Uptake and phototoxicity of meso-tetrahydroxyphenyl chlorine are highly variable in human biliary tract cancer cell lines and correlate with markers of differentiation and proliferation
Abstract Photodynamic therapy (PDT) using Photofrin® and, recently, Foscan® has gained broad acceptance for palliative treatment of non-resectable cholangiocarcinoma (CC). No information, however, is available whether the phenotype of CC tumour cells has an effect on the efficiency of the treatment....
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Kiesslich, Tobias [verfasserIn] Neureiter, Daniel [verfasserIn] Alinger, Beate [verfasserIn] Jansky, Gerhard L. [verfasserIn] Berlanda, Juergen [verfasserIn] Mkrtchyan, Vahagn [verfasserIn] Ocker, Matthias [verfasserIn] Plaetzer, Kristjan [verfasserIn] Berr, Frieder [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2010 |
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| Anmerkung: |
© The Royal Society of Chemistry and Owner Societies 2010 |
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| Übergeordnetes Werk: |
Enthalten in: Photochemical & photobiological sciences - Heidelberg : Springer, 2002, 9(2010), 5 vom: 01. Apr., Seite 734-743 |
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| Übergeordnetes Werk: |
volume:9 ; year:2010 ; number:5 ; day:01 ; month:04 ; pages:734-743 |
| Links: |
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| DOI / URN: |
10.1039/b9pp00201d |
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| Katalog-ID: |
SPR045045631 |
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| 245 | 1 | 0 | |a Uptake and phototoxicity of meso-tetrahydroxyphenyl chlorine are highly variable in human biliary tract cancer cell lines and correlate with markers of differentiation and proliferation |
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| 520 | |a Abstract Photodynamic therapy (PDT) using Photofrin® and, recently, Foscan® has gained broad acceptance for palliative treatment of non-resectable cholangiocarcinoma (CC). No information, however, is available whether the phenotype of CC tumour cells has an effect on the efficiency of the treatment. Using a well-characterised set of n = 9 biliary tract cancer cell lines this study investigated the uptake, phototoxicity, and release of meso-tetrahydroxyphenyl chlorine (mTHPC, Foscan®) after incubation with 200 or 400 ng $ ml^{−1} $ mTHPC. For uptake of mTHPC we found great variations between the individual cell lines (up to a factor 2), resulting in even more pronounced differences in phototoxicity. Based on statistical classification by hierarchical cluster analysis, two groups of cell lines can be distinguished which are characterised by either high or low susceptibility towards mTHPC-based photodynamic treatment. Correlation analysis with previously established immunochemical parameters showed that cells with a low cytokeratin-19 (ductal differentiation), high vimentin (mesenchymal marker), and high proliferative phenotype preferentially show higher uptake of mTHPC and subsequent phototoxicity. These results demonstrate high variability of biliary tract cancer cells when subjected to mTHPC-based photodynamic treatment and identify possible markers that could be used in the clinical setting in order to predict the efficiency of PDT and adjust the dose for complete tumour elimination. | ||
| 700 | 1 | |a Neureiter, Daniel |e verfasserin |4 aut | |
| 700 | 1 | |a Alinger, Beate |e verfasserin |4 aut | |
| 700 | 1 | |a Jansky, Gerhard L. |e verfasserin |4 aut | |
| 700 | 1 | |a Berlanda, Juergen |e verfasserin |4 aut | |
| 700 | 1 | |a Mkrtchyan, Vahagn |e verfasserin |4 aut | |
| 700 | 1 | |a Ocker, Matthias |e verfasserin |4 aut | |
| 700 | 1 | |a Plaetzer, Kristjan |e verfasserin |4 aut | |
| 700 | 1 | |a Berr, Frieder |e verfasserin |4 aut | |
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10.1039/b9pp00201d doi (DE-627)SPR045045631 (SPR)b9pp00201d-e DE-627 ger DE-627 rakwb eng 620 ASE 35.16 bkl 42.14 bkl Kiesslich, Tobias verfasserin aut Uptake and phototoxicity of meso-tetrahydroxyphenyl chlorine are highly variable in human biliary tract cancer cell lines and correlate with markers of differentiation and proliferation 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Royal Society of Chemistry and Owner Societies 2010 Abstract Photodynamic therapy (PDT) using Photofrin® and, recently, Foscan® has gained broad acceptance for palliative treatment of non-resectable cholangiocarcinoma (CC). No information, however, is available whether the phenotype of CC tumour cells has an effect on the efficiency of the treatment. Using a well-characterised set of n = 9 biliary tract cancer cell lines this study investigated the uptake, phototoxicity, and release of meso-tetrahydroxyphenyl chlorine (mTHPC, Foscan®) after incubation with 200 or 400 ng $ ml^{−1} $ mTHPC. For uptake of mTHPC we found great variations between the individual cell lines (up to a factor 2), resulting in even more pronounced differences in phototoxicity. Based on statistical classification by hierarchical cluster analysis, two groups of cell lines can be distinguished which are characterised by either high or low susceptibility towards mTHPC-based photodynamic treatment. Correlation analysis with previously established immunochemical parameters showed that cells with a low cytokeratin-19 (ductal differentiation), high vimentin (mesenchymal marker), and high proliferative phenotype preferentially show higher uptake of mTHPC and subsequent phototoxicity. These results demonstrate high variability of biliary tract cancer cells when subjected to mTHPC-based photodynamic treatment and identify possible markers that could be used in the clinical setting in order to predict the efficiency of PDT and adjust the dose for complete tumour elimination. Neureiter, Daniel verfasserin aut Alinger, Beate verfasserin aut Jansky, Gerhard L. verfasserin aut Berlanda, Juergen verfasserin aut Mkrtchyan, Vahagn verfasserin aut Ocker, Matthias verfasserin aut Plaetzer, Kristjan verfasserin aut Berr, Frieder verfasserin aut Enthalten in Photochemical & photobiological sciences Heidelberg : Springer, 2002 9(2010), 5 vom: 01. Apr., Seite 734-743 (DE-627)342893742 (DE-600)2072584-X 1474-9092 nnns volume:9 year:2010 number:5 day:01 month:04 pages:734-743 https://dx.doi.org/10.1039/b9pp00201d lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2093 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2472 GBV_ILN_2522 GBV_ILN_2812 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4700 GBV_ILN_4753 35.16 ASE 42.14 ASE AR 9 2010 5 01 04 734-743 |
| spelling |
10.1039/b9pp00201d doi (DE-627)SPR045045631 (SPR)b9pp00201d-e DE-627 ger DE-627 rakwb eng 620 ASE 35.16 bkl 42.14 bkl Kiesslich, Tobias verfasserin aut Uptake and phototoxicity of meso-tetrahydroxyphenyl chlorine are highly variable in human biliary tract cancer cell lines and correlate with markers of differentiation and proliferation 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Royal Society of Chemistry and Owner Societies 2010 Abstract Photodynamic therapy (PDT) using Photofrin® and, recently, Foscan® has gained broad acceptance for palliative treatment of non-resectable cholangiocarcinoma (CC). No information, however, is available whether the phenotype of CC tumour cells has an effect on the efficiency of the treatment. Using a well-characterised set of n = 9 biliary tract cancer cell lines this study investigated the uptake, phototoxicity, and release of meso-tetrahydroxyphenyl chlorine (mTHPC, Foscan®) after incubation with 200 or 400 ng $ ml^{−1} $ mTHPC. For uptake of mTHPC we found great variations between the individual cell lines (up to a factor 2), resulting in even more pronounced differences in phototoxicity. Based on statistical classification by hierarchical cluster analysis, two groups of cell lines can be distinguished which are characterised by either high or low susceptibility towards mTHPC-based photodynamic treatment. Correlation analysis with previously established immunochemical parameters showed that cells with a low cytokeratin-19 (ductal differentiation), high vimentin (mesenchymal marker), and high proliferative phenotype preferentially show higher uptake of mTHPC and subsequent phototoxicity. These results demonstrate high variability of biliary tract cancer cells when subjected to mTHPC-based photodynamic treatment and identify possible markers that could be used in the clinical setting in order to predict the efficiency of PDT and adjust the dose for complete tumour elimination. Neureiter, Daniel verfasserin aut Alinger, Beate verfasserin aut Jansky, Gerhard L. verfasserin aut Berlanda, Juergen verfasserin aut Mkrtchyan, Vahagn verfasserin aut Ocker, Matthias verfasserin aut Plaetzer, Kristjan verfasserin aut Berr, Frieder verfasserin aut Enthalten in Photochemical & photobiological sciences Heidelberg : Springer, 2002 9(2010), 5 vom: 01. Apr., Seite 734-743 (DE-627)342893742 (DE-600)2072584-X 1474-9092 nnns volume:9 year:2010 number:5 day:01 month:04 pages:734-743 https://dx.doi.org/10.1039/b9pp00201d lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2093 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2472 GBV_ILN_2522 GBV_ILN_2812 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4700 GBV_ILN_4753 35.16 ASE 42.14 ASE AR 9 2010 5 01 04 734-743 |
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10.1039/b9pp00201d doi (DE-627)SPR045045631 (SPR)b9pp00201d-e DE-627 ger DE-627 rakwb eng 620 ASE 35.16 bkl 42.14 bkl Kiesslich, Tobias verfasserin aut Uptake and phototoxicity of meso-tetrahydroxyphenyl chlorine are highly variable in human biliary tract cancer cell lines and correlate with markers of differentiation and proliferation 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Royal Society of Chemistry and Owner Societies 2010 Abstract Photodynamic therapy (PDT) using Photofrin® and, recently, Foscan® has gained broad acceptance for palliative treatment of non-resectable cholangiocarcinoma (CC). No information, however, is available whether the phenotype of CC tumour cells has an effect on the efficiency of the treatment. Using a well-characterised set of n = 9 biliary tract cancer cell lines this study investigated the uptake, phototoxicity, and release of meso-tetrahydroxyphenyl chlorine (mTHPC, Foscan®) after incubation with 200 or 400 ng $ ml^{−1} $ mTHPC. For uptake of mTHPC we found great variations between the individual cell lines (up to a factor 2), resulting in even more pronounced differences in phototoxicity. Based on statistical classification by hierarchical cluster analysis, two groups of cell lines can be distinguished which are characterised by either high or low susceptibility towards mTHPC-based photodynamic treatment. Correlation analysis with previously established immunochemical parameters showed that cells with a low cytokeratin-19 (ductal differentiation), high vimentin (mesenchymal marker), and high proliferative phenotype preferentially show higher uptake of mTHPC and subsequent phototoxicity. These results demonstrate high variability of biliary tract cancer cells when subjected to mTHPC-based photodynamic treatment and identify possible markers that could be used in the clinical setting in order to predict the efficiency of PDT and adjust the dose for complete tumour elimination. Neureiter, Daniel verfasserin aut Alinger, Beate verfasserin aut Jansky, Gerhard L. verfasserin aut Berlanda, Juergen verfasserin aut Mkrtchyan, Vahagn verfasserin aut Ocker, Matthias verfasserin aut Plaetzer, Kristjan verfasserin aut Berr, Frieder verfasserin aut Enthalten in Photochemical & photobiological sciences Heidelberg : Springer, 2002 9(2010), 5 vom: 01. Apr., Seite 734-743 (DE-627)342893742 (DE-600)2072584-X 1474-9092 nnns volume:9 year:2010 number:5 day:01 month:04 pages:734-743 https://dx.doi.org/10.1039/b9pp00201d lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2093 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2472 GBV_ILN_2522 GBV_ILN_2812 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4700 GBV_ILN_4753 35.16 ASE 42.14 ASE AR 9 2010 5 01 04 734-743 |
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10.1039/b9pp00201d doi (DE-627)SPR045045631 (SPR)b9pp00201d-e DE-627 ger DE-627 rakwb eng 620 ASE 35.16 bkl 42.14 bkl Kiesslich, Tobias verfasserin aut Uptake and phototoxicity of meso-tetrahydroxyphenyl chlorine are highly variable in human biliary tract cancer cell lines and correlate with markers of differentiation and proliferation 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Royal Society of Chemistry and Owner Societies 2010 Abstract Photodynamic therapy (PDT) using Photofrin® and, recently, Foscan® has gained broad acceptance for palliative treatment of non-resectable cholangiocarcinoma (CC). No information, however, is available whether the phenotype of CC tumour cells has an effect on the efficiency of the treatment. Using a well-characterised set of n = 9 biliary tract cancer cell lines this study investigated the uptake, phototoxicity, and release of meso-tetrahydroxyphenyl chlorine (mTHPC, Foscan®) after incubation with 200 or 400 ng $ ml^{−1} $ mTHPC. For uptake of mTHPC we found great variations between the individual cell lines (up to a factor 2), resulting in even more pronounced differences in phototoxicity. Based on statistical classification by hierarchical cluster analysis, two groups of cell lines can be distinguished which are characterised by either high or low susceptibility towards mTHPC-based photodynamic treatment. Correlation analysis with previously established immunochemical parameters showed that cells with a low cytokeratin-19 (ductal differentiation), high vimentin (mesenchymal marker), and high proliferative phenotype preferentially show higher uptake of mTHPC and subsequent phototoxicity. These results demonstrate high variability of biliary tract cancer cells when subjected to mTHPC-based photodynamic treatment and identify possible markers that could be used in the clinical setting in order to predict the efficiency of PDT and adjust the dose for complete tumour elimination. Neureiter, Daniel verfasserin aut Alinger, Beate verfasserin aut Jansky, Gerhard L. verfasserin aut Berlanda, Juergen verfasserin aut Mkrtchyan, Vahagn verfasserin aut Ocker, Matthias verfasserin aut Plaetzer, Kristjan verfasserin aut Berr, Frieder verfasserin aut Enthalten in Photochemical & photobiological sciences Heidelberg : Springer, 2002 9(2010), 5 vom: 01. Apr., Seite 734-743 (DE-627)342893742 (DE-600)2072584-X 1474-9092 nnns volume:9 year:2010 number:5 day:01 month:04 pages:734-743 https://dx.doi.org/10.1039/b9pp00201d lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2093 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2472 GBV_ILN_2522 GBV_ILN_2812 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4700 GBV_ILN_4753 35.16 ASE 42.14 ASE AR 9 2010 5 01 04 734-743 |
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10.1039/b9pp00201d doi (DE-627)SPR045045631 (SPR)b9pp00201d-e DE-627 ger DE-627 rakwb eng 620 ASE 35.16 bkl 42.14 bkl Kiesslich, Tobias verfasserin aut Uptake and phototoxicity of meso-tetrahydroxyphenyl chlorine are highly variable in human biliary tract cancer cell lines and correlate with markers of differentiation and proliferation 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Royal Society of Chemistry and Owner Societies 2010 Abstract Photodynamic therapy (PDT) using Photofrin® and, recently, Foscan® has gained broad acceptance for palliative treatment of non-resectable cholangiocarcinoma (CC). No information, however, is available whether the phenotype of CC tumour cells has an effect on the efficiency of the treatment. Using a well-characterised set of n = 9 biliary tract cancer cell lines this study investigated the uptake, phototoxicity, and release of meso-tetrahydroxyphenyl chlorine (mTHPC, Foscan®) after incubation with 200 or 400 ng $ ml^{−1} $ mTHPC. For uptake of mTHPC we found great variations between the individual cell lines (up to a factor 2), resulting in even more pronounced differences in phototoxicity. Based on statistical classification by hierarchical cluster analysis, two groups of cell lines can be distinguished which are characterised by either high or low susceptibility towards mTHPC-based photodynamic treatment. Correlation analysis with previously established immunochemical parameters showed that cells with a low cytokeratin-19 (ductal differentiation), high vimentin (mesenchymal marker), and high proliferative phenotype preferentially show higher uptake of mTHPC and subsequent phototoxicity. These results demonstrate high variability of biliary tract cancer cells when subjected to mTHPC-based photodynamic treatment and identify possible markers that could be used in the clinical setting in order to predict the efficiency of PDT and adjust the dose for complete tumour elimination. Neureiter, Daniel verfasserin aut Alinger, Beate verfasserin aut Jansky, Gerhard L. verfasserin aut Berlanda, Juergen verfasserin aut Mkrtchyan, Vahagn verfasserin aut Ocker, Matthias verfasserin aut Plaetzer, Kristjan verfasserin aut Berr, Frieder verfasserin aut Enthalten in Photochemical & photobiological sciences Heidelberg : Springer, 2002 9(2010), 5 vom: 01. Apr., Seite 734-743 (DE-627)342893742 (DE-600)2072584-X 1474-9092 nnns volume:9 year:2010 number:5 day:01 month:04 pages:734-743 https://dx.doi.org/10.1039/b9pp00201d lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2093 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2472 GBV_ILN_2522 GBV_ILN_2812 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4700 GBV_ILN_4753 35.16 ASE 42.14 ASE AR 9 2010 5 01 04 734-743 |
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Kiesslich, Tobias @@aut@@ Neureiter, Daniel @@aut@@ Alinger, Beate @@aut@@ Jansky, Gerhard L. @@aut@@ Berlanda, Juergen @@aut@@ Mkrtchyan, Vahagn @@aut@@ Ocker, Matthias @@aut@@ Plaetzer, Kristjan @@aut@@ Berr, Frieder @@aut@@ |
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Kiesslich, Tobias |
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Kiesslich, Tobias ddc 620 bkl 35.16 bkl 42.14 Uptake and phototoxicity of meso-tetrahydroxyphenyl chlorine are highly variable in human biliary tract cancer cell lines and correlate with markers of differentiation and proliferation |
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620 ASE 35.16 bkl 42.14 bkl Uptake and phototoxicity of meso-tetrahydroxyphenyl chlorine are highly variable in human biliary tract cancer cell lines and correlate with markers of differentiation and proliferation |
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Uptake and phototoxicity of meso-tetrahydroxyphenyl chlorine are highly variable in human biliary tract cancer cell lines and correlate with markers of differentiation and proliferation |
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Uptake and phototoxicity of meso-tetrahydroxyphenyl chlorine are highly variable in human biliary tract cancer cell lines and correlate with markers of differentiation and proliferation |
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Kiesslich, Tobias |
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Photochemical & photobiological sciences |
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Kiesslich, Tobias Neureiter, Daniel Alinger, Beate Jansky, Gerhard L. Berlanda, Juergen Mkrtchyan, Vahagn Ocker, Matthias Plaetzer, Kristjan Berr, Frieder |
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verfasserin |
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uptake and phototoxicity of meso-tetrahydroxyphenyl chlorine are highly variable in human biliary tract cancer cell lines and correlate with markers of differentiation and proliferation |
| title_auth |
Uptake and phototoxicity of meso-tetrahydroxyphenyl chlorine are highly variable in human biliary tract cancer cell lines and correlate with markers of differentiation and proliferation |
| abstract |
Abstract Photodynamic therapy (PDT) using Photofrin® and, recently, Foscan® has gained broad acceptance for palliative treatment of non-resectable cholangiocarcinoma (CC). No information, however, is available whether the phenotype of CC tumour cells has an effect on the efficiency of the treatment. Using a well-characterised set of n = 9 biliary tract cancer cell lines this study investigated the uptake, phototoxicity, and release of meso-tetrahydroxyphenyl chlorine (mTHPC, Foscan®) after incubation with 200 or 400 ng $ ml^{−1} $ mTHPC. For uptake of mTHPC we found great variations between the individual cell lines (up to a factor 2), resulting in even more pronounced differences in phototoxicity. Based on statistical classification by hierarchical cluster analysis, two groups of cell lines can be distinguished which are characterised by either high or low susceptibility towards mTHPC-based photodynamic treatment. Correlation analysis with previously established immunochemical parameters showed that cells with a low cytokeratin-19 (ductal differentiation), high vimentin (mesenchymal marker), and high proliferative phenotype preferentially show higher uptake of mTHPC and subsequent phototoxicity. These results demonstrate high variability of biliary tract cancer cells when subjected to mTHPC-based photodynamic treatment and identify possible markers that could be used in the clinical setting in order to predict the efficiency of PDT and adjust the dose for complete tumour elimination. © The Royal Society of Chemistry and Owner Societies 2010 |
| abstractGer |
Abstract Photodynamic therapy (PDT) using Photofrin® and, recently, Foscan® has gained broad acceptance for palliative treatment of non-resectable cholangiocarcinoma (CC). No information, however, is available whether the phenotype of CC tumour cells has an effect on the efficiency of the treatment. Using a well-characterised set of n = 9 biliary tract cancer cell lines this study investigated the uptake, phototoxicity, and release of meso-tetrahydroxyphenyl chlorine (mTHPC, Foscan®) after incubation with 200 or 400 ng $ ml^{−1} $ mTHPC. For uptake of mTHPC we found great variations between the individual cell lines (up to a factor 2), resulting in even more pronounced differences in phototoxicity. Based on statistical classification by hierarchical cluster analysis, two groups of cell lines can be distinguished which are characterised by either high or low susceptibility towards mTHPC-based photodynamic treatment. Correlation analysis with previously established immunochemical parameters showed that cells with a low cytokeratin-19 (ductal differentiation), high vimentin (mesenchymal marker), and high proliferative phenotype preferentially show higher uptake of mTHPC and subsequent phototoxicity. These results demonstrate high variability of biliary tract cancer cells when subjected to mTHPC-based photodynamic treatment and identify possible markers that could be used in the clinical setting in order to predict the efficiency of PDT and adjust the dose for complete tumour elimination. © The Royal Society of Chemistry and Owner Societies 2010 |
| abstract_unstemmed |
Abstract Photodynamic therapy (PDT) using Photofrin® and, recently, Foscan® has gained broad acceptance for palliative treatment of non-resectable cholangiocarcinoma (CC). No information, however, is available whether the phenotype of CC tumour cells has an effect on the efficiency of the treatment. Using a well-characterised set of n = 9 biliary tract cancer cell lines this study investigated the uptake, phototoxicity, and release of meso-tetrahydroxyphenyl chlorine (mTHPC, Foscan®) after incubation with 200 or 400 ng $ ml^{−1} $ mTHPC. For uptake of mTHPC we found great variations between the individual cell lines (up to a factor 2), resulting in even more pronounced differences in phototoxicity. Based on statistical classification by hierarchical cluster analysis, two groups of cell lines can be distinguished which are characterised by either high or low susceptibility towards mTHPC-based photodynamic treatment. Correlation analysis with previously established immunochemical parameters showed that cells with a low cytokeratin-19 (ductal differentiation), high vimentin (mesenchymal marker), and high proliferative phenotype preferentially show higher uptake of mTHPC and subsequent phototoxicity. These results demonstrate high variability of biliary tract cancer cells when subjected to mTHPC-based photodynamic treatment and identify possible markers that could be used in the clinical setting in order to predict the efficiency of PDT and adjust the dose for complete tumour elimination. © The Royal Society of Chemistry and Owner Societies 2010 |
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Uptake and phototoxicity of meso-tetrahydroxyphenyl chlorine are highly variable in human biliary tract cancer cell lines and correlate with markers of differentiation and proliferation |
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Neureiter, Daniel Alinger, Beate Jansky, Gerhard L. Berlanda, Juergen Mkrtchyan, Vahagn Ocker, Matthias Plaetzer, Kristjan Berr, Frieder |
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Neureiter, Daniel Alinger, Beate Jansky, Gerhard L. Berlanda, Juergen Mkrtchyan, Vahagn Ocker, Matthias Plaetzer, Kristjan Berr, Frieder |
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