Urinary HSP70 improves diagnostic accuracy for urinary tract infection in children: UTILISE study

Background The accuracy of conventional urinalysis in diagnosing urinary tract infection (UTI) in children is limited, leading to unnecessary antibiotic exposure in a large fraction of patients. Urinary heat shock protein 70 (uHSP70) is a novel marker of acute urinary tract inflammation. We explored...
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Autor*in:	Yilmaz, Alev [verfasserIn] Afonso, Alberto Caldas Akil, Ipek Aksu, Bagdagul Alpay, Harika Atmis, Bahriye Aydog, Ozlem Bayazıt, Aysun Karabay Bayram, Meral Torun Bilge, Ilmay Bulut, Ipek Kaplan Buyukkaragoz, Bahar Comak, Elif Demir, Belde Kasap Dincel, Nida Donmez, Osman Durmus, Mehmet Akif Dursun, Hasan Dusunsel, Ruhan Duzova, Ali Ertan, Pelin Gedikbasi, Asuman Goknar, Nilufer Guven, Sercin Hacihamdioglu, Duygu Jankauskiene, Augustina Kalyoncu, Mukaddes Kavukcu, Salih Kenan, Bahriye Uzun Kucuk, Nuran Kural, Bahar Litwin, Mieczysław Montini, Giovanni Morello, William Nayir, Ahmet Obrycki, Lukasz Omer, Beyhan Ozdemir, Ebru Misirli Ozkayin, Nese Paripovic, Dusan Pehlivanoglu, Cemile Saygili, Seha Schaefer, Susanne Sonmez, Ferah Tabel, Yilmaz Tas, Nesrin Tasdemir, Mehmet Teixeira, Ana Tekcan, Demet Tulpar, Sebahat Turkkan, Ozde Nisa Uysal, Berfin Uysalol, Metin Vaiciuniene, Daiva Yavuz, Sevgi Yel, Sibel Yildirim, Tarik Yildirim, Zeynep Yuruk Yildiz, Nurdan Yuksel, Selcuk Yurtseven, Eray Schaefer, Franz Topaloglu, Rezan

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Children Urinary tract infection UTI Heat shock proteins HSP70

Anmerkung:	© The Author(s), under exclusive licence to International Pediatric Nephrology Association 2022

Übergeordnetes Werk:	Enthalten in: Pediatric nephrology - Berlin : Springer, 1987, 38(2022), 3 vom: 18. Juli, Seite 791-799
Übergeordnetes Werk:	volume:38 ; year:2022 ; number:3 ; day:18 ; month:07 ; pages:791-799

Links:	Volltext

DOI / URN:	10.1007/s00467-022-05664-5

Katalog-ID:	SPR049065769

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100	1		\|a Yilmaz, Alev \|e verfasserin \|0 (orcid)0000-0003-1743-1491 \|4 aut
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520			\|a Background The accuracy of conventional urinalysis in diagnosing urinary tract infection (UTI) in children is limited, leading to unnecessary antibiotic exposure in a large fraction of patients. Urinary heat shock protein 70 (uHSP70) is a novel marker of acute urinary tract inflammation. We explored the added value of uHSP70 in discriminating UTI from other infections and conditions confused with UTI. Methods A total of 802 children from 37 pediatric centers in seven countries participated in the study. Patients diagnosed with UTI (n = 191), non-UTI infections (n = 178), contaminated urine samples (n = 50), asymptomatic bacteriuria (n = 26), and healthy controls (n = 75) were enrolled. Urine and serum levels of HSP70 were measured at presentation in all patients and after resolution of the infection in patients with confirmed UTI. Results Urinary (u)HSP70 was selectively elevated in children with UTI as compared to all other conditions (p < 0.0001). uHSP70 predicted UTI with 89% sensitivity and 82% specificity (AUC = 0.934). Among the 265 patients with suspected UTI, the uHSP70 > 48 ng/mL criterion identified the 172 children with subsequently confirmed UTI with 90% sensitivity and 82% specificity (AUC = 0.862), exceeding the individual diagnostic accuracy of leukocyturia, nitrite, and leukocyte esterase positivity. uHSP70 had completely normalized by the end of antibiotic therapy in the UTI patients. Serum HSP70 was not predictive. Conclusions Urine HSP70 is a novel non-invasive marker of UTI that improves the diagnostic accuracy of conventional urinalysis. We estimate that rapid urine HSP70 screening could spare empiric antibiotic administration in up to 80% of children with suspected UTI. Graphical abstract A higher resolution version of the Graphical abstract is available as Supplementary information
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912			\|a GBV_ILN_60
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912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
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912			\|a GBV_ILN_636
912			\|a GBV_ILN_702
912			\|a GBV_ILN_711
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2088
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912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2446
912			\|a GBV_ILN_2470
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allfields	10.1007/s00467-022-05664-5 doi (DE-627)SPR049065769 (SPR)s00467-022-05664-5-e DE-627 ger DE-627 rakwb eng Yilmaz, Alev verfasserin (orcid)0000-0003-1743-1491 aut Urinary HSP70 improves diagnostic accuracy for urinary tract infection in children: UTILISE study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to International Pediatric Nephrology Association 2022 Background The accuracy of conventional urinalysis in diagnosing urinary tract infection (UTI) in children is limited, leading to unnecessary antibiotic exposure in a large fraction of patients. Urinary heat shock protein 70 (uHSP70) is a novel marker of acute urinary tract inflammation. We explored the added value of uHSP70 in discriminating UTI from other infections and conditions confused with UTI. Methods A total of 802 children from 37 pediatric centers in seven countries participated in the study. Patients diagnosed with UTI (n = 191), non-UTI infections (n = 178), contaminated urine samples (n = 50), asymptomatic bacteriuria (n = 26), and healthy controls (n = 75) were enrolled. Urine and serum levels of HSP70 were measured at presentation in all patients and after resolution of the infection in patients with confirmed UTI. Results Urinary (u)HSP70 was selectively elevated in children with UTI as compared to all other conditions (p < 0.0001). uHSP70 predicted UTI with 89% sensitivity and 82% specificity (AUC = 0.934). Among the 265 patients with suspected UTI, the uHSP70 > 48 ng/mL criterion identified the 172 children with subsequently confirmed UTI with 90% sensitivity and 82% specificity (AUC = 0.862), exceeding the individual diagnostic accuracy of leukocyturia, nitrite, and leukocyte esterase positivity. uHSP70 had completely normalized by the end of antibiotic therapy in the UTI patients. Serum HSP70 was not predictive. Conclusions Urine HSP70 is a novel non-invasive marker of UTI that improves the diagnostic accuracy of conventional urinalysis. We estimate that rapid urine HSP70 screening could spare empiric antibiotic administration in up to 80% of children with suspected UTI. Graphical abstract A higher resolution version of the Graphical abstract is available as Supplementary information Children (dpeaa)DE-He213 Urinary tract infection (dpeaa)DE-He213 UTI (dpeaa)DE-He213 Heat shock proteins (dpeaa)DE-He213 HSP70 (dpeaa)DE-He213 Afonso, Alberto Caldas aut Akil, Ipek aut Aksu, Bagdagul aut Alpay, Harika aut Atmis, Bahriye aut Aydog, Ozlem aut Bayazıt, Aysun Karabay aut Bayram, Meral Torun aut Bilge, Ilmay aut Bulut, Ipek Kaplan aut Buyukkaragoz, Bahar aut Comak, Elif aut Demir, Belde Kasap aut Dincel, Nida aut Donmez, Osman aut Durmus, Mehmet Akif aut Dursun, Hasan aut Dusunsel, Ruhan aut Duzova, Ali aut Ertan, Pelin aut Gedikbasi, Asuman aut Goknar, Nilufer aut Guven, Sercin aut Hacihamdioglu, Duygu aut Jankauskiene, Augustina aut Kalyoncu, Mukaddes aut Kavukcu, Salih aut Kenan, Bahriye Uzun aut Kucuk, Nuran aut Kural, Bahar aut Litwin, Mieczysław aut Montini, Giovanni aut Morello, William aut Nayir, Ahmet aut Obrycki, Lukasz aut Omer, Beyhan aut Ozdemir, Ebru Misirli aut Ozkayin, Nese aut Paripovic, Dusan aut Pehlivanoglu, Cemile aut Saygili, Seha aut Schaefer, Susanne aut Sonmez, Ferah aut Tabel, Yilmaz aut Tas, Nesrin aut Tasdemir, Mehmet aut Teixeira, Ana aut Tekcan, Demet aut Tulpar, Sebahat aut Turkkan, Ozde Nisa aut Uysal, Berfin aut Uysalol, Metin aut Vaiciuniene, Daiva aut Yavuz, Sevgi aut Yel, Sibel aut Yildirim, Tarik aut Yildirim, Zeynep Yuruk aut Yildiz, Nurdan aut Yuksel, Selcuk aut Yurtseven, Eray aut Schaefer, Franz aut Topaloglu, Rezan aut Enthalten in Pediatric nephrology Berlin : Springer, 1987 38(2022), 3 vom: 18. Juli, Seite 791-799 (DE-627)254638872 (DE-600)1463004-7 1432-198X nnns volume:38 year:2022 number:3 day:18 month:07 pages:791-799 https://dx.doi.org/10.1007/s00467-022-05664-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 38 2022 3 18 07 791-799
spelling	10.1007/s00467-022-05664-5 doi (DE-627)SPR049065769 (SPR)s00467-022-05664-5-e DE-627 ger DE-627 rakwb eng Yilmaz, Alev verfasserin (orcid)0000-0003-1743-1491 aut Urinary HSP70 improves diagnostic accuracy for urinary tract infection in children: UTILISE study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to International Pediatric Nephrology Association 2022 Background The accuracy of conventional urinalysis in diagnosing urinary tract infection (UTI) in children is limited, leading to unnecessary antibiotic exposure in a large fraction of patients. Urinary heat shock protein 70 (uHSP70) is a novel marker of acute urinary tract inflammation. We explored the added value of uHSP70 in discriminating UTI from other infections and conditions confused with UTI. Methods A total of 802 children from 37 pediatric centers in seven countries participated in the study. Patients diagnosed with UTI (n = 191), non-UTI infections (n = 178), contaminated urine samples (n = 50), asymptomatic bacteriuria (n = 26), and healthy controls (n = 75) were enrolled. Urine and serum levels of HSP70 were measured at presentation in all patients and after resolution of the infection in patients with confirmed UTI. Results Urinary (u)HSP70 was selectively elevated in children with UTI as compared to all other conditions (p < 0.0001). uHSP70 predicted UTI with 89% sensitivity and 82% specificity (AUC = 0.934). Among the 265 patients with suspected UTI, the uHSP70 > 48 ng/mL criterion identified the 172 children with subsequently confirmed UTI with 90% sensitivity and 82% specificity (AUC = 0.862), exceeding the individual diagnostic accuracy of leukocyturia, nitrite, and leukocyte esterase positivity. uHSP70 had completely normalized by the end of antibiotic therapy in the UTI patients. Serum HSP70 was not predictive. Conclusions Urine HSP70 is a novel non-invasive marker of UTI that improves the diagnostic accuracy of conventional urinalysis. We estimate that rapid urine HSP70 screening could spare empiric antibiotic administration in up to 80% of children with suspected UTI. Graphical abstract A higher resolution version of the Graphical abstract is available as Supplementary information Children (dpeaa)DE-He213 Urinary tract infection (dpeaa)DE-He213 UTI (dpeaa)DE-He213 Heat shock proteins (dpeaa)DE-He213 HSP70 (dpeaa)DE-He213 Afonso, Alberto Caldas aut Akil, Ipek aut Aksu, Bagdagul aut Alpay, Harika aut Atmis, Bahriye aut Aydog, Ozlem aut Bayazıt, Aysun Karabay aut Bayram, Meral Torun aut Bilge, Ilmay aut Bulut, Ipek Kaplan aut Buyukkaragoz, Bahar aut Comak, Elif aut Demir, Belde Kasap aut Dincel, Nida aut Donmez, Osman aut Durmus, Mehmet Akif aut Dursun, Hasan aut Dusunsel, Ruhan aut Duzova, Ali aut Ertan, Pelin aut Gedikbasi, Asuman aut Goknar, Nilufer aut Guven, Sercin aut Hacihamdioglu, Duygu aut Jankauskiene, Augustina aut Kalyoncu, Mukaddes aut Kavukcu, Salih aut Kenan, Bahriye Uzun aut Kucuk, Nuran aut Kural, Bahar aut Litwin, Mieczysław aut Montini, Giovanni aut Morello, William aut Nayir, Ahmet aut Obrycki, Lukasz aut Omer, Beyhan aut Ozdemir, Ebru Misirli aut Ozkayin, Nese aut Paripovic, Dusan aut Pehlivanoglu, Cemile aut Saygili, Seha aut Schaefer, Susanne aut Sonmez, Ferah aut Tabel, Yilmaz aut Tas, Nesrin aut Tasdemir, Mehmet aut Teixeira, Ana aut Tekcan, Demet aut Tulpar, Sebahat aut Turkkan, Ozde Nisa aut Uysal, Berfin aut Uysalol, Metin aut Vaiciuniene, Daiva aut Yavuz, Sevgi aut Yel, Sibel aut Yildirim, Tarik aut Yildirim, Zeynep Yuruk aut Yildiz, Nurdan aut Yuksel, Selcuk aut Yurtseven, Eray aut Schaefer, Franz aut Topaloglu, Rezan aut Enthalten in Pediatric nephrology Berlin : Springer, 1987 38(2022), 3 vom: 18. Juli, Seite 791-799 (DE-627)254638872 (DE-600)1463004-7 1432-198X nnns volume:38 year:2022 number:3 day:18 month:07 pages:791-799 https://dx.doi.org/10.1007/s00467-022-05664-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 38 2022 3 18 07 791-799
allfields_unstemmed	10.1007/s00467-022-05664-5 doi (DE-627)SPR049065769 (SPR)s00467-022-05664-5-e DE-627 ger DE-627 rakwb eng Yilmaz, Alev verfasserin (orcid)0000-0003-1743-1491 aut Urinary HSP70 improves diagnostic accuracy for urinary tract infection in children: UTILISE study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to International Pediatric Nephrology Association 2022 Background The accuracy of conventional urinalysis in diagnosing urinary tract infection (UTI) in children is limited, leading to unnecessary antibiotic exposure in a large fraction of patients. Urinary heat shock protein 70 (uHSP70) is a novel marker of acute urinary tract inflammation. We explored the added value of uHSP70 in discriminating UTI from other infections and conditions confused with UTI. Methods A total of 802 children from 37 pediatric centers in seven countries participated in the study. Patients diagnosed with UTI (n = 191), non-UTI infections (n = 178), contaminated urine samples (n = 50), asymptomatic bacteriuria (n = 26), and healthy controls (n = 75) were enrolled. Urine and serum levels of HSP70 were measured at presentation in all patients and after resolution of the infection in patients with confirmed UTI. Results Urinary (u)HSP70 was selectively elevated in children with UTI as compared to all other conditions (p < 0.0001). uHSP70 predicted UTI with 89% sensitivity and 82% specificity (AUC = 0.934). Among the 265 patients with suspected UTI, the uHSP70 > 48 ng/mL criterion identified the 172 children with subsequently confirmed UTI with 90% sensitivity and 82% specificity (AUC = 0.862), exceeding the individual diagnostic accuracy of leukocyturia, nitrite, and leukocyte esterase positivity. uHSP70 had completely normalized by the end of antibiotic therapy in the UTI patients. Serum HSP70 was not predictive. Conclusions Urine HSP70 is a novel non-invasive marker of UTI that improves the diagnostic accuracy of conventional urinalysis. We estimate that rapid urine HSP70 screening could spare empiric antibiotic administration in up to 80% of children with suspected UTI. Graphical abstract A higher resolution version of the Graphical abstract is available as Supplementary information Children (dpeaa)DE-He213 Urinary tract infection (dpeaa)DE-He213 UTI (dpeaa)DE-He213 Heat shock proteins (dpeaa)DE-He213 HSP70 (dpeaa)DE-He213 Afonso, Alberto Caldas aut Akil, Ipek aut Aksu, Bagdagul aut Alpay, Harika aut Atmis, Bahriye aut Aydog, Ozlem aut Bayazıt, Aysun Karabay aut Bayram, Meral Torun aut Bilge, Ilmay aut Bulut, Ipek Kaplan aut Buyukkaragoz, Bahar aut Comak, Elif aut Demir, Belde Kasap aut Dincel, Nida aut Donmez, Osman aut Durmus, Mehmet Akif aut Dursun, Hasan aut Dusunsel, Ruhan aut Duzova, Ali aut Ertan, Pelin aut Gedikbasi, Asuman aut Goknar, Nilufer aut Guven, Sercin aut Hacihamdioglu, Duygu aut Jankauskiene, Augustina aut Kalyoncu, Mukaddes aut Kavukcu, Salih aut Kenan, Bahriye Uzun aut Kucuk, Nuran aut Kural, Bahar aut Litwin, Mieczysław aut Montini, Giovanni aut Morello, William aut Nayir, Ahmet aut Obrycki, Lukasz aut Omer, Beyhan aut Ozdemir, Ebru Misirli aut Ozkayin, Nese aut Paripovic, Dusan aut Pehlivanoglu, Cemile aut Saygili, Seha aut Schaefer, Susanne aut Sonmez, Ferah aut Tabel, Yilmaz aut Tas, Nesrin aut Tasdemir, Mehmet aut Teixeira, Ana aut Tekcan, Demet aut Tulpar, Sebahat aut Turkkan, Ozde Nisa aut Uysal, Berfin aut Uysalol, Metin aut Vaiciuniene, Daiva aut Yavuz, Sevgi aut Yel, Sibel aut Yildirim, Tarik aut Yildirim, Zeynep Yuruk aut Yildiz, Nurdan aut Yuksel, Selcuk aut Yurtseven, Eray aut Schaefer, Franz aut Topaloglu, Rezan aut Enthalten in Pediatric nephrology Berlin : Springer, 1987 38(2022), 3 vom: 18. Juli, Seite 791-799 (DE-627)254638872 (DE-600)1463004-7 1432-198X nnns volume:38 year:2022 number:3 day:18 month:07 pages:791-799 https://dx.doi.org/10.1007/s00467-022-05664-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 38 2022 3 18 07 791-799
allfieldsGer	10.1007/s00467-022-05664-5 doi (DE-627)SPR049065769 (SPR)s00467-022-05664-5-e DE-627 ger DE-627 rakwb eng Yilmaz, Alev verfasserin (orcid)0000-0003-1743-1491 aut Urinary HSP70 improves diagnostic accuracy for urinary tract infection in children: UTILISE study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to International Pediatric Nephrology Association 2022 Background The accuracy of conventional urinalysis in diagnosing urinary tract infection (UTI) in children is limited, leading to unnecessary antibiotic exposure in a large fraction of patients. Urinary heat shock protein 70 (uHSP70) is a novel marker of acute urinary tract inflammation. We explored the added value of uHSP70 in discriminating UTI from other infections and conditions confused with UTI. Methods A total of 802 children from 37 pediatric centers in seven countries participated in the study. Patients diagnosed with UTI (n = 191), non-UTI infections (n = 178), contaminated urine samples (n = 50), asymptomatic bacteriuria (n = 26), and healthy controls (n = 75) were enrolled. Urine and serum levels of HSP70 were measured at presentation in all patients and after resolution of the infection in patients with confirmed UTI. Results Urinary (u)HSP70 was selectively elevated in children with UTI as compared to all other conditions (p < 0.0001). uHSP70 predicted UTI with 89% sensitivity and 82% specificity (AUC = 0.934). Among the 265 patients with suspected UTI, the uHSP70 > 48 ng/mL criterion identified the 172 children with subsequently confirmed UTI with 90% sensitivity and 82% specificity (AUC = 0.862), exceeding the individual diagnostic accuracy of leukocyturia, nitrite, and leukocyte esterase positivity. uHSP70 had completely normalized by the end of antibiotic therapy in the UTI patients. Serum HSP70 was not predictive. Conclusions Urine HSP70 is a novel non-invasive marker of UTI that improves the diagnostic accuracy of conventional urinalysis. We estimate that rapid urine HSP70 screening could spare empiric antibiotic administration in up to 80% of children with suspected UTI. Graphical abstract A higher resolution version of the Graphical abstract is available as Supplementary information Children (dpeaa)DE-He213 Urinary tract infection (dpeaa)DE-He213 UTI (dpeaa)DE-He213 Heat shock proteins (dpeaa)DE-He213 HSP70 (dpeaa)DE-He213 Afonso, Alberto Caldas aut Akil, Ipek aut Aksu, Bagdagul aut Alpay, Harika aut Atmis, Bahriye aut Aydog, Ozlem aut Bayazıt, Aysun Karabay aut Bayram, Meral Torun aut Bilge, Ilmay aut Bulut, Ipek Kaplan aut Buyukkaragoz, Bahar aut Comak, Elif aut Demir, Belde Kasap aut Dincel, Nida aut Donmez, Osman aut Durmus, Mehmet Akif aut Dursun, Hasan aut Dusunsel, Ruhan aut Duzova, Ali aut Ertan, Pelin aut Gedikbasi, Asuman aut Goknar, Nilufer aut Guven, Sercin aut Hacihamdioglu, Duygu aut Jankauskiene, Augustina aut Kalyoncu, Mukaddes aut Kavukcu, Salih aut Kenan, Bahriye Uzun aut Kucuk, Nuran aut Kural, Bahar aut Litwin, Mieczysław aut Montini, Giovanni aut Morello, William aut Nayir, Ahmet aut Obrycki, Lukasz aut Omer, Beyhan aut Ozdemir, Ebru Misirli aut Ozkayin, Nese aut Paripovic, Dusan aut Pehlivanoglu, Cemile aut Saygili, Seha aut Schaefer, Susanne aut Sonmez, Ferah aut Tabel, Yilmaz aut Tas, Nesrin aut Tasdemir, Mehmet aut Teixeira, Ana aut Tekcan, Demet aut Tulpar, Sebahat aut Turkkan, Ozde Nisa aut Uysal, Berfin aut Uysalol, Metin aut Vaiciuniene, Daiva aut Yavuz, Sevgi aut Yel, Sibel aut Yildirim, Tarik aut Yildirim, Zeynep Yuruk aut Yildiz, Nurdan aut Yuksel, Selcuk aut Yurtseven, Eray aut Schaefer, Franz aut Topaloglu, Rezan aut Enthalten in Pediatric nephrology Berlin : Springer, 1987 38(2022), 3 vom: 18. Juli, Seite 791-799 (DE-627)254638872 (DE-600)1463004-7 1432-198X nnns volume:38 year:2022 number:3 day:18 month:07 pages:791-799 https://dx.doi.org/10.1007/s00467-022-05664-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 38 2022 3 18 07 791-799
allfieldsSound	10.1007/s00467-022-05664-5 doi (DE-627)SPR049065769 (SPR)s00467-022-05664-5-e DE-627 ger DE-627 rakwb eng Yilmaz, Alev verfasserin (orcid)0000-0003-1743-1491 aut Urinary HSP70 improves diagnostic accuracy for urinary tract infection in children: UTILISE study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to International Pediatric Nephrology Association 2022 Background The accuracy of conventional urinalysis in diagnosing urinary tract infection (UTI) in children is limited, leading to unnecessary antibiotic exposure in a large fraction of patients. Urinary heat shock protein 70 (uHSP70) is a novel marker of acute urinary tract inflammation. We explored the added value of uHSP70 in discriminating UTI from other infections and conditions confused with UTI. Methods A total of 802 children from 37 pediatric centers in seven countries participated in the study. Patients diagnosed with UTI (n = 191), non-UTI infections (n = 178), contaminated urine samples (n = 50), asymptomatic bacteriuria (n = 26), and healthy controls (n = 75) were enrolled. Urine and serum levels of HSP70 were measured at presentation in all patients and after resolution of the infection in patients with confirmed UTI. Results Urinary (u)HSP70 was selectively elevated in children with UTI as compared to all other conditions (p < 0.0001). uHSP70 predicted UTI with 89% sensitivity and 82% specificity (AUC = 0.934). Among the 265 patients with suspected UTI, the uHSP70 > 48 ng/mL criterion identified the 172 children with subsequently confirmed UTI with 90% sensitivity and 82% specificity (AUC = 0.862), exceeding the individual diagnostic accuracy of leukocyturia, nitrite, and leukocyte esterase positivity. uHSP70 had completely normalized by the end of antibiotic therapy in the UTI patients. Serum HSP70 was not predictive. Conclusions Urine HSP70 is a novel non-invasive marker of UTI that improves the diagnostic accuracy of conventional urinalysis. We estimate that rapid urine HSP70 screening could spare empiric antibiotic administration in up to 80% of children with suspected UTI. Graphical abstract A higher resolution version of the Graphical abstract is available as Supplementary information Children (dpeaa)DE-He213 Urinary tract infection (dpeaa)DE-He213 UTI (dpeaa)DE-He213 Heat shock proteins (dpeaa)DE-He213 HSP70 (dpeaa)DE-He213 Afonso, Alberto Caldas aut Akil, Ipek aut Aksu, Bagdagul aut Alpay, Harika aut Atmis, Bahriye aut Aydog, Ozlem aut Bayazıt, Aysun Karabay aut Bayram, Meral Torun aut Bilge, Ilmay aut Bulut, Ipek Kaplan aut Buyukkaragoz, Bahar aut Comak, Elif aut Demir, Belde Kasap aut Dincel, Nida aut Donmez, Osman aut Durmus, Mehmet Akif aut Dursun, Hasan aut Dusunsel, Ruhan aut Duzova, Ali aut Ertan, Pelin aut Gedikbasi, Asuman aut Goknar, Nilufer aut Guven, Sercin aut Hacihamdioglu, Duygu aut Jankauskiene, Augustina aut Kalyoncu, Mukaddes aut Kavukcu, Salih aut Kenan, Bahriye Uzun aut Kucuk, Nuran aut Kural, Bahar aut Litwin, Mieczysław aut Montini, Giovanni aut Morello, William aut Nayir, Ahmet aut Obrycki, Lukasz aut Omer, Beyhan aut Ozdemir, Ebru Misirli aut Ozkayin, Nese aut Paripovic, Dusan aut Pehlivanoglu, Cemile aut Saygili, Seha aut Schaefer, Susanne aut Sonmez, Ferah aut Tabel, Yilmaz aut Tas, Nesrin aut Tasdemir, Mehmet aut Teixeira, Ana aut Tekcan, Demet aut Tulpar, Sebahat aut Turkkan, Ozde Nisa aut Uysal, Berfin aut Uysalol, Metin aut Vaiciuniene, Daiva aut Yavuz, Sevgi aut Yel, Sibel aut Yildirim, Tarik aut Yildirim, Zeynep Yuruk aut Yildiz, Nurdan aut Yuksel, Selcuk aut Yurtseven, Eray aut Schaefer, Franz aut Topaloglu, Rezan aut Enthalten in Pediatric nephrology Berlin : Springer, 1987 38(2022), 3 vom: 18. Juli, Seite 791-799 (DE-627)254638872 (DE-600)1463004-7 1432-198X nnns volume:38 year:2022 number:3 day:18 month:07 pages:791-799 https://dx.doi.org/10.1007/s00467-022-05664-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 38 2022 3 18 07 791-799
language	English
source	Enthalten in Pediatric nephrology 38(2022), 3 vom: 18. Juli, Seite 791-799 volume:38 year:2022 number:3 day:18 month:07 pages:791-799
sourceStr	Enthalten in Pediatric nephrology 38(2022), 3 vom: 18. Juli, Seite 791-799 volume:38 year:2022 number:3 day:18 month:07 pages:791-799
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Children Urinary tract infection UTI Heat shock proteins HSP70
isfreeaccess_bool	false
container_title	Pediatric nephrology
authorswithroles_txt_mv	Yilmaz, Alev @@aut@@ Afonso, Alberto Caldas @@aut@@ Akil, Ipek @@aut@@ Aksu, Bagdagul @@aut@@ Alpay, Harika @@aut@@ Atmis, Bahriye @@aut@@ Aydog, Ozlem @@aut@@ Bayazıt, Aysun Karabay @@aut@@ Bayram, Meral Torun @@aut@@ Bilge, Ilmay @@aut@@ Bulut, Ipek Kaplan @@aut@@ Buyukkaragoz, Bahar @@aut@@ Comak, Elif @@aut@@ Demir, Belde Kasap @@aut@@ Dincel, Nida @@aut@@ Donmez, Osman @@aut@@ Durmus, Mehmet Akif @@aut@@ Dursun, Hasan @@aut@@ Dusunsel, Ruhan @@aut@@ Duzova, Ali @@aut@@ Ertan, Pelin @@aut@@ Gedikbasi, Asuman @@aut@@ Goknar, Nilufer @@aut@@ Guven, Sercin @@aut@@ Hacihamdioglu, Duygu @@aut@@ Jankauskiene, Augustina @@aut@@ Kalyoncu, Mukaddes @@aut@@ Kavukcu, Salih @@aut@@ Kenan, Bahriye Uzun @@aut@@ Kucuk, Nuran @@aut@@ Kural, Bahar @@aut@@ Litwin, Mieczysław @@aut@@ Montini, Giovanni @@aut@@ Morello, William @@aut@@ Nayir, Ahmet @@aut@@ Obrycki, Lukasz @@aut@@ Omer, Beyhan @@aut@@ Ozdemir, Ebru Misirli @@aut@@ Ozkayin, Nese @@aut@@ Paripovic, Dusan @@aut@@ Pehlivanoglu, Cemile @@aut@@ Saygili, Seha @@aut@@ Schaefer, Susanne @@aut@@ Sonmez, Ferah @@aut@@ Tabel, Yilmaz @@aut@@ Tas, Nesrin @@aut@@ Tasdemir, Mehmet @@aut@@ Teixeira, Ana @@aut@@ Tekcan, Demet @@aut@@ Tulpar, Sebahat @@aut@@ Turkkan, Ozde Nisa @@aut@@ Uysal, Berfin @@aut@@ Uysalol, Metin @@aut@@ Vaiciuniene, Daiva @@aut@@ Yavuz, Sevgi @@aut@@ Yel, Sibel @@aut@@ Yildirim, Tarik @@aut@@ Yildirim, Zeynep Yuruk @@aut@@ Yildiz, Nurdan @@aut@@ Yuksel, Selcuk @@aut@@ Yurtseven, Eray @@aut@@ Schaefer, Franz @@aut@@ Topaloglu, Rezan @@aut@@
publishDateDaySort_date	2022-07-18T00:00:00Z
hierarchy_top_id	254638872
id	SPR049065769
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR049065769</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519163157.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230117s2022 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00467-022-05664-5</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR049065769</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s00467-022-05664-5-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Yilmaz, Alev</subfield><subfield code="e">verfasserin</subfield><subfield code="0">(orcid)0000-0003-1743-1491</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Urinary HSP70 improves diagnostic accuracy for urinary tract infection in children: UTILISE study</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2022</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© The Author(s), under exclusive licence to International Pediatric Nephrology Association 2022</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Background The accuracy of conventional urinalysis in diagnosing urinary tract infection (UTI) in children is limited, leading to unnecessary antibiotic exposure in a large fraction of patients. Urinary heat shock protein 70 (uHSP70) is a novel marker of acute urinary tract inflammation. We explored the added value of uHSP70 in discriminating UTI from other infections and conditions confused with UTI. Methods A total of 802 children from 37 pediatric centers in seven countries participated in the study. Patients diagnosed with UTI (n = 191), non-UTI infections (n = 178), contaminated urine samples (n = 50), asymptomatic bacteriuria (n = 26), and healthy controls (n = 75) were enrolled. Urine and serum levels of HSP70 were measured at presentation in all patients and after resolution of the infection in patients with confirmed UTI. Results Urinary (u)HSP70 was selectively elevated in children with UTI as compared to all other conditions (p < 0.0001). uHSP70 predicted UTI with 89% sensitivity and 82% specificity (AUC = 0.934). Among the 265 patients with suspected UTI, the uHSP70 > 48 ng/mL criterion identified the 172 children with subsequently confirmed UTI with 90% sensitivity and 82% specificity (AUC = 0.862), exceeding the individual diagnostic accuracy of leukocyturia, nitrite, and leukocyte esterase positivity. uHSP70 had completely normalized by the end of antibiotic therapy in the UTI patients. Serum HSP70 was not predictive. Conclusions Urine HSP70 is a novel non-invasive marker of UTI that improves the diagnostic accuracy of conventional urinalysis. We estimate that rapid urine HSP70 screening could spare empiric antibiotic administration in up to 80% of children with suspected UTI. 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author	Yilmaz, Alev
spellingShingle	Yilmaz, Alev misc Children misc Urinary tract infection misc UTI misc Heat shock proteins misc HSP70 Urinary HSP70 improves diagnostic accuracy for urinary tract infection in children: UTILISE study
authorStr	Yilmaz, Alev
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collection	springer
remote_str	true
illustrated	Not Illustrated
issn	1432-198X
topic_title	Urinary HSP70 improves diagnostic accuracy for urinary tract infection in children: UTILISE study Children (dpeaa)DE-He213 Urinary tract infection (dpeaa)DE-He213 UTI (dpeaa)DE-He213 Heat shock proteins (dpeaa)DE-He213 HSP70 (dpeaa)DE-He213
topic	misc Children misc Urinary tract infection misc UTI misc Heat shock proteins misc HSP70
topic_unstemmed	misc Children misc Urinary tract infection misc UTI misc Heat shock proteins misc HSP70
topic_browse	misc Children misc Urinary tract infection misc UTI misc Heat shock proteins misc HSP70
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
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hierarchy_parent_title	Pediatric nephrology
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hierarchy_top_title	Pediatric nephrology
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title	Urinary HSP70 improves diagnostic accuracy for urinary tract infection in children: UTILISE study
ctrlnum	(DE-627)SPR049065769 (SPR)s00467-022-05664-5-e
title_full	Urinary HSP70 improves diagnostic accuracy for urinary tract infection in children: UTILISE study
author_sort	Yilmaz, Alev
journal	Pediatric nephrology
journalStr	Pediatric nephrology
lang_code	eng
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publishDateSort	2022
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container_start_page	791
author_browse	Yilmaz, Alev Afonso, Alberto Caldas Akil, Ipek Aksu, Bagdagul Alpay, Harika Atmis, Bahriye Aydog, Ozlem Bayazıt, Aysun Karabay Bayram, Meral Torun Bilge, Ilmay Bulut, Ipek Kaplan Buyukkaragoz, Bahar Comak, Elif Demir, Belde Kasap Dincel, Nida Donmez, Osman Durmus, Mehmet Akif Dursun, Hasan Dusunsel, Ruhan Duzova, Ali Ertan, Pelin Gedikbasi, Asuman Goknar, Nilufer Guven, Sercin Hacihamdioglu, Duygu Jankauskiene, Augustina Kalyoncu, Mukaddes Kavukcu, Salih Kenan, Bahriye Uzun Kucuk, Nuran Kural, Bahar Litwin, Mieczysław Montini, Giovanni Morello, William Nayir, Ahmet Obrycki, Lukasz Omer, Beyhan Ozdemir, Ebru Misirli Ozkayin, Nese Paripovic, Dusan Pehlivanoglu, Cemile Saygili, Seha Schaefer, Susanne Sonmez, Ferah Tabel, Yilmaz Tas, Nesrin Tasdemir, Mehmet Teixeira, Ana Tekcan, Demet Tulpar, Sebahat Turkkan, Ozde Nisa Uysal, Berfin Uysalol, Metin Vaiciuniene, Daiva Yavuz, Sevgi Yel, Sibel Yildirim, Tarik Yildirim, Zeynep Yuruk Yildiz, Nurdan Yuksel, Selcuk Yurtseven, Eray Schaefer, Franz Topaloglu, Rezan
container_volume	38
format_se	Elektronische Aufsätze
author-letter	Yilmaz, Alev
doi_str_mv	10.1007/s00467-022-05664-5
normlink	(ORCID)0000-0003-1743-1491
normlink_prefix_str_mv	(orcid)0000-0003-1743-1491
title_sort	urinary hsp70 improves diagnostic accuracy for urinary tract infection in children: utilise study
title_auth	Urinary HSP70 improves diagnostic accuracy for urinary tract infection in children: UTILISE study
abstract	Background The accuracy of conventional urinalysis in diagnosing urinary tract infection (UTI) in children is limited, leading to unnecessary antibiotic exposure in a large fraction of patients. Urinary heat shock protein 70 (uHSP70) is a novel marker of acute urinary tract inflammation. We explored the added value of uHSP70 in discriminating UTI from other infections and conditions confused with UTI. Methods A total of 802 children from 37 pediatric centers in seven countries participated in the study. Patients diagnosed with UTI (n = 191), non-UTI infections (n = 178), contaminated urine samples (n = 50), asymptomatic bacteriuria (n = 26), and healthy controls (n = 75) were enrolled. Urine and serum levels of HSP70 were measured at presentation in all patients and after resolution of the infection in patients with confirmed UTI. Results Urinary (u)HSP70 was selectively elevated in children with UTI as compared to all other conditions (p < 0.0001). uHSP70 predicted UTI with 89% sensitivity and 82% specificity (AUC = 0.934). Among the 265 patients with suspected UTI, the uHSP70 > 48 ng/mL criterion identified the 172 children with subsequently confirmed UTI with 90% sensitivity and 82% specificity (AUC = 0.862), exceeding the individual diagnostic accuracy of leukocyturia, nitrite, and leukocyte esterase positivity. uHSP70 had completely normalized by the end of antibiotic therapy in the UTI patients. Serum HSP70 was not predictive. Conclusions Urine HSP70 is a novel non-invasive marker of UTI that improves the diagnostic accuracy of conventional urinalysis. We estimate that rapid urine HSP70 screening could spare empiric antibiotic administration in up to 80% of children with suspected UTI. Graphical abstract A higher resolution version of the Graphical abstract is available as Supplementary information © The Author(s), under exclusive licence to International Pediatric Nephrology Association 2022
abstractGer	Background The accuracy of conventional urinalysis in diagnosing urinary tract infection (UTI) in children is limited, leading to unnecessary antibiotic exposure in a large fraction of patients. Urinary heat shock protein 70 (uHSP70) is a novel marker of acute urinary tract inflammation. We explored the added value of uHSP70 in discriminating UTI from other infections and conditions confused with UTI. Methods A total of 802 children from 37 pediatric centers in seven countries participated in the study. Patients diagnosed with UTI (n = 191), non-UTI infections (n = 178), contaminated urine samples (n = 50), asymptomatic bacteriuria (n = 26), and healthy controls (n = 75) were enrolled. Urine and serum levels of HSP70 were measured at presentation in all patients and after resolution of the infection in patients with confirmed UTI. Results Urinary (u)HSP70 was selectively elevated in children with UTI as compared to all other conditions (p < 0.0001). uHSP70 predicted UTI with 89% sensitivity and 82% specificity (AUC = 0.934). Among the 265 patients with suspected UTI, the uHSP70 > 48 ng/mL criterion identified the 172 children with subsequently confirmed UTI with 90% sensitivity and 82% specificity (AUC = 0.862), exceeding the individual diagnostic accuracy of leukocyturia, nitrite, and leukocyte esterase positivity. uHSP70 had completely normalized by the end of antibiotic therapy in the UTI patients. Serum HSP70 was not predictive. Conclusions Urine HSP70 is a novel non-invasive marker of UTI that improves the diagnostic accuracy of conventional urinalysis. We estimate that rapid urine HSP70 screening could spare empiric antibiotic administration in up to 80% of children with suspected UTI. Graphical abstract A higher resolution version of the Graphical abstract is available as Supplementary information © The Author(s), under exclusive licence to International Pediatric Nephrology Association 2022
abstract_unstemmed	Background The accuracy of conventional urinalysis in diagnosing urinary tract infection (UTI) in children is limited, leading to unnecessary antibiotic exposure in a large fraction of patients. Urinary heat shock protein 70 (uHSP70) is a novel marker of acute urinary tract inflammation. We explored the added value of uHSP70 in discriminating UTI from other infections and conditions confused with UTI. Methods A total of 802 children from 37 pediatric centers in seven countries participated in the study. Patients diagnosed with UTI (n = 191), non-UTI infections (n = 178), contaminated urine samples (n = 50), asymptomatic bacteriuria (n = 26), and healthy controls (n = 75) were enrolled. Urine and serum levels of HSP70 were measured at presentation in all patients and after resolution of the infection in patients with confirmed UTI. Results Urinary (u)HSP70 was selectively elevated in children with UTI as compared to all other conditions (p < 0.0001). uHSP70 predicted UTI with 89% sensitivity and 82% specificity (AUC = 0.934). Among the 265 patients with suspected UTI, the uHSP70 > 48 ng/mL criterion identified the 172 children with subsequently confirmed UTI with 90% sensitivity and 82% specificity (AUC = 0.862), exceeding the individual diagnostic accuracy of leukocyturia, nitrite, and leukocyte esterase positivity. uHSP70 had completely normalized by the end of antibiotic therapy in the UTI patients. Serum HSP70 was not predictive. Conclusions Urine HSP70 is a novel non-invasive marker of UTI that improves the diagnostic accuracy of conventional urinalysis. We estimate that rapid urine HSP70 screening could spare empiric antibiotic administration in up to 80% of children with suspected UTI. Graphical abstract A higher resolution version of the Graphical abstract is available as Supplementary information © The Author(s), under exclusive licence to International Pediatric Nephrology Association 2022
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title_short	Urinary HSP70 improves diagnostic accuracy for urinary tract infection in children: UTILISE study
url	https://dx.doi.org/10.1007/s00467-022-05664-5
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author2	Afonso, Alberto Caldas Akil, Ipek Aksu, Bagdagul Alpay, Harika Atmis, Bahriye Aydog, Ozlem Bayazıt, Aysun Karabay Bayram, Meral Torun Bilge, Ilmay Bulut, Ipek Kaplan Buyukkaragoz, Bahar Comak, Elif Demir, Belde Kasap Dincel, Nida Donmez, Osman Durmus, Mehmet Akif Dursun, Hasan Dusunsel, Ruhan Duzova, Ali Ertan, Pelin Gedikbasi, Asuman Goknar, Nilufer Guven, Sercin Hacihamdioglu, Duygu Jankauskiene, Augustina Kalyoncu, Mukaddes Kavukcu, Salih Kenan, Bahriye Uzun Kucuk, Nuran Kural, Bahar Litwin, Mieczysław Montini, Giovanni Morello, William Nayir, Ahmet Obrycki, Lukasz Omer, Beyhan Ozdemir, Ebru Misirli Ozkayin, Nese Paripovic, Dusan Pehlivanoglu, Cemile Saygili, Seha Schaefer, Susanne Sonmez, Ferah Tabel, Yilmaz Tas, Nesrin Tasdemir, Mehmet Teixeira, Ana Tekcan, Demet Tulpar, Sebahat Turkkan, Ozde Nisa Uysal, Berfin Uysalol, Metin Vaiciuniene, Daiva Yavuz, Sevgi Yel, Sibel Yildirim, Tarik Yildirim, Zeynep Yuruk Yildiz, Nurdan Yuksel, Selcuk Yurtseven, Eray Schaefer, Franz Topaloglu, Rezan
author2Str	Afonso, Alberto Caldas Akil, Ipek Aksu, Bagdagul Alpay, Harika Atmis, Bahriye Aydog, Ozlem Bayazıt, Aysun Karabay Bayram, Meral Torun Bilge, Ilmay Bulut, Ipek Kaplan Buyukkaragoz, Bahar Comak, Elif Demir, Belde Kasap Dincel, Nida Donmez, Osman Durmus, Mehmet Akif Dursun, Hasan Dusunsel, Ruhan Duzova, Ali Ertan, Pelin Gedikbasi, Asuman Goknar, Nilufer Guven, Sercin Hacihamdioglu, Duygu Jankauskiene, Augustina Kalyoncu, Mukaddes Kavukcu, Salih Kenan, Bahriye Uzun Kucuk, Nuran Kural, Bahar Litwin, Mieczysław Montini, Giovanni Morello, William Nayir, Ahmet Obrycki, Lukasz Omer, Beyhan Ozdemir, Ebru Misirli Ozkayin, Nese Paripovic, Dusan Pehlivanoglu, Cemile Saygili, Seha Schaefer, Susanne Sonmez, Ferah Tabel, Yilmaz Tas, Nesrin Tasdemir, Mehmet Teixeira, Ana Tekcan, Demet Tulpar, Sebahat Turkkan, Ozde Nisa Uysal, Berfin Uysalol, Metin Vaiciuniene, Daiva Yavuz, Sevgi Yel, Sibel Yildirim, Tarik Yildirim, Zeynep Yuruk Yildiz, Nurdan Yuksel, Selcuk Yurtseven, Eray Schaefer, Franz Topaloglu, Rezan
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doi_str	10.1007/s00467-022-05664-5
up_date	2024-07-03T23:07:04.653Z
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Urinary heat shock protein 70 (uHSP70) is a novel marker of acute urinary tract inflammation. We explored the added value of uHSP70 in discriminating UTI from other infections and conditions confused with UTI. Methods A total of 802 children from 37 pediatric centers in seven countries participated in the study. Patients diagnosed with UTI (n = 191), non-UTI infections (n = 178), contaminated urine samples (n = 50), asymptomatic bacteriuria (n = 26), and healthy controls (n = 75) were enrolled. Urine and serum levels of HSP70 were measured at presentation in all patients and after resolution of the infection in patients with confirmed UTI. Results Urinary (u)HSP70 was selectively elevated in children with UTI as compared to all other conditions (p < 0.0001). uHSP70 predicted UTI with 89% sensitivity and 82% specificity (AUC = 0.934). Among the 265 patients with suspected UTI, the uHSP70 > 48 ng/mL criterion identified the 172 children with subsequently confirmed UTI with 90% sensitivity and 82% specificity (AUC = 0.862), exceeding the individual diagnostic accuracy of leukocyturia, nitrite, and leukocyte esterase positivity. uHSP70 had completely normalized by the end of antibiotic therapy in the UTI patients. Serum HSP70 was not predictive. Conclusions Urine HSP70 is a novel non-invasive marker of UTI that improves the diagnostic accuracy of conventional urinalysis. We estimate that rapid urine HSP70 screening could spare empiric antibiotic administration in up to 80% of children with suspected UTI. 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