Influence of the self-adhering strategy on microhardness, sorption, solubility, color stability, and cytotoxicity compared to bulk-fill and conventional resin composites
Objectives To analyze and compare, in vitro, the microhardness, sorption, solubility, color stability, and cytotoxicity of three types of resin composites: self-adhesive (SARC) (Dyad Flow (DF)/Kerr), bulk-fill (Filtek Bulk Fill Flow (FBF)/3 M ESPE), and conventional (Filtek Z350XT Flow (Z350)/3 M ES...
Ausführliche Beschreibung
Autor*in: |
de Oliveira, Natália Gomes [verfasserIn] de Barros Albuquerque, Amanda Pinheiro de Melo Rêgo, Moacyr Jesus Barreto |
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Englisch |
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2022 |
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Anmerkung: |
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 |
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Übergeordnetes Werk: |
Enthalten in: Clinical Oral Investigations - Springer-Verlag, 2001, 26(2022), 11 vom: 02. Aug., Seite 6663-6670 |
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Übergeordnetes Werk: |
volume:26 ; year:2022 ; number:11 ; day:02 ; month:08 ; pages:6663-6670 |
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DOI / URN: |
10.1007/s00784-022-04624-5 |
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SPR048569100 |
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520 | |a Objectives To analyze and compare, in vitro, the microhardness, sorption, solubility, color stability, and cytotoxicity of three types of resin composites: self-adhesive (SARC) (Dyad Flow (DF)/Kerr), bulk-fill (Filtek Bulk Fill Flow (FBF)/3 M ESPE), and conventional (Filtek Z350XT Flow (Z350)/3 M ESPE). Materials and methods Thirty cylindrical specimens were prepared using a split metal mold (15 mm × 1 mm), divided into 3 groups (n = 10) according to the material used. Vickers hardness (VH) was calculated from three indentations (300gf/15 s) per specimen. The sorption and solubility were measured according to the ISO 4049:2009 specification after storing in distilled water for 7 days. The color of each resin composite was measured using a portable digital spectrophotometer according to the CIELAB system. After a 7-day immersion in coffee, the color variation (∆E) was calculated. Following the ISO 10993:2012, the cytotoxicity in Vero cells was evaluated through the MTT assay. The results were analyzed using the Kruskal–Wallis test to compare the studied groups. The Wilcoxon test was used to compare the assessments in each studied group. For cytotoxicity analysis, the data were compared by the ANOVA test (α = 0.05). Results DF showed the lowest VH (28.67), highest sorption (0.543 µg/$ mm^{3} $) and solubility (1.700 µg/$ mm^{3} $), and higher ∆E after 7 days of coffee immersion (p = 0.008). The resin composites studied were considered non-cytotoxic. Conclusions The SARC presented inferior mechanical and physical–chemical properties than bulk-fill and conventional resin composites, with comparable cytotoxicity against Vero cells. Clinical relevance The simplification of the clinical protocol of SARC can minimize the number of possible failures during the restorative technique. However, considering their inferior physical and mechanical properties, their coverage with materials of higher mechanical properties and physical–chemical stability should be considered. | ||
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10.1007/s00784-022-04624-5 doi (DE-627)SPR048569100 (SPR)s00784-022-04624-5-e DE-627 ger DE-627 rakwb eng de Oliveira, Natália Gomes verfasserin (orcid)0000-0001-6937-1537 aut Influence of the self-adhering strategy on microhardness, sorption, solubility, color stability, and cytotoxicity compared to bulk-fill and conventional resin composites 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 Objectives To analyze and compare, in vitro, the microhardness, sorption, solubility, color stability, and cytotoxicity of three types of resin composites: self-adhesive (SARC) (Dyad Flow (DF)/Kerr), bulk-fill (Filtek Bulk Fill Flow (FBF)/3 M ESPE), and conventional (Filtek Z350XT Flow (Z350)/3 M ESPE). Materials and methods Thirty cylindrical specimens were prepared using a split metal mold (15 mm × 1 mm), divided into 3 groups (n = 10) according to the material used. Vickers hardness (VH) was calculated from three indentations (300gf/15 s) per specimen. The sorption and solubility were measured according to the ISO 4049:2009 specification after storing in distilled water for 7 days. The color of each resin composite was measured using a portable digital spectrophotometer according to the CIELAB system. After a 7-day immersion in coffee, the color variation (∆E) was calculated. Following the ISO 10993:2012, the cytotoxicity in Vero cells was evaluated through the MTT assay. The results were analyzed using the Kruskal–Wallis test to compare the studied groups. The Wilcoxon test was used to compare the assessments in each studied group. For cytotoxicity analysis, the data were compared by the ANOVA test (α = 0.05). Results DF showed the lowest VH (28.67), highest sorption (0.543 µg/$ mm^{3} $) and solubility (1.700 µg/$ mm^{3} $), and higher ∆E after 7 days of coffee immersion (p = 0.008). The resin composites studied were considered non-cytotoxic. Conclusions The SARC presented inferior mechanical and physical–chemical properties than bulk-fill and conventional resin composites, with comparable cytotoxicity against Vero cells. Clinical relevance The simplification of the clinical protocol of SARC can minimize the number of possible failures during the restorative technique. However, considering their inferior physical and mechanical properties, their coverage with materials of higher mechanical properties and physical–chemical stability should be considered. Resin composite (dpeaa)DE-He213 Self-adhering resin composite (dpeaa)DE-He213 Bulk-fill resin composite (dpeaa)DE-He213 Hardness (dpeaa)DE-He213 Sorption (dpeaa)DE-He213 Solubility (dpeaa)DE-He213 Cytotoxicity (dpeaa)DE-He213 Espíndola-Castro, Luís Felipe aut Rocha, Julliana Carvalho aut de Barros Albuquerque, Amanda Pinheiro aut de Melo Rêgo, Moacyr Jesus Barreto aut de Melo Monteiro, Gabriela Queiroz aut de Vasconcelos Carvalho, Marianne aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 26(2022), 11 vom: 02. Aug., Seite 6663-6670 (DE-627)SPR007794231 nnns volume:26 year:2022 number:11 day:02 month:08 pages:6663-6670 https://dx.doi.org/10.1007/s00784-022-04624-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 26 2022 11 02 08 6663-6670 |
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10.1007/s00784-022-04624-5 doi (DE-627)SPR048569100 (SPR)s00784-022-04624-5-e DE-627 ger DE-627 rakwb eng de Oliveira, Natália Gomes verfasserin (orcid)0000-0001-6937-1537 aut Influence of the self-adhering strategy on microhardness, sorption, solubility, color stability, and cytotoxicity compared to bulk-fill and conventional resin composites 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 Objectives To analyze and compare, in vitro, the microhardness, sorption, solubility, color stability, and cytotoxicity of three types of resin composites: self-adhesive (SARC) (Dyad Flow (DF)/Kerr), bulk-fill (Filtek Bulk Fill Flow (FBF)/3 M ESPE), and conventional (Filtek Z350XT Flow (Z350)/3 M ESPE). Materials and methods Thirty cylindrical specimens were prepared using a split metal mold (15 mm × 1 mm), divided into 3 groups (n = 10) according to the material used. Vickers hardness (VH) was calculated from three indentations (300gf/15 s) per specimen. The sorption and solubility were measured according to the ISO 4049:2009 specification after storing in distilled water for 7 days. The color of each resin composite was measured using a portable digital spectrophotometer according to the CIELAB system. After a 7-day immersion in coffee, the color variation (∆E) was calculated. Following the ISO 10993:2012, the cytotoxicity in Vero cells was evaluated through the MTT assay. The results were analyzed using the Kruskal–Wallis test to compare the studied groups. The Wilcoxon test was used to compare the assessments in each studied group. For cytotoxicity analysis, the data were compared by the ANOVA test (α = 0.05). Results DF showed the lowest VH (28.67), highest sorption (0.543 µg/$ mm^{3} $) and solubility (1.700 µg/$ mm^{3} $), and higher ∆E after 7 days of coffee immersion (p = 0.008). The resin composites studied were considered non-cytotoxic. Conclusions The SARC presented inferior mechanical and physical–chemical properties than bulk-fill and conventional resin composites, with comparable cytotoxicity against Vero cells. Clinical relevance The simplification of the clinical protocol of SARC can minimize the number of possible failures during the restorative technique. However, considering their inferior physical and mechanical properties, their coverage with materials of higher mechanical properties and physical–chemical stability should be considered. Resin composite (dpeaa)DE-He213 Self-adhering resin composite (dpeaa)DE-He213 Bulk-fill resin composite (dpeaa)DE-He213 Hardness (dpeaa)DE-He213 Sorption (dpeaa)DE-He213 Solubility (dpeaa)DE-He213 Cytotoxicity (dpeaa)DE-He213 Espíndola-Castro, Luís Felipe aut Rocha, Julliana Carvalho aut de Barros Albuquerque, Amanda Pinheiro aut de Melo Rêgo, Moacyr Jesus Barreto aut de Melo Monteiro, Gabriela Queiroz aut de Vasconcelos Carvalho, Marianne aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 26(2022), 11 vom: 02. Aug., Seite 6663-6670 (DE-627)SPR007794231 nnns volume:26 year:2022 number:11 day:02 month:08 pages:6663-6670 https://dx.doi.org/10.1007/s00784-022-04624-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 26 2022 11 02 08 6663-6670 |
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10.1007/s00784-022-04624-5 doi (DE-627)SPR048569100 (SPR)s00784-022-04624-5-e DE-627 ger DE-627 rakwb eng de Oliveira, Natália Gomes verfasserin (orcid)0000-0001-6937-1537 aut Influence of the self-adhering strategy on microhardness, sorption, solubility, color stability, and cytotoxicity compared to bulk-fill and conventional resin composites 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 Objectives To analyze and compare, in vitro, the microhardness, sorption, solubility, color stability, and cytotoxicity of three types of resin composites: self-adhesive (SARC) (Dyad Flow (DF)/Kerr), bulk-fill (Filtek Bulk Fill Flow (FBF)/3 M ESPE), and conventional (Filtek Z350XT Flow (Z350)/3 M ESPE). Materials and methods Thirty cylindrical specimens were prepared using a split metal mold (15 mm × 1 mm), divided into 3 groups (n = 10) according to the material used. Vickers hardness (VH) was calculated from three indentations (300gf/15 s) per specimen. The sorption and solubility were measured according to the ISO 4049:2009 specification after storing in distilled water for 7 days. The color of each resin composite was measured using a portable digital spectrophotometer according to the CIELAB system. After a 7-day immersion in coffee, the color variation (∆E) was calculated. Following the ISO 10993:2012, the cytotoxicity in Vero cells was evaluated through the MTT assay. The results were analyzed using the Kruskal–Wallis test to compare the studied groups. The Wilcoxon test was used to compare the assessments in each studied group. For cytotoxicity analysis, the data were compared by the ANOVA test (α = 0.05). Results DF showed the lowest VH (28.67), highest sorption (0.543 µg/$ mm^{3} $) and solubility (1.700 µg/$ mm^{3} $), and higher ∆E after 7 days of coffee immersion (p = 0.008). The resin composites studied were considered non-cytotoxic. Conclusions The SARC presented inferior mechanical and physical–chemical properties than bulk-fill and conventional resin composites, with comparable cytotoxicity against Vero cells. Clinical relevance The simplification of the clinical protocol of SARC can minimize the number of possible failures during the restorative technique. However, considering their inferior physical and mechanical properties, their coverage with materials of higher mechanical properties and physical–chemical stability should be considered. Resin composite (dpeaa)DE-He213 Self-adhering resin composite (dpeaa)DE-He213 Bulk-fill resin composite (dpeaa)DE-He213 Hardness (dpeaa)DE-He213 Sorption (dpeaa)DE-He213 Solubility (dpeaa)DE-He213 Cytotoxicity (dpeaa)DE-He213 Espíndola-Castro, Luís Felipe aut Rocha, Julliana Carvalho aut de Barros Albuquerque, Amanda Pinheiro aut de Melo Rêgo, Moacyr Jesus Barreto aut de Melo Monteiro, Gabriela Queiroz aut de Vasconcelos Carvalho, Marianne aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 26(2022), 11 vom: 02. Aug., Seite 6663-6670 (DE-627)SPR007794231 nnns volume:26 year:2022 number:11 day:02 month:08 pages:6663-6670 https://dx.doi.org/10.1007/s00784-022-04624-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 26 2022 11 02 08 6663-6670 |
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10.1007/s00784-022-04624-5 doi (DE-627)SPR048569100 (SPR)s00784-022-04624-5-e DE-627 ger DE-627 rakwb eng de Oliveira, Natália Gomes verfasserin (orcid)0000-0001-6937-1537 aut Influence of the self-adhering strategy on microhardness, sorption, solubility, color stability, and cytotoxicity compared to bulk-fill and conventional resin composites 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 Objectives To analyze and compare, in vitro, the microhardness, sorption, solubility, color stability, and cytotoxicity of three types of resin composites: self-adhesive (SARC) (Dyad Flow (DF)/Kerr), bulk-fill (Filtek Bulk Fill Flow (FBF)/3 M ESPE), and conventional (Filtek Z350XT Flow (Z350)/3 M ESPE). Materials and methods Thirty cylindrical specimens were prepared using a split metal mold (15 mm × 1 mm), divided into 3 groups (n = 10) according to the material used. Vickers hardness (VH) was calculated from three indentations (300gf/15 s) per specimen. The sorption and solubility were measured according to the ISO 4049:2009 specification after storing in distilled water for 7 days. The color of each resin composite was measured using a portable digital spectrophotometer according to the CIELAB system. After a 7-day immersion in coffee, the color variation (∆E) was calculated. Following the ISO 10993:2012, the cytotoxicity in Vero cells was evaluated through the MTT assay. The results were analyzed using the Kruskal–Wallis test to compare the studied groups. The Wilcoxon test was used to compare the assessments in each studied group. For cytotoxicity analysis, the data were compared by the ANOVA test (α = 0.05). Results DF showed the lowest VH (28.67), highest sorption (0.543 µg/$ mm^{3} $) and solubility (1.700 µg/$ mm^{3} $), and higher ∆E after 7 days of coffee immersion (p = 0.008). The resin composites studied were considered non-cytotoxic. Conclusions The SARC presented inferior mechanical and physical–chemical properties than bulk-fill and conventional resin composites, with comparable cytotoxicity against Vero cells. Clinical relevance The simplification of the clinical protocol of SARC can minimize the number of possible failures during the restorative technique. However, considering their inferior physical and mechanical properties, their coverage with materials of higher mechanical properties and physical–chemical stability should be considered. Resin composite (dpeaa)DE-He213 Self-adhering resin composite (dpeaa)DE-He213 Bulk-fill resin composite (dpeaa)DE-He213 Hardness (dpeaa)DE-He213 Sorption (dpeaa)DE-He213 Solubility (dpeaa)DE-He213 Cytotoxicity (dpeaa)DE-He213 Espíndola-Castro, Luís Felipe aut Rocha, Julliana Carvalho aut de Barros Albuquerque, Amanda Pinheiro aut de Melo Rêgo, Moacyr Jesus Barreto aut de Melo Monteiro, Gabriela Queiroz aut de Vasconcelos Carvalho, Marianne aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 26(2022), 11 vom: 02. Aug., Seite 6663-6670 (DE-627)SPR007794231 nnns volume:26 year:2022 number:11 day:02 month:08 pages:6663-6670 https://dx.doi.org/10.1007/s00784-022-04624-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 26 2022 11 02 08 6663-6670 |
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10.1007/s00784-022-04624-5 doi (DE-627)SPR048569100 (SPR)s00784-022-04624-5-e DE-627 ger DE-627 rakwb eng de Oliveira, Natália Gomes verfasserin (orcid)0000-0001-6937-1537 aut Influence of the self-adhering strategy on microhardness, sorption, solubility, color stability, and cytotoxicity compared to bulk-fill and conventional resin composites 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 Objectives To analyze and compare, in vitro, the microhardness, sorption, solubility, color stability, and cytotoxicity of three types of resin composites: self-adhesive (SARC) (Dyad Flow (DF)/Kerr), bulk-fill (Filtek Bulk Fill Flow (FBF)/3 M ESPE), and conventional (Filtek Z350XT Flow (Z350)/3 M ESPE). Materials and methods Thirty cylindrical specimens were prepared using a split metal mold (15 mm × 1 mm), divided into 3 groups (n = 10) according to the material used. Vickers hardness (VH) was calculated from three indentations (300gf/15 s) per specimen. The sorption and solubility were measured according to the ISO 4049:2009 specification after storing in distilled water for 7 days. The color of each resin composite was measured using a portable digital spectrophotometer according to the CIELAB system. After a 7-day immersion in coffee, the color variation (∆E) was calculated. Following the ISO 10993:2012, the cytotoxicity in Vero cells was evaluated through the MTT assay. The results were analyzed using the Kruskal–Wallis test to compare the studied groups. The Wilcoxon test was used to compare the assessments in each studied group. For cytotoxicity analysis, the data were compared by the ANOVA test (α = 0.05). Results DF showed the lowest VH (28.67), highest sorption (0.543 µg/$ mm^{3} $) and solubility (1.700 µg/$ mm^{3} $), and higher ∆E after 7 days of coffee immersion (p = 0.008). The resin composites studied were considered non-cytotoxic. Conclusions The SARC presented inferior mechanical and physical–chemical properties than bulk-fill and conventional resin composites, with comparable cytotoxicity against Vero cells. Clinical relevance The simplification of the clinical protocol of SARC can minimize the number of possible failures during the restorative technique. However, considering their inferior physical and mechanical properties, their coverage with materials of higher mechanical properties and physical–chemical stability should be considered. Resin composite (dpeaa)DE-He213 Self-adhering resin composite (dpeaa)DE-He213 Bulk-fill resin composite (dpeaa)DE-He213 Hardness (dpeaa)DE-He213 Sorption (dpeaa)DE-He213 Solubility (dpeaa)DE-He213 Cytotoxicity (dpeaa)DE-He213 Espíndola-Castro, Luís Felipe aut Rocha, Julliana Carvalho aut de Barros Albuquerque, Amanda Pinheiro aut de Melo Rêgo, Moacyr Jesus Barreto aut de Melo Monteiro, Gabriela Queiroz aut de Vasconcelos Carvalho, Marianne aut Enthalten in Clinical Oral Investigations Springer-Verlag, 2001 26(2022), 11 vom: 02. Aug., Seite 6663-6670 (DE-627)SPR007794231 nnns volume:26 year:2022 number:11 day:02 month:08 pages:6663-6670 https://dx.doi.org/10.1007/s00784-022-04624-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 26 2022 11 02 08 6663-6670 |
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Materials and methods Thirty cylindrical specimens were prepared using a split metal mold (15 mm × 1 mm), divided into 3 groups (n = 10) according to the material used. Vickers hardness (VH) was calculated from three indentations (300gf/15 s) per specimen. The sorption and solubility were measured according to the ISO 4049:2009 specification after storing in distilled water for 7 days. The color of each resin composite was measured using a portable digital spectrophotometer according to the CIELAB system. After a 7-day immersion in coffee, the color variation (∆E) was calculated. Following the ISO 10993:2012, the cytotoxicity in Vero cells was evaluated through the MTT assay. The results were analyzed using the Kruskal–Wallis test to compare the studied groups. The Wilcoxon test was used to compare the assessments in each studied group. For cytotoxicity analysis, the data were compared by the ANOVA test (α = 0.05). 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Influence of the self-adhering strategy on microhardness, sorption, solubility, color stability, and cytotoxicity compared to bulk-fill and conventional resin composites Resin composite (dpeaa)DE-He213 Self-adhering resin composite (dpeaa)DE-He213 Bulk-fill resin composite (dpeaa)DE-He213 Hardness (dpeaa)DE-He213 Sorption (dpeaa)DE-He213 Solubility (dpeaa)DE-He213 Cytotoxicity (dpeaa)DE-He213 |
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de Oliveira, Natália Gomes Espíndola-Castro, Luís Felipe Rocha, Julliana Carvalho de Barros Albuquerque, Amanda Pinheiro de Melo Rêgo, Moacyr Jesus Barreto de Melo Monteiro, Gabriela Queiroz de Vasconcelos Carvalho, Marianne |
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influence of the self-adhering strategy on microhardness, sorption, solubility, color stability, and cytotoxicity compared to bulk-fill and conventional resin composites |
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Influence of the self-adhering strategy on microhardness, sorption, solubility, color stability, and cytotoxicity compared to bulk-fill and conventional resin composites |
abstract |
Objectives To analyze and compare, in vitro, the microhardness, sorption, solubility, color stability, and cytotoxicity of three types of resin composites: self-adhesive (SARC) (Dyad Flow (DF)/Kerr), bulk-fill (Filtek Bulk Fill Flow (FBF)/3 M ESPE), and conventional (Filtek Z350XT Flow (Z350)/3 M ESPE). Materials and methods Thirty cylindrical specimens were prepared using a split metal mold (15 mm × 1 mm), divided into 3 groups (n = 10) according to the material used. Vickers hardness (VH) was calculated from three indentations (300gf/15 s) per specimen. The sorption and solubility were measured according to the ISO 4049:2009 specification after storing in distilled water for 7 days. The color of each resin composite was measured using a portable digital spectrophotometer according to the CIELAB system. After a 7-day immersion in coffee, the color variation (∆E) was calculated. Following the ISO 10993:2012, the cytotoxicity in Vero cells was evaluated through the MTT assay. The results were analyzed using the Kruskal–Wallis test to compare the studied groups. The Wilcoxon test was used to compare the assessments in each studied group. For cytotoxicity analysis, the data were compared by the ANOVA test (α = 0.05). Results DF showed the lowest VH (28.67), highest sorption (0.543 µg/$ mm^{3} $) and solubility (1.700 µg/$ mm^{3} $), and higher ∆E after 7 days of coffee immersion (p = 0.008). The resin composites studied were considered non-cytotoxic. Conclusions The SARC presented inferior mechanical and physical–chemical properties than bulk-fill and conventional resin composites, with comparable cytotoxicity against Vero cells. Clinical relevance The simplification of the clinical protocol of SARC can minimize the number of possible failures during the restorative technique. However, considering their inferior physical and mechanical properties, their coverage with materials of higher mechanical properties and physical–chemical stability should be considered. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 |
abstractGer |
Objectives To analyze and compare, in vitro, the microhardness, sorption, solubility, color stability, and cytotoxicity of three types of resin composites: self-adhesive (SARC) (Dyad Flow (DF)/Kerr), bulk-fill (Filtek Bulk Fill Flow (FBF)/3 M ESPE), and conventional (Filtek Z350XT Flow (Z350)/3 M ESPE). Materials and methods Thirty cylindrical specimens were prepared using a split metal mold (15 mm × 1 mm), divided into 3 groups (n = 10) according to the material used. Vickers hardness (VH) was calculated from three indentations (300gf/15 s) per specimen. The sorption and solubility were measured according to the ISO 4049:2009 specification after storing in distilled water for 7 days. The color of each resin composite was measured using a portable digital spectrophotometer according to the CIELAB system. After a 7-day immersion in coffee, the color variation (∆E) was calculated. Following the ISO 10993:2012, the cytotoxicity in Vero cells was evaluated through the MTT assay. The results were analyzed using the Kruskal–Wallis test to compare the studied groups. The Wilcoxon test was used to compare the assessments in each studied group. For cytotoxicity analysis, the data were compared by the ANOVA test (α = 0.05). Results DF showed the lowest VH (28.67), highest sorption (0.543 µg/$ mm^{3} $) and solubility (1.700 µg/$ mm^{3} $), and higher ∆E after 7 days of coffee immersion (p = 0.008). The resin composites studied were considered non-cytotoxic. Conclusions The SARC presented inferior mechanical and physical–chemical properties than bulk-fill and conventional resin composites, with comparable cytotoxicity against Vero cells. Clinical relevance The simplification of the clinical protocol of SARC can minimize the number of possible failures during the restorative technique. However, considering their inferior physical and mechanical properties, their coverage with materials of higher mechanical properties and physical–chemical stability should be considered. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 |
abstract_unstemmed |
Objectives To analyze and compare, in vitro, the microhardness, sorption, solubility, color stability, and cytotoxicity of three types of resin composites: self-adhesive (SARC) (Dyad Flow (DF)/Kerr), bulk-fill (Filtek Bulk Fill Flow (FBF)/3 M ESPE), and conventional (Filtek Z350XT Flow (Z350)/3 M ESPE). Materials and methods Thirty cylindrical specimens were prepared using a split metal mold (15 mm × 1 mm), divided into 3 groups (n = 10) according to the material used. Vickers hardness (VH) was calculated from three indentations (300gf/15 s) per specimen. The sorption and solubility were measured according to the ISO 4049:2009 specification after storing in distilled water for 7 days. The color of each resin composite was measured using a portable digital spectrophotometer according to the CIELAB system. After a 7-day immersion in coffee, the color variation (∆E) was calculated. Following the ISO 10993:2012, the cytotoxicity in Vero cells was evaluated through the MTT assay. The results were analyzed using the Kruskal–Wallis test to compare the studied groups. The Wilcoxon test was used to compare the assessments in each studied group. For cytotoxicity analysis, the data were compared by the ANOVA test (α = 0.05). Results DF showed the lowest VH (28.67), highest sorption (0.543 µg/$ mm^{3} $) and solubility (1.700 µg/$ mm^{3} $), and higher ∆E after 7 days of coffee immersion (p = 0.008). The resin composites studied were considered non-cytotoxic. Conclusions The SARC presented inferior mechanical and physical–chemical properties than bulk-fill and conventional resin composites, with comparable cytotoxicity against Vero cells. Clinical relevance The simplification of the clinical protocol of SARC can minimize the number of possible failures during the restorative technique. However, considering their inferior physical and mechanical properties, their coverage with materials of higher mechanical properties and physical–chemical stability should be considered. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 |
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