Characterization, expression patterns and functional analysis of PcMPK12 gene in Phytophthora capsici

Abstract Phytophthora capsici is a pathogen of several economically important crops, including pepper (Capsicum annuum). The infection process of P. capsici involves several key developmental steps. This study was conducted to reveal the association between mitogen-activated protein kinases and P. c...
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Autor*in:	Zhu, Tong-tong [verfasserIn] Xiang, Sheng-han Yang, Lei Tang, Fang Li, Wei Liu, Ying-bao Sun, Wen-xiu

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Oomycete MAPK Encystment Pathogenicity

Anmerkung:	© Koninklijke Nederlandse Planteziektenkundige Vereniging 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Übergeordnetes Werk:	Enthalten in: European journal of plant pathology - Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895, 164(2022), 4 vom: 07. Sept., Seite 525-537
Übergeordnetes Werk:	volume:164 ; year:2022 ; number:4 ; day:07 ; month:09 ; pages:525-537

Links:	Volltext

DOI / URN:	10.1007/s10658-022-02576-w

Katalog-ID:	SPR048641464

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520			\|a Abstract Phytophthora capsici is a pathogen of several economically important crops, including pepper (Capsicum annuum). The infection process of P. capsici involves several key developmental steps. This study was conducted to reveal the association between mitogen-activated protein kinases and P. capsici pathogenicity. The PcMPK12 gene was screened by retrieving the P. capsici genome database using the well-characterized Saccharomyces cerevisiae MAPKs as queries. The results of sequence analysis showed that the PcMPK12 gene shared high identity with Phytophthora oomycete MAPKs. It was demonstrated that PcMPK12 was transcribed in each detected stage with higher expression during the zoospore and cyst stages by using qRT-PCR. Pathogen inoculation shows that the PcMPK12 contributed to pathogenicity. Tthe specific segment of PcMPK12 was successfully silenced via RNAi, and transformants displayed deficiency in vegetative growth, oospore production, zoospore release, and tolerance to abiotic stresses. No transformants could infect pepper leaves. Furthermore, DAB stain proved that silenced lines exhibited weakened ability to scavenge ROS at the infected sites. Finally, we proposed that PcMPK12 is a highly conserved MAP kinase signal transducer that is not only required for cyst, zoospore release, and responses to various stresses, but also for ROS detoxification and pathogenicity of P. capsici.
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allfields	10.1007/s10658-022-02576-w doi (DE-627)SPR048641464 (SPR)s10658-022-02576-w-e DE-627 ger DE-627 rakwb eng Zhu, Tong-tong verfasserin aut Characterization, expression patterns and functional analysis of PcMPK12 gene in Phytophthora capsici 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Koninklijke Nederlandse Planteziektenkundige Vereniging 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Phytophthora capsici is a pathogen of several economically important crops, including pepper (Capsicum annuum). The infection process of P. capsici involves several key developmental steps. This study was conducted to reveal the association between mitogen-activated protein kinases and P. capsici pathogenicity. The PcMPK12 gene was screened by retrieving the P. capsici genome database using the well-characterized Saccharomyces cerevisiae MAPKs as queries. The results of sequence analysis showed that the PcMPK12 gene shared high identity with Phytophthora oomycete MAPKs. It was demonstrated that PcMPK12 was transcribed in each detected stage with higher expression during the zoospore and cyst stages by using qRT-PCR. Pathogen inoculation shows that the PcMPK12 contributed to pathogenicity. Tthe specific segment of PcMPK12 was successfully silenced via RNAi, and transformants displayed deficiency in vegetative growth, oospore production, zoospore release, and tolerance to abiotic stresses. No transformants could infect pepper leaves. Furthermore, DAB stain proved that silenced lines exhibited weakened ability to scavenge ROS at the infected sites. Finally, we proposed that PcMPK12 is a highly conserved MAP kinase signal transducer that is not only required for cyst, zoospore release, and responses to various stresses, but also for ROS detoxification and pathogenicity of P. capsici. Oomycete (dpeaa)DE-He213 MAPK (dpeaa)DE-He213 Encystment (dpeaa)DE-He213 Pathogenicity (dpeaa)DE-He213 Xiang, Sheng-han aut Yang, Lei aut Tang, Fang aut Li, Wei aut Liu, Ying-bao aut Sun, Wen-xiu aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 164(2022), 4 vom: 07. Sept., Seite 525-537 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:164 year:2022 number:4 day:07 month:09 pages:525-537 https://dx.doi.org/10.1007/s10658-022-02576-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 164 2022 4 07 09 525-537
spelling	10.1007/s10658-022-02576-w doi (DE-627)SPR048641464 (SPR)s10658-022-02576-w-e DE-627 ger DE-627 rakwb eng Zhu, Tong-tong verfasserin aut Characterization, expression patterns and functional analysis of PcMPK12 gene in Phytophthora capsici 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Koninklijke Nederlandse Planteziektenkundige Vereniging 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Phytophthora capsici is a pathogen of several economically important crops, including pepper (Capsicum annuum). The infection process of P. capsici involves several key developmental steps. This study was conducted to reveal the association between mitogen-activated protein kinases and P. capsici pathogenicity. The PcMPK12 gene was screened by retrieving the P. capsici genome database using the well-characterized Saccharomyces cerevisiae MAPKs as queries. The results of sequence analysis showed that the PcMPK12 gene shared high identity with Phytophthora oomycete MAPKs. It was demonstrated that PcMPK12 was transcribed in each detected stage with higher expression during the zoospore and cyst stages by using qRT-PCR. Pathogen inoculation shows that the PcMPK12 contributed to pathogenicity. Tthe specific segment of PcMPK12 was successfully silenced via RNAi, and transformants displayed deficiency in vegetative growth, oospore production, zoospore release, and tolerance to abiotic stresses. No transformants could infect pepper leaves. Furthermore, DAB stain proved that silenced lines exhibited weakened ability to scavenge ROS at the infected sites. Finally, we proposed that PcMPK12 is a highly conserved MAP kinase signal transducer that is not only required for cyst, zoospore release, and responses to various stresses, but also for ROS detoxification and pathogenicity of P. capsici. Oomycete (dpeaa)DE-He213 MAPK (dpeaa)DE-He213 Encystment (dpeaa)DE-He213 Pathogenicity (dpeaa)DE-He213 Xiang, Sheng-han aut Yang, Lei aut Tang, Fang aut Li, Wei aut Liu, Ying-bao aut Sun, Wen-xiu aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 164(2022), 4 vom: 07. Sept., Seite 525-537 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:164 year:2022 number:4 day:07 month:09 pages:525-537 https://dx.doi.org/10.1007/s10658-022-02576-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 164 2022 4 07 09 525-537
allfields_unstemmed	10.1007/s10658-022-02576-w doi (DE-627)SPR048641464 (SPR)s10658-022-02576-w-e DE-627 ger DE-627 rakwb eng Zhu, Tong-tong verfasserin aut Characterization, expression patterns and functional analysis of PcMPK12 gene in Phytophthora capsici 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Koninklijke Nederlandse Planteziektenkundige Vereniging 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Phytophthora capsici is a pathogen of several economically important crops, including pepper (Capsicum annuum). The infection process of P. capsici involves several key developmental steps. This study was conducted to reveal the association between mitogen-activated protein kinases and P. capsici pathogenicity. The PcMPK12 gene was screened by retrieving the P. capsici genome database using the well-characterized Saccharomyces cerevisiae MAPKs as queries. The results of sequence analysis showed that the PcMPK12 gene shared high identity with Phytophthora oomycete MAPKs. It was demonstrated that PcMPK12 was transcribed in each detected stage with higher expression during the zoospore and cyst stages by using qRT-PCR. Pathogen inoculation shows that the PcMPK12 contributed to pathogenicity. Tthe specific segment of PcMPK12 was successfully silenced via RNAi, and transformants displayed deficiency in vegetative growth, oospore production, zoospore release, and tolerance to abiotic stresses. No transformants could infect pepper leaves. Furthermore, DAB stain proved that silenced lines exhibited weakened ability to scavenge ROS at the infected sites. Finally, we proposed that PcMPK12 is a highly conserved MAP kinase signal transducer that is not only required for cyst, zoospore release, and responses to various stresses, but also for ROS detoxification and pathogenicity of P. capsici. Oomycete (dpeaa)DE-He213 MAPK (dpeaa)DE-He213 Encystment (dpeaa)DE-He213 Pathogenicity (dpeaa)DE-He213 Xiang, Sheng-han aut Yang, Lei aut Tang, Fang aut Li, Wei aut Liu, Ying-bao aut Sun, Wen-xiu aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 164(2022), 4 vom: 07. Sept., Seite 525-537 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:164 year:2022 number:4 day:07 month:09 pages:525-537 https://dx.doi.org/10.1007/s10658-022-02576-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 164 2022 4 07 09 525-537
allfieldsGer	10.1007/s10658-022-02576-w doi (DE-627)SPR048641464 (SPR)s10658-022-02576-w-e DE-627 ger DE-627 rakwb eng Zhu, Tong-tong verfasserin aut Characterization, expression patterns and functional analysis of PcMPK12 gene in Phytophthora capsici 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Koninklijke Nederlandse Planteziektenkundige Vereniging 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Phytophthora capsici is a pathogen of several economically important crops, including pepper (Capsicum annuum). The infection process of P. capsici involves several key developmental steps. This study was conducted to reveal the association between mitogen-activated protein kinases and P. capsici pathogenicity. The PcMPK12 gene was screened by retrieving the P. capsici genome database using the well-characterized Saccharomyces cerevisiae MAPKs as queries. The results of sequence analysis showed that the PcMPK12 gene shared high identity with Phytophthora oomycete MAPKs. It was demonstrated that PcMPK12 was transcribed in each detected stage with higher expression during the zoospore and cyst stages by using qRT-PCR. Pathogen inoculation shows that the PcMPK12 contributed to pathogenicity. Tthe specific segment of PcMPK12 was successfully silenced via RNAi, and transformants displayed deficiency in vegetative growth, oospore production, zoospore release, and tolerance to abiotic stresses. No transformants could infect pepper leaves. Furthermore, DAB stain proved that silenced lines exhibited weakened ability to scavenge ROS at the infected sites. Finally, we proposed that PcMPK12 is a highly conserved MAP kinase signal transducer that is not only required for cyst, zoospore release, and responses to various stresses, but also for ROS detoxification and pathogenicity of P. capsici. Oomycete (dpeaa)DE-He213 MAPK (dpeaa)DE-He213 Encystment (dpeaa)DE-He213 Pathogenicity (dpeaa)DE-He213 Xiang, Sheng-han aut Yang, Lei aut Tang, Fang aut Li, Wei aut Liu, Ying-bao aut Sun, Wen-xiu aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 164(2022), 4 vom: 07. Sept., Seite 525-537 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:164 year:2022 number:4 day:07 month:09 pages:525-537 https://dx.doi.org/10.1007/s10658-022-02576-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 164 2022 4 07 09 525-537
allfieldsSound	10.1007/s10658-022-02576-w doi (DE-627)SPR048641464 (SPR)s10658-022-02576-w-e DE-627 ger DE-627 rakwb eng Zhu, Tong-tong verfasserin aut Characterization, expression patterns and functional analysis of PcMPK12 gene in Phytophthora capsici 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Koninklijke Nederlandse Planteziektenkundige Vereniging 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Phytophthora capsici is a pathogen of several economically important crops, including pepper (Capsicum annuum). The infection process of P. capsici involves several key developmental steps. This study was conducted to reveal the association between mitogen-activated protein kinases and P. capsici pathogenicity. The PcMPK12 gene was screened by retrieving the P. capsici genome database using the well-characterized Saccharomyces cerevisiae MAPKs as queries. The results of sequence analysis showed that the PcMPK12 gene shared high identity with Phytophthora oomycete MAPKs. It was demonstrated that PcMPK12 was transcribed in each detected stage with higher expression during the zoospore and cyst stages by using qRT-PCR. Pathogen inoculation shows that the PcMPK12 contributed to pathogenicity. Tthe specific segment of PcMPK12 was successfully silenced via RNAi, and transformants displayed deficiency in vegetative growth, oospore production, zoospore release, and tolerance to abiotic stresses. No transformants could infect pepper leaves. Furthermore, DAB stain proved that silenced lines exhibited weakened ability to scavenge ROS at the infected sites. Finally, we proposed that PcMPK12 is a highly conserved MAP kinase signal transducer that is not only required for cyst, zoospore release, and responses to various stresses, but also for ROS detoxification and pathogenicity of P. capsici. Oomycete (dpeaa)DE-He213 MAPK (dpeaa)DE-He213 Encystment (dpeaa)DE-He213 Pathogenicity (dpeaa)DE-He213 Xiang, Sheng-han aut Yang, Lei aut Tang, Fang aut Li, Wei aut Liu, Ying-bao aut Sun, Wen-xiu aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 164(2022), 4 vom: 07. Sept., Seite 525-537 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:164 year:2022 number:4 day:07 month:09 pages:525-537 https://dx.doi.org/10.1007/s10658-022-02576-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 164 2022 4 07 09 525-537
language	English
source	Enthalten in European journal of plant pathology 164(2022), 4 vom: 07. Sept., Seite 525-537 volume:164 year:2022 number:4 day:07 month:09 pages:525-537
sourceStr	Enthalten in European journal of plant pathology 164(2022), 4 vom: 07. Sept., Seite 525-537 volume:164 year:2022 number:4 day:07 month:09 pages:525-537
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Oomycete MAPK Encystment Pathogenicity
isfreeaccess_bool	false
container_title	European journal of plant pathology
authorswithroles_txt_mv	Zhu, Tong-tong @@aut@@ Xiang, Sheng-han @@aut@@ Yang, Lei @@aut@@ Tang, Fang @@aut@@ Li, Wei @@aut@@ Liu, Ying-bao @@aut@@ Sun, Wen-xiu @@aut@@
publishDateDaySort_date	2022-09-07T00:00:00Z
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author	Zhu, Tong-tong
spellingShingle	Zhu, Tong-tong misc Oomycete misc MAPK misc Encystment misc Pathogenicity Characterization, expression patterns and functional analysis of PcMPK12 gene in Phytophthora capsici
authorStr	Zhu, Tong-tong
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topic_title	Characterization, expression patterns and functional analysis of PcMPK12 gene in Phytophthora capsici Oomycete (dpeaa)DE-He213 MAPK (dpeaa)DE-He213 Encystment (dpeaa)DE-He213 Pathogenicity (dpeaa)DE-He213
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title	Characterization, expression patterns and functional analysis of PcMPK12 gene in Phytophthora capsici
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title_full	Characterization, expression patterns and functional analysis of PcMPK12 gene in Phytophthora capsici
author_sort	Zhu, Tong-tong
journal	European journal of plant pathology
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author_browse	Zhu, Tong-tong Xiang, Sheng-han Yang, Lei Tang, Fang Li, Wei Liu, Ying-bao Sun, Wen-xiu
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doi_str_mv	10.1007/s10658-022-02576-w
title_sort	characterization, expression patterns and functional analysis of pcmpk12 gene in phytophthora capsici
title_auth	Characterization, expression patterns and functional analysis of PcMPK12 gene in Phytophthora capsici
abstract	Abstract Phytophthora capsici is a pathogen of several economically important crops, including pepper (Capsicum annuum). The infection process of P. capsici involves several key developmental steps. This study was conducted to reveal the association between mitogen-activated protein kinases and P. capsici pathogenicity. The PcMPK12 gene was screened by retrieving the P. capsici genome database using the well-characterized Saccharomyces cerevisiae MAPKs as queries. The results of sequence analysis showed that the PcMPK12 gene shared high identity with Phytophthora oomycete MAPKs. It was demonstrated that PcMPK12 was transcribed in each detected stage with higher expression during the zoospore and cyst stages by using qRT-PCR. Pathogen inoculation shows that the PcMPK12 contributed to pathogenicity. Tthe specific segment of PcMPK12 was successfully silenced via RNAi, and transformants displayed deficiency in vegetative growth, oospore production, zoospore release, and tolerance to abiotic stresses. No transformants could infect pepper leaves. Furthermore, DAB stain proved that silenced lines exhibited weakened ability to scavenge ROS at the infected sites. Finally, we proposed that PcMPK12 is a highly conserved MAP kinase signal transducer that is not only required for cyst, zoospore release, and responses to various stresses, but also for ROS detoxification and pathogenicity of P. capsici. © Koninklijke Nederlandse Planteziektenkundige Vereniging 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
abstractGer	Abstract Phytophthora capsici is a pathogen of several economically important crops, including pepper (Capsicum annuum). The infection process of P. capsici involves several key developmental steps. This study was conducted to reveal the association between mitogen-activated protein kinases and P. capsici pathogenicity. The PcMPK12 gene was screened by retrieving the P. capsici genome database using the well-characterized Saccharomyces cerevisiae MAPKs as queries. The results of sequence analysis showed that the PcMPK12 gene shared high identity with Phytophthora oomycete MAPKs. It was demonstrated that PcMPK12 was transcribed in each detected stage with higher expression during the zoospore and cyst stages by using qRT-PCR. Pathogen inoculation shows that the PcMPK12 contributed to pathogenicity. Tthe specific segment of PcMPK12 was successfully silenced via RNAi, and transformants displayed deficiency in vegetative growth, oospore production, zoospore release, and tolerance to abiotic stresses. No transformants could infect pepper leaves. Furthermore, DAB stain proved that silenced lines exhibited weakened ability to scavenge ROS at the infected sites. Finally, we proposed that PcMPK12 is a highly conserved MAP kinase signal transducer that is not only required for cyst, zoospore release, and responses to various stresses, but also for ROS detoxification and pathogenicity of P. capsici. © Koninklijke Nederlandse Planteziektenkundige Vereniging 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
abstract_unstemmed	Abstract Phytophthora capsici is a pathogen of several economically important crops, including pepper (Capsicum annuum). The infection process of P. capsici involves several key developmental steps. This study was conducted to reveal the association between mitogen-activated protein kinases and P. capsici pathogenicity. The PcMPK12 gene was screened by retrieving the P. capsici genome database using the well-characterized Saccharomyces cerevisiae MAPKs as queries. The results of sequence analysis showed that the PcMPK12 gene shared high identity with Phytophthora oomycete MAPKs. It was demonstrated that PcMPK12 was transcribed in each detected stage with higher expression during the zoospore and cyst stages by using qRT-PCR. Pathogen inoculation shows that the PcMPK12 contributed to pathogenicity. Tthe specific segment of PcMPK12 was successfully silenced via RNAi, and transformants displayed deficiency in vegetative growth, oospore production, zoospore release, and tolerance to abiotic stresses. No transformants could infect pepper leaves. Furthermore, DAB stain proved that silenced lines exhibited weakened ability to scavenge ROS at the infected sites. Finally, we proposed that PcMPK12 is a highly conserved MAP kinase signal transducer that is not only required for cyst, zoospore release, and responses to various stresses, but also for ROS detoxification and pathogenicity of P. capsici. © Koninklijke Nederlandse Planteziektenkundige Vereniging 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
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title_short	Characterization, expression patterns and functional analysis of PcMPK12 gene in Phytophthora capsici
url	https://dx.doi.org/10.1007/s10658-022-02576-w
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author2	Xiang, Sheng-han Yang, Lei Tang, Fang Li, Wei Liu, Ying-bao Sun, Wen-xiu
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Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Phytophthora capsici is a pathogen of several economically important crops, including pepper (Capsicum annuum). The infection process of P. capsici involves several key developmental steps. This study was conducted to reveal the association between mitogen-activated protein kinases and P. capsici pathogenicity. The PcMPK12 gene was screened by retrieving the P. capsici genome database using the well-characterized Saccharomyces cerevisiae MAPKs as queries. The results of sequence analysis showed that the PcMPK12 gene shared high identity with Phytophthora oomycete MAPKs. It was demonstrated that PcMPK12 was transcribed in each detected stage with higher expression during the zoospore and cyst stages by using qRT-PCR. Pathogen inoculation shows that the PcMPK12 contributed to pathogenicity. Tthe specific segment of PcMPK12 was successfully silenced via RNAi, and transformants displayed deficiency in vegetative growth, oospore production, zoospore release, and tolerance to abiotic stresses. No transformants could infect pepper leaves. Furthermore, DAB stain proved that silenced lines exhibited weakened ability to scavenge ROS at the infected sites. 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