Sucrose Induced HMGR to Promote Ginsenoside Biosynthesis in the Growth of Wild Cultivated Ginseng (Panax ginseng)
Abstract Wild cultivated ginseng is considered to be of higher quality than field-cultivated ginseng. Many active constituents of wild cultivated ginseng, including ginsenoside, have significant effects on human health. In the development phase of wild cultivated ginseng, we investigated the regulat...
Ausführliche Beschreibung
Autor*in: |
Ma, Rui [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2022 |
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Schlagwörter: |
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Anmerkung: |
© The Author(s) under exclusive licence to Sociedad Chilena de la Ciencia del Suelo 2022 |
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Übergeordnetes Werk: |
Enthalten in: Journal of soil science and plant nutrition - [Cham] : Springer International Publishing, 2010, 22(2022), 2 vom: 04. März, Seite 2255-2265 |
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Übergeordnetes Werk: |
volume:22 ; year:2022 ; number:2 ; day:04 ; month:03 ; pages:2255-2265 |
Links: |
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DOI / URN: |
10.1007/s42729-022-00806-y |
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Katalog-ID: |
SPR047252316 |
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520 | |a Abstract Wild cultivated ginseng is considered to be of higher quality than field-cultivated ginseng. Many active constituents of wild cultivated ginseng, including ginsenoside, have significant effects on human health. In the development phase of wild cultivated ginseng, we investigated the regulatory factors and pathways of ginsenoside biosynthesis. To determine glycolysis activity, the soluble sugar content, EL activity, and acetyl-CoA content of ginseng at various growth ages were measured. To examine the potential of ginsenoside biosynthesis, the ginsenoside contents and activities of SS, HMGR, and DXR of ginseng were measured at various growth ages. Ginseng cells were treated with various concentrations of sucrose to further test the effect of sugar on ginsenoside biosynthesis. MVA and MEP are the two primary pathways for ginsenoside biosynthesis. The key enzymes HMGR and DXR were detected when the sugar content was changed. The targets and primary pathways of sucrose regulation of ginsenoside biosynthesis in ginseng cells were investigated using MVA and MEP pathway inhibitors. We observed that the glycolysis of older wild cultivated ginseng was increased over that of younger ginseng, suggesting that older ginseng might provide adequate precursors for downstream ginsenoside biosynthesis. Furthermore, the total ginsenoside content and the activities of critical enzymes were increased by the ages of wild cultivated ginseng. Ginsenoside biosynthesis and glycolysis showed a significant linear relationship (R2 = 0.9562). We also verified that sucrose may stimulate glycolysis and ginsenoside biosynthesis at the cellular level. The MVA and MEP pathways were found to contribute to 58.15% and 39.72% of ginsenoside biosynthesis, respectively. The activity of HMGR, the rate-limiting enzyme of the MVA pathway, was increased with the increase of sucrose concentration in a dose-dependent manner (R2 = 0.9579). In contrast, the activity of DXR, the rate-limiting enzyme of the MEP pathway, was unaffected by sucrose concentration (R2 = 0.5414). Our findings suggest that the MVA pathway might be the main source of ginsenoside biosynthesis in wild cultivated ginseng. Sucrose promoted the MVA pathway over the MEP pathway by activating HMGR, resulting in increased ginsenoside biosynthesis year after year. This research contributes to a better understanding of the active constituents found in wild cultivated ginseng as it matures. | ||
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700 | 1 | |a Jiang, Rui |4 aut | |
700 | 1 | |a Sun, Liwei |4 aut | |
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10.1007/s42729-022-00806-y doi (DE-627)SPR047252316 (SPR)s42729-022-00806-y-e DE-627 ger DE-627 rakwb eng Ma, Rui verfasserin aut Sucrose Induced HMGR to Promote Ginsenoside Biosynthesis in the Growth of Wild Cultivated Ginseng (Panax ginseng) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedad Chilena de la Ciencia del Suelo 2022 Abstract Wild cultivated ginseng is considered to be of higher quality than field-cultivated ginseng. Many active constituents of wild cultivated ginseng, including ginsenoside, have significant effects on human health. In the development phase of wild cultivated ginseng, we investigated the regulatory factors and pathways of ginsenoside biosynthesis. To determine glycolysis activity, the soluble sugar content, EL activity, and acetyl-CoA content of ginseng at various growth ages were measured. To examine the potential of ginsenoside biosynthesis, the ginsenoside contents and activities of SS, HMGR, and DXR of ginseng were measured at various growth ages. Ginseng cells were treated with various concentrations of sucrose to further test the effect of sugar on ginsenoside biosynthesis. MVA and MEP are the two primary pathways for ginsenoside biosynthesis. The key enzymes HMGR and DXR were detected when the sugar content was changed. The targets and primary pathways of sucrose regulation of ginsenoside biosynthesis in ginseng cells were investigated using MVA and MEP pathway inhibitors. We observed that the glycolysis of older wild cultivated ginseng was increased over that of younger ginseng, suggesting that older ginseng might provide adequate precursors for downstream ginsenoside biosynthesis. Furthermore, the total ginsenoside content and the activities of critical enzymes were increased by the ages of wild cultivated ginseng. Ginsenoside biosynthesis and glycolysis showed a significant linear relationship (R2 = 0.9562). We also verified that sucrose may stimulate glycolysis and ginsenoside biosynthesis at the cellular level. The MVA and MEP pathways were found to contribute to 58.15% and 39.72% of ginsenoside biosynthesis, respectively. The activity of HMGR, the rate-limiting enzyme of the MVA pathway, was increased with the increase of sucrose concentration in a dose-dependent manner (R2 = 0.9579). In contrast, the activity of DXR, the rate-limiting enzyme of the MEP pathway, was unaffected by sucrose concentration (R2 = 0.5414). Our findings suggest that the MVA pathway might be the main source of ginsenoside biosynthesis in wild cultivated ginseng. Sucrose promoted the MVA pathway over the MEP pathway by activating HMGR, resulting in increased ginsenoside biosynthesis year after year. This research contributes to a better understanding of the active constituents found in wild cultivated ginseng as it matures. Wild cultivated ginseng (dpeaa)DE-He213 Sucrose (dpeaa)DE-He213 HMGR (dpeaa)DE-He213 Ginsenoside biosynthesis (dpeaa)DE-He213 MVA pathway (dpeaa)DE-He213 MEP pathway (dpeaa)DE-He213 Fu, Baoyu aut Yang, Pengdi aut Teng, Xiaoyu aut Zhao, Daqing aut Jiang, Rui aut Sun, Liwei aut Enthalten in Journal of soil science and plant nutrition [Cham] : Springer International Publishing, 2010 22(2022), 2 vom: 04. März, Seite 2255-2265 (DE-627)661265102 (DE-600)2611093-3 0718-9516 nnns volume:22 year:2022 number:2 day:04 month:03 pages:2255-2265 https://dx.doi.org/10.1007/s42729-022-00806-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 22 2022 2 04 03 2255-2265 |
spelling |
10.1007/s42729-022-00806-y doi (DE-627)SPR047252316 (SPR)s42729-022-00806-y-e DE-627 ger DE-627 rakwb eng Ma, Rui verfasserin aut Sucrose Induced HMGR to Promote Ginsenoside Biosynthesis in the Growth of Wild Cultivated Ginseng (Panax ginseng) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedad Chilena de la Ciencia del Suelo 2022 Abstract Wild cultivated ginseng is considered to be of higher quality than field-cultivated ginseng. Many active constituents of wild cultivated ginseng, including ginsenoside, have significant effects on human health. In the development phase of wild cultivated ginseng, we investigated the regulatory factors and pathways of ginsenoside biosynthesis. To determine glycolysis activity, the soluble sugar content, EL activity, and acetyl-CoA content of ginseng at various growth ages were measured. To examine the potential of ginsenoside biosynthesis, the ginsenoside contents and activities of SS, HMGR, and DXR of ginseng were measured at various growth ages. Ginseng cells were treated with various concentrations of sucrose to further test the effect of sugar on ginsenoside biosynthesis. MVA and MEP are the two primary pathways for ginsenoside biosynthesis. The key enzymes HMGR and DXR were detected when the sugar content was changed. The targets and primary pathways of sucrose regulation of ginsenoside biosynthesis in ginseng cells were investigated using MVA and MEP pathway inhibitors. We observed that the glycolysis of older wild cultivated ginseng was increased over that of younger ginseng, suggesting that older ginseng might provide adequate precursors for downstream ginsenoside biosynthesis. Furthermore, the total ginsenoside content and the activities of critical enzymes were increased by the ages of wild cultivated ginseng. Ginsenoside biosynthesis and glycolysis showed a significant linear relationship (R2 = 0.9562). We also verified that sucrose may stimulate glycolysis and ginsenoside biosynthesis at the cellular level. The MVA and MEP pathways were found to contribute to 58.15% and 39.72% of ginsenoside biosynthesis, respectively. The activity of HMGR, the rate-limiting enzyme of the MVA pathway, was increased with the increase of sucrose concentration in a dose-dependent manner (R2 = 0.9579). In contrast, the activity of DXR, the rate-limiting enzyme of the MEP pathway, was unaffected by sucrose concentration (R2 = 0.5414). Our findings suggest that the MVA pathway might be the main source of ginsenoside biosynthesis in wild cultivated ginseng. Sucrose promoted the MVA pathway over the MEP pathway by activating HMGR, resulting in increased ginsenoside biosynthesis year after year. This research contributes to a better understanding of the active constituents found in wild cultivated ginseng as it matures. Wild cultivated ginseng (dpeaa)DE-He213 Sucrose (dpeaa)DE-He213 HMGR (dpeaa)DE-He213 Ginsenoside biosynthesis (dpeaa)DE-He213 MVA pathway (dpeaa)DE-He213 MEP pathway (dpeaa)DE-He213 Fu, Baoyu aut Yang, Pengdi aut Teng, Xiaoyu aut Zhao, Daqing aut Jiang, Rui aut Sun, Liwei aut Enthalten in Journal of soil science and plant nutrition [Cham] : Springer International Publishing, 2010 22(2022), 2 vom: 04. März, Seite 2255-2265 (DE-627)661265102 (DE-600)2611093-3 0718-9516 nnns volume:22 year:2022 number:2 day:04 month:03 pages:2255-2265 https://dx.doi.org/10.1007/s42729-022-00806-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 22 2022 2 04 03 2255-2265 |
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10.1007/s42729-022-00806-y doi (DE-627)SPR047252316 (SPR)s42729-022-00806-y-e DE-627 ger DE-627 rakwb eng Ma, Rui verfasserin aut Sucrose Induced HMGR to Promote Ginsenoside Biosynthesis in the Growth of Wild Cultivated Ginseng (Panax ginseng) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedad Chilena de la Ciencia del Suelo 2022 Abstract Wild cultivated ginseng is considered to be of higher quality than field-cultivated ginseng. Many active constituents of wild cultivated ginseng, including ginsenoside, have significant effects on human health. In the development phase of wild cultivated ginseng, we investigated the regulatory factors and pathways of ginsenoside biosynthesis. To determine glycolysis activity, the soluble sugar content, EL activity, and acetyl-CoA content of ginseng at various growth ages were measured. To examine the potential of ginsenoside biosynthesis, the ginsenoside contents and activities of SS, HMGR, and DXR of ginseng were measured at various growth ages. Ginseng cells were treated with various concentrations of sucrose to further test the effect of sugar on ginsenoside biosynthesis. MVA and MEP are the two primary pathways for ginsenoside biosynthesis. The key enzymes HMGR and DXR were detected when the sugar content was changed. The targets and primary pathways of sucrose regulation of ginsenoside biosynthesis in ginseng cells were investigated using MVA and MEP pathway inhibitors. We observed that the glycolysis of older wild cultivated ginseng was increased over that of younger ginseng, suggesting that older ginseng might provide adequate precursors for downstream ginsenoside biosynthesis. Furthermore, the total ginsenoside content and the activities of critical enzymes were increased by the ages of wild cultivated ginseng. Ginsenoside biosynthesis and glycolysis showed a significant linear relationship (R2 = 0.9562). We also verified that sucrose may stimulate glycolysis and ginsenoside biosynthesis at the cellular level. The MVA and MEP pathways were found to contribute to 58.15% and 39.72% of ginsenoside biosynthesis, respectively. The activity of HMGR, the rate-limiting enzyme of the MVA pathway, was increased with the increase of sucrose concentration in a dose-dependent manner (R2 = 0.9579). In contrast, the activity of DXR, the rate-limiting enzyme of the MEP pathway, was unaffected by sucrose concentration (R2 = 0.5414). Our findings suggest that the MVA pathway might be the main source of ginsenoside biosynthesis in wild cultivated ginseng. Sucrose promoted the MVA pathway over the MEP pathway by activating HMGR, resulting in increased ginsenoside biosynthesis year after year. This research contributes to a better understanding of the active constituents found in wild cultivated ginseng as it matures. Wild cultivated ginseng (dpeaa)DE-He213 Sucrose (dpeaa)DE-He213 HMGR (dpeaa)DE-He213 Ginsenoside biosynthesis (dpeaa)DE-He213 MVA pathway (dpeaa)DE-He213 MEP pathway (dpeaa)DE-He213 Fu, Baoyu aut Yang, Pengdi aut Teng, Xiaoyu aut Zhao, Daqing aut Jiang, Rui aut Sun, Liwei aut Enthalten in Journal of soil science and plant nutrition [Cham] : Springer International Publishing, 2010 22(2022), 2 vom: 04. März, Seite 2255-2265 (DE-627)661265102 (DE-600)2611093-3 0718-9516 nnns volume:22 year:2022 number:2 day:04 month:03 pages:2255-2265 https://dx.doi.org/10.1007/s42729-022-00806-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 22 2022 2 04 03 2255-2265 |
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10.1007/s42729-022-00806-y doi (DE-627)SPR047252316 (SPR)s42729-022-00806-y-e DE-627 ger DE-627 rakwb eng Ma, Rui verfasserin aut Sucrose Induced HMGR to Promote Ginsenoside Biosynthesis in the Growth of Wild Cultivated Ginseng (Panax ginseng) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedad Chilena de la Ciencia del Suelo 2022 Abstract Wild cultivated ginseng is considered to be of higher quality than field-cultivated ginseng. Many active constituents of wild cultivated ginseng, including ginsenoside, have significant effects on human health. In the development phase of wild cultivated ginseng, we investigated the regulatory factors and pathways of ginsenoside biosynthesis. To determine glycolysis activity, the soluble sugar content, EL activity, and acetyl-CoA content of ginseng at various growth ages were measured. To examine the potential of ginsenoside biosynthesis, the ginsenoside contents and activities of SS, HMGR, and DXR of ginseng were measured at various growth ages. Ginseng cells were treated with various concentrations of sucrose to further test the effect of sugar on ginsenoside biosynthesis. MVA and MEP are the two primary pathways for ginsenoside biosynthesis. The key enzymes HMGR and DXR were detected when the sugar content was changed. The targets and primary pathways of sucrose regulation of ginsenoside biosynthesis in ginseng cells were investigated using MVA and MEP pathway inhibitors. We observed that the glycolysis of older wild cultivated ginseng was increased over that of younger ginseng, suggesting that older ginseng might provide adequate precursors for downstream ginsenoside biosynthesis. Furthermore, the total ginsenoside content and the activities of critical enzymes were increased by the ages of wild cultivated ginseng. Ginsenoside biosynthesis and glycolysis showed a significant linear relationship (R2 = 0.9562). We also verified that sucrose may stimulate glycolysis and ginsenoside biosynthesis at the cellular level. The MVA and MEP pathways were found to contribute to 58.15% and 39.72% of ginsenoside biosynthesis, respectively. The activity of HMGR, the rate-limiting enzyme of the MVA pathway, was increased with the increase of sucrose concentration in a dose-dependent manner (R2 = 0.9579). In contrast, the activity of DXR, the rate-limiting enzyme of the MEP pathway, was unaffected by sucrose concentration (R2 = 0.5414). Our findings suggest that the MVA pathway might be the main source of ginsenoside biosynthesis in wild cultivated ginseng. Sucrose promoted the MVA pathway over the MEP pathway by activating HMGR, resulting in increased ginsenoside biosynthesis year after year. This research contributes to a better understanding of the active constituents found in wild cultivated ginseng as it matures. Wild cultivated ginseng (dpeaa)DE-He213 Sucrose (dpeaa)DE-He213 HMGR (dpeaa)DE-He213 Ginsenoside biosynthesis (dpeaa)DE-He213 MVA pathway (dpeaa)DE-He213 MEP pathway (dpeaa)DE-He213 Fu, Baoyu aut Yang, Pengdi aut Teng, Xiaoyu aut Zhao, Daqing aut Jiang, Rui aut Sun, Liwei aut Enthalten in Journal of soil science and plant nutrition [Cham] : Springer International Publishing, 2010 22(2022), 2 vom: 04. März, Seite 2255-2265 (DE-627)661265102 (DE-600)2611093-3 0718-9516 nnns volume:22 year:2022 number:2 day:04 month:03 pages:2255-2265 https://dx.doi.org/10.1007/s42729-022-00806-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 22 2022 2 04 03 2255-2265 |
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10.1007/s42729-022-00806-y doi (DE-627)SPR047252316 (SPR)s42729-022-00806-y-e DE-627 ger DE-627 rakwb eng Ma, Rui verfasserin aut Sucrose Induced HMGR to Promote Ginsenoside Biosynthesis in the Growth of Wild Cultivated Ginseng (Panax ginseng) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedad Chilena de la Ciencia del Suelo 2022 Abstract Wild cultivated ginseng is considered to be of higher quality than field-cultivated ginseng. Many active constituents of wild cultivated ginseng, including ginsenoside, have significant effects on human health. In the development phase of wild cultivated ginseng, we investigated the regulatory factors and pathways of ginsenoside biosynthesis. To determine glycolysis activity, the soluble sugar content, EL activity, and acetyl-CoA content of ginseng at various growth ages were measured. To examine the potential of ginsenoside biosynthesis, the ginsenoside contents and activities of SS, HMGR, and DXR of ginseng were measured at various growth ages. Ginseng cells were treated with various concentrations of sucrose to further test the effect of sugar on ginsenoside biosynthesis. MVA and MEP are the two primary pathways for ginsenoside biosynthesis. The key enzymes HMGR and DXR were detected when the sugar content was changed. The targets and primary pathways of sucrose regulation of ginsenoside biosynthesis in ginseng cells were investigated using MVA and MEP pathway inhibitors. We observed that the glycolysis of older wild cultivated ginseng was increased over that of younger ginseng, suggesting that older ginseng might provide adequate precursors for downstream ginsenoside biosynthesis. Furthermore, the total ginsenoside content and the activities of critical enzymes were increased by the ages of wild cultivated ginseng. Ginsenoside biosynthesis and glycolysis showed a significant linear relationship (R2 = 0.9562). We also verified that sucrose may stimulate glycolysis and ginsenoside biosynthesis at the cellular level. The MVA and MEP pathways were found to contribute to 58.15% and 39.72% of ginsenoside biosynthesis, respectively. The activity of HMGR, the rate-limiting enzyme of the MVA pathway, was increased with the increase of sucrose concentration in a dose-dependent manner (R2 = 0.9579). In contrast, the activity of DXR, the rate-limiting enzyme of the MEP pathway, was unaffected by sucrose concentration (R2 = 0.5414). Our findings suggest that the MVA pathway might be the main source of ginsenoside biosynthesis in wild cultivated ginseng. Sucrose promoted the MVA pathway over the MEP pathway by activating HMGR, resulting in increased ginsenoside biosynthesis year after year. This research contributes to a better understanding of the active constituents found in wild cultivated ginseng as it matures. Wild cultivated ginseng (dpeaa)DE-He213 Sucrose (dpeaa)DE-He213 HMGR (dpeaa)DE-He213 Ginsenoside biosynthesis (dpeaa)DE-He213 MVA pathway (dpeaa)DE-He213 MEP pathway (dpeaa)DE-He213 Fu, Baoyu aut Yang, Pengdi aut Teng, Xiaoyu aut Zhao, Daqing aut Jiang, Rui aut Sun, Liwei aut Enthalten in Journal of soil science and plant nutrition [Cham] : Springer International Publishing, 2010 22(2022), 2 vom: 04. März, Seite 2255-2265 (DE-627)661265102 (DE-600)2611093-3 0718-9516 nnns volume:22 year:2022 number:2 day:04 month:03 pages:2255-2265 https://dx.doi.org/10.1007/s42729-022-00806-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 22 2022 2 04 03 2255-2265 |
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Enthalten in Journal of soil science and plant nutrition 22(2022), 2 vom: 04. März, Seite 2255-2265 volume:22 year:2022 number:2 day:04 month:03 pages:2255-2265 |
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Ma, Rui @@aut@@ Fu, Baoyu @@aut@@ Yang, Pengdi @@aut@@ Teng, Xiaoyu @@aut@@ Zhao, Daqing @@aut@@ Jiang, Rui @@aut@@ Sun, Liwei @@aut@@ |
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|
author |
Ma, Rui |
spellingShingle |
Ma, Rui misc Wild cultivated ginseng misc Sucrose misc HMGR misc Ginsenoside biosynthesis misc MVA pathway misc MEP pathway Sucrose Induced HMGR to Promote Ginsenoside Biosynthesis in the Growth of Wild Cultivated Ginseng (Panax ginseng) |
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Sucrose Induced HMGR to Promote Ginsenoside Biosynthesis in the Growth of Wild Cultivated Ginseng (Panax ginseng) Wild cultivated ginseng (dpeaa)DE-He213 Sucrose (dpeaa)DE-He213 HMGR (dpeaa)DE-He213 Ginsenoside biosynthesis (dpeaa)DE-He213 MVA pathway (dpeaa)DE-He213 MEP pathway (dpeaa)DE-He213 |
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Sucrose Induced HMGR to Promote Ginsenoside Biosynthesis in the Growth of Wild Cultivated Ginseng (Panax ginseng) |
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Sucrose Induced HMGR to Promote Ginsenoside Biosynthesis in the Growth of Wild Cultivated Ginseng (Panax ginseng) |
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Ma, Rui Fu, Baoyu Yang, Pengdi Teng, Xiaoyu Zhao, Daqing Jiang, Rui Sun, Liwei |
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10.1007/s42729-022-00806-y |
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sucrose induced hmgr to promote ginsenoside biosynthesis in the growth of wild cultivated ginseng (panax ginseng) |
title_auth |
Sucrose Induced HMGR to Promote Ginsenoside Biosynthesis in the Growth of Wild Cultivated Ginseng (Panax ginseng) |
abstract |
Abstract Wild cultivated ginseng is considered to be of higher quality than field-cultivated ginseng. Many active constituents of wild cultivated ginseng, including ginsenoside, have significant effects on human health. In the development phase of wild cultivated ginseng, we investigated the regulatory factors and pathways of ginsenoside biosynthesis. To determine glycolysis activity, the soluble sugar content, EL activity, and acetyl-CoA content of ginseng at various growth ages were measured. To examine the potential of ginsenoside biosynthesis, the ginsenoside contents and activities of SS, HMGR, and DXR of ginseng were measured at various growth ages. Ginseng cells were treated with various concentrations of sucrose to further test the effect of sugar on ginsenoside biosynthesis. MVA and MEP are the two primary pathways for ginsenoside biosynthesis. The key enzymes HMGR and DXR were detected when the sugar content was changed. The targets and primary pathways of sucrose regulation of ginsenoside biosynthesis in ginseng cells were investigated using MVA and MEP pathway inhibitors. We observed that the glycolysis of older wild cultivated ginseng was increased over that of younger ginseng, suggesting that older ginseng might provide adequate precursors for downstream ginsenoside biosynthesis. Furthermore, the total ginsenoside content and the activities of critical enzymes were increased by the ages of wild cultivated ginseng. Ginsenoside biosynthesis and glycolysis showed a significant linear relationship (R2 = 0.9562). We also verified that sucrose may stimulate glycolysis and ginsenoside biosynthesis at the cellular level. The MVA and MEP pathways were found to contribute to 58.15% and 39.72% of ginsenoside biosynthesis, respectively. The activity of HMGR, the rate-limiting enzyme of the MVA pathway, was increased with the increase of sucrose concentration in a dose-dependent manner (R2 = 0.9579). In contrast, the activity of DXR, the rate-limiting enzyme of the MEP pathway, was unaffected by sucrose concentration (R2 = 0.5414). Our findings suggest that the MVA pathway might be the main source of ginsenoside biosynthesis in wild cultivated ginseng. Sucrose promoted the MVA pathway over the MEP pathway by activating HMGR, resulting in increased ginsenoside biosynthesis year after year. This research contributes to a better understanding of the active constituents found in wild cultivated ginseng as it matures. © The Author(s) under exclusive licence to Sociedad Chilena de la Ciencia del Suelo 2022 |
abstractGer |
Abstract Wild cultivated ginseng is considered to be of higher quality than field-cultivated ginseng. Many active constituents of wild cultivated ginseng, including ginsenoside, have significant effects on human health. In the development phase of wild cultivated ginseng, we investigated the regulatory factors and pathways of ginsenoside biosynthesis. To determine glycolysis activity, the soluble sugar content, EL activity, and acetyl-CoA content of ginseng at various growth ages were measured. To examine the potential of ginsenoside biosynthesis, the ginsenoside contents and activities of SS, HMGR, and DXR of ginseng were measured at various growth ages. Ginseng cells were treated with various concentrations of sucrose to further test the effect of sugar on ginsenoside biosynthesis. MVA and MEP are the two primary pathways for ginsenoside biosynthesis. The key enzymes HMGR and DXR were detected when the sugar content was changed. The targets and primary pathways of sucrose regulation of ginsenoside biosynthesis in ginseng cells were investigated using MVA and MEP pathway inhibitors. We observed that the glycolysis of older wild cultivated ginseng was increased over that of younger ginseng, suggesting that older ginseng might provide adequate precursors for downstream ginsenoside biosynthesis. Furthermore, the total ginsenoside content and the activities of critical enzymes were increased by the ages of wild cultivated ginseng. Ginsenoside biosynthesis and glycolysis showed a significant linear relationship (R2 = 0.9562). We also verified that sucrose may stimulate glycolysis and ginsenoside biosynthesis at the cellular level. The MVA and MEP pathways were found to contribute to 58.15% and 39.72% of ginsenoside biosynthesis, respectively. The activity of HMGR, the rate-limiting enzyme of the MVA pathway, was increased with the increase of sucrose concentration in a dose-dependent manner (R2 = 0.9579). In contrast, the activity of DXR, the rate-limiting enzyme of the MEP pathway, was unaffected by sucrose concentration (R2 = 0.5414). Our findings suggest that the MVA pathway might be the main source of ginsenoside biosynthesis in wild cultivated ginseng. Sucrose promoted the MVA pathway over the MEP pathway by activating HMGR, resulting in increased ginsenoside biosynthesis year after year. This research contributes to a better understanding of the active constituents found in wild cultivated ginseng as it matures. © The Author(s) under exclusive licence to Sociedad Chilena de la Ciencia del Suelo 2022 |
abstract_unstemmed |
Abstract Wild cultivated ginseng is considered to be of higher quality than field-cultivated ginseng. Many active constituents of wild cultivated ginseng, including ginsenoside, have significant effects on human health. In the development phase of wild cultivated ginseng, we investigated the regulatory factors and pathways of ginsenoside biosynthesis. To determine glycolysis activity, the soluble sugar content, EL activity, and acetyl-CoA content of ginseng at various growth ages were measured. To examine the potential of ginsenoside biosynthesis, the ginsenoside contents and activities of SS, HMGR, and DXR of ginseng were measured at various growth ages. Ginseng cells were treated with various concentrations of sucrose to further test the effect of sugar on ginsenoside biosynthesis. MVA and MEP are the two primary pathways for ginsenoside biosynthesis. The key enzymes HMGR and DXR were detected when the sugar content was changed. The targets and primary pathways of sucrose regulation of ginsenoside biosynthesis in ginseng cells were investigated using MVA and MEP pathway inhibitors. We observed that the glycolysis of older wild cultivated ginseng was increased over that of younger ginseng, suggesting that older ginseng might provide adequate precursors for downstream ginsenoside biosynthesis. Furthermore, the total ginsenoside content and the activities of critical enzymes were increased by the ages of wild cultivated ginseng. Ginsenoside biosynthesis and glycolysis showed a significant linear relationship (R2 = 0.9562). We also verified that sucrose may stimulate glycolysis and ginsenoside biosynthesis at the cellular level. The MVA and MEP pathways were found to contribute to 58.15% and 39.72% of ginsenoside biosynthesis, respectively. The activity of HMGR, the rate-limiting enzyme of the MVA pathway, was increased with the increase of sucrose concentration in a dose-dependent manner (R2 = 0.9579). In contrast, the activity of DXR, the rate-limiting enzyme of the MEP pathway, was unaffected by sucrose concentration (R2 = 0.5414). Our findings suggest that the MVA pathway might be the main source of ginsenoside biosynthesis in wild cultivated ginseng. Sucrose promoted the MVA pathway over the MEP pathway by activating HMGR, resulting in increased ginsenoside biosynthesis year after year. This research contributes to a better understanding of the active constituents found in wild cultivated ginseng as it matures. © The Author(s) under exclusive licence to Sociedad Chilena de la Ciencia del Suelo 2022 |
collection_details |
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2 |
title_short |
Sucrose Induced HMGR to Promote Ginsenoside Biosynthesis in the Growth of Wild Cultivated Ginseng (Panax ginseng) |
url |
https://dx.doi.org/10.1007/s42729-022-00806-y |
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author2 |
Fu, Baoyu Yang, Pengdi Teng, Xiaoyu Zhao, Daqing Jiang, Rui Sun, Liwei |
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Fu, Baoyu Yang, Pengdi Teng, Xiaoyu Zhao, Daqing Jiang, Rui Sun, Liwei |
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doi_str |
10.1007/s42729-022-00806-y |
up_date |
2024-07-04T02:28:22.162Z |
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|
score |
7.399867 |