Genome-Wide Identification and Expression Analysis of the Barrel Medic (Medicago truncatula) and Alfalfa (Medicago sativa L.) Basic Helix-Loop-Helix Transcription Factor Family Under Salt and Drought Stresses
Abstract The basic helix loop helix (bHLH) transcription factor comprises one of the largest plant-specific transcriptional regulators in plant growth and development that response to biotic and abiotic stresses. Many members of bHLH play essential roles in the growth of root hair and response to dr...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Ndayambaza, Boniface [verfasserIn] Jin, Xiaoyu [verfasserIn] Min, Xueyang [verfasserIn] Lin, Xiaoshan [verfasserIn] Yin, Xiaofan [verfasserIn] Liu, Wenxian [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer Science+Business Media, LLC, part of Springer Nature 2020 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of plant growth regulation - New York, NY : Springer, 1982, 40(2020), 5 vom: 30. Okt., Seite 2058-2078 |
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| Übergeordnetes Werk: |
volume:40 ; year:2020 ; number:5 ; day:30 ; month:10 ; pages:2058-2078 |
| Links: |
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| DOI / URN: |
10.1007/s00344-020-10252-8 |
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| Katalog-ID: |
SPR04521199X |
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| 245 | 1 | 0 | |a Genome-Wide Identification and Expression Analysis of the Barrel Medic (Medicago truncatula) and Alfalfa (Medicago sativa L.) Basic Helix-Loop-Helix Transcription Factor Family Under Salt and Drought Stresses |
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| 520 | |a Abstract The basic helix loop helix (bHLH) transcription factor comprises one of the largest plant-specific transcriptional regulators in plant growth and development that response to biotic and abiotic stresses. Many members of bHLH play essential roles in the growth of root hair and response to drought, salt, and cold stresses. The family of bHLH genes has been found in many species; nevertheless, the barrel medic and alfalfa species still have a minute gap of bHLH new members thus far. This research aims to identify members of the bHLH family in barrel medic and alfalfa and elucidate their expression pattern level, network analysis, predictive 3D modeling and phylogenetic relationships. Here, we identified and characterized the bHLH gene family in both barrel medic and alfalfa plants and their genes expression response to drought, salinity, and cold stresses. A total of 159 MtbHLH and 133 MsbHLH genes were identified and characterized, divided into 18 subgroups and 17 subgroups, respectively. As a ubiquitous and popular method, neighbor-joining clustering was used. Based on the phylogenetic analyses, the VIII and IX subfamily and X subfamily were selected as the stress-related subfamily in these two species. The 154 MtbHLH genes were progressively distributed on the 8 chromosomes and 23 tandem duplicated genes, and 44 duplicated genes segments were detected in MtbHLH family. The analyses of gene ontology discovered the bHLH predominantly functions in protein and DNA binding in these two species. The results of Ka/Ks were < 1, which showed that the most orthologous of the bHLH gene values was found between A. thaliana and M. truncatula species. Remarkably, 7 MtbHLH and 10 MsbHLH genes were selected and validated with qRT-PCR after the treatment’s samples sampled under stressed abiotic conditions. The similar expression patterns between M. truncatula and M. sativa L have demonstrated identical expression patterns level in the root, and contrasting patterns in the stems and leaves were diverse. It was highlighted that the gene expression analyses of 17 bHLH genes were up-regulated to stresses, respectively, apart from some genes that were timely trended down-regulated to control (0 h). This study provided a concise understanding of the tissue specific of bHLH gene functions in genome-wide levels under drought, salt, and cold stresses. Our analyses provide the first insights onto the M. truncatula and M. sativa L evolution that contributes to molecular breeding for improving plant yield and stress tolerance. | ||
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| 650 | 4 | |a Abiotic stress |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Gene expression analysis |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Jin, Xiaoyu |e verfasserin |4 aut | |
| 700 | 1 | |a Min, Xueyang |e verfasserin |4 aut | |
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| 700 | 1 | |a Yin, Xiaofan |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Wenxian |e verfasserin |4 aut | |
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10.1007/s00344-020-10252-8 doi (DE-627)SPR04521199X (SPR)s00344-020-10252-8-e DE-627 ger DE-627 rakwb eng 580 ASE 580 ASE 42.41 bkl 42.42 bkl Ndayambaza, Boniface verfasserin aut Genome-Wide Identification and Expression Analysis of the Barrel Medic (Medicago truncatula) and Alfalfa (Medicago sativa L.) Basic Helix-Loop-Helix Transcription Factor Family Under Salt and Drought Stresses 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2020 Abstract The basic helix loop helix (bHLH) transcription factor comprises one of the largest plant-specific transcriptional regulators in plant growth and development that response to biotic and abiotic stresses. Many members of bHLH play essential roles in the growth of root hair and response to drought, salt, and cold stresses. The family of bHLH genes has been found in many species; nevertheless, the barrel medic and alfalfa species still have a minute gap of bHLH new members thus far. This research aims to identify members of the bHLH family in barrel medic and alfalfa and elucidate their expression pattern level, network analysis, predictive 3D modeling and phylogenetic relationships. Here, we identified and characterized the bHLH gene family in both barrel medic and alfalfa plants and their genes expression response to drought, salinity, and cold stresses. A total of 159 MtbHLH and 133 MsbHLH genes were identified and characterized, divided into 18 subgroups and 17 subgroups, respectively. As a ubiquitous and popular method, neighbor-joining clustering was used. Based on the phylogenetic analyses, the VIII and IX subfamily and X subfamily were selected as the stress-related subfamily in these two species. The 154 MtbHLH genes were progressively distributed on the 8 chromosomes and 23 tandem duplicated genes, and 44 duplicated genes segments were detected in MtbHLH family. The analyses of gene ontology discovered the bHLH predominantly functions in protein and DNA binding in these two species. The results of Ka/Ks were < 1, which showed that the most orthologous of the bHLH gene values was found between A. thaliana and M. truncatula species. Remarkably, 7 MtbHLH and 10 MsbHLH genes were selected and validated with qRT-PCR after the treatment’s samples sampled under stressed abiotic conditions. The similar expression patterns between M. truncatula and M. sativa L have demonstrated identical expression patterns level in the root, and contrasting patterns in the stems and leaves were diverse. It was highlighted that the gene expression analyses of 17 bHLH genes were up-regulated to stresses, respectively, apart from some genes that were timely trended down-regulated to control (0 h). This study provided a concise understanding of the tissue specific of bHLH gene functions in genome-wide levels under drought, salt, and cold stresses. Our analyses provide the first insights onto the M. truncatula and M. sativa L evolution that contributes to molecular breeding for improving plant yield and stress tolerance. Barrel medic and alfalfa (dpeaa)DE-He213 transcription factors (dpeaa)DE-He213 Phylogenetic analysis (dpeaa)DE-He213 Abiotic stress (dpeaa)DE-He213 Gene expression analysis (dpeaa)DE-He213 Jin, Xiaoyu verfasserin aut Min, Xueyang verfasserin aut Lin, Xiaoshan verfasserin aut Yin, Xiaofan verfasserin aut Liu, Wenxian verfasserin aut Enthalten in Journal of plant growth regulation New York, NY : Springer, 1982 40(2020), 5 vom: 30. Okt., Seite 2058-2078 (DE-627)254630448 (DE-600)1462091-1 1435-8107 nnns volume:40 year:2020 number:5 day:30 month:10 pages:2058-2078 https://dx.doi.org/10.1007/s00344-020-10252-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.41 ASE 42.42 ASE AR 40 2020 5 30 10 2058-2078 |
| spelling |
10.1007/s00344-020-10252-8 doi (DE-627)SPR04521199X (SPR)s00344-020-10252-8-e DE-627 ger DE-627 rakwb eng 580 ASE 580 ASE 42.41 bkl 42.42 bkl Ndayambaza, Boniface verfasserin aut Genome-Wide Identification and Expression Analysis of the Barrel Medic (Medicago truncatula) and Alfalfa (Medicago sativa L.) Basic Helix-Loop-Helix Transcription Factor Family Under Salt and Drought Stresses 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2020 Abstract The basic helix loop helix (bHLH) transcription factor comprises one of the largest plant-specific transcriptional regulators in plant growth and development that response to biotic and abiotic stresses. Many members of bHLH play essential roles in the growth of root hair and response to drought, salt, and cold stresses. The family of bHLH genes has been found in many species; nevertheless, the barrel medic and alfalfa species still have a minute gap of bHLH new members thus far. This research aims to identify members of the bHLH family in barrel medic and alfalfa and elucidate their expression pattern level, network analysis, predictive 3D modeling and phylogenetic relationships. Here, we identified and characterized the bHLH gene family in both barrel medic and alfalfa plants and their genes expression response to drought, salinity, and cold stresses. A total of 159 MtbHLH and 133 MsbHLH genes were identified and characterized, divided into 18 subgroups and 17 subgroups, respectively. As a ubiquitous and popular method, neighbor-joining clustering was used. Based on the phylogenetic analyses, the VIII and IX subfamily and X subfamily were selected as the stress-related subfamily in these two species. The 154 MtbHLH genes were progressively distributed on the 8 chromosomes and 23 tandem duplicated genes, and 44 duplicated genes segments were detected in MtbHLH family. The analyses of gene ontology discovered the bHLH predominantly functions in protein and DNA binding in these two species. The results of Ka/Ks were < 1, which showed that the most orthologous of the bHLH gene values was found between A. thaliana and M. truncatula species. Remarkably, 7 MtbHLH and 10 MsbHLH genes were selected and validated with qRT-PCR after the treatment’s samples sampled under stressed abiotic conditions. The similar expression patterns between M. truncatula and M. sativa L have demonstrated identical expression patterns level in the root, and contrasting patterns in the stems and leaves were diverse. It was highlighted that the gene expression analyses of 17 bHLH genes were up-regulated to stresses, respectively, apart from some genes that were timely trended down-regulated to control (0 h). This study provided a concise understanding of the tissue specific of bHLH gene functions in genome-wide levels under drought, salt, and cold stresses. Our analyses provide the first insights onto the M. truncatula and M. sativa L evolution that contributes to molecular breeding for improving plant yield and stress tolerance. Barrel medic and alfalfa (dpeaa)DE-He213 transcription factors (dpeaa)DE-He213 Phylogenetic analysis (dpeaa)DE-He213 Abiotic stress (dpeaa)DE-He213 Gene expression analysis (dpeaa)DE-He213 Jin, Xiaoyu verfasserin aut Min, Xueyang verfasserin aut Lin, Xiaoshan verfasserin aut Yin, Xiaofan verfasserin aut Liu, Wenxian verfasserin aut Enthalten in Journal of plant growth regulation New York, NY : Springer, 1982 40(2020), 5 vom: 30. Okt., Seite 2058-2078 (DE-627)254630448 (DE-600)1462091-1 1435-8107 nnns volume:40 year:2020 number:5 day:30 month:10 pages:2058-2078 https://dx.doi.org/10.1007/s00344-020-10252-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.41 ASE 42.42 ASE AR 40 2020 5 30 10 2058-2078 |
| allfields_unstemmed |
10.1007/s00344-020-10252-8 doi (DE-627)SPR04521199X (SPR)s00344-020-10252-8-e DE-627 ger DE-627 rakwb eng 580 ASE 580 ASE 42.41 bkl 42.42 bkl Ndayambaza, Boniface verfasserin aut Genome-Wide Identification and Expression Analysis of the Barrel Medic (Medicago truncatula) and Alfalfa (Medicago sativa L.) Basic Helix-Loop-Helix Transcription Factor Family Under Salt and Drought Stresses 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2020 Abstract The basic helix loop helix (bHLH) transcription factor comprises one of the largest plant-specific transcriptional regulators in plant growth and development that response to biotic and abiotic stresses. Many members of bHLH play essential roles in the growth of root hair and response to drought, salt, and cold stresses. The family of bHLH genes has been found in many species; nevertheless, the barrel medic and alfalfa species still have a minute gap of bHLH new members thus far. This research aims to identify members of the bHLH family in barrel medic and alfalfa and elucidate their expression pattern level, network analysis, predictive 3D modeling and phylogenetic relationships. Here, we identified and characterized the bHLH gene family in both barrel medic and alfalfa plants and their genes expression response to drought, salinity, and cold stresses. A total of 159 MtbHLH and 133 MsbHLH genes were identified and characterized, divided into 18 subgroups and 17 subgroups, respectively. As a ubiquitous and popular method, neighbor-joining clustering was used. Based on the phylogenetic analyses, the VIII and IX subfamily and X subfamily were selected as the stress-related subfamily in these two species. The 154 MtbHLH genes were progressively distributed on the 8 chromosomes and 23 tandem duplicated genes, and 44 duplicated genes segments were detected in MtbHLH family. The analyses of gene ontology discovered the bHLH predominantly functions in protein and DNA binding in these two species. The results of Ka/Ks were < 1, which showed that the most orthologous of the bHLH gene values was found between A. thaliana and M. truncatula species. Remarkably, 7 MtbHLH and 10 MsbHLH genes were selected and validated with qRT-PCR after the treatment’s samples sampled under stressed abiotic conditions. The similar expression patterns between M. truncatula and M. sativa L have demonstrated identical expression patterns level in the root, and contrasting patterns in the stems and leaves were diverse. It was highlighted that the gene expression analyses of 17 bHLH genes were up-regulated to stresses, respectively, apart from some genes that were timely trended down-regulated to control (0 h). This study provided a concise understanding of the tissue specific of bHLH gene functions in genome-wide levels under drought, salt, and cold stresses. Our analyses provide the first insights onto the M. truncatula and M. sativa L evolution that contributes to molecular breeding for improving plant yield and stress tolerance. Barrel medic and alfalfa (dpeaa)DE-He213 transcription factors (dpeaa)DE-He213 Phylogenetic analysis (dpeaa)DE-He213 Abiotic stress (dpeaa)DE-He213 Gene expression analysis (dpeaa)DE-He213 Jin, Xiaoyu verfasserin aut Min, Xueyang verfasserin aut Lin, Xiaoshan verfasserin aut Yin, Xiaofan verfasserin aut Liu, Wenxian verfasserin aut Enthalten in Journal of plant growth regulation New York, NY : Springer, 1982 40(2020), 5 vom: 30. Okt., Seite 2058-2078 (DE-627)254630448 (DE-600)1462091-1 1435-8107 nnns volume:40 year:2020 number:5 day:30 month:10 pages:2058-2078 https://dx.doi.org/10.1007/s00344-020-10252-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.41 ASE 42.42 ASE AR 40 2020 5 30 10 2058-2078 |
| allfieldsGer |
10.1007/s00344-020-10252-8 doi (DE-627)SPR04521199X (SPR)s00344-020-10252-8-e DE-627 ger DE-627 rakwb eng 580 ASE 580 ASE 42.41 bkl 42.42 bkl Ndayambaza, Boniface verfasserin aut Genome-Wide Identification and Expression Analysis of the Barrel Medic (Medicago truncatula) and Alfalfa (Medicago sativa L.) Basic Helix-Loop-Helix Transcription Factor Family Under Salt and Drought Stresses 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2020 Abstract The basic helix loop helix (bHLH) transcription factor comprises one of the largest plant-specific transcriptional regulators in plant growth and development that response to biotic and abiotic stresses. Many members of bHLH play essential roles in the growth of root hair and response to drought, salt, and cold stresses. The family of bHLH genes has been found in many species; nevertheless, the barrel medic and alfalfa species still have a minute gap of bHLH new members thus far. This research aims to identify members of the bHLH family in barrel medic and alfalfa and elucidate their expression pattern level, network analysis, predictive 3D modeling and phylogenetic relationships. Here, we identified and characterized the bHLH gene family in both barrel medic and alfalfa plants and their genes expression response to drought, salinity, and cold stresses. A total of 159 MtbHLH and 133 MsbHLH genes were identified and characterized, divided into 18 subgroups and 17 subgroups, respectively. As a ubiquitous and popular method, neighbor-joining clustering was used. Based on the phylogenetic analyses, the VIII and IX subfamily and X subfamily were selected as the stress-related subfamily in these two species. The 154 MtbHLH genes were progressively distributed on the 8 chromosomes and 23 tandem duplicated genes, and 44 duplicated genes segments were detected in MtbHLH family. The analyses of gene ontology discovered the bHLH predominantly functions in protein and DNA binding in these two species. The results of Ka/Ks were < 1, which showed that the most orthologous of the bHLH gene values was found between A. thaliana and M. truncatula species. Remarkably, 7 MtbHLH and 10 MsbHLH genes were selected and validated with qRT-PCR after the treatment’s samples sampled under stressed abiotic conditions. The similar expression patterns between M. truncatula and M. sativa L have demonstrated identical expression patterns level in the root, and contrasting patterns in the stems and leaves were diverse. It was highlighted that the gene expression analyses of 17 bHLH genes were up-regulated to stresses, respectively, apart from some genes that were timely trended down-regulated to control (0 h). This study provided a concise understanding of the tissue specific of bHLH gene functions in genome-wide levels under drought, salt, and cold stresses. Our analyses provide the first insights onto the M. truncatula and M. sativa L evolution that contributes to molecular breeding for improving plant yield and stress tolerance. Barrel medic and alfalfa (dpeaa)DE-He213 transcription factors (dpeaa)DE-He213 Phylogenetic analysis (dpeaa)DE-He213 Abiotic stress (dpeaa)DE-He213 Gene expression analysis (dpeaa)DE-He213 Jin, Xiaoyu verfasserin aut Min, Xueyang verfasserin aut Lin, Xiaoshan verfasserin aut Yin, Xiaofan verfasserin aut Liu, Wenxian verfasserin aut Enthalten in Journal of plant growth regulation New York, NY : Springer, 1982 40(2020), 5 vom: 30. Okt., Seite 2058-2078 (DE-627)254630448 (DE-600)1462091-1 1435-8107 nnns volume:40 year:2020 number:5 day:30 month:10 pages:2058-2078 https://dx.doi.org/10.1007/s00344-020-10252-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.41 ASE 42.42 ASE AR 40 2020 5 30 10 2058-2078 |
| allfieldsSound |
10.1007/s00344-020-10252-8 doi (DE-627)SPR04521199X (SPR)s00344-020-10252-8-e DE-627 ger DE-627 rakwb eng 580 ASE 580 ASE 42.41 bkl 42.42 bkl Ndayambaza, Boniface verfasserin aut Genome-Wide Identification and Expression Analysis of the Barrel Medic (Medicago truncatula) and Alfalfa (Medicago sativa L.) Basic Helix-Loop-Helix Transcription Factor Family Under Salt and Drought Stresses 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2020 Abstract The basic helix loop helix (bHLH) transcription factor comprises one of the largest plant-specific transcriptional regulators in plant growth and development that response to biotic and abiotic stresses. Many members of bHLH play essential roles in the growth of root hair and response to drought, salt, and cold stresses. The family of bHLH genes has been found in many species; nevertheless, the barrel medic and alfalfa species still have a minute gap of bHLH new members thus far. This research aims to identify members of the bHLH family in barrel medic and alfalfa and elucidate their expression pattern level, network analysis, predictive 3D modeling and phylogenetic relationships. Here, we identified and characterized the bHLH gene family in both barrel medic and alfalfa plants and their genes expression response to drought, salinity, and cold stresses. A total of 159 MtbHLH and 133 MsbHLH genes were identified and characterized, divided into 18 subgroups and 17 subgroups, respectively. As a ubiquitous and popular method, neighbor-joining clustering was used. Based on the phylogenetic analyses, the VIII and IX subfamily and X subfamily were selected as the stress-related subfamily in these two species. The 154 MtbHLH genes were progressively distributed on the 8 chromosomes and 23 tandem duplicated genes, and 44 duplicated genes segments were detected in MtbHLH family. The analyses of gene ontology discovered the bHLH predominantly functions in protein and DNA binding in these two species. The results of Ka/Ks were < 1, which showed that the most orthologous of the bHLH gene values was found between A. thaliana and M. truncatula species. Remarkably, 7 MtbHLH and 10 MsbHLH genes were selected and validated with qRT-PCR after the treatment’s samples sampled under stressed abiotic conditions. The similar expression patterns between M. truncatula and M. sativa L have demonstrated identical expression patterns level in the root, and contrasting patterns in the stems and leaves were diverse. It was highlighted that the gene expression analyses of 17 bHLH genes were up-regulated to stresses, respectively, apart from some genes that were timely trended down-regulated to control (0 h). This study provided a concise understanding of the tissue specific of bHLH gene functions in genome-wide levels under drought, salt, and cold stresses. Our analyses provide the first insights onto the M. truncatula and M. sativa L evolution that contributes to molecular breeding for improving plant yield and stress tolerance. Barrel medic and alfalfa (dpeaa)DE-He213 transcription factors (dpeaa)DE-He213 Phylogenetic analysis (dpeaa)DE-He213 Abiotic stress (dpeaa)DE-He213 Gene expression analysis (dpeaa)DE-He213 Jin, Xiaoyu verfasserin aut Min, Xueyang verfasserin aut Lin, Xiaoshan verfasserin aut Yin, Xiaofan verfasserin aut Liu, Wenxian verfasserin aut Enthalten in Journal of plant growth regulation New York, NY : Springer, 1982 40(2020), 5 vom: 30. Okt., Seite 2058-2078 (DE-627)254630448 (DE-600)1462091-1 1435-8107 nnns volume:40 year:2020 number:5 day:30 month:10 pages:2058-2078 https://dx.doi.org/10.1007/s00344-020-10252-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.41 ASE 42.42 ASE AR 40 2020 5 30 10 2058-2078 |
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Ndayambaza, Boniface @@aut@@ Jin, Xiaoyu @@aut@@ Min, Xueyang @@aut@@ Lin, Xiaoshan @@aut@@ Yin, Xiaofan @@aut@@ Liu, Wenxian @@aut@@ |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR04521199X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519182143.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">211005s2020 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00344-020-10252-8</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR04521199X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s00344-020-10252-8-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">580</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">580</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">42.41</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">42.42</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Ndayambaza, Boniface</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Genome-Wide Identification and Expression Analysis of the Barrel Medic (Medicago truncatula) and Alfalfa (Medicago sativa L.) Basic Helix-Loop-Helix Transcription Factor Family Under Salt and Drought Stresses</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2020</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Springer Science+Business Media, LLC, part of Springer Nature 2020</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The basic helix loop helix (bHLH) transcription factor comprises one of the largest plant-specific transcriptional regulators in plant growth and development that response to biotic and abiotic stresses. Many members of bHLH play essential roles in the growth of root hair and response to drought, salt, and cold stresses. The family of bHLH genes has been found in many species; nevertheless, the barrel medic and alfalfa species still have a minute gap of bHLH new members thus far. This research aims to identify members of the bHLH family in barrel medic and alfalfa and elucidate their expression pattern level, network analysis, predictive 3D modeling and phylogenetic relationships. Here, we identified and characterized the bHLH gene family in both barrel medic and alfalfa plants and their genes expression response to drought, salinity, and cold stresses. A total of 159 MtbHLH and 133 MsbHLH genes were identified and characterized, divided into 18 subgroups and 17 subgroups, respectively. As a ubiquitous and popular method, neighbor-joining clustering was used. Based on the phylogenetic analyses, the VIII and IX subfamily and X subfamily were selected as the stress-related subfamily in these two species. The 154 MtbHLH genes were progressively distributed on the 8 chromosomes and 23 tandem duplicated genes, and 44 duplicated genes segments were detected in MtbHLH family. The analyses of gene ontology discovered the bHLH predominantly functions in protein and DNA binding in these two species. The results of Ka/Ks were < 1, which showed that the most orthologous of the bHLH gene values was found between A. thaliana and M. truncatula species. Remarkably, 7 MtbHLH and 10 MsbHLH genes were selected and validated with qRT-PCR after the treatment’s samples sampled under stressed abiotic conditions. The similar expression patterns between M. truncatula and M. sativa L have demonstrated identical expression patterns level in the root, and contrasting patterns in the stems and leaves were diverse. It was highlighted that the gene expression analyses of 17 bHLH genes were up-regulated to stresses, respectively, apart from some genes that were timely trended down-regulated to control (0 h). This study provided a concise understanding of the tissue specific of bHLH gene functions in genome-wide levels under drought, salt, and cold stresses. 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|
| author |
Ndayambaza, Boniface |
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Ndayambaza, Boniface ddc 580 bkl 42.41 bkl 42.42 misc Barrel medic and alfalfa misc transcription factors misc Phylogenetic analysis misc Abiotic stress misc Gene expression analysis Genome-Wide Identification and Expression Analysis of the Barrel Medic (Medicago truncatula) and Alfalfa (Medicago sativa L.) Basic Helix-Loop-Helix Transcription Factor Family Under Salt and Drought Stresses |
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580 ASE 42.41 bkl 42.42 bkl Genome-Wide Identification and Expression Analysis of the Barrel Medic (Medicago truncatula) and Alfalfa (Medicago sativa L.) Basic Helix-Loop-Helix Transcription Factor Family Under Salt and Drought Stresses Barrel medic and alfalfa (dpeaa)DE-He213 transcription factors (dpeaa)DE-He213 Phylogenetic analysis (dpeaa)DE-He213 Abiotic stress (dpeaa)DE-He213 Gene expression analysis (dpeaa)DE-He213 |
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ddc 580 bkl 42.41 bkl 42.42 misc Barrel medic and alfalfa misc transcription factors misc Phylogenetic analysis misc Abiotic stress misc Gene expression analysis |
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ddc 580 bkl 42.41 bkl 42.42 misc Barrel medic and alfalfa misc transcription factors misc Phylogenetic analysis misc Abiotic stress misc Gene expression analysis |
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ddc 580 bkl 42.41 bkl 42.42 misc Barrel medic and alfalfa misc transcription factors misc Phylogenetic analysis misc Abiotic stress misc Gene expression analysis |
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Journal of plant growth regulation |
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Genome-Wide Identification and Expression Analysis of the Barrel Medic (Medicago truncatula) and Alfalfa (Medicago sativa L.) Basic Helix-Loop-Helix Transcription Factor Family Under Salt and Drought Stresses |
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genome-wide identification and expression analysis of the barrel medic (medicago truncatula) and alfalfa (medicago sativa l.) basic helix-loop-helix transcription factor family under salt and drought stresses |
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Genome-Wide Identification and Expression Analysis of the Barrel Medic (Medicago truncatula) and Alfalfa (Medicago sativa L.) Basic Helix-Loop-Helix Transcription Factor Family Under Salt and Drought Stresses |
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Abstract The basic helix loop helix (bHLH) transcription factor comprises one of the largest plant-specific transcriptional regulators in plant growth and development that response to biotic and abiotic stresses. Many members of bHLH play essential roles in the growth of root hair and response to drought, salt, and cold stresses. The family of bHLH genes has been found in many species; nevertheless, the barrel medic and alfalfa species still have a minute gap of bHLH new members thus far. This research aims to identify members of the bHLH family in barrel medic and alfalfa and elucidate their expression pattern level, network analysis, predictive 3D modeling and phylogenetic relationships. Here, we identified and characterized the bHLH gene family in both barrel medic and alfalfa plants and their genes expression response to drought, salinity, and cold stresses. A total of 159 MtbHLH and 133 MsbHLH genes were identified and characterized, divided into 18 subgroups and 17 subgroups, respectively. As a ubiquitous and popular method, neighbor-joining clustering was used. Based on the phylogenetic analyses, the VIII and IX subfamily and X subfamily were selected as the stress-related subfamily in these two species. The 154 MtbHLH genes were progressively distributed on the 8 chromosomes and 23 tandem duplicated genes, and 44 duplicated genes segments were detected in MtbHLH family. The analyses of gene ontology discovered the bHLH predominantly functions in protein and DNA binding in these two species. The results of Ka/Ks were < 1, which showed that the most orthologous of the bHLH gene values was found between A. thaliana and M. truncatula species. Remarkably, 7 MtbHLH and 10 MsbHLH genes were selected and validated with qRT-PCR after the treatment’s samples sampled under stressed abiotic conditions. The similar expression patterns between M. truncatula and M. sativa L have demonstrated identical expression patterns level in the root, and contrasting patterns in the stems and leaves were diverse. It was highlighted that the gene expression analyses of 17 bHLH genes were up-regulated to stresses, respectively, apart from some genes that were timely trended down-regulated to control (0 h). This study provided a concise understanding of the tissue specific of bHLH gene functions in genome-wide levels under drought, salt, and cold stresses. Our analyses provide the first insights onto the M. truncatula and M. sativa L evolution that contributes to molecular breeding for improving plant yield and stress tolerance. © Springer Science+Business Media, LLC, part of Springer Nature 2020 |
| abstractGer |
Abstract The basic helix loop helix (bHLH) transcription factor comprises one of the largest plant-specific transcriptional regulators in plant growth and development that response to biotic and abiotic stresses. Many members of bHLH play essential roles in the growth of root hair and response to drought, salt, and cold stresses. The family of bHLH genes has been found in many species; nevertheless, the barrel medic and alfalfa species still have a minute gap of bHLH new members thus far. This research aims to identify members of the bHLH family in barrel medic and alfalfa and elucidate their expression pattern level, network analysis, predictive 3D modeling and phylogenetic relationships. Here, we identified and characterized the bHLH gene family in both barrel medic and alfalfa plants and their genes expression response to drought, salinity, and cold stresses. A total of 159 MtbHLH and 133 MsbHLH genes were identified and characterized, divided into 18 subgroups and 17 subgroups, respectively. As a ubiquitous and popular method, neighbor-joining clustering was used. Based on the phylogenetic analyses, the VIII and IX subfamily and X subfamily were selected as the stress-related subfamily in these two species. The 154 MtbHLH genes were progressively distributed on the 8 chromosomes and 23 tandem duplicated genes, and 44 duplicated genes segments were detected in MtbHLH family. The analyses of gene ontology discovered the bHLH predominantly functions in protein and DNA binding in these two species. The results of Ka/Ks were < 1, which showed that the most orthologous of the bHLH gene values was found between A. thaliana and M. truncatula species. Remarkably, 7 MtbHLH and 10 MsbHLH genes were selected and validated with qRT-PCR after the treatment’s samples sampled under stressed abiotic conditions. The similar expression patterns between M. truncatula and M. sativa L have demonstrated identical expression patterns level in the root, and contrasting patterns in the stems and leaves were diverse. It was highlighted that the gene expression analyses of 17 bHLH genes were up-regulated to stresses, respectively, apart from some genes that were timely trended down-regulated to control (0 h). This study provided a concise understanding of the tissue specific of bHLH gene functions in genome-wide levels under drought, salt, and cold stresses. Our analyses provide the first insights onto the M. truncatula and M. sativa L evolution that contributes to molecular breeding for improving plant yield and stress tolerance. © Springer Science+Business Media, LLC, part of Springer Nature 2020 |
| abstract_unstemmed |
Abstract The basic helix loop helix (bHLH) transcription factor comprises one of the largest plant-specific transcriptional regulators in plant growth and development that response to biotic and abiotic stresses. Many members of bHLH play essential roles in the growth of root hair and response to drought, salt, and cold stresses. The family of bHLH genes has been found in many species; nevertheless, the barrel medic and alfalfa species still have a minute gap of bHLH new members thus far. This research aims to identify members of the bHLH family in barrel medic and alfalfa and elucidate their expression pattern level, network analysis, predictive 3D modeling and phylogenetic relationships. Here, we identified and characterized the bHLH gene family in both barrel medic and alfalfa plants and their genes expression response to drought, salinity, and cold stresses. A total of 159 MtbHLH and 133 MsbHLH genes were identified and characterized, divided into 18 subgroups and 17 subgroups, respectively. As a ubiquitous and popular method, neighbor-joining clustering was used. Based on the phylogenetic analyses, the VIII and IX subfamily and X subfamily were selected as the stress-related subfamily in these two species. The 154 MtbHLH genes were progressively distributed on the 8 chromosomes and 23 tandem duplicated genes, and 44 duplicated genes segments were detected in MtbHLH family. The analyses of gene ontology discovered the bHLH predominantly functions in protein and DNA binding in these two species. The results of Ka/Ks were < 1, which showed that the most orthologous of the bHLH gene values was found between A. thaliana and M. truncatula species. Remarkably, 7 MtbHLH and 10 MsbHLH genes were selected and validated with qRT-PCR after the treatment’s samples sampled under stressed abiotic conditions. The similar expression patterns between M. truncatula and M. sativa L have demonstrated identical expression patterns level in the root, and contrasting patterns in the stems and leaves were diverse. It was highlighted that the gene expression analyses of 17 bHLH genes were up-regulated to stresses, respectively, apart from some genes that were timely trended down-regulated to control (0 h). This study provided a concise understanding of the tissue specific of bHLH gene functions in genome-wide levels under drought, salt, and cold stresses. Our analyses provide the first insights onto the M. truncatula and M. sativa L evolution that contributes to molecular breeding for improving plant yield and stress tolerance. © Springer Science+Business Media, LLC, part of Springer Nature 2020 |
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Genome-Wide Identification and Expression Analysis of the Barrel Medic (Medicago truncatula) and Alfalfa (Medicago sativa L.) Basic Helix-Loop-Helix Transcription Factor Family Under Salt and Drought Stresses |
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| score |
7.3986187 |