Lobularia maritima thioredoxin-h2 gene mitigates salt and osmotic stress damage in tobacco by modeling plant antioxidant system
Abstract Thioredoxins (Trxs) are multi-functional redox proteins characterized by a conserved redox-active CGPC site. In plants, Trxs are small antioxidant proteins encoded by a multigene family and regulate several processes during development and growth. Consequently, Thrxs are attractive candidat...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Ben Saad, Rania [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s), under exclusive licence to Springer Nature B.V. 2022 |
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| Übergeordnetes Werk: |
Enthalten in: Plant growth regulation - Dordrecht : Springer Science + Business Media B.V., 1982, 97(2022), 1 vom: 22. Feb., Seite 101-115 |
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| Übergeordnetes Werk: |
volume:97 ; year:2022 ; number:1 ; day:22 ; month:02 ; pages:101-115 |
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| DOI / URN: |
10.1007/s10725-022-00805-0 |
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| Katalog-ID: |
SPR046801103 |
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| 520 | |a Abstract Thioredoxins (Trxs) are multi-functional redox proteins characterized by a conserved redox-active CGPC site. In plants, Trxs are small antioxidant proteins encoded by a multigene family and regulate several processes during development and growth. Consequently, Thrxs are attractive candidates for transgenic plant improvement to overcome the abnormal plant growth and development observed during abiotic stress conditions. Here, we isolated the gene encoding the h-type Trx protein, LmTrxh2, from the halophyte plant Lobularia maritima and developed transgenic tobacco plants that overexpress LmTrxh2. Based on an in silico sequential, phylogenetic, and three-dimensional model analysis, LmTrxh2 protein shares high sequence identity and common structural characteristics with plant thioredoxins. RT-qPCR analysis revealed differential temporal and spatial regulation of LmTrxh2 in L. maritima exposed to salt (150 mM NaCl), osmotic (10% PEG 8000), and oxidative (10 µM $ H_{2} %$ O_{2} $) stresses. Subcellular localization using confocal microscopy revealed that LmTrxh2 localized to the plasma membrane. Transgenic LmTrxh2 tobacco lines exhibited improved salt and osmotic stress tolerance compared to non-transgenic (NT) plants, and this tolerance was associated with mitigation of oxidative damage. Finally, four stress-related genes were upregulated in LmTrxh2 transgenic tobacco plants compared to those in NT plants under control and stress conditions. These findings suggest that LmTrxh2 is an attractive candidate gene for the genetic engineering of crops with enhanced salt and osmotic stress tolerance. | ||
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10.1007/s10725-022-00805-0 doi (DE-627)SPR046801103 (SPR)s10725-022-00805-0-e DE-627 ger DE-627 rakwb eng Ben Saad, Rania verfasserin (orcid)0000-0003-0559-0911 aut Lobularia maritima thioredoxin-h2 gene mitigates salt and osmotic stress damage in tobacco by modeling plant antioxidant system 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Nature B.V. 2022 Abstract Thioredoxins (Trxs) are multi-functional redox proteins characterized by a conserved redox-active CGPC site. In plants, Trxs are small antioxidant proteins encoded by a multigene family and regulate several processes during development and growth. Consequently, Thrxs are attractive candidates for transgenic plant improvement to overcome the abnormal plant growth and development observed during abiotic stress conditions. Here, we isolated the gene encoding the h-type Trx protein, LmTrxh2, from the halophyte plant Lobularia maritima and developed transgenic tobacco plants that overexpress LmTrxh2. Based on an in silico sequential, phylogenetic, and three-dimensional model analysis, LmTrxh2 protein shares high sequence identity and common structural characteristics with plant thioredoxins. RT-qPCR analysis revealed differential temporal and spatial regulation of LmTrxh2 in L. maritima exposed to salt (150 mM NaCl), osmotic (10% PEG 8000), and oxidative (10 µM $ H_{2} %$ O_{2} $) stresses. Subcellular localization using confocal microscopy revealed that LmTrxh2 localized to the plasma membrane. Transgenic LmTrxh2 tobacco lines exhibited improved salt and osmotic stress tolerance compared to non-transgenic (NT) plants, and this tolerance was associated with mitigation of oxidative damage. Finally, four stress-related genes were upregulated in LmTrxh2 transgenic tobacco plants compared to those in NT plants under control and stress conditions. These findings suggest that LmTrxh2 is an attractive candidate gene for the genetic engineering of crops with enhanced salt and osmotic stress tolerance. gene (dpeaa)DE-He213 Salt tolerance (dpeaa)DE-He213 Osmotic tolerance (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Transgenic tobacco (dpeaa)DE-He213 Ben Romdhane, Walid aut Bouteraa, Mohamed Taieb aut Jrad, Olfa aut Ben Hsouna, Anis aut Enthalten in Plant growth regulation Dordrecht : Springer Science + Business Media B.V., 1982 97(2022), 1 vom: 22. Feb., Seite 101-115 (DE-627)270932941 (DE-600)1478400-2 1573-5087 nnns volume:97 year:2022 number:1 day:22 month:02 pages:101-115 https://dx.doi.org/10.1007/s10725-022-00805-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 97 2022 1 22 02 101-115 |
| spelling |
10.1007/s10725-022-00805-0 doi (DE-627)SPR046801103 (SPR)s10725-022-00805-0-e DE-627 ger DE-627 rakwb eng Ben Saad, Rania verfasserin (orcid)0000-0003-0559-0911 aut Lobularia maritima thioredoxin-h2 gene mitigates salt and osmotic stress damage in tobacco by modeling plant antioxidant system 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Nature B.V. 2022 Abstract Thioredoxins (Trxs) are multi-functional redox proteins characterized by a conserved redox-active CGPC site. In plants, Trxs are small antioxidant proteins encoded by a multigene family and regulate several processes during development and growth. Consequently, Thrxs are attractive candidates for transgenic plant improvement to overcome the abnormal plant growth and development observed during abiotic stress conditions. Here, we isolated the gene encoding the h-type Trx protein, LmTrxh2, from the halophyte plant Lobularia maritima and developed transgenic tobacco plants that overexpress LmTrxh2. Based on an in silico sequential, phylogenetic, and three-dimensional model analysis, LmTrxh2 protein shares high sequence identity and common structural characteristics with plant thioredoxins. RT-qPCR analysis revealed differential temporal and spatial regulation of LmTrxh2 in L. maritima exposed to salt (150 mM NaCl), osmotic (10% PEG 8000), and oxidative (10 µM $ H_{2} %$ O_{2} $) stresses. Subcellular localization using confocal microscopy revealed that LmTrxh2 localized to the plasma membrane. Transgenic LmTrxh2 tobacco lines exhibited improved salt and osmotic stress tolerance compared to non-transgenic (NT) plants, and this tolerance was associated with mitigation of oxidative damage. Finally, four stress-related genes were upregulated in LmTrxh2 transgenic tobacco plants compared to those in NT plants under control and stress conditions. These findings suggest that LmTrxh2 is an attractive candidate gene for the genetic engineering of crops with enhanced salt and osmotic stress tolerance. gene (dpeaa)DE-He213 Salt tolerance (dpeaa)DE-He213 Osmotic tolerance (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Transgenic tobacco (dpeaa)DE-He213 Ben Romdhane, Walid aut Bouteraa, Mohamed Taieb aut Jrad, Olfa aut Ben Hsouna, Anis aut Enthalten in Plant growth regulation Dordrecht : Springer Science + Business Media B.V., 1982 97(2022), 1 vom: 22. Feb., Seite 101-115 (DE-627)270932941 (DE-600)1478400-2 1573-5087 nnns volume:97 year:2022 number:1 day:22 month:02 pages:101-115 https://dx.doi.org/10.1007/s10725-022-00805-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 97 2022 1 22 02 101-115 |
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10.1007/s10725-022-00805-0 doi (DE-627)SPR046801103 (SPR)s10725-022-00805-0-e DE-627 ger DE-627 rakwb eng Ben Saad, Rania verfasserin (orcid)0000-0003-0559-0911 aut Lobularia maritima thioredoxin-h2 gene mitigates salt and osmotic stress damage in tobacco by modeling plant antioxidant system 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Nature B.V. 2022 Abstract Thioredoxins (Trxs) are multi-functional redox proteins characterized by a conserved redox-active CGPC site. In plants, Trxs are small antioxidant proteins encoded by a multigene family and regulate several processes during development and growth. Consequently, Thrxs are attractive candidates for transgenic plant improvement to overcome the abnormal plant growth and development observed during abiotic stress conditions. Here, we isolated the gene encoding the h-type Trx protein, LmTrxh2, from the halophyte plant Lobularia maritima and developed transgenic tobacco plants that overexpress LmTrxh2. Based on an in silico sequential, phylogenetic, and three-dimensional model analysis, LmTrxh2 protein shares high sequence identity and common structural characteristics with plant thioredoxins. RT-qPCR analysis revealed differential temporal and spatial regulation of LmTrxh2 in L. maritima exposed to salt (150 mM NaCl), osmotic (10% PEG 8000), and oxidative (10 µM $ H_{2} %$ O_{2} $) stresses. Subcellular localization using confocal microscopy revealed that LmTrxh2 localized to the plasma membrane. Transgenic LmTrxh2 tobacco lines exhibited improved salt and osmotic stress tolerance compared to non-transgenic (NT) plants, and this tolerance was associated with mitigation of oxidative damage. Finally, four stress-related genes were upregulated in LmTrxh2 transgenic tobacco plants compared to those in NT plants under control and stress conditions. These findings suggest that LmTrxh2 is an attractive candidate gene for the genetic engineering of crops with enhanced salt and osmotic stress tolerance. gene (dpeaa)DE-He213 Salt tolerance (dpeaa)DE-He213 Osmotic tolerance (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Transgenic tobacco (dpeaa)DE-He213 Ben Romdhane, Walid aut Bouteraa, Mohamed Taieb aut Jrad, Olfa aut Ben Hsouna, Anis aut Enthalten in Plant growth regulation Dordrecht : Springer Science + Business Media B.V., 1982 97(2022), 1 vom: 22. Feb., Seite 101-115 (DE-627)270932941 (DE-600)1478400-2 1573-5087 nnns volume:97 year:2022 number:1 day:22 month:02 pages:101-115 https://dx.doi.org/10.1007/s10725-022-00805-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 97 2022 1 22 02 101-115 |
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10.1007/s10725-022-00805-0 doi (DE-627)SPR046801103 (SPR)s10725-022-00805-0-e DE-627 ger DE-627 rakwb eng Ben Saad, Rania verfasserin (orcid)0000-0003-0559-0911 aut Lobularia maritima thioredoxin-h2 gene mitigates salt and osmotic stress damage in tobacco by modeling plant antioxidant system 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Nature B.V. 2022 Abstract Thioredoxins (Trxs) are multi-functional redox proteins characterized by a conserved redox-active CGPC site. In plants, Trxs are small antioxidant proteins encoded by a multigene family and regulate several processes during development and growth. Consequently, Thrxs are attractive candidates for transgenic plant improvement to overcome the abnormal plant growth and development observed during abiotic stress conditions. Here, we isolated the gene encoding the h-type Trx protein, LmTrxh2, from the halophyte plant Lobularia maritima and developed transgenic tobacco plants that overexpress LmTrxh2. Based on an in silico sequential, phylogenetic, and three-dimensional model analysis, LmTrxh2 protein shares high sequence identity and common structural characteristics with plant thioredoxins. RT-qPCR analysis revealed differential temporal and spatial regulation of LmTrxh2 in L. maritima exposed to salt (150 mM NaCl), osmotic (10% PEG 8000), and oxidative (10 µM $ H_{2} %$ O_{2} $) stresses. Subcellular localization using confocal microscopy revealed that LmTrxh2 localized to the plasma membrane. Transgenic LmTrxh2 tobacco lines exhibited improved salt and osmotic stress tolerance compared to non-transgenic (NT) plants, and this tolerance was associated with mitigation of oxidative damage. Finally, four stress-related genes were upregulated in LmTrxh2 transgenic tobacco plants compared to those in NT plants under control and stress conditions. These findings suggest that LmTrxh2 is an attractive candidate gene for the genetic engineering of crops with enhanced salt and osmotic stress tolerance. gene (dpeaa)DE-He213 Salt tolerance (dpeaa)DE-He213 Osmotic tolerance (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Transgenic tobacco (dpeaa)DE-He213 Ben Romdhane, Walid aut Bouteraa, Mohamed Taieb aut Jrad, Olfa aut Ben Hsouna, Anis aut Enthalten in Plant growth regulation Dordrecht : Springer Science + Business Media B.V., 1982 97(2022), 1 vom: 22. Feb., Seite 101-115 (DE-627)270932941 (DE-600)1478400-2 1573-5087 nnns volume:97 year:2022 number:1 day:22 month:02 pages:101-115 https://dx.doi.org/10.1007/s10725-022-00805-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 97 2022 1 22 02 101-115 |
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10.1007/s10725-022-00805-0 doi (DE-627)SPR046801103 (SPR)s10725-022-00805-0-e DE-627 ger DE-627 rakwb eng Ben Saad, Rania verfasserin (orcid)0000-0003-0559-0911 aut Lobularia maritima thioredoxin-h2 gene mitigates salt and osmotic stress damage in tobacco by modeling plant antioxidant system 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Nature B.V. 2022 Abstract Thioredoxins (Trxs) are multi-functional redox proteins characterized by a conserved redox-active CGPC site. In plants, Trxs are small antioxidant proteins encoded by a multigene family and regulate several processes during development and growth. Consequently, Thrxs are attractive candidates for transgenic plant improvement to overcome the abnormal plant growth and development observed during abiotic stress conditions. Here, we isolated the gene encoding the h-type Trx protein, LmTrxh2, from the halophyte plant Lobularia maritima and developed transgenic tobacco plants that overexpress LmTrxh2. Based on an in silico sequential, phylogenetic, and three-dimensional model analysis, LmTrxh2 protein shares high sequence identity and common structural characteristics with plant thioredoxins. RT-qPCR analysis revealed differential temporal and spatial regulation of LmTrxh2 in L. maritima exposed to salt (150 mM NaCl), osmotic (10% PEG 8000), and oxidative (10 µM $ H_{2} %$ O_{2} $) stresses. Subcellular localization using confocal microscopy revealed that LmTrxh2 localized to the plasma membrane. Transgenic LmTrxh2 tobacco lines exhibited improved salt and osmotic stress tolerance compared to non-transgenic (NT) plants, and this tolerance was associated with mitigation of oxidative damage. Finally, four stress-related genes were upregulated in LmTrxh2 transgenic tobacco plants compared to those in NT plants under control and stress conditions. These findings suggest that LmTrxh2 is an attractive candidate gene for the genetic engineering of crops with enhanced salt and osmotic stress tolerance. gene (dpeaa)DE-He213 Salt tolerance (dpeaa)DE-He213 Osmotic tolerance (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Transgenic tobacco (dpeaa)DE-He213 Ben Romdhane, Walid aut Bouteraa, Mohamed Taieb aut Jrad, Olfa aut Ben Hsouna, Anis aut Enthalten in Plant growth regulation Dordrecht : Springer Science + Business Media B.V., 1982 97(2022), 1 vom: 22. Feb., Seite 101-115 (DE-627)270932941 (DE-600)1478400-2 1573-5087 nnns volume:97 year:2022 number:1 day:22 month:02 pages:101-115 https://dx.doi.org/10.1007/s10725-022-00805-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 97 2022 1 22 02 101-115 |
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English |
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Enthalten in Plant growth regulation 97(2022), 1 vom: 22. Feb., Seite 101-115 volume:97 year:2022 number:1 day:22 month:02 pages:101-115 |
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Enthalten in Plant growth regulation 97(2022), 1 vom: 22. Feb., Seite 101-115 volume:97 year:2022 number:1 day:22 month:02 pages:101-115 |
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gene Salt tolerance Osmotic tolerance Oxidative stress Transgenic tobacco |
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Plant growth regulation |
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Ben Saad, Rania @@aut@@ Ben Romdhane, Walid @@aut@@ Bouteraa, Mohamed Taieb @@aut@@ Jrad, Olfa @@aut@@ Ben Hsouna, Anis @@aut@@ |
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In plants, Trxs are small antioxidant proteins encoded by a multigene family and regulate several processes during development and growth. Consequently, Thrxs are attractive candidates for transgenic plant improvement to overcome the abnormal plant growth and development observed during abiotic stress conditions. Here, we isolated the gene encoding the h-type Trx protein, LmTrxh2, from the halophyte plant Lobularia maritima and developed transgenic tobacco plants that overexpress LmTrxh2. Based on an in silico sequential, phylogenetic, and three-dimensional model analysis, LmTrxh2 protein shares high sequence identity and common structural characteristics with plant thioredoxins. RT-qPCR analysis revealed differential temporal and spatial regulation of LmTrxh2 in L. maritima exposed to salt (150 mM NaCl), osmotic (10% PEG 8000), and oxidative (10 µM $ H_{2} %$ O_{2} $) stresses. Subcellular localization using confocal microscopy revealed that LmTrxh2 localized to the plasma membrane. Transgenic LmTrxh2 tobacco lines exhibited improved salt and osmotic stress tolerance compared to non-transgenic (NT) plants, and this tolerance was associated with mitigation of oxidative damage. Finally, four stress-related genes were upregulated in LmTrxh2 transgenic tobacco plants compared to those in NT plants under control and stress conditions. 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Ben Saad, Rania |
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Ben Saad, Rania misc gene misc Salt tolerance misc Osmotic tolerance misc Oxidative stress misc Transgenic tobacco Lobularia maritima thioredoxin-h2 gene mitigates salt and osmotic stress damage in tobacco by modeling plant antioxidant system |
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Lobularia maritima thioredoxin-h2 gene mitigates salt and osmotic stress damage in tobacco by modeling plant antioxidant system gene (dpeaa)DE-He213 Salt tolerance (dpeaa)DE-He213 Osmotic tolerance (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Transgenic tobacco (dpeaa)DE-He213 |
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Lobularia maritima thioredoxin-h2 gene mitigates salt and osmotic stress damage in tobacco by modeling plant antioxidant system |
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Lobularia maritima thioredoxin-h2 gene mitigates salt and osmotic stress damage in tobacco by modeling plant antioxidant system |
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Ben Saad, Rania Ben Romdhane, Walid Bouteraa, Mohamed Taieb Jrad, Olfa Ben Hsouna, Anis |
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lobularia maritima thioredoxin-h2 gene mitigates salt and osmotic stress damage in tobacco by modeling plant antioxidant system |
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Lobularia maritima thioredoxin-h2 gene mitigates salt and osmotic stress damage in tobacco by modeling plant antioxidant system |
| abstract |
Abstract Thioredoxins (Trxs) are multi-functional redox proteins characterized by a conserved redox-active CGPC site. In plants, Trxs are small antioxidant proteins encoded by a multigene family and regulate several processes during development and growth. Consequently, Thrxs are attractive candidates for transgenic plant improvement to overcome the abnormal plant growth and development observed during abiotic stress conditions. Here, we isolated the gene encoding the h-type Trx protein, LmTrxh2, from the halophyte plant Lobularia maritima and developed transgenic tobacco plants that overexpress LmTrxh2. Based on an in silico sequential, phylogenetic, and three-dimensional model analysis, LmTrxh2 protein shares high sequence identity and common structural characteristics with plant thioredoxins. RT-qPCR analysis revealed differential temporal and spatial regulation of LmTrxh2 in L. maritima exposed to salt (150 mM NaCl), osmotic (10% PEG 8000), and oxidative (10 µM $ H_{2} %$ O_{2} $) stresses. Subcellular localization using confocal microscopy revealed that LmTrxh2 localized to the plasma membrane. Transgenic LmTrxh2 tobacco lines exhibited improved salt and osmotic stress tolerance compared to non-transgenic (NT) plants, and this tolerance was associated with mitigation of oxidative damage. Finally, four stress-related genes were upregulated in LmTrxh2 transgenic tobacco plants compared to those in NT plants under control and stress conditions. These findings suggest that LmTrxh2 is an attractive candidate gene for the genetic engineering of crops with enhanced salt and osmotic stress tolerance. © The Author(s), under exclusive licence to Springer Nature B.V. 2022 |
| abstractGer |
Abstract Thioredoxins (Trxs) are multi-functional redox proteins characterized by a conserved redox-active CGPC site. In plants, Trxs are small antioxidant proteins encoded by a multigene family and regulate several processes during development and growth. Consequently, Thrxs are attractive candidates for transgenic plant improvement to overcome the abnormal plant growth and development observed during abiotic stress conditions. Here, we isolated the gene encoding the h-type Trx protein, LmTrxh2, from the halophyte plant Lobularia maritima and developed transgenic tobacco plants that overexpress LmTrxh2. Based on an in silico sequential, phylogenetic, and three-dimensional model analysis, LmTrxh2 protein shares high sequence identity and common structural characteristics with plant thioredoxins. RT-qPCR analysis revealed differential temporal and spatial regulation of LmTrxh2 in L. maritima exposed to salt (150 mM NaCl), osmotic (10% PEG 8000), and oxidative (10 µM $ H_{2} %$ O_{2} $) stresses. Subcellular localization using confocal microscopy revealed that LmTrxh2 localized to the plasma membrane. Transgenic LmTrxh2 tobacco lines exhibited improved salt and osmotic stress tolerance compared to non-transgenic (NT) plants, and this tolerance was associated with mitigation of oxidative damage. Finally, four stress-related genes were upregulated in LmTrxh2 transgenic tobacco plants compared to those in NT plants under control and stress conditions. These findings suggest that LmTrxh2 is an attractive candidate gene for the genetic engineering of crops with enhanced salt and osmotic stress tolerance. © The Author(s), under exclusive licence to Springer Nature B.V. 2022 |
| abstract_unstemmed |
Abstract Thioredoxins (Trxs) are multi-functional redox proteins characterized by a conserved redox-active CGPC site. In plants, Trxs are small antioxidant proteins encoded by a multigene family and regulate several processes during development and growth. Consequently, Thrxs are attractive candidates for transgenic plant improvement to overcome the abnormal plant growth and development observed during abiotic stress conditions. Here, we isolated the gene encoding the h-type Trx protein, LmTrxh2, from the halophyte plant Lobularia maritima and developed transgenic tobacco plants that overexpress LmTrxh2. Based on an in silico sequential, phylogenetic, and three-dimensional model analysis, LmTrxh2 protein shares high sequence identity and common structural characteristics with plant thioredoxins. RT-qPCR analysis revealed differential temporal and spatial regulation of LmTrxh2 in L. maritima exposed to salt (150 mM NaCl), osmotic (10% PEG 8000), and oxidative (10 µM $ H_{2} %$ O_{2} $) stresses. Subcellular localization using confocal microscopy revealed that LmTrxh2 localized to the plasma membrane. Transgenic LmTrxh2 tobacco lines exhibited improved salt and osmotic stress tolerance compared to non-transgenic (NT) plants, and this tolerance was associated with mitigation of oxidative damage. Finally, four stress-related genes were upregulated in LmTrxh2 transgenic tobacco plants compared to those in NT plants under control and stress conditions. These findings suggest that LmTrxh2 is an attractive candidate gene for the genetic engineering of crops with enhanced salt and osmotic stress tolerance. © The Author(s), under exclusive licence to Springer Nature B.V. 2022 |
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Lobularia maritima thioredoxin-h2 gene mitigates salt and osmotic stress damage in tobacco by modeling plant antioxidant system |
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https://dx.doi.org/10.1007/s10725-022-00805-0 |
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Ben Romdhane, Walid Bouteraa, Mohamed Taieb Jrad, Olfa Ben Hsouna, Anis |
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| score |
7.399646 |