Enhancing osteoblast differentiation through small molecule-incorporated engineered nanofibrous scaffold
Objective This study aimed to investigate the effect of small molecules incorporated into the engineered nanofibrous scaffold to enhance the osteoblast differentiation Materials and methods Poly-ε-caprolactone (PCL) nanofiber matrices with lithium chloride (LiCl) were fabricated using the electrospi...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Akhtar, Maria [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2021 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 |
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| Übergeordnetes Werk: |
Enthalten in: Clinical Oral Investigations - Springer-Verlag, 2001, 26(2021), 3 vom: 22. Okt., Seite 2607-2618 |
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| Übergeordnetes Werk: |
volume:26 ; year:2021 ; number:3 ; day:22 ; month:10 ; pages:2607-2618 |
| Links: |
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| DOI / URN: |
10.1007/s00784-021-04230-x |
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SPR046402047 |
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| 520 | |a Objective This study aimed to investigate the effect of small molecules incorporated into the engineered nanofibrous scaffold to enhance the osteoblast differentiation Materials and methods Poly-ε-caprolactone (PCL) nanofiber matrices with lithium chloride (LiCl) were fabricated using the electrospinning technique. Scaffolds were characterized using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX). Scaffolds were seeded with MC3T3-E1 cells and assessed using Western blots (β-catenin), alamarBlue assay (proliferation), qPCR (osteoblast differentiation), and mineralization (Alizarin Red staining). Results We observed LiCl nanofiber scaffolds induced concentration-dependent cell proliferation that correlated with an increased β-catenin expression indicating sustained Wnt signaling. Next, we examined osteoblast differentiation markers such as osteocalcin (OCN) and Runt-related transcription factor 2 (Runx2) and noted increased expression in LiCl nanofiber scaffolds. We also noted increased bone morphogenetic protein (BMP-2, 4, and 7) expressions suggesting activated Wnt can promote cures to further osteogenic differentiation. Finally, Alizarin Red staining demonstrated increased mineral deposition in LiCl-incorporated nanofiber scaffolds. Conclusions Together, these results indicated that LiCl-incorporated nanofiber scaffolds enhance osteoblast differentiation. Clinical relevance Small molecule-incorporated nanofibrous scaffolds are an innovative clinical tool for bone tissue engineering. | ||
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10.1007/s00784-021-04230-x doi (DE-627)SPR046402047 (SPR)s00784-021-04230-x-e DE-627 ger DE-627 rakwb eng Akhtar, Maria verfasserin aut Enhancing osteoblast differentiation through small molecule-incorporated engineered nanofibrous scaffold 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 Objective This study aimed to investigate the effect of small molecules incorporated into the engineered nanofibrous scaffold to enhance the osteoblast differentiation Materials and methods Poly-ε-caprolactone (PCL) nanofiber matrices with lithium chloride (LiCl) were fabricated using the electrospinning technique. Scaffolds were characterized using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX). Scaffolds were seeded with MC3T3-E1 cells and assessed using Western blots (β-catenin), alamarBlue assay (proliferation), qPCR (osteoblast differentiation), and mineralization (Alizarin Red staining). Results We observed LiCl nanofiber scaffolds induced concentration-dependent cell proliferation that correlated with an increased β-catenin expression indicating sustained Wnt signaling. Next, we examined osteoblast differentiation markers such as osteocalcin (OCN) and Runt-related transcription factor 2 (Runx2) and noted increased expression in LiCl nanofiber scaffolds. We also noted increased bone morphogenetic protein (BMP-2, 4, and 7) expressions suggesting activated Wnt can promote cures to further osteogenic differentiation. Finally, Alizarin Red staining demonstrated increased mineral deposition in LiCl-incorporated nanofiber scaffolds. Conclusions Together, these results indicated that LiCl-incorporated nanofiber scaffolds enhance osteoblast differentiation. Clinical relevance Small molecule-incorporated nanofibrous scaffolds are an innovative clinical tool for bone tissue engineering. Poly-ε-caprolactone (dpeaa)DE-He213 Electrospinning (dpeaa)DE-He213 Nanofiber (dpeaa)DE-He213 LiCl (dpeaa)DE-He213 β-Catenin (dpeaa)DE-He213 Osteoblast differentiation (dpeaa)DE-He213 Woo, Kyung Mi aut Tahir, Muhammad aut Wu, Wenhui aut Elango, Jeevithan aut Mirza, Munazza R. aut Khan, Maryam aut Shamim, Saba aut Arany, Praveen R. aut Rahman, Saeed Ur (orcid)0000-0003-2234-2274 aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 26(2021), 3 vom: 22. Okt., Seite 2607-2618 (DE-627)SPR007794231 nnns volume:26 year:2021 number:3 day:22 month:10 pages:2607-2618 https://dx.doi.org/10.1007/s00784-021-04230-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 26 2021 3 22 10 2607-2618 |
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10.1007/s00784-021-04230-x doi (DE-627)SPR046402047 (SPR)s00784-021-04230-x-e DE-627 ger DE-627 rakwb eng Akhtar, Maria verfasserin aut Enhancing osteoblast differentiation through small molecule-incorporated engineered nanofibrous scaffold 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 Objective This study aimed to investigate the effect of small molecules incorporated into the engineered nanofibrous scaffold to enhance the osteoblast differentiation Materials and methods Poly-ε-caprolactone (PCL) nanofiber matrices with lithium chloride (LiCl) were fabricated using the electrospinning technique. Scaffolds were characterized using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX). Scaffolds were seeded with MC3T3-E1 cells and assessed using Western blots (β-catenin), alamarBlue assay (proliferation), qPCR (osteoblast differentiation), and mineralization (Alizarin Red staining). Results We observed LiCl nanofiber scaffolds induced concentration-dependent cell proliferation that correlated with an increased β-catenin expression indicating sustained Wnt signaling. Next, we examined osteoblast differentiation markers such as osteocalcin (OCN) and Runt-related transcription factor 2 (Runx2) and noted increased expression in LiCl nanofiber scaffolds. We also noted increased bone morphogenetic protein (BMP-2, 4, and 7) expressions suggesting activated Wnt can promote cures to further osteogenic differentiation. Finally, Alizarin Red staining demonstrated increased mineral deposition in LiCl-incorporated nanofiber scaffolds. Conclusions Together, these results indicated that LiCl-incorporated nanofiber scaffolds enhance osteoblast differentiation. Clinical relevance Small molecule-incorporated nanofibrous scaffolds are an innovative clinical tool for bone tissue engineering. Poly-ε-caprolactone (dpeaa)DE-He213 Electrospinning (dpeaa)DE-He213 Nanofiber (dpeaa)DE-He213 LiCl (dpeaa)DE-He213 β-Catenin (dpeaa)DE-He213 Osteoblast differentiation (dpeaa)DE-He213 Woo, Kyung Mi aut Tahir, Muhammad aut Wu, Wenhui aut Elango, Jeevithan aut Mirza, Munazza R. aut Khan, Maryam aut Shamim, Saba aut Arany, Praveen R. aut Rahman, Saeed Ur (orcid)0000-0003-2234-2274 aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 26(2021), 3 vom: 22. Okt., Seite 2607-2618 (DE-627)SPR007794231 nnns volume:26 year:2021 number:3 day:22 month:10 pages:2607-2618 https://dx.doi.org/10.1007/s00784-021-04230-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 26 2021 3 22 10 2607-2618 |
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10.1007/s00784-021-04230-x doi (DE-627)SPR046402047 (SPR)s00784-021-04230-x-e DE-627 ger DE-627 rakwb eng Akhtar, Maria verfasserin aut Enhancing osteoblast differentiation through small molecule-incorporated engineered nanofibrous scaffold 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 Objective This study aimed to investigate the effect of small molecules incorporated into the engineered nanofibrous scaffold to enhance the osteoblast differentiation Materials and methods Poly-ε-caprolactone (PCL) nanofiber matrices with lithium chloride (LiCl) were fabricated using the electrospinning technique. Scaffolds were characterized using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX). Scaffolds were seeded with MC3T3-E1 cells and assessed using Western blots (β-catenin), alamarBlue assay (proliferation), qPCR (osteoblast differentiation), and mineralization (Alizarin Red staining). Results We observed LiCl nanofiber scaffolds induced concentration-dependent cell proliferation that correlated with an increased β-catenin expression indicating sustained Wnt signaling. Next, we examined osteoblast differentiation markers such as osteocalcin (OCN) and Runt-related transcription factor 2 (Runx2) and noted increased expression in LiCl nanofiber scaffolds. We also noted increased bone morphogenetic protein (BMP-2, 4, and 7) expressions suggesting activated Wnt can promote cures to further osteogenic differentiation. Finally, Alizarin Red staining demonstrated increased mineral deposition in LiCl-incorporated nanofiber scaffolds. Conclusions Together, these results indicated that LiCl-incorporated nanofiber scaffolds enhance osteoblast differentiation. Clinical relevance Small molecule-incorporated nanofibrous scaffolds are an innovative clinical tool for bone tissue engineering. Poly-ε-caprolactone (dpeaa)DE-He213 Electrospinning (dpeaa)DE-He213 Nanofiber (dpeaa)DE-He213 LiCl (dpeaa)DE-He213 β-Catenin (dpeaa)DE-He213 Osteoblast differentiation (dpeaa)DE-He213 Woo, Kyung Mi aut Tahir, Muhammad aut Wu, Wenhui aut Elango, Jeevithan aut Mirza, Munazza R. aut Khan, Maryam aut Shamim, Saba aut Arany, Praveen R. aut Rahman, Saeed Ur (orcid)0000-0003-2234-2274 aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 26(2021), 3 vom: 22. Okt., Seite 2607-2618 (DE-627)SPR007794231 nnns volume:26 year:2021 number:3 day:22 month:10 pages:2607-2618 https://dx.doi.org/10.1007/s00784-021-04230-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 26 2021 3 22 10 2607-2618 |
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10.1007/s00784-021-04230-x doi (DE-627)SPR046402047 (SPR)s00784-021-04230-x-e DE-627 ger DE-627 rakwb eng Akhtar, Maria verfasserin aut Enhancing osteoblast differentiation through small molecule-incorporated engineered nanofibrous scaffold 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 Objective This study aimed to investigate the effect of small molecules incorporated into the engineered nanofibrous scaffold to enhance the osteoblast differentiation Materials and methods Poly-ε-caprolactone (PCL) nanofiber matrices with lithium chloride (LiCl) were fabricated using the electrospinning technique. Scaffolds were characterized using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX). Scaffolds were seeded with MC3T3-E1 cells and assessed using Western blots (β-catenin), alamarBlue assay (proliferation), qPCR (osteoblast differentiation), and mineralization (Alizarin Red staining). Results We observed LiCl nanofiber scaffolds induced concentration-dependent cell proliferation that correlated with an increased β-catenin expression indicating sustained Wnt signaling. Next, we examined osteoblast differentiation markers such as osteocalcin (OCN) and Runt-related transcription factor 2 (Runx2) and noted increased expression in LiCl nanofiber scaffolds. We also noted increased bone morphogenetic protein (BMP-2, 4, and 7) expressions suggesting activated Wnt can promote cures to further osteogenic differentiation. Finally, Alizarin Red staining demonstrated increased mineral deposition in LiCl-incorporated nanofiber scaffolds. Conclusions Together, these results indicated that LiCl-incorporated nanofiber scaffolds enhance osteoblast differentiation. Clinical relevance Small molecule-incorporated nanofibrous scaffolds are an innovative clinical tool for bone tissue engineering. Poly-ε-caprolactone (dpeaa)DE-He213 Electrospinning (dpeaa)DE-He213 Nanofiber (dpeaa)DE-He213 LiCl (dpeaa)DE-He213 β-Catenin (dpeaa)DE-He213 Osteoblast differentiation (dpeaa)DE-He213 Woo, Kyung Mi aut Tahir, Muhammad aut Wu, Wenhui aut Elango, Jeevithan aut Mirza, Munazza R. aut Khan, Maryam aut Shamim, Saba aut Arany, Praveen R. aut Rahman, Saeed Ur (orcid)0000-0003-2234-2274 aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 26(2021), 3 vom: 22. Okt., Seite 2607-2618 (DE-627)SPR007794231 nnns volume:26 year:2021 number:3 day:22 month:10 pages:2607-2618 https://dx.doi.org/10.1007/s00784-021-04230-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 26 2021 3 22 10 2607-2618 |
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10.1007/s00784-021-04230-x doi (DE-627)SPR046402047 (SPR)s00784-021-04230-x-e DE-627 ger DE-627 rakwb eng Akhtar, Maria verfasserin aut Enhancing osteoblast differentiation through small molecule-incorporated engineered nanofibrous scaffold 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 Objective This study aimed to investigate the effect of small molecules incorporated into the engineered nanofibrous scaffold to enhance the osteoblast differentiation Materials and methods Poly-ε-caprolactone (PCL) nanofiber matrices with lithium chloride (LiCl) were fabricated using the electrospinning technique. Scaffolds were characterized using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX). Scaffolds were seeded with MC3T3-E1 cells and assessed using Western blots (β-catenin), alamarBlue assay (proliferation), qPCR (osteoblast differentiation), and mineralization (Alizarin Red staining). Results We observed LiCl nanofiber scaffolds induced concentration-dependent cell proliferation that correlated with an increased β-catenin expression indicating sustained Wnt signaling. Next, we examined osteoblast differentiation markers such as osteocalcin (OCN) and Runt-related transcription factor 2 (Runx2) and noted increased expression in LiCl nanofiber scaffolds. We also noted increased bone morphogenetic protein (BMP-2, 4, and 7) expressions suggesting activated Wnt can promote cures to further osteogenic differentiation. Finally, Alizarin Red staining demonstrated increased mineral deposition in LiCl-incorporated nanofiber scaffolds. Conclusions Together, these results indicated that LiCl-incorporated nanofiber scaffolds enhance osteoblast differentiation. Clinical relevance Small molecule-incorporated nanofibrous scaffolds are an innovative clinical tool for bone tissue engineering. Poly-ε-caprolactone (dpeaa)DE-He213 Electrospinning (dpeaa)DE-He213 Nanofiber (dpeaa)DE-He213 LiCl (dpeaa)DE-He213 β-Catenin (dpeaa)DE-He213 Osteoblast differentiation (dpeaa)DE-He213 Woo, Kyung Mi aut Tahir, Muhammad aut Wu, Wenhui aut Elango, Jeevithan aut Mirza, Munazza R. aut Khan, Maryam aut Shamim, Saba aut Arany, Praveen R. aut Rahman, Saeed Ur (orcid)0000-0003-2234-2274 aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 26(2021), 3 vom: 22. Okt., Seite 2607-2618 (DE-627)SPR007794231 nnns volume:26 year:2021 number:3 day:22 month:10 pages:2607-2618 https://dx.doi.org/10.1007/s00784-021-04230-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 26 2021 3 22 10 2607-2618 |
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Akhtar, Maria |
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Enhancing osteoblast differentiation through small molecule-incorporated engineered nanofibrous scaffold Poly-ε-caprolactone (dpeaa)DE-He213 Electrospinning (dpeaa)DE-He213 Nanofiber (dpeaa)DE-He213 LiCl (dpeaa)DE-He213 β-Catenin (dpeaa)DE-He213 Osteoblast differentiation (dpeaa)DE-He213 |
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Akhtar, Maria Woo, Kyung Mi Tahir, Muhammad Wu, Wenhui Elango, Jeevithan Mirza, Munazza R. Khan, Maryam Shamim, Saba Arany, Praveen R. Rahman, Saeed Ur |
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enhancing osteoblast differentiation through small molecule-incorporated engineered nanofibrous scaffold |
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Enhancing osteoblast differentiation through small molecule-incorporated engineered nanofibrous scaffold |
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Objective This study aimed to investigate the effect of small molecules incorporated into the engineered nanofibrous scaffold to enhance the osteoblast differentiation Materials and methods Poly-ε-caprolactone (PCL) nanofiber matrices with lithium chloride (LiCl) were fabricated using the electrospinning technique. Scaffolds were characterized using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX). Scaffolds were seeded with MC3T3-E1 cells and assessed using Western blots (β-catenin), alamarBlue assay (proliferation), qPCR (osteoblast differentiation), and mineralization (Alizarin Red staining). Results We observed LiCl nanofiber scaffolds induced concentration-dependent cell proliferation that correlated with an increased β-catenin expression indicating sustained Wnt signaling. Next, we examined osteoblast differentiation markers such as osteocalcin (OCN) and Runt-related transcription factor 2 (Runx2) and noted increased expression in LiCl nanofiber scaffolds. We also noted increased bone morphogenetic protein (BMP-2, 4, and 7) expressions suggesting activated Wnt can promote cures to further osteogenic differentiation. Finally, Alizarin Red staining demonstrated increased mineral deposition in LiCl-incorporated nanofiber scaffolds. Conclusions Together, these results indicated that LiCl-incorporated nanofiber scaffolds enhance osteoblast differentiation. Clinical relevance Small molecule-incorporated nanofibrous scaffolds are an innovative clinical tool for bone tissue engineering. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 |
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Objective This study aimed to investigate the effect of small molecules incorporated into the engineered nanofibrous scaffold to enhance the osteoblast differentiation Materials and methods Poly-ε-caprolactone (PCL) nanofiber matrices with lithium chloride (LiCl) were fabricated using the electrospinning technique. Scaffolds were characterized using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX). Scaffolds were seeded with MC3T3-E1 cells and assessed using Western blots (β-catenin), alamarBlue assay (proliferation), qPCR (osteoblast differentiation), and mineralization (Alizarin Red staining). Results We observed LiCl nanofiber scaffolds induced concentration-dependent cell proliferation that correlated with an increased β-catenin expression indicating sustained Wnt signaling. Next, we examined osteoblast differentiation markers such as osteocalcin (OCN) and Runt-related transcription factor 2 (Runx2) and noted increased expression in LiCl nanofiber scaffolds. We also noted increased bone morphogenetic protein (BMP-2, 4, and 7) expressions suggesting activated Wnt can promote cures to further osteogenic differentiation. Finally, Alizarin Red staining demonstrated increased mineral deposition in LiCl-incorporated nanofiber scaffolds. Conclusions Together, these results indicated that LiCl-incorporated nanofiber scaffolds enhance osteoblast differentiation. Clinical relevance Small molecule-incorporated nanofibrous scaffolds are an innovative clinical tool for bone tissue engineering. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 |
| abstract_unstemmed |
Objective This study aimed to investigate the effect of small molecules incorporated into the engineered nanofibrous scaffold to enhance the osteoblast differentiation Materials and methods Poly-ε-caprolactone (PCL) nanofiber matrices with lithium chloride (LiCl) were fabricated using the electrospinning technique. Scaffolds were characterized using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX). Scaffolds were seeded with MC3T3-E1 cells and assessed using Western blots (β-catenin), alamarBlue assay (proliferation), qPCR (osteoblast differentiation), and mineralization (Alizarin Red staining). Results We observed LiCl nanofiber scaffolds induced concentration-dependent cell proliferation that correlated with an increased β-catenin expression indicating sustained Wnt signaling. Next, we examined osteoblast differentiation markers such as osteocalcin (OCN) and Runt-related transcription factor 2 (Runx2) and noted increased expression in LiCl nanofiber scaffolds. We also noted increased bone morphogenetic protein (BMP-2, 4, and 7) expressions suggesting activated Wnt can promote cures to further osteogenic differentiation. Finally, Alizarin Red staining demonstrated increased mineral deposition in LiCl-incorporated nanofiber scaffolds. Conclusions Together, these results indicated that LiCl-incorporated nanofiber scaffolds enhance osteoblast differentiation. Clinical relevance Small molecule-incorporated nanofibrous scaffolds are an innovative clinical tool for bone tissue engineering. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 |
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Enhancing osteoblast differentiation through small molecule-incorporated engineered nanofibrous scaffold |
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Woo, Kyung Mi Tahir, Muhammad Wu, Wenhui Elango, Jeevithan Mirza, Munazza R. Khan, Maryam Shamim, Saba Arany, Praveen R. Rahman, Saeed Ur |
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