Isolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalis
Background Antimicrobial resistance is a growing global health concern demanding more attention and action at the international-, national- and regional levels. In the present study, bacteriophage was sought as a potential alternative to traditional antibiotics. Results Vancomycin-resistant Enteroco...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Ali, Zienab [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2023 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s) 2023 |
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| Übergeordnetes Werk: |
Enthalten in: Beni-Suef University Journal of Basic and Applied Sciences - Bani Sweif, 2013, 12(2023), 1 vom: 19. Feb. |
|---|---|
| Übergeordnetes Werk: |
volume:12 ; year:2023 ; number:1 ; day:19 ; month:02 |
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|---|
| DOI / URN: |
10.1186/s43088-023-00362-z |
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SPR049378929 |
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| 520 | |a Background Antimicrobial resistance is a growing global health concern demanding more attention and action at the international-, national- and regional levels. In the present study, bacteriophage was sought as a potential alternative to traditional antibiotics. Results Vancomycin-resistant Enterococcus faecalis was isolated from a urine sample. Partial 16S rRNA-gene sequencing and VITEK®2 system were employed for its identification, biochemical characterization, and antibiotic susceptibility testing. The isolate was resistant to eight antibiotics (out of 11): vancomycin, gentamicin (high-level synergy), streptomycin (high-level synergy), ciprofloxacin, levofloxacin, erythromycin, quinupristin/dalfopristin, and tetracycline. Bacteriophage SA14 was isolated from sewage water using the multidrug-resistant isolate as a host. Transmission electron micrographs revealed that phage SA14 is a member of the Siphoviridae family displaying the typical circular head and long non-contractile tail. The phage showed characteristic stability to a wide range of solution pH and temperatures, with optimal stability at pH 7.4 and 4 °C, while showing high specificity toward their host. Based on the one-step growth curve, the phage's latent period was 25 min, and the burst size was 20 PFU/ml. The lytic activity of phage SA14 was evaluated at various multiplicities of infection (MOI), all considerably suppressed the growth of the host organism. Moreover, phage SA14 displayed a characteristic anti-biofilm activity as observed by the reduction in adhered biomass and -viable cells in the pre-formed biofilm by 19.1-fold and 2.5-fold, respectively. Conclusion Phage therapy can be a valuable alternative to antibiotics against multi-drug resistant microorganisms. | ||
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10.1186/s43088-023-00362-z doi (DE-627)SPR049378929 (SPR)s43088-023-00362-z-e DE-627 ger DE-627 rakwb eng Ali, Zienab verfasserin (orcid)0000-0003-0188-7654 aut Isolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalis 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background Antimicrobial resistance is a growing global health concern demanding more attention and action at the international-, national- and regional levels. In the present study, bacteriophage was sought as a potential alternative to traditional antibiotics. Results Vancomycin-resistant Enterococcus faecalis was isolated from a urine sample. Partial 16S rRNA-gene sequencing and VITEK®2 system were employed for its identification, biochemical characterization, and antibiotic susceptibility testing. The isolate was resistant to eight antibiotics (out of 11): vancomycin, gentamicin (high-level synergy), streptomycin (high-level synergy), ciprofloxacin, levofloxacin, erythromycin, quinupristin/dalfopristin, and tetracycline. Bacteriophage SA14 was isolated from sewage water using the multidrug-resistant isolate as a host. Transmission electron micrographs revealed that phage SA14 is a member of the Siphoviridae family displaying the typical circular head and long non-contractile tail. The phage showed characteristic stability to a wide range of solution pH and temperatures, with optimal stability at pH 7.4 and 4 °C, while showing high specificity toward their host. Based on the one-step growth curve, the phage's latent period was 25 min, and the burst size was 20 PFU/ml. The lytic activity of phage SA14 was evaluated at various multiplicities of infection (MOI), all considerably suppressed the growth of the host organism. Moreover, phage SA14 displayed a characteristic anti-biofilm activity as observed by the reduction in adhered biomass and -viable cells in the pre-formed biofilm by 19.1-fold and 2.5-fold, respectively. Conclusion Phage therapy can be a valuable alternative to antibiotics against multi-drug resistant microorganisms. Bacteriophage (dpeaa)DE-He213 Resistance (dpeaa)DE-He213 Biofilm (dpeaa)DE-He213 Dishisha, Tarek (orcid)0000-0001-8644-2640 aut El-Gendy, Ahmed O. (orcid)0000-0002-0980-5185 aut Azmy, Ahmed F. (orcid)0000-0002-7347-9564 aut Enthalten in Beni-Suef University Journal of Basic and Applied Sciences Bani Sweif, 2013 12(2023), 1 vom: 19. Feb. (DE-627)784988870 (DE-600)2768279-1 2314-8543 nnns volume:12 year:2023 number:1 day:19 month:02 https://dx.doi.org/10.1186/s43088-023-00362-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_2027 AR 12 2023 1 19 02 |
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10.1186/s43088-023-00362-z doi (DE-627)SPR049378929 (SPR)s43088-023-00362-z-e DE-627 ger DE-627 rakwb eng Ali, Zienab verfasserin (orcid)0000-0003-0188-7654 aut Isolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalis 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background Antimicrobial resistance is a growing global health concern demanding more attention and action at the international-, national- and regional levels. In the present study, bacteriophage was sought as a potential alternative to traditional antibiotics. Results Vancomycin-resistant Enterococcus faecalis was isolated from a urine sample. Partial 16S rRNA-gene sequencing and VITEK®2 system were employed for its identification, biochemical characterization, and antibiotic susceptibility testing. The isolate was resistant to eight antibiotics (out of 11): vancomycin, gentamicin (high-level synergy), streptomycin (high-level synergy), ciprofloxacin, levofloxacin, erythromycin, quinupristin/dalfopristin, and tetracycline. Bacteriophage SA14 was isolated from sewage water using the multidrug-resistant isolate as a host. Transmission electron micrographs revealed that phage SA14 is a member of the Siphoviridae family displaying the typical circular head and long non-contractile tail. The phage showed characteristic stability to a wide range of solution pH and temperatures, with optimal stability at pH 7.4 and 4 °C, while showing high specificity toward their host. Based on the one-step growth curve, the phage's latent period was 25 min, and the burst size was 20 PFU/ml. The lytic activity of phage SA14 was evaluated at various multiplicities of infection (MOI), all considerably suppressed the growth of the host organism. Moreover, phage SA14 displayed a characteristic anti-biofilm activity as observed by the reduction in adhered biomass and -viable cells in the pre-formed biofilm by 19.1-fold and 2.5-fold, respectively. Conclusion Phage therapy can be a valuable alternative to antibiotics against multi-drug resistant microorganisms. Bacteriophage (dpeaa)DE-He213 Resistance (dpeaa)DE-He213 Biofilm (dpeaa)DE-He213 Dishisha, Tarek (orcid)0000-0001-8644-2640 aut El-Gendy, Ahmed O. (orcid)0000-0002-0980-5185 aut Azmy, Ahmed F. (orcid)0000-0002-7347-9564 aut Enthalten in Beni-Suef University Journal of Basic and Applied Sciences Bani Sweif, 2013 12(2023), 1 vom: 19. Feb. (DE-627)784988870 (DE-600)2768279-1 2314-8543 nnns volume:12 year:2023 number:1 day:19 month:02 https://dx.doi.org/10.1186/s43088-023-00362-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_2027 AR 12 2023 1 19 02 |
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10.1186/s43088-023-00362-z doi (DE-627)SPR049378929 (SPR)s43088-023-00362-z-e DE-627 ger DE-627 rakwb eng Ali, Zienab verfasserin (orcid)0000-0003-0188-7654 aut Isolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalis 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background Antimicrobial resistance is a growing global health concern demanding more attention and action at the international-, national- and regional levels. In the present study, bacteriophage was sought as a potential alternative to traditional antibiotics. Results Vancomycin-resistant Enterococcus faecalis was isolated from a urine sample. Partial 16S rRNA-gene sequencing and VITEK®2 system were employed for its identification, biochemical characterization, and antibiotic susceptibility testing. The isolate was resistant to eight antibiotics (out of 11): vancomycin, gentamicin (high-level synergy), streptomycin (high-level synergy), ciprofloxacin, levofloxacin, erythromycin, quinupristin/dalfopristin, and tetracycline. Bacteriophage SA14 was isolated from sewage water using the multidrug-resistant isolate as a host. Transmission electron micrographs revealed that phage SA14 is a member of the Siphoviridae family displaying the typical circular head and long non-contractile tail. The phage showed characteristic stability to a wide range of solution pH and temperatures, with optimal stability at pH 7.4 and 4 °C, while showing high specificity toward their host. Based on the one-step growth curve, the phage's latent period was 25 min, and the burst size was 20 PFU/ml. The lytic activity of phage SA14 was evaluated at various multiplicities of infection (MOI), all considerably suppressed the growth of the host organism. Moreover, phage SA14 displayed a characteristic anti-biofilm activity as observed by the reduction in adhered biomass and -viable cells in the pre-formed biofilm by 19.1-fold and 2.5-fold, respectively. Conclusion Phage therapy can be a valuable alternative to antibiotics against multi-drug resistant microorganisms. Bacteriophage (dpeaa)DE-He213 Resistance (dpeaa)DE-He213 Biofilm (dpeaa)DE-He213 Dishisha, Tarek (orcid)0000-0001-8644-2640 aut El-Gendy, Ahmed O. (orcid)0000-0002-0980-5185 aut Azmy, Ahmed F. (orcid)0000-0002-7347-9564 aut Enthalten in Beni-Suef University Journal of Basic and Applied Sciences Bani Sweif, 2013 12(2023), 1 vom: 19. Feb. (DE-627)784988870 (DE-600)2768279-1 2314-8543 nnns volume:12 year:2023 number:1 day:19 month:02 https://dx.doi.org/10.1186/s43088-023-00362-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_2027 AR 12 2023 1 19 02 |
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10.1186/s43088-023-00362-z doi (DE-627)SPR049378929 (SPR)s43088-023-00362-z-e DE-627 ger DE-627 rakwb eng Ali, Zienab verfasserin (orcid)0000-0003-0188-7654 aut Isolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalis 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background Antimicrobial resistance is a growing global health concern demanding more attention and action at the international-, national- and regional levels. In the present study, bacteriophage was sought as a potential alternative to traditional antibiotics. Results Vancomycin-resistant Enterococcus faecalis was isolated from a urine sample. Partial 16S rRNA-gene sequencing and VITEK®2 system were employed for its identification, biochemical characterization, and antibiotic susceptibility testing. The isolate was resistant to eight antibiotics (out of 11): vancomycin, gentamicin (high-level synergy), streptomycin (high-level synergy), ciprofloxacin, levofloxacin, erythromycin, quinupristin/dalfopristin, and tetracycline. Bacteriophage SA14 was isolated from sewage water using the multidrug-resistant isolate as a host. Transmission electron micrographs revealed that phage SA14 is a member of the Siphoviridae family displaying the typical circular head and long non-contractile tail. The phage showed characteristic stability to a wide range of solution pH and temperatures, with optimal stability at pH 7.4 and 4 °C, while showing high specificity toward their host. Based on the one-step growth curve, the phage's latent period was 25 min, and the burst size was 20 PFU/ml. The lytic activity of phage SA14 was evaluated at various multiplicities of infection (MOI), all considerably suppressed the growth of the host organism. Moreover, phage SA14 displayed a characteristic anti-biofilm activity as observed by the reduction in adhered biomass and -viable cells in the pre-formed biofilm by 19.1-fold and 2.5-fold, respectively. Conclusion Phage therapy can be a valuable alternative to antibiotics against multi-drug resistant microorganisms. Bacteriophage (dpeaa)DE-He213 Resistance (dpeaa)DE-He213 Biofilm (dpeaa)DE-He213 Dishisha, Tarek (orcid)0000-0001-8644-2640 aut El-Gendy, Ahmed O. (orcid)0000-0002-0980-5185 aut Azmy, Ahmed F. (orcid)0000-0002-7347-9564 aut Enthalten in Beni-Suef University Journal of Basic and Applied Sciences Bani Sweif, 2013 12(2023), 1 vom: 19. Feb. (DE-627)784988870 (DE-600)2768279-1 2314-8543 nnns volume:12 year:2023 number:1 day:19 month:02 https://dx.doi.org/10.1186/s43088-023-00362-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_2027 AR 12 2023 1 19 02 |
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10.1186/s43088-023-00362-z doi (DE-627)SPR049378929 (SPR)s43088-023-00362-z-e DE-627 ger DE-627 rakwb eng Ali, Zienab verfasserin (orcid)0000-0003-0188-7654 aut Isolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalis 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background Antimicrobial resistance is a growing global health concern demanding more attention and action at the international-, national- and regional levels. In the present study, bacteriophage was sought as a potential alternative to traditional antibiotics. Results Vancomycin-resistant Enterococcus faecalis was isolated from a urine sample. Partial 16S rRNA-gene sequencing and VITEK®2 system were employed for its identification, biochemical characterization, and antibiotic susceptibility testing. The isolate was resistant to eight antibiotics (out of 11): vancomycin, gentamicin (high-level synergy), streptomycin (high-level synergy), ciprofloxacin, levofloxacin, erythromycin, quinupristin/dalfopristin, and tetracycline. Bacteriophage SA14 was isolated from sewage water using the multidrug-resistant isolate as a host. Transmission electron micrographs revealed that phage SA14 is a member of the Siphoviridae family displaying the typical circular head and long non-contractile tail. The phage showed characteristic stability to a wide range of solution pH and temperatures, with optimal stability at pH 7.4 and 4 °C, while showing high specificity toward their host. Based on the one-step growth curve, the phage's latent period was 25 min, and the burst size was 20 PFU/ml. The lytic activity of phage SA14 was evaluated at various multiplicities of infection (MOI), all considerably suppressed the growth of the host organism. Moreover, phage SA14 displayed a characteristic anti-biofilm activity as observed by the reduction in adhered biomass and -viable cells in the pre-formed biofilm by 19.1-fold and 2.5-fold, respectively. Conclusion Phage therapy can be a valuable alternative to antibiotics against multi-drug resistant microorganisms. Bacteriophage (dpeaa)DE-He213 Resistance (dpeaa)DE-He213 Biofilm (dpeaa)DE-He213 Dishisha, Tarek (orcid)0000-0001-8644-2640 aut El-Gendy, Ahmed O. (orcid)0000-0002-0980-5185 aut Azmy, Ahmed F. (orcid)0000-0002-7347-9564 aut Enthalten in Beni-Suef University Journal of Basic and Applied Sciences Bani Sweif, 2013 12(2023), 1 vom: 19. Feb. (DE-627)784988870 (DE-600)2768279-1 2314-8543 nnns volume:12 year:2023 number:1 day:19 month:02 https://dx.doi.org/10.1186/s43088-023-00362-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_2027 AR 12 2023 1 19 02 |
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Isolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalis Bacteriophage (dpeaa)DE-He213 Resistance (dpeaa)DE-He213 Biofilm (dpeaa)DE-He213 |
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Isolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalis |
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Isolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalis |
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Ali, Zienab |
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Ali, Zienab Dishisha, Tarek El-Gendy, Ahmed O. Azmy, Ahmed F. |
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Ali, Zienab |
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isolation and phenotypic characterization of bacteriophage sa14 with lytic- and anti-biofilm activity against multidrug-resistant enterococcus faecalis |
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Isolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalis |
| abstract |
Background Antimicrobial resistance is a growing global health concern demanding more attention and action at the international-, national- and regional levels. In the present study, bacteriophage was sought as a potential alternative to traditional antibiotics. Results Vancomycin-resistant Enterococcus faecalis was isolated from a urine sample. Partial 16S rRNA-gene sequencing and VITEK®2 system were employed for its identification, biochemical characterization, and antibiotic susceptibility testing. The isolate was resistant to eight antibiotics (out of 11): vancomycin, gentamicin (high-level synergy), streptomycin (high-level synergy), ciprofloxacin, levofloxacin, erythromycin, quinupristin/dalfopristin, and tetracycline. Bacteriophage SA14 was isolated from sewage water using the multidrug-resistant isolate as a host. Transmission electron micrographs revealed that phage SA14 is a member of the Siphoviridae family displaying the typical circular head and long non-contractile tail. The phage showed characteristic stability to a wide range of solution pH and temperatures, with optimal stability at pH 7.4 and 4 °C, while showing high specificity toward their host. Based on the one-step growth curve, the phage's latent period was 25 min, and the burst size was 20 PFU/ml. The lytic activity of phage SA14 was evaluated at various multiplicities of infection (MOI), all considerably suppressed the growth of the host organism. Moreover, phage SA14 displayed a characteristic anti-biofilm activity as observed by the reduction in adhered biomass and -viable cells in the pre-formed biofilm by 19.1-fold and 2.5-fold, respectively. Conclusion Phage therapy can be a valuable alternative to antibiotics against multi-drug resistant microorganisms. © The Author(s) 2023 |
| abstractGer |
Background Antimicrobial resistance is a growing global health concern demanding more attention and action at the international-, national- and regional levels. In the present study, bacteriophage was sought as a potential alternative to traditional antibiotics. Results Vancomycin-resistant Enterococcus faecalis was isolated from a urine sample. Partial 16S rRNA-gene sequencing and VITEK®2 system were employed for its identification, biochemical characterization, and antibiotic susceptibility testing. The isolate was resistant to eight antibiotics (out of 11): vancomycin, gentamicin (high-level synergy), streptomycin (high-level synergy), ciprofloxacin, levofloxacin, erythromycin, quinupristin/dalfopristin, and tetracycline. Bacteriophage SA14 was isolated from sewage water using the multidrug-resistant isolate as a host. Transmission electron micrographs revealed that phage SA14 is a member of the Siphoviridae family displaying the typical circular head and long non-contractile tail. The phage showed characteristic stability to a wide range of solution pH and temperatures, with optimal stability at pH 7.4 and 4 °C, while showing high specificity toward their host. Based on the one-step growth curve, the phage's latent period was 25 min, and the burst size was 20 PFU/ml. The lytic activity of phage SA14 was evaluated at various multiplicities of infection (MOI), all considerably suppressed the growth of the host organism. Moreover, phage SA14 displayed a characteristic anti-biofilm activity as observed by the reduction in adhered biomass and -viable cells in the pre-formed biofilm by 19.1-fold and 2.5-fold, respectively. Conclusion Phage therapy can be a valuable alternative to antibiotics against multi-drug resistant microorganisms. © The Author(s) 2023 |
| abstract_unstemmed |
Background Antimicrobial resistance is a growing global health concern demanding more attention and action at the international-, national- and regional levels. In the present study, bacteriophage was sought as a potential alternative to traditional antibiotics. Results Vancomycin-resistant Enterococcus faecalis was isolated from a urine sample. Partial 16S rRNA-gene sequencing and VITEK®2 system were employed for its identification, biochemical characterization, and antibiotic susceptibility testing. The isolate was resistant to eight antibiotics (out of 11): vancomycin, gentamicin (high-level synergy), streptomycin (high-level synergy), ciprofloxacin, levofloxacin, erythromycin, quinupristin/dalfopristin, and tetracycline. Bacteriophage SA14 was isolated from sewage water using the multidrug-resistant isolate as a host. Transmission electron micrographs revealed that phage SA14 is a member of the Siphoviridae family displaying the typical circular head and long non-contractile tail. The phage showed characteristic stability to a wide range of solution pH and temperatures, with optimal stability at pH 7.4 and 4 °C, while showing high specificity toward their host. Based on the one-step growth curve, the phage's latent period was 25 min, and the burst size was 20 PFU/ml. The lytic activity of phage SA14 was evaluated at various multiplicities of infection (MOI), all considerably suppressed the growth of the host organism. Moreover, phage SA14 displayed a characteristic anti-biofilm activity as observed by the reduction in adhered biomass and -viable cells in the pre-formed biofilm by 19.1-fold and 2.5-fold, respectively. Conclusion Phage therapy can be a valuable alternative to antibiotics against multi-drug resistant microorganisms. © The Author(s) 2023 |
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Isolation and phenotypic characterization of bacteriophage SA14 with lytic- and anti-biofilm activity against multidrug-resistant Enterococcus faecalis |
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