Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of $ MnO_{2} $ nanosheets
Hepatitis E virus (HEV) is an evolving infectious entity that causes viral hepatitis infections worldwide. Current routine methods of identifying and diagnosing HEV are someway laborious and costly. Based on the biomimicking oxidase-like activity of $ MnO_{2} $ nanosheets, we designed a label-free,...
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| Autor*in: |
Alam, Naveed [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag GmbH Germany, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| Übergeordnetes Werk: |
Enthalten in: Analytical and bioanalytical chemistry - Berlin : Springer, 2002, 415(2022), 4 vom: 05. Dez., Seite 703-713 |
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| Übergeordnetes Werk: |
volume:415 ; year:2022 ; number:4 ; day:05 ; month:12 ; pages:703-713 |
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| DOI / URN: |
10.1007/s00216-022-04461-1 |
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| Katalog-ID: |
SPR049419994 |
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| 245 | 1 | 0 | |a Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of $ MnO_{2} $ nanosheets |
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| 520 | |a Hepatitis E virus (HEV) is an evolving infectious entity that causes viral hepatitis infections worldwide. Current routine methods of identifying and diagnosing HEV are someway laborious and costly. Based on the biomimicking oxidase-like activity of $ MnO_{2} $ nanosheets, we designed a label-free, highly sensitive colorimetric sensing technique for HEV detection. The prepared $ MnO_{2} $ catalyst displays intrinsic biomimicking oxidase-like catalytic activity and efficiently oxidizes the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate from colorless to blue colored oxidized TMB (oxTMB) product which can be measured at 652 nm by UV–visible spectrum. When the HEV-DNA was added, DNA adsorbed easily on $ MnO_{2} $ surface through physical adsorption and electrostatic interaction which hinders the oxidase-like catalytic activity of $ MnO_{2} $. Upon the introduction of target, the HEV target DNA binds with its complementary ssDNA on the surface of $ MnO_{2} $, the hybridized DNA releases from the surface of $ MnO_{2} $, which leads to recovery of oxidase-like catalytic activity of $ MnO_{2} $. This strategy was applied to construct a colorimetric technique for HEV detection. The approach works in the linear range of 1 fM–100 nM DNA concentration with the limit of detection (LOD) of 3.26 fM (S/N = 3) and quantitative limit (LOQ) of 36.08 fM. The TMB-$ MnO_{2} $ platform was highly selective for HEV target DNA detection when compared with potential interferences. Result of serum sample analysis demonstrates that this sensing system can be used for clinical diagnostic applications. Graphical Abstract | ||
| 650 | 4 | |a Hepatitis E virus |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a MnO |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Nanozymes |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a DNA |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Oxidase-mimicking |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Biosensors |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Ravikumar, Chandan Hunsur |4 aut | |
| 700 | 1 | |a Sreeramareddygari, Muralikrishna |4 aut | |
| 700 | 1 | |a Somasundrum, Mithran |4 aut | |
| 700 | 1 | |a Surareungchai, Werasak |4 aut | |
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10.1007/s00216-022-04461-1 doi (DE-627)SPR049419994 (SPR)s00216-022-04461-1-e DE-627 ger DE-627 rakwb eng Alam, Naveed verfasserin aut Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of $ MnO_{2} $ nanosheets 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Hepatitis E virus (HEV) is an evolving infectious entity that causes viral hepatitis infections worldwide. Current routine methods of identifying and diagnosing HEV are someway laborious and costly. Based on the biomimicking oxidase-like activity of $ MnO_{2} $ nanosheets, we designed a label-free, highly sensitive colorimetric sensing technique for HEV detection. The prepared $ MnO_{2} $ catalyst displays intrinsic biomimicking oxidase-like catalytic activity and efficiently oxidizes the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate from colorless to blue colored oxidized TMB (oxTMB) product which can be measured at 652 nm by UV–visible spectrum. When the HEV-DNA was added, DNA adsorbed easily on $ MnO_{2} $ surface through physical adsorption and electrostatic interaction which hinders the oxidase-like catalytic activity of $ MnO_{2} $. Upon the introduction of target, the HEV target DNA binds with its complementary ssDNA on the surface of $ MnO_{2} $, the hybridized DNA releases from the surface of $ MnO_{2} $, which leads to recovery of oxidase-like catalytic activity of $ MnO_{2} $. This strategy was applied to construct a colorimetric technique for HEV detection. The approach works in the linear range of 1 fM–100 nM DNA concentration with the limit of detection (LOD) of 3.26 fM (S/N = 3) and quantitative limit (LOQ) of 36.08 fM. The TMB-$ MnO_{2} $ platform was highly selective for HEV target DNA detection when compared with potential interferences. Result of serum sample analysis demonstrates that this sensing system can be used for clinical diagnostic applications. Graphical Abstract Hepatitis E virus (dpeaa)DE-He213 MnO (dpeaa)DE-He213 Nanozymes (dpeaa)DE-He213 DNA (dpeaa)DE-He213 Oxidase-mimicking (dpeaa)DE-He213 Biosensors (dpeaa)DE-He213 Ravikumar, Chandan Hunsur aut Sreeramareddygari, Muralikrishna aut Somasundrum, Mithran aut Surareungchai, Werasak aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 415(2022), 4 vom: 05. Dez., Seite 703-713 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:415 year:2022 number:4 day:05 month:12 pages:703-713 https://dx.doi.org/10.1007/s00216-022-04461-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 415 2022 4 05 12 703-713 |
| spelling |
10.1007/s00216-022-04461-1 doi (DE-627)SPR049419994 (SPR)s00216-022-04461-1-e DE-627 ger DE-627 rakwb eng Alam, Naveed verfasserin aut Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of $ MnO_{2} $ nanosheets 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Hepatitis E virus (HEV) is an evolving infectious entity that causes viral hepatitis infections worldwide. Current routine methods of identifying and diagnosing HEV are someway laborious and costly. Based on the biomimicking oxidase-like activity of $ MnO_{2} $ nanosheets, we designed a label-free, highly sensitive colorimetric sensing technique for HEV detection. The prepared $ MnO_{2} $ catalyst displays intrinsic biomimicking oxidase-like catalytic activity and efficiently oxidizes the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate from colorless to blue colored oxidized TMB (oxTMB) product which can be measured at 652 nm by UV–visible spectrum. When the HEV-DNA was added, DNA adsorbed easily on $ MnO_{2} $ surface through physical adsorption and electrostatic interaction which hinders the oxidase-like catalytic activity of $ MnO_{2} $. Upon the introduction of target, the HEV target DNA binds with its complementary ssDNA on the surface of $ MnO_{2} $, the hybridized DNA releases from the surface of $ MnO_{2} $, which leads to recovery of oxidase-like catalytic activity of $ MnO_{2} $. This strategy was applied to construct a colorimetric technique for HEV detection. The approach works in the linear range of 1 fM–100 nM DNA concentration with the limit of detection (LOD) of 3.26 fM (S/N = 3) and quantitative limit (LOQ) of 36.08 fM. The TMB-$ MnO_{2} $ platform was highly selective for HEV target DNA detection when compared with potential interferences. Result of serum sample analysis demonstrates that this sensing system can be used for clinical diagnostic applications. Graphical Abstract Hepatitis E virus (dpeaa)DE-He213 MnO (dpeaa)DE-He213 Nanozymes (dpeaa)DE-He213 DNA (dpeaa)DE-He213 Oxidase-mimicking (dpeaa)DE-He213 Biosensors (dpeaa)DE-He213 Ravikumar, Chandan Hunsur aut Sreeramareddygari, Muralikrishna aut Somasundrum, Mithran aut Surareungchai, Werasak aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 415(2022), 4 vom: 05. Dez., Seite 703-713 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:415 year:2022 number:4 day:05 month:12 pages:703-713 https://dx.doi.org/10.1007/s00216-022-04461-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 415 2022 4 05 12 703-713 |
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10.1007/s00216-022-04461-1 doi (DE-627)SPR049419994 (SPR)s00216-022-04461-1-e DE-627 ger DE-627 rakwb eng Alam, Naveed verfasserin aut Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of $ MnO_{2} $ nanosheets 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Hepatitis E virus (HEV) is an evolving infectious entity that causes viral hepatitis infections worldwide. Current routine methods of identifying and diagnosing HEV are someway laborious and costly. Based on the biomimicking oxidase-like activity of $ MnO_{2} $ nanosheets, we designed a label-free, highly sensitive colorimetric sensing technique for HEV detection. The prepared $ MnO_{2} $ catalyst displays intrinsic biomimicking oxidase-like catalytic activity and efficiently oxidizes the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate from colorless to blue colored oxidized TMB (oxTMB) product which can be measured at 652 nm by UV–visible spectrum. When the HEV-DNA was added, DNA adsorbed easily on $ MnO_{2} $ surface through physical adsorption and electrostatic interaction which hinders the oxidase-like catalytic activity of $ MnO_{2} $. Upon the introduction of target, the HEV target DNA binds with its complementary ssDNA on the surface of $ MnO_{2} $, the hybridized DNA releases from the surface of $ MnO_{2} $, which leads to recovery of oxidase-like catalytic activity of $ MnO_{2} $. This strategy was applied to construct a colorimetric technique for HEV detection. The approach works in the linear range of 1 fM–100 nM DNA concentration with the limit of detection (LOD) of 3.26 fM (S/N = 3) and quantitative limit (LOQ) of 36.08 fM. The TMB-$ MnO_{2} $ platform was highly selective for HEV target DNA detection when compared with potential interferences. Result of serum sample analysis demonstrates that this sensing system can be used for clinical diagnostic applications. Graphical Abstract Hepatitis E virus (dpeaa)DE-He213 MnO (dpeaa)DE-He213 Nanozymes (dpeaa)DE-He213 DNA (dpeaa)DE-He213 Oxidase-mimicking (dpeaa)DE-He213 Biosensors (dpeaa)DE-He213 Ravikumar, Chandan Hunsur aut Sreeramareddygari, Muralikrishna aut Somasundrum, Mithran aut Surareungchai, Werasak aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 415(2022), 4 vom: 05. Dez., Seite 703-713 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:415 year:2022 number:4 day:05 month:12 pages:703-713 https://dx.doi.org/10.1007/s00216-022-04461-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 415 2022 4 05 12 703-713 |
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10.1007/s00216-022-04461-1 doi (DE-627)SPR049419994 (SPR)s00216-022-04461-1-e DE-627 ger DE-627 rakwb eng Alam, Naveed verfasserin aut Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of $ MnO_{2} $ nanosheets 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Hepatitis E virus (HEV) is an evolving infectious entity that causes viral hepatitis infections worldwide. Current routine methods of identifying and diagnosing HEV are someway laborious and costly. Based on the biomimicking oxidase-like activity of $ MnO_{2} $ nanosheets, we designed a label-free, highly sensitive colorimetric sensing technique for HEV detection. The prepared $ MnO_{2} $ catalyst displays intrinsic biomimicking oxidase-like catalytic activity and efficiently oxidizes the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate from colorless to blue colored oxidized TMB (oxTMB) product which can be measured at 652 nm by UV–visible spectrum. When the HEV-DNA was added, DNA adsorbed easily on $ MnO_{2} $ surface through physical adsorption and electrostatic interaction which hinders the oxidase-like catalytic activity of $ MnO_{2} $. Upon the introduction of target, the HEV target DNA binds with its complementary ssDNA on the surface of $ MnO_{2} $, the hybridized DNA releases from the surface of $ MnO_{2} $, which leads to recovery of oxidase-like catalytic activity of $ MnO_{2} $. This strategy was applied to construct a colorimetric technique for HEV detection. The approach works in the linear range of 1 fM–100 nM DNA concentration with the limit of detection (LOD) of 3.26 fM (S/N = 3) and quantitative limit (LOQ) of 36.08 fM. The TMB-$ MnO_{2} $ platform was highly selective for HEV target DNA detection when compared with potential interferences. Result of serum sample analysis demonstrates that this sensing system can be used for clinical diagnostic applications. Graphical Abstract Hepatitis E virus (dpeaa)DE-He213 MnO (dpeaa)DE-He213 Nanozymes (dpeaa)DE-He213 DNA (dpeaa)DE-He213 Oxidase-mimicking (dpeaa)DE-He213 Biosensors (dpeaa)DE-He213 Ravikumar, Chandan Hunsur aut Sreeramareddygari, Muralikrishna aut Somasundrum, Mithran aut Surareungchai, Werasak aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 415(2022), 4 vom: 05. Dez., Seite 703-713 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:415 year:2022 number:4 day:05 month:12 pages:703-713 https://dx.doi.org/10.1007/s00216-022-04461-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 415 2022 4 05 12 703-713 |
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10.1007/s00216-022-04461-1 doi (DE-627)SPR049419994 (SPR)s00216-022-04461-1-e DE-627 ger DE-627 rakwb eng Alam, Naveed verfasserin aut Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of $ MnO_{2} $ nanosheets 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Springer-Verlag GmbH Germany, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Hepatitis E virus (HEV) is an evolving infectious entity that causes viral hepatitis infections worldwide. Current routine methods of identifying and diagnosing HEV are someway laborious and costly. Based on the biomimicking oxidase-like activity of $ MnO_{2} $ nanosheets, we designed a label-free, highly sensitive colorimetric sensing technique for HEV detection. The prepared $ MnO_{2} $ catalyst displays intrinsic biomimicking oxidase-like catalytic activity and efficiently oxidizes the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate from colorless to blue colored oxidized TMB (oxTMB) product which can be measured at 652 nm by UV–visible spectrum. When the HEV-DNA was added, DNA adsorbed easily on $ MnO_{2} $ surface through physical adsorption and electrostatic interaction which hinders the oxidase-like catalytic activity of $ MnO_{2} $. Upon the introduction of target, the HEV target DNA binds with its complementary ssDNA on the surface of $ MnO_{2} $, the hybridized DNA releases from the surface of $ MnO_{2} $, which leads to recovery of oxidase-like catalytic activity of $ MnO_{2} $. This strategy was applied to construct a colorimetric technique for HEV detection. The approach works in the linear range of 1 fM–100 nM DNA concentration with the limit of detection (LOD) of 3.26 fM (S/N = 3) and quantitative limit (LOQ) of 36.08 fM. The TMB-$ MnO_{2} $ platform was highly selective for HEV target DNA detection when compared with potential interferences. Result of serum sample analysis demonstrates that this sensing system can be used for clinical diagnostic applications. Graphical Abstract Hepatitis E virus (dpeaa)DE-He213 MnO (dpeaa)DE-He213 Nanozymes (dpeaa)DE-He213 DNA (dpeaa)DE-He213 Oxidase-mimicking (dpeaa)DE-He213 Biosensors (dpeaa)DE-He213 Ravikumar, Chandan Hunsur aut Sreeramareddygari, Muralikrishna aut Somasundrum, Mithran aut Surareungchai, Werasak aut Enthalten in Analytical and bioanalytical chemistry Berlin : Springer, 2002 415(2022), 4 vom: 05. Dez., Seite 703-713 (DE-627)25372337X (DE-600)1459122-4 1618-2650 nnns volume:415 year:2022 number:4 day:05 month:12 pages:703-713 https://dx.doi.org/10.1007/s00216-022-04461-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 415 2022 4 05 12 703-713 |
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Enthalten in Analytical and bioanalytical chemistry 415(2022), 4 vom: 05. Dez., Seite 703-713 volume:415 year:2022 number:4 day:05 month:12 pages:703-713 |
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Alam, Naveed @@aut@@ Ravikumar, Chandan Hunsur @@aut@@ Sreeramareddygari, Muralikrishna @@aut@@ Somasundrum, Mithran @@aut@@ Surareungchai, Werasak @@aut@@ |
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Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Hepatitis E virus (HEV) is an evolving infectious entity that causes viral hepatitis infections worldwide. Current routine methods of identifying and diagnosing HEV are someway laborious and costly. Based on the biomimicking oxidase-like activity of $ MnO_{2} $ nanosheets, we designed a label-free, highly sensitive colorimetric sensing technique for HEV detection. The prepared $ MnO_{2} $ catalyst displays intrinsic biomimicking oxidase-like catalytic activity and efficiently oxidizes the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate from colorless to blue colored oxidized TMB (oxTMB) product which can be measured at 652 nm by UV–visible spectrum. When the HEV-DNA was added, DNA adsorbed easily on $ MnO_{2} $ surface through physical adsorption and electrostatic interaction which hinders the oxidase-like catalytic activity of $ MnO_{2} $. Upon the introduction of target, the HEV target DNA binds with its complementary ssDNA on the surface of $ MnO_{2} $, the hybridized DNA releases from the surface of $ MnO_{2} $, which leads to recovery of oxidase-like catalytic activity of $ MnO_{2} $. This strategy was applied to construct a colorimetric technique for HEV detection. The approach works in the linear range of 1 fM–100 nM DNA concentration with the limit of detection (LOD) of 3.26 fM (S/N = 3) and quantitative limit (LOQ) of 36.08 fM. The TMB-$ MnO_{2} $ platform was highly selective for HEV target DNA detection when compared with potential interferences. Result of serum sample analysis demonstrates that this sensing system can be used for clinical diagnostic applications. 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Alam, Naveed |
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Alam, Naveed misc Hepatitis E virus misc MnO misc Nanozymes misc DNA misc Oxidase-mimicking misc Biosensors Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of $ MnO_{2} $ nanosheets |
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Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of $ MnO_{2} $ nanosheets Hepatitis E virus (dpeaa)DE-He213 MnO (dpeaa)DE-He213 Nanozymes (dpeaa)DE-He213 DNA (dpeaa)DE-He213 Oxidase-mimicking (dpeaa)DE-He213 Biosensors (dpeaa)DE-He213 |
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misc Hepatitis E virus misc MnO misc Nanozymes misc DNA misc Oxidase-mimicking misc Biosensors |
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Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of $ MnO_{2} $ nanosheets |
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Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of $ MnO_{2} $ nanosheets |
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Alam, Naveed |
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Alam, Naveed Ravikumar, Chandan Hunsur Sreeramareddygari, Muralikrishna Somasundrum, Mithran Surareungchai, Werasak |
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10.1007/s00216-022-04461-1 |
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label-free ultra-sensitive colorimetric detection of hepatitis e virus based on oxidase-like activity of $ mno_{2} $ nanosheets |
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Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of $ MnO_{2} $ nanosheets |
| abstract |
Hepatitis E virus (HEV) is an evolving infectious entity that causes viral hepatitis infections worldwide. Current routine methods of identifying and diagnosing HEV are someway laborious and costly. Based on the biomimicking oxidase-like activity of $ MnO_{2} $ nanosheets, we designed a label-free, highly sensitive colorimetric sensing technique for HEV detection. The prepared $ MnO_{2} $ catalyst displays intrinsic biomimicking oxidase-like catalytic activity and efficiently oxidizes the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate from colorless to blue colored oxidized TMB (oxTMB) product which can be measured at 652 nm by UV–visible spectrum. When the HEV-DNA was added, DNA adsorbed easily on $ MnO_{2} $ surface through physical adsorption and electrostatic interaction which hinders the oxidase-like catalytic activity of $ MnO_{2} $. Upon the introduction of target, the HEV target DNA binds with its complementary ssDNA on the surface of $ MnO_{2} $, the hybridized DNA releases from the surface of $ MnO_{2} $, which leads to recovery of oxidase-like catalytic activity of $ MnO_{2} $. This strategy was applied to construct a colorimetric technique for HEV detection. The approach works in the linear range of 1 fM–100 nM DNA concentration with the limit of detection (LOD) of 3.26 fM (S/N = 3) and quantitative limit (LOQ) of 36.08 fM. The TMB-$ MnO_{2} $ platform was highly selective for HEV target DNA detection when compared with potential interferences. Result of serum sample analysis demonstrates that this sensing system can be used for clinical diagnostic applications. Graphical Abstract © Springer-Verlag GmbH Germany, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
| abstractGer |
Hepatitis E virus (HEV) is an evolving infectious entity that causes viral hepatitis infections worldwide. Current routine methods of identifying and diagnosing HEV are someway laborious and costly. Based on the biomimicking oxidase-like activity of $ MnO_{2} $ nanosheets, we designed a label-free, highly sensitive colorimetric sensing technique for HEV detection. The prepared $ MnO_{2} $ catalyst displays intrinsic biomimicking oxidase-like catalytic activity and efficiently oxidizes the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate from colorless to blue colored oxidized TMB (oxTMB) product which can be measured at 652 nm by UV–visible spectrum. When the HEV-DNA was added, DNA adsorbed easily on $ MnO_{2} $ surface through physical adsorption and electrostatic interaction which hinders the oxidase-like catalytic activity of $ MnO_{2} $. Upon the introduction of target, the HEV target DNA binds with its complementary ssDNA on the surface of $ MnO_{2} $, the hybridized DNA releases from the surface of $ MnO_{2} $, which leads to recovery of oxidase-like catalytic activity of $ MnO_{2} $. This strategy was applied to construct a colorimetric technique for HEV detection. The approach works in the linear range of 1 fM–100 nM DNA concentration with the limit of detection (LOD) of 3.26 fM (S/N = 3) and quantitative limit (LOQ) of 36.08 fM. The TMB-$ MnO_{2} $ platform was highly selective for HEV target DNA detection when compared with potential interferences. Result of serum sample analysis demonstrates that this sensing system can be used for clinical diagnostic applications. Graphical Abstract © Springer-Verlag GmbH Germany, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
| abstract_unstemmed |
Hepatitis E virus (HEV) is an evolving infectious entity that causes viral hepatitis infections worldwide. Current routine methods of identifying and diagnosing HEV are someway laborious and costly. Based on the biomimicking oxidase-like activity of $ MnO_{2} $ nanosheets, we designed a label-free, highly sensitive colorimetric sensing technique for HEV detection. The prepared $ MnO_{2} $ catalyst displays intrinsic biomimicking oxidase-like catalytic activity and efficiently oxidizes the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate from colorless to blue colored oxidized TMB (oxTMB) product which can be measured at 652 nm by UV–visible spectrum. When the HEV-DNA was added, DNA adsorbed easily on $ MnO_{2} $ surface through physical adsorption and electrostatic interaction which hinders the oxidase-like catalytic activity of $ MnO_{2} $. Upon the introduction of target, the HEV target DNA binds with its complementary ssDNA on the surface of $ MnO_{2} $, the hybridized DNA releases from the surface of $ MnO_{2} $, which leads to recovery of oxidase-like catalytic activity of $ MnO_{2} $. This strategy was applied to construct a colorimetric technique for HEV detection. The approach works in the linear range of 1 fM–100 nM DNA concentration with the limit of detection (LOD) of 3.26 fM (S/N = 3) and quantitative limit (LOQ) of 36.08 fM. The TMB-$ MnO_{2} $ platform was highly selective for HEV target DNA detection when compared with potential interferences. Result of serum sample analysis demonstrates that this sensing system can be used for clinical diagnostic applications. Graphical Abstract © Springer-Verlag GmbH Germany, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| container_issue |
4 |
| title_short |
Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of $ MnO_{2} $ nanosheets |
| url |
https://dx.doi.org/10.1007/s00216-022-04461-1 |
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| author2 |
Ravikumar, Chandan Hunsur Sreeramareddygari, Muralikrishna Somasundrum, Mithran Surareungchai, Werasak |
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Ravikumar, Chandan Hunsur Sreeramareddygari, Muralikrishna Somasundrum, Mithran Surareungchai, Werasak |
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| doi_str |
10.1007/s00216-022-04461-1 |
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2024-07-04T00:43:38.977Z |
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| score |
7.401986 |