymes. In this study, sugarcane molasses was used as the substrate for production of transfructosylating enzymes by Aureobasidium pullulans FRR 5284. $ NaNO_{3} $ was a superior nitrogen source to yeast extract for production of transfructosylating enzymes by A. pullulans FRR 5284 and decreasing the ratio of $ NaNO_{3} $ to yeast extract nitrogen from 1:0 to 1:1 resulted in the reduction of the total transfructosylating activity from 109.8 U/mL to 82.5 U/mL. The addition of only 4.4 g/L $ NaNO_{3} $ into molasses-based medium containing 100 g/L mono- and di-saccharides resulted in total transfructosylating activity of 123.8 U/mL. Scale-up of the A. pullulans FRR 5284 transfructosylating enzyme production process from shake flasks to 1 L bioreactors improved the enzyme activity and productivity to 171.7 U/mL and 3.58 U/mL/h, 39% and 108% higher than those achieved from shake flasks, respectively. Sucrose (500 g/L) was used as a substrate for extracellular, intracellular, and total A. pullulans FRR 5284 transfructosylating enzymes, with a maximum yield of 61%. Intracellular, extracellular, and total A. pullulans FRR 5284 transfructosylating enzymes from different production systems resulted in different FOS profiles, indicating that FOS profiles can be controlled by adjusting intracellular and extracellular enzyme ratios and, hence prebiotic activity. Molasses (dpeaa)DE-He213 Transfructosylation (dpeaa)DE-He213 Fructooligosaccharides (dpeaa)DE-He213 Nitrogen (dpeaa)DE-He213 Scale-up (dpeaa)DE-He213 Hassanpour, Morteza verfasserin aut Harrison, Mark D. verfasserin aut Speight, Robert E. verfasserin aut O’Hara, Ian M. verfasserin aut Zhang, Zhanying verfasserin aut Enthalten in Bioresources and Bioprocessing Berlin : SpringerOpen, 2014 8(2021), 1 vom: 11. Juni (DE-627)79738166X (DE-600)2785482-6 2197-4365 nnns volume:8 year:2021 number:1 day:11 month:06 https://dx.doi.org/10.1186/s40643-021-00399-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2021 1 11 06
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source	Enthalten in Bioresources and Bioprocessing 8(2021), 1 vom: 11. Juni volume:8 year:2021 number:1 day:11 month:06
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topic_facet	Molasses Transfructosylation Fructooligosaccharides Nitrogen Scale-up
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author	Khatun, Most Sheauly
spellingShingle	Khatun, Most Sheauly ddc 570 misc Molasses misc Transfructosylation misc Fructooligosaccharides misc Nitrogen misc Scale-up Highly efficient production of transfructosylating enzymes using low-cost sugarcane molasses by A. pullulans FRR 5284
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topic_title	570 ASE Highly efficient production of transfructosylating enzymes using low-cost sugarcane molasses by A. pullulans FRR 5284 Molasses (dpeaa)DE-He213 Transfructosylation (dpeaa)DE-He213 Fructooligosaccharides (dpeaa)DE-He213 Nitrogen (dpeaa)DE-He213 Scale-up (dpeaa)DE-He213
topic	ddc 570 misc Molasses misc Transfructosylation misc Fructooligosaccharides misc Nitrogen misc Scale-up
topic_unstemmed	ddc 570 misc Molasses misc Transfructosylation misc Fructooligosaccharides misc Nitrogen misc Scale-up
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title	Highly efficient production of transfructosylating enzymes using low-cost sugarcane molasses by A. pullulans FRR 5284
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title_full	Highly efficient production of transfructosylating enzymes using low-cost sugarcane molasses by A. pullulans FRR 5284
author_sort	Khatun, Most Sheauly
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author_browse	Khatun, Most Sheauly Hassanpour, Morteza Harrison, Mark D. Speight, Robert E. O’Hara, Ian M. Zhang, Zhanying
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title_sort	highly efficient production of transfructosylating enzymes using low-cost sugarcane molasses by a. pullulans frr 5284
title_auth	Highly efficient production of transfructosylating enzymes using low-cost sugarcane molasses by A. pullulans FRR 5284
abstract	Abstract Fructooligosaccharides (FOS) are a type of important prebiotics and produced by transfructosylating enzymes. In this study, sugarcane molasses was used as the substrate for production of transfructosylating enzymes by Aureobasidium pullulans FRR 5284. $ NaNO_{3} $ was a superior nitrogen source to yeast extract for production of transfructosylating enzymes by A. pullulans FRR 5284 and decreasing the ratio of $ NaNO_{3} $ to yeast extract nitrogen from 1:0 to 1:1 resulted in the reduction of the total transfructosylating activity from 109.8 U/mL to 82.5 U/mL. The addition of only 4.4 g/L $ NaNO_{3} $ into molasses-based medium containing 100 g/L mono- and di-saccharides resulted in total transfructosylating activity of 123.8 U/mL. Scale-up of the A. pullulans FRR 5284 transfructosylating enzyme production process from shake flasks to 1 L bioreactors improved the enzyme activity and productivity to 171.7 U/mL and 3.58 U/mL/h, 39% and 108% higher than those achieved from shake flasks, respectively. Sucrose (500 g/L) was used as a substrate for extracellular, intracellular, and total A. pullulans FRR 5284 transfructosylating enzymes, with a maximum yield of 61%. Intracellular, extracellular, and total A. pullulans FRR 5284 transfructosylating enzymes from different production systems resulted in different FOS profiles, indicating that FOS profiles can be controlled by adjusting intracellular and extracellular enzyme ratios and, hence prebiotic activity. © The Author(s) 2021
abstractGer	Abstract Fructooligosaccharides (FOS) are a type of important prebiotics and produced by transfructosylating enzymes. In this study, sugarcane molasses was used as the substrate for production of transfructosylating enzymes by Aureobasidium pullulans FRR 5284. $ NaNO_{3} $ was a superior nitrogen source to yeast extract for production of transfructosylating enzymes by A. pullulans FRR 5284 and decreasing the ratio of $ NaNO_{3} $ to yeast extract nitrogen from 1:0 to 1:1 resulted in the reduction of the total transfructosylating activity from 109.8 U/mL to 82.5 U/mL. The addition of only 4.4 g/L $ NaNO_{3} $ into molasses-based medium containing 100 g/L mono- and di-saccharides resulted in total transfructosylating activity of 123.8 U/mL. Scale-up of the A. pullulans FRR 5284 transfructosylating enzyme production process from shake flasks to 1 L bioreactors improved the enzyme activity and productivity to 171.7 U/mL and 3.58 U/mL/h, 39% and 108% higher than those achieved from shake flasks, respectively. Sucrose (500 g/L) was used as a substrate for extracellular, intracellular, and total A. pullulans FRR 5284 transfructosylating enzymes, with a maximum yield of 61%. Intracellular, extracellular, and total A. pullulans FRR 5284 transfructosylating enzymes from different production systems resulted in different FOS profiles, indicating that FOS profiles can be controlled by adjusting intracellular and extracellular enzyme ratios and, hence prebiotic activity. © The Author(s) 2021
abstract_unstemmed	Abstract Fructooligosaccharides (FOS) are a type of important prebiotics and produced by transfructosylating enzymes. In this study, sugarcane molasses was used as the substrate for production of transfructosylating enzymes by Aureobasidium pullulans FRR 5284. $ NaNO_{3} $ was a superior nitrogen source to yeast extract for production of transfructosylating enzymes by A. pullulans FRR 5284 and decreasing the ratio of $ NaNO_{3} $ to yeast extract nitrogen from 1:0 to 1:1 resulted in the reduction of the total transfructosylating activity from 109.8 U/mL to 82.5 U/mL. The addition of only 4.4 g/L $ NaNO_{3} $ into molasses-based medium containing 100 g/L mono- and di-saccharides resulted in total transfructosylating activity of 123.8 U/mL. Scale-up of the A. pullulans FRR 5284 transfructosylating enzyme production process from shake flasks to 1 L bioreactors improved the enzyme activity and productivity to 171.7 U/mL and 3.58 U/mL/h, 39% and 108% higher than those achieved from shake flasks, respectively. Sucrose (500 g/L) was used as a substrate for extracellular, intracellular, and total A. pullulans FRR 5284 transfructosylating enzymes, with a maximum yield of 61%. Intracellular, extracellular, and total A. pullulans FRR 5284 transfructosylating enzymes from different production systems resulted in different FOS profiles, indicating that FOS profiles can be controlled by adjusting intracellular and extracellular enzyme ratios and, hence prebiotic activity. © The Author(s) 2021
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
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title_short	Highly efficient production of transfructosylating enzymes using low-cost sugarcane molasses by A. pullulans FRR 5284
url	https://dx.doi.org/10.1186/s40643-021-00399-x
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author2	Hassanpour, Morteza Harrison, Mark D. Speight, Robert E. O’Hara, Ian M. Zhang, Zhanying
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up_date	2024-07-03T23:56:04.337Z
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In this study, sugarcane molasses was used as the substrate for production of transfructosylating enzymes by Aureobasidium pullulans FRR 5284. $ NaNO_{3} $ was a superior nitrogen source to yeast extract for production of transfructosylating enzymes by A. pullulans FRR 5284 and decreasing the ratio of $ NaNO_{3} $ to yeast extract nitrogen from 1:0 to 1:1 resulted in the reduction of the total transfructosylating activity from 109.8 U/mL to 82.5 U/mL. The addition of only 4.4 g/L $ NaNO_{3} $ into molasses-based medium containing 100 g/L mono- and di-saccharides resulted in total transfructosylating activity of 123.8 U/mL. Scale-up of the A. pullulans FRR 5284 transfructosylating enzyme production process from shake flasks to 1 L bioreactors improved the enzyme activity and productivity to 171.7 U/mL and 3.58 U/mL/h, 39% and 108% higher than those achieved from shake flasks, respectively. Sucrose (500 g/L) was used as a substrate for extracellular, intracellular, and total A. pullulans FRR 5284 transfructosylating enzymes, with a maximum yield of 61%. Intracellular, extracellular, and total A. pullulans FRR 5284 transfructosylating enzymes from different production systems resulted in different FOS profiles, indicating that FOS profiles can be controlled by adjusting intracellular and extracellular enzyme ratios and, hence prebiotic activity.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Molasses</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Transfructosylation</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fructooligosaccharides</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Nitrogen</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Scale-up</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hassanpour, Morteza</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Harrison, Mark D.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Speight, Robert E.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">O’Hara, Ian M.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhang, Zhanying</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Bioresources and Bioprocessing</subfield><subfield code="d">Berlin : SpringerOpen, 2014</subfield><subfield code="g">8(2021), 1 vom: 11. 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Highly efficient production of transfructosylating enzymes using low-cost sugarcane molasses by A. pullulans FRR 5284

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