Bioassay methods for extraction and detection of antifungal potential of the epicuticular layer of Theobroma cacao pods against the witches’ broom pathogen
Abstract The witches’ broom disease of cacao, caused by the fungus Moniliophthora perniciosa, is among the main diseases of cacao (Theobroma cacao L.), one of the most important cultures in the tropics, for which chemical control is not very effective. We suggested that the epicuticular wax layer of...
Ausführliche Beschreibung
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| Autor*in: |
Carvalho, Mariana R. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2021 |
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| Schlagwörter: |
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| Anmerkung: |
© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2021 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of plant pathology - [Cham] : Springer International Publishing, 1997, 104(2021), 1 vom: 26. Okt., Seite 343-348 |
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| Übergeordnetes Werk: |
volume:104 ; year:2021 ; number:1 ; day:26 ; month:10 ; pages:343-348 |
| Links: |
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| DOI / URN: |
10.1007/s42161-021-00945-8 |
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| Katalog-ID: |
SPR046315128 |
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| 520 | |a Abstract The witches’ broom disease of cacao, caused by the fungus Moniliophthora perniciosa, is among the main diseases of cacao (Theobroma cacao L.), one of the most important cultures in the tropics, for which chemical control is not very effective. We suggested that the epicuticular wax layer of cacao pods might have antifungal activity against Moniliophthora perniciosa. The objective of this article was to establish a protocol for extraction of the epicuticular layer of cacao pods and establish a bioassay to evaluate the in vitro antifungal activity of these epicuticular extracts on germination of M. perniciosa. First, we standardized the extraction protocol, and extracts of the epicuticular layer from standard, susceptible, and resistant cacao clones to WBDC were obtained. Then, we used an in vitro agar bioassay to evaluate the antifungal activity of the extracts against M. perniciosa basidiospores. The inhibition of the germination was highest (87.2%) for spores treated with the extracts from the resistant genotype. Treatment with extracts from ICS1 (susceptible genotype) did not significantly influence spore germination. We successfully extracted T. cacao wax and suggested that the epicuticular layer of cacao genotypes has antifungal molecules with inhibitory activity against the germination of M. perniciosa basidiospores in vitro. | ||
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| 650 | 4 | |a Witches’ broom disease |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Sena, Kaleandra F. |4 aut | |
| 700 | 1 | |a Lopes, Uilson V. |4 aut | |
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| 700 | 1 | |a dos Santos Filho, Lindolfo P. |4 aut | |
| 700 | 1 | |a Araújo, Saulo Andrade |4 aut | |
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| 700 | 1 | |a Gramacho, Karina P. |0 (orcid)0000-0003-4037-1554 |4 aut | |
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10.1007/s42161-021-00945-8 doi (DE-627)SPR046315128 (SPR)s42161-021-00945-8-e DE-627 ger DE-627 rakwb eng Carvalho, Mariana R. verfasserin (orcid)0000-0002-1738-0674 aut Bioassay methods for extraction and detection of antifungal potential of the epicuticular layer of Theobroma cacao pods against the witches’ broom pathogen 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2021 Abstract The witches’ broom disease of cacao, caused by the fungus Moniliophthora perniciosa, is among the main diseases of cacao (Theobroma cacao L.), one of the most important cultures in the tropics, for which chemical control is not very effective. We suggested that the epicuticular wax layer of cacao pods might have antifungal activity against Moniliophthora perniciosa. The objective of this article was to establish a protocol for extraction of the epicuticular layer of cacao pods and establish a bioassay to evaluate the in vitro antifungal activity of these epicuticular extracts on germination of M. perniciosa. First, we standardized the extraction protocol, and extracts of the epicuticular layer from standard, susceptible, and resistant cacao clones to WBDC were obtained. Then, we used an in vitro agar bioassay to evaluate the antifungal activity of the extracts against M. perniciosa basidiospores. The inhibition of the germination was highest (87.2%) for spores treated with the extracts from the resistant genotype. Treatment with extracts from ICS1 (susceptible genotype) did not significantly influence spore germination. We successfully extracted T. cacao wax and suggested that the epicuticular layer of cacao genotypes has antifungal molecules with inhibitory activity against the germination of M. perniciosa basidiospores in vitro. Cacao tree (dpeaa)DE-He213 Bioassay (dpeaa)DE-He213 Extraction protocol (dpeaa)DE-He213 Wax (dpeaa)DE-He213 Witches’ broom disease (dpeaa)DE-He213 Sena, Kaleandra F. aut Lopes, Uilson V. aut Pirovani, Carlos P. aut dos Santos Filho, Lindolfo P. aut Araújo, Saulo Andrade aut Valle, Raúl R. aut Gramacho, Karina P. (orcid)0000-0003-4037-1554 aut Enthalten in Journal of plant pathology [Cham] : Springer International Publishing, 1997 104(2021), 1 vom: 26. Okt., Seite 343-348 (DE-627)504102400 (DE-600)2212051-8 2239-7264 nnns volume:104 year:2021 number:1 day:26 month:10 pages:343-348 https://dx.doi.org/10.1007/s42161-021-00945-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 104 2021 1 26 10 343-348 |
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10.1007/s42161-021-00945-8 doi (DE-627)SPR046315128 (SPR)s42161-021-00945-8-e DE-627 ger DE-627 rakwb eng Carvalho, Mariana R. verfasserin (orcid)0000-0002-1738-0674 aut Bioassay methods for extraction and detection of antifungal potential of the epicuticular layer of Theobroma cacao pods against the witches’ broom pathogen 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2021 Abstract The witches’ broom disease of cacao, caused by the fungus Moniliophthora perniciosa, is among the main diseases of cacao (Theobroma cacao L.), one of the most important cultures in the tropics, for which chemical control is not very effective. We suggested that the epicuticular wax layer of cacao pods might have antifungal activity against Moniliophthora perniciosa. The objective of this article was to establish a protocol for extraction of the epicuticular layer of cacao pods and establish a bioassay to evaluate the in vitro antifungal activity of these epicuticular extracts on germination of M. perniciosa. First, we standardized the extraction protocol, and extracts of the epicuticular layer from standard, susceptible, and resistant cacao clones to WBDC were obtained. Then, we used an in vitro agar bioassay to evaluate the antifungal activity of the extracts against M. perniciosa basidiospores. The inhibition of the germination was highest (87.2%) for spores treated with the extracts from the resistant genotype. Treatment with extracts from ICS1 (susceptible genotype) did not significantly influence spore germination. We successfully extracted T. cacao wax and suggested that the epicuticular layer of cacao genotypes has antifungal molecules with inhibitory activity against the germination of M. perniciosa basidiospores in vitro. Cacao tree (dpeaa)DE-He213 Bioassay (dpeaa)DE-He213 Extraction protocol (dpeaa)DE-He213 Wax (dpeaa)DE-He213 Witches’ broom disease (dpeaa)DE-He213 Sena, Kaleandra F. aut Lopes, Uilson V. aut Pirovani, Carlos P. aut dos Santos Filho, Lindolfo P. aut Araújo, Saulo Andrade aut Valle, Raúl R. aut Gramacho, Karina P. (orcid)0000-0003-4037-1554 aut Enthalten in Journal of plant pathology [Cham] : Springer International Publishing, 1997 104(2021), 1 vom: 26. Okt., Seite 343-348 (DE-627)504102400 (DE-600)2212051-8 2239-7264 nnns volume:104 year:2021 number:1 day:26 month:10 pages:343-348 https://dx.doi.org/10.1007/s42161-021-00945-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 104 2021 1 26 10 343-348 |
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10.1007/s42161-021-00945-8 doi (DE-627)SPR046315128 (SPR)s42161-021-00945-8-e DE-627 ger DE-627 rakwb eng Carvalho, Mariana R. verfasserin (orcid)0000-0002-1738-0674 aut Bioassay methods for extraction and detection of antifungal potential of the epicuticular layer of Theobroma cacao pods against the witches’ broom pathogen 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2021 Abstract The witches’ broom disease of cacao, caused by the fungus Moniliophthora perniciosa, is among the main diseases of cacao (Theobroma cacao L.), one of the most important cultures in the tropics, for which chemical control is not very effective. We suggested that the epicuticular wax layer of cacao pods might have antifungal activity against Moniliophthora perniciosa. The objective of this article was to establish a protocol for extraction of the epicuticular layer of cacao pods and establish a bioassay to evaluate the in vitro antifungal activity of these epicuticular extracts on germination of M. perniciosa. First, we standardized the extraction protocol, and extracts of the epicuticular layer from standard, susceptible, and resistant cacao clones to WBDC were obtained. Then, we used an in vitro agar bioassay to evaluate the antifungal activity of the extracts against M. perniciosa basidiospores. The inhibition of the germination was highest (87.2%) for spores treated with the extracts from the resistant genotype. Treatment with extracts from ICS1 (susceptible genotype) did not significantly influence spore germination. We successfully extracted T. cacao wax and suggested that the epicuticular layer of cacao genotypes has antifungal molecules with inhibitory activity against the germination of M. perniciosa basidiospores in vitro. Cacao tree (dpeaa)DE-He213 Bioassay (dpeaa)DE-He213 Extraction protocol (dpeaa)DE-He213 Wax (dpeaa)DE-He213 Witches’ broom disease (dpeaa)DE-He213 Sena, Kaleandra F. aut Lopes, Uilson V. aut Pirovani, Carlos P. aut dos Santos Filho, Lindolfo P. aut Araújo, Saulo Andrade aut Valle, Raúl R. aut Gramacho, Karina P. (orcid)0000-0003-4037-1554 aut Enthalten in Journal of plant pathology [Cham] : Springer International Publishing, 1997 104(2021), 1 vom: 26. Okt., Seite 343-348 (DE-627)504102400 (DE-600)2212051-8 2239-7264 nnns volume:104 year:2021 number:1 day:26 month:10 pages:343-348 https://dx.doi.org/10.1007/s42161-021-00945-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 104 2021 1 26 10 343-348 |
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10.1007/s42161-021-00945-8 doi (DE-627)SPR046315128 (SPR)s42161-021-00945-8-e DE-627 ger DE-627 rakwb eng Carvalho, Mariana R. verfasserin (orcid)0000-0002-1738-0674 aut Bioassay methods for extraction and detection of antifungal potential of the epicuticular layer of Theobroma cacao pods against the witches’ broom pathogen 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2021 Abstract The witches’ broom disease of cacao, caused by the fungus Moniliophthora perniciosa, is among the main diseases of cacao (Theobroma cacao L.), one of the most important cultures in the tropics, for which chemical control is not very effective. We suggested that the epicuticular wax layer of cacao pods might have antifungal activity against Moniliophthora perniciosa. The objective of this article was to establish a protocol for extraction of the epicuticular layer of cacao pods and establish a bioassay to evaluate the in vitro antifungal activity of these epicuticular extracts on germination of M. perniciosa. First, we standardized the extraction protocol, and extracts of the epicuticular layer from standard, susceptible, and resistant cacao clones to WBDC were obtained. Then, we used an in vitro agar bioassay to evaluate the antifungal activity of the extracts against M. perniciosa basidiospores. The inhibition of the germination was highest (87.2%) for spores treated with the extracts from the resistant genotype. Treatment with extracts from ICS1 (susceptible genotype) did not significantly influence spore germination. We successfully extracted T. cacao wax and suggested that the epicuticular layer of cacao genotypes has antifungal molecules with inhibitory activity against the germination of M. perniciosa basidiospores in vitro. Cacao tree (dpeaa)DE-He213 Bioassay (dpeaa)DE-He213 Extraction protocol (dpeaa)DE-He213 Wax (dpeaa)DE-He213 Witches’ broom disease (dpeaa)DE-He213 Sena, Kaleandra F. aut Lopes, Uilson V. aut Pirovani, Carlos P. aut dos Santos Filho, Lindolfo P. aut Araújo, Saulo Andrade aut Valle, Raúl R. aut Gramacho, Karina P. (orcid)0000-0003-4037-1554 aut Enthalten in Journal of plant pathology [Cham] : Springer International Publishing, 1997 104(2021), 1 vom: 26. Okt., Seite 343-348 (DE-627)504102400 (DE-600)2212051-8 2239-7264 nnns volume:104 year:2021 number:1 day:26 month:10 pages:343-348 https://dx.doi.org/10.1007/s42161-021-00945-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 104 2021 1 26 10 343-348 |
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10.1007/s42161-021-00945-8 doi (DE-627)SPR046315128 (SPR)s42161-021-00945-8-e DE-627 ger DE-627 rakwb eng Carvalho, Mariana R. verfasserin (orcid)0000-0002-1738-0674 aut Bioassay methods for extraction and detection of antifungal potential of the epicuticular layer of Theobroma cacao pods against the witches’ broom pathogen 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2021 Abstract The witches’ broom disease of cacao, caused by the fungus Moniliophthora perniciosa, is among the main diseases of cacao (Theobroma cacao L.), one of the most important cultures in the tropics, for which chemical control is not very effective. We suggested that the epicuticular wax layer of cacao pods might have antifungal activity against Moniliophthora perniciosa. The objective of this article was to establish a protocol for extraction of the epicuticular layer of cacao pods and establish a bioassay to evaluate the in vitro antifungal activity of these epicuticular extracts on germination of M. perniciosa. First, we standardized the extraction protocol, and extracts of the epicuticular layer from standard, susceptible, and resistant cacao clones to WBDC were obtained. Then, we used an in vitro agar bioassay to evaluate the antifungal activity of the extracts against M. perniciosa basidiospores. The inhibition of the germination was highest (87.2%) for spores treated with the extracts from the resistant genotype. Treatment with extracts from ICS1 (susceptible genotype) did not significantly influence spore germination. We successfully extracted T. cacao wax and suggested that the epicuticular layer of cacao genotypes has antifungal molecules with inhibitory activity against the germination of M. perniciosa basidiospores in vitro. Cacao tree (dpeaa)DE-He213 Bioassay (dpeaa)DE-He213 Extraction protocol (dpeaa)DE-He213 Wax (dpeaa)DE-He213 Witches’ broom disease (dpeaa)DE-He213 Sena, Kaleandra F. aut Lopes, Uilson V. aut Pirovani, Carlos P. aut dos Santos Filho, Lindolfo P. aut Araújo, Saulo Andrade aut Valle, Raúl R. aut Gramacho, Karina P. (orcid)0000-0003-4037-1554 aut Enthalten in Journal of plant pathology [Cham] : Springer International Publishing, 1997 104(2021), 1 vom: 26. Okt., Seite 343-348 (DE-627)504102400 (DE-600)2212051-8 2239-7264 nnns volume:104 year:2021 number:1 day:26 month:10 pages:343-348 https://dx.doi.org/10.1007/s42161-021-00945-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 104 2021 1 26 10 343-348 |
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Enthalten in Journal of plant pathology 104(2021), 1 vom: 26. Okt., Seite 343-348 volume:104 year:2021 number:1 day:26 month:10 pages:343-348 |
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Enthalten in Journal of plant pathology 104(2021), 1 vom: 26. Okt., Seite 343-348 volume:104 year:2021 number:1 day:26 month:10 pages:343-348 |
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Cacao tree Bioassay Extraction protocol Wax Witches’ broom disease |
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Carvalho, Mariana R. @@aut@@ Sena, Kaleandra F. @@aut@@ Lopes, Uilson V. @@aut@@ Pirovani, Carlos P. @@aut@@ dos Santos Filho, Lindolfo P. @@aut@@ Araújo, Saulo Andrade @@aut@@ Valle, Raúl R. @@aut@@ Gramacho, Karina P. @@aut@@ |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR046315128</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519231939.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">220223s2021 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s42161-021-00945-8</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR046315128</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s42161-021-00945-8-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Carvalho, Mariana R.</subfield><subfield code="e">verfasserin</subfield><subfield code="0">(orcid)0000-0002-1738-0674</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Bioassay methods for extraction and detection of antifungal potential of the epicuticular layer of Theobroma cacao pods against the witches’ broom pathogen</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2021</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2021</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The witches’ broom disease of cacao, caused by the fungus Moniliophthora perniciosa, is among the main diseases of cacao (Theobroma cacao L.), one of the most important cultures in the tropics, for which chemical control is not very effective. We suggested that the epicuticular wax layer of cacao pods might have antifungal activity against Moniliophthora perniciosa. The objective of this article was to establish a protocol for extraction of the epicuticular layer of cacao pods and establish a bioassay to evaluate the in vitro antifungal activity of these epicuticular extracts on germination of M. perniciosa. First, we standardized the extraction protocol, and extracts of the epicuticular layer from standard, susceptible, and resistant cacao clones to WBDC were obtained. Then, we used an in vitro agar bioassay to evaluate the antifungal activity of the extracts against M. perniciosa basidiospores. The inhibition of the germination was highest (87.2%) for spores treated with the extracts from the resistant genotype. Treatment with extracts from ICS1 (susceptible genotype) did not significantly influence spore germination. 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|
| author |
Carvalho, Mariana R. |
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Carvalho, Mariana R. misc Cacao tree misc Bioassay misc Extraction protocol misc Wax misc Witches’ broom disease Bioassay methods for extraction and detection of antifungal potential of the epicuticular layer of Theobroma cacao pods against the witches’ broom pathogen |
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Bioassay methods for extraction and detection of antifungal potential of the epicuticular layer of Theobroma cacao pods against the witches’ broom pathogen Cacao tree (dpeaa)DE-He213 Bioassay (dpeaa)DE-He213 Extraction protocol (dpeaa)DE-He213 Wax (dpeaa)DE-He213 Witches’ broom disease (dpeaa)DE-He213 |
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Bioassay methods for extraction and detection of antifungal potential of the epicuticular layer of Theobroma cacao pods against the witches’ broom pathogen |
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Bioassay methods for extraction and detection of antifungal potential of the epicuticular layer of Theobroma cacao pods against the witches’ broom pathogen |
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Carvalho, Mariana R. Sena, Kaleandra F. Lopes, Uilson V. Pirovani, Carlos P. dos Santos Filho, Lindolfo P. Araújo, Saulo Andrade Valle, Raúl R. Gramacho, Karina P. |
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Elektronische Aufsätze |
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Carvalho, Mariana R. |
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10.1007/s42161-021-00945-8 |
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bioassay methods for extraction and detection of antifungal potential of the epicuticular layer of theobroma cacao pods against the witches’ broom pathogen |
| title_auth |
Bioassay methods for extraction and detection of antifungal potential of the epicuticular layer of Theobroma cacao pods against the witches’ broom pathogen |
| abstract |
Abstract The witches’ broom disease of cacao, caused by the fungus Moniliophthora perniciosa, is among the main diseases of cacao (Theobroma cacao L.), one of the most important cultures in the tropics, for which chemical control is not very effective. We suggested that the epicuticular wax layer of cacao pods might have antifungal activity against Moniliophthora perniciosa. The objective of this article was to establish a protocol for extraction of the epicuticular layer of cacao pods and establish a bioassay to evaluate the in vitro antifungal activity of these epicuticular extracts on germination of M. perniciosa. First, we standardized the extraction protocol, and extracts of the epicuticular layer from standard, susceptible, and resistant cacao clones to WBDC were obtained. Then, we used an in vitro agar bioassay to evaluate the antifungal activity of the extracts against M. perniciosa basidiospores. The inhibition of the germination was highest (87.2%) for spores treated with the extracts from the resistant genotype. Treatment with extracts from ICS1 (susceptible genotype) did not significantly influence spore germination. We successfully extracted T. cacao wax and suggested that the epicuticular layer of cacao genotypes has antifungal molecules with inhibitory activity against the germination of M. perniciosa basidiospores in vitro. © Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2021 |
| abstractGer |
Abstract The witches’ broom disease of cacao, caused by the fungus Moniliophthora perniciosa, is among the main diseases of cacao (Theobroma cacao L.), one of the most important cultures in the tropics, for which chemical control is not very effective. We suggested that the epicuticular wax layer of cacao pods might have antifungal activity against Moniliophthora perniciosa. The objective of this article was to establish a protocol for extraction of the epicuticular layer of cacao pods and establish a bioassay to evaluate the in vitro antifungal activity of these epicuticular extracts on germination of M. perniciosa. First, we standardized the extraction protocol, and extracts of the epicuticular layer from standard, susceptible, and resistant cacao clones to WBDC were obtained. Then, we used an in vitro agar bioassay to evaluate the antifungal activity of the extracts against M. perniciosa basidiospores. The inhibition of the germination was highest (87.2%) for spores treated with the extracts from the resistant genotype. Treatment with extracts from ICS1 (susceptible genotype) did not significantly influence spore germination. We successfully extracted T. cacao wax and suggested that the epicuticular layer of cacao genotypes has antifungal molecules with inhibitory activity against the germination of M. perniciosa basidiospores in vitro. © Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2021 |
| abstract_unstemmed |
Abstract The witches’ broom disease of cacao, caused by the fungus Moniliophthora perniciosa, is among the main diseases of cacao (Theobroma cacao L.), one of the most important cultures in the tropics, for which chemical control is not very effective. We suggested that the epicuticular wax layer of cacao pods might have antifungal activity against Moniliophthora perniciosa. The objective of this article was to establish a protocol for extraction of the epicuticular layer of cacao pods and establish a bioassay to evaluate the in vitro antifungal activity of these epicuticular extracts on germination of M. perniciosa. First, we standardized the extraction protocol, and extracts of the epicuticular layer from standard, susceptible, and resistant cacao clones to WBDC were obtained. Then, we used an in vitro agar bioassay to evaluate the antifungal activity of the extracts against M. perniciosa basidiospores. The inhibition of the germination was highest (87.2%) for spores treated with the extracts from the resistant genotype. Treatment with extracts from ICS1 (susceptible genotype) did not significantly influence spore germination. We successfully extracted T. cacao wax and suggested that the epicuticular layer of cacao genotypes has antifungal molecules with inhibitory activity against the germination of M. perniciosa basidiospores in vitro. © Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2021 |
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Bioassay methods for extraction and detection of antifungal potential of the epicuticular layer of Theobroma cacao pods against the witches’ broom pathogen |
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Sena, Kaleandra F. Lopes, Uilson V. Pirovani, Carlos P. dos Santos Filho, Lindolfo P. Araújo, Saulo Andrade Valle, Raúl R. Gramacho, Karina P. |
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| score |
7.3988447 |