Analysis of Thickness and Roughness Effects of Artificial Basement Membranes on Endothelial Cell Functions
Abstract Various cells and tissues are highly organized in vivo by basement membranes (BMs) and thus promising artificial BMs (A-BMs) constructed by electrospinning and layer-by-layer (LbL) assembly have recently attracted much attention in the tissue engineering field. However, control of cell adhe...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Zeng, Jinfeng [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Anmerkung: |
© The Japan Society for Analytical Chemistry 2021 |
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| Übergeordnetes Werk: |
Enthalten in: Analytical sciences - [Cham] : Springer International Publishing, 1985, 37(2020), 3 vom: 11. Dez., Seite 491-495 |
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| Übergeordnetes Werk: |
volume:37 ; year:2020 ; number:3 ; day:11 ; month:12 ; pages:491-495 |
| Links: |
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| DOI / URN: |
10.2116/analsci.20SCP10 |
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| Katalog-ID: |
SPR047525088 |
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| 245 | 1 | 0 | |a Analysis of Thickness and Roughness Effects of Artificial Basement Membranes on Endothelial Cell Functions |
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| 520 | |a Abstract Various cells and tissues are highly organized in vivo by basement membranes (BMs) and thus promising artificial BMs (A-BMs) constructed by electrospinning and layer-by-layer (LbL) assembly have recently attracted much attention in the tissue engineering field. However, control of cell adhesion, morphology, and migration of the attached cells on the A-BMs has not been reported yet. In this study, we investigated both thickness and roughness-dependent effects of A-BMs on the functions of endothelial cells (ECs), which resulted from different assembly concentrations. The results indicated that the roughness of A-BMs increased gradually with the increase of nanofilm thickness. EC adhesion, spreading and proliferation were inhibited on thicker A-BM surfaces with larger roughness, while interendothelial junctions and the barrier effect of confluent EC monolayers on thicker A-BM surfaces were compensated by increasing seeding cell number and expanding culture time. Our study highlights the influence of LbL assembly conditions on endothelial functions, which offers a new criterion for the design of A-BMs in well-organized 3D tissues. | ||
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| 700 | 1 | |a Matsusaki, Michiya |4 aut | |
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10.2116/analsci.20SCP10 doi (DE-627)SPR047525088 (SPR)analsci.20SCP10-e DE-627 ger DE-627 rakwb eng Zeng, Jinfeng verfasserin aut Analysis of Thickness and Roughness Effects of Artificial Basement Membranes on Endothelial Cell Functions 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Japan Society for Analytical Chemistry 2021 Abstract Various cells and tissues are highly organized in vivo by basement membranes (BMs) and thus promising artificial BMs (A-BMs) constructed by electrospinning and layer-by-layer (LbL) assembly have recently attracted much attention in the tissue engineering field. However, control of cell adhesion, morphology, and migration of the attached cells on the A-BMs has not been reported yet. In this study, we investigated both thickness and roughness-dependent effects of A-BMs on the functions of endothelial cells (ECs), which resulted from different assembly concentrations. The results indicated that the roughness of A-BMs increased gradually with the increase of nanofilm thickness. EC adhesion, spreading and proliferation were inhibited on thicker A-BM surfaces with larger roughness, while interendothelial junctions and the barrier effect of confluent EC monolayers on thicker A-BM surfaces were compensated by increasing seeding cell number and expanding culture time. Our study highlights the influence of LbL assembly conditions on endothelial functions, which offers a new criterion for the design of A-BMs in well-organized 3D tissues. Artificial basement membranes (dpeaa)DE-He213 thickness (dpeaa)DE-He213 endothelial cell function (dpeaa)DE-He213 Matsusaki, Michiya aut Enthalten in Analytical sciences [Cham] : Springer International Publishing, 1985 37(2020), 3 vom: 11. Dez., Seite 491-495 (DE-627)300895925 (DE-600)1483376-1 1348-2246 nnns volume:37 year:2020 number:3 day:11 month:12 pages:491-495 https://dx.doi.org/10.2116/analsci.20SCP10 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 37 2020 3 11 12 491-495 |
| spelling |
10.2116/analsci.20SCP10 doi (DE-627)SPR047525088 (SPR)analsci.20SCP10-e DE-627 ger DE-627 rakwb eng Zeng, Jinfeng verfasserin aut Analysis of Thickness and Roughness Effects of Artificial Basement Membranes on Endothelial Cell Functions 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Japan Society for Analytical Chemistry 2021 Abstract Various cells and tissues are highly organized in vivo by basement membranes (BMs) and thus promising artificial BMs (A-BMs) constructed by electrospinning and layer-by-layer (LbL) assembly have recently attracted much attention in the tissue engineering field. However, control of cell adhesion, morphology, and migration of the attached cells on the A-BMs has not been reported yet. In this study, we investigated both thickness and roughness-dependent effects of A-BMs on the functions of endothelial cells (ECs), which resulted from different assembly concentrations. The results indicated that the roughness of A-BMs increased gradually with the increase of nanofilm thickness. EC adhesion, spreading and proliferation were inhibited on thicker A-BM surfaces with larger roughness, while interendothelial junctions and the barrier effect of confluent EC monolayers on thicker A-BM surfaces were compensated by increasing seeding cell number and expanding culture time. Our study highlights the influence of LbL assembly conditions on endothelial functions, which offers a new criterion for the design of A-BMs in well-organized 3D tissues. Artificial basement membranes (dpeaa)DE-He213 thickness (dpeaa)DE-He213 endothelial cell function (dpeaa)DE-He213 Matsusaki, Michiya aut Enthalten in Analytical sciences [Cham] : Springer International Publishing, 1985 37(2020), 3 vom: 11. Dez., Seite 491-495 (DE-627)300895925 (DE-600)1483376-1 1348-2246 nnns volume:37 year:2020 number:3 day:11 month:12 pages:491-495 https://dx.doi.org/10.2116/analsci.20SCP10 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 37 2020 3 11 12 491-495 |
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10.2116/analsci.20SCP10 doi (DE-627)SPR047525088 (SPR)analsci.20SCP10-e DE-627 ger DE-627 rakwb eng Zeng, Jinfeng verfasserin aut Analysis of Thickness and Roughness Effects of Artificial Basement Membranes on Endothelial Cell Functions 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Japan Society for Analytical Chemistry 2021 Abstract Various cells and tissues are highly organized in vivo by basement membranes (BMs) and thus promising artificial BMs (A-BMs) constructed by electrospinning and layer-by-layer (LbL) assembly have recently attracted much attention in the tissue engineering field. However, control of cell adhesion, morphology, and migration of the attached cells on the A-BMs has not been reported yet. In this study, we investigated both thickness and roughness-dependent effects of A-BMs on the functions of endothelial cells (ECs), which resulted from different assembly concentrations. The results indicated that the roughness of A-BMs increased gradually with the increase of nanofilm thickness. EC adhesion, spreading and proliferation were inhibited on thicker A-BM surfaces with larger roughness, while interendothelial junctions and the barrier effect of confluent EC monolayers on thicker A-BM surfaces were compensated by increasing seeding cell number and expanding culture time. Our study highlights the influence of LbL assembly conditions on endothelial functions, which offers a new criterion for the design of A-BMs in well-organized 3D tissues. Artificial basement membranes (dpeaa)DE-He213 thickness (dpeaa)DE-He213 endothelial cell function (dpeaa)DE-He213 Matsusaki, Michiya aut Enthalten in Analytical sciences [Cham] : Springer International Publishing, 1985 37(2020), 3 vom: 11. Dez., Seite 491-495 (DE-627)300895925 (DE-600)1483376-1 1348-2246 nnns volume:37 year:2020 number:3 day:11 month:12 pages:491-495 https://dx.doi.org/10.2116/analsci.20SCP10 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 37 2020 3 11 12 491-495 |
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10.2116/analsci.20SCP10 doi (DE-627)SPR047525088 (SPR)analsci.20SCP10-e DE-627 ger DE-627 rakwb eng Zeng, Jinfeng verfasserin aut Analysis of Thickness and Roughness Effects of Artificial Basement Membranes on Endothelial Cell Functions 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Japan Society for Analytical Chemistry 2021 Abstract Various cells and tissues are highly organized in vivo by basement membranes (BMs) and thus promising artificial BMs (A-BMs) constructed by electrospinning and layer-by-layer (LbL) assembly have recently attracted much attention in the tissue engineering field. However, control of cell adhesion, morphology, and migration of the attached cells on the A-BMs has not been reported yet. In this study, we investigated both thickness and roughness-dependent effects of A-BMs on the functions of endothelial cells (ECs), which resulted from different assembly concentrations. The results indicated that the roughness of A-BMs increased gradually with the increase of nanofilm thickness. EC adhesion, spreading and proliferation were inhibited on thicker A-BM surfaces with larger roughness, while interendothelial junctions and the barrier effect of confluent EC monolayers on thicker A-BM surfaces were compensated by increasing seeding cell number and expanding culture time. Our study highlights the influence of LbL assembly conditions on endothelial functions, which offers a new criterion for the design of A-BMs in well-organized 3D tissues. Artificial basement membranes (dpeaa)DE-He213 thickness (dpeaa)DE-He213 endothelial cell function (dpeaa)DE-He213 Matsusaki, Michiya aut Enthalten in Analytical sciences [Cham] : Springer International Publishing, 1985 37(2020), 3 vom: 11. Dez., Seite 491-495 (DE-627)300895925 (DE-600)1483376-1 1348-2246 nnns volume:37 year:2020 number:3 day:11 month:12 pages:491-495 https://dx.doi.org/10.2116/analsci.20SCP10 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 37 2020 3 11 12 491-495 |
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Analysis of Thickness and Roughness Effects of Artificial Basement Membranes on Endothelial Cell Functions Artificial basement membranes (dpeaa)DE-He213 thickness (dpeaa)DE-He213 endothelial cell function (dpeaa)DE-He213 |
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analysis of thickness and roughness effects of artificial basement membranes on endothelial cell functions |
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Analysis of Thickness and Roughness Effects of Artificial Basement Membranes on Endothelial Cell Functions |
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Abstract Various cells and tissues are highly organized in vivo by basement membranes (BMs) and thus promising artificial BMs (A-BMs) constructed by electrospinning and layer-by-layer (LbL) assembly have recently attracted much attention in the tissue engineering field. However, control of cell adhesion, morphology, and migration of the attached cells on the A-BMs has not been reported yet. In this study, we investigated both thickness and roughness-dependent effects of A-BMs on the functions of endothelial cells (ECs), which resulted from different assembly concentrations. The results indicated that the roughness of A-BMs increased gradually with the increase of nanofilm thickness. EC adhesion, spreading and proliferation were inhibited on thicker A-BM surfaces with larger roughness, while interendothelial junctions and the barrier effect of confluent EC monolayers on thicker A-BM surfaces were compensated by increasing seeding cell number and expanding culture time. Our study highlights the influence of LbL assembly conditions on endothelial functions, which offers a new criterion for the design of A-BMs in well-organized 3D tissues. © The Japan Society for Analytical Chemistry 2021 |
| abstractGer |
Abstract Various cells and tissues are highly organized in vivo by basement membranes (BMs) and thus promising artificial BMs (A-BMs) constructed by electrospinning and layer-by-layer (LbL) assembly have recently attracted much attention in the tissue engineering field. However, control of cell adhesion, morphology, and migration of the attached cells on the A-BMs has not been reported yet. In this study, we investigated both thickness and roughness-dependent effects of A-BMs on the functions of endothelial cells (ECs), which resulted from different assembly concentrations. The results indicated that the roughness of A-BMs increased gradually with the increase of nanofilm thickness. EC adhesion, spreading and proliferation were inhibited on thicker A-BM surfaces with larger roughness, while interendothelial junctions and the barrier effect of confluent EC monolayers on thicker A-BM surfaces were compensated by increasing seeding cell number and expanding culture time. Our study highlights the influence of LbL assembly conditions on endothelial functions, which offers a new criterion for the design of A-BMs in well-organized 3D tissues. © The Japan Society for Analytical Chemistry 2021 |
| abstract_unstemmed |
Abstract Various cells and tissues are highly organized in vivo by basement membranes (BMs) and thus promising artificial BMs (A-BMs) constructed by electrospinning and layer-by-layer (LbL) assembly have recently attracted much attention in the tissue engineering field. However, control of cell adhesion, morphology, and migration of the attached cells on the A-BMs has not been reported yet. In this study, we investigated both thickness and roughness-dependent effects of A-BMs on the functions of endothelial cells (ECs), which resulted from different assembly concentrations. The results indicated that the roughness of A-BMs increased gradually with the increase of nanofilm thickness. EC adhesion, spreading and proliferation were inhibited on thicker A-BM surfaces with larger roughness, while interendothelial junctions and the barrier effect of confluent EC monolayers on thicker A-BM surfaces were compensated by increasing seeding cell number and expanding culture time. Our study highlights the influence of LbL assembly conditions on endothelial functions, which offers a new criterion for the design of A-BMs in well-organized 3D tissues. © The Japan Society for Analytical Chemistry 2021 |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR047525088</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230809064824.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">220708s2020 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.2116/analsci.20SCP10</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR047525088</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)analsci.20SCP10-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Zeng, Jinfeng</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Analysis of Thickness and Roughness Effects of Artificial Basement Membranes on Endothelial Cell Functions</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2020</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© The Japan Society for Analytical Chemistry 2021</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Various cells and tissues are highly organized in vivo by basement membranes (BMs) and thus promising artificial BMs (A-BMs) constructed by electrospinning and layer-by-layer (LbL) assembly have recently attracted much attention in the tissue engineering field. However, control of cell adhesion, morphology, and migration of the attached cells on the A-BMs has not been reported yet. In this study, we investigated both thickness and roughness-dependent effects of A-BMs on the functions of endothelial cells (ECs), which resulted from different assembly concentrations. The results indicated that the roughness of A-BMs increased gradually with the increase of nanofilm thickness. EC adhesion, spreading and proliferation were inhibited on thicker A-BM surfaces with larger roughness, while interendothelial junctions and the barrier effect of confluent EC monolayers on thicker A-BM surfaces were compensated by increasing seeding cell number and expanding culture time. 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