Thidiazuron and LED Lighting Enhance Taxifolin and Rutin Production in Rhododendron mucronulatum Turcz. Microshoot Culture
Abstract Rhododendron mucronulatum Turcz., distributed throughout the northern region of East Asia has been considered to be an alternative natural source of taxifolin (dihydroquercetin) and rutin. The present study was conducted based on a biotechnological approach to develop an environment friendl...
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| Autor*in: |
Zaytseva, Yulianna [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| Übergeordnetes Werk: |
Enthalten in: Journal of plant growth regulation - New York, NY : Springer, 1982, 42(2022), 5 vom: 12. Aug., Seite 2933-2942 |
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| Übergeordnetes Werk: |
volume:42 ; year:2022 ; number:5 ; day:12 ; month:08 ; pages:2933-2942 |
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| DOI / URN: |
10.1007/s00344-022-10757-4 |
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SPR050222767 |
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| 520 | |a Abstract Rhododendron mucronulatum Turcz., distributed throughout the northern region of East Asia has been considered to be an alternative natural source of taxifolin (dihydroquercetin) and rutin. The present study was conducted based on a biotechnological approach to develop an environment friendly and efficient system to produce taxifolin and rutin in R. mucronulatum microshoots, using different thidiazuron (TDZ) treatments (0.1; 0.5; 2.5 µM) in combination with various types of lighting including fluorescent (FL) and light-emitting diode (LED) (R/B− 80% red + 20% blue; 5LED-20% red + 20% blue + 20% green + 20% yellow + 20% white). The highest number of shoots per explant was obtained under 0.5 µM TDZ combined with 5LED in comparison with FL lighting. Among shoot clusters obtained under different lighting types and TDZ concentrations, a considerable increase in fresh and dry weight was observed in ones cultivated on medium, supplemented with 2.5 µM TDZ under FL and 0.5 µM TDZ at R/B or 5LED. The content of total chlorophylls in R. mucronulatum microshoots increased on TDZ-free medium under FL lighting, whereas, the TDZ treatment decreased chlorophylls concentration at FL and 5LED. The use of 0.1 µM TDZ at 5LED decreased the ratio of chlorophylls a + b to carotenoids and led to the highest accumulation of taxifolin and rutin, quercetin, hyperoside, and avicularin. Thus, it has been demonstrated that the application of combined action of LED and TDZ has great potential in terms of propagation efficiency, biomass accumulation, and taxifolin and rutin production in R. mucronulatum microshoots. | ||
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10.1007/s00344-022-10757-4 doi (DE-627)SPR050222767 (SPR)s00344-022-10757-4-e DE-627 ger DE-627 rakwb eng Zaytseva, Yulianna verfasserin (orcid)0000-0001-9413-4664 aut Thidiazuron and LED Lighting Enhance Taxifolin and Rutin Production in Rhododendron mucronulatum Turcz. Microshoot Culture 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Rhododendron mucronulatum Turcz., distributed throughout the northern region of East Asia has been considered to be an alternative natural source of taxifolin (dihydroquercetin) and rutin. The present study was conducted based on a biotechnological approach to develop an environment friendly and efficient system to produce taxifolin and rutin in R. mucronulatum microshoots, using different thidiazuron (TDZ) treatments (0.1; 0.5; 2.5 µM) in combination with various types of lighting including fluorescent (FL) and light-emitting diode (LED) (R/B− 80% red + 20% blue; 5LED-20% red + 20% blue + 20% green + 20% yellow + 20% white). The highest number of shoots per explant was obtained under 0.5 µM TDZ combined with 5LED in comparison with FL lighting. Among shoot clusters obtained under different lighting types and TDZ concentrations, a considerable increase in fresh and dry weight was observed in ones cultivated on medium, supplemented with 2.5 µM TDZ under FL and 0.5 µM TDZ at R/B or 5LED. The content of total chlorophylls in R. mucronulatum microshoots increased on TDZ-free medium under FL lighting, whereas, the TDZ treatment decreased chlorophylls concentration at FL and 5LED. The use of 0.1 µM TDZ at 5LED decreased the ratio of chlorophylls a + b to carotenoids and led to the highest accumulation of taxifolin and rutin, quercetin, hyperoside, and avicularin. Thus, it has been demonstrated that the application of combined action of LED and TDZ has great potential in terms of propagation efficiency, biomass accumulation, and taxifolin and rutin production in R. mucronulatum microshoots. Biomass accumulation (dpeaa)DE-He213 HPLC analysis (dpeaa)DE-He213 In vitro culture (dpeaa)DE-He213 Phenolic compounds (dpeaa)DE-He213 Photosynthetic pigments (dpeaa)DE-He213 Petruk, Anastasia aut Novikova, Tatyana aut Enthalten in Journal of plant growth regulation New York, NY : Springer, 1982 42(2022), 5 vom: 12. Aug., Seite 2933-2942 (DE-627)254630448 (DE-600)1462091-1 1435-8107 nnns volume:42 year:2022 number:5 day:12 month:08 pages:2933-2942 https://dx.doi.org/10.1007/s00344-022-10757-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 42 2022 5 12 08 2933-2942 |
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10.1007/s00344-022-10757-4 doi (DE-627)SPR050222767 (SPR)s00344-022-10757-4-e DE-627 ger DE-627 rakwb eng Zaytseva, Yulianna verfasserin (orcid)0000-0001-9413-4664 aut Thidiazuron and LED Lighting Enhance Taxifolin and Rutin Production in Rhododendron mucronulatum Turcz. Microshoot Culture 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Rhododendron mucronulatum Turcz., distributed throughout the northern region of East Asia has been considered to be an alternative natural source of taxifolin (dihydroquercetin) and rutin. The present study was conducted based on a biotechnological approach to develop an environment friendly and efficient system to produce taxifolin and rutin in R. mucronulatum microshoots, using different thidiazuron (TDZ) treatments (0.1; 0.5; 2.5 µM) in combination with various types of lighting including fluorescent (FL) and light-emitting diode (LED) (R/B− 80% red + 20% blue; 5LED-20% red + 20% blue + 20% green + 20% yellow + 20% white). The highest number of shoots per explant was obtained under 0.5 µM TDZ combined with 5LED in comparison with FL lighting. Among shoot clusters obtained under different lighting types and TDZ concentrations, a considerable increase in fresh and dry weight was observed in ones cultivated on medium, supplemented with 2.5 µM TDZ under FL and 0.5 µM TDZ at R/B or 5LED. The content of total chlorophylls in R. mucronulatum microshoots increased on TDZ-free medium under FL lighting, whereas, the TDZ treatment decreased chlorophylls concentration at FL and 5LED. The use of 0.1 µM TDZ at 5LED decreased the ratio of chlorophylls a + b to carotenoids and led to the highest accumulation of taxifolin and rutin, quercetin, hyperoside, and avicularin. Thus, it has been demonstrated that the application of combined action of LED and TDZ has great potential in terms of propagation efficiency, biomass accumulation, and taxifolin and rutin production in R. mucronulatum microshoots. Biomass accumulation (dpeaa)DE-He213 HPLC analysis (dpeaa)DE-He213 In vitro culture (dpeaa)DE-He213 Phenolic compounds (dpeaa)DE-He213 Photosynthetic pigments (dpeaa)DE-He213 Petruk, Anastasia aut Novikova, Tatyana aut Enthalten in Journal of plant growth regulation New York, NY : Springer, 1982 42(2022), 5 vom: 12. Aug., Seite 2933-2942 (DE-627)254630448 (DE-600)1462091-1 1435-8107 nnns volume:42 year:2022 number:5 day:12 month:08 pages:2933-2942 https://dx.doi.org/10.1007/s00344-022-10757-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 42 2022 5 12 08 2933-2942 |
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10.1007/s00344-022-10757-4 doi (DE-627)SPR050222767 (SPR)s00344-022-10757-4-e DE-627 ger DE-627 rakwb eng Zaytseva, Yulianna verfasserin (orcid)0000-0001-9413-4664 aut Thidiazuron and LED Lighting Enhance Taxifolin and Rutin Production in Rhododendron mucronulatum Turcz. Microshoot Culture 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Rhododendron mucronulatum Turcz., distributed throughout the northern region of East Asia has been considered to be an alternative natural source of taxifolin (dihydroquercetin) and rutin. The present study was conducted based on a biotechnological approach to develop an environment friendly and efficient system to produce taxifolin and rutin in R. mucronulatum microshoots, using different thidiazuron (TDZ) treatments (0.1; 0.5; 2.5 µM) in combination with various types of lighting including fluorescent (FL) and light-emitting diode (LED) (R/B− 80% red + 20% blue; 5LED-20% red + 20% blue + 20% green + 20% yellow + 20% white). The highest number of shoots per explant was obtained under 0.5 µM TDZ combined with 5LED in comparison with FL lighting. Among shoot clusters obtained under different lighting types and TDZ concentrations, a considerable increase in fresh and dry weight was observed in ones cultivated on medium, supplemented with 2.5 µM TDZ under FL and 0.5 µM TDZ at R/B or 5LED. The content of total chlorophylls in R. mucronulatum microshoots increased on TDZ-free medium under FL lighting, whereas, the TDZ treatment decreased chlorophylls concentration at FL and 5LED. The use of 0.1 µM TDZ at 5LED decreased the ratio of chlorophylls a + b to carotenoids and led to the highest accumulation of taxifolin and rutin, quercetin, hyperoside, and avicularin. Thus, it has been demonstrated that the application of combined action of LED and TDZ has great potential in terms of propagation efficiency, biomass accumulation, and taxifolin and rutin production in R. mucronulatum microshoots. Biomass accumulation (dpeaa)DE-He213 HPLC analysis (dpeaa)DE-He213 In vitro culture (dpeaa)DE-He213 Phenolic compounds (dpeaa)DE-He213 Photosynthetic pigments (dpeaa)DE-He213 Petruk, Anastasia aut Novikova, Tatyana aut Enthalten in Journal of plant growth regulation New York, NY : Springer, 1982 42(2022), 5 vom: 12. Aug., Seite 2933-2942 (DE-627)254630448 (DE-600)1462091-1 1435-8107 nnns volume:42 year:2022 number:5 day:12 month:08 pages:2933-2942 https://dx.doi.org/10.1007/s00344-022-10757-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 42 2022 5 12 08 2933-2942 |
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10.1007/s00344-022-10757-4 doi (DE-627)SPR050222767 (SPR)s00344-022-10757-4-e DE-627 ger DE-627 rakwb eng Zaytseva, Yulianna verfasserin (orcid)0000-0001-9413-4664 aut Thidiazuron and LED Lighting Enhance Taxifolin and Rutin Production in Rhododendron mucronulatum Turcz. Microshoot Culture 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Rhododendron mucronulatum Turcz., distributed throughout the northern region of East Asia has been considered to be an alternative natural source of taxifolin (dihydroquercetin) and rutin. The present study was conducted based on a biotechnological approach to develop an environment friendly and efficient system to produce taxifolin and rutin in R. mucronulatum microshoots, using different thidiazuron (TDZ) treatments (0.1; 0.5; 2.5 µM) in combination with various types of lighting including fluorescent (FL) and light-emitting diode (LED) (R/B− 80% red + 20% blue; 5LED-20% red + 20% blue + 20% green + 20% yellow + 20% white). The highest number of shoots per explant was obtained under 0.5 µM TDZ combined with 5LED in comparison with FL lighting. Among shoot clusters obtained under different lighting types and TDZ concentrations, a considerable increase in fresh and dry weight was observed in ones cultivated on medium, supplemented with 2.5 µM TDZ under FL and 0.5 µM TDZ at R/B or 5LED. The content of total chlorophylls in R. mucronulatum microshoots increased on TDZ-free medium under FL lighting, whereas, the TDZ treatment decreased chlorophylls concentration at FL and 5LED. The use of 0.1 µM TDZ at 5LED decreased the ratio of chlorophylls a + b to carotenoids and led to the highest accumulation of taxifolin and rutin, quercetin, hyperoside, and avicularin. Thus, it has been demonstrated that the application of combined action of LED and TDZ has great potential in terms of propagation efficiency, biomass accumulation, and taxifolin and rutin production in R. mucronulatum microshoots. Biomass accumulation (dpeaa)DE-He213 HPLC analysis (dpeaa)DE-He213 In vitro culture (dpeaa)DE-He213 Phenolic compounds (dpeaa)DE-He213 Photosynthetic pigments (dpeaa)DE-He213 Petruk, Anastasia aut Novikova, Tatyana aut Enthalten in Journal of plant growth regulation New York, NY : Springer, 1982 42(2022), 5 vom: 12. Aug., Seite 2933-2942 (DE-627)254630448 (DE-600)1462091-1 1435-8107 nnns volume:42 year:2022 number:5 day:12 month:08 pages:2933-2942 https://dx.doi.org/10.1007/s00344-022-10757-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 42 2022 5 12 08 2933-2942 |
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10.1007/s00344-022-10757-4 doi (DE-627)SPR050222767 (SPR)s00344-022-10757-4-e DE-627 ger DE-627 rakwb eng Zaytseva, Yulianna verfasserin (orcid)0000-0001-9413-4664 aut Thidiazuron and LED Lighting Enhance Taxifolin and Rutin Production in Rhododendron mucronulatum Turcz. Microshoot Culture 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Rhododendron mucronulatum Turcz., distributed throughout the northern region of East Asia has been considered to be an alternative natural source of taxifolin (dihydroquercetin) and rutin. The present study was conducted based on a biotechnological approach to develop an environment friendly and efficient system to produce taxifolin and rutin in R. mucronulatum microshoots, using different thidiazuron (TDZ) treatments (0.1; 0.5; 2.5 µM) in combination with various types of lighting including fluorescent (FL) and light-emitting diode (LED) (R/B− 80% red + 20% blue; 5LED-20% red + 20% blue + 20% green + 20% yellow + 20% white). The highest number of shoots per explant was obtained under 0.5 µM TDZ combined with 5LED in comparison with FL lighting. Among shoot clusters obtained under different lighting types and TDZ concentrations, a considerable increase in fresh and dry weight was observed in ones cultivated on medium, supplemented with 2.5 µM TDZ under FL and 0.5 µM TDZ at R/B or 5LED. The content of total chlorophylls in R. mucronulatum microshoots increased on TDZ-free medium under FL lighting, whereas, the TDZ treatment decreased chlorophylls concentration at FL and 5LED. The use of 0.1 µM TDZ at 5LED decreased the ratio of chlorophylls a + b to carotenoids and led to the highest accumulation of taxifolin and rutin, quercetin, hyperoside, and avicularin. Thus, it has been demonstrated that the application of combined action of LED and TDZ has great potential in terms of propagation efficiency, biomass accumulation, and taxifolin and rutin production in R. mucronulatum microshoots. Biomass accumulation (dpeaa)DE-He213 HPLC analysis (dpeaa)DE-He213 In vitro culture (dpeaa)DE-He213 Phenolic compounds (dpeaa)DE-He213 Photosynthetic pigments (dpeaa)DE-He213 Petruk, Anastasia aut Novikova, Tatyana aut Enthalten in Journal of plant growth regulation New York, NY : Springer, 1982 42(2022), 5 vom: 12. Aug., Seite 2933-2942 (DE-627)254630448 (DE-600)1462091-1 1435-8107 nnns volume:42 year:2022 number:5 day:12 month:08 pages:2933-2942 https://dx.doi.org/10.1007/s00344-022-10757-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 42 2022 5 12 08 2933-2942 |
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Microshoot Culture</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2022</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Rhododendron mucronulatum Turcz., distributed throughout the northern region of East Asia has been considered to be an alternative natural source of taxifolin (dihydroquercetin) and rutin. The present study was conducted based on a biotechnological approach to develop an environment friendly and efficient system to produce taxifolin and rutin in R. mucronulatum microshoots, using different thidiazuron (TDZ) treatments (0.1; 0.5; 2.5 µM) in combination with various types of lighting including fluorescent (FL) and light-emitting diode (LED) (R/B− 80% red + 20% blue; 5LED-20% red + 20% blue + 20% green + 20% yellow + 20% white). The highest number of shoots per explant was obtained under 0.5 µM TDZ combined with 5LED in comparison with FL lighting. Among shoot clusters obtained under different lighting types and TDZ concentrations, a considerable increase in fresh and dry weight was observed in ones cultivated on medium, supplemented with 2.5 µM TDZ under FL and 0.5 µM TDZ at R/B or 5LED. The content of total chlorophylls in R. mucronulatum microshoots increased on TDZ-free medium under FL lighting, whereas, the TDZ treatment decreased chlorophylls concentration at FL and 5LED. The use of 0.1 µM TDZ at 5LED decreased the ratio of chlorophylls a + b to carotenoids and led to the highest accumulation of taxifolin and rutin, quercetin, hyperoside, and avicularin. 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Zaytseva, Yulianna |
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Zaytseva, Yulianna misc Biomass accumulation misc HPLC analysis misc In vitro culture misc Phenolic compounds misc Photosynthetic pigments Thidiazuron and LED Lighting Enhance Taxifolin and Rutin Production in Rhododendron mucronulatum Turcz. Microshoot Culture |
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Thidiazuron and LED Lighting Enhance Taxifolin and Rutin Production in Rhododendron mucronulatum Turcz. Microshoot Culture Biomass accumulation (dpeaa)DE-He213 HPLC analysis (dpeaa)DE-He213 In vitro culture (dpeaa)DE-He213 Phenolic compounds (dpeaa)DE-He213 Photosynthetic pigments (dpeaa)DE-He213 |
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Thidiazuron and LED Lighting Enhance Taxifolin and Rutin Production in Rhododendron mucronulatum Turcz. Microshoot Culture |
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thidiazuron and led lighting enhance taxifolin and rutin production in rhododendron mucronulatum turcz. microshoot culture |
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Thidiazuron and LED Lighting Enhance Taxifolin and Rutin Production in Rhododendron mucronulatum Turcz. Microshoot Culture |
| abstract |
Abstract Rhododendron mucronulatum Turcz., distributed throughout the northern region of East Asia has been considered to be an alternative natural source of taxifolin (dihydroquercetin) and rutin. The present study was conducted based on a biotechnological approach to develop an environment friendly and efficient system to produce taxifolin and rutin in R. mucronulatum microshoots, using different thidiazuron (TDZ) treatments (0.1; 0.5; 2.5 µM) in combination with various types of lighting including fluorescent (FL) and light-emitting diode (LED) (R/B− 80% red + 20% blue; 5LED-20% red + 20% blue + 20% green + 20% yellow + 20% white). The highest number of shoots per explant was obtained under 0.5 µM TDZ combined with 5LED in comparison with FL lighting. Among shoot clusters obtained under different lighting types and TDZ concentrations, a considerable increase in fresh and dry weight was observed in ones cultivated on medium, supplemented with 2.5 µM TDZ under FL and 0.5 µM TDZ at R/B or 5LED. The content of total chlorophylls in R. mucronulatum microshoots increased on TDZ-free medium under FL lighting, whereas, the TDZ treatment decreased chlorophylls concentration at FL and 5LED. The use of 0.1 µM TDZ at 5LED decreased the ratio of chlorophylls a + b to carotenoids and led to the highest accumulation of taxifolin and rutin, quercetin, hyperoside, and avicularin. Thus, it has been demonstrated that the application of combined action of LED and TDZ has great potential in terms of propagation efficiency, biomass accumulation, and taxifolin and rutin production in R. mucronulatum microshoots. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
| abstractGer |
Abstract Rhododendron mucronulatum Turcz., distributed throughout the northern region of East Asia has been considered to be an alternative natural source of taxifolin (dihydroquercetin) and rutin. The present study was conducted based on a biotechnological approach to develop an environment friendly and efficient system to produce taxifolin and rutin in R. mucronulatum microshoots, using different thidiazuron (TDZ) treatments (0.1; 0.5; 2.5 µM) in combination with various types of lighting including fluorescent (FL) and light-emitting diode (LED) (R/B− 80% red + 20% blue; 5LED-20% red + 20% blue + 20% green + 20% yellow + 20% white). The highest number of shoots per explant was obtained under 0.5 µM TDZ combined with 5LED in comparison with FL lighting. Among shoot clusters obtained under different lighting types and TDZ concentrations, a considerable increase in fresh and dry weight was observed in ones cultivated on medium, supplemented with 2.5 µM TDZ under FL and 0.5 µM TDZ at R/B or 5LED. The content of total chlorophylls in R. mucronulatum microshoots increased on TDZ-free medium under FL lighting, whereas, the TDZ treatment decreased chlorophylls concentration at FL and 5LED. The use of 0.1 µM TDZ at 5LED decreased the ratio of chlorophylls a + b to carotenoids and led to the highest accumulation of taxifolin and rutin, quercetin, hyperoside, and avicularin. Thus, it has been demonstrated that the application of combined action of LED and TDZ has great potential in terms of propagation efficiency, biomass accumulation, and taxifolin and rutin production in R. mucronulatum microshoots. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
| abstract_unstemmed |
Abstract Rhododendron mucronulatum Turcz., distributed throughout the northern region of East Asia has been considered to be an alternative natural source of taxifolin (dihydroquercetin) and rutin. The present study was conducted based on a biotechnological approach to develop an environment friendly and efficient system to produce taxifolin and rutin in R. mucronulatum microshoots, using different thidiazuron (TDZ) treatments (0.1; 0.5; 2.5 µM) in combination with various types of lighting including fluorescent (FL) and light-emitting diode (LED) (R/B− 80% red + 20% blue; 5LED-20% red + 20% blue + 20% green + 20% yellow + 20% white). The highest number of shoots per explant was obtained under 0.5 µM TDZ combined with 5LED in comparison with FL lighting. Among shoot clusters obtained under different lighting types and TDZ concentrations, a considerable increase in fresh and dry weight was observed in ones cultivated on medium, supplemented with 2.5 µM TDZ under FL and 0.5 µM TDZ at R/B or 5LED. The content of total chlorophylls in R. mucronulatum microshoots increased on TDZ-free medium under FL lighting, whereas, the TDZ treatment decreased chlorophylls concentration at FL and 5LED. The use of 0.1 µM TDZ at 5LED decreased the ratio of chlorophylls a + b to carotenoids and led to the highest accumulation of taxifolin and rutin, quercetin, hyperoside, and avicularin. Thus, it has been demonstrated that the application of combined action of LED and TDZ has great potential in terms of propagation efficiency, biomass accumulation, and taxifolin and rutin production in R. mucronulatum microshoots. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| title_short |
Thidiazuron and LED Lighting Enhance Taxifolin and Rutin Production in Rhododendron mucronulatum Turcz. Microshoot Culture |
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https://dx.doi.org/10.1007/s00344-022-10757-4 |
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Petruk, Anastasia Novikova, Tatyana |
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10.1007/s00344-022-10757-4 |
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| score |
7.3999777 |