Biopesticide production using Bacillus thuringiensis kurstaki by valorization of starch industry wastewater and effluent from aerobic, anaerobic digestion
Introduction Unconventional alternatives such as aerobic and anaerobic effluent from starch industry contain essential nutrients for Btk active ingredient synthesis. Effluent from starch industry is rich in carbon and nitrogen and can replace expensive feedstock used during the fermentation process....
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Ndao, Adama [verfasserIn] Sellamuthu, B. [verfasserIn] Kumar, Lalit R. [verfasserIn] Tyagi, R. D. [verfasserIn] Valéro, J. R. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2021 |
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| Schlagwörter: |
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| Anmerkung: |
© Jiangnan University 2021 |
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| Übergeordnetes Werk: |
Enthalten in: Systems microbiology and biomanufacturing - Cham : Springer Nature Switzerland, 2021, 1(2021), 4 vom: 16. Juli, Seite 494-504 |
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| Übergeordnetes Werk: |
volume:1 ; year:2021 ; number:4 ; day:16 ; month:07 ; pages:494-504 |
| Links: |
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| DOI / URN: |
10.1007/s43393-021-00043-x |
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| Katalog-ID: |
SPR045069425 |
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| 520 | |a Introduction Unconventional alternatives such as aerobic and anaerobic effluent from starch industry contain essential nutrients for Btk active ingredient synthesis. Effluent from starch industry is rich in carbon and nitrogen and can replace expensive feedstock used during the fermentation process. Objectives The main objective of this study was to achieve a biopesticide formulation from starch industry wastewater (SIW) with high entomotoxicity (UI/ml) of larvae comparable to Foray 76B, which is a commercial biopesticide. Methods Bacillus thuringiensis var kurstaki HD1 (Btk) strain was cultivated and sub-cultured to aerobic, anaerobic digested effluent and SIW. Pre-treatment was carried on these different substrates to enhance the residual carbon required for Btk growth and delta endotoxin synthesis. After 48 hours of fermentation, cells count and delta-endotoxin were determined. A biopesticide formulation containing fermented broth and adjuvants was fed to larvae to determine larvae mortality. Results Btk cell growth and sporulation profile in SIW media displayed a high total cell count and viable spores compared to btk growth in anaerobic or aerobic media after 48h fermentation. The maximum endotoxin concentration in the SIW medium was 435μg/mL, whereas, in anaerobic and aerobic effluent, the maximum concentrations were at 161 μg/mL and 136 μg/mL, respectively. When acidic treatment was performed at pH 2 for these substrates, entomotoxicity obtained from aerobic and anaerobic biopesticide formulations displayed significantly higher entomoxicity than the untreated ones. The entomotoxicity of SIW treated at pH 2 was equivalent to the standard Foray 76B which is 20,000 IU/μL. Conclusion Anaerobic and aerobic effluent did not contain enough total organic carbon to augment Btk growth and entomotoxicity. Substrates pre-treated at pH 2 provided significant organic matter for Btk growth and resulted in larval mortality equivalent to the com ercial biopesticide Foray 76B. | ||
| 650 | 4 | |a Aerobic digested effluent |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Anaerobic digested effluent |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Effluent valorization |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Biopesticide |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Sellamuthu, B. |e verfasserin |4 aut | |
| 700 | 1 | |a Kumar, Lalit R. |e verfasserin |4 aut | |
| 700 | 1 | |a Tyagi, R. D. |e verfasserin |4 aut | |
| 700 | 1 | |a Valéro, J. R. |e verfasserin |4 aut | |
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10.1007/s43393-021-00043-x doi (DE-627)SPR045069425 (SPR)s43393-021-00043-x-e DE-627 ger DE-627 rakwb eng 570 ASE Ndao, Adama verfasserin aut Biopesticide production using Bacillus thuringiensis kurstaki by valorization of starch industry wastewater and effluent from aerobic, anaerobic digestion 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jiangnan University 2021 Introduction Unconventional alternatives such as aerobic and anaerobic effluent from starch industry contain essential nutrients for Btk active ingredient synthesis. Effluent from starch industry is rich in carbon and nitrogen and can replace expensive feedstock used during the fermentation process. Objectives The main objective of this study was to achieve a biopesticide formulation from starch industry wastewater (SIW) with high entomotoxicity (UI/ml) of larvae comparable to Foray 76B, which is a commercial biopesticide. Methods Bacillus thuringiensis var kurstaki HD1 (Btk) strain was cultivated and sub-cultured to aerobic, anaerobic digested effluent and SIW. Pre-treatment was carried on these different substrates to enhance the residual carbon required for Btk growth and delta endotoxin synthesis. After 48 hours of fermentation, cells count and delta-endotoxin were determined. A biopesticide formulation containing fermented broth and adjuvants was fed to larvae to determine larvae mortality. Results Btk cell growth and sporulation profile in SIW media displayed a high total cell count and viable spores compared to btk growth in anaerobic or aerobic media after 48h fermentation. The maximum endotoxin concentration in the SIW medium was 435μg/mL, whereas, in anaerobic and aerobic effluent, the maximum concentrations were at 161 μg/mL and 136 μg/mL, respectively. When acidic treatment was performed at pH 2 for these substrates, entomotoxicity obtained from aerobic and anaerobic biopesticide formulations displayed significantly higher entomoxicity than the untreated ones. The entomotoxicity of SIW treated at pH 2 was equivalent to the standard Foray 76B which is 20,000 IU/μL. Conclusion Anaerobic and aerobic effluent did not contain enough total organic carbon to augment Btk growth and entomotoxicity. Substrates pre-treated at pH 2 provided significant organic matter for Btk growth and resulted in larval mortality equivalent to the com ercial biopesticide Foray 76B. Aerobic digested effluent (dpeaa)DE-He213 Anaerobic digested effluent (dpeaa)DE-He213 Effluent valorization (dpeaa)DE-He213 Biopesticide (dpeaa)DE-He213 Sellamuthu, B. verfasserin aut Kumar, Lalit R. verfasserin aut Tyagi, R. D. verfasserin aut Valéro, J. R. verfasserin aut Enthalten in Systems microbiology and biomanufacturing Cham : Springer Nature Switzerland, 2021 1(2021), 4 vom: 16. Juli, Seite 494-504 (DE-627)1726726673 (DE-600)3033857-8 2662-7663 nnns volume:1 year:2021 number:4 day:16 month:07 pages:494-504 https://dx.doi.org/10.1007/s43393-021-00043-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 1 2021 4 16 07 494-504 |
| spelling |
10.1007/s43393-021-00043-x doi (DE-627)SPR045069425 (SPR)s43393-021-00043-x-e DE-627 ger DE-627 rakwb eng 570 ASE Ndao, Adama verfasserin aut Biopesticide production using Bacillus thuringiensis kurstaki by valorization of starch industry wastewater and effluent from aerobic, anaerobic digestion 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jiangnan University 2021 Introduction Unconventional alternatives such as aerobic and anaerobic effluent from starch industry contain essential nutrients for Btk active ingredient synthesis. Effluent from starch industry is rich in carbon and nitrogen and can replace expensive feedstock used during the fermentation process. Objectives The main objective of this study was to achieve a biopesticide formulation from starch industry wastewater (SIW) with high entomotoxicity (UI/ml) of larvae comparable to Foray 76B, which is a commercial biopesticide. Methods Bacillus thuringiensis var kurstaki HD1 (Btk) strain was cultivated and sub-cultured to aerobic, anaerobic digested effluent and SIW. Pre-treatment was carried on these different substrates to enhance the residual carbon required for Btk growth and delta endotoxin synthesis. After 48 hours of fermentation, cells count and delta-endotoxin were determined. A biopesticide formulation containing fermented broth and adjuvants was fed to larvae to determine larvae mortality. Results Btk cell growth and sporulation profile in SIW media displayed a high total cell count and viable spores compared to btk growth in anaerobic or aerobic media after 48h fermentation. The maximum endotoxin concentration in the SIW medium was 435μg/mL, whereas, in anaerobic and aerobic effluent, the maximum concentrations were at 161 μg/mL and 136 μg/mL, respectively. When acidic treatment was performed at pH 2 for these substrates, entomotoxicity obtained from aerobic and anaerobic biopesticide formulations displayed significantly higher entomoxicity than the untreated ones. The entomotoxicity of SIW treated at pH 2 was equivalent to the standard Foray 76B which is 20,000 IU/μL. Conclusion Anaerobic and aerobic effluent did not contain enough total organic carbon to augment Btk growth and entomotoxicity. Substrates pre-treated at pH 2 provided significant organic matter for Btk growth and resulted in larval mortality equivalent to the com ercial biopesticide Foray 76B. Aerobic digested effluent (dpeaa)DE-He213 Anaerobic digested effluent (dpeaa)DE-He213 Effluent valorization (dpeaa)DE-He213 Biopesticide (dpeaa)DE-He213 Sellamuthu, B. verfasserin aut Kumar, Lalit R. verfasserin aut Tyagi, R. D. verfasserin aut Valéro, J. R. verfasserin aut Enthalten in Systems microbiology and biomanufacturing Cham : Springer Nature Switzerland, 2021 1(2021), 4 vom: 16. Juli, Seite 494-504 (DE-627)1726726673 (DE-600)3033857-8 2662-7663 nnns volume:1 year:2021 number:4 day:16 month:07 pages:494-504 https://dx.doi.org/10.1007/s43393-021-00043-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 1 2021 4 16 07 494-504 |
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10.1007/s43393-021-00043-x doi (DE-627)SPR045069425 (SPR)s43393-021-00043-x-e DE-627 ger DE-627 rakwb eng 570 ASE Ndao, Adama verfasserin aut Biopesticide production using Bacillus thuringiensis kurstaki by valorization of starch industry wastewater and effluent from aerobic, anaerobic digestion 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jiangnan University 2021 Introduction Unconventional alternatives such as aerobic and anaerobic effluent from starch industry contain essential nutrients for Btk active ingredient synthesis. Effluent from starch industry is rich in carbon and nitrogen and can replace expensive feedstock used during the fermentation process. Objectives The main objective of this study was to achieve a biopesticide formulation from starch industry wastewater (SIW) with high entomotoxicity (UI/ml) of larvae comparable to Foray 76B, which is a commercial biopesticide. Methods Bacillus thuringiensis var kurstaki HD1 (Btk) strain was cultivated and sub-cultured to aerobic, anaerobic digested effluent and SIW. Pre-treatment was carried on these different substrates to enhance the residual carbon required for Btk growth and delta endotoxin synthesis. After 48 hours of fermentation, cells count and delta-endotoxin were determined. A biopesticide formulation containing fermented broth and adjuvants was fed to larvae to determine larvae mortality. Results Btk cell growth and sporulation profile in SIW media displayed a high total cell count and viable spores compared to btk growth in anaerobic or aerobic media after 48h fermentation. The maximum endotoxin concentration in the SIW medium was 435μg/mL, whereas, in anaerobic and aerobic effluent, the maximum concentrations were at 161 μg/mL and 136 μg/mL, respectively. When acidic treatment was performed at pH 2 for these substrates, entomotoxicity obtained from aerobic and anaerobic biopesticide formulations displayed significantly higher entomoxicity than the untreated ones. The entomotoxicity of SIW treated at pH 2 was equivalent to the standard Foray 76B which is 20,000 IU/μL. Conclusion Anaerobic and aerobic effluent did not contain enough total organic carbon to augment Btk growth and entomotoxicity. Substrates pre-treated at pH 2 provided significant organic matter for Btk growth and resulted in larval mortality equivalent to the com ercial biopesticide Foray 76B. Aerobic digested effluent (dpeaa)DE-He213 Anaerobic digested effluent (dpeaa)DE-He213 Effluent valorization (dpeaa)DE-He213 Biopesticide (dpeaa)DE-He213 Sellamuthu, B. verfasserin aut Kumar, Lalit R. verfasserin aut Tyagi, R. D. verfasserin aut Valéro, J. R. verfasserin aut Enthalten in Systems microbiology and biomanufacturing Cham : Springer Nature Switzerland, 2021 1(2021), 4 vom: 16. Juli, Seite 494-504 (DE-627)1726726673 (DE-600)3033857-8 2662-7663 nnns volume:1 year:2021 number:4 day:16 month:07 pages:494-504 https://dx.doi.org/10.1007/s43393-021-00043-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 1 2021 4 16 07 494-504 |
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10.1007/s43393-021-00043-x doi (DE-627)SPR045069425 (SPR)s43393-021-00043-x-e DE-627 ger DE-627 rakwb eng 570 ASE Ndao, Adama verfasserin aut Biopesticide production using Bacillus thuringiensis kurstaki by valorization of starch industry wastewater and effluent from aerobic, anaerobic digestion 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jiangnan University 2021 Introduction Unconventional alternatives such as aerobic and anaerobic effluent from starch industry contain essential nutrients for Btk active ingredient synthesis. Effluent from starch industry is rich in carbon and nitrogen and can replace expensive feedstock used during the fermentation process. Objectives The main objective of this study was to achieve a biopesticide formulation from starch industry wastewater (SIW) with high entomotoxicity (UI/ml) of larvae comparable to Foray 76B, which is a commercial biopesticide. Methods Bacillus thuringiensis var kurstaki HD1 (Btk) strain was cultivated and sub-cultured to aerobic, anaerobic digested effluent and SIW. Pre-treatment was carried on these different substrates to enhance the residual carbon required for Btk growth and delta endotoxin synthesis. After 48 hours of fermentation, cells count and delta-endotoxin were determined. A biopesticide formulation containing fermented broth and adjuvants was fed to larvae to determine larvae mortality. Results Btk cell growth and sporulation profile in SIW media displayed a high total cell count and viable spores compared to btk growth in anaerobic or aerobic media after 48h fermentation. The maximum endotoxin concentration in the SIW medium was 435μg/mL, whereas, in anaerobic and aerobic effluent, the maximum concentrations were at 161 μg/mL and 136 μg/mL, respectively. When acidic treatment was performed at pH 2 for these substrates, entomotoxicity obtained from aerobic and anaerobic biopesticide formulations displayed significantly higher entomoxicity than the untreated ones. The entomotoxicity of SIW treated at pH 2 was equivalent to the standard Foray 76B which is 20,000 IU/μL. Conclusion Anaerobic and aerobic effluent did not contain enough total organic carbon to augment Btk growth and entomotoxicity. Substrates pre-treated at pH 2 provided significant organic matter for Btk growth and resulted in larval mortality equivalent to the com ercial biopesticide Foray 76B. Aerobic digested effluent (dpeaa)DE-He213 Anaerobic digested effluent (dpeaa)DE-He213 Effluent valorization (dpeaa)DE-He213 Biopesticide (dpeaa)DE-He213 Sellamuthu, B. verfasserin aut Kumar, Lalit R. verfasserin aut Tyagi, R. D. verfasserin aut Valéro, J. R. verfasserin aut Enthalten in Systems microbiology and biomanufacturing Cham : Springer Nature Switzerland, 2021 1(2021), 4 vom: 16. Juli, Seite 494-504 (DE-627)1726726673 (DE-600)3033857-8 2662-7663 nnns volume:1 year:2021 number:4 day:16 month:07 pages:494-504 https://dx.doi.org/10.1007/s43393-021-00043-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 1 2021 4 16 07 494-504 |
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10.1007/s43393-021-00043-x doi (DE-627)SPR045069425 (SPR)s43393-021-00043-x-e DE-627 ger DE-627 rakwb eng 570 ASE Ndao, Adama verfasserin aut Biopesticide production using Bacillus thuringiensis kurstaki by valorization of starch industry wastewater and effluent from aerobic, anaerobic digestion 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Jiangnan University 2021 Introduction Unconventional alternatives such as aerobic and anaerobic effluent from starch industry contain essential nutrients for Btk active ingredient synthesis. Effluent from starch industry is rich in carbon and nitrogen and can replace expensive feedstock used during the fermentation process. Objectives The main objective of this study was to achieve a biopesticide formulation from starch industry wastewater (SIW) with high entomotoxicity (UI/ml) of larvae comparable to Foray 76B, which is a commercial biopesticide. Methods Bacillus thuringiensis var kurstaki HD1 (Btk) strain was cultivated and sub-cultured to aerobic, anaerobic digested effluent and SIW. Pre-treatment was carried on these different substrates to enhance the residual carbon required for Btk growth and delta endotoxin synthesis. After 48 hours of fermentation, cells count and delta-endotoxin were determined. A biopesticide formulation containing fermented broth and adjuvants was fed to larvae to determine larvae mortality. Results Btk cell growth and sporulation profile in SIW media displayed a high total cell count and viable spores compared to btk growth in anaerobic or aerobic media after 48h fermentation. The maximum endotoxin concentration in the SIW medium was 435μg/mL, whereas, in anaerobic and aerobic effluent, the maximum concentrations were at 161 μg/mL and 136 μg/mL, respectively. When acidic treatment was performed at pH 2 for these substrates, entomotoxicity obtained from aerobic and anaerobic biopesticide formulations displayed significantly higher entomoxicity than the untreated ones. The entomotoxicity of SIW treated at pH 2 was equivalent to the standard Foray 76B which is 20,000 IU/μL. Conclusion Anaerobic and aerobic effluent did not contain enough total organic carbon to augment Btk growth and entomotoxicity. Substrates pre-treated at pH 2 provided significant organic matter for Btk growth and resulted in larval mortality equivalent to the com ercial biopesticide Foray 76B. Aerobic digested effluent (dpeaa)DE-He213 Anaerobic digested effluent (dpeaa)DE-He213 Effluent valorization (dpeaa)DE-He213 Biopesticide (dpeaa)DE-He213 Sellamuthu, B. verfasserin aut Kumar, Lalit R. verfasserin aut Tyagi, R. D. verfasserin aut Valéro, J. R. verfasserin aut Enthalten in Systems microbiology and biomanufacturing Cham : Springer Nature Switzerland, 2021 1(2021), 4 vom: 16. Juli, Seite 494-504 (DE-627)1726726673 (DE-600)3033857-8 2662-7663 nnns volume:1 year:2021 number:4 day:16 month:07 pages:494-504 https://dx.doi.org/10.1007/s43393-021-00043-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 1 2021 4 16 07 494-504 |
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Enthalten in Systems microbiology and biomanufacturing 1(2021), 4 vom: 16. Juli, Seite 494-504 volume:1 year:2021 number:4 day:16 month:07 pages:494-504 |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR045069425</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230520000558.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">210914s2021 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s43393-021-00043-x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR045069425</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s43393-021-00043-x-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Ndao, Adama</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Biopesticide production using Bacillus thuringiensis kurstaki by valorization of starch industry wastewater and effluent from aerobic, anaerobic digestion</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2021</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Jiangnan University 2021</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Introduction Unconventional alternatives such as aerobic and anaerobic effluent from starch industry contain essential nutrients for Btk active ingredient synthesis. Effluent from starch industry is rich in carbon and nitrogen and can replace expensive feedstock used during the fermentation process. Objectives The main objective of this study was to achieve a biopesticide formulation from starch industry wastewater (SIW) with high entomotoxicity (UI/ml) of larvae comparable to Foray 76B, which is a commercial biopesticide. Methods Bacillus thuringiensis var kurstaki HD1 (Btk) strain was cultivated and sub-cultured to aerobic, anaerobic digested effluent and SIW. Pre-treatment was carried on these different substrates to enhance the residual carbon required for Btk growth and delta endotoxin synthesis. After 48 hours of fermentation, cells count and delta-endotoxin were determined. A biopesticide formulation containing fermented broth and adjuvants was fed to larvae to determine larvae mortality. Results Btk cell growth and sporulation profile in SIW media displayed a high total cell count and viable spores compared to btk growth in anaerobic or aerobic media after 48h fermentation. The maximum endotoxin concentration in the SIW medium was 435μg/mL, whereas, in anaerobic and aerobic effluent, the maximum concentrations were at 161 μg/mL and 136 μg/mL, respectively. When acidic treatment was performed at pH 2 for these substrates, entomotoxicity obtained from aerobic and anaerobic biopesticide formulations displayed significantly higher entomoxicity than the untreated ones. The entomotoxicity of SIW treated at pH 2 was equivalent to the standard Foray 76B which is 20,000 IU/μL. Conclusion Anaerobic and aerobic effluent did not contain enough total organic carbon to augment Btk growth and entomotoxicity. 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Biopesticide production using Bacillus thuringiensis kurstaki by valorization of starch industry wastewater and effluent from aerobic, anaerobic digestion |
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biopesticide production using bacillus thuringiensis kurstaki by valorization of starch industry wastewater and effluent from aerobic, anaerobic digestion |
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Biopesticide production using Bacillus thuringiensis kurstaki by valorization of starch industry wastewater and effluent from aerobic, anaerobic digestion |
| abstract |
Introduction Unconventional alternatives such as aerobic and anaerobic effluent from starch industry contain essential nutrients for Btk active ingredient synthesis. Effluent from starch industry is rich in carbon and nitrogen and can replace expensive feedstock used during the fermentation process. Objectives The main objective of this study was to achieve a biopesticide formulation from starch industry wastewater (SIW) with high entomotoxicity (UI/ml) of larvae comparable to Foray 76B, which is a commercial biopesticide. Methods Bacillus thuringiensis var kurstaki HD1 (Btk) strain was cultivated and sub-cultured to aerobic, anaerobic digested effluent and SIW. Pre-treatment was carried on these different substrates to enhance the residual carbon required for Btk growth and delta endotoxin synthesis. After 48 hours of fermentation, cells count and delta-endotoxin were determined. A biopesticide formulation containing fermented broth and adjuvants was fed to larvae to determine larvae mortality. Results Btk cell growth and sporulation profile in SIW media displayed a high total cell count and viable spores compared to btk growth in anaerobic or aerobic media after 48h fermentation. The maximum endotoxin concentration in the SIW medium was 435μg/mL, whereas, in anaerobic and aerobic effluent, the maximum concentrations were at 161 μg/mL and 136 μg/mL, respectively. When acidic treatment was performed at pH 2 for these substrates, entomotoxicity obtained from aerobic and anaerobic biopesticide formulations displayed significantly higher entomoxicity than the untreated ones. The entomotoxicity of SIW treated at pH 2 was equivalent to the standard Foray 76B which is 20,000 IU/μL. Conclusion Anaerobic and aerobic effluent did not contain enough total organic carbon to augment Btk growth and entomotoxicity. Substrates pre-treated at pH 2 provided significant organic matter for Btk growth and resulted in larval mortality equivalent to the com ercial biopesticide Foray 76B. © Jiangnan University 2021 |
| abstractGer |
Introduction Unconventional alternatives such as aerobic and anaerobic effluent from starch industry contain essential nutrients for Btk active ingredient synthesis. Effluent from starch industry is rich in carbon and nitrogen and can replace expensive feedstock used during the fermentation process. Objectives The main objective of this study was to achieve a biopesticide formulation from starch industry wastewater (SIW) with high entomotoxicity (UI/ml) of larvae comparable to Foray 76B, which is a commercial biopesticide. Methods Bacillus thuringiensis var kurstaki HD1 (Btk) strain was cultivated and sub-cultured to aerobic, anaerobic digested effluent and SIW. Pre-treatment was carried on these different substrates to enhance the residual carbon required for Btk growth and delta endotoxin synthesis. After 48 hours of fermentation, cells count and delta-endotoxin were determined. A biopesticide formulation containing fermented broth and adjuvants was fed to larvae to determine larvae mortality. Results Btk cell growth and sporulation profile in SIW media displayed a high total cell count and viable spores compared to btk growth in anaerobic or aerobic media after 48h fermentation. The maximum endotoxin concentration in the SIW medium was 435μg/mL, whereas, in anaerobic and aerobic effluent, the maximum concentrations were at 161 μg/mL and 136 μg/mL, respectively. When acidic treatment was performed at pH 2 for these substrates, entomotoxicity obtained from aerobic and anaerobic biopesticide formulations displayed significantly higher entomoxicity than the untreated ones. The entomotoxicity of SIW treated at pH 2 was equivalent to the standard Foray 76B which is 20,000 IU/μL. Conclusion Anaerobic and aerobic effluent did not contain enough total organic carbon to augment Btk growth and entomotoxicity. Substrates pre-treated at pH 2 provided significant organic matter for Btk growth and resulted in larval mortality equivalent to the com ercial biopesticide Foray 76B. © Jiangnan University 2021 |
| abstract_unstemmed |
Introduction Unconventional alternatives such as aerobic and anaerobic effluent from starch industry contain essential nutrients for Btk active ingredient synthesis. Effluent from starch industry is rich in carbon and nitrogen and can replace expensive feedstock used during the fermentation process. Objectives The main objective of this study was to achieve a biopesticide formulation from starch industry wastewater (SIW) with high entomotoxicity (UI/ml) of larvae comparable to Foray 76B, which is a commercial biopesticide. Methods Bacillus thuringiensis var kurstaki HD1 (Btk) strain was cultivated and sub-cultured to aerobic, anaerobic digested effluent and SIW. Pre-treatment was carried on these different substrates to enhance the residual carbon required for Btk growth and delta endotoxin synthesis. After 48 hours of fermentation, cells count and delta-endotoxin were determined. A biopesticide formulation containing fermented broth and adjuvants was fed to larvae to determine larvae mortality. Results Btk cell growth and sporulation profile in SIW media displayed a high total cell count and viable spores compared to btk growth in anaerobic or aerobic media after 48h fermentation. The maximum endotoxin concentration in the SIW medium was 435μg/mL, whereas, in anaerobic and aerobic effluent, the maximum concentrations were at 161 μg/mL and 136 μg/mL, respectively. When acidic treatment was performed at pH 2 for these substrates, entomotoxicity obtained from aerobic and anaerobic biopesticide formulations displayed significantly higher entomoxicity than the untreated ones. The entomotoxicity of SIW treated at pH 2 was equivalent to the standard Foray 76B which is 20,000 IU/μL. Conclusion Anaerobic and aerobic effluent did not contain enough total organic carbon to augment Btk growth and entomotoxicity. Substrates pre-treated at pH 2 provided significant organic matter for Btk growth and resulted in larval mortality equivalent to the com ercial biopesticide Foray 76B. © Jiangnan University 2021 |
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| title_short |
Biopesticide production using Bacillus thuringiensis kurstaki by valorization of starch industry wastewater and effluent from aerobic, anaerobic digestion |
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https://dx.doi.org/10.1007/s43393-021-00043-x |
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Sellamuthu, B. Kumar, Lalit R. Tyagi, R. D. Valéro, J. R. |
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Sellamuthu, B. Kumar, Lalit R. Tyagi, R. D. Valéro, J. R. |
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| doi_str |
10.1007/s43393-021-00043-x |
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2024-07-03T13:35:00.007Z |
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|
| score |
7.40007 |