Rapid identification of SARS-CoV-2 in the point-of-care using digital PCR-based Dr. PCR™ Di20K COVID-19 Detection Kit without viral RNA extraction
Background Since COVID-19 was declared the pandemic by the WHO, it has continued to spread. There is a need for rapid, efficient, and accurate diagnostic kits and techniques to control its spread. Objective The diagnostic capability of the qRT-PCR-based Real-Q 2019-nCoV Detection Kit and dPCR-based...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Shin, Wonseok [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s) under exclusive licence to The Genetics Society of Korea 2022 |
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| Übergeordnetes Werk: |
Enthalten in: Genes & Genomics - The Genetics Society of Korea, 2010, 44(2022), 5 vom: 30. März, Seite 617-628 |
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| Übergeordnetes Werk: |
volume:44 ; year:2022 ; number:5 ; day:30 ; month:03 ; pages:617-628 |
| Links: |
|---|
| DOI / URN: |
10.1007/s13258-022-01242-z |
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SPR046850406 |
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| 520 | |a Background Since COVID-19 was declared the pandemic by the WHO, it has continued to spread. There is a need for rapid, efficient, and accurate diagnostic kits and techniques to control its spread. Objective The diagnostic capability of the qRT-PCR-based Real-Q 2019-nCoV Detection Kit and dPCR-based Dr. PCR™ Di20K COVID-19 Detection Kit was compared and evaluated. Methods Diagnostic tests for COVID-19 were performed using two different COVID-19 kits and 301 individual specimens with confirmed COVID-19 positive/negative at the government-accredited medical institution. Assessment of diagnostic capability was measured through diagnostic sensitivity, specificity, Cohen’s Kappa coefficient, and dilutional linearity tests. Results The COVID-19 diagnostic test results using two kits and 301 individual specimens perfectly matched the pre-diagnosis results of the medical institution. In addition, the measurement results of diagnostic sensitivity and specificity were “1”, indicating high diagnostic capability. Cohen’s Kappa coefficient value is “1”, which means that the diagnosis concordance between the two kits is “Almost Perfect”. As a result of dilutional linearity tests to evaluate their detection capability, both kits were measured with very high detection reliability. Conclusion Here, we propose that the dPCR-based Dr. PCR™ Di20K COVID-19 Detection Kit has the advantages of the dPCR method reported in the previous study and is suitable for point-of-care testing (POCT) by overcoming the limitations of space, test time, cross-over contamination, and biosafety due to omitting RNA extraction process. | ||
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10.1007/s13258-022-01242-z doi (DE-627)SPR046850406 (SPR)s13258-022-01242-z-e DE-627 ger DE-627 rakwb eng Shin, Wonseok verfasserin aut Rapid identification of SARS-CoV-2 in the point-of-care using digital PCR-based Dr. PCR™ Di20K COVID-19 Detection Kit without viral RNA extraction 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to The Genetics Society of Korea 2022 Background Since COVID-19 was declared the pandemic by the WHO, it has continued to spread. There is a need for rapid, efficient, and accurate diagnostic kits and techniques to control its spread. Objective The diagnostic capability of the qRT-PCR-based Real-Q 2019-nCoV Detection Kit and dPCR-based Dr. PCR™ Di20K COVID-19 Detection Kit was compared and evaluated. Methods Diagnostic tests for COVID-19 were performed using two different COVID-19 kits and 301 individual specimens with confirmed COVID-19 positive/negative at the government-accredited medical institution. Assessment of diagnostic capability was measured through diagnostic sensitivity, specificity, Cohen’s Kappa coefficient, and dilutional linearity tests. Results The COVID-19 diagnostic test results using two kits and 301 individual specimens perfectly matched the pre-diagnosis results of the medical institution. In addition, the measurement results of diagnostic sensitivity and specificity were “1”, indicating high diagnostic capability. Cohen’s Kappa coefficient value is “1”, which means that the diagnosis concordance between the two kits is “Almost Perfect”. As a result of dilutional linearity tests to evaluate their detection capability, both kits were measured with very high detection reliability. Conclusion Here, we propose that the dPCR-based Dr. PCR™ Di20K COVID-19 Detection Kit has the advantages of the dPCR method reported in the previous study and is suitable for point-of-care testing (POCT) by overcoming the limitations of space, test time, cross-over contamination, and biosafety due to omitting RNA extraction process. COVID-19 (dpeaa)DE-He213 qRT-PCR (dpeaa)DE-He213 dPCR (dpeaa)DE-He213 Point-of-care testing (dpeaa)DE-He213 POCT (dpeaa)DE-He213 Lee, Cherl-Joon aut Lee, Yong-Moon aut Choi, Young-Bong aut Mun, Seyoung aut Han, Kyudong (orcid)0000-0001-6791-2408 aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 44(2022), 5 vom: 30. März, Seite 617-628 (DE-627)SPR031096425 nnns volume:44 year:2022 number:5 day:30 month:03 pages:617-628 https://dx.doi.org/10.1007/s13258-022-01242-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 44 2022 5 30 03 617-628 |
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10.1007/s13258-022-01242-z doi (DE-627)SPR046850406 (SPR)s13258-022-01242-z-e DE-627 ger DE-627 rakwb eng Shin, Wonseok verfasserin aut Rapid identification of SARS-CoV-2 in the point-of-care using digital PCR-based Dr. PCR™ Di20K COVID-19 Detection Kit without viral RNA extraction 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to The Genetics Society of Korea 2022 Background Since COVID-19 was declared the pandemic by the WHO, it has continued to spread. There is a need for rapid, efficient, and accurate diagnostic kits and techniques to control its spread. Objective The diagnostic capability of the qRT-PCR-based Real-Q 2019-nCoV Detection Kit and dPCR-based Dr. PCR™ Di20K COVID-19 Detection Kit was compared and evaluated. Methods Diagnostic tests for COVID-19 were performed using two different COVID-19 kits and 301 individual specimens with confirmed COVID-19 positive/negative at the government-accredited medical institution. Assessment of diagnostic capability was measured through diagnostic sensitivity, specificity, Cohen’s Kappa coefficient, and dilutional linearity tests. Results The COVID-19 diagnostic test results using two kits and 301 individual specimens perfectly matched the pre-diagnosis results of the medical institution. In addition, the measurement results of diagnostic sensitivity and specificity were “1”, indicating high diagnostic capability. Cohen’s Kappa coefficient value is “1”, which means that the diagnosis concordance between the two kits is “Almost Perfect”. As a result of dilutional linearity tests to evaluate their detection capability, both kits were measured with very high detection reliability. Conclusion Here, we propose that the dPCR-based Dr. PCR™ Di20K COVID-19 Detection Kit has the advantages of the dPCR method reported in the previous study and is suitable for point-of-care testing (POCT) by overcoming the limitations of space, test time, cross-over contamination, and biosafety due to omitting RNA extraction process. COVID-19 (dpeaa)DE-He213 qRT-PCR (dpeaa)DE-He213 dPCR (dpeaa)DE-He213 Point-of-care testing (dpeaa)DE-He213 POCT (dpeaa)DE-He213 Lee, Cherl-Joon aut Lee, Yong-Moon aut Choi, Young-Bong aut Mun, Seyoung aut Han, Kyudong (orcid)0000-0001-6791-2408 aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 44(2022), 5 vom: 30. März, Seite 617-628 (DE-627)SPR031096425 nnns volume:44 year:2022 number:5 day:30 month:03 pages:617-628 https://dx.doi.org/10.1007/s13258-022-01242-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 44 2022 5 30 03 617-628 |
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10.1007/s13258-022-01242-z doi (DE-627)SPR046850406 (SPR)s13258-022-01242-z-e DE-627 ger DE-627 rakwb eng Shin, Wonseok verfasserin aut Rapid identification of SARS-CoV-2 in the point-of-care using digital PCR-based Dr. PCR™ Di20K COVID-19 Detection Kit without viral RNA extraction 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to The Genetics Society of Korea 2022 Background Since COVID-19 was declared the pandemic by the WHO, it has continued to spread. There is a need for rapid, efficient, and accurate diagnostic kits and techniques to control its spread. Objective The diagnostic capability of the qRT-PCR-based Real-Q 2019-nCoV Detection Kit and dPCR-based Dr. PCR™ Di20K COVID-19 Detection Kit was compared and evaluated. Methods Diagnostic tests for COVID-19 were performed using two different COVID-19 kits and 301 individual specimens with confirmed COVID-19 positive/negative at the government-accredited medical institution. Assessment of diagnostic capability was measured through diagnostic sensitivity, specificity, Cohen’s Kappa coefficient, and dilutional linearity tests. Results The COVID-19 diagnostic test results using two kits and 301 individual specimens perfectly matched the pre-diagnosis results of the medical institution. In addition, the measurement results of diagnostic sensitivity and specificity were “1”, indicating high diagnostic capability. Cohen’s Kappa coefficient value is “1”, which means that the diagnosis concordance between the two kits is “Almost Perfect”. As a result of dilutional linearity tests to evaluate their detection capability, both kits were measured with very high detection reliability. Conclusion Here, we propose that the dPCR-based Dr. PCR™ Di20K COVID-19 Detection Kit has the advantages of the dPCR method reported in the previous study and is suitable for point-of-care testing (POCT) by overcoming the limitations of space, test time, cross-over contamination, and biosafety due to omitting RNA extraction process. COVID-19 (dpeaa)DE-He213 qRT-PCR (dpeaa)DE-He213 dPCR (dpeaa)DE-He213 Point-of-care testing (dpeaa)DE-He213 POCT (dpeaa)DE-He213 Lee, Cherl-Joon aut Lee, Yong-Moon aut Choi, Young-Bong aut Mun, Seyoung aut Han, Kyudong (orcid)0000-0001-6791-2408 aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 44(2022), 5 vom: 30. März, Seite 617-628 (DE-627)SPR031096425 nnns volume:44 year:2022 number:5 day:30 month:03 pages:617-628 https://dx.doi.org/10.1007/s13258-022-01242-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 44 2022 5 30 03 617-628 |
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10.1007/s13258-022-01242-z doi (DE-627)SPR046850406 (SPR)s13258-022-01242-z-e DE-627 ger DE-627 rakwb eng Shin, Wonseok verfasserin aut Rapid identification of SARS-CoV-2 in the point-of-care using digital PCR-based Dr. PCR™ Di20K COVID-19 Detection Kit without viral RNA extraction 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to The Genetics Society of Korea 2022 Background Since COVID-19 was declared the pandemic by the WHO, it has continued to spread. There is a need for rapid, efficient, and accurate diagnostic kits and techniques to control its spread. Objective The diagnostic capability of the qRT-PCR-based Real-Q 2019-nCoV Detection Kit and dPCR-based Dr. PCR™ Di20K COVID-19 Detection Kit was compared and evaluated. Methods Diagnostic tests for COVID-19 were performed using two different COVID-19 kits and 301 individual specimens with confirmed COVID-19 positive/negative at the government-accredited medical institution. Assessment of diagnostic capability was measured through diagnostic sensitivity, specificity, Cohen’s Kappa coefficient, and dilutional linearity tests. Results The COVID-19 diagnostic test results using two kits and 301 individual specimens perfectly matched the pre-diagnosis results of the medical institution. In addition, the measurement results of diagnostic sensitivity and specificity were “1”, indicating high diagnostic capability. Cohen’s Kappa coefficient value is “1”, which means that the diagnosis concordance between the two kits is “Almost Perfect”. As a result of dilutional linearity tests to evaluate their detection capability, both kits were measured with very high detection reliability. Conclusion Here, we propose that the dPCR-based Dr. PCR™ Di20K COVID-19 Detection Kit has the advantages of the dPCR method reported in the previous study and is suitable for point-of-care testing (POCT) by overcoming the limitations of space, test time, cross-over contamination, and biosafety due to omitting RNA extraction process. COVID-19 (dpeaa)DE-He213 qRT-PCR (dpeaa)DE-He213 dPCR (dpeaa)DE-He213 Point-of-care testing (dpeaa)DE-He213 POCT (dpeaa)DE-He213 Lee, Cherl-Joon aut Lee, Yong-Moon aut Choi, Young-Bong aut Mun, Seyoung aut Han, Kyudong (orcid)0000-0001-6791-2408 aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 44(2022), 5 vom: 30. März, Seite 617-628 (DE-627)SPR031096425 nnns volume:44 year:2022 number:5 day:30 month:03 pages:617-628 https://dx.doi.org/10.1007/s13258-022-01242-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 44 2022 5 30 03 617-628 |
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10.1007/s13258-022-01242-z doi (DE-627)SPR046850406 (SPR)s13258-022-01242-z-e DE-627 ger DE-627 rakwb eng Shin, Wonseok verfasserin aut Rapid identification of SARS-CoV-2 in the point-of-care using digital PCR-based Dr. PCR™ Di20K COVID-19 Detection Kit without viral RNA extraction 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to The Genetics Society of Korea 2022 Background Since COVID-19 was declared the pandemic by the WHO, it has continued to spread. There is a need for rapid, efficient, and accurate diagnostic kits and techniques to control its spread. Objective The diagnostic capability of the qRT-PCR-based Real-Q 2019-nCoV Detection Kit and dPCR-based Dr. PCR™ Di20K COVID-19 Detection Kit was compared and evaluated. Methods Diagnostic tests for COVID-19 were performed using two different COVID-19 kits and 301 individual specimens with confirmed COVID-19 positive/negative at the government-accredited medical institution. Assessment of diagnostic capability was measured through diagnostic sensitivity, specificity, Cohen’s Kappa coefficient, and dilutional linearity tests. Results The COVID-19 diagnostic test results using two kits and 301 individual specimens perfectly matched the pre-diagnosis results of the medical institution. In addition, the measurement results of diagnostic sensitivity and specificity were “1”, indicating high diagnostic capability. Cohen’s Kappa coefficient value is “1”, which means that the diagnosis concordance between the two kits is “Almost Perfect”. As a result of dilutional linearity tests to evaluate their detection capability, both kits were measured with very high detection reliability. Conclusion Here, we propose that the dPCR-based Dr. PCR™ Di20K COVID-19 Detection Kit has the advantages of the dPCR method reported in the previous study and is suitable for point-of-care testing (POCT) by overcoming the limitations of space, test time, cross-over contamination, and biosafety due to omitting RNA extraction process. COVID-19 (dpeaa)DE-He213 qRT-PCR (dpeaa)DE-He213 dPCR (dpeaa)DE-He213 Point-of-care testing (dpeaa)DE-He213 POCT (dpeaa)DE-He213 Lee, Cherl-Joon aut Lee, Yong-Moon aut Choi, Young-Bong aut Mun, Seyoung aut Han, Kyudong (orcid)0000-0001-6791-2408 aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 44(2022), 5 vom: 30. März, Seite 617-628 (DE-627)SPR031096425 nnns volume:44 year:2022 number:5 day:30 month:03 pages:617-628 https://dx.doi.org/10.1007/s13258-022-01242-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 44 2022 5 30 03 617-628 |
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Rapid identification of SARS-CoV-2 in the point-of-care using digital PCR-based Dr. PCR™ Di20K COVID-19 Detection Kit without viral RNA extraction |
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Rapid identification of SARS-CoV-2 in the point-of-care using digital PCR-based Dr. PCR™ Di20K COVID-19 Detection Kit without viral RNA extraction |
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Shin, Wonseok |
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2022 |
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Shin, Wonseok Lee, Cherl-Joon Lee, Yong-Moon Choi, Young-Bong Mun, Seyoung Han, Kyudong |
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10.1007/s13258-022-01242-z |
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rapid identification of sars-cov-2 in the point-of-care using digital pcr-based dr. pcr™ di20k covid-19 detection kit without viral rna extraction |
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Rapid identification of SARS-CoV-2 in the point-of-care using digital PCR-based Dr. PCR™ Di20K COVID-19 Detection Kit without viral RNA extraction |
| abstract |
Background Since COVID-19 was declared the pandemic by the WHO, it has continued to spread. There is a need for rapid, efficient, and accurate diagnostic kits and techniques to control its spread. Objective The diagnostic capability of the qRT-PCR-based Real-Q 2019-nCoV Detection Kit and dPCR-based Dr. PCR™ Di20K COVID-19 Detection Kit was compared and evaluated. Methods Diagnostic tests for COVID-19 were performed using two different COVID-19 kits and 301 individual specimens with confirmed COVID-19 positive/negative at the government-accredited medical institution. Assessment of diagnostic capability was measured through diagnostic sensitivity, specificity, Cohen’s Kappa coefficient, and dilutional linearity tests. Results The COVID-19 diagnostic test results using two kits and 301 individual specimens perfectly matched the pre-diagnosis results of the medical institution. In addition, the measurement results of diagnostic sensitivity and specificity were “1”, indicating high diagnostic capability. Cohen’s Kappa coefficient value is “1”, which means that the diagnosis concordance between the two kits is “Almost Perfect”. As a result of dilutional linearity tests to evaluate their detection capability, both kits were measured with very high detection reliability. Conclusion Here, we propose that the dPCR-based Dr. PCR™ Di20K COVID-19 Detection Kit has the advantages of the dPCR method reported in the previous study and is suitable for point-of-care testing (POCT) by overcoming the limitations of space, test time, cross-over contamination, and biosafety due to omitting RNA extraction process. © The Author(s) under exclusive licence to The Genetics Society of Korea 2022 |
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Background Since COVID-19 was declared the pandemic by the WHO, it has continued to spread. There is a need for rapid, efficient, and accurate diagnostic kits and techniques to control its spread. Objective The diagnostic capability of the qRT-PCR-based Real-Q 2019-nCoV Detection Kit and dPCR-based Dr. PCR™ Di20K COVID-19 Detection Kit was compared and evaluated. Methods Diagnostic tests for COVID-19 were performed using two different COVID-19 kits and 301 individual specimens with confirmed COVID-19 positive/negative at the government-accredited medical institution. Assessment of diagnostic capability was measured through diagnostic sensitivity, specificity, Cohen’s Kappa coefficient, and dilutional linearity tests. Results The COVID-19 diagnostic test results using two kits and 301 individual specimens perfectly matched the pre-diagnosis results of the medical institution. In addition, the measurement results of diagnostic sensitivity and specificity were “1”, indicating high diagnostic capability. Cohen’s Kappa coefficient value is “1”, which means that the diagnosis concordance between the two kits is “Almost Perfect”. As a result of dilutional linearity tests to evaluate their detection capability, both kits were measured with very high detection reliability. Conclusion Here, we propose that the dPCR-based Dr. PCR™ Di20K COVID-19 Detection Kit has the advantages of the dPCR method reported in the previous study and is suitable for point-of-care testing (POCT) by overcoming the limitations of space, test time, cross-over contamination, and biosafety due to omitting RNA extraction process. © The Author(s) under exclusive licence to The Genetics Society of Korea 2022 |
| abstract_unstemmed |
Background Since COVID-19 was declared the pandemic by the WHO, it has continued to spread. There is a need for rapid, efficient, and accurate diagnostic kits and techniques to control its spread. Objective The diagnostic capability of the qRT-PCR-based Real-Q 2019-nCoV Detection Kit and dPCR-based Dr. PCR™ Di20K COVID-19 Detection Kit was compared and evaluated. Methods Diagnostic tests for COVID-19 were performed using two different COVID-19 kits and 301 individual specimens with confirmed COVID-19 positive/negative at the government-accredited medical institution. Assessment of diagnostic capability was measured through diagnostic sensitivity, specificity, Cohen’s Kappa coefficient, and dilutional linearity tests. Results The COVID-19 diagnostic test results using two kits and 301 individual specimens perfectly matched the pre-diagnosis results of the medical institution. In addition, the measurement results of diagnostic sensitivity and specificity were “1”, indicating high diagnostic capability. Cohen’s Kappa coefficient value is “1”, which means that the diagnosis concordance between the two kits is “Almost Perfect”. As a result of dilutional linearity tests to evaluate their detection capability, both kits were measured with very high detection reliability. Conclusion Here, we propose that the dPCR-based Dr. PCR™ Di20K COVID-19 Detection Kit has the advantages of the dPCR method reported in the previous study and is suitable for point-of-care testing (POCT) by overcoming the limitations of space, test time, cross-over contamination, and biosafety due to omitting RNA extraction process. © The Author(s) under exclusive licence to The Genetics Society of Korea 2022 |
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Rapid identification of SARS-CoV-2 in the point-of-care using digital PCR-based Dr. PCR™ Di20K COVID-19 Detection Kit without viral RNA extraction |
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Lee, Cherl-Joon Lee, Yong-Moon Choi, Young-Bong Mun, Seyoung Han, Kyudong |
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