Evaluation of milk deterioration using simple biosensor
Abstract In this study, a simple pH-colorimetric film was developed to detect the spoilage of pasteurized milk. The biosensor was fabricated by incorporating the pH-sensitive anthocyanins (AC) extracted from blue pea (Clitoria ternatea L.) flower into a starch-based edible film. The color responses...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Cho, Tong-Fei [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2021 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021 |
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| Übergeordnetes Werk: |
Enthalten in: Sensing and instrumentation for food quality and safety - New York, NY : Springer, 2007, 16(2021), 1 vom: 09. Sept., Seite 258-268 |
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| Übergeordnetes Werk: |
volume:16 ; year:2021 ; number:1 ; day:09 ; month:09 ; pages:258-268 |
| Links: |
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| DOI / URN: |
10.1007/s11694-021-01145-9 |
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SPR046017666 |
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| 520 | |a Abstract In this study, a simple pH-colorimetric film was developed to detect the spoilage of pasteurized milk. The biosensor was fabricated by incorporating the pH-sensitive anthocyanins (AC) extracted from blue pea (Clitoria ternatea L.) flower into a starch-based edible film. The color responses of both AC aqueous extracts and edible films incorporated with different AC concentrations towards different pH buffers (pH 2–11) were measured using Ultraviolet–visible (UV–Vis) spectrophotometer and colorimeter, respectively. Based on the results, there were significant differences (p < 0.05) for both total color changes (∆E) and L*a*b color system of the pH-sensitive edible film in different pH buffers. It indicated that the AC incorporated edible films were able to exhibit color changes over a wide range of pH. The potential application of the pH-sensitive film in spoilage detection was then carried out. Color changes of the film in commercial pasteurized milk were measured at predetermined intervals (24, 48, and 72 h) under storage conditions of 25 °C. Meanwhile, milk samples were collected for the microbiological test [total plate count (TPC)]. Results showed that the pH-sensitive films were able to detect pH changes of pasteurized milk which was associated with milk spoilage with visually observable color changes from deep blue to a light blue and showed a significant ∆E (p < 0.05) of 19.95 ± 0.009. The ∆E of the films in spoilage detection has a significant correlation with the pH changes of milk (R = − 0.9932) and TPC with a desirable coefficient (R = 0.9918). Therefore, the pH-sensitive edible film can provide a highly sensitive and non-destructive way of monitoring the food quality or spoilage without creating safety issues in the food industry. | ||
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10.1007/s11694-021-01145-9 doi (DE-627)SPR046017666 (SPR)s11694-021-01145-9-e DE-627 ger DE-627 rakwb eng Cho, Tong-Fei verfasserin aut Evaluation of milk deterioration using simple biosensor 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021 Abstract In this study, a simple pH-colorimetric film was developed to detect the spoilage of pasteurized milk. The biosensor was fabricated by incorporating the pH-sensitive anthocyanins (AC) extracted from blue pea (Clitoria ternatea L.) flower into a starch-based edible film. The color responses of both AC aqueous extracts and edible films incorporated with different AC concentrations towards different pH buffers (pH 2–11) were measured using Ultraviolet–visible (UV–Vis) spectrophotometer and colorimeter, respectively. Based on the results, there were significant differences (p < 0.05) for both total color changes (∆E) and L*a*b color system of the pH-sensitive edible film in different pH buffers. It indicated that the AC incorporated edible films were able to exhibit color changes over a wide range of pH. The potential application of the pH-sensitive film in spoilage detection was then carried out. Color changes of the film in commercial pasteurized milk were measured at predetermined intervals (24, 48, and 72 h) under storage conditions of 25 °C. Meanwhile, milk samples were collected for the microbiological test [total plate count (TPC)]. Results showed that the pH-sensitive films were able to detect pH changes of pasteurized milk which was associated with milk spoilage with visually observable color changes from deep blue to a light blue and showed a significant ∆E (p < 0.05) of 19.95 ± 0.009. The ∆E of the films in spoilage detection has a significant correlation with the pH changes of milk (R = − 0.9932) and TPC with a desirable coefficient (R = 0.9918). Therefore, the pH-sensitive edible film can provide a highly sensitive and non-destructive way of monitoring the food quality or spoilage without creating safety issues in the food industry. Anthocyanin (dpeaa)DE-He213 Blue pea flower (dpeaa)DE-He213 Color response (dpeaa)DE-He213 Milk deterioration (dpeaa)DE-He213 Edible film (dpeaa)DE-He213 Yassoralipour, Ali aut Lee, Yee-Ying aut Tang, Teck-Kim aut Lai, Oi-Ming aut Chong, Li-Choo aut Kuan, Chee-Hao aut Phuah, Eng-Tong (orcid)0000-0002-0334-2335 aut Enthalten in Sensing and instrumentation for food quality and safety New York, NY : Springer, 2007 16(2021), 1 vom: 09. Sept., Seite 258-268 (DE-627)528359339 (DE-600)2279937-0 1932-9954 nnns volume:16 year:2021 number:1 day:09 month:09 pages:258-268 https://dx.doi.org/10.1007/s11694-021-01145-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_285 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2014 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2059 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2153 GBV_ILN_2190 AR 16 2021 1 09 09 258-268 |
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10.1007/s11694-021-01145-9 doi (DE-627)SPR046017666 (SPR)s11694-021-01145-9-e DE-627 ger DE-627 rakwb eng Cho, Tong-Fei verfasserin aut Evaluation of milk deterioration using simple biosensor 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021 Abstract In this study, a simple pH-colorimetric film was developed to detect the spoilage of pasteurized milk. The biosensor was fabricated by incorporating the pH-sensitive anthocyanins (AC) extracted from blue pea (Clitoria ternatea L.) flower into a starch-based edible film. The color responses of both AC aqueous extracts and edible films incorporated with different AC concentrations towards different pH buffers (pH 2–11) were measured using Ultraviolet–visible (UV–Vis) spectrophotometer and colorimeter, respectively. Based on the results, there were significant differences (p < 0.05) for both total color changes (∆E) and L*a*b color system of the pH-sensitive edible film in different pH buffers. It indicated that the AC incorporated edible films were able to exhibit color changes over a wide range of pH. The potential application of the pH-sensitive film in spoilage detection was then carried out. Color changes of the film in commercial pasteurized milk were measured at predetermined intervals (24, 48, and 72 h) under storage conditions of 25 °C. Meanwhile, milk samples were collected for the microbiological test [total plate count (TPC)]. Results showed that the pH-sensitive films were able to detect pH changes of pasteurized milk which was associated with milk spoilage with visually observable color changes from deep blue to a light blue and showed a significant ∆E (p < 0.05) of 19.95 ± 0.009. The ∆E of the films in spoilage detection has a significant correlation with the pH changes of milk (R = − 0.9932) and TPC with a desirable coefficient (R = 0.9918). Therefore, the pH-sensitive edible film can provide a highly sensitive and non-destructive way of monitoring the food quality or spoilage without creating safety issues in the food industry. Anthocyanin (dpeaa)DE-He213 Blue pea flower (dpeaa)DE-He213 Color response (dpeaa)DE-He213 Milk deterioration (dpeaa)DE-He213 Edible film (dpeaa)DE-He213 Yassoralipour, Ali aut Lee, Yee-Ying aut Tang, Teck-Kim aut Lai, Oi-Ming aut Chong, Li-Choo aut Kuan, Chee-Hao aut Phuah, Eng-Tong (orcid)0000-0002-0334-2335 aut Enthalten in Sensing and instrumentation for food quality and safety New York, NY : Springer, 2007 16(2021), 1 vom: 09. Sept., Seite 258-268 (DE-627)528359339 (DE-600)2279937-0 1932-9954 nnns volume:16 year:2021 number:1 day:09 month:09 pages:258-268 https://dx.doi.org/10.1007/s11694-021-01145-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_285 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2014 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2059 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2153 GBV_ILN_2190 AR 16 2021 1 09 09 258-268 |
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10.1007/s11694-021-01145-9 doi (DE-627)SPR046017666 (SPR)s11694-021-01145-9-e DE-627 ger DE-627 rakwb eng Cho, Tong-Fei verfasserin aut Evaluation of milk deterioration using simple biosensor 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021 Abstract In this study, a simple pH-colorimetric film was developed to detect the spoilage of pasteurized milk. The biosensor was fabricated by incorporating the pH-sensitive anthocyanins (AC) extracted from blue pea (Clitoria ternatea L.) flower into a starch-based edible film. The color responses of both AC aqueous extracts and edible films incorporated with different AC concentrations towards different pH buffers (pH 2–11) were measured using Ultraviolet–visible (UV–Vis) spectrophotometer and colorimeter, respectively. Based on the results, there were significant differences (p < 0.05) for both total color changes (∆E) and L*a*b color system of the pH-sensitive edible film in different pH buffers. It indicated that the AC incorporated edible films were able to exhibit color changes over a wide range of pH. The potential application of the pH-sensitive film in spoilage detection was then carried out. Color changes of the film in commercial pasteurized milk were measured at predetermined intervals (24, 48, and 72 h) under storage conditions of 25 °C. Meanwhile, milk samples were collected for the microbiological test [total plate count (TPC)]. Results showed that the pH-sensitive films were able to detect pH changes of pasteurized milk which was associated with milk spoilage with visually observable color changes from deep blue to a light blue and showed a significant ∆E (p < 0.05) of 19.95 ± 0.009. The ∆E of the films in spoilage detection has a significant correlation with the pH changes of milk (R = − 0.9932) and TPC with a desirable coefficient (R = 0.9918). Therefore, the pH-sensitive edible film can provide a highly sensitive and non-destructive way of monitoring the food quality or spoilage without creating safety issues in the food industry. Anthocyanin (dpeaa)DE-He213 Blue pea flower (dpeaa)DE-He213 Color response (dpeaa)DE-He213 Milk deterioration (dpeaa)DE-He213 Edible film (dpeaa)DE-He213 Yassoralipour, Ali aut Lee, Yee-Ying aut Tang, Teck-Kim aut Lai, Oi-Ming aut Chong, Li-Choo aut Kuan, Chee-Hao aut Phuah, Eng-Tong (orcid)0000-0002-0334-2335 aut Enthalten in Sensing and instrumentation for food quality and safety New York, NY : Springer, 2007 16(2021), 1 vom: 09. Sept., Seite 258-268 (DE-627)528359339 (DE-600)2279937-0 1932-9954 nnns volume:16 year:2021 number:1 day:09 month:09 pages:258-268 https://dx.doi.org/10.1007/s11694-021-01145-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_285 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2014 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2059 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2153 GBV_ILN_2190 AR 16 2021 1 09 09 258-268 |
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10.1007/s11694-021-01145-9 doi (DE-627)SPR046017666 (SPR)s11694-021-01145-9-e DE-627 ger DE-627 rakwb eng Cho, Tong-Fei verfasserin aut Evaluation of milk deterioration using simple biosensor 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021 Abstract In this study, a simple pH-colorimetric film was developed to detect the spoilage of pasteurized milk. The biosensor was fabricated by incorporating the pH-sensitive anthocyanins (AC) extracted from blue pea (Clitoria ternatea L.) flower into a starch-based edible film. The color responses of both AC aqueous extracts and edible films incorporated with different AC concentrations towards different pH buffers (pH 2–11) were measured using Ultraviolet–visible (UV–Vis) spectrophotometer and colorimeter, respectively. Based on the results, there were significant differences (p < 0.05) for both total color changes (∆E) and L*a*b color system of the pH-sensitive edible film in different pH buffers. It indicated that the AC incorporated edible films were able to exhibit color changes over a wide range of pH. The potential application of the pH-sensitive film in spoilage detection was then carried out. Color changes of the film in commercial pasteurized milk were measured at predetermined intervals (24, 48, and 72 h) under storage conditions of 25 °C. Meanwhile, milk samples were collected for the microbiological test [total plate count (TPC)]. Results showed that the pH-sensitive films were able to detect pH changes of pasteurized milk which was associated with milk spoilage with visually observable color changes from deep blue to a light blue and showed a significant ∆E (p < 0.05) of 19.95 ± 0.009. The ∆E of the films in spoilage detection has a significant correlation with the pH changes of milk (R = − 0.9932) and TPC with a desirable coefficient (R = 0.9918). Therefore, the pH-sensitive edible film can provide a highly sensitive and non-destructive way of monitoring the food quality or spoilage without creating safety issues in the food industry. Anthocyanin (dpeaa)DE-He213 Blue pea flower (dpeaa)DE-He213 Color response (dpeaa)DE-He213 Milk deterioration (dpeaa)DE-He213 Edible film (dpeaa)DE-He213 Yassoralipour, Ali aut Lee, Yee-Ying aut Tang, Teck-Kim aut Lai, Oi-Ming aut Chong, Li-Choo aut Kuan, Chee-Hao aut Phuah, Eng-Tong (orcid)0000-0002-0334-2335 aut Enthalten in Sensing and instrumentation for food quality and safety New York, NY : Springer, 2007 16(2021), 1 vom: 09. Sept., Seite 258-268 (DE-627)528359339 (DE-600)2279937-0 1932-9954 nnns volume:16 year:2021 number:1 day:09 month:09 pages:258-268 https://dx.doi.org/10.1007/s11694-021-01145-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_285 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2014 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2059 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2153 GBV_ILN_2190 AR 16 2021 1 09 09 258-268 |
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10.1007/s11694-021-01145-9 doi (DE-627)SPR046017666 (SPR)s11694-021-01145-9-e DE-627 ger DE-627 rakwb eng Cho, Tong-Fei verfasserin aut Evaluation of milk deterioration using simple biosensor 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021 Abstract In this study, a simple pH-colorimetric film was developed to detect the spoilage of pasteurized milk. The biosensor was fabricated by incorporating the pH-sensitive anthocyanins (AC) extracted from blue pea (Clitoria ternatea L.) flower into a starch-based edible film. The color responses of both AC aqueous extracts and edible films incorporated with different AC concentrations towards different pH buffers (pH 2–11) were measured using Ultraviolet–visible (UV–Vis) spectrophotometer and colorimeter, respectively. Based on the results, there were significant differences (p < 0.05) for both total color changes (∆E) and L*a*b color system of the pH-sensitive edible film in different pH buffers. It indicated that the AC incorporated edible films were able to exhibit color changes over a wide range of pH. The potential application of the pH-sensitive film in spoilage detection was then carried out. Color changes of the film in commercial pasteurized milk were measured at predetermined intervals (24, 48, and 72 h) under storage conditions of 25 °C. Meanwhile, milk samples were collected for the microbiological test [total plate count (TPC)]. Results showed that the pH-sensitive films were able to detect pH changes of pasteurized milk which was associated with milk spoilage with visually observable color changes from deep blue to a light blue and showed a significant ∆E (p < 0.05) of 19.95 ± 0.009. The ∆E of the films in spoilage detection has a significant correlation with the pH changes of milk (R = − 0.9932) and TPC with a desirable coefficient (R = 0.9918). Therefore, the pH-sensitive edible film can provide a highly sensitive and non-destructive way of monitoring the food quality or spoilage without creating safety issues in the food industry. Anthocyanin (dpeaa)DE-He213 Blue pea flower (dpeaa)DE-He213 Color response (dpeaa)DE-He213 Milk deterioration (dpeaa)DE-He213 Edible film (dpeaa)DE-He213 Yassoralipour, Ali aut Lee, Yee-Ying aut Tang, Teck-Kim aut Lai, Oi-Ming aut Chong, Li-Choo aut Kuan, Chee-Hao aut Phuah, Eng-Tong (orcid)0000-0002-0334-2335 aut Enthalten in Sensing and instrumentation for food quality and safety New York, NY : Springer, 2007 16(2021), 1 vom: 09. Sept., Seite 258-268 (DE-627)528359339 (DE-600)2279937-0 1932-9954 nnns volume:16 year:2021 number:1 day:09 month:09 pages:258-268 https://dx.doi.org/10.1007/s11694-021-01145-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_285 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2014 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2059 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2153 GBV_ILN_2190 AR 16 2021 1 09 09 258-268 |
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Abstract In this study, a simple pH-colorimetric film was developed to detect the spoilage of pasteurized milk. The biosensor was fabricated by incorporating the pH-sensitive anthocyanins (AC) extracted from blue pea (Clitoria ternatea L.) flower into a starch-based edible film. The color responses of both AC aqueous extracts and edible films incorporated with different AC concentrations towards different pH buffers (pH 2–11) were measured using Ultraviolet–visible (UV–Vis) spectrophotometer and colorimeter, respectively. Based on the results, there were significant differences (p < 0.05) for both total color changes (∆E) and L*a*b color system of the pH-sensitive edible film in different pH buffers. It indicated that the AC incorporated edible films were able to exhibit color changes over a wide range of pH. The potential application of the pH-sensitive film in spoilage detection was then carried out. Color changes of the film in commercial pasteurized milk were measured at predetermined intervals (24, 48, and 72 h) under storage conditions of 25 °C. Meanwhile, milk samples were collected for the microbiological test [total plate count (TPC)]. Results showed that the pH-sensitive films were able to detect pH changes of pasteurized milk which was associated with milk spoilage with visually observable color changes from deep blue to a light blue and showed a significant ∆E (p < 0.05) of 19.95 ± 0.009. The ∆E of the films in spoilage detection has a significant correlation with the pH changes of milk (R = − 0.9932) and TPC with a desirable coefficient (R = 0.9918). Therefore, the pH-sensitive edible film can provide a highly sensitive and non-destructive way of monitoring the food quality or spoilage without creating safety issues in the food industry. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021 |
| abstractGer |
Abstract In this study, a simple pH-colorimetric film was developed to detect the spoilage of pasteurized milk. The biosensor was fabricated by incorporating the pH-sensitive anthocyanins (AC) extracted from blue pea (Clitoria ternatea L.) flower into a starch-based edible film. The color responses of both AC aqueous extracts and edible films incorporated with different AC concentrations towards different pH buffers (pH 2–11) were measured using Ultraviolet–visible (UV–Vis) spectrophotometer and colorimeter, respectively. Based on the results, there were significant differences (p < 0.05) for both total color changes (∆E) and L*a*b color system of the pH-sensitive edible film in different pH buffers. It indicated that the AC incorporated edible films were able to exhibit color changes over a wide range of pH. The potential application of the pH-sensitive film in spoilage detection was then carried out. Color changes of the film in commercial pasteurized milk were measured at predetermined intervals (24, 48, and 72 h) under storage conditions of 25 °C. Meanwhile, milk samples were collected for the microbiological test [total plate count (TPC)]. Results showed that the pH-sensitive films were able to detect pH changes of pasteurized milk which was associated with milk spoilage with visually observable color changes from deep blue to a light blue and showed a significant ∆E (p < 0.05) of 19.95 ± 0.009. The ∆E of the films in spoilage detection has a significant correlation with the pH changes of milk (R = − 0.9932) and TPC with a desirable coefficient (R = 0.9918). Therefore, the pH-sensitive edible film can provide a highly sensitive and non-destructive way of monitoring the food quality or spoilage without creating safety issues in the food industry. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021 |
| abstract_unstemmed |
Abstract In this study, a simple pH-colorimetric film was developed to detect the spoilage of pasteurized milk. The biosensor was fabricated by incorporating the pH-sensitive anthocyanins (AC) extracted from blue pea (Clitoria ternatea L.) flower into a starch-based edible film. The color responses of both AC aqueous extracts and edible films incorporated with different AC concentrations towards different pH buffers (pH 2–11) were measured using Ultraviolet–visible (UV–Vis) spectrophotometer and colorimeter, respectively. Based on the results, there were significant differences (p < 0.05) for both total color changes (∆E) and L*a*b color system of the pH-sensitive edible film in different pH buffers. It indicated that the AC incorporated edible films were able to exhibit color changes over a wide range of pH. The potential application of the pH-sensitive film in spoilage detection was then carried out. Color changes of the film in commercial pasteurized milk were measured at predetermined intervals (24, 48, and 72 h) under storage conditions of 25 °C. Meanwhile, milk samples were collected for the microbiological test [total plate count (TPC)]. Results showed that the pH-sensitive films were able to detect pH changes of pasteurized milk which was associated with milk spoilage with visually observable color changes from deep blue to a light blue and showed a significant ∆E (p < 0.05) of 19.95 ± 0.009. The ∆E of the films in spoilage detection has a significant correlation with the pH changes of milk (R = − 0.9932) and TPC with a desirable coefficient (R = 0.9918). Therefore, the pH-sensitive edible film can provide a highly sensitive and non-destructive way of monitoring the food quality or spoilage without creating safety issues in the food industry. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021 |
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Meanwhile, milk samples were collected for the microbiological test [total plate count (TPC)]. Results showed that the pH-sensitive films were able to detect pH changes of pasteurized milk which was associated with milk spoilage with visually observable color changes from deep blue to a light blue and showed a significant ∆E (p < 0.05) of 19.95 ± 0.009. The ∆E of the films in spoilage detection has a significant correlation with the pH changes of milk (R = − 0.9932) and TPC with a desirable coefficient (R = 0.9918). 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