The changing landscape of immune cells in the fetal mouse testis
Abstract Fetal testis growth involves cell influx and extensive remodeling. Immediately after sex determination in mouse, macrophages enable normal cord formation and removal of inappropriately positioned cells. This study provides new information about macrophages and other immune cells after cord...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Hosseini, Samira [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s) 2022 |
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| Übergeordnetes Werk: |
Enthalten in: Histochemistry and cell biology - Berlin : Springer, 1958, 158(2022), 4 vom: 12. Juli, Seite 345-368 |
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| Übergeordnetes Werk: |
volume:158 ; year:2022 ; number:4 ; day:12 ; month:07 ; pages:345-368 |
| Links: |
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| DOI / URN: |
10.1007/s00418-022-02129-6 |
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| Katalog-ID: |
SPR048208809 |
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| 100 | 1 | |a Hosseini, Samira |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The changing landscape of immune cells in the fetal mouse testis |
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| 520 | |a Abstract Fetal testis growth involves cell influx and extensive remodeling. Immediately after sex determination in mouse, macrophages enable normal cord formation and removal of inappropriately positioned cells. This study provides new information about macrophages and other immune cells after cord formation in fetal testes, including their density, distribution, and close cellular contacts. C57BL6J mouse testes from embryonic day (E) 13.5 to birth (post-natal day 0; PND0), were examined using immunofluorescence, immunohistochemistry, and RT-qPCR to identify macrophages (F4/80, CD206, MHCII), T cells (CD3), granulocytes/neutrophils (Ly6G), and germ cells (DDX4). F4/$ 80^{+} $ cells were the most abundant, comprising 90% of $ CD45^{+} $ cells at E13.5 and declining to 65% at PND0. Changes in size, shape, and markers (CD206 and MHCII) documented during this interval align with the understanding that F4/$ 80^{+} $ cells have different origins during embryonic life. $ CD3^{+} $ cells and F4/$ 80^{−} $/$ MHCII^{+} $ were absent to rare until PND0. $ Ly6G^{+} $ cells were scarce at E13.5 but increased robustly by PND0 to represent half of the $ CD45^{+} $ cells. These immunofluorescence data were in accord with transcript analysis, which showed that immune marker mRNAs increased with testis age. F4/$ 80^{+} $ and $ Ly6G^{+} $ cells were frequently inside cords adjacent to germ cells at E13.5 and E15.5. F4/$ 80^{+} $ cells were often in clusters next to other immune cells. Macrophages inside cords at E13.5 and E15.5 (F4/$ 80^{Hi} $/$ CD206^{+} $) were different from macrophages at PND0 (F4/$ 80^{Dim} $/$ CD206^{−} $), indicating that they have distinct origins. This histological quantification coupled with transcript information identifies new cellular interactions for immune cells in fetal testis morphogenesis, and highlights new avenues for studies of their functional significance. | ||
| 650 | 4 | |a Fetal testis development |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Macrophages |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a T cells |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Granulocytes |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Immune cell localisation |7 (dpeaa)DE-He213 | |
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| 700 | 1 | |a Moody, Sarah C. |4 aut | |
| 700 | 1 | |a Fietz, Daniela |4 aut | |
| 700 | 1 | |a Indumathy, Sivanjah |4 aut | |
| 700 | 1 | |a Schuppe, Hans-Christian |4 aut | |
| 700 | 1 | |a Hedger, Mark P. |4 aut | |
| 700 | 1 | |a Loveland, Kate L. |0 (orcid)0000-0002-5750-8046 |4 aut | |
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| 912 | |a GBV_ILN_2068 | ||
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10.1007/s00418-022-02129-6 doi (DE-627)SPR048208809 (SPR)s00418-022-02129-6-e DE-627 ger DE-627 rakwb eng Hosseini, Samira verfasserin aut The changing landscape of immune cells in the fetal mouse testis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Abstract Fetal testis growth involves cell influx and extensive remodeling. Immediately after sex determination in mouse, macrophages enable normal cord formation and removal of inappropriately positioned cells. This study provides new information about macrophages and other immune cells after cord formation in fetal testes, including their density, distribution, and close cellular contacts. C57BL6J mouse testes from embryonic day (E) 13.5 to birth (post-natal day 0; PND0), were examined using immunofluorescence, immunohistochemistry, and RT-qPCR to identify macrophages (F4/80, CD206, MHCII), T cells (CD3), granulocytes/neutrophils (Ly6G), and germ cells (DDX4). F4/$ 80^{+} $ cells were the most abundant, comprising 90% of $ CD45^{+} $ cells at E13.5 and declining to 65% at PND0. Changes in size, shape, and markers (CD206 and MHCII) documented during this interval align with the understanding that F4/$ 80^{+} $ cells have different origins during embryonic life. $ CD3^{+} $ cells and F4/$ 80^{−} $/$ MHCII^{+} $ were absent to rare until PND0. $ Ly6G^{+} $ cells were scarce at E13.5 but increased robustly by PND0 to represent half of the $ CD45^{+} $ cells. These immunofluorescence data were in accord with transcript analysis, which showed that immune marker mRNAs increased with testis age. F4/$ 80^{+} $ and $ Ly6G^{+} $ cells were frequently inside cords adjacent to germ cells at E13.5 and E15.5. F4/$ 80^{+} $ cells were often in clusters next to other immune cells. Macrophages inside cords at E13.5 and E15.5 (F4/$ 80^{Hi} $/$ CD206^{+} $) were different from macrophages at PND0 (F4/$ 80^{Dim} $/$ CD206^{−} $), indicating that they have distinct origins. This histological quantification coupled with transcript information identifies new cellular interactions for immune cells in fetal testis morphogenesis, and highlights new avenues for studies of their functional significance. Fetal testis development (dpeaa)DE-He213 Macrophages (dpeaa)DE-He213 T cells (dpeaa)DE-He213 Granulocytes (dpeaa)DE-He213 Immune cell localisation (dpeaa)DE-He213 Male germ cells (dpeaa)DE-He213 Moody, Sarah C. aut Fietz, Daniela aut Indumathy, Sivanjah aut Schuppe, Hans-Christian aut Hedger, Mark P. aut Loveland, Kate L. (orcid)0000-0002-5750-8046 aut Enthalten in Histochemistry and cell biology Berlin : Springer, 1958 158(2022), 4 vom: 12. Juli, Seite 345-368 (DE-627)235503568 (DE-600)1398345-3 1432-119X nnns volume:158 year:2022 number:4 day:12 month:07 pages:345-368 https://dx.doi.org/10.1007/s00418-022-02129-6 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 158 2022 4 12 07 345-368 |
| spelling |
10.1007/s00418-022-02129-6 doi (DE-627)SPR048208809 (SPR)s00418-022-02129-6-e DE-627 ger DE-627 rakwb eng Hosseini, Samira verfasserin aut The changing landscape of immune cells in the fetal mouse testis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Abstract Fetal testis growth involves cell influx and extensive remodeling. Immediately after sex determination in mouse, macrophages enable normal cord formation and removal of inappropriately positioned cells. This study provides new information about macrophages and other immune cells after cord formation in fetal testes, including their density, distribution, and close cellular contacts. C57BL6J mouse testes from embryonic day (E) 13.5 to birth (post-natal day 0; PND0), were examined using immunofluorescence, immunohistochemistry, and RT-qPCR to identify macrophages (F4/80, CD206, MHCII), T cells (CD3), granulocytes/neutrophils (Ly6G), and germ cells (DDX4). F4/$ 80^{+} $ cells were the most abundant, comprising 90% of $ CD45^{+} $ cells at E13.5 and declining to 65% at PND0. Changes in size, shape, and markers (CD206 and MHCII) documented during this interval align with the understanding that F4/$ 80^{+} $ cells have different origins during embryonic life. $ CD3^{+} $ cells and F4/$ 80^{−} $/$ MHCII^{+} $ were absent to rare until PND0. $ Ly6G^{+} $ cells were scarce at E13.5 but increased robustly by PND0 to represent half of the $ CD45^{+} $ cells. These immunofluorescence data were in accord with transcript analysis, which showed that immune marker mRNAs increased with testis age. F4/$ 80^{+} $ and $ Ly6G^{+} $ cells were frequently inside cords adjacent to germ cells at E13.5 and E15.5. F4/$ 80^{+} $ cells were often in clusters next to other immune cells. Macrophages inside cords at E13.5 and E15.5 (F4/$ 80^{Hi} $/$ CD206^{+} $) were different from macrophages at PND0 (F4/$ 80^{Dim} $/$ CD206^{−} $), indicating that they have distinct origins. This histological quantification coupled with transcript information identifies new cellular interactions for immune cells in fetal testis morphogenesis, and highlights new avenues for studies of their functional significance. Fetal testis development (dpeaa)DE-He213 Macrophages (dpeaa)DE-He213 T cells (dpeaa)DE-He213 Granulocytes (dpeaa)DE-He213 Immune cell localisation (dpeaa)DE-He213 Male germ cells (dpeaa)DE-He213 Moody, Sarah C. aut Fietz, Daniela aut Indumathy, Sivanjah aut Schuppe, Hans-Christian aut Hedger, Mark P. aut Loveland, Kate L. (orcid)0000-0002-5750-8046 aut Enthalten in Histochemistry and cell biology Berlin : Springer, 1958 158(2022), 4 vom: 12. Juli, Seite 345-368 (DE-627)235503568 (DE-600)1398345-3 1432-119X nnns volume:158 year:2022 number:4 day:12 month:07 pages:345-368 https://dx.doi.org/10.1007/s00418-022-02129-6 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 158 2022 4 12 07 345-368 |
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10.1007/s00418-022-02129-6 doi (DE-627)SPR048208809 (SPR)s00418-022-02129-6-e DE-627 ger DE-627 rakwb eng Hosseini, Samira verfasserin aut The changing landscape of immune cells in the fetal mouse testis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Abstract Fetal testis growth involves cell influx and extensive remodeling. Immediately after sex determination in mouse, macrophages enable normal cord formation and removal of inappropriately positioned cells. This study provides new information about macrophages and other immune cells after cord formation in fetal testes, including their density, distribution, and close cellular contacts. C57BL6J mouse testes from embryonic day (E) 13.5 to birth (post-natal day 0; PND0), were examined using immunofluorescence, immunohistochemistry, and RT-qPCR to identify macrophages (F4/80, CD206, MHCII), T cells (CD3), granulocytes/neutrophils (Ly6G), and germ cells (DDX4). F4/$ 80^{+} $ cells were the most abundant, comprising 90% of $ CD45^{+} $ cells at E13.5 and declining to 65% at PND0. Changes in size, shape, and markers (CD206 and MHCII) documented during this interval align with the understanding that F4/$ 80^{+} $ cells have different origins during embryonic life. $ CD3^{+} $ cells and F4/$ 80^{−} $/$ MHCII^{+} $ were absent to rare until PND0. $ Ly6G^{+} $ cells were scarce at E13.5 but increased robustly by PND0 to represent half of the $ CD45^{+} $ cells. These immunofluorescence data were in accord with transcript analysis, which showed that immune marker mRNAs increased with testis age. F4/$ 80^{+} $ and $ Ly6G^{+} $ cells were frequently inside cords adjacent to germ cells at E13.5 and E15.5. F4/$ 80^{+} $ cells were often in clusters next to other immune cells. Macrophages inside cords at E13.5 and E15.5 (F4/$ 80^{Hi} $/$ CD206^{+} $) were different from macrophages at PND0 (F4/$ 80^{Dim} $/$ CD206^{−} $), indicating that they have distinct origins. This histological quantification coupled with transcript information identifies new cellular interactions for immune cells in fetal testis morphogenesis, and highlights new avenues for studies of their functional significance. Fetal testis development (dpeaa)DE-He213 Macrophages (dpeaa)DE-He213 T cells (dpeaa)DE-He213 Granulocytes (dpeaa)DE-He213 Immune cell localisation (dpeaa)DE-He213 Male germ cells (dpeaa)DE-He213 Moody, Sarah C. aut Fietz, Daniela aut Indumathy, Sivanjah aut Schuppe, Hans-Christian aut Hedger, Mark P. aut Loveland, Kate L. (orcid)0000-0002-5750-8046 aut Enthalten in Histochemistry and cell biology Berlin : Springer, 1958 158(2022), 4 vom: 12. Juli, Seite 345-368 (DE-627)235503568 (DE-600)1398345-3 1432-119X nnns volume:158 year:2022 number:4 day:12 month:07 pages:345-368 https://dx.doi.org/10.1007/s00418-022-02129-6 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 158 2022 4 12 07 345-368 |
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10.1007/s00418-022-02129-6 doi (DE-627)SPR048208809 (SPR)s00418-022-02129-6-e DE-627 ger DE-627 rakwb eng Hosseini, Samira verfasserin aut The changing landscape of immune cells in the fetal mouse testis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Abstract Fetal testis growth involves cell influx and extensive remodeling. Immediately after sex determination in mouse, macrophages enable normal cord formation and removal of inappropriately positioned cells. This study provides new information about macrophages and other immune cells after cord formation in fetal testes, including their density, distribution, and close cellular contacts. C57BL6J mouse testes from embryonic day (E) 13.5 to birth (post-natal day 0; PND0), were examined using immunofluorescence, immunohistochemistry, and RT-qPCR to identify macrophages (F4/80, CD206, MHCII), T cells (CD3), granulocytes/neutrophils (Ly6G), and germ cells (DDX4). F4/$ 80^{+} $ cells were the most abundant, comprising 90% of $ CD45^{+} $ cells at E13.5 and declining to 65% at PND0. Changes in size, shape, and markers (CD206 and MHCII) documented during this interval align with the understanding that F4/$ 80^{+} $ cells have different origins during embryonic life. $ CD3^{+} $ cells and F4/$ 80^{−} $/$ MHCII^{+} $ were absent to rare until PND0. $ Ly6G^{+} $ cells were scarce at E13.5 but increased robustly by PND0 to represent half of the $ CD45^{+} $ cells. These immunofluorescence data were in accord with transcript analysis, which showed that immune marker mRNAs increased with testis age. F4/$ 80^{+} $ and $ Ly6G^{+} $ cells were frequently inside cords adjacent to germ cells at E13.5 and E15.5. F4/$ 80^{+} $ cells were often in clusters next to other immune cells. Macrophages inside cords at E13.5 and E15.5 (F4/$ 80^{Hi} $/$ CD206^{+} $) were different from macrophages at PND0 (F4/$ 80^{Dim} $/$ CD206^{−} $), indicating that they have distinct origins. This histological quantification coupled with transcript information identifies new cellular interactions for immune cells in fetal testis morphogenesis, and highlights new avenues for studies of their functional significance. Fetal testis development (dpeaa)DE-He213 Macrophages (dpeaa)DE-He213 T cells (dpeaa)DE-He213 Granulocytes (dpeaa)DE-He213 Immune cell localisation (dpeaa)DE-He213 Male germ cells (dpeaa)DE-He213 Moody, Sarah C. aut Fietz, Daniela aut Indumathy, Sivanjah aut Schuppe, Hans-Christian aut Hedger, Mark P. aut Loveland, Kate L. (orcid)0000-0002-5750-8046 aut Enthalten in Histochemistry and cell biology Berlin : Springer, 1958 158(2022), 4 vom: 12. Juli, Seite 345-368 (DE-627)235503568 (DE-600)1398345-3 1432-119X nnns volume:158 year:2022 number:4 day:12 month:07 pages:345-368 https://dx.doi.org/10.1007/s00418-022-02129-6 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 158 2022 4 12 07 345-368 |
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10.1007/s00418-022-02129-6 doi (DE-627)SPR048208809 (SPR)s00418-022-02129-6-e DE-627 ger DE-627 rakwb eng Hosseini, Samira verfasserin aut The changing landscape of immune cells in the fetal mouse testis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Abstract Fetal testis growth involves cell influx and extensive remodeling. Immediately after sex determination in mouse, macrophages enable normal cord formation and removal of inappropriately positioned cells. This study provides new information about macrophages and other immune cells after cord formation in fetal testes, including their density, distribution, and close cellular contacts. C57BL6J mouse testes from embryonic day (E) 13.5 to birth (post-natal day 0; PND0), were examined using immunofluorescence, immunohistochemistry, and RT-qPCR to identify macrophages (F4/80, CD206, MHCII), T cells (CD3), granulocytes/neutrophils (Ly6G), and germ cells (DDX4). F4/$ 80^{+} $ cells were the most abundant, comprising 90% of $ CD45^{+} $ cells at E13.5 and declining to 65% at PND0. Changes in size, shape, and markers (CD206 and MHCII) documented during this interval align with the understanding that F4/$ 80^{+} $ cells have different origins during embryonic life. $ CD3^{+} $ cells and F4/$ 80^{−} $/$ MHCII^{+} $ were absent to rare until PND0. $ Ly6G^{+} $ cells were scarce at E13.5 but increased robustly by PND0 to represent half of the $ CD45^{+} $ cells. These immunofluorescence data were in accord with transcript analysis, which showed that immune marker mRNAs increased with testis age. F4/$ 80^{+} $ and $ Ly6G^{+} $ cells were frequently inside cords adjacent to germ cells at E13.5 and E15.5. F4/$ 80^{+} $ cells were often in clusters next to other immune cells. Macrophages inside cords at E13.5 and E15.5 (F4/$ 80^{Hi} $/$ CD206^{+} $) were different from macrophages at PND0 (F4/$ 80^{Dim} $/$ CD206^{−} $), indicating that they have distinct origins. This histological quantification coupled with transcript information identifies new cellular interactions for immune cells in fetal testis morphogenesis, and highlights new avenues for studies of their functional significance. Fetal testis development (dpeaa)DE-He213 Macrophages (dpeaa)DE-He213 T cells (dpeaa)DE-He213 Granulocytes (dpeaa)DE-He213 Immune cell localisation (dpeaa)DE-He213 Male germ cells (dpeaa)DE-He213 Moody, Sarah C. aut Fietz, Daniela aut Indumathy, Sivanjah aut Schuppe, Hans-Christian aut Hedger, Mark P. aut Loveland, Kate L. (orcid)0000-0002-5750-8046 aut Enthalten in Histochemistry and cell biology Berlin : Springer, 1958 158(2022), 4 vom: 12. Juli, Seite 345-368 (DE-627)235503568 (DE-600)1398345-3 1432-119X nnns volume:158 year:2022 number:4 day:12 month:07 pages:345-368 https://dx.doi.org/10.1007/s00418-022-02129-6 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 158 2022 4 12 07 345-368 |
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Enthalten in Histochemistry and cell biology 158(2022), 4 vom: 12. Juli, Seite 345-368 volume:158 year:2022 number:4 day:12 month:07 pages:345-368 |
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Enthalten in Histochemistry and cell biology 158(2022), 4 vom: 12. Juli, Seite 345-368 volume:158 year:2022 number:4 day:12 month:07 pages:345-368 |
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Fetal testis development Macrophages T cells Granulocytes Immune cell localisation Male germ cells |
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Histochemistry and cell biology |
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Hosseini, Samira @@aut@@ Moody, Sarah C. @@aut@@ Fietz, Daniela @@aut@@ Indumathy, Sivanjah @@aut@@ Schuppe, Hans-Christian @@aut@@ Hedger, Mark P. @@aut@@ Loveland, Kate L. @@aut@@ |
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Immediately after sex determination in mouse, macrophages enable normal cord formation and removal of inappropriately positioned cells. This study provides new information about macrophages and other immune cells after cord formation in fetal testes, including their density, distribution, and close cellular contacts. C57BL6J mouse testes from embryonic day (E) 13.5 to birth (post-natal day 0; PND0), were examined using immunofluorescence, immunohistochemistry, and RT-qPCR to identify macrophages (F4/80, CD206, MHCII), T cells (CD3), granulocytes/neutrophils (Ly6G), and germ cells (DDX4). F4/$ 80^{+} $ cells were the most abundant, comprising 90% of $ CD45^{+} $ cells at E13.5 and declining to 65% at PND0. Changes in size, shape, and markers (CD206 and MHCII) documented during this interval align with the understanding that F4/$ 80^{+} $ cells have different origins during embryonic life. $ CD3^{+} $ cells and F4/$ 80^{−} $/$ MHCII^{+} $ were absent to rare until PND0. $ Ly6G^{+} $ cells were scarce at E13.5 but increased robustly by PND0 to represent half of the $ CD45^{+} $ cells. These immunofluorescence data were in accord with transcript analysis, which showed that immune marker mRNAs increased with testis age. F4/$ 80^{+} $ and $ Ly6G^{+} $ cells were frequently inside cords adjacent to germ cells at E13.5 and E15.5. F4/$ 80^{+} $ cells were often in clusters next to other immune cells. Macrophages inside cords at E13.5 and E15.5 (F4/$ 80^{Hi} $/$ CD206^{+} $) were different from macrophages at PND0 (F4/$ 80^{Dim} $/$ CD206^{−} $), indicating that they have distinct origins. 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Hosseini, Samira |
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Hosseini, Samira misc Fetal testis development misc Macrophages misc T cells misc Granulocytes misc Immune cell localisation misc Male germ cells The changing landscape of immune cells in the fetal mouse testis |
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The changing landscape of immune cells in the fetal mouse testis Fetal testis development (dpeaa)DE-He213 Macrophages (dpeaa)DE-He213 T cells (dpeaa)DE-He213 Granulocytes (dpeaa)DE-He213 Immune cell localisation (dpeaa)DE-He213 Male germ cells (dpeaa)DE-He213 |
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misc Fetal testis development misc Macrophages misc T cells misc Granulocytes misc Immune cell localisation misc Male germ cells |
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misc Fetal testis development misc Macrophages misc T cells misc Granulocytes misc Immune cell localisation misc Male germ cells |
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The changing landscape of immune cells in the fetal mouse testis |
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Histochemistry and cell biology |
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changing landscape of immune cells in the fetal mouse testis |
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The changing landscape of immune cells in the fetal mouse testis |
| abstract |
Abstract Fetal testis growth involves cell influx and extensive remodeling. Immediately after sex determination in mouse, macrophages enable normal cord formation and removal of inappropriately positioned cells. This study provides new information about macrophages and other immune cells after cord formation in fetal testes, including their density, distribution, and close cellular contacts. C57BL6J mouse testes from embryonic day (E) 13.5 to birth (post-natal day 0; PND0), were examined using immunofluorescence, immunohistochemistry, and RT-qPCR to identify macrophages (F4/80, CD206, MHCII), T cells (CD3), granulocytes/neutrophils (Ly6G), and germ cells (DDX4). F4/$ 80^{+} $ cells were the most abundant, comprising 90% of $ CD45^{+} $ cells at E13.5 and declining to 65% at PND0. Changes in size, shape, and markers (CD206 and MHCII) documented during this interval align with the understanding that F4/$ 80^{+} $ cells have different origins during embryonic life. $ CD3^{+} $ cells and F4/$ 80^{−} $/$ MHCII^{+} $ were absent to rare until PND0. $ Ly6G^{+} $ cells were scarce at E13.5 but increased robustly by PND0 to represent half of the $ CD45^{+} $ cells. These immunofluorescence data were in accord with transcript analysis, which showed that immune marker mRNAs increased with testis age. F4/$ 80^{+} $ and $ Ly6G^{+} $ cells were frequently inside cords adjacent to germ cells at E13.5 and E15.5. F4/$ 80^{+} $ cells were often in clusters next to other immune cells. Macrophages inside cords at E13.5 and E15.5 (F4/$ 80^{Hi} $/$ CD206^{+} $) were different from macrophages at PND0 (F4/$ 80^{Dim} $/$ CD206^{−} $), indicating that they have distinct origins. This histological quantification coupled with transcript information identifies new cellular interactions for immune cells in fetal testis morphogenesis, and highlights new avenues for studies of their functional significance. © The Author(s) 2022 |
| abstractGer |
Abstract Fetal testis growth involves cell influx and extensive remodeling. Immediately after sex determination in mouse, macrophages enable normal cord formation and removal of inappropriately positioned cells. This study provides new information about macrophages and other immune cells after cord formation in fetal testes, including their density, distribution, and close cellular contacts. C57BL6J mouse testes from embryonic day (E) 13.5 to birth (post-natal day 0; PND0), were examined using immunofluorescence, immunohistochemistry, and RT-qPCR to identify macrophages (F4/80, CD206, MHCII), T cells (CD3), granulocytes/neutrophils (Ly6G), and germ cells (DDX4). F4/$ 80^{+} $ cells were the most abundant, comprising 90% of $ CD45^{+} $ cells at E13.5 and declining to 65% at PND0. Changes in size, shape, and markers (CD206 and MHCII) documented during this interval align with the understanding that F4/$ 80^{+} $ cells have different origins during embryonic life. $ CD3^{+} $ cells and F4/$ 80^{−} $/$ MHCII^{+} $ were absent to rare until PND0. $ Ly6G^{+} $ cells were scarce at E13.5 but increased robustly by PND0 to represent half of the $ CD45^{+} $ cells. These immunofluorescence data were in accord with transcript analysis, which showed that immune marker mRNAs increased with testis age. F4/$ 80^{+} $ and $ Ly6G^{+} $ cells were frequently inside cords adjacent to germ cells at E13.5 and E15.5. F4/$ 80^{+} $ cells were often in clusters next to other immune cells. Macrophages inside cords at E13.5 and E15.5 (F4/$ 80^{Hi} $/$ CD206^{+} $) were different from macrophages at PND0 (F4/$ 80^{Dim} $/$ CD206^{−} $), indicating that they have distinct origins. This histological quantification coupled with transcript information identifies new cellular interactions for immune cells in fetal testis morphogenesis, and highlights new avenues for studies of their functional significance. © The Author(s) 2022 |
| abstract_unstemmed |
Abstract Fetal testis growth involves cell influx and extensive remodeling. Immediately after sex determination in mouse, macrophages enable normal cord formation and removal of inappropriately positioned cells. This study provides new information about macrophages and other immune cells after cord formation in fetal testes, including their density, distribution, and close cellular contacts. C57BL6J mouse testes from embryonic day (E) 13.5 to birth (post-natal day 0; PND0), were examined using immunofluorescence, immunohistochemistry, and RT-qPCR to identify macrophages (F4/80, CD206, MHCII), T cells (CD3), granulocytes/neutrophils (Ly6G), and germ cells (DDX4). F4/$ 80^{+} $ cells were the most abundant, comprising 90% of $ CD45^{+} $ cells at E13.5 and declining to 65% at PND0. Changes in size, shape, and markers (CD206 and MHCII) documented during this interval align with the understanding that F4/$ 80^{+} $ cells have different origins during embryonic life. $ CD3^{+} $ cells and F4/$ 80^{−} $/$ MHCII^{+} $ were absent to rare until PND0. $ Ly6G^{+} $ cells were scarce at E13.5 but increased robustly by PND0 to represent half of the $ CD45^{+} $ cells. These immunofluorescence data were in accord with transcript analysis, which showed that immune marker mRNAs increased with testis age. F4/$ 80^{+} $ and $ Ly6G^{+} $ cells were frequently inside cords adjacent to germ cells at E13.5 and E15.5. F4/$ 80^{+} $ cells were often in clusters next to other immune cells. Macrophages inside cords at E13.5 and E15.5 (F4/$ 80^{Hi} $/$ CD206^{+} $) were different from macrophages at PND0 (F4/$ 80^{Dim} $/$ CD206^{−} $), indicating that they have distinct origins. This histological quantification coupled with transcript information identifies new cellular interactions for immune cells in fetal testis morphogenesis, and highlights new avenues for studies of their functional significance. © The Author(s) 2022 |
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| title_short |
The changing landscape of immune cells in the fetal mouse testis |
| url |
https://dx.doi.org/10.1007/s00418-022-02129-6 |
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| author2 |
Moody, Sarah C. Fietz, Daniela Indumathy, Sivanjah Schuppe, Hans-Christian Hedger, Mark P. Loveland, Kate L. |
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Moody, Sarah C. Fietz, Daniela Indumathy, Sivanjah Schuppe, Hans-Christian Hedger, Mark P. Loveland, Kate L. |
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| doi_str |
10.1007/s00418-022-02129-6 |
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2024-07-03T17:43:26.850Z |
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| score |
7.3996468 |