Serratia silvae sp. nov., Isolated from Forest Soil
Abstract The Gram-negative, oxidase-negative, catalase-positive, rod-shaped strain $ Arafor3^{T} $ was isolated from forest soil (France). Comparative 16S rRNA gene analysis and phylogenetic analysis based on (1) multilocus sequence analysis (MLSA) with four housekeeping genes (atpD, gyrB, infB and...
Ausführliche Beschreibung
Autor*in: |
Cho, Gyu-Sung [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2023 |
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Anmerkung: |
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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Übergeordnetes Werk: |
Enthalten in: Current microbiology - New York, NY : Springer, 1978, 80(2023), 4 vom: 24. Feb. |
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Übergeordnetes Werk: |
volume:80 ; year:2023 ; number:4 ; day:24 ; month:02 |
Links: |
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DOI / URN: |
10.1007/s00284-023-03214-2 |
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Katalog-ID: |
SPR049718304 |
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520 | |a Abstract The Gram-negative, oxidase-negative, catalase-positive, rod-shaped strain $ Arafor3^{T} $ was isolated from forest soil (France). Comparative 16S rRNA gene analysis and phylogenetic analysis based on (1) multilocus sequence analysis (MLSA) with four housekeeping genes (atpD, gyrB, infB and rpoB) and (2) genomes indicated that strain $ Arafor3^{T} $ shared 98.83% 16S rRNA gene sequence similarity with the type strain of Serratia fonticola DSM $ 4576^{T} $ and was closely related to this same strain in the MLSA and in the phylogenomic tree reconstruction. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) comparisons of strain $ Arafor3^{T} $ with its nearest neighbor S. fonticola DSM $ 4576^{T} $ showed 93.5% identity and 55.7% sequence similarity, respectively, and were lower than the 96% and 70% species-level cut-off values relating to these analyses (Logan et al. in Int J Syst Evol Microbiol 59:2114–21, 2009, https://doi.org/10.1099/ijs.0.013649-0). The strain differed from S. fonticola in that it was urease and arginine dihydrolase negative. The major fatty acids of strain $ Arafor3^{T} $ are $ C_{16:0} $, $ C_{16:1} $ω7c/$ C_{16:1} $ω6c, C14:0, $ C_{14:0} $ 3-OH/16:1 isoI, and $ C_{18:1} $ω7c. The major respiratory quinone is Q8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and 6 unknown lipids. The mol G + C% content of the genomic DNA of strain $ Arafor3^{T} $ was 53.49%. Hence, $ Arafor3^{T} $ represents a novel species within the genus Serratia, for which the name Serratia silvae sp. nov. is proposed. The type strain is $ Arafor3^{T} $ (=LMG $ 32338^{T} $ = CIP $ 111939^{T} $). | ||
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700 | 1 | |a Besaury, Ludovic |0 (orcid)0000-0002-1463-1239 |4 aut | |
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10.1007/s00284-023-03214-2 doi (DE-627)SPR049718304 (SPR)s00284-023-03214-2-e DE-627 ger DE-627 rakwb eng Cho, Gyu-Sung verfasserin aut Serratia silvae sp. nov., Isolated from Forest Soil 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract The Gram-negative, oxidase-negative, catalase-positive, rod-shaped strain $ Arafor3^{T} $ was isolated from forest soil (France). Comparative 16S rRNA gene analysis and phylogenetic analysis based on (1) multilocus sequence analysis (MLSA) with four housekeeping genes (atpD, gyrB, infB and rpoB) and (2) genomes indicated that strain $ Arafor3^{T} $ shared 98.83% 16S rRNA gene sequence similarity with the type strain of Serratia fonticola DSM $ 4576^{T} $ and was closely related to this same strain in the MLSA and in the phylogenomic tree reconstruction. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) comparisons of strain $ Arafor3^{T} $ with its nearest neighbor S. fonticola DSM $ 4576^{T} $ showed 93.5% identity and 55.7% sequence similarity, respectively, and were lower than the 96% and 70% species-level cut-off values relating to these analyses (Logan et al. in Int J Syst Evol Microbiol 59:2114–21, 2009, https://doi.org/10.1099/ijs.0.013649-0). The strain differed from S. fonticola in that it was urease and arginine dihydrolase negative. The major fatty acids of strain $ Arafor3^{T} $ are $ C_{16:0} $, $ C_{16:1} $ω7c/$ C_{16:1} $ω6c, C14:0, $ C_{14:0} $ 3-OH/16:1 isoI, and $ C_{18:1} $ω7c. The major respiratory quinone is Q8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and 6 unknown lipids. The mol G + C% content of the genomic DNA of strain $ Arafor3^{T} $ was 53.49%. Hence, $ Arafor3^{T} $ represents a novel species within the genus Serratia, for which the name Serratia silvae sp. nov. is proposed. The type strain is $ Arafor3^{T} $ (=LMG $ 32338^{T} $ = CIP $ 111939^{T} $). Mühle, Estelle aut Clermont, Dominique aut Franz, Charles M. A. P. aut Besaury, Ludovic (orcid)0000-0002-1463-1239 aut Enthalten in Current microbiology New York, NY : Springer, 1978 80(2023), 4 vom: 24. Feb. (DE-627)253722160 (DE-600)1458987-4 1432-0991 nnns volume:80 year:2023 number:4 day:24 month:02 https://dx.doi.org/10.1007/s00284-023-03214-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 80 2023 4 24 02 |
spelling |
10.1007/s00284-023-03214-2 doi (DE-627)SPR049718304 (SPR)s00284-023-03214-2-e DE-627 ger DE-627 rakwb eng Cho, Gyu-Sung verfasserin aut Serratia silvae sp. nov., Isolated from Forest Soil 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract The Gram-negative, oxidase-negative, catalase-positive, rod-shaped strain $ Arafor3^{T} $ was isolated from forest soil (France). Comparative 16S rRNA gene analysis and phylogenetic analysis based on (1) multilocus sequence analysis (MLSA) with four housekeeping genes (atpD, gyrB, infB and rpoB) and (2) genomes indicated that strain $ Arafor3^{T} $ shared 98.83% 16S rRNA gene sequence similarity with the type strain of Serratia fonticola DSM $ 4576^{T} $ and was closely related to this same strain in the MLSA and in the phylogenomic tree reconstruction. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) comparisons of strain $ Arafor3^{T} $ with its nearest neighbor S. fonticola DSM $ 4576^{T} $ showed 93.5% identity and 55.7% sequence similarity, respectively, and were lower than the 96% and 70% species-level cut-off values relating to these analyses (Logan et al. in Int J Syst Evol Microbiol 59:2114–21, 2009, https://doi.org/10.1099/ijs.0.013649-0). The strain differed from S. fonticola in that it was urease and arginine dihydrolase negative. The major fatty acids of strain $ Arafor3^{T} $ are $ C_{16:0} $, $ C_{16:1} $ω7c/$ C_{16:1} $ω6c, C14:0, $ C_{14:0} $ 3-OH/16:1 isoI, and $ C_{18:1} $ω7c. The major respiratory quinone is Q8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and 6 unknown lipids. The mol G + C% content of the genomic DNA of strain $ Arafor3^{T} $ was 53.49%. Hence, $ Arafor3^{T} $ represents a novel species within the genus Serratia, for which the name Serratia silvae sp. nov. is proposed. The type strain is $ Arafor3^{T} $ (=LMG $ 32338^{T} $ = CIP $ 111939^{T} $). Mühle, Estelle aut Clermont, Dominique aut Franz, Charles M. A. P. aut Besaury, Ludovic (orcid)0000-0002-1463-1239 aut Enthalten in Current microbiology New York, NY : Springer, 1978 80(2023), 4 vom: 24. Feb. (DE-627)253722160 (DE-600)1458987-4 1432-0991 nnns volume:80 year:2023 number:4 day:24 month:02 https://dx.doi.org/10.1007/s00284-023-03214-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 80 2023 4 24 02 |
allfields_unstemmed |
10.1007/s00284-023-03214-2 doi (DE-627)SPR049718304 (SPR)s00284-023-03214-2-e DE-627 ger DE-627 rakwb eng Cho, Gyu-Sung verfasserin aut Serratia silvae sp. nov., Isolated from Forest Soil 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract The Gram-negative, oxidase-negative, catalase-positive, rod-shaped strain $ Arafor3^{T} $ was isolated from forest soil (France). Comparative 16S rRNA gene analysis and phylogenetic analysis based on (1) multilocus sequence analysis (MLSA) with four housekeeping genes (atpD, gyrB, infB and rpoB) and (2) genomes indicated that strain $ Arafor3^{T} $ shared 98.83% 16S rRNA gene sequence similarity with the type strain of Serratia fonticola DSM $ 4576^{T} $ and was closely related to this same strain in the MLSA and in the phylogenomic tree reconstruction. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) comparisons of strain $ Arafor3^{T} $ with its nearest neighbor S. fonticola DSM $ 4576^{T} $ showed 93.5% identity and 55.7% sequence similarity, respectively, and were lower than the 96% and 70% species-level cut-off values relating to these analyses (Logan et al. in Int J Syst Evol Microbiol 59:2114–21, 2009, https://doi.org/10.1099/ijs.0.013649-0). The strain differed from S. fonticola in that it was urease and arginine dihydrolase negative. The major fatty acids of strain $ Arafor3^{T} $ are $ C_{16:0} $, $ C_{16:1} $ω7c/$ C_{16:1} $ω6c, C14:0, $ C_{14:0} $ 3-OH/16:1 isoI, and $ C_{18:1} $ω7c. The major respiratory quinone is Q8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and 6 unknown lipids. The mol G + C% content of the genomic DNA of strain $ Arafor3^{T} $ was 53.49%. Hence, $ Arafor3^{T} $ represents a novel species within the genus Serratia, for which the name Serratia silvae sp. nov. is proposed. The type strain is $ Arafor3^{T} $ (=LMG $ 32338^{T} $ = CIP $ 111939^{T} $). Mühle, Estelle aut Clermont, Dominique aut Franz, Charles M. A. P. aut Besaury, Ludovic (orcid)0000-0002-1463-1239 aut Enthalten in Current microbiology New York, NY : Springer, 1978 80(2023), 4 vom: 24. Feb. (DE-627)253722160 (DE-600)1458987-4 1432-0991 nnns volume:80 year:2023 number:4 day:24 month:02 https://dx.doi.org/10.1007/s00284-023-03214-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 80 2023 4 24 02 |
allfieldsGer |
10.1007/s00284-023-03214-2 doi (DE-627)SPR049718304 (SPR)s00284-023-03214-2-e DE-627 ger DE-627 rakwb eng Cho, Gyu-Sung verfasserin aut Serratia silvae sp. nov., Isolated from Forest Soil 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract The Gram-negative, oxidase-negative, catalase-positive, rod-shaped strain $ Arafor3^{T} $ was isolated from forest soil (France). Comparative 16S rRNA gene analysis and phylogenetic analysis based on (1) multilocus sequence analysis (MLSA) with four housekeeping genes (atpD, gyrB, infB and rpoB) and (2) genomes indicated that strain $ Arafor3^{T} $ shared 98.83% 16S rRNA gene sequence similarity with the type strain of Serratia fonticola DSM $ 4576^{T} $ and was closely related to this same strain in the MLSA and in the phylogenomic tree reconstruction. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) comparisons of strain $ Arafor3^{T} $ with its nearest neighbor S. fonticola DSM $ 4576^{T} $ showed 93.5% identity and 55.7% sequence similarity, respectively, and were lower than the 96% and 70% species-level cut-off values relating to these analyses (Logan et al. in Int J Syst Evol Microbiol 59:2114–21, 2009, https://doi.org/10.1099/ijs.0.013649-0). The strain differed from S. fonticola in that it was urease and arginine dihydrolase negative. The major fatty acids of strain $ Arafor3^{T} $ are $ C_{16:0} $, $ C_{16:1} $ω7c/$ C_{16:1} $ω6c, C14:0, $ C_{14:0} $ 3-OH/16:1 isoI, and $ C_{18:1} $ω7c. The major respiratory quinone is Q8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and 6 unknown lipids. The mol G + C% content of the genomic DNA of strain $ Arafor3^{T} $ was 53.49%. Hence, $ Arafor3^{T} $ represents a novel species within the genus Serratia, for which the name Serratia silvae sp. nov. is proposed. The type strain is $ Arafor3^{T} $ (=LMG $ 32338^{T} $ = CIP $ 111939^{T} $). Mühle, Estelle aut Clermont, Dominique aut Franz, Charles M. A. P. aut Besaury, Ludovic (orcid)0000-0002-1463-1239 aut Enthalten in Current microbiology New York, NY : Springer, 1978 80(2023), 4 vom: 24. Feb. (DE-627)253722160 (DE-600)1458987-4 1432-0991 nnns volume:80 year:2023 number:4 day:24 month:02 https://dx.doi.org/10.1007/s00284-023-03214-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 80 2023 4 24 02 |
allfieldsSound |
10.1007/s00284-023-03214-2 doi (DE-627)SPR049718304 (SPR)s00284-023-03214-2-e DE-627 ger DE-627 rakwb eng Cho, Gyu-Sung verfasserin aut Serratia silvae sp. nov., Isolated from Forest Soil 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract The Gram-negative, oxidase-negative, catalase-positive, rod-shaped strain $ Arafor3^{T} $ was isolated from forest soil (France). Comparative 16S rRNA gene analysis and phylogenetic analysis based on (1) multilocus sequence analysis (MLSA) with four housekeeping genes (atpD, gyrB, infB and rpoB) and (2) genomes indicated that strain $ Arafor3^{T} $ shared 98.83% 16S rRNA gene sequence similarity with the type strain of Serratia fonticola DSM $ 4576^{T} $ and was closely related to this same strain in the MLSA and in the phylogenomic tree reconstruction. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) comparisons of strain $ Arafor3^{T} $ with its nearest neighbor S. fonticola DSM $ 4576^{T} $ showed 93.5% identity and 55.7% sequence similarity, respectively, and were lower than the 96% and 70% species-level cut-off values relating to these analyses (Logan et al. in Int J Syst Evol Microbiol 59:2114–21, 2009, https://doi.org/10.1099/ijs.0.013649-0). The strain differed from S. fonticola in that it was urease and arginine dihydrolase negative. The major fatty acids of strain $ Arafor3^{T} $ are $ C_{16:0} $, $ C_{16:1} $ω7c/$ C_{16:1} $ω6c, C14:0, $ C_{14:0} $ 3-OH/16:1 isoI, and $ C_{18:1} $ω7c. The major respiratory quinone is Q8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and 6 unknown lipids. The mol G + C% content of the genomic DNA of strain $ Arafor3^{T} $ was 53.49%. Hence, $ Arafor3^{T} $ represents a novel species within the genus Serratia, for which the name Serratia silvae sp. nov. is proposed. The type strain is $ Arafor3^{T} $ (=LMG $ 32338^{T} $ = CIP $ 111939^{T} $). Mühle, Estelle aut Clermont, Dominique aut Franz, Charles M. A. P. aut Besaury, Ludovic (orcid)0000-0002-1463-1239 aut Enthalten in Current microbiology New York, NY : Springer, 1978 80(2023), 4 vom: 24. Feb. (DE-627)253722160 (DE-600)1458987-4 1432-0991 nnns volume:80 year:2023 number:4 day:24 month:02 https://dx.doi.org/10.1007/s00284-023-03214-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 80 2023 4 24 02 |
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Cho, Gyu-Sung @@aut@@ Mühle, Estelle @@aut@@ Clermont, Dominique @@aut@@ Franz, Charles M. A. P. @@aut@@ Besaury, Ludovic @@aut@@ |
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Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The Gram-negative, oxidase-negative, catalase-positive, rod-shaped strain $ Arafor3^{T} $ was isolated from forest soil (France). Comparative 16S rRNA gene analysis and phylogenetic analysis based on (1) multilocus sequence analysis (MLSA) with four housekeeping genes (atpD, gyrB, infB and rpoB) and (2) genomes indicated that strain $ Arafor3^{T} $ shared 98.83% 16S rRNA gene sequence similarity with the type strain of Serratia fonticola DSM $ 4576^{T} $ and was closely related to this same strain in the MLSA and in the phylogenomic tree reconstruction. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) comparisons of strain $ Arafor3^{T} $ with its nearest neighbor S. fonticola DSM $ 4576^{T} $ showed 93.5% identity and 55.7% sequence similarity, respectively, and were lower than the 96% and 70% species-level cut-off values relating to these analyses (Logan et al. in Int J Syst Evol Microbiol 59:2114–21, 2009, https://doi.org/10.1099/ijs.0.013649-0). The strain differed from S. fonticola in that it was urease and arginine dihydrolase negative. The major fatty acids of strain $ Arafor3^{T} $ are $ C_{16:0} $, $ C_{16:1} $ω7c/$ C_{16:1} $ω6c, C14:0, $ C_{14:0} $ 3-OH/16:1 isoI, and $ C_{18:1} $ω7c. The major respiratory quinone is Q8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and 6 unknown lipids. The mol G + C% content of the genomic DNA of strain $ Arafor3^{T} $ was 53.49%. 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Cho, Gyu-Sung |
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Cho, Gyu-Sung Serratia silvae sp. nov., Isolated from Forest Soil |
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Serratia silvae sp. nov., Isolated from Forest Soil |
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Serratia silvae sp. nov., Isolated from Forest Soil |
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Serratia silvae sp. nov., Isolated from Forest Soil |
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Cho, Gyu-Sung |
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Cho, Gyu-Sung Mühle, Estelle Clermont, Dominique Franz, Charles M. A. P. Besaury, Ludovic |
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serratia silvae sp. nov., isolated from forest soil |
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Serratia silvae sp. nov., Isolated from Forest Soil |
abstract |
Abstract The Gram-negative, oxidase-negative, catalase-positive, rod-shaped strain $ Arafor3^{T} $ was isolated from forest soil (France). Comparative 16S rRNA gene analysis and phylogenetic analysis based on (1) multilocus sequence analysis (MLSA) with four housekeeping genes (atpD, gyrB, infB and rpoB) and (2) genomes indicated that strain $ Arafor3^{T} $ shared 98.83% 16S rRNA gene sequence similarity with the type strain of Serratia fonticola DSM $ 4576^{T} $ and was closely related to this same strain in the MLSA and in the phylogenomic tree reconstruction. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) comparisons of strain $ Arafor3^{T} $ with its nearest neighbor S. fonticola DSM $ 4576^{T} $ showed 93.5% identity and 55.7% sequence similarity, respectively, and were lower than the 96% and 70% species-level cut-off values relating to these analyses (Logan et al. in Int J Syst Evol Microbiol 59:2114–21, 2009, https://doi.org/10.1099/ijs.0.013649-0). The strain differed from S. fonticola in that it was urease and arginine dihydrolase negative. The major fatty acids of strain $ Arafor3^{T} $ are $ C_{16:0} $, $ C_{16:1} $ω7c/$ C_{16:1} $ω6c, C14:0, $ C_{14:0} $ 3-OH/16:1 isoI, and $ C_{18:1} $ω7c. The major respiratory quinone is Q8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and 6 unknown lipids. The mol G + C% content of the genomic DNA of strain $ Arafor3^{T} $ was 53.49%. Hence, $ Arafor3^{T} $ represents a novel species within the genus Serratia, for which the name Serratia silvae sp. nov. is proposed. The type strain is $ Arafor3^{T} $ (=LMG $ 32338^{T} $ = CIP $ 111939^{T} $). © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
abstractGer |
Abstract The Gram-negative, oxidase-negative, catalase-positive, rod-shaped strain $ Arafor3^{T} $ was isolated from forest soil (France). Comparative 16S rRNA gene analysis and phylogenetic analysis based on (1) multilocus sequence analysis (MLSA) with four housekeeping genes (atpD, gyrB, infB and rpoB) and (2) genomes indicated that strain $ Arafor3^{T} $ shared 98.83% 16S rRNA gene sequence similarity with the type strain of Serratia fonticola DSM $ 4576^{T} $ and was closely related to this same strain in the MLSA and in the phylogenomic tree reconstruction. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) comparisons of strain $ Arafor3^{T} $ with its nearest neighbor S. fonticola DSM $ 4576^{T} $ showed 93.5% identity and 55.7% sequence similarity, respectively, and were lower than the 96% and 70% species-level cut-off values relating to these analyses (Logan et al. in Int J Syst Evol Microbiol 59:2114–21, 2009, https://doi.org/10.1099/ijs.0.013649-0). The strain differed from S. fonticola in that it was urease and arginine dihydrolase negative. The major fatty acids of strain $ Arafor3^{T} $ are $ C_{16:0} $, $ C_{16:1} $ω7c/$ C_{16:1} $ω6c, C14:0, $ C_{14:0} $ 3-OH/16:1 isoI, and $ C_{18:1} $ω7c. The major respiratory quinone is Q8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and 6 unknown lipids. The mol G + C% content of the genomic DNA of strain $ Arafor3^{T} $ was 53.49%. Hence, $ Arafor3^{T} $ represents a novel species within the genus Serratia, for which the name Serratia silvae sp. nov. is proposed. The type strain is $ Arafor3^{T} $ (=LMG $ 32338^{T} $ = CIP $ 111939^{T} $). © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
abstract_unstemmed |
Abstract The Gram-negative, oxidase-negative, catalase-positive, rod-shaped strain $ Arafor3^{T} $ was isolated from forest soil (France). Comparative 16S rRNA gene analysis and phylogenetic analysis based on (1) multilocus sequence analysis (MLSA) with four housekeeping genes (atpD, gyrB, infB and rpoB) and (2) genomes indicated that strain $ Arafor3^{T} $ shared 98.83% 16S rRNA gene sequence similarity with the type strain of Serratia fonticola DSM $ 4576^{T} $ and was closely related to this same strain in the MLSA and in the phylogenomic tree reconstruction. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) comparisons of strain $ Arafor3^{T} $ with its nearest neighbor S. fonticola DSM $ 4576^{T} $ showed 93.5% identity and 55.7% sequence similarity, respectively, and were lower than the 96% and 70% species-level cut-off values relating to these analyses (Logan et al. in Int J Syst Evol Microbiol 59:2114–21, 2009, https://doi.org/10.1099/ijs.0.013649-0). The strain differed from S. fonticola in that it was urease and arginine dihydrolase negative. The major fatty acids of strain $ Arafor3^{T} $ are $ C_{16:0} $, $ C_{16:1} $ω7c/$ C_{16:1} $ω6c, C14:0, $ C_{14:0} $ 3-OH/16:1 isoI, and $ C_{18:1} $ω7c. The major respiratory quinone is Q8. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and 6 unknown lipids. The mol G + C% content of the genomic DNA of strain $ Arafor3^{T} $ was 53.49%. Hence, $ Arafor3^{T} $ represents a novel species within the genus Serratia, for which the name Serratia silvae sp. nov. is proposed. The type strain is $ Arafor3^{T} $ (=LMG $ 32338^{T} $ = CIP $ 111939^{T} $). © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
collection_details |
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container_issue |
4 |
title_short |
Serratia silvae sp. nov., Isolated from Forest Soil |
url |
https://dx.doi.org/10.1007/s00284-023-03214-2 |
remote_bool |
true |
author2 |
Mühle, Estelle Clermont, Dominique Franz, Charles M. A. P. Besaury, Ludovic |
author2Str |
Mühle, Estelle Clermont, Dominique Franz, Charles M. A. P. Besaury, Ludovic |
ppnlink |
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hochschulschrift_bool |
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doi_str |
10.1007/s00284-023-03214-2 |
up_date |
2024-07-04T02:00:26.817Z |
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|
score |
7.401286 |