Identification of Saccharum CaM gene family and function characterization of ScCaM1 during cold and oxidant exposure in Pichia pastoris
Background Calmodulin (CaM) plays an essential role in binding calcium ions and mediating the interpretation of $ Ca^{2+} $ signals in plants under various stresses. However, the evolutionary relationship of CaM family proteins in Saccharum has not been elucidated. Objective To deduce and explore th...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Wang, Hengbo [verfasserIn] |
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E-Artikel |
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| Sprache: |
Englisch |
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2022 |
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© The Author(s) under exclusive licence to The Genetics Society of Korea 2022 |
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| Übergeordnetes Werk: |
Enthalten in: Genes & Genomics - The Genetics Society of Korea, 2010, 45(2022), 1 vom: 24. Mai, Seite 103-122 |
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| Übergeordnetes Werk: |
volume:45 ; year:2022 ; number:1 ; day:24 ; month:05 ; pages:103-122 |
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| DOI / URN: |
10.1007/s13258-022-01263-8 |
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SPR048986127 |
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| 520 | |a Background Calmodulin (CaM) plays an essential role in binding calcium ions and mediating the interpretation of $ Ca^{2+} $ signals in plants under various stresses. However, the evolutionary relationship of CaM family proteins in Saccharum has not been elucidated. Objective To deduce and explore the evolution and function of Saccharum CaM family. Methods A total of 104 typical CaMs were obtained from Saccharum spontaneum and other 18 plant species. The molecular characteristics and evolution of those CaM proteins were analyzed. A typical CaM gene, ScCaM1, was subsequently cloned from sugarcane (Saccharum spp. hybrid). Its expression patterns in different tissues and under various abiotic stresses were assessed by quantitative real-time PCR. Then the green fluorescent protein was used to determine the subcellular localization of ScCaM1. Finally, the function of ScCaM1 was evaluated via heterologous yeast expression systems. Results Three typical CaM members (SsCaM1, SsCaM2, and SsCaM3) were identified from the S. spontaneum genome database. CaMs were originated from the two last common ancestors before the origin of angiosperms. The number of CaM family members did not correlate to the genome size but correlated with allopolyploidization events. The ScCaM1 was more highly expressed in buds and roots than in other tissues. The expression patterns of ScCaM1 suggested that it was involved in responses to various abiotic stresses in sugarcane via different hormonal signaling pathways. Noteworthily, its expression levels appeared relatively stable during the cold exposure in the cold-tolerant variety but significantly suppressed in the cold-susceptible variety. Moreover, the recombinant yeast (Pichia pastoris) overexpressing ScCaM1 grew better than the wild-type yeast strain under cold and oxidative stresses. It was revealed that the ScCaM1 played a positive role in reactive oxygen species scavenging and conferred enhanced cold and oxidative stress tolerance to cells. Conclusion This study provided comprehensive information on the CaM gene family in Saccharum and would facilitate further investigation of their functional characterization. | ||
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| 650 | 4 | |a Molecular evolution |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Cold stress |7 (dpeaa)DE-He213 | |
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| 700 | 1 | |a Que, Youxiong |4 aut | |
| 700 | 1 | |a Xu, Liping |4 aut | |
| 700 | 1 | |a Guo, Jinlong |4 aut | |
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10.1007/s13258-022-01263-8 doi (DE-627)SPR048986127 (SPR)s13258-022-01263-8-e DE-627 ger DE-627 rakwb eng Wang, Hengbo verfasserin aut Identification of Saccharum CaM gene family and function characterization of ScCaM1 during cold and oxidant exposure in Pichia pastoris 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to The Genetics Society of Korea 2022 Background Calmodulin (CaM) plays an essential role in binding calcium ions and mediating the interpretation of $ Ca^{2+} $ signals in plants under various stresses. However, the evolutionary relationship of CaM family proteins in Saccharum has not been elucidated. Objective To deduce and explore the evolution and function of Saccharum CaM family. Methods A total of 104 typical CaMs were obtained from Saccharum spontaneum and other 18 plant species. The molecular characteristics and evolution of those CaM proteins were analyzed. A typical CaM gene, ScCaM1, was subsequently cloned from sugarcane (Saccharum spp. hybrid). Its expression patterns in different tissues and under various abiotic stresses were assessed by quantitative real-time PCR. Then the green fluorescent protein was used to determine the subcellular localization of ScCaM1. Finally, the function of ScCaM1 was evaluated via heterologous yeast expression systems. Results Three typical CaM members (SsCaM1, SsCaM2, and SsCaM3) were identified from the S. spontaneum genome database. CaMs were originated from the two last common ancestors before the origin of angiosperms. The number of CaM family members did not correlate to the genome size but correlated with allopolyploidization events. The ScCaM1 was more highly expressed in buds and roots than in other tissues. The expression patterns of ScCaM1 suggested that it was involved in responses to various abiotic stresses in sugarcane via different hormonal signaling pathways. Noteworthily, its expression levels appeared relatively stable during the cold exposure in the cold-tolerant variety but significantly suppressed in the cold-susceptible variety. Moreover, the recombinant yeast (Pichia pastoris) overexpressing ScCaM1 grew better than the wild-type yeast strain under cold and oxidative stresses. It was revealed that the ScCaM1 played a positive role in reactive oxygen species scavenging and conferred enhanced cold and oxidative stress tolerance to cells. Conclusion This study provided comprehensive information on the CaM gene family in Saccharum and would facilitate further investigation of their functional characterization. Calmodulin (dpeaa)DE-He213 Molecular evolution (dpeaa)DE-He213 Cold stress (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Feng, Meichang aut Zhong, Xiaoqiang aut Yu, Qing aut Que, Youxiong aut Xu, Liping aut Guo, Jinlong aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 45(2022), 1 vom: 24. Mai, Seite 103-122 (DE-627)SPR031096425 nnns volume:45 year:2022 number:1 day:24 month:05 pages:103-122 https://dx.doi.org/10.1007/s13258-022-01263-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 45 2022 1 24 05 103-122 |
| spelling |
10.1007/s13258-022-01263-8 doi (DE-627)SPR048986127 (SPR)s13258-022-01263-8-e DE-627 ger DE-627 rakwb eng Wang, Hengbo verfasserin aut Identification of Saccharum CaM gene family and function characterization of ScCaM1 during cold and oxidant exposure in Pichia pastoris 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to The Genetics Society of Korea 2022 Background Calmodulin (CaM) plays an essential role in binding calcium ions and mediating the interpretation of $ Ca^{2+} $ signals in plants under various stresses. However, the evolutionary relationship of CaM family proteins in Saccharum has not been elucidated. Objective To deduce and explore the evolution and function of Saccharum CaM family. Methods A total of 104 typical CaMs were obtained from Saccharum spontaneum and other 18 plant species. The molecular characteristics and evolution of those CaM proteins were analyzed. A typical CaM gene, ScCaM1, was subsequently cloned from sugarcane (Saccharum spp. hybrid). Its expression patterns in different tissues and under various abiotic stresses were assessed by quantitative real-time PCR. Then the green fluorescent protein was used to determine the subcellular localization of ScCaM1. Finally, the function of ScCaM1 was evaluated via heterologous yeast expression systems. Results Three typical CaM members (SsCaM1, SsCaM2, and SsCaM3) were identified from the S. spontaneum genome database. CaMs were originated from the two last common ancestors before the origin of angiosperms. The number of CaM family members did not correlate to the genome size but correlated with allopolyploidization events. The ScCaM1 was more highly expressed in buds and roots than in other tissues. The expression patterns of ScCaM1 suggested that it was involved in responses to various abiotic stresses in sugarcane via different hormonal signaling pathways. Noteworthily, its expression levels appeared relatively stable during the cold exposure in the cold-tolerant variety but significantly suppressed in the cold-susceptible variety. Moreover, the recombinant yeast (Pichia pastoris) overexpressing ScCaM1 grew better than the wild-type yeast strain under cold and oxidative stresses. It was revealed that the ScCaM1 played a positive role in reactive oxygen species scavenging and conferred enhanced cold and oxidative stress tolerance to cells. Conclusion This study provided comprehensive information on the CaM gene family in Saccharum and would facilitate further investigation of their functional characterization. Calmodulin (dpeaa)DE-He213 Molecular evolution (dpeaa)DE-He213 Cold stress (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Feng, Meichang aut Zhong, Xiaoqiang aut Yu, Qing aut Que, Youxiong aut Xu, Liping aut Guo, Jinlong aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 45(2022), 1 vom: 24. Mai, Seite 103-122 (DE-627)SPR031096425 nnns volume:45 year:2022 number:1 day:24 month:05 pages:103-122 https://dx.doi.org/10.1007/s13258-022-01263-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 45 2022 1 24 05 103-122 |
| allfields_unstemmed |
10.1007/s13258-022-01263-8 doi (DE-627)SPR048986127 (SPR)s13258-022-01263-8-e DE-627 ger DE-627 rakwb eng Wang, Hengbo verfasserin aut Identification of Saccharum CaM gene family and function characterization of ScCaM1 during cold and oxidant exposure in Pichia pastoris 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to The Genetics Society of Korea 2022 Background Calmodulin (CaM) plays an essential role in binding calcium ions and mediating the interpretation of $ Ca^{2+} $ signals in plants under various stresses. However, the evolutionary relationship of CaM family proteins in Saccharum has not been elucidated. Objective To deduce and explore the evolution and function of Saccharum CaM family. Methods A total of 104 typical CaMs were obtained from Saccharum spontaneum and other 18 plant species. The molecular characteristics and evolution of those CaM proteins were analyzed. A typical CaM gene, ScCaM1, was subsequently cloned from sugarcane (Saccharum spp. hybrid). Its expression patterns in different tissues and under various abiotic stresses were assessed by quantitative real-time PCR. Then the green fluorescent protein was used to determine the subcellular localization of ScCaM1. Finally, the function of ScCaM1 was evaluated via heterologous yeast expression systems. Results Three typical CaM members (SsCaM1, SsCaM2, and SsCaM3) were identified from the S. spontaneum genome database. CaMs were originated from the two last common ancestors before the origin of angiosperms. The number of CaM family members did not correlate to the genome size but correlated with allopolyploidization events. The ScCaM1 was more highly expressed in buds and roots than in other tissues. The expression patterns of ScCaM1 suggested that it was involved in responses to various abiotic stresses in sugarcane via different hormonal signaling pathways. Noteworthily, its expression levels appeared relatively stable during the cold exposure in the cold-tolerant variety but significantly suppressed in the cold-susceptible variety. Moreover, the recombinant yeast (Pichia pastoris) overexpressing ScCaM1 grew better than the wild-type yeast strain under cold and oxidative stresses. It was revealed that the ScCaM1 played a positive role in reactive oxygen species scavenging and conferred enhanced cold and oxidative stress tolerance to cells. Conclusion This study provided comprehensive information on the CaM gene family in Saccharum and would facilitate further investigation of their functional characterization. Calmodulin (dpeaa)DE-He213 Molecular evolution (dpeaa)DE-He213 Cold stress (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Feng, Meichang aut Zhong, Xiaoqiang aut Yu, Qing aut Que, Youxiong aut Xu, Liping aut Guo, Jinlong aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 45(2022), 1 vom: 24. Mai, Seite 103-122 (DE-627)SPR031096425 nnns volume:45 year:2022 number:1 day:24 month:05 pages:103-122 https://dx.doi.org/10.1007/s13258-022-01263-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 45 2022 1 24 05 103-122 |
| allfieldsGer |
10.1007/s13258-022-01263-8 doi (DE-627)SPR048986127 (SPR)s13258-022-01263-8-e DE-627 ger DE-627 rakwb eng Wang, Hengbo verfasserin aut Identification of Saccharum CaM gene family and function characterization of ScCaM1 during cold and oxidant exposure in Pichia pastoris 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to The Genetics Society of Korea 2022 Background Calmodulin (CaM) plays an essential role in binding calcium ions and mediating the interpretation of $ Ca^{2+} $ signals in plants under various stresses. However, the evolutionary relationship of CaM family proteins in Saccharum has not been elucidated. Objective To deduce and explore the evolution and function of Saccharum CaM family. Methods A total of 104 typical CaMs were obtained from Saccharum spontaneum and other 18 plant species. The molecular characteristics and evolution of those CaM proteins were analyzed. A typical CaM gene, ScCaM1, was subsequently cloned from sugarcane (Saccharum spp. hybrid). Its expression patterns in different tissues and under various abiotic stresses were assessed by quantitative real-time PCR. Then the green fluorescent protein was used to determine the subcellular localization of ScCaM1. Finally, the function of ScCaM1 was evaluated via heterologous yeast expression systems. Results Three typical CaM members (SsCaM1, SsCaM2, and SsCaM3) were identified from the S. spontaneum genome database. CaMs were originated from the two last common ancestors before the origin of angiosperms. The number of CaM family members did not correlate to the genome size but correlated with allopolyploidization events. The ScCaM1 was more highly expressed in buds and roots than in other tissues. The expression patterns of ScCaM1 suggested that it was involved in responses to various abiotic stresses in sugarcane via different hormonal signaling pathways. Noteworthily, its expression levels appeared relatively stable during the cold exposure in the cold-tolerant variety but significantly suppressed in the cold-susceptible variety. Moreover, the recombinant yeast (Pichia pastoris) overexpressing ScCaM1 grew better than the wild-type yeast strain under cold and oxidative stresses. It was revealed that the ScCaM1 played a positive role in reactive oxygen species scavenging and conferred enhanced cold and oxidative stress tolerance to cells. Conclusion This study provided comprehensive information on the CaM gene family in Saccharum and would facilitate further investigation of their functional characterization. Calmodulin (dpeaa)DE-He213 Molecular evolution (dpeaa)DE-He213 Cold stress (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Feng, Meichang aut Zhong, Xiaoqiang aut Yu, Qing aut Que, Youxiong aut Xu, Liping aut Guo, Jinlong aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 45(2022), 1 vom: 24. Mai, Seite 103-122 (DE-627)SPR031096425 nnns volume:45 year:2022 number:1 day:24 month:05 pages:103-122 https://dx.doi.org/10.1007/s13258-022-01263-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 45 2022 1 24 05 103-122 |
| allfieldsSound |
10.1007/s13258-022-01263-8 doi (DE-627)SPR048986127 (SPR)s13258-022-01263-8-e DE-627 ger DE-627 rakwb eng Wang, Hengbo verfasserin aut Identification of Saccharum CaM gene family and function characterization of ScCaM1 during cold and oxidant exposure in Pichia pastoris 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to The Genetics Society of Korea 2022 Background Calmodulin (CaM) plays an essential role in binding calcium ions and mediating the interpretation of $ Ca^{2+} $ signals in plants under various stresses. However, the evolutionary relationship of CaM family proteins in Saccharum has not been elucidated. Objective To deduce and explore the evolution and function of Saccharum CaM family. Methods A total of 104 typical CaMs were obtained from Saccharum spontaneum and other 18 plant species. The molecular characteristics and evolution of those CaM proteins were analyzed. A typical CaM gene, ScCaM1, was subsequently cloned from sugarcane (Saccharum spp. hybrid). Its expression patterns in different tissues and under various abiotic stresses were assessed by quantitative real-time PCR. Then the green fluorescent protein was used to determine the subcellular localization of ScCaM1. Finally, the function of ScCaM1 was evaluated via heterologous yeast expression systems. Results Three typical CaM members (SsCaM1, SsCaM2, and SsCaM3) were identified from the S. spontaneum genome database. CaMs were originated from the two last common ancestors before the origin of angiosperms. The number of CaM family members did not correlate to the genome size but correlated with allopolyploidization events. The ScCaM1 was more highly expressed in buds and roots than in other tissues. The expression patterns of ScCaM1 suggested that it was involved in responses to various abiotic stresses in sugarcane via different hormonal signaling pathways. Noteworthily, its expression levels appeared relatively stable during the cold exposure in the cold-tolerant variety but significantly suppressed in the cold-susceptible variety. Moreover, the recombinant yeast (Pichia pastoris) overexpressing ScCaM1 grew better than the wild-type yeast strain under cold and oxidative stresses. It was revealed that the ScCaM1 played a positive role in reactive oxygen species scavenging and conferred enhanced cold and oxidative stress tolerance to cells. Conclusion This study provided comprehensive information on the CaM gene family in Saccharum and would facilitate further investigation of their functional characterization. Calmodulin (dpeaa)DE-He213 Molecular evolution (dpeaa)DE-He213 Cold stress (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Feng, Meichang aut Zhong, Xiaoqiang aut Yu, Qing aut Que, Youxiong aut Xu, Liping aut Guo, Jinlong aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 45(2022), 1 vom: 24. Mai, Seite 103-122 (DE-627)SPR031096425 nnns volume:45 year:2022 number:1 day:24 month:05 pages:103-122 https://dx.doi.org/10.1007/s13258-022-01263-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 45 2022 1 24 05 103-122 |
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However, the evolutionary relationship of CaM family proteins in Saccharum has not been elucidated. Objective To deduce and explore the evolution and function of Saccharum CaM family. Methods A total of 104 typical CaMs were obtained from Saccharum spontaneum and other 18 plant species. The molecular characteristics and evolution of those CaM proteins were analyzed. A typical CaM gene, ScCaM1, was subsequently cloned from sugarcane (Saccharum spp. hybrid). Its expression patterns in different tissues and under various abiotic stresses were assessed by quantitative real-time PCR. Then the green fluorescent protein was used to determine the subcellular localization of ScCaM1. Finally, the function of ScCaM1 was evaluated via heterologous yeast expression systems. Results Three typical CaM members (SsCaM1, SsCaM2, and SsCaM3) were identified from the S. spontaneum genome database. CaMs were originated from the two last common ancestors before the origin of angiosperms. The number of CaM family members did not correlate to the genome size but correlated with allopolyploidization events. The ScCaM1 was more highly expressed in buds and roots than in other tissues. The expression patterns of ScCaM1 suggested that it was involved in responses to various abiotic stresses in sugarcane via different hormonal signaling pathways. Noteworthily, its expression levels appeared relatively stable during the cold exposure in the cold-tolerant variety but significantly suppressed in the cold-susceptible variety. Moreover, the recombinant yeast (Pichia pastoris) overexpressing ScCaM1 grew better than the wild-type yeast strain under cold and oxidative stresses. It was revealed that the ScCaM1 played a positive role in reactive oxygen species scavenging and conferred enhanced cold and oxidative stress tolerance to cells. 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Wang, Hengbo |
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Identification of Saccharum CaM gene family and function characterization of ScCaM1 during cold and oxidant exposure in Pichia pastoris Calmodulin (dpeaa)DE-He213 Molecular evolution (dpeaa)DE-He213 Cold stress (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 |
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Identification of Saccharum CaM gene family and function characterization of ScCaM1 during cold and oxidant exposure in Pichia pastoris |
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identification of saccharum cam gene family and function characterization of sccam1 during cold and oxidant exposure in pichia pastoris |
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Identification of Saccharum CaM gene family and function characterization of ScCaM1 during cold and oxidant exposure in Pichia pastoris |
| abstract |
Background Calmodulin (CaM) plays an essential role in binding calcium ions and mediating the interpretation of $ Ca^{2+} $ signals in plants under various stresses. However, the evolutionary relationship of CaM family proteins in Saccharum has not been elucidated. Objective To deduce and explore the evolution and function of Saccharum CaM family. Methods A total of 104 typical CaMs were obtained from Saccharum spontaneum and other 18 plant species. The molecular characteristics and evolution of those CaM proteins were analyzed. A typical CaM gene, ScCaM1, was subsequently cloned from sugarcane (Saccharum spp. hybrid). Its expression patterns in different tissues and under various abiotic stresses were assessed by quantitative real-time PCR. Then the green fluorescent protein was used to determine the subcellular localization of ScCaM1. Finally, the function of ScCaM1 was evaluated via heterologous yeast expression systems. Results Three typical CaM members (SsCaM1, SsCaM2, and SsCaM3) were identified from the S. spontaneum genome database. CaMs were originated from the two last common ancestors before the origin of angiosperms. The number of CaM family members did not correlate to the genome size but correlated with allopolyploidization events. The ScCaM1 was more highly expressed in buds and roots than in other tissues. The expression patterns of ScCaM1 suggested that it was involved in responses to various abiotic stresses in sugarcane via different hormonal signaling pathways. Noteworthily, its expression levels appeared relatively stable during the cold exposure in the cold-tolerant variety but significantly suppressed in the cold-susceptible variety. Moreover, the recombinant yeast (Pichia pastoris) overexpressing ScCaM1 grew better than the wild-type yeast strain under cold and oxidative stresses. It was revealed that the ScCaM1 played a positive role in reactive oxygen species scavenging and conferred enhanced cold and oxidative stress tolerance to cells. Conclusion This study provided comprehensive information on the CaM gene family in Saccharum and would facilitate further investigation of their functional characterization. © The Author(s) under exclusive licence to The Genetics Society of Korea 2022 |
| abstractGer |
Background Calmodulin (CaM) plays an essential role in binding calcium ions and mediating the interpretation of $ Ca^{2+} $ signals in plants under various stresses. However, the evolutionary relationship of CaM family proteins in Saccharum has not been elucidated. Objective To deduce and explore the evolution and function of Saccharum CaM family. Methods A total of 104 typical CaMs were obtained from Saccharum spontaneum and other 18 plant species. The molecular characteristics and evolution of those CaM proteins were analyzed. A typical CaM gene, ScCaM1, was subsequently cloned from sugarcane (Saccharum spp. hybrid). Its expression patterns in different tissues and under various abiotic stresses were assessed by quantitative real-time PCR. Then the green fluorescent protein was used to determine the subcellular localization of ScCaM1. Finally, the function of ScCaM1 was evaluated via heterologous yeast expression systems. Results Three typical CaM members (SsCaM1, SsCaM2, and SsCaM3) were identified from the S. spontaneum genome database. CaMs were originated from the two last common ancestors before the origin of angiosperms. The number of CaM family members did not correlate to the genome size but correlated with allopolyploidization events. The ScCaM1 was more highly expressed in buds and roots than in other tissues. The expression patterns of ScCaM1 suggested that it was involved in responses to various abiotic stresses in sugarcane via different hormonal signaling pathways. Noteworthily, its expression levels appeared relatively stable during the cold exposure in the cold-tolerant variety but significantly suppressed in the cold-susceptible variety. Moreover, the recombinant yeast (Pichia pastoris) overexpressing ScCaM1 grew better than the wild-type yeast strain under cold and oxidative stresses. It was revealed that the ScCaM1 played a positive role in reactive oxygen species scavenging and conferred enhanced cold and oxidative stress tolerance to cells. Conclusion This study provided comprehensive information on the CaM gene family in Saccharum and would facilitate further investigation of their functional characterization. © The Author(s) under exclusive licence to The Genetics Society of Korea 2022 |
| abstract_unstemmed |
Background Calmodulin (CaM) plays an essential role in binding calcium ions and mediating the interpretation of $ Ca^{2+} $ signals in plants under various stresses. However, the evolutionary relationship of CaM family proteins in Saccharum has not been elucidated. Objective To deduce and explore the evolution and function of Saccharum CaM family. Methods A total of 104 typical CaMs were obtained from Saccharum spontaneum and other 18 plant species. The molecular characteristics and evolution of those CaM proteins were analyzed. A typical CaM gene, ScCaM1, was subsequently cloned from sugarcane (Saccharum spp. hybrid). Its expression patterns in different tissues and under various abiotic stresses were assessed by quantitative real-time PCR. Then the green fluorescent protein was used to determine the subcellular localization of ScCaM1. Finally, the function of ScCaM1 was evaluated via heterologous yeast expression systems. Results Three typical CaM members (SsCaM1, SsCaM2, and SsCaM3) were identified from the S. spontaneum genome database. CaMs were originated from the two last common ancestors before the origin of angiosperms. The number of CaM family members did not correlate to the genome size but correlated with allopolyploidization events. The ScCaM1 was more highly expressed in buds and roots than in other tissues. The expression patterns of ScCaM1 suggested that it was involved in responses to various abiotic stresses in sugarcane via different hormonal signaling pathways. Noteworthily, its expression levels appeared relatively stable during the cold exposure in the cold-tolerant variety but significantly suppressed in the cold-susceptible variety. Moreover, the recombinant yeast (Pichia pastoris) overexpressing ScCaM1 grew better than the wild-type yeast strain under cold and oxidative stresses. It was revealed that the ScCaM1 played a positive role in reactive oxygen species scavenging and conferred enhanced cold and oxidative stress tolerance to cells. Conclusion This study provided comprehensive information on the CaM gene family in Saccharum and would facilitate further investigation of their functional characterization. © The Author(s) under exclusive licence to The Genetics Society of Korea 2022 |
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The number of CaM family members did not correlate to the genome size but correlated with allopolyploidization events. The ScCaM1 was more highly expressed in buds and roots than in other tissues. The expression patterns of ScCaM1 suggested that it was involved in responses to various abiotic stresses in sugarcane via different hormonal signaling pathways. Noteworthily, its expression levels appeared relatively stable during the cold exposure in the cold-tolerant variety but significantly suppressed in the cold-susceptible variety. Moreover, the recombinant yeast (Pichia pastoris) overexpressing ScCaM1 grew better than the wild-type yeast strain under cold and oxidative stresses. It was revealed that the ScCaM1 played a positive role in reactive oxygen species scavenging and conferred enhanced cold and oxidative stress tolerance to cells. 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