Stability of pollen sterility and characterization of Cajanus scarabaeoides (L.) Thouars ($ A_{2} $) based male sterile lines in pigeonpea (Cajanus cajan (L.) Millsp.)
Abstract The study on stability of pollen sterility of three $ A_{2} $ cytoplasm based male sterile lines (CORG 990047A, CORG 990052A and CORG 7A) showed that all the three male sterile lines had highly stable pollen sterility over 27 fortnight intervals. The various studies on pollen fertility, ant...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Ranjani, M. S. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022 |
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| Schlagwörter: |
Cytoplasmic genetic male sterile system |
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| Anmerkung: |
© The Author(s), under exclusive licence to Botanical Society of Sao Paulo 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| Übergeordnetes Werk: |
Enthalten in: Brazilian journal of botany - São Paulo : Springer, 2012, 46(2022), 1 vom: 19. Dez., Seite 163-175 |
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| Übergeordnetes Werk: |
volume:46 ; year:2022 ; number:1 ; day:19 ; month:12 ; pages:163-175 |
| Links: |
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| DOI / URN: |
10.1007/s40415-022-00859-4 |
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| Katalog-ID: |
SPR049510339 |
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| 100 | 1 | |a Ranjani, M. S. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Stability of pollen sterility and characterization of Cajanus scarabaeoides (L.) Thouars ($ A_{2} $) based male sterile lines in pigeonpea (Cajanus cajan (L.) Millsp.) |
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| 520 | |a Abstract The study on stability of pollen sterility of three $ A_{2} $ cytoplasm based male sterile lines (CORG 990047A, CORG 990052A and CORG 7A) showed that all the three male sterile lines had highly stable pollen sterility over 27 fortnight intervals. The various studies on pollen fertility, anther size and scanning electron microscopic image analysis revealed a clear insight on the anthers and pollen grain morphology of A and B lines. The nad7a_del marker distinguished the A and B lines of $ A_{4} $ cytoplasm based male sterile lines by the allele size of 150 (10 bp deletion) and 160 bp, respectively. However, deletion of 10 bp was not observed in the A lines of $ A_{2} $ cytoplasm (160 bp). Hence, this study proved that the nd7a_del marker can be used to differentiate $ A_{2} $ and $ A_{4} $ cytoplasm based male sterile lines. DNA fingerprinting study revealed that 12 SSR markers were polymorphic among the 47 markers used and two SSR markers viz., CcLG08_RFQI4 and CCM1277 were polymorphic and differentiated all the three-male sterile lines. These markers could be used in DNA fingerprinting of the A lines and to maintain their genetic purity. | ||
| 650 | 4 | |a Cytoplasmic genetic male sterile system |7 (dpeaa)DE-He213 | |
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| 650 | 4 | |a Morphological and molecular characterization |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Pigeonpea |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Stability of pollen sterility |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Scanning Electron Microscope |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Jayamani, P. |4 aut | |
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10.1007/s40415-022-00859-4 doi (DE-627)SPR049510339 (SPR)s40415-022-00859-4-e DE-627 ger DE-627 rakwb eng Ranjani, M. S. verfasserin aut Stability of pollen sterility and characterization of Cajanus scarabaeoides (L.) Thouars ($ A_{2} $) based male sterile lines in pigeonpea (Cajanus cajan (L.) Millsp.) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Botanical Society of Sao Paulo 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract The study on stability of pollen sterility of three $ A_{2} $ cytoplasm based male sterile lines (CORG 990047A, CORG 990052A and CORG 7A) showed that all the three male sterile lines had highly stable pollen sterility over 27 fortnight intervals. The various studies on pollen fertility, anther size and scanning electron microscopic image analysis revealed a clear insight on the anthers and pollen grain morphology of A and B lines. The nad7a_del marker distinguished the A and B lines of $ A_{4} $ cytoplasm based male sterile lines by the allele size of 150 (10 bp deletion) and 160 bp, respectively. However, deletion of 10 bp was not observed in the A lines of $ A_{2} $ cytoplasm (160 bp). Hence, this study proved that the nd7a_del marker can be used to differentiate $ A_{2} $ and $ A_{4} $ cytoplasm based male sterile lines. DNA fingerprinting study revealed that 12 SSR markers were polymorphic among the 47 markers used and two SSR markers viz., CcLG08_RFQI4 and CCM1277 were polymorphic and differentiated all the three-male sterile lines. These markers could be used in DNA fingerprinting of the A lines and to maintain their genetic purity. Cytoplasmic genetic male sterile system (dpeaa)DE-He213 DNA fingerprinting (dpeaa)DE-He213 Morphological and molecular characterization (dpeaa)DE-He213 Pigeonpea (dpeaa)DE-He213 Stability of pollen sterility (dpeaa)DE-He213 Scanning Electron Microscope (dpeaa)DE-He213 Jayamani, P. aut Enthalten in Brazilian journal of botany São Paulo : Springer, 2012 46(2022), 1 vom: 19. Dez., Seite 163-175 (DE-627)727814389 (DE-600)2686406-X 1806-9959 nnns volume:46 year:2022 number:1 day:19 month:12 pages:163-175 https://dx.doi.org/10.1007/s40415-022-00859-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 46 2022 1 19 12 163-175 |
| spelling |
10.1007/s40415-022-00859-4 doi (DE-627)SPR049510339 (SPR)s40415-022-00859-4-e DE-627 ger DE-627 rakwb eng Ranjani, M. S. verfasserin aut Stability of pollen sterility and characterization of Cajanus scarabaeoides (L.) Thouars ($ A_{2} $) based male sterile lines in pigeonpea (Cajanus cajan (L.) Millsp.) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Botanical Society of Sao Paulo 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract The study on stability of pollen sterility of three $ A_{2} $ cytoplasm based male sterile lines (CORG 990047A, CORG 990052A and CORG 7A) showed that all the three male sterile lines had highly stable pollen sterility over 27 fortnight intervals. The various studies on pollen fertility, anther size and scanning electron microscopic image analysis revealed a clear insight on the anthers and pollen grain morphology of A and B lines. The nad7a_del marker distinguished the A and B lines of $ A_{4} $ cytoplasm based male sterile lines by the allele size of 150 (10 bp deletion) and 160 bp, respectively. However, deletion of 10 bp was not observed in the A lines of $ A_{2} $ cytoplasm (160 bp). Hence, this study proved that the nd7a_del marker can be used to differentiate $ A_{2} $ and $ A_{4} $ cytoplasm based male sterile lines. DNA fingerprinting study revealed that 12 SSR markers were polymorphic among the 47 markers used and two SSR markers viz., CcLG08_RFQI4 and CCM1277 were polymorphic and differentiated all the three-male sterile lines. These markers could be used in DNA fingerprinting of the A lines and to maintain their genetic purity. Cytoplasmic genetic male sterile system (dpeaa)DE-He213 DNA fingerprinting (dpeaa)DE-He213 Morphological and molecular characterization (dpeaa)DE-He213 Pigeonpea (dpeaa)DE-He213 Stability of pollen sterility (dpeaa)DE-He213 Scanning Electron Microscope (dpeaa)DE-He213 Jayamani, P. aut Enthalten in Brazilian journal of botany São Paulo : Springer, 2012 46(2022), 1 vom: 19. Dez., Seite 163-175 (DE-627)727814389 (DE-600)2686406-X 1806-9959 nnns volume:46 year:2022 number:1 day:19 month:12 pages:163-175 https://dx.doi.org/10.1007/s40415-022-00859-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 46 2022 1 19 12 163-175 |
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10.1007/s40415-022-00859-4 doi (DE-627)SPR049510339 (SPR)s40415-022-00859-4-e DE-627 ger DE-627 rakwb eng Ranjani, M. S. verfasserin aut Stability of pollen sterility and characterization of Cajanus scarabaeoides (L.) Thouars ($ A_{2} $) based male sterile lines in pigeonpea (Cajanus cajan (L.) Millsp.) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Botanical Society of Sao Paulo 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract The study on stability of pollen sterility of three $ A_{2} $ cytoplasm based male sterile lines (CORG 990047A, CORG 990052A and CORG 7A) showed that all the three male sterile lines had highly stable pollen sterility over 27 fortnight intervals. The various studies on pollen fertility, anther size and scanning electron microscopic image analysis revealed a clear insight on the anthers and pollen grain morphology of A and B lines. The nad7a_del marker distinguished the A and B lines of $ A_{4} $ cytoplasm based male sterile lines by the allele size of 150 (10 bp deletion) and 160 bp, respectively. However, deletion of 10 bp was not observed in the A lines of $ A_{2} $ cytoplasm (160 bp). Hence, this study proved that the nd7a_del marker can be used to differentiate $ A_{2} $ and $ A_{4} $ cytoplasm based male sterile lines. DNA fingerprinting study revealed that 12 SSR markers were polymorphic among the 47 markers used and two SSR markers viz., CcLG08_RFQI4 and CCM1277 were polymorphic and differentiated all the three-male sterile lines. These markers could be used in DNA fingerprinting of the A lines and to maintain their genetic purity. Cytoplasmic genetic male sterile system (dpeaa)DE-He213 DNA fingerprinting (dpeaa)DE-He213 Morphological and molecular characterization (dpeaa)DE-He213 Pigeonpea (dpeaa)DE-He213 Stability of pollen sterility (dpeaa)DE-He213 Scanning Electron Microscope (dpeaa)DE-He213 Jayamani, P. aut Enthalten in Brazilian journal of botany São Paulo : Springer, 2012 46(2022), 1 vom: 19. Dez., Seite 163-175 (DE-627)727814389 (DE-600)2686406-X 1806-9959 nnns volume:46 year:2022 number:1 day:19 month:12 pages:163-175 https://dx.doi.org/10.1007/s40415-022-00859-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 46 2022 1 19 12 163-175 |
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10.1007/s40415-022-00859-4 doi (DE-627)SPR049510339 (SPR)s40415-022-00859-4-e DE-627 ger DE-627 rakwb eng Ranjani, M. S. verfasserin aut Stability of pollen sterility and characterization of Cajanus scarabaeoides (L.) Thouars ($ A_{2} $) based male sterile lines in pigeonpea (Cajanus cajan (L.) Millsp.) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Botanical Society of Sao Paulo 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract The study on stability of pollen sterility of three $ A_{2} $ cytoplasm based male sterile lines (CORG 990047A, CORG 990052A and CORG 7A) showed that all the three male sterile lines had highly stable pollen sterility over 27 fortnight intervals. The various studies on pollen fertility, anther size and scanning electron microscopic image analysis revealed a clear insight on the anthers and pollen grain morphology of A and B lines. The nad7a_del marker distinguished the A and B lines of $ A_{4} $ cytoplasm based male sterile lines by the allele size of 150 (10 bp deletion) and 160 bp, respectively. However, deletion of 10 bp was not observed in the A lines of $ A_{2} $ cytoplasm (160 bp). Hence, this study proved that the nd7a_del marker can be used to differentiate $ A_{2} $ and $ A_{4} $ cytoplasm based male sterile lines. DNA fingerprinting study revealed that 12 SSR markers were polymorphic among the 47 markers used and two SSR markers viz., CcLG08_RFQI4 and CCM1277 were polymorphic and differentiated all the three-male sterile lines. These markers could be used in DNA fingerprinting of the A lines and to maintain their genetic purity. Cytoplasmic genetic male sterile system (dpeaa)DE-He213 DNA fingerprinting (dpeaa)DE-He213 Morphological and molecular characterization (dpeaa)DE-He213 Pigeonpea (dpeaa)DE-He213 Stability of pollen sterility (dpeaa)DE-He213 Scanning Electron Microscope (dpeaa)DE-He213 Jayamani, P. aut Enthalten in Brazilian journal of botany São Paulo : Springer, 2012 46(2022), 1 vom: 19. Dez., Seite 163-175 (DE-627)727814389 (DE-600)2686406-X 1806-9959 nnns volume:46 year:2022 number:1 day:19 month:12 pages:163-175 https://dx.doi.org/10.1007/s40415-022-00859-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 46 2022 1 19 12 163-175 |
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10.1007/s40415-022-00859-4 doi (DE-627)SPR049510339 (SPR)s40415-022-00859-4-e DE-627 ger DE-627 rakwb eng Ranjani, M. S. verfasserin aut Stability of pollen sterility and characterization of Cajanus scarabaeoides (L.) Thouars ($ A_{2} $) based male sterile lines in pigeonpea (Cajanus cajan (L.) Millsp.) 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Botanical Society of Sao Paulo 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract The study on stability of pollen sterility of three $ A_{2} $ cytoplasm based male sterile lines (CORG 990047A, CORG 990052A and CORG 7A) showed that all the three male sterile lines had highly stable pollen sterility over 27 fortnight intervals. The various studies on pollen fertility, anther size and scanning electron microscopic image analysis revealed a clear insight on the anthers and pollen grain morphology of A and B lines. The nad7a_del marker distinguished the A and B lines of $ A_{4} $ cytoplasm based male sterile lines by the allele size of 150 (10 bp deletion) and 160 bp, respectively. However, deletion of 10 bp was not observed in the A lines of $ A_{2} $ cytoplasm (160 bp). Hence, this study proved that the nd7a_del marker can be used to differentiate $ A_{2} $ and $ A_{4} $ cytoplasm based male sterile lines. DNA fingerprinting study revealed that 12 SSR markers were polymorphic among the 47 markers used and two SSR markers viz., CcLG08_RFQI4 and CCM1277 were polymorphic and differentiated all the three-male sterile lines. These markers could be used in DNA fingerprinting of the A lines and to maintain their genetic purity. Cytoplasmic genetic male sterile system (dpeaa)DE-He213 DNA fingerprinting (dpeaa)DE-He213 Morphological and molecular characterization (dpeaa)DE-He213 Pigeonpea (dpeaa)DE-He213 Stability of pollen sterility (dpeaa)DE-He213 Scanning Electron Microscope (dpeaa)DE-He213 Jayamani, P. aut Enthalten in Brazilian journal of botany São Paulo : Springer, 2012 46(2022), 1 vom: 19. Dez., Seite 163-175 (DE-627)727814389 (DE-600)2686406-X 1806-9959 nnns volume:46 year:2022 number:1 day:19 month:12 pages:163-175 https://dx.doi.org/10.1007/s40415-022-00859-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 46 2022 1 19 12 163-175 |
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Ranjani, M. S. |
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Ranjani, M. S. misc Cytoplasmic genetic male sterile system misc DNA fingerprinting misc Morphological and molecular characterization misc Pigeonpea misc Stability of pollen sterility misc Scanning Electron Microscope Stability of pollen sterility and characterization of Cajanus scarabaeoides (L.) Thouars ($ A_{2} $) based male sterile lines in pigeonpea (Cajanus cajan (L.) Millsp.) |
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Stability of pollen sterility and characterization of Cajanus scarabaeoides (L.) Thouars ($ A_{2} $) based male sterile lines in pigeonpea (Cajanus cajan (L.) Millsp.) Cytoplasmic genetic male sterile system (dpeaa)DE-He213 DNA fingerprinting (dpeaa)DE-He213 Morphological and molecular characterization (dpeaa)DE-He213 Pigeonpea (dpeaa)DE-He213 Stability of pollen sterility (dpeaa)DE-He213 Scanning Electron Microscope (dpeaa)DE-He213 |
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misc Cytoplasmic genetic male sterile system misc DNA fingerprinting misc Morphological and molecular characterization misc Pigeonpea misc Stability of pollen sterility misc Scanning Electron Microscope |
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Stability of pollen sterility and characterization of Cajanus scarabaeoides (L.) Thouars ($ A_{2} $) based male sterile lines in pigeonpea (Cajanus cajan (L.) Millsp.) |
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Stability of pollen sterility and characterization of Cajanus scarabaeoides (L.) Thouars ($ A_{2} $) based male sterile lines in pigeonpea (Cajanus cajan (L.) Millsp.) |
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stability of pollen sterility and characterization of cajanus scarabaeoides (l.) thouars ($ a_{2} $) based male sterile lines in pigeonpea (cajanus cajan (l.) millsp.) |
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Stability of pollen sterility and characterization of Cajanus scarabaeoides (L.) Thouars ($ A_{2} $) based male sterile lines in pigeonpea (Cajanus cajan (L.) Millsp.) |
| abstract |
Abstract The study on stability of pollen sterility of three $ A_{2} $ cytoplasm based male sterile lines (CORG 990047A, CORG 990052A and CORG 7A) showed that all the three male sterile lines had highly stable pollen sterility over 27 fortnight intervals. The various studies on pollen fertility, anther size and scanning electron microscopic image analysis revealed a clear insight on the anthers and pollen grain morphology of A and B lines. The nad7a_del marker distinguished the A and B lines of $ A_{4} $ cytoplasm based male sterile lines by the allele size of 150 (10 bp deletion) and 160 bp, respectively. However, deletion of 10 bp was not observed in the A lines of $ A_{2} $ cytoplasm (160 bp). Hence, this study proved that the nd7a_del marker can be used to differentiate $ A_{2} $ and $ A_{4} $ cytoplasm based male sterile lines. DNA fingerprinting study revealed that 12 SSR markers were polymorphic among the 47 markers used and two SSR markers viz., CcLG08_RFQI4 and CCM1277 were polymorphic and differentiated all the three-male sterile lines. These markers could be used in DNA fingerprinting of the A lines and to maintain their genetic purity. © The Author(s), under exclusive licence to Botanical Society of Sao Paulo 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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Abstract The study on stability of pollen sterility of three $ A_{2} $ cytoplasm based male sterile lines (CORG 990047A, CORG 990052A and CORG 7A) showed that all the three male sterile lines had highly stable pollen sterility over 27 fortnight intervals. The various studies on pollen fertility, anther size and scanning electron microscopic image analysis revealed a clear insight on the anthers and pollen grain morphology of A and B lines. The nad7a_del marker distinguished the A and B lines of $ A_{4} $ cytoplasm based male sterile lines by the allele size of 150 (10 bp deletion) and 160 bp, respectively. However, deletion of 10 bp was not observed in the A lines of $ A_{2} $ cytoplasm (160 bp). Hence, this study proved that the nd7a_del marker can be used to differentiate $ A_{2} $ and $ A_{4} $ cytoplasm based male sterile lines. DNA fingerprinting study revealed that 12 SSR markers were polymorphic among the 47 markers used and two SSR markers viz., CcLG08_RFQI4 and CCM1277 were polymorphic and differentiated all the three-male sterile lines. These markers could be used in DNA fingerprinting of the A lines and to maintain their genetic purity. © The Author(s), under exclusive licence to Botanical Society of Sao Paulo 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
| abstract_unstemmed |
Abstract The study on stability of pollen sterility of three $ A_{2} $ cytoplasm based male sterile lines (CORG 990047A, CORG 990052A and CORG 7A) showed that all the three male sterile lines had highly stable pollen sterility over 27 fortnight intervals. The various studies on pollen fertility, anther size and scanning electron microscopic image analysis revealed a clear insight on the anthers and pollen grain morphology of A and B lines. The nad7a_del marker distinguished the A and B lines of $ A_{4} $ cytoplasm based male sterile lines by the allele size of 150 (10 bp deletion) and 160 bp, respectively. However, deletion of 10 bp was not observed in the A lines of $ A_{2} $ cytoplasm (160 bp). Hence, this study proved that the nd7a_del marker can be used to differentiate $ A_{2} $ and $ A_{4} $ cytoplasm based male sterile lines. DNA fingerprinting study revealed that 12 SSR markers were polymorphic among the 47 markers used and two SSR markers viz., CcLG08_RFQI4 and CCM1277 were polymorphic and differentiated all the three-male sterile lines. These markers could be used in DNA fingerprinting of the A lines and to maintain their genetic purity. © The Author(s), under exclusive licence to Botanical Society of Sao Paulo 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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Stability of pollen sterility and characterization of Cajanus scarabaeoides (L.) Thouars ($ A_{2} $) based male sterile lines in pigeonpea (Cajanus cajan (L.) Millsp.) |
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https://dx.doi.org/10.1007/s40415-022-00859-4 |
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Jayamani, P. |
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10.1007/s40415-022-00859-4 |
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2024-07-04T01:06:58.149Z |
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| score |
7.4017773 |