Toxicological evaluation of the dried hydroethanolic extract of Amaranthus spinosus L. roots in Artemia salina larvae and Sprague Dawley rats
Background Amaranthus spinosus is a medicinal plant used in traditional medicine to treat several diseases including diabetes and its complications. The aim of this study was to prove the safety of the plant in animal health. Methods The dry extract was obtained following the hydroethanolic extracti...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Atchou, Kokou [verfasserIn] Lawson-Evi, Povi [verfasserIn] Diallo, Aboudoulatif [verfasserIn] Eklu-Gadegbeku, Kwashie [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2021 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s) 2021 |
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| Übergeordnetes Werk: |
Enthalten in: Clinical phytoscience - Heidelberg : SpringerOpen, 2015, 7(2021), 1 vom: 21. Juli |
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| Übergeordnetes Werk: |
volume:7 ; year:2021 ; number:1 ; day:21 ; month:07 |
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| DOI / URN: |
10.1186/s40816-021-00304-1 |
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| Katalog-ID: |
SPR044616279 |
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| 520 | |a Background Amaranthus spinosus is a medicinal plant used in traditional medicine to treat several diseases including diabetes and its complications. The aim of this study was to prove the safety of the plant in animal health. Methods The dry extract was obtained following the hydroethanolic extraction of A. spinosus roots. The cytotoxicity was evaluated in vitro by incubating Artemia salina larvae with the extract for 24 h. In vivo toxicity was assessed in Sprague Dawley rats. A single dose of 5000 mg/kg bw of extract was administered orally to female rats in acute toxicity and observed for 14 days for mortality and signs of toxicity. In subchronic toxicity, extract doses of 500 and 1000 mg/kg bw were administered orally to male and female rats for 28 consecutive days and observed for previous signs. Body weight was recorded daily and blood glucose levels every week. On day 29, blood was collected for biochemical and hematological studies. Organs were then exised for gross autopsy and histopathological examination. Results The in vitro study showed that the extract had a $ LC_{50} $ = 1.178 mg/mL in larvae and was considered to be non-cytotoxic. Oral administration of extract at a single dose of 5000 mg/kg bw did not cause any mortality or sign of toxicity in gross necropsy. In subchronic oral toxicity, repeated doses of 500 and 1000 mg/kg bw of extract, did not also cause any mortality or significant change in body weight, relative weight of vital organs. Furthermore, hematological and biochemical parameters and histopathological examination did not show any significant change. The observed decrease in blood glucose levels did not correlate with organ damage and supports the safety of the plant. However, the reduction of LDL-cholesterol has shown that the extract can prevent cardiovascular disease. Conclusions This finding demonstrated that A. spinosus root is non-toxic with a $ LD_{50} $ > 5000 mg/kg bw. Thus, the extract can be used for cutaneous and subchronic oral administration at doses ≤ 1000 mg/kg bw. However, further studies such as embryo/fetotoxicity, genotoxicity and neurotoxicity will be needed to prove the safety of chronic administration of the extract in patients and fetuses. | ||
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10.1186/s40816-021-00304-1 doi (DE-627)SPR044616279 (SPR)s40816-021-00304-1-e DE-627 ger DE-627 rakwb eng 630 ASE 44.41 bkl Atchou, Kokou verfasserin aut Toxicological evaluation of the dried hydroethanolic extract of Amaranthus spinosus L. roots in Artemia salina larvae and Sprague Dawley rats 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2021 Background Amaranthus spinosus is a medicinal plant used in traditional medicine to treat several diseases including diabetes and its complications. The aim of this study was to prove the safety of the plant in animal health. Methods The dry extract was obtained following the hydroethanolic extraction of A. spinosus roots. The cytotoxicity was evaluated in vitro by incubating Artemia salina larvae with the extract for 24 h. In vivo toxicity was assessed in Sprague Dawley rats. A single dose of 5000 mg/kg bw of extract was administered orally to female rats in acute toxicity and observed for 14 days for mortality and signs of toxicity. In subchronic toxicity, extract doses of 500 and 1000 mg/kg bw were administered orally to male and female rats for 28 consecutive days and observed for previous signs. Body weight was recorded daily and blood glucose levels every week. On day 29, blood was collected for biochemical and hematological studies. Organs were then exised for gross autopsy and histopathological examination. Results The in vitro study showed that the extract had a $ LC_{50} $ = 1.178 mg/mL in larvae and was considered to be non-cytotoxic. Oral administration of extract at a single dose of 5000 mg/kg bw did not cause any mortality or sign of toxicity in gross necropsy. In subchronic oral toxicity, repeated doses of 500 and 1000 mg/kg bw of extract, did not also cause any mortality or significant change in body weight, relative weight of vital organs. Furthermore, hematological and biochemical parameters and histopathological examination did not show any significant change. The observed decrease in blood glucose levels did not correlate with organ damage and supports the safety of the plant. However, the reduction of LDL-cholesterol has shown that the extract can prevent cardiovascular disease. Conclusions This finding demonstrated that A. spinosus root is non-toxic with a $ LD_{50} $ > 5000 mg/kg bw. Thus, the extract can be used for cutaneous and subchronic oral administration at doses ≤ 1000 mg/kg bw. However, further studies such as embryo/fetotoxicity, genotoxicity and neurotoxicity will be needed to prove the safety of chronic administration of the extract in patients and fetuses. cytotoxicity (dpeaa)DE-He213 acute toxicity (dpeaa)DE-He213 ATC method (dpeaa)DE-He213 subchronic toxicity (dpeaa)DE-He213 Lawson-Evi, Povi verfasserin aut Diallo, Aboudoulatif verfasserin aut Eklu-Gadegbeku, Kwashie verfasserin aut Enthalten in Clinical phytoscience Heidelberg : SpringerOpen, 2015 7(2021), 1 vom: 21. Juli (DE-627)835155773 (DE-600)2834057-7 2199-1197 nnns volume:7 year:2021 number:1 day:21 month:07 https://dx.doi.org/10.1186/s40816-021-00304-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.41 ASE AR 7 2021 1 21 07 |
| spelling |
10.1186/s40816-021-00304-1 doi (DE-627)SPR044616279 (SPR)s40816-021-00304-1-e DE-627 ger DE-627 rakwb eng 630 ASE 44.41 bkl Atchou, Kokou verfasserin aut Toxicological evaluation of the dried hydroethanolic extract of Amaranthus spinosus L. roots in Artemia salina larvae and Sprague Dawley rats 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2021 Background Amaranthus spinosus is a medicinal plant used in traditional medicine to treat several diseases including diabetes and its complications. The aim of this study was to prove the safety of the plant in animal health. Methods The dry extract was obtained following the hydroethanolic extraction of A. spinosus roots. The cytotoxicity was evaluated in vitro by incubating Artemia salina larvae with the extract for 24 h. In vivo toxicity was assessed in Sprague Dawley rats. A single dose of 5000 mg/kg bw of extract was administered orally to female rats in acute toxicity and observed for 14 days for mortality and signs of toxicity. In subchronic toxicity, extract doses of 500 and 1000 mg/kg bw were administered orally to male and female rats for 28 consecutive days and observed for previous signs. Body weight was recorded daily and blood glucose levels every week. On day 29, blood was collected for biochemical and hematological studies. Organs were then exised for gross autopsy and histopathological examination. Results The in vitro study showed that the extract had a $ LC_{50} $ = 1.178 mg/mL in larvae and was considered to be non-cytotoxic. Oral administration of extract at a single dose of 5000 mg/kg bw did not cause any mortality or sign of toxicity in gross necropsy. In subchronic oral toxicity, repeated doses of 500 and 1000 mg/kg bw of extract, did not also cause any mortality or significant change in body weight, relative weight of vital organs. Furthermore, hematological and biochemical parameters and histopathological examination did not show any significant change. The observed decrease in blood glucose levels did not correlate with organ damage and supports the safety of the plant. However, the reduction of LDL-cholesterol has shown that the extract can prevent cardiovascular disease. Conclusions This finding demonstrated that A. spinosus root is non-toxic with a $ LD_{50} $ > 5000 mg/kg bw. Thus, the extract can be used for cutaneous and subchronic oral administration at doses ≤ 1000 mg/kg bw. However, further studies such as embryo/fetotoxicity, genotoxicity and neurotoxicity will be needed to prove the safety of chronic administration of the extract in patients and fetuses. cytotoxicity (dpeaa)DE-He213 acute toxicity (dpeaa)DE-He213 ATC method (dpeaa)DE-He213 subchronic toxicity (dpeaa)DE-He213 Lawson-Evi, Povi verfasserin aut Diallo, Aboudoulatif verfasserin aut Eklu-Gadegbeku, Kwashie verfasserin aut Enthalten in Clinical phytoscience Heidelberg : SpringerOpen, 2015 7(2021), 1 vom: 21. Juli (DE-627)835155773 (DE-600)2834057-7 2199-1197 nnns volume:7 year:2021 number:1 day:21 month:07 https://dx.doi.org/10.1186/s40816-021-00304-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.41 ASE AR 7 2021 1 21 07 |
| allfields_unstemmed |
10.1186/s40816-021-00304-1 doi (DE-627)SPR044616279 (SPR)s40816-021-00304-1-e DE-627 ger DE-627 rakwb eng 630 ASE 44.41 bkl Atchou, Kokou verfasserin aut Toxicological evaluation of the dried hydroethanolic extract of Amaranthus spinosus L. roots in Artemia salina larvae and Sprague Dawley rats 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2021 Background Amaranthus spinosus is a medicinal plant used in traditional medicine to treat several diseases including diabetes and its complications. The aim of this study was to prove the safety of the plant in animal health. Methods The dry extract was obtained following the hydroethanolic extraction of A. spinosus roots. The cytotoxicity was evaluated in vitro by incubating Artemia salina larvae with the extract for 24 h. In vivo toxicity was assessed in Sprague Dawley rats. A single dose of 5000 mg/kg bw of extract was administered orally to female rats in acute toxicity and observed for 14 days for mortality and signs of toxicity. In subchronic toxicity, extract doses of 500 and 1000 mg/kg bw were administered orally to male and female rats for 28 consecutive days and observed for previous signs. Body weight was recorded daily and blood glucose levels every week. On day 29, blood was collected for biochemical and hematological studies. Organs were then exised for gross autopsy and histopathological examination. Results The in vitro study showed that the extract had a $ LC_{50} $ = 1.178 mg/mL in larvae and was considered to be non-cytotoxic. Oral administration of extract at a single dose of 5000 mg/kg bw did not cause any mortality or sign of toxicity in gross necropsy. In subchronic oral toxicity, repeated doses of 500 and 1000 mg/kg bw of extract, did not also cause any mortality or significant change in body weight, relative weight of vital organs. Furthermore, hematological and biochemical parameters and histopathological examination did not show any significant change. The observed decrease in blood glucose levels did not correlate with organ damage and supports the safety of the plant. However, the reduction of LDL-cholesterol has shown that the extract can prevent cardiovascular disease. Conclusions This finding demonstrated that A. spinosus root is non-toxic with a $ LD_{50} $ > 5000 mg/kg bw. Thus, the extract can be used for cutaneous and subchronic oral administration at doses ≤ 1000 mg/kg bw. However, further studies such as embryo/fetotoxicity, genotoxicity and neurotoxicity will be needed to prove the safety of chronic administration of the extract in patients and fetuses. cytotoxicity (dpeaa)DE-He213 acute toxicity (dpeaa)DE-He213 ATC method (dpeaa)DE-He213 subchronic toxicity (dpeaa)DE-He213 Lawson-Evi, Povi verfasserin aut Diallo, Aboudoulatif verfasserin aut Eklu-Gadegbeku, Kwashie verfasserin aut Enthalten in Clinical phytoscience Heidelberg : SpringerOpen, 2015 7(2021), 1 vom: 21. Juli (DE-627)835155773 (DE-600)2834057-7 2199-1197 nnns volume:7 year:2021 number:1 day:21 month:07 https://dx.doi.org/10.1186/s40816-021-00304-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.41 ASE AR 7 2021 1 21 07 |
| allfieldsGer |
10.1186/s40816-021-00304-1 doi (DE-627)SPR044616279 (SPR)s40816-021-00304-1-e DE-627 ger DE-627 rakwb eng 630 ASE 44.41 bkl Atchou, Kokou verfasserin aut Toxicological evaluation of the dried hydroethanolic extract of Amaranthus spinosus L. roots in Artemia salina larvae and Sprague Dawley rats 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2021 Background Amaranthus spinosus is a medicinal plant used in traditional medicine to treat several diseases including diabetes and its complications. The aim of this study was to prove the safety of the plant in animal health. Methods The dry extract was obtained following the hydroethanolic extraction of A. spinosus roots. The cytotoxicity was evaluated in vitro by incubating Artemia salina larvae with the extract for 24 h. In vivo toxicity was assessed in Sprague Dawley rats. A single dose of 5000 mg/kg bw of extract was administered orally to female rats in acute toxicity and observed for 14 days for mortality and signs of toxicity. In subchronic toxicity, extract doses of 500 and 1000 mg/kg bw were administered orally to male and female rats for 28 consecutive days and observed for previous signs. Body weight was recorded daily and blood glucose levels every week. On day 29, blood was collected for biochemical and hematological studies. Organs were then exised for gross autopsy and histopathological examination. Results The in vitro study showed that the extract had a $ LC_{50} $ = 1.178 mg/mL in larvae and was considered to be non-cytotoxic. Oral administration of extract at a single dose of 5000 mg/kg bw did not cause any mortality or sign of toxicity in gross necropsy. In subchronic oral toxicity, repeated doses of 500 and 1000 mg/kg bw of extract, did not also cause any mortality or significant change in body weight, relative weight of vital organs. Furthermore, hematological and biochemical parameters and histopathological examination did not show any significant change. The observed decrease in blood glucose levels did not correlate with organ damage and supports the safety of the plant. However, the reduction of LDL-cholesterol has shown that the extract can prevent cardiovascular disease. Conclusions This finding demonstrated that A. spinosus root is non-toxic with a $ LD_{50} $ > 5000 mg/kg bw. Thus, the extract can be used for cutaneous and subchronic oral administration at doses ≤ 1000 mg/kg bw. However, further studies such as embryo/fetotoxicity, genotoxicity and neurotoxicity will be needed to prove the safety of chronic administration of the extract in patients and fetuses. cytotoxicity (dpeaa)DE-He213 acute toxicity (dpeaa)DE-He213 ATC method (dpeaa)DE-He213 subchronic toxicity (dpeaa)DE-He213 Lawson-Evi, Povi verfasserin aut Diallo, Aboudoulatif verfasserin aut Eklu-Gadegbeku, Kwashie verfasserin aut Enthalten in Clinical phytoscience Heidelberg : SpringerOpen, 2015 7(2021), 1 vom: 21. Juli (DE-627)835155773 (DE-600)2834057-7 2199-1197 nnns volume:7 year:2021 number:1 day:21 month:07 https://dx.doi.org/10.1186/s40816-021-00304-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.41 ASE AR 7 2021 1 21 07 |
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10.1186/s40816-021-00304-1 doi (DE-627)SPR044616279 (SPR)s40816-021-00304-1-e DE-627 ger DE-627 rakwb eng 630 ASE 44.41 bkl Atchou, Kokou verfasserin aut Toxicological evaluation of the dried hydroethanolic extract of Amaranthus spinosus L. roots in Artemia salina larvae and Sprague Dawley rats 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2021 Background Amaranthus spinosus is a medicinal plant used in traditional medicine to treat several diseases including diabetes and its complications. The aim of this study was to prove the safety of the plant in animal health. Methods The dry extract was obtained following the hydroethanolic extraction of A. spinosus roots. The cytotoxicity was evaluated in vitro by incubating Artemia salina larvae with the extract for 24 h. In vivo toxicity was assessed in Sprague Dawley rats. A single dose of 5000 mg/kg bw of extract was administered orally to female rats in acute toxicity and observed for 14 days for mortality and signs of toxicity. In subchronic toxicity, extract doses of 500 and 1000 mg/kg bw were administered orally to male and female rats for 28 consecutive days and observed for previous signs. Body weight was recorded daily and blood glucose levels every week. On day 29, blood was collected for biochemical and hematological studies. Organs were then exised for gross autopsy and histopathological examination. Results The in vitro study showed that the extract had a $ LC_{50} $ = 1.178 mg/mL in larvae and was considered to be non-cytotoxic. Oral administration of extract at a single dose of 5000 mg/kg bw did not cause any mortality or sign of toxicity in gross necropsy. In subchronic oral toxicity, repeated doses of 500 and 1000 mg/kg bw of extract, did not also cause any mortality or significant change in body weight, relative weight of vital organs. Furthermore, hematological and biochemical parameters and histopathological examination did not show any significant change. The observed decrease in blood glucose levels did not correlate with organ damage and supports the safety of the plant. However, the reduction of LDL-cholesterol has shown that the extract can prevent cardiovascular disease. Conclusions This finding demonstrated that A. spinosus root is non-toxic with a $ LD_{50} $ > 5000 mg/kg bw. Thus, the extract can be used for cutaneous and subchronic oral administration at doses ≤ 1000 mg/kg bw. However, further studies such as embryo/fetotoxicity, genotoxicity and neurotoxicity will be needed to prove the safety of chronic administration of the extract in patients and fetuses. cytotoxicity (dpeaa)DE-He213 acute toxicity (dpeaa)DE-He213 ATC method (dpeaa)DE-He213 subchronic toxicity (dpeaa)DE-He213 Lawson-Evi, Povi verfasserin aut Diallo, Aboudoulatif verfasserin aut Eklu-Gadegbeku, Kwashie verfasserin aut Enthalten in Clinical phytoscience Heidelberg : SpringerOpen, 2015 7(2021), 1 vom: 21. Juli (DE-627)835155773 (DE-600)2834057-7 2199-1197 nnns volume:7 year:2021 number:1 day:21 month:07 https://dx.doi.org/10.1186/s40816-021-00304-1 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.41 ASE AR 7 2021 1 21 07 |
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toxicological evaluation of the dried hydroethanolic extract of amaranthus spinosus l. roots in artemia salina larvae and sprague dawley rats |
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Toxicological evaluation of the dried hydroethanolic extract of Amaranthus spinosus L. roots in Artemia salina larvae and Sprague Dawley rats |
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Background Amaranthus spinosus is a medicinal plant used in traditional medicine to treat several diseases including diabetes and its complications. The aim of this study was to prove the safety of the plant in animal health. Methods The dry extract was obtained following the hydroethanolic extraction of A. spinosus roots. The cytotoxicity was evaluated in vitro by incubating Artemia salina larvae with the extract for 24 h. In vivo toxicity was assessed in Sprague Dawley rats. A single dose of 5000 mg/kg bw of extract was administered orally to female rats in acute toxicity and observed for 14 days for mortality and signs of toxicity. In subchronic toxicity, extract doses of 500 and 1000 mg/kg bw were administered orally to male and female rats for 28 consecutive days and observed for previous signs. Body weight was recorded daily and blood glucose levels every week. On day 29, blood was collected for biochemical and hematological studies. Organs were then exised for gross autopsy and histopathological examination. Results The in vitro study showed that the extract had a $ LC_{50} $ = 1.178 mg/mL in larvae and was considered to be non-cytotoxic. Oral administration of extract at a single dose of 5000 mg/kg bw did not cause any mortality or sign of toxicity in gross necropsy. In subchronic oral toxicity, repeated doses of 500 and 1000 mg/kg bw of extract, did not also cause any mortality or significant change in body weight, relative weight of vital organs. Furthermore, hematological and biochemical parameters and histopathological examination did not show any significant change. The observed decrease in blood glucose levels did not correlate with organ damage and supports the safety of the plant. However, the reduction of LDL-cholesterol has shown that the extract can prevent cardiovascular disease. Conclusions This finding demonstrated that A. spinosus root is non-toxic with a $ LD_{50} $ > 5000 mg/kg bw. Thus, the extract can be used for cutaneous and subchronic oral administration at doses ≤ 1000 mg/kg bw. However, further studies such as embryo/fetotoxicity, genotoxicity and neurotoxicity will be needed to prove the safety of chronic administration of the extract in patients and fetuses. © The Author(s) 2021 |
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Background Amaranthus spinosus is a medicinal plant used in traditional medicine to treat several diseases including diabetes and its complications. The aim of this study was to prove the safety of the plant in animal health. Methods The dry extract was obtained following the hydroethanolic extraction of A. spinosus roots. The cytotoxicity was evaluated in vitro by incubating Artemia salina larvae with the extract for 24 h. In vivo toxicity was assessed in Sprague Dawley rats. A single dose of 5000 mg/kg bw of extract was administered orally to female rats in acute toxicity and observed for 14 days for mortality and signs of toxicity. In subchronic toxicity, extract doses of 500 and 1000 mg/kg bw were administered orally to male and female rats for 28 consecutive days and observed for previous signs. Body weight was recorded daily and blood glucose levels every week. On day 29, blood was collected for biochemical and hematological studies. Organs were then exised for gross autopsy and histopathological examination. Results The in vitro study showed that the extract had a $ LC_{50} $ = 1.178 mg/mL in larvae and was considered to be non-cytotoxic. Oral administration of extract at a single dose of 5000 mg/kg bw did not cause any mortality or sign of toxicity in gross necropsy. In subchronic oral toxicity, repeated doses of 500 and 1000 mg/kg bw of extract, did not also cause any mortality or significant change in body weight, relative weight of vital organs. Furthermore, hematological and biochemical parameters and histopathological examination did not show any significant change. The observed decrease in blood glucose levels did not correlate with organ damage and supports the safety of the plant. However, the reduction of LDL-cholesterol has shown that the extract can prevent cardiovascular disease. Conclusions This finding demonstrated that A. spinosus root is non-toxic with a $ LD_{50} $ > 5000 mg/kg bw. Thus, the extract can be used for cutaneous and subchronic oral administration at doses ≤ 1000 mg/kg bw. However, further studies such as embryo/fetotoxicity, genotoxicity and neurotoxicity will be needed to prove the safety of chronic administration of the extract in patients and fetuses. © The Author(s) 2021 |
| abstract_unstemmed |
Background Amaranthus spinosus is a medicinal plant used in traditional medicine to treat several diseases including diabetes and its complications. The aim of this study was to prove the safety of the plant in animal health. Methods The dry extract was obtained following the hydroethanolic extraction of A. spinosus roots. The cytotoxicity was evaluated in vitro by incubating Artemia salina larvae with the extract for 24 h. In vivo toxicity was assessed in Sprague Dawley rats. A single dose of 5000 mg/kg bw of extract was administered orally to female rats in acute toxicity and observed for 14 days for mortality and signs of toxicity. In subchronic toxicity, extract doses of 500 and 1000 mg/kg bw were administered orally to male and female rats for 28 consecutive days and observed for previous signs. Body weight was recorded daily and blood glucose levels every week. On day 29, blood was collected for biochemical and hematological studies. Organs were then exised for gross autopsy and histopathological examination. Results The in vitro study showed that the extract had a $ LC_{50} $ = 1.178 mg/mL in larvae and was considered to be non-cytotoxic. Oral administration of extract at a single dose of 5000 mg/kg bw did not cause any mortality or sign of toxicity in gross necropsy. In subchronic oral toxicity, repeated doses of 500 and 1000 mg/kg bw of extract, did not also cause any mortality or significant change in body weight, relative weight of vital organs. Furthermore, hematological and biochemical parameters and histopathological examination did not show any significant change. The observed decrease in blood glucose levels did not correlate with organ damage and supports the safety of the plant. However, the reduction of LDL-cholesterol has shown that the extract can prevent cardiovascular disease. Conclusions This finding demonstrated that A. spinosus root is non-toxic with a $ LD_{50} $ > 5000 mg/kg bw. Thus, the extract can be used for cutaneous and subchronic oral administration at doses ≤ 1000 mg/kg bw. However, further studies such as embryo/fetotoxicity, genotoxicity and neurotoxicity will be needed to prove the safety of chronic administration of the extract in patients and fetuses. © The Author(s) 2021 |
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Toxicological evaluation of the dried hydroethanolic extract of Amaranthus spinosus L. roots in Artemia salina larvae and Sprague Dawley rats |
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