Magnesium transport in the aglomerular kidney of the Gulf toadfish (Opsanus beta)
Abstract Despite having an aglomerular kidney, Gulf toadfish can survive in water ranging from nearly fresh up to 70 parts per thousand salinity. In hyperosmotic environments, the major renal function is to balance the passive $ Mg^{2+} $ load from the environment with an equal excretion. However, t...
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| Autor*in: |
Hansen, Nina G. Walker [verfasserIn] Madsen, Steffen S. [verfasserIn] Brauckhoff, Melanie [verfasserIn] Heuer, Rachael M. [verfasserIn] Schlenker, Lela S. [verfasserIn] Engelund, Morten B. [verfasserIn] Grosell, Martin [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2021 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of comparative physiology - Berlin : Springer, 1984, 191(2021), 5 vom: 23. Juli, Seite 865-880 |
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| Übergeordnetes Werk: |
volume:191 ; year:2021 ; number:5 ; day:23 ; month:07 ; pages:865-880 |
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| DOI / URN: |
10.1007/s00360-021-01392-8 |
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| Katalog-ID: |
SPR044893434 |
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| 100 | 1 | |a Hansen, Nina G. Walker |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Magnesium transport in the aglomerular kidney of the Gulf toadfish (Opsanus beta) |
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| 520 | |a Abstract Despite having an aglomerular kidney, Gulf toadfish can survive in water ranging from nearly fresh up to 70 parts per thousand salinity. In hyperosmotic environments, the major renal function is to balance the passive $ Mg^{2+} $ load from the environment with an equal excretion. However, the molecular transporters involved in $ Mg^{2+} $ secretion are poorly understood. We investigated whether environmental $ MgCl_{2} $ alone or in combination with elevated salinity affected transcriptional regulation of genes classically involved in renal $ Mg^{2+} $ secretion (slc41a1, slc41a3, cnnm3) together with three novel genes (trpm6, trpm7, claudin-19) and two isoforms of the $ Na^{+} $/$ K^{+} $-ATPase α-subunit (nka-α1a, nka-α1b). First, toadfish were acclimated to 5, 9, 35, or 60 ppt water (corresponding to ~ 7, 13, 50 and 108 mmol $ L^{−1} $ ambient [$ Mg^{2+} $], respectively) and sampled at 24 h or 9 days. Next, the impact of elevated ambient [$ Mg^{2+} $] was explored by exposing toadfish to control (50 mmol $ L^{−1} $ $ Mg^{2+} $), or elevated [$ Mg^{2+} $] (100 mmol $ L^{−1} $) at a constant salinity for 7 days. $ Mg^{2+} $ levels in this experiment corresponded with levels in control and hypersaline conditions in the first experiment. A salinity increase from 5 to 60 ppt stimulated the level of all investigated transcripts in the kidney. In $ Mg^{2+} $-exposed fish, we observed a 14-fold increase in the volume of intestinal fluids and elevated plasma osmolality and [$ Mg^{2+} $], suggesting osmoregulatory challenges. However, none of the renal gene targets changed expression compared with the control group. We conclude that transcriptional regulation of renal $ Mg^{2+} $ transporters is induced by elevated [$ Mg^{2+} $] in combination with salinity rather than elevated ambient [$ Mg^{2+} $] alone. | ||
| 650 | 4 | |a Aglomerular fish |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Homeostasis |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Salinity |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Kidney |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Tubular secretion |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Madsen, Steffen S. |e verfasserin |4 aut | |
| 700 | 1 | |a Brauckhoff, Melanie |e verfasserin |4 aut | |
| 700 | 1 | |a Heuer, Rachael M. |e verfasserin |4 aut | |
| 700 | 1 | |a Schlenker, Lela S. |e verfasserin |4 aut | |
| 700 | 1 | |a Engelund, Morten B. |e verfasserin |4 aut | |
| 700 | 1 | |a Grosell, Martin |e verfasserin |4 aut | |
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10.1007/s00360-021-01392-8 doi (DE-627)SPR044893434 (SPR)s00360-021-01392-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.37 bkl Hansen, Nina G. Walker verfasserin aut Magnesium transport in the aglomerular kidney of the Gulf toadfish (Opsanus beta) 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 Abstract Despite having an aglomerular kidney, Gulf toadfish can survive in water ranging from nearly fresh up to 70 parts per thousand salinity. In hyperosmotic environments, the major renal function is to balance the passive $ Mg^{2+} $ load from the environment with an equal excretion. However, the molecular transporters involved in $ Mg^{2+} $ secretion are poorly understood. We investigated whether environmental $ MgCl_{2} $ alone or in combination with elevated salinity affected transcriptional regulation of genes classically involved in renal $ Mg^{2+} $ secretion (slc41a1, slc41a3, cnnm3) together with three novel genes (trpm6, trpm7, claudin-19) and two isoforms of the $ Na^{+} $/$ K^{+} $-ATPase α-subunit (nka-α1a, nka-α1b). First, toadfish were acclimated to 5, 9, 35, or 60 ppt water (corresponding to ~ 7, 13, 50 and 108 mmol $ L^{−1} $ ambient [$ Mg^{2+} $], respectively) and sampled at 24 h or 9 days. Next, the impact of elevated ambient [$ Mg^{2+} $] was explored by exposing toadfish to control (50 mmol $ L^{−1} $ $ Mg^{2+} $), or elevated [$ Mg^{2+} $] (100 mmol $ L^{−1} $) at a constant salinity for 7 days. $ Mg^{2+} $ levels in this experiment corresponded with levels in control and hypersaline conditions in the first experiment. A salinity increase from 5 to 60 ppt stimulated the level of all investigated transcripts in the kidney. In $ Mg^{2+} $-exposed fish, we observed a 14-fold increase in the volume of intestinal fluids and elevated plasma osmolality and [$ Mg^{2+} $], suggesting osmoregulatory challenges. However, none of the renal gene targets changed expression compared with the control group. We conclude that transcriptional regulation of renal $ Mg^{2+} $ transporters is induced by elevated [$ Mg^{2+} $] in combination with salinity rather than elevated ambient [$ Mg^{2+} $] alone. Aglomerular fish (dpeaa)DE-He213 Homeostasis (dpeaa)DE-He213 Salinity (dpeaa)DE-He213 Kidney (dpeaa)DE-He213 Tubular secretion (dpeaa)DE-He213 Madsen, Steffen S. verfasserin aut Brauckhoff, Melanie verfasserin aut Heuer, Rachael M. verfasserin aut Schlenker, Lela S. verfasserin aut Engelund, Morten B. verfasserin aut Grosell, Martin verfasserin aut Enthalten in Journal of comparative physiology Berlin : Springer, 1984 191(2021), 5 vom: 23. Juli, Seite 865-880 (DE-627)25376968X (DE-600)1459302-6 1432-136X nnns volume:191 year:2021 number:5 day:23 month:07 pages:865-880 https://dx.doi.org/10.1007/s00360-021-01392-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.37 ASE AR 191 2021 5 23 07 865-880 |
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10.1007/s00360-021-01392-8 doi (DE-627)SPR044893434 (SPR)s00360-021-01392-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.37 bkl Hansen, Nina G. Walker verfasserin aut Magnesium transport in the aglomerular kidney of the Gulf toadfish (Opsanus beta) 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 Abstract Despite having an aglomerular kidney, Gulf toadfish can survive in water ranging from nearly fresh up to 70 parts per thousand salinity. In hyperosmotic environments, the major renal function is to balance the passive $ Mg^{2+} $ load from the environment with an equal excretion. However, the molecular transporters involved in $ Mg^{2+} $ secretion are poorly understood. We investigated whether environmental $ MgCl_{2} $ alone or in combination with elevated salinity affected transcriptional regulation of genes classically involved in renal $ Mg^{2+} $ secretion (slc41a1, slc41a3, cnnm3) together with three novel genes (trpm6, trpm7, claudin-19) and two isoforms of the $ Na^{+} $/$ K^{+} $-ATPase α-subunit (nka-α1a, nka-α1b). First, toadfish were acclimated to 5, 9, 35, or 60 ppt water (corresponding to ~ 7, 13, 50 and 108 mmol $ L^{−1} $ ambient [$ Mg^{2+} $], respectively) and sampled at 24 h or 9 days. Next, the impact of elevated ambient [$ Mg^{2+} $] was explored by exposing toadfish to control (50 mmol $ L^{−1} $ $ Mg^{2+} $), or elevated [$ Mg^{2+} $] (100 mmol $ L^{−1} $) at a constant salinity for 7 days. $ Mg^{2+} $ levels in this experiment corresponded with levels in control and hypersaline conditions in the first experiment. A salinity increase from 5 to 60 ppt stimulated the level of all investigated transcripts in the kidney. In $ Mg^{2+} $-exposed fish, we observed a 14-fold increase in the volume of intestinal fluids and elevated plasma osmolality and [$ Mg^{2+} $], suggesting osmoregulatory challenges. However, none of the renal gene targets changed expression compared with the control group. We conclude that transcriptional regulation of renal $ Mg^{2+} $ transporters is induced by elevated [$ Mg^{2+} $] in combination with salinity rather than elevated ambient [$ Mg^{2+} $] alone. Aglomerular fish (dpeaa)DE-He213 Homeostasis (dpeaa)DE-He213 Salinity (dpeaa)DE-He213 Kidney (dpeaa)DE-He213 Tubular secretion (dpeaa)DE-He213 Madsen, Steffen S. verfasserin aut Brauckhoff, Melanie verfasserin aut Heuer, Rachael M. verfasserin aut Schlenker, Lela S. verfasserin aut Engelund, Morten B. verfasserin aut Grosell, Martin verfasserin aut Enthalten in Journal of comparative physiology Berlin : Springer, 1984 191(2021), 5 vom: 23. Juli, Seite 865-880 (DE-627)25376968X (DE-600)1459302-6 1432-136X nnns volume:191 year:2021 number:5 day:23 month:07 pages:865-880 https://dx.doi.org/10.1007/s00360-021-01392-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.37 ASE AR 191 2021 5 23 07 865-880 |
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10.1007/s00360-021-01392-8 doi (DE-627)SPR044893434 (SPR)s00360-021-01392-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.37 bkl Hansen, Nina G. Walker verfasserin aut Magnesium transport in the aglomerular kidney of the Gulf toadfish (Opsanus beta) 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 Abstract Despite having an aglomerular kidney, Gulf toadfish can survive in water ranging from nearly fresh up to 70 parts per thousand salinity. In hyperosmotic environments, the major renal function is to balance the passive $ Mg^{2+} $ load from the environment with an equal excretion. However, the molecular transporters involved in $ Mg^{2+} $ secretion are poorly understood. We investigated whether environmental $ MgCl_{2} $ alone or in combination with elevated salinity affected transcriptional regulation of genes classically involved in renal $ Mg^{2+} $ secretion (slc41a1, slc41a3, cnnm3) together with three novel genes (trpm6, trpm7, claudin-19) and two isoforms of the $ Na^{+} $/$ K^{+} $-ATPase α-subunit (nka-α1a, nka-α1b). First, toadfish were acclimated to 5, 9, 35, or 60 ppt water (corresponding to ~ 7, 13, 50 and 108 mmol $ L^{−1} $ ambient [$ Mg^{2+} $], respectively) and sampled at 24 h or 9 days. Next, the impact of elevated ambient [$ Mg^{2+} $] was explored by exposing toadfish to control (50 mmol $ L^{−1} $ $ Mg^{2+} $), or elevated [$ Mg^{2+} $] (100 mmol $ L^{−1} $) at a constant salinity for 7 days. $ Mg^{2+} $ levels in this experiment corresponded with levels in control and hypersaline conditions in the first experiment. A salinity increase from 5 to 60 ppt stimulated the level of all investigated transcripts in the kidney. In $ Mg^{2+} $-exposed fish, we observed a 14-fold increase in the volume of intestinal fluids and elevated plasma osmolality and [$ Mg^{2+} $], suggesting osmoregulatory challenges. However, none of the renal gene targets changed expression compared with the control group. We conclude that transcriptional regulation of renal $ Mg^{2+} $ transporters is induced by elevated [$ Mg^{2+} $] in combination with salinity rather than elevated ambient [$ Mg^{2+} $] alone. Aglomerular fish (dpeaa)DE-He213 Homeostasis (dpeaa)DE-He213 Salinity (dpeaa)DE-He213 Kidney (dpeaa)DE-He213 Tubular secretion (dpeaa)DE-He213 Madsen, Steffen S. verfasserin aut Brauckhoff, Melanie verfasserin aut Heuer, Rachael M. verfasserin aut Schlenker, Lela S. verfasserin aut Engelund, Morten B. verfasserin aut Grosell, Martin verfasserin aut Enthalten in Journal of comparative physiology Berlin : Springer, 1984 191(2021), 5 vom: 23. Juli, Seite 865-880 (DE-627)25376968X (DE-600)1459302-6 1432-136X nnns volume:191 year:2021 number:5 day:23 month:07 pages:865-880 https://dx.doi.org/10.1007/s00360-021-01392-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.37 ASE AR 191 2021 5 23 07 865-880 |
| allfieldsGer |
10.1007/s00360-021-01392-8 doi (DE-627)SPR044893434 (SPR)s00360-021-01392-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.37 bkl Hansen, Nina G. Walker verfasserin aut Magnesium transport in the aglomerular kidney of the Gulf toadfish (Opsanus beta) 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 Abstract Despite having an aglomerular kidney, Gulf toadfish can survive in water ranging from nearly fresh up to 70 parts per thousand salinity. In hyperosmotic environments, the major renal function is to balance the passive $ Mg^{2+} $ load from the environment with an equal excretion. However, the molecular transporters involved in $ Mg^{2+} $ secretion are poorly understood. We investigated whether environmental $ MgCl_{2} $ alone or in combination with elevated salinity affected transcriptional regulation of genes classically involved in renal $ Mg^{2+} $ secretion (slc41a1, slc41a3, cnnm3) together with three novel genes (trpm6, trpm7, claudin-19) and two isoforms of the $ Na^{+} $/$ K^{+} $-ATPase α-subunit (nka-α1a, nka-α1b). First, toadfish were acclimated to 5, 9, 35, or 60 ppt water (corresponding to ~ 7, 13, 50 and 108 mmol $ L^{−1} $ ambient [$ Mg^{2+} $], respectively) and sampled at 24 h or 9 days. Next, the impact of elevated ambient [$ Mg^{2+} $] was explored by exposing toadfish to control (50 mmol $ L^{−1} $ $ Mg^{2+} $), or elevated [$ Mg^{2+} $] (100 mmol $ L^{−1} $) at a constant salinity for 7 days. $ Mg^{2+} $ levels in this experiment corresponded with levels in control and hypersaline conditions in the first experiment. A salinity increase from 5 to 60 ppt stimulated the level of all investigated transcripts in the kidney. In $ Mg^{2+} $-exposed fish, we observed a 14-fold increase in the volume of intestinal fluids and elevated plasma osmolality and [$ Mg^{2+} $], suggesting osmoregulatory challenges. However, none of the renal gene targets changed expression compared with the control group. We conclude that transcriptional regulation of renal $ Mg^{2+} $ transporters is induced by elevated [$ Mg^{2+} $] in combination with salinity rather than elevated ambient [$ Mg^{2+} $] alone. Aglomerular fish (dpeaa)DE-He213 Homeostasis (dpeaa)DE-He213 Salinity (dpeaa)DE-He213 Kidney (dpeaa)DE-He213 Tubular secretion (dpeaa)DE-He213 Madsen, Steffen S. verfasserin aut Brauckhoff, Melanie verfasserin aut Heuer, Rachael M. verfasserin aut Schlenker, Lela S. verfasserin aut Engelund, Morten B. verfasserin aut Grosell, Martin verfasserin aut Enthalten in Journal of comparative physiology Berlin : Springer, 1984 191(2021), 5 vom: 23. Juli, Seite 865-880 (DE-627)25376968X (DE-600)1459302-6 1432-136X nnns volume:191 year:2021 number:5 day:23 month:07 pages:865-880 https://dx.doi.org/10.1007/s00360-021-01392-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.37 ASE AR 191 2021 5 23 07 865-880 |
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10.1007/s00360-021-01392-8 doi (DE-627)SPR044893434 (SPR)s00360-021-01392-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.37 bkl Hansen, Nina G. Walker verfasserin aut Magnesium transport in the aglomerular kidney of the Gulf toadfish (Opsanus beta) 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 Abstract Despite having an aglomerular kidney, Gulf toadfish can survive in water ranging from nearly fresh up to 70 parts per thousand salinity. In hyperosmotic environments, the major renal function is to balance the passive $ Mg^{2+} $ load from the environment with an equal excretion. However, the molecular transporters involved in $ Mg^{2+} $ secretion are poorly understood. We investigated whether environmental $ MgCl_{2} $ alone or in combination with elevated salinity affected transcriptional regulation of genes classically involved in renal $ Mg^{2+} $ secretion (slc41a1, slc41a3, cnnm3) together with three novel genes (trpm6, trpm7, claudin-19) and two isoforms of the $ Na^{+} $/$ K^{+} $-ATPase α-subunit (nka-α1a, nka-α1b). First, toadfish were acclimated to 5, 9, 35, or 60 ppt water (corresponding to ~ 7, 13, 50 and 108 mmol $ L^{−1} $ ambient [$ Mg^{2+} $], respectively) and sampled at 24 h or 9 days. Next, the impact of elevated ambient [$ Mg^{2+} $] was explored by exposing toadfish to control (50 mmol $ L^{−1} $ $ Mg^{2+} $), or elevated [$ Mg^{2+} $] (100 mmol $ L^{−1} $) at a constant salinity for 7 days. $ Mg^{2+} $ levels in this experiment corresponded with levels in control and hypersaline conditions in the first experiment. A salinity increase from 5 to 60 ppt stimulated the level of all investigated transcripts in the kidney. In $ Mg^{2+} $-exposed fish, we observed a 14-fold increase in the volume of intestinal fluids and elevated plasma osmolality and [$ Mg^{2+} $], suggesting osmoregulatory challenges. However, none of the renal gene targets changed expression compared with the control group. We conclude that transcriptional regulation of renal $ Mg^{2+} $ transporters is induced by elevated [$ Mg^{2+} $] in combination with salinity rather than elevated ambient [$ Mg^{2+} $] alone. Aglomerular fish (dpeaa)DE-He213 Homeostasis (dpeaa)DE-He213 Salinity (dpeaa)DE-He213 Kidney (dpeaa)DE-He213 Tubular secretion (dpeaa)DE-He213 Madsen, Steffen S. verfasserin aut Brauckhoff, Melanie verfasserin aut Heuer, Rachael M. verfasserin aut Schlenker, Lela S. verfasserin aut Engelund, Morten B. verfasserin aut Grosell, Martin verfasserin aut Enthalten in Journal of comparative physiology Berlin : Springer, 1984 191(2021), 5 vom: 23. Juli, Seite 865-880 (DE-627)25376968X (DE-600)1459302-6 1432-136X nnns volume:191 year:2021 number:5 day:23 month:07 pages:865-880 https://dx.doi.org/10.1007/s00360-021-01392-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.37 ASE AR 191 2021 5 23 07 865-880 |
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Hansen, Nina G. Walker @@aut@@ Madsen, Steffen S. @@aut@@ Brauckhoff, Melanie @@aut@@ Heuer, Rachael M. @@aut@@ Schlenker, Lela S. @@aut@@ Engelund, Morten B. @@aut@@ Grosell, Martin @@aut@@ |
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Hansen, Nina G. Walker |
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Hansen, Nina G. Walker ddc 610 bkl 44.37 misc Aglomerular fish misc Homeostasis misc Salinity misc Kidney misc Tubular secretion Magnesium transport in the aglomerular kidney of the Gulf toadfish (Opsanus beta) |
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610 ASE 44.37 bkl Magnesium transport in the aglomerular kidney of the Gulf toadfish (Opsanus beta) Aglomerular fish (dpeaa)DE-He213 Homeostasis (dpeaa)DE-He213 Salinity (dpeaa)DE-He213 Kidney (dpeaa)DE-He213 Tubular secretion (dpeaa)DE-He213 |
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Hansen, Nina G. Walker Madsen, Steffen S. Brauckhoff, Melanie Heuer, Rachael M. Schlenker, Lela S. Engelund, Morten B. Grosell, Martin |
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magnesium transport in the aglomerular kidney of the gulf toadfish (opsanus beta) |
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Magnesium transport in the aglomerular kidney of the Gulf toadfish (Opsanus beta) |
| abstract |
Abstract Despite having an aglomerular kidney, Gulf toadfish can survive in water ranging from nearly fresh up to 70 parts per thousand salinity. In hyperosmotic environments, the major renal function is to balance the passive $ Mg^{2+} $ load from the environment with an equal excretion. However, the molecular transporters involved in $ Mg^{2+} $ secretion are poorly understood. We investigated whether environmental $ MgCl_{2} $ alone or in combination with elevated salinity affected transcriptional regulation of genes classically involved in renal $ Mg^{2+} $ secretion (slc41a1, slc41a3, cnnm3) together with three novel genes (trpm6, trpm7, claudin-19) and two isoforms of the $ Na^{+} $/$ K^{+} $-ATPase α-subunit (nka-α1a, nka-α1b). First, toadfish were acclimated to 5, 9, 35, or 60 ppt water (corresponding to ~ 7, 13, 50 and 108 mmol $ L^{−1} $ ambient [$ Mg^{2+} $], respectively) and sampled at 24 h or 9 days. Next, the impact of elevated ambient [$ Mg^{2+} $] was explored by exposing toadfish to control (50 mmol $ L^{−1} $ $ Mg^{2+} $), or elevated [$ Mg^{2+} $] (100 mmol $ L^{−1} $) at a constant salinity for 7 days. $ Mg^{2+} $ levels in this experiment corresponded with levels in control and hypersaline conditions in the first experiment. A salinity increase from 5 to 60 ppt stimulated the level of all investigated transcripts in the kidney. In $ Mg^{2+} $-exposed fish, we observed a 14-fold increase in the volume of intestinal fluids and elevated plasma osmolality and [$ Mg^{2+} $], suggesting osmoregulatory challenges. However, none of the renal gene targets changed expression compared with the control group. We conclude that transcriptional regulation of renal $ Mg^{2+} $ transporters is induced by elevated [$ Mg^{2+} $] in combination with salinity rather than elevated ambient [$ Mg^{2+} $] alone. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 |
| abstractGer |
Abstract Despite having an aglomerular kidney, Gulf toadfish can survive in water ranging from nearly fresh up to 70 parts per thousand salinity. In hyperosmotic environments, the major renal function is to balance the passive $ Mg^{2+} $ load from the environment with an equal excretion. However, the molecular transporters involved in $ Mg^{2+} $ secretion are poorly understood. We investigated whether environmental $ MgCl_{2} $ alone or in combination with elevated salinity affected transcriptional regulation of genes classically involved in renal $ Mg^{2+} $ secretion (slc41a1, slc41a3, cnnm3) together with three novel genes (trpm6, trpm7, claudin-19) and two isoforms of the $ Na^{+} $/$ K^{+} $-ATPase α-subunit (nka-α1a, nka-α1b). First, toadfish were acclimated to 5, 9, 35, or 60 ppt water (corresponding to ~ 7, 13, 50 and 108 mmol $ L^{−1} $ ambient [$ Mg^{2+} $], respectively) and sampled at 24 h or 9 days. Next, the impact of elevated ambient [$ Mg^{2+} $] was explored by exposing toadfish to control (50 mmol $ L^{−1} $ $ Mg^{2+} $), or elevated [$ Mg^{2+} $] (100 mmol $ L^{−1} $) at a constant salinity for 7 days. $ Mg^{2+} $ levels in this experiment corresponded with levels in control and hypersaline conditions in the first experiment. A salinity increase from 5 to 60 ppt stimulated the level of all investigated transcripts in the kidney. In $ Mg^{2+} $-exposed fish, we observed a 14-fold increase in the volume of intestinal fluids and elevated plasma osmolality and [$ Mg^{2+} $], suggesting osmoregulatory challenges. However, none of the renal gene targets changed expression compared with the control group. We conclude that transcriptional regulation of renal $ Mg^{2+} $ transporters is induced by elevated [$ Mg^{2+} $] in combination with salinity rather than elevated ambient [$ Mg^{2+} $] alone. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 |
| abstract_unstemmed |
Abstract Despite having an aglomerular kidney, Gulf toadfish can survive in water ranging from nearly fresh up to 70 parts per thousand salinity. In hyperosmotic environments, the major renal function is to balance the passive $ Mg^{2+} $ load from the environment with an equal excretion. However, the molecular transporters involved in $ Mg^{2+} $ secretion are poorly understood. We investigated whether environmental $ MgCl_{2} $ alone or in combination with elevated salinity affected transcriptional regulation of genes classically involved in renal $ Mg^{2+} $ secretion (slc41a1, slc41a3, cnnm3) together with three novel genes (trpm6, trpm7, claudin-19) and two isoforms of the $ Na^{+} $/$ K^{+} $-ATPase α-subunit (nka-α1a, nka-α1b). First, toadfish were acclimated to 5, 9, 35, or 60 ppt water (corresponding to ~ 7, 13, 50 and 108 mmol $ L^{−1} $ ambient [$ Mg^{2+} $], respectively) and sampled at 24 h or 9 days. Next, the impact of elevated ambient [$ Mg^{2+} $] was explored by exposing toadfish to control (50 mmol $ L^{−1} $ $ Mg^{2+} $), or elevated [$ Mg^{2+} $] (100 mmol $ L^{−1} $) at a constant salinity for 7 days. $ Mg^{2+} $ levels in this experiment corresponded with levels in control and hypersaline conditions in the first experiment. A salinity increase from 5 to 60 ppt stimulated the level of all investigated transcripts in the kidney. In $ Mg^{2+} $-exposed fish, we observed a 14-fold increase in the volume of intestinal fluids and elevated plasma osmolality and [$ Mg^{2+} $], suggesting osmoregulatory challenges. However, none of the renal gene targets changed expression compared with the control group. We conclude that transcriptional regulation of renal $ Mg^{2+} $ transporters is induced by elevated [$ Mg^{2+} $] in combination with salinity rather than elevated ambient [$ Mg^{2+} $] alone. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR044893434</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519222645.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">210823s2021 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00360-021-01392-8</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR044893434</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s00360-021-01392-8-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">44.37</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Hansen, Nina G. Walker</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Magnesium transport in the aglomerular kidney of the Gulf toadfish (Opsanus beta)</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2021</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Despite having an aglomerular kidney, Gulf toadfish can survive in water ranging from nearly fresh up to 70 parts per thousand salinity. In hyperosmotic environments, the major renal function is to balance the passive $ Mg^{2+} $ load from the environment with an equal excretion. However, the molecular transporters involved in $ Mg^{2+} $ secretion are poorly understood. We investigated whether environmental $ MgCl_{2} $ alone or in combination with elevated salinity affected transcriptional regulation of genes classically involved in renal $ Mg^{2+} $ secretion (slc41a1, slc41a3, cnnm3) together with three novel genes (trpm6, trpm7, claudin-19) and two isoforms of the $ Na^{+} $/$ K^{+} $-ATPase α-subunit (nka-α1a, nka-α1b). First, toadfish were acclimated to 5, 9, 35, or 60 ppt water (corresponding to ~ 7, 13, 50 and 108 mmol $ L^{−1} $ ambient [$ Mg^{2+} $], respectively) and sampled at 24 h or 9 days. Next, the impact of elevated ambient [$ Mg^{2+} $] was explored by exposing toadfish to control (50 mmol $ L^{−1} $ $ Mg^{2+} $), or elevated [$ Mg^{2+} $] (100 mmol $ L^{−1} $) at a constant salinity for 7 days. $ Mg^{2+} $ levels in this experiment corresponded with levels in control and hypersaline conditions in the first experiment. A salinity increase from 5 to 60 ppt stimulated the level of all investigated transcripts in the kidney. In $ Mg^{2+} $-exposed fish, we observed a 14-fold increase in the volume of intestinal fluids and elevated plasma osmolality and [$ Mg^{2+} $], suggesting osmoregulatory challenges. However, none of the renal gene targets changed expression compared with the control group. We conclude that transcriptional regulation of renal $ Mg^{2+} $ transporters is induced by elevated [$ Mg^{2+} $] in combination with salinity rather than elevated ambient [$ Mg^{2+} $] alone.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Aglomerular fish</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Homeostasis</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Salinity</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Kidney</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Tubular secretion</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Madsen, Steffen S.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Brauckhoff, Melanie</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Heuer, Rachael M.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Schlenker, Lela S.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Engelund, Morten B.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Grosell, Martin</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Journal of comparative physiology</subfield><subfield code="d">Berlin : Springer, 1984</subfield><subfield code="g">191(2021), 5 vom: 23. 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