Nature of the tapetum-specific promoter is crucial for generation of rice transgenics possessing a lethal barnase gene

Abstract The indispensable requirement for the barnase–barstar based hybrid seed production technology is the availability of a tightly regulated tapetum-specific promoter. Until now, the specificity of literature reported tapetum-specific promoters are mainly characterized based on the fusion of no...
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Gespeichert in:

Autor*in:	Kumar, Pravin [verfasserIn] Purty, Ram Singh [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	Hybrid seed production Male sterile line gene Promoter–reporter fusion approach Tapetum-specific promoter

Anmerkung:	© Korean Society of Crop Science (KSCS) 2021

Übergeordnetes Werk:	Enthalten in: Journal of crop science and biotechnology - Seoul : Korean Soc. of Crop Science, 2009, 24(2021), 5 vom: 05. Juli, Seite 579-587
Übergeordnetes Werk:	volume:24 ; year:2021 ; number:5 ; day:05 ; month:07 ; pages:579-587

Links:	Volltext

DOI / URN:	10.1007/s12892-021-00106-4

Katalog-ID:	SPR045493987

Internformat


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245	1	0	\|a Nature of the tapetum-specific promoter is crucial for generation of rice transgenics possessing a lethal barnase gene
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520			\|a Abstract The indispensable requirement for the barnase–barstar based hybrid seed production technology is the availability of a tightly regulated tapetum-specific promoter. Until now, the specificity of literature reported tapetum-specific promoters are mainly characterized based on the fusion of non-lethal GUS/GFP gene as promoter–reporter fusion approach. In our previous report, the tapetum-specific OsG6b promoter had successfully generated 100% male sterile barnase line, whereas the OsRTS promoter (from indica rice cultivar IR64) was leaky in nature. In continuation, our observation with another two tapetum-specific promoters is reported here. Two distinct phenotypes with no male sterility were observed in the OsFbox promoter barnase lines due to leaky expression, whereas almost half of the OsYY2 promoter lines were male sterile. Based on our results, it is clear that the tapetum-specific promoters characterized based on the fusion of non-lethal GUS/GFP gene regulate the non-specific lethal barnase gene differently. Therefore, the ideal way of characterizing the tapetum-specific promoter for hybrid seed production should be a lethal gene such as barnase instead of non-lethal GUS/GFP gene.
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912			\|a GBV_ILN_120
912			\|a GBV_ILN_138
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_152
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_250
912			\|a GBV_ILN_281
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
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912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
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912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
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912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2056
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912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2144
912			\|a GBV_ILN_2147
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912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
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912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2446
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912			\|a GBV_ILN_2472
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912			\|a GBV_ILN_2522
912			\|a GBV_ILN_2548
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
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912			\|a GBV_ILN_4125
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author_variant	p k pk r s p rs rsp
matchkey_str	article:20058276:2021----::auefhtptmpcfcrmtrsrcafreeainfierngncp
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allfields	10.1007/s12892-021-00106-4 doi (DE-627)SPR045493987 (SPR)s12892-021-00106-4-e DE-627 ger DE-627 rakwb eng 630 640 ASE Kumar, Pravin verfasserin aut Nature of the tapetum-specific promoter is crucial for generation of rice transgenics possessing a lethal barnase gene 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Korean Society of Crop Science (KSCS) 2021 Abstract The indispensable requirement for the barnase–barstar based hybrid seed production technology is the availability of a tightly regulated tapetum-specific promoter. Until now, the specificity of literature reported tapetum-specific promoters are mainly characterized based on the fusion of non-lethal GUS/GFP gene as promoter–reporter fusion approach. In our previous report, the tapetum-specific OsG6b promoter had successfully generated 100% male sterile barnase line, whereas the OsRTS promoter (from indica rice cultivar IR64) was leaky in nature. In continuation, our observation with another two tapetum-specific promoters is reported here. Two distinct phenotypes with no male sterility were observed in the OsFbox promoter barnase lines due to leaky expression, whereas almost half of the OsYY2 promoter lines were male sterile. Based on our results, it is clear that the tapetum-specific promoters characterized based on the fusion of non-lethal GUS/GFP gene regulate the non-specific lethal barnase gene differently. Therefore, the ideal way of characterizing the tapetum-specific promoter for hybrid seed production should be a lethal gene such as barnase instead of non-lethal GUS/GFP gene. Hybrid seed production (dpeaa)DE-He213 Male sterile line (dpeaa)DE-He213 gene (dpeaa)DE-He213 Promoter–reporter fusion approach (dpeaa)DE-He213 Tapetum-specific promoter (dpeaa)DE-He213 Purty, Ram Singh verfasserin aut Enthalten in Journal of crop science and biotechnology Seoul : Korean Soc. of Crop Science, 2009 24(2021), 5 vom: 05. Juli, Seite 579-587 (DE-627)617512175 (DE-600)2534833-4 2005-8276 nnns volume:24 year:2021 number:5 day:05 month:07 pages:579-587 https://dx.doi.org/10.1007/s12892-021-00106-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 24 2021 5 05 07 579-587
spelling	10.1007/s12892-021-00106-4 doi (DE-627)SPR045493987 (SPR)s12892-021-00106-4-e DE-627 ger DE-627 rakwb eng 630 640 ASE Kumar, Pravin verfasserin aut Nature of the tapetum-specific promoter is crucial for generation of rice transgenics possessing a lethal barnase gene 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Korean Society of Crop Science (KSCS) 2021 Abstract The indispensable requirement for the barnase–barstar based hybrid seed production technology is the availability of a tightly regulated tapetum-specific promoter. Until now, the specificity of literature reported tapetum-specific promoters are mainly characterized based on the fusion of non-lethal GUS/GFP gene as promoter–reporter fusion approach. In our previous report, the tapetum-specific OsG6b promoter had successfully generated 100% male sterile barnase line, whereas the OsRTS promoter (from indica rice cultivar IR64) was leaky in nature. In continuation, our observation with another two tapetum-specific promoters is reported here. Two distinct phenotypes with no male sterility were observed in the OsFbox promoter barnase lines due to leaky expression, whereas almost half of the OsYY2 promoter lines were male sterile. Based on our results, it is clear that the tapetum-specific promoters characterized based on the fusion of non-lethal GUS/GFP gene regulate the non-specific lethal barnase gene differently. Therefore, the ideal way of characterizing the tapetum-specific promoter for hybrid seed production should be a lethal gene such as barnase instead of non-lethal GUS/GFP gene. Hybrid seed production (dpeaa)DE-He213 Male sterile line (dpeaa)DE-He213 gene (dpeaa)DE-He213 Promoter–reporter fusion approach (dpeaa)DE-He213 Tapetum-specific promoter (dpeaa)DE-He213 Purty, Ram Singh verfasserin aut Enthalten in Journal of crop science and biotechnology Seoul : Korean Soc. of Crop Science, 2009 24(2021), 5 vom: 05. Juli, Seite 579-587 (DE-627)617512175 (DE-600)2534833-4 2005-8276 nnns volume:24 year:2021 number:5 day:05 month:07 pages:579-587 https://dx.doi.org/10.1007/s12892-021-00106-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 24 2021 5 05 07 579-587
allfields_unstemmed	10.1007/s12892-021-00106-4 doi (DE-627)SPR045493987 (SPR)s12892-021-00106-4-e DE-627 ger DE-627 rakwb eng 630 640 ASE Kumar, Pravin verfasserin aut Nature of the tapetum-specific promoter is crucial for generation of rice transgenics possessing a lethal barnase gene 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Korean Society of Crop Science (KSCS) 2021 Abstract The indispensable requirement for the barnase–barstar based hybrid seed production technology is the availability of a tightly regulated tapetum-specific promoter. Until now, the specificity of literature reported tapetum-specific promoters are mainly characterized based on the fusion of non-lethal GUS/GFP gene as promoter–reporter fusion approach. In our previous report, the tapetum-specific OsG6b promoter had successfully generated 100% male sterile barnase line, whereas the OsRTS promoter (from indica rice cultivar IR64) was leaky in nature. In continuation, our observation with another two tapetum-specific promoters is reported here. Two distinct phenotypes with no male sterility were observed in the OsFbox promoter barnase lines due to leaky expression, whereas almost half of the OsYY2 promoter lines were male sterile. Based on our results, it is clear that the tapetum-specific promoters characterized based on the fusion of non-lethal GUS/GFP gene regulate the non-specific lethal barnase gene differently. Therefore, the ideal way of characterizing the tapetum-specific promoter for hybrid seed production should be a lethal gene such as barnase instead of non-lethal GUS/GFP gene. Hybrid seed production (dpeaa)DE-He213 Male sterile line (dpeaa)DE-He213 gene (dpeaa)DE-He213 Promoter–reporter fusion approach (dpeaa)DE-He213 Tapetum-specific promoter (dpeaa)DE-He213 Purty, Ram Singh verfasserin aut Enthalten in Journal of crop science and biotechnology Seoul : Korean Soc. of Crop Science, 2009 24(2021), 5 vom: 05. Juli, Seite 579-587 (DE-627)617512175 (DE-600)2534833-4 2005-8276 nnns volume:24 year:2021 number:5 day:05 month:07 pages:579-587 https://dx.doi.org/10.1007/s12892-021-00106-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 24 2021 5 05 07 579-587
allfieldsGer	10.1007/s12892-021-00106-4 doi (DE-627)SPR045493987 (SPR)s12892-021-00106-4-e DE-627 ger DE-627 rakwb eng 630 640 ASE Kumar, Pravin verfasserin aut Nature of the tapetum-specific promoter is crucial for generation of rice transgenics possessing a lethal barnase gene 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Korean Society of Crop Science (KSCS) 2021 Abstract The indispensable requirement for the barnase–barstar based hybrid seed production technology is the availability of a tightly regulated tapetum-specific promoter. Until now, the specificity of literature reported tapetum-specific promoters are mainly characterized based on the fusion of non-lethal GUS/GFP gene as promoter–reporter fusion approach. In our previous report, the tapetum-specific OsG6b promoter had successfully generated 100% male sterile barnase line, whereas the OsRTS promoter (from indica rice cultivar IR64) was leaky in nature. In continuation, our observation with another two tapetum-specific promoters is reported here. Two distinct phenotypes with no male sterility were observed in the OsFbox promoter barnase lines due to leaky expression, whereas almost half of the OsYY2 promoter lines were male sterile. Based on our results, it is clear that the tapetum-specific promoters characterized based on the fusion of non-lethal GUS/GFP gene regulate the non-specific lethal barnase gene differently. Therefore, the ideal way of characterizing the tapetum-specific promoter for hybrid seed production should be a lethal gene such as barnase instead of non-lethal GUS/GFP gene. Hybrid seed production (dpeaa)DE-He213 Male sterile line (dpeaa)DE-He213 gene (dpeaa)DE-He213 Promoter–reporter fusion approach (dpeaa)DE-He213 Tapetum-specific promoter (dpeaa)DE-He213 Purty, Ram Singh verfasserin aut Enthalten in Journal of crop science and biotechnology Seoul : Korean Soc. of Crop Science, 2009 24(2021), 5 vom: 05. Juli, Seite 579-587 (DE-627)617512175 (DE-600)2534833-4 2005-8276 nnns volume:24 year:2021 number:5 day:05 month:07 pages:579-587 https://dx.doi.org/10.1007/s12892-021-00106-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 24 2021 5 05 07 579-587
allfieldsSound	10.1007/s12892-021-00106-4 doi (DE-627)SPR045493987 (SPR)s12892-021-00106-4-e DE-627 ger DE-627 rakwb eng 630 640 ASE Kumar, Pravin verfasserin aut Nature of the tapetum-specific promoter is crucial for generation of rice transgenics possessing a lethal barnase gene 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Korean Society of Crop Science (KSCS) 2021 Abstract The indispensable requirement for the barnase–barstar based hybrid seed production technology is the availability of a tightly regulated tapetum-specific promoter. Until now, the specificity of literature reported tapetum-specific promoters are mainly characterized based on the fusion of non-lethal GUS/GFP gene as promoter–reporter fusion approach. In our previous report, the tapetum-specific OsG6b promoter had successfully generated 100% male sterile barnase line, whereas the OsRTS promoter (from indica rice cultivar IR64) was leaky in nature. In continuation, our observation with another two tapetum-specific promoters is reported here. Two distinct phenotypes with no male sterility were observed in the OsFbox promoter barnase lines due to leaky expression, whereas almost half of the OsYY2 promoter lines were male sterile. Based on our results, it is clear that the tapetum-specific promoters characterized based on the fusion of non-lethal GUS/GFP gene regulate the non-specific lethal barnase gene differently. Therefore, the ideal way of characterizing the tapetum-specific promoter for hybrid seed production should be a lethal gene such as barnase instead of non-lethal GUS/GFP gene. Hybrid seed production (dpeaa)DE-He213 Male sterile line (dpeaa)DE-He213 gene (dpeaa)DE-He213 Promoter–reporter fusion approach (dpeaa)DE-He213 Tapetum-specific promoter (dpeaa)DE-He213 Purty, Ram Singh verfasserin aut Enthalten in Journal of crop science and biotechnology Seoul : Korean Soc. of Crop Science, 2009 24(2021), 5 vom: 05. Juli, Seite 579-587 (DE-627)617512175 (DE-600)2534833-4 2005-8276 nnns volume:24 year:2021 number:5 day:05 month:07 pages:579-587 https://dx.doi.org/10.1007/s12892-021-00106-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 24 2021 5 05 07 579-587
language	English
source	Enthalten in Journal of crop science and biotechnology 24(2021), 5 vom: 05. Juli, Seite 579-587 volume:24 year:2021 number:5 day:05 month:07 pages:579-587
sourceStr	Enthalten in Journal of crop science and biotechnology 24(2021), 5 vom: 05. Juli, Seite 579-587 volume:24 year:2021 number:5 day:05 month:07 pages:579-587
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institution	findex.gbv.de
topic_facet	Hybrid seed production Male sterile line gene Promoter–reporter fusion approach Tapetum-specific promoter
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container_title	Journal of crop science and biotechnology
authorswithroles_txt_mv	Kumar, Pravin @@aut@@ Purty, Ram Singh @@aut@@
publishDateDaySort_date	2021-07-05T00:00:00Z
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author	Kumar, Pravin
spellingShingle	Kumar, Pravin ddc 630 misc Hybrid seed production misc Male sterile line misc gene misc Promoter–reporter fusion approach misc Tapetum-specific promoter Nature of the tapetum-specific promoter is crucial for generation of rice transgenics possessing a lethal barnase gene
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topic_title	630 640 ASE Nature of the tapetum-specific promoter is crucial for generation of rice transgenics possessing a lethal barnase gene Hybrid seed production (dpeaa)DE-He213 Male sterile line (dpeaa)DE-He213 gene (dpeaa)DE-He213 Promoter–reporter fusion approach (dpeaa)DE-He213 Tapetum-specific promoter (dpeaa)DE-He213
topic	ddc 630 misc Hybrid seed production misc Male sterile line misc gene misc Promoter–reporter fusion approach misc Tapetum-specific promoter
topic_unstemmed	ddc 630 misc Hybrid seed production misc Male sterile line misc gene misc Promoter–reporter fusion approach misc Tapetum-specific promoter
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title	Nature of the tapetum-specific promoter is crucial for generation of rice transgenics possessing a lethal barnase gene
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title_full	Nature of the tapetum-specific promoter is crucial for generation of rice transgenics possessing a lethal barnase gene
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title_sort	nature of the tapetum-specific promoter is crucial for generation of rice transgenics possessing a lethal barnase gene
title_auth	Nature of the tapetum-specific promoter is crucial for generation of rice transgenics possessing a lethal barnase gene
abstract	Abstract The indispensable requirement for the barnase–barstar based hybrid seed production technology is the availability of a tightly regulated tapetum-specific promoter. Until now, the specificity of literature reported tapetum-specific promoters are mainly characterized based on the fusion of non-lethal GUS/GFP gene as promoter–reporter fusion approach. In our previous report, the tapetum-specific OsG6b promoter had successfully generated 100% male sterile barnase line, whereas the OsRTS promoter (from indica rice cultivar IR64) was leaky in nature. In continuation, our observation with another two tapetum-specific promoters is reported here. Two distinct phenotypes with no male sterility were observed in the OsFbox promoter barnase lines due to leaky expression, whereas almost half of the OsYY2 promoter lines were male sterile. Based on our results, it is clear that the tapetum-specific promoters characterized based on the fusion of non-lethal GUS/GFP gene regulate the non-specific lethal barnase gene differently. Therefore, the ideal way of characterizing the tapetum-specific promoter for hybrid seed production should be a lethal gene such as barnase instead of non-lethal GUS/GFP gene. © Korean Society of Crop Science (KSCS) 2021
abstractGer	Abstract The indispensable requirement for the barnase–barstar based hybrid seed production technology is the availability of a tightly regulated tapetum-specific promoter. Until now, the specificity of literature reported tapetum-specific promoters are mainly characterized based on the fusion of non-lethal GUS/GFP gene as promoter–reporter fusion approach. In our previous report, the tapetum-specific OsG6b promoter had successfully generated 100% male sterile barnase line, whereas the OsRTS promoter (from indica rice cultivar IR64) was leaky in nature. In continuation, our observation with another two tapetum-specific promoters is reported here. Two distinct phenotypes with no male sterility were observed in the OsFbox promoter barnase lines due to leaky expression, whereas almost half of the OsYY2 promoter lines were male sterile. Based on our results, it is clear that the tapetum-specific promoters characterized based on the fusion of non-lethal GUS/GFP gene regulate the non-specific lethal barnase gene differently. Therefore, the ideal way of characterizing the tapetum-specific promoter for hybrid seed production should be a lethal gene such as barnase instead of non-lethal GUS/GFP gene. © Korean Society of Crop Science (KSCS) 2021
abstract_unstemmed	Abstract The indispensable requirement for the barnase–barstar based hybrid seed production technology is the availability of a tightly regulated tapetum-specific promoter. Until now, the specificity of literature reported tapetum-specific promoters are mainly characterized based on the fusion of non-lethal GUS/GFP gene as promoter–reporter fusion approach. In our previous report, the tapetum-specific OsG6b promoter had successfully generated 100% male sterile barnase line, whereas the OsRTS promoter (from indica rice cultivar IR64) was leaky in nature. In continuation, our observation with another two tapetum-specific promoters is reported here. Two distinct phenotypes with no male sterility were observed in the OsFbox promoter barnase lines due to leaky expression, whereas almost half of the OsYY2 promoter lines were male sterile. Based on our results, it is clear that the tapetum-specific promoters characterized based on the fusion of non-lethal GUS/GFP gene regulate the non-specific lethal barnase gene differently. Therefore, the ideal way of characterizing the tapetum-specific promoter for hybrid seed production should be a lethal gene such as barnase instead of non-lethal GUS/GFP gene. © Korean Society of Crop Science (KSCS) 2021
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container_issue	5
title_short	Nature of the tapetum-specific promoter is crucial for generation of rice transgenics possessing a lethal barnase gene
url	https://dx.doi.org/10.1007/s12892-021-00106-4
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author2	Purty, Ram Singh
author2Str	Purty, Ram Singh
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doi_str	10.1007/s12892-021-00106-4
up_date	2024-07-03T16:22:10.801Z
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Nature of the tapetum-specific promoter is crucial for generation of rice transgenics possessing a lethal barnase gene

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