Evaluation of the Anti-amyloidogenic and Fibril-Destabilizing Effects of Salvia officinalis Flower Extract Against Hen’s Egg White Lysozyme: An In Vitro Study
Abstract Amyloid plaques or fibrils are deposits of abnormal proteins that mostly contain β-sheets, which accumulate in different organs and tissues, resulting in impaired tissue function and causing fatal and debilitating diseases. In this study, the effects of Salvia officinalis extract (SOE), pre...
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| Autor*in: |
Faramarzian, Masoumeh [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s), under exclusive licence to Shiraz University 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| Übergeordnetes Werk: |
Enthalten in: Iranian journal of science and technology - Cham, Switzerland : Springer International Pubishing, 2004, 46(2022), 5 vom: 15. Sept., Seite 1349-1358 |
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| Übergeordnetes Werk: |
volume:46 ; year:2022 ; number:5 ; day:15 ; month:09 ; pages:1349-1358 |
| Links: |
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| DOI / URN: |
10.1007/s40995-022-01359-6 |
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| 520 | |a Abstract Amyloid plaques or fibrils are deposits of abnormal proteins that mostly contain β-sheets, which accumulate in different organs and tissues, resulting in impaired tissue function and causing fatal and debilitating diseases. In this study, the effects of Salvia officinalis extract (SOE), prepared from its flowers, on both prevention of amyloid plaques formation and removal of accumulated fibrils were investigated. Therefore, 14 mg/ml of hen egg-white lysozyme (HEWL) was incubated in the absence and presence of 7, 14 and 28 mg/ml of SOE in high temperature and acidic pH to form amyloid fibrils. In this line, thioflavin T (THT), 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence test, Congo red absorbance test, Circular dichroism, and field-emission scanning electron microscopy were employed to study the effect of the extract on these processes. The results indicate that SOE presents a strong anti-amyloidogenic activity. The extract to protein ratio of 0.5:1 (7:14 mg/ml) did not cause the formation of amyloid fibrils in the HEWL solution. However, it resulted in defibrillation of HEWL in a dose-dependent manner. Based on our result, the extract to protein ratio of 1:1 (14:14 mg/ml) was the best ratio to change the structure of long and massive HEWL fibrils to thin and little fibrils. In general, it can be concluded that SOE contains compounds that can be useful for the treatment of amyloid-dependent diseases. | ||
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10.1007/s40995-022-01359-6 doi (DE-627)SPR048434442 (SPR)s40995-022-01359-6-e DE-627 ger DE-627 rakwb eng Faramarzian, Masoumeh verfasserin aut Evaluation of the Anti-amyloidogenic and Fibril-Destabilizing Effects of Salvia officinalis Flower Extract Against Hen’s Egg White Lysozyme: An In Vitro Study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Shiraz University 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Amyloid plaques or fibrils are deposits of abnormal proteins that mostly contain β-sheets, which accumulate in different organs and tissues, resulting in impaired tissue function and causing fatal and debilitating diseases. In this study, the effects of Salvia officinalis extract (SOE), prepared from its flowers, on both prevention of amyloid plaques formation and removal of accumulated fibrils were investigated. Therefore, 14 mg/ml of hen egg-white lysozyme (HEWL) was incubated in the absence and presence of 7, 14 and 28 mg/ml of SOE in high temperature and acidic pH to form amyloid fibrils. In this line, thioflavin T (THT), 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence test, Congo red absorbance test, Circular dichroism, and field-emission scanning electron microscopy were employed to study the effect of the extract on these processes. The results indicate that SOE presents a strong anti-amyloidogenic activity. The extract to protein ratio of 0.5:1 (7:14 mg/ml) did not cause the formation of amyloid fibrils in the HEWL solution. However, it resulted in defibrillation of HEWL in a dose-dependent manner. Based on our result, the extract to protein ratio of 1:1 (14:14 mg/ml) was the best ratio to change the structure of long and massive HEWL fibrils to thin and little fibrils. In general, it can be concluded that SOE contains compounds that can be useful for the treatment of amyloid-dependent diseases. Amyloid fibrils (dpeaa)DE-He213 Amyloid diseases (dpeaa)DE-He213 Biophenols (dpeaa)DE-He213 Bahramikia, Seifollah (orcid)0000-0002-9482-6828 aut Nabi-Afjadi, Mohsen aut Enthalten in Iranian journal of science and technology Cham, Switzerland : Springer International Pubishing, 2004 46(2022), 5 vom: 15. Sept., Seite 1349-1358 (DE-627)SPR038034816 (DE-600)2843077-3 2364-1819 nnns volume:46 year:2022 number:5 day:15 month:09 pages:1349-1358 https://dx.doi.org/10.1007/s40995-022-01359-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 46 2022 5 15 09 1349-1358 |
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10.1007/s40995-022-01359-6 doi (DE-627)SPR048434442 (SPR)s40995-022-01359-6-e DE-627 ger DE-627 rakwb eng Faramarzian, Masoumeh verfasserin aut Evaluation of the Anti-amyloidogenic and Fibril-Destabilizing Effects of Salvia officinalis Flower Extract Against Hen’s Egg White Lysozyme: An In Vitro Study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Shiraz University 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Amyloid plaques or fibrils are deposits of abnormal proteins that mostly contain β-sheets, which accumulate in different organs and tissues, resulting in impaired tissue function and causing fatal and debilitating diseases. In this study, the effects of Salvia officinalis extract (SOE), prepared from its flowers, on both prevention of amyloid plaques formation and removal of accumulated fibrils were investigated. Therefore, 14 mg/ml of hen egg-white lysozyme (HEWL) was incubated in the absence and presence of 7, 14 and 28 mg/ml of SOE in high temperature and acidic pH to form amyloid fibrils. In this line, thioflavin T (THT), 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence test, Congo red absorbance test, Circular dichroism, and field-emission scanning electron microscopy were employed to study the effect of the extract on these processes. The results indicate that SOE presents a strong anti-amyloidogenic activity. The extract to protein ratio of 0.5:1 (7:14 mg/ml) did not cause the formation of amyloid fibrils in the HEWL solution. However, it resulted in defibrillation of HEWL in a dose-dependent manner. Based on our result, the extract to protein ratio of 1:1 (14:14 mg/ml) was the best ratio to change the structure of long and massive HEWL fibrils to thin and little fibrils. In general, it can be concluded that SOE contains compounds that can be useful for the treatment of amyloid-dependent diseases. Amyloid fibrils (dpeaa)DE-He213 Amyloid diseases (dpeaa)DE-He213 Biophenols (dpeaa)DE-He213 Bahramikia, Seifollah (orcid)0000-0002-9482-6828 aut Nabi-Afjadi, Mohsen aut Enthalten in Iranian journal of science and technology Cham, Switzerland : Springer International Pubishing, 2004 46(2022), 5 vom: 15. Sept., Seite 1349-1358 (DE-627)SPR038034816 (DE-600)2843077-3 2364-1819 nnns volume:46 year:2022 number:5 day:15 month:09 pages:1349-1358 https://dx.doi.org/10.1007/s40995-022-01359-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 46 2022 5 15 09 1349-1358 |
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10.1007/s40995-022-01359-6 doi (DE-627)SPR048434442 (SPR)s40995-022-01359-6-e DE-627 ger DE-627 rakwb eng Faramarzian, Masoumeh verfasserin aut Evaluation of the Anti-amyloidogenic and Fibril-Destabilizing Effects of Salvia officinalis Flower Extract Against Hen’s Egg White Lysozyme: An In Vitro Study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Shiraz University 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Amyloid plaques or fibrils are deposits of abnormal proteins that mostly contain β-sheets, which accumulate in different organs and tissues, resulting in impaired tissue function and causing fatal and debilitating diseases. In this study, the effects of Salvia officinalis extract (SOE), prepared from its flowers, on both prevention of amyloid plaques formation and removal of accumulated fibrils were investigated. Therefore, 14 mg/ml of hen egg-white lysozyme (HEWL) was incubated in the absence and presence of 7, 14 and 28 mg/ml of SOE in high temperature and acidic pH to form amyloid fibrils. In this line, thioflavin T (THT), 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence test, Congo red absorbance test, Circular dichroism, and field-emission scanning electron microscopy were employed to study the effect of the extract on these processes. The results indicate that SOE presents a strong anti-amyloidogenic activity. The extract to protein ratio of 0.5:1 (7:14 mg/ml) did not cause the formation of amyloid fibrils in the HEWL solution. However, it resulted in defibrillation of HEWL in a dose-dependent manner. Based on our result, the extract to protein ratio of 1:1 (14:14 mg/ml) was the best ratio to change the structure of long and massive HEWL fibrils to thin and little fibrils. In general, it can be concluded that SOE contains compounds that can be useful for the treatment of amyloid-dependent diseases. Amyloid fibrils (dpeaa)DE-He213 Amyloid diseases (dpeaa)DE-He213 Biophenols (dpeaa)DE-He213 Bahramikia, Seifollah (orcid)0000-0002-9482-6828 aut Nabi-Afjadi, Mohsen aut Enthalten in Iranian journal of science and technology Cham, Switzerland : Springer International Pubishing, 2004 46(2022), 5 vom: 15. Sept., Seite 1349-1358 (DE-627)SPR038034816 (DE-600)2843077-3 2364-1819 nnns volume:46 year:2022 number:5 day:15 month:09 pages:1349-1358 https://dx.doi.org/10.1007/s40995-022-01359-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 46 2022 5 15 09 1349-1358 |
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10.1007/s40995-022-01359-6 doi (DE-627)SPR048434442 (SPR)s40995-022-01359-6-e DE-627 ger DE-627 rakwb eng Faramarzian, Masoumeh verfasserin aut Evaluation of the Anti-amyloidogenic and Fibril-Destabilizing Effects of Salvia officinalis Flower Extract Against Hen’s Egg White Lysozyme: An In Vitro Study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Shiraz University 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Amyloid plaques or fibrils are deposits of abnormal proteins that mostly contain β-sheets, which accumulate in different organs and tissues, resulting in impaired tissue function and causing fatal and debilitating diseases. In this study, the effects of Salvia officinalis extract (SOE), prepared from its flowers, on both prevention of amyloid plaques formation and removal of accumulated fibrils were investigated. Therefore, 14 mg/ml of hen egg-white lysozyme (HEWL) was incubated in the absence and presence of 7, 14 and 28 mg/ml of SOE in high temperature and acidic pH to form amyloid fibrils. In this line, thioflavin T (THT), 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence test, Congo red absorbance test, Circular dichroism, and field-emission scanning electron microscopy were employed to study the effect of the extract on these processes. The results indicate that SOE presents a strong anti-amyloidogenic activity. The extract to protein ratio of 0.5:1 (7:14 mg/ml) did not cause the formation of amyloid fibrils in the HEWL solution. However, it resulted in defibrillation of HEWL in a dose-dependent manner. Based on our result, the extract to protein ratio of 1:1 (14:14 mg/ml) was the best ratio to change the structure of long and massive HEWL fibrils to thin and little fibrils. In general, it can be concluded that SOE contains compounds that can be useful for the treatment of amyloid-dependent diseases. Amyloid fibrils (dpeaa)DE-He213 Amyloid diseases (dpeaa)DE-He213 Biophenols (dpeaa)DE-He213 Bahramikia, Seifollah (orcid)0000-0002-9482-6828 aut Nabi-Afjadi, Mohsen aut Enthalten in Iranian journal of science and technology Cham, Switzerland : Springer International Pubishing, 2004 46(2022), 5 vom: 15. Sept., Seite 1349-1358 (DE-627)SPR038034816 (DE-600)2843077-3 2364-1819 nnns volume:46 year:2022 number:5 day:15 month:09 pages:1349-1358 https://dx.doi.org/10.1007/s40995-022-01359-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 46 2022 5 15 09 1349-1358 |
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10.1007/s40995-022-01359-6 doi (DE-627)SPR048434442 (SPR)s40995-022-01359-6-e DE-627 ger DE-627 rakwb eng Faramarzian, Masoumeh verfasserin aut Evaluation of the Anti-amyloidogenic and Fibril-Destabilizing Effects of Salvia officinalis Flower Extract Against Hen’s Egg White Lysozyme: An In Vitro Study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Shiraz University 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Amyloid plaques or fibrils are deposits of abnormal proteins that mostly contain β-sheets, which accumulate in different organs and tissues, resulting in impaired tissue function and causing fatal and debilitating diseases. In this study, the effects of Salvia officinalis extract (SOE), prepared from its flowers, on both prevention of amyloid plaques formation and removal of accumulated fibrils were investigated. Therefore, 14 mg/ml of hen egg-white lysozyme (HEWL) was incubated in the absence and presence of 7, 14 and 28 mg/ml of SOE in high temperature and acidic pH to form amyloid fibrils. In this line, thioflavin T (THT), 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence test, Congo red absorbance test, Circular dichroism, and field-emission scanning electron microscopy were employed to study the effect of the extract on these processes. The results indicate that SOE presents a strong anti-amyloidogenic activity. The extract to protein ratio of 0.5:1 (7:14 mg/ml) did not cause the formation of amyloid fibrils in the HEWL solution. However, it resulted in defibrillation of HEWL in a dose-dependent manner. Based on our result, the extract to protein ratio of 1:1 (14:14 mg/ml) was the best ratio to change the structure of long and massive HEWL fibrils to thin and little fibrils. In general, it can be concluded that SOE contains compounds that can be useful for the treatment of amyloid-dependent diseases. Amyloid fibrils (dpeaa)DE-He213 Amyloid diseases (dpeaa)DE-He213 Biophenols (dpeaa)DE-He213 Bahramikia, Seifollah (orcid)0000-0002-9482-6828 aut Nabi-Afjadi, Mohsen aut Enthalten in Iranian journal of science and technology Cham, Switzerland : Springer International Pubishing, 2004 46(2022), 5 vom: 15. Sept., Seite 1349-1358 (DE-627)SPR038034816 (DE-600)2843077-3 2364-1819 nnns volume:46 year:2022 number:5 day:15 month:09 pages:1349-1358 https://dx.doi.org/10.1007/s40995-022-01359-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 46 2022 5 15 09 1349-1358 |
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Faramarzian, Masoumeh misc Amyloid fibrils misc Amyloid diseases misc Biophenols Evaluation of the Anti-amyloidogenic and Fibril-Destabilizing Effects of Salvia officinalis Flower Extract Against Hen’s Egg White Lysozyme: An In Vitro Study |
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Evaluation of the Anti-amyloidogenic and Fibril-Destabilizing Effects of Salvia officinalis Flower Extract Against Hen’s Egg White Lysozyme: An In Vitro Study Amyloid fibrils (dpeaa)DE-He213 Amyloid diseases (dpeaa)DE-He213 Biophenols (dpeaa)DE-He213 |
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Evaluation of the Anti-amyloidogenic and Fibril-Destabilizing Effects of Salvia officinalis Flower Extract Against Hen’s Egg White Lysozyme: An In Vitro Study |
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Evaluation of the Anti-amyloidogenic and Fibril-Destabilizing Effects of Salvia officinalis Flower Extract Against Hen’s Egg White Lysozyme: An In Vitro Study |
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Faramarzian, Masoumeh Bahramikia, Seifollah Nabi-Afjadi, Mohsen |
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evaluation of the anti-amyloidogenic and fibril-destabilizing effects of salvia officinalis flower extract against hen’s egg white lysozyme: an in vitro study |
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Evaluation of the Anti-amyloidogenic and Fibril-Destabilizing Effects of Salvia officinalis Flower Extract Against Hen’s Egg White Lysozyme: An In Vitro Study |
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Abstract Amyloid plaques or fibrils are deposits of abnormal proteins that mostly contain β-sheets, which accumulate in different organs and tissues, resulting in impaired tissue function and causing fatal and debilitating diseases. In this study, the effects of Salvia officinalis extract (SOE), prepared from its flowers, on both prevention of amyloid plaques formation and removal of accumulated fibrils were investigated. Therefore, 14 mg/ml of hen egg-white lysozyme (HEWL) was incubated in the absence and presence of 7, 14 and 28 mg/ml of SOE in high temperature and acidic pH to form amyloid fibrils. In this line, thioflavin T (THT), 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence test, Congo red absorbance test, Circular dichroism, and field-emission scanning electron microscopy were employed to study the effect of the extract on these processes. The results indicate that SOE presents a strong anti-amyloidogenic activity. The extract to protein ratio of 0.5:1 (7:14 mg/ml) did not cause the formation of amyloid fibrils in the HEWL solution. However, it resulted in defibrillation of HEWL in a dose-dependent manner. Based on our result, the extract to protein ratio of 1:1 (14:14 mg/ml) was the best ratio to change the structure of long and massive HEWL fibrils to thin and little fibrils. In general, it can be concluded that SOE contains compounds that can be useful for the treatment of amyloid-dependent diseases. © The Author(s), under exclusive licence to Shiraz University 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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Abstract Amyloid plaques or fibrils are deposits of abnormal proteins that mostly contain β-sheets, which accumulate in different organs and tissues, resulting in impaired tissue function and causing fatal and debilitating diseases. In this study, the effects of Salvia officinalis extract (SOE), prepared from its flowers, on both prevention of amyloid plaques formation and removal of accumulated fibrils were investigated. Therefore, 14 mg/ml of hen egg-white lysozyme (HEWL) was incubated in the absence and presence of 7, 14 and 28 mg/ml of SOE in high temperature and acidic pH to form amyloid fibrils. In this line, thioflavin T (THT), 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence test, Congo red absorbance test, Circular dichroism, and field-emission scanning electron microscopy were employed to study the effect of the extract on these processes. The results indicate that SOE presents a strong anti-amyloidogenic activity. The extract to protein ratio of 0.5:1 (7:14 mg/ml) did not cause the formation of amyloid fibrils in the HEWL solution. However, it resulted in defibrillation of HEWL in a dose-dependent manner. Based on our result, the extract to protein ratio of 1:1 (14:14 mg/ml) was the best ratio to change the structure of long and massive HEWL fibrils to thin and little fibrils. In general, it can be concluded that SOE contains compounds that can be useful for the treatment of amyloid-dependent diseases. © The Author(s), under exclusive licence to Shiraz University 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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Abstract Amyloid plaques or fibrils are deposits of abnormal proteins that mostly contain β-sheets, which accumulate in different organs and tissues, resulting in impaired tissue function and causing fatal and debilitating diseases. In this study, the effects of Salvia officinalis extract (SOE), prepared from its flowers, on both prevention of amyloid plaques formation and removal of accumulated fibrils were investigated. Therefore, 14 mg/ml of hen egg-white lysozyme (HEWL) was incubated in the absence and presence of 7, 14 and 28 mg/ml of SOE in high temperature and acidic pH to form amyloid fibrils. In this line, thioflavin T (THT), 1-anilinonaphthalene-8-sulfonic acid (ANS) fluorescence test, Congo red absorbance test, Circular dichroism, and field-emission scanning electron microscopy were employed to study the effect of the extract on these processes. The results indicate that SOE presents a strong anti-amyloidogenic activity. The extract to protein ratio of 0.5:1 (7:14 mg/ml) did not cause the formation of amyloid fibrils in the HEWL solution. However, it resulted in defibrillation of HEWL in a dose-dependent manner. Based on our result, the extract to protein ratio of 1:1 (14:14 mg/ml) was the best ratio to change the structure of long and massive HEWL fibrils to thin and little fibrils. In general, it can be concluded that SOE contains compounds that can be useful for the treatment of amyloid-dependent diseases. © The Author(s), under exclusive licence to Shiraz University 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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Evaluation of the Anti-amyloidogenic and Fibril-Destabilizing Effects of Salvia officinalis Flower Extract Against Hen’s Egg White Lysozyme: An In Vitro Study |
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