Water restriction technique for inoculation of Fusarium meridionale in wheat seeds
Abstract Fusarium head blight (FHB) is a disease of wheat caused by the Fusarium graminearum species complex in which Fusarium meridionale is included. The seed is an inoculum source and the use of inoculated seeds for this pathosystem studies can optimize the time of experimental tests. However ear...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
de Macedo Nava, Francine Falcão [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2023 |
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| Schlagwörter: |
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| Anmerkung: |
© Koninklijke Nederlandse Planteziektenkundige Vereniging 2023 |
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| Übergeordnetes Werk: |
Enthalten in: European journal of plant pathology - Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895, 165(2023), 3 vom: 03. Jan., Seite 603-608 |
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| Übergeordnetes Werk: |
volume:165 ; year:2023 ; number:3 ; day:03 ; month:01 ; pages:603-608 |
| Links: |
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| DOI / URN: |
10.1007/s10658-022-02621-8 |
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| Katalog-ID: |
SPR049319108 |
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| 520 | |a Abstract Fusarium head blight (FHB) is a disease of wheat caused by the Fusarium graminearum species complex in which Fusarium meridionale is included. The seed is an inoculum source and the use of inoculated seeds for this pathosystem studies can optimize the time of experimental tests. However early germination of seed during the inoculation process can make studies on the FHB pathosystem very challenging. Therefore, this study aimed to determine the optimal water restriction condition and time of exposure for inoculation of F. meridionale in wheat seeds without reducing seed germination, to define a reliable and rapid technique for inoculating FHB using wheat seeds. Mycelial discs of F. meridionale were placed in the center of Petri dishes containing potato dextrose agar (PDA) media with different quantities of mannitol to establish water potentials of −0.4, −0.6, −0.8, −1.0 or − 1.2 MPa. A control plate with only PDA had −0.36 MPa. Mycelial growth was measured for 178 hours post inoculation (hpi). Wheat seeds were placed in contact with the mycelium at 12, 24, 36, 48 or 60 hpi. Seed germination and seed pathology were evaluated. The greatest growth of the pathogen was at water potentials between −0.8 and − 1.06 MPa. Seed germination was not affected by the water potential at 12 hpi. It was verified that at −1.0 MPa the seeds did not germinate early, and the pathogen was detected in 100% of the inoculated seeds. Thus, the use of mannitol at water potential of −1.0 MPa and seed contact with the pathogen at 12 hpi is a method that can be used for inoculation of F. meridionale in wheat seeds. | ||
| 650 | 4 | |a Fusarium head blight |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Germination |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Mannitol |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Seed pathology |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Water potential |7 (dpeaa)DE-He213 | |
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| 700 | 1 | |a Milanesi, Paola Mendes |0 (orcid)0000-0001-7319-916X |4 aut | |
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10.1007/s10658-022-02621-8 doi (DE-627)SPR049319108 (SPR)s10658-022-02621-8-e DE-627 ger DE-627 rakwb eng de Macedo Nava, Francine Falcão verfasserin (orcid)0000-0003-0216-305X aut Water restriction technique for inoculation of Fusarium meridionale in wheat seeds 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Koninklijke Nederlandse Planteziektenkundige Vereniging 2023 Abstract Fusarium head blight (FHB) is a disease of wheat caused by the Fusarium graminearum species complex in which Fusarium meridionale is included. The seed is an inoculum source and the use of inoculated seeds for this pathosystem studies can optimize the time of experimental tests. However early germination of seed during the inoculation process can make studies on the FHB pathosystem very challenging. Therefore, this study aimed to determine the optimal water restriction condition and time of exposure for inoculation of F. meridionale in wheat seeds without reducing seed germination, to define a reliable and rapid technique for inoculating FHB using wheat seeds. Mycelial discs of F. meridionale were placed in the center of Petri dishes containing potato dextrose agar (PDA) media with different quantities of mannitol to establish water potentials of −0.4, −0.6, −0.8, −1.0 or − 1.2 MPa. A control plate with only PDA had −0.36 MPa. Mycelial growth was measured for 178 hours post inoculation (hpi). Wheat seeds were placed in contact with the mycelium at 12, 24, 36, 48 or 60 hpi. Seed germination and seed pathology were evaluated. The greatest growth of the pathogen was at water potentials between −0.8 and − 1.06 MPa. Seed germination was not affected by the water potential at 12 hpi. It was verified that at −1.0 MPa the seeds did not germinate early, and the pathogen was detected in 100% of the inoculated seeds. Thus, the use of mannitol at water potential of −1.0 MPa and seed contact with the pathogen at 12 hpi is a method that can be used for inoculation of F. meridionale in wheat seeds. Fusarium head blight (dpeaa)DE-He213 Germination (dpeaa)DE-He213 Mannitol (dpeaa)DE-He213 Seed pathology (dpeaa)DE-He213 Water potential (dpeaa)DE-He213 Gallina, Alessandra (orcid)0000-0001-9697-7286 aut Stefanski, Francine Spitza (orcid)0000-0002-5609-7329 aut Tortelli, Brenda (orcid)0000-0002-2062-0114 aut Kehl, Kassiana (orcid)0000-0002-2493-2131 aut Huzar-Novakowiski, Jaqueline (orcid)0000-0003-1037-2396 aut Deuner, Carolina Cardoso (orcid)0000-0001-6010-1610 aut Tonial, Fabiana (orcid)0000-0001-7884-3591 aut Milanesi, Paola Mendes (orcid)0000-0001-7319-916X aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 165(2023), 3 vom: 03. Jan., Seite 603-608 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:165 year:2023 number:3 day:03 month:01 pages:603-608 https://dx.doi.org/10.1007/s10658-022-02621-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 165 2023 3 03 01 603-608 |
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10.1007/s10658-022-02621-8 doi (DE-627)SPR049319108 (SPR)s10658-022-02621-8-e DE-627 ger DE-627 rakwb eng de Macedo Nava, Francine Falcão verfasserin (orcid)0000-0003-0216-305X aut Water restriction technique for inoculation of Fusarium meridionale in wheat seeds 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Koninklijke Nederlandse Planteziektenkundige Vereniging 2023 Abstract Fusarium head blight (FHB) is a disease of wheat caused by the Fusarium graminearum species complex in which Fusarium meridionale is included. The seed is an inoculum source and the use of inoculated seeds for this pathosystem studies can optimize the time of experimental tests. However early germination of seed during the inoculation process can make studies on the FHB pathosystem very challenging. Therefore, this study aimed to determine the optimal water restriction condition and time of exposure for inoculation of F. meridionale in wheat seeds without reducing seed germination, to define a reliable and rapid technique for inoculating FHB using wheat seeds. Mycelial discs of F. meridionale were placed in the center of Petri dishes containing potato dextrose agar (PDA) media with different quantities of mannitol to establish water potentials of −0.4, −0.6, −0.8, −1.0 or − 1.2 MPa. A control plate with only PDA had −0.36 MPa. Mycelial growth was measured for 178 hours post inoculation (hpi). Wheat seeds were placed in contact with the mycelium at 12, 24, 36, 48 or 60 hpi. Seed germination and seed pathology were evaluated. The greatest growth of the pathogen was at water potentials between −0.8 and − 1.06 MPa. Seed germination was not affected by the water potential at 12 hpi. It was verified that at −1.0 MPa the seeds did not germinate early, and the pathogen was detected in 100% of the inoculated seeds. Thus, the use of mannitol at water potential of −1.0 MPa and seed contact with the pathogen at 12 hpi is a method that can be used for inoculation of F. meridionale in wheat seeds. Fusarium head blight (dpeaa)DE-He213 Germination (dpeaa)DE-He213 Mannitol (dpeaa)DE-He213 Seed pathology (dpeaa)DE-He213 Water potential (dpeaa)DE-He213 Gallina, Alessandra (orcid)0000-0001-9697-7286 aut Stefanski, Francine Spitza (orcid)0000-0002-5609-7329 aut Tortelli, Brenda (orcid)0000-0002-2062-0114 aut Kehl, Kassiana (orcid)0000-0002-2493-2131 aut Huzar-Novakowiski, Jaqueline (orcid)0000-0003-1037-2396 aut Deuner, Carolina Cardoso (orcid)0000-0001-6010-1610 aut Tonial, Fabiana (orcid)0000-0001-7884-3591 aut Milanesi, Paola Mendes (orcid)0000-0001-7319-916X aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 165(2023), 3 vom: 03. Jan., Seite 603-608 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:165 year:2023 number:3 day:03 month:01 pages:603-608 https://dx.doi.org/10.1007/s10658-022-02621-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 165 2023 3 03 01 603-608 |
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10.1007/s10658-022-02621-8 doi (DE-627)SPR049319108 (SPR)s10658-022-02621-8-e DE-627 ger DE-627 rakwb eng de Macedo Nava, Francine Falcão verfasserin (orcid)0000-0003-0216-305X aut Water restriction technique for inoculation of Fusarium meridionale in wheat seeds 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Koninklijke Nederlandse Planteziektenkundige Vereniging 2023 Abstract Fusarium head blight (FHB) is a disease of wheat caused by the Fusarium graminearum species complex in which Fusarium meridionale is included. The seed is an inoculum source and the use of inoculated seeds for this pathosystem studies can optimize the time of experimental tests. However early germination of seed during the inoculation process can make studies on the FHB pathosystem very challenging. Therefore, this study aimed to determine the optimal water restriction condition and time of exposure for inoculation of F. meridionale in wheat seeds without reducing seed germination, to define a reliable and rapid technique for inoculating FHB using wheat seeds. Mycelial discs of F. meridionale were placed in the center of Petri dishes containing potato dextrose agar (PDA) media with different quantities of mannitol to establish water potentials of −0.4, −0.6, −0.8, −1.0 or − 1.2 MPa. A control plate with only PDA had −0.36 MPa. Mycelial growth was measured for 178 hours post inoculation (hpi). Wheat seeds were placed in contact with the mycelium at 12, 24, 36, 48 or 60 hpi. Seed germination and seed pathology were evaluated. The greatest growth of the pathogen was at water potentials between −0.8 and − 1.06 MPa. Seed germination was not affected by the water potential at 12 hpi. It was verified that at −1.0 MPa the seeds did not germinate early, and the pathogen was detected in 100% of the inoculated seeds. Thus, the use of mannitol at water potential of −1.0 MPa and seed contact with the pathogen at 12 hpi is a method that can be used for inoculation of F. meridionale in wheat seeds. Fusarium head blight (dpeaa)DE-He213 Germination (dpeaa)DE-He213 Mannitol (dpeaa)DE-He213 Seed pathology (dpeaa)DE-He213 Water potential (dpeaa)DE-He213 Gallina, Alessandra (orcid)0000-0001-9697-7286 aut Stefanski, Francine Spitza (orcid)0000-0002-5609-7329 aut Tortelli, Brenda (orcid)0000-0002-2062-0114 aut Kehl, Kassiana (orcid)0000-0002-2493-2131 aut Huzar-Novakowiski, Jaqueline (orcid)0000-0003-1037-2396 aut Deuner, Carolina Cardoso (orcid)0000-0001-6010-1610 aut Tonial, Fabiana (orcid)0000-0001-7884-3591 aut Milanesi, Paola Mendes (orcid)0000-0001-7319-916X aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 165(2023), 3 vom: 03. Jan., Seite 603-608 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:165 year:2023 number:3 day:03 month:01 pages:603-608 https://dx.doi.org/10.1007/s10658-022-02621-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 165 2023 3 03 01 603-608 |
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10.1007/s10658-022-02621-8 doi (DE-627)SPR049319108 (SPR)s10658-022-02621-8-e DE-627 ger DE-627 rakwb eng de Macedo Nava, Francine Falcão verfasserin (orcid)0000-0003-0216-305X aut Water restriction technique for inoculation of Fusarium meridionale in wheat seeds 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Koninklijke Nederlandse Planteziektenkundige Vereniging 2023 Abstract Fusarium head blight (FHB) is a disease of wheat caused by the Fusarium graminearum species complex in which Fusarium meridionale is included. The seed is an inoculum source and the use of inoculated seeds for this pathosystem studies can optimize the time of experimental tests. However early germination of seed during the inoculation process can make studies on the FHB pathosystem very challenging. Therefore, this study aimed to determine the optimal water restriction condition and time of exposure for inoculation of F. meridionale in wheat seeds without reducing seed germination, to define a reliable and rapid technique for inoculating FHB using wheat seeds. Mycelial discs of F. meridionale were placed in the center of Petri dishes containing potato dextrose agar (PDA) media with different quantities of mannitol to establish water potentials of −0.4, −0.6, −0.8, −1.0 or − 1.2 MPa. A control plate with only PDA had −0.36 MPa. Mycelial growth was measured for 178 hours post inoculation (hpi). Wheat seeds were placed in contact with the mycelium at 12, 24, 36, 48 or 60 hpi. Seed germination and seed pathology were evaluated. The greatest growth of the pathogen was at water potentials between −0.8 and − 1.06 MPa. Seed germination was not affected by the water potential at 12 hpi. It was verified that at −1.0 MPa the seeds did not germinate early, and the pathogen was detected in 100% of the inoculated seeds. Thus, the use of mannitol at water potential of −1.0 MPa and seed contact with the pathogen at 12 hpi is a method that can be used for inoculation of F. meridionale in wheat seeds. Fusarium head blight (dpeaa)DE-He213 Germination (dpeaa)DE-He213 Mannitol (dpeaa)DE-He213 Seed pathology (dpeaa)DE-He213 Water potential (dpeaa)DE-He213 Gallina, Alessandra (orcid)0000-0001-9697-7286 aut Stefanski, Francine Spitza (orcid)0000-0002-5609-7329 aut Tortelli, Brenda (orcid)0000-0002-2062-0114 aut Kehl, Kassiana (orcid)0000-0002-2493-2131 aut Huzar-Novakowiski, Jaqueline (orcid)0000-0003-1037-2396 aut Deuner, Carolina Cardoso (orcid)0000-0001-6010-1610 aut Tonial, Fabiana (orcid)0000-0001-7884-3591 aut Milanesi, Paola Mendes (orcid)0000-0001-7319-916X aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 165(2023), 3 vom: 03. Jan., Seite 603-608 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:165 year:2023 number:3 day:03 month:01 pages:603-608 https://dx.doi.org/10.1007/s10658-022-02621-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 165 2023 3 03 01 603-608 |
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10.1007/s10658-022-02621-8 doi (DE-627)SPR049319108 (SPR)s10658-022-02621-8-e DE-627 ger DE-627 rakwb eng de Macedo Nava, Francine Falcão verfasserin (orcid)0000-0003-0216-305X aut Water restriction technique for inoculation of Fusarium meridionale in wheat seeds 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Koninklijke Nederlandse Planteziektenkundige Vereniging 2023 Abstract Fusarium head blight (FHB) is a disease of wheat caused by the Fusarium graminearum species complex in which Fusarium meridionale is included. The seed is an inoculum source and the use of inoculated seeds for this pathosystem studies can optimize the time of experimental tests. However early germination of seed during the inoculation process can make studies on the FHB pathosystem very challenging. Therefore, this study aimed to determine the optimal water restriction condition and time of exposure for inoculation of F. meridionale in wheat seeds without reducing seed germination, to define a reliable and rapid technique for inoculating FHB using wheat seeds. Mycelial discs of F. meridionale were placed in the center of Petri dishes containing potato dextrose agar (PDA) media with different quantities of mannitol to establish water potentials of −0.4, −0.6, −0.8, −1.0 or − 1.2 MPa. A control plate with only PDA had −0.36 MPa. Mycelial growth was measured for 178 hours post inoculation (hpi). Wheat seeds were placed in contact with the mycelium at 12, 24, 36, 48 or 60 hpi. Seed germination and seed pathology were evaluated. The greatest growth of the pathogen was at water potentials between −0.8 and − 1.06 MPa. Seed germination was not affected by the water potential at 12 hpi. It was verified that at −1.0 MPa the seeds did not germinate early, and the pathogen was detected in 100% of the inoculated seeds. Thus, the use of mannitol at water potential of −1.0 MPa and seed contact with the pathogen at 12 hpi is a method that can be used for inoculation of F. meridionale in wheat seeds. Fusarium head blight (dpeaa)DE-He213 Germination (dpeaa)DE-He213 Mannitol (dpeaa)DE-He213 Seed pathology (dpeaa)DE-He213 Water potential (dpeaa)DE-He213 Gallina, Alessandra (orcid)0000-0001-9697-7286 aut Stefanski, Francine Spitza (orcid)0000-0002-5609-7329 aut Tortelli, Brenda (orcid)0000-0002-2062-0114 aut Kehl, Kassiana (orcid)0000-0002-2493-2131 aut Huzar-Novakowiski, Jaqueline (orcid)0000-0003-1037-2396 aut Deuner, Carolina Cardoso (orcid)0000-0001-6010-1610 aut Tonial, Fabiana (orcid)0000-0001-7884-3591 aut Milanesi, Paola Mendes (orcid)0000-0001-7319-916X aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 165(2023), 3 vom: 03. Jan., Seite 603-608 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:165 year:2023 number:3 day:03 month:01 pages:603-608 https://dx.doi.org/10.1007/s10658-022-02621-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 165 2023 3 03 01 603-608 |
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Enthalten in European journal of plant pathology 165(2023), 3 vom: 03. Jan., Seite 603-608 volume:165 year:2023 number:3 day:03 month:01 pages:603-608 |
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de Macedo Nava, Francine Falcão @@aut@@ Gallina, Alessandra @@aut@@ Stefanski, Francine Spitza @@aut@@ Tortelli, Brenda @@aut@@ Kehl, Kassiana @@aut@@ Huzar-Novakowiski, Jaqueline @@aut@@ Deuner, Carolina Cardoso @@aut@@ Tonial, Fabiana @@aut@@ Milanesi, Paola Mendes @@aut@@ |
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The seed is an inoculum source and the use of inoculated seeds for this pathosystem studies can optimize the time of experimental tests. However early germination of seed during the inoculation process can make studies on the FHB pathosystem very challenging. Therefore, this study aimed to determine the optimal water restriction condition and time of exposure for inoculation of F. meridionale in wheat seeds without reducing seed germination, to define a reliable and rapid technique for inoculating FHB using wheat seeds. Mycelial discs of F. meridionale were placed in the center of Petri dishes containing potato dextrose agar (PDA) media with different quantities of mannitol to establish water potentials of −0.4, −0.6, −0.8, −1.0 or − 1.2 MPa. A control plate with only PDA had −0.36 MPa. Mycelial growth was measured for 178 hours post inoculation (hpi). Wheat seeds were placed in contact with the mycelium at 12, 24, 36, 48 or 60 hpi. Seed germination and seed pathology were evaluated. The greatest growth of the pathogen was at water potentials between −0.8 and − 1.06 MPa. Seed germination was not affected by the water potential at 12 hpi. It was verified that at −1.0 MPa the seeds did not germinate early, and the pathogen was detected in 100% of the inoculated seeds. 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de Macedo Nava, Francine Falcão |
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de Macedo Nava, Francine Falcão misc Fusarium head blight misc Germination misc Mannitol misc Seed pathology misc Water potential Water restriction technique for inoculation of Fusarium meridionale in wheat seeds |
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Water restriction technique for inoculation of Fusarium meridionale in wheat seeds Fusarium head blight (dpeaa)DE-He213 Germination (dpeaa)DE-He213 Mannitol (dpeaa)DE-He213 Seed pathology (dpeaa)DE-He213 Water potential (dpeaa)DE-He213 |
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Water restriction technique for inoculation of Fusarium meridionale in wheat seeds |
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de Macedo Nava, Francine Falcão |
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European journal of plant pathology |
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de Macedo Nava, Francine Falcão Gallina, Alessandra Stefanski, Francine Spitza Tortelli, Brenda Kehl, Kassiana Huzar-Novakowiski, Jaqueline Deuner, Carolina Cardoso Tonial, Fabiana Milanesi, Paola Mendes |
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de Macedo Nava, Francine Falcão |
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| title_sort |
water restriction technique for inoculation of fusarium meridionale in wheat seeds |
| title_auth |
Water restriction technique for inoculation of Fusarium meridionale in wheat seeds |
| abstract |
Abstract Fusarium head blight (FHB) is a disease of wheat caused by the Fusarium graminearum species complex in which Fusarium meridionale is included. The seed is an inoculum source and the use of inoculated seeds for this pathosystem studies can optimize the time of experimental tests. However early germination of seed during the inoculation process can make studies on the FHB pathosystem very challenging. Therefore, this study aimed to determine the optimal water restriction condition and time of exposure for inoculation of F. meridionale in wheat seeds without reducing seed germination, to define a reliable and rapid technique for inoculating FHB using wheat seeds. Mycelial discs of F. meridionale were placed in the center of Petri dishes containing potato dextrose agar (PDA) media with different quantities of mannitol to establish water potentials of −0.4, −0.6, −0.8, −1.0 or − 1.2 MPa. A control plate with only PDA had −0.36 MPa. Mycelial growth was measured for 178 hours post inoculation (hpi). Wheat seeds were placed in contact with the mycelium at 12, 24, 36, 48 or 60 hpi. Seed germination and seed pathology were evaluated. The greatest growth of the pathogen was at water potentials between −0.8 and − 1.06 MPa. Seed germination was not affected by the water potential at 12 hpi. It was verified that at −1.0 MPa the seeds did not germinate early, and the pathogen was detected in 100% of the inoculated seeds. Thus, the use of mannitol at water potential of −1.0 MPa and seed contact with the pathogen at 12 hpi is a method that can be used for inoculation of F. meridionale in wheat seeds. © Koninklijke Nederlandse Planteziektenkundige Vereniging 2023 |
| abstractGer |
Abstract Fusarium head blight (FHB) is a disease of wheat caused by the Fusarium graminearum species complex in which Fusarium meridionale is included. The seed is an inoculum source and the use of inoculated seeds for this pathosystem studies can optimize the time of experimental tests. However early germination of seed during the inoculation process can make studies on the FHB pathosystem very challenging. Therefore, this study aimed to determine the optimal water restriction condition and time of exposure for inoculation of F. meridionale in wheat seeds without reducing seed germination, to define a reliable and rapid technique for inoculating FHB using wheat seeds. Mycelial discs of F. meridionale were placed in the center of Petri dishes containing potato dextrose agar (PDA) media with different quantities of mannitol to establish water potentials of −0.4, −0.6, −0.8, −1.0 or − 1.2 MPa. A control plate with only PDA had −0.36 MPa. Mycelial growth was measured for 178 hours post inoculation (hpi). Wheat seeds were placed in contact with the mycelium at 12, 24, 36, 48 or 60 hpi. Seed germination and seed pathology were evaluated. The greatest growth of the pathogen was at water potentials between −0.8 and − 1.06 MPa. Seed germination was not affected by the water potential at 12 hpi. It was verified that at −1.0 MPa the seeds did not germinate early, and the pathogen was detected in 100% of the inoculated seeds. Thus, the use of mannitol at water potential of −1.0 MPa and seed contact with the pathogen at 12 hpi is a method that can be used for inoculation of F. meridionale in wheat seeds. © Koninklijke Nederlandse Planteziektenkundige Vereniging 2023 |
| abstract_unstemmed |
Abstract Fusarium head blight (FHB) is a disease of wheat caused by the Fusarium graminearum species complex in which Fusarium meridionale is included. The seed is an inoculum source and the use of inoculated seeds for this pathosystem studies can optimize the time of experimental tests. However early germination of seed during the inoculation process can make studies on the FHB pathosystem very challenging. Therefore, this study aimed to determine the optimal water restriction condition and time of exposure for inoculation of F. meridionale in wheat seeds without reducing seed germination, to define a reliable and rapid technique for inoculating FHB using wheat seeds. Mycelial discs of F. meridionale were placed in the center of Petri dishes containing potato dextrose agar (PDA) media with different quantities of mannitol to establish water potentials of −0.4, −0.6, −0.8, −1.0 or − 1.2 MPa. A control plate with only PDA had −0.36 MPa. Mycelial growth was measured for 178 hours post inoculation (hpi). Wheat seeds were placed in contact with the mycelium at 12, 24, 36, 48 or 60 hpi. Seed germination and seed pathology were evaluated. The greatest growth of the pathogen was at water potentials between −0.8 and − 1.06 MPa. Seed germination was not affected by the water potential at 12 hpi. It was verified that at −1.0 MPa the seeds did not germinate early, and the pathogen was detected in 100% of the inoculated seeds. Thus, the use of mannitol at water potential of −1.0 MPa and seed contact with the pathogen at 12 hpi is a method that can be used for inoculation of F. meridionale in wheat seeds. © Koninklijke Nederlandse Planteziektenkundige Vereniging 2023 |
| collection_details |
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| container_issue |
3 |
| title_short |
Water restriction technique for inoculation of Fusarium meridionale in wheat seeds |
| url |
https://dx.doi.org/10.1007/s10658-022-02621-8 |
| remote_bool |
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| author2 |
Gallina, Alessandra Stefanski, Francine Spitza Tortelli, Brenda Kehl, Kassiana Huzar-Novakowiski, Jaqueline Deuner, Carolina Cardoso Tonial, Fabiana Milanesi, Paola Mendes |
| author2Str |
Gallina, Alessandra Stefanski, Francine Spitza Tortelli, Brenda Kehl, Kassiana Huzar-Novakowiski, Jaqueline Deuner, Carolina Cardoso Tonial, Fabiana Milanesi, Paola Mendes |
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| doi_str |
10.1007/s10658-022-02621-8 |
| up_date |
2024-07-04T00:18:58.887Z |
| _version_ |
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| score |
7.4019136 |