Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty

Background A variety of neurons in hypothalamus undergo a complicated regulation on transcription activity of multiple genes for hypothalamic–pituitary–gonadal axis activation during pubertal development. Identification of puberty-associated cell composition and characterization of the unique transc...
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Autor*in:	Zhou, Shasha [verfasserIn] Zang, Shaolian Hu, Yanping Shen, Yifen Li, Hua Chen, Wenlian Li, Pin Shen, Yihang

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Puberty Hypothalamus Spatial transcriptome ARC Kiss1 Slc18a3

Anmerkung:	© The Author(s) 2022

Übergeordnetes Werk:	Enthalten in: Cell & bioscience - London : BioMed Central, 2011, 12(2022), 1 vom: 21. Jan.
Übergeordnetes Werk:	volume:12 ; year:2022 ; number:1 ; day:21 ; month:01

Links:	Volltext

DOI / URN:	10.1186/s13578-022-00745-2

Katalog-ID:	SPR050432354

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520			\|a Background A variety of neurons in hypothalamus undergo a complicated regulation on transcription activity of multiple genes for hypothalamic–pituitary–gonadal axis activation during pubertal development. Identification of puberty-associated cell composition and characterization of the unique transcriptional signatures across different cells are beneficial to isolation of specific neurons and advanced understanding of their functions. Methods The hypothalamus of female Sprague–Dawley rats in postnatal day-25, 35 and 45 were used to define the dynamic spatial atlas of gene expression in the arcuate nucleus (ARC) by 10× Genomics Visium platform. A surface protein expressed selectively by kisspeptin neurons was used to sort neurons by flow cytometric assay in vitro. The transcriptome of the isolated cells was examined using Smart sequencing. Results Four subclusters of neurons with similar gene expression signatures in ARC were identified. Only one subcluster showed the robust expression of Kiss1, which could be isolated by a unique membrane surface biomarker Solute carrier family 18 member A3 (SLC18A3). Moreover, genes in different subclusters presenting three expression modules distinctly functioned in each pubertal stage. Different types of cells representing distinct functions on glial or neuron differentiation, hormone secretion as well as estradiol response precisely affect and coordinate with each other, resulting in a complicated regulatory network for hypothalamic–pituitary–gonadal axis initiation and modulation. Conclusion Our data revealed a comprehensive transcriptomic overview of ARC within different pubertal stages, which could serve as a valuable resource for the study of puberty and sexual development disorders.
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allfields	10.1186/s13578-022-00745-2 doi (DE-627)SPR050432354 (SPR)s13578-022-00745-2-e DE-627 ger DE-627 rakwb eng Zhou, Shasha verfasserin aut Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background A variety of neurons in hypothalamus undergo a complicated regulation on transcription activity of multiple genes for hypothalamic–pituitary–gonadal axis activation during pubertal development. Identification of puberty-associated cell composition and characterization of the unique transcriptional signatures across different cells are beneficial to isolation of specific neurons and advanced understanding of their functions. Methods The hypothalamus of female Sprague–Dawley rats in postnatal day-25, 35 and 45 were used to define the dynamic spatial atlas of gene expression in the arcuate nucleus (ARC) by 10× Genomics Visium platform. A surface protein expressed selectively by kisspeptin neurons was used to sort neurons by flow cytometric assay in vitro. The transcriptome of the isolated cells was examined using Smart sequencing. Results Four subclusters of neurons with similar gene expression signatures in ARC were identified. Only one subcluster showed the robust expression of Kiss1, which could be isolated by a unique membrane surface biomarker Solute carrier family 18 member A3 (SLC18A3). Moreover, genes in different subclusters presenting three expression modules distinctly functioned in each pubertal stage. Different types of cells representing distinct functions on glial or neuron differentiation, hormone secretion as well as estradiol response precisely affect and coordinate with each other, resulting in a complicated regulatory network for hypothalamic–pituitary–gonadal axis initiation and modulation. Conclusion Our data revealed a comprehensive transcriptomic overview of ARC within different pubertal stages, which could serve as a valuable resource for the study of puberty and sexual development disorders. Puberty (dpeaa)DE-He213 Hypothalamus (dpeaa)DE-He213 Spatial transcriptome (dpeaa)DE-He213 ARC (dpeaa)DE-He213 Kiss1 (dpeaa)DE-He213 Slc18a3 (dpeaa)DE-He213 Zang, Shaolian aut Hu, Yanping aut Shen, Yifen aut Li, Hua aut Chen, Wenlian aut Li, Pin aut Shen, Yihang (orcid)0000-0002-2923-1641 aut Enthalten in Cell & bioscience London : BioMed Central, 2011 12(2022), 1 vom: 21. Jan. (DE-627)646079387 (DE-600)2593367-X 2045-3701 nnns volume:12 year:2022 number:1 day:21 month:01 https://dx.doi.org/10.1186/s13578-022-00745-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 1 21 01
spelling	10.1186/s13578-022-00745-2 doi (DE-627)SPR050432354 (SPR)s13578-022-00745-2-e DE-627 ger DE-627 rakwb eng Zhou, Shasha verfasserin aut Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background A variety of neurons in hypothalamus undergo a complicated regulation on transcription activity of multiple genes for hypothalamic–pituitary–gonadal axis activation during pubertal development. Identification of puberty-associated cell composition and characterization of the unique transcriptional signatures across different cells are beneficial to isolation of specific neurons and advanced understanding of their functions. Methods The hypothalamus of female Sprague–Dawley rats in postnatal day-25, 35 and 45 were used to define the dynamic spatial atlas of gene expression in the arcuate nucleus (ARC) by 10× Genomics Visium platform. A surface protein expressed selectively by kisspeptin neurons was used to sort neurons by flow cytometric assay in vitro. The transcriptome of the isolated cells was examined using Smart sequencing. Results Four subclusters of neurons with similar gene expression signatures in ARC were identified. Only one subcluster showed the robust expression of Kiss1, which could be isolated by a unique membrane surface biomarker Solute carrier family 18 member A3 (SLC18A3). Moreover, genes in different subclusters presenting three expression modules distinctly functioned in each pubertal stage. Different types of cells representing distinct functions on glial or neuron differentiation, hormone secretion as well as estradiol response precisely affect and coordinate with each other, resulting in a complicated regulatory network for hypothalamic–pituitary–gonadal axis initiation and modulation. Conclusion Our data revealed a comprehensive transcriptomic overview of ARC within different pubertal stages, which could serve as a valuable resource for the study of puberty and sexual development disorders. Puberty (dpeaa)DE-He213 Hypothalamus (dpeaa)DE-He213 Spatial transcriptome (dpeaa)DE-He213 ARC (dpeaa)DE-He213 Kiss1 (dpeaa)DE-He213 Slc18a3 (dpeaa)DE-He213 Zang, Shaolian aut Hu, Yanping aut Shen, Yifen aut Li, Hua aut Chen, Wenlian aut Li, Pin aut Shen, Yihang (orcid)0000-0002-2923-1641 aut Enthalten in Cell & bioscience London : BioMed Central, 2011 12(2022), 1 vom: 21. Jan. (DE-627)646079387 (DE-600)2593367-X 2045-3701 nnns volume:12 year:2022 number:1 day:21 month:01 https://dx.doi.org/10.1186/s13578-022-00745-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 1 21 01
allfields_unstemmed	10.1186/s13578-022-00745-2 doi (DE-627)SPR050432354 (SPR)s13578-022-00745-2-e DE-627 ger DE-627 rakwb eng Zhou, Shasha verfasserin aut Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background A variety of neurons in hypothalamus undergo a complicated regulation on transcription activity of multiple genes for hypothalamic–pituitary–gonadal axis activation during pubertal development. Identification of puberty-associated cell composition and characterization of the unique transcriptional signatures across different cells are beneficial to isolation of specific neurons and advanced understanding of their functions. Methods The hypothalamus of female Sprague–Dawley rats in postnatal day-25, 35 and 45 were used to define the dynamic spatial atlas of gene expression in the arcuate nucleus (ARC) by 10× Genomics Visium platform. A surface protein expressed selectively by kisspeptin neurons was used to sort neurons by flow cytometric assay in vitro. The transcriptome of the isolated cells was examined using Smart sequencing. Results Four subclusters of neurons with similar gene expression signatures in ARC were identified. Only one subcluster showed the robust expression of Kiss1, which could be isolated by a unique membrane surface biomarker Solute carrier family 18 member A3 (SLC18A3). Moreover, genes in different subclusters presenting three expression modules distinctly functioned in each pubertal stage. Different types of cells representing distinct functions on glial or neuron differentiation, hormone secretion as well as estradiol response precisely affect and coordinate with each other, resulting in a complicated regulatory network for hypothalamic–pituitary–gonadal axis initiation and modulation. Conclusion Our data revealed a comprehensive transcriptomic overview of ARC within different pubertal stages, which could serve as a valuable resource for the study of puberty and sexual development disorders. Puberty (dpeaa)DE-He213 Hypothalamus (dpeaa)DE-He213 Spatial transcriptome (dpeaa)DE-He213 ARC (dpeaa)DE-He213 Kiss1 (dpeaa)DE-He213 Slc18a3 (dpeaa)DE-He213 Zang, Shaolian aut Hu, Yanping aut Shen, Yifen aut Li, Hua aut Chen, Wenlian aut Li, Pin aut Shen, Yihang (orcid)0000-0002-2923-1641 aut Enthalten in Cell & bioscience London : BioMed Central, 2011 12(2022), 1 vom: 21. Jan. (DE-627)646079387 (DE-600)2593367-X 2045-3701 nnns volume:12 year:2022 number:1 day:21 month:01 https://dx.doi.org/10.1186/s13578-022-00745-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 1 21 01
allfieldsGer	10.1186/s13578-022-00745-2 doi (DE-627)SPR050432354 (SPR)s13578-022-00745-2-e DE-627 ger DE-627 rakwb eng Zhou, Shasha verfasserin aut Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background A variety of neurons in hypothalamus undergo a complicated regulation on transcription activity of multiple genes for hypothalamic–pituitary–gonadal axis activation during pubertal development. Identification of puberty-associated cell composition and characterization of the unique transcriptional signatures across different cells are beneficial to isolation of specific neurons and advanced understanding of their functions. Methods The hypothalamus of female Sprague–Dawley rats in postnatal day-25, 35 and 45 were used to define the dynamic spatial atlas of gene expression in the arcuate nucleus (ARC) by 10× Genomics Visium platform. A surface protein expressed selectively by kisspeptin neurons was used to sort neurons by flow cytometric assay in vitro. The transcriptome of the isolated cells was examined using Smart sequencing. Results Four subclusters of neurons with similar gene expression signatures in ARC were identified. Only one subcluster showed the robust expression of Kiss1, which could be isolated by a unique membrane surface biomarker Solute carrier family 18 member A3 (SLC18A3). Moreover, genes in different subclusters presenting three expression modules distinctly functioned in each pubertal stage. Different types of cells representing distinct functions on glial or neuron differentiation, hormone secretion as well as estradiol response precisely affect and coordinate with each other, resulting in a complicated regulatory network for hypothalamic–pituitary–gonadal axis initiation and modulation. Conclusion Our data revealed a comprehensive transcriptomic overview of ARC within different pubertal stages, which could serve as a valuable resource for the study of puberty and sexual development disorders. Puberty (dpeaa)DE-He213 Hypothalamus (dpeaa)DE-He213 Spatial transcriptome (dpeaa)DE-He213 ARC (dpeaa)DE-He213 Kiss1 (dpeaa)DE-He213 Slc18a3 (dpeaa)DE-He213 Zang, Shaolian aut Hu, Yanping aut Shen, Yifen aut Li, Hua aut Chen, Wenlian aut Li, Pin aut Shen, Yihang (orcid)0000-0002-2923-1641 aut Enthalten in Cell & bioscience London : BioMed Central, 2011 12(2022), 1 vom: 21. Jan. (DE-627)646079387 (DE-600)2593367-X 2045-3701 nnns volume:12 year:2022 number:1 day:21 month:01 https://dx.doi.org/10.1186/s13578-022-00745-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 1 21 01
allfieldsSound	10.1186/s13578-022-00745-2 doi (DE-627)SPR050432354 (SPR)s13578-022-00745-2-e DE-627 ger DE-627 rakwb eng Zhou, Shasha verfasserin aut Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background A variety of neurons in hypothalamus undergo a complicated regulation on transcription activity of multiple genes for hypothalamic–pituitary–gonadal axis activation during pubertal development. Identification of puberty-associated cell composition and characterization of the unique transcriptional signatures across different cells are beneficial to isolation of specific neurons and advanced understanding of their functions. Methods The hypothalamus of female Sprague–Dawley rats in postnatal day-25, 35 and 45 were used to define the dynamic spatial atlas of gene expression in the arcuate nucleus (ARC) by 10× Genomics Visium platform. A surface protein expressed selectively by kisspeptin neurons was used to sort neurons by flow cytometric assay in vitro. The transcriptome of the isolated cells was examined using Smart sequencing. Results Four subclusters of neurons with similar gene expression signatures in ARC were identified. Only one subcluster showed the robust expression of Kiss1, which could be isolated by a unique membrane surface biomarker Solute carrier family 18 member A3 (SLC18A3). Moreover, genes in different subclusters presenting three expression modules distinctly functioned in each pubertal stage. Different types of cells representing distinct functions on glial or neuron differentiation, hormone secretion as well as estradiol response precisely affect and coordinate with each other, resulting in a complicated regulatory network for hypothalamic–pituitary–gonadal axis initiation and modulation. Conclusion Our data revealed a comprehensive transcriptomic overview of ARC within different pubertal stages, which could serve as a valuable resource for the study of puberty and sexual development disorders. Puberty (dpeaa)DE-He213 Hypothalamus (dpeaa)DE-He213 Spatial transcriptome (dpeaa)DE-He213 ARC (dpeaa)DE-He213 Kiss1 (dpeaa)DE-He213 Slc18a3 (dpeaa)DE-He213 Zang, Shaolian aut Hu, Yanping aut Shen, Yifen aut Li, Hua aut Chen, Wenlian aut Li, Pin aut Shen, Yihang (orcid)0000-0002-2923-1641 aut Enthalten in Cell & bioscience London : BioMed Central, 2011 12(2022), 1 vom: 21. Jan. (DE-627)646079387 (DE-600)2593367-X 2045-3701 nnns volume:12 year:2022 number:1 day:21 month:01 https://dx.doi.org/10.1186/s13578-022-00745-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 1 21 01
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source	Enthalten in Cell & bioscience 12(2022), 1 vom: 21. Jan. volume:12 year:2022 number:1 day:21 month:01
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topic_facet	Puberty Hypothalamus Spatial transcriptome ARC Kiss1 Slc18a3
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authorswithroles_txt_mv	Zhou, Shasha @@aut@@ Zang, Shaolian @@aut@@ Hu, Yanping @@aut@@ Shen, Yifen @@aut@@ Li, Hua @@aut@@ Chen, Wenlian @@aut@@ Li, Pin @@aut@@ Shen, Yihang @@aut@@
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Identification of puberty-associated cell composition and characterization of the unique transcriptional signatures across different cells are beneficial to isolation of specific neurons and advanced understanding of their functions. Methods The hypothalamus of female Sprague–Dawley rats in postnatal day-25, 35 and 45 were used to define the dynamic spatial atlas of gene expression in the arcuate nucleus (ARC) by 10× Genomics Visium platform. A surface protein expressed selectively by kisspeptin neurons was used to sort neurons by flow cytometric assay in vitro. The transcriptome of the isolated cells was examined using Smart sequencing. Results Four subclusters of neurons with similar gene expression signatures in ARC were identified. Only one subcluster showed the robust expression of Kiss1, which could be isolated by a unique membrane surface biomarker Solute carrier family 18 member A3 (SLC18A3). Moreover, genes in different subclusters presenting three expression modules distinctly functioned in each pubertal stage. Different types of cells representing distinct functions on glial or neuron differentiation, hormone secretion as well as estradiol response precisely affect and coordinate with each other, resulting in a complicated regulatory network for hypothalamic–pituitary–gonadal axis initiation and modulation. 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author	Zhou, Shasha
spellingShingle	Zhou, Shasha misc Puberty misc Hypothalamus misc Spatial transcriptome misc ARC misc Kiss1 misc Slc18a3 Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty
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topic_title	Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty Puberty (dpeaa)DE-He213 Hypothalamus (dpeaa)DE-He213 Spatial transcriptome (dpeaa)DE-He213 ARC (dpeaa)DE-He213 Kiss1 (dpeaa)DE-He213 Slc18a3 (dpeaa)DE-He213
topic	misc Puberty misc Hypothalamus misc Spatial transcriptome misc ARC misc Kiss1 misc Slc18a3
topic_unstemmed	misc Puberty misc Hypothalamus misc Spatial transcriptome misc ARC misc Kiss1 misc Slc18a3
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title	Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty
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title_full	Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty
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author_browse	Zhou, Shasha Zang, Shaolian Hu, Yanping Shen, Yifen Li, Hua Chen, Wenlian Li, Pin Shen, Yihang
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title_sort	transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty
title_auth	Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty
abstract	Background A variety of neurons in hypothalamus undergo a complicated regulation on transcription activity of multiple genes for hypothalamic–pituitary–gonadal axis activation during pubertal development. Identification of puberty-associated cell composition and characterization of the unique transcriptional signatures across different cells are beneficial to isolation of specific neurons and advanced understanding of their functions. Methods The hypothalamus of female Sprague–Dawley rats in postnatal day-25, 35 and 45 were used to define the dynamic spatial atlas of gene expression in the arcuate nucleus (ARC) by 10× Genomics Visium platform. A surface protein expressed selectively by kisspeptin neurons was used to sort neurons by flow cytometric assay in vitro. The transcriptome of the isolated cells was examined using Smart sequencing. Results Four subclusters of neurons with similar gene expression signatures in ARC were identified. Only one subcluster showed the robust expression of Kiss1, which could be isolated by a unique membrane surface biomarker Solute carrier family 18 member A3 (SLC18A3). Moreover, genes in different subclusters presenting three expression modules distinctly functioned in each pubertal stage. Different types of cells representing distinct functions on glial or neuron differentiation, hormone secretion as well as estradiol response precisely affect and coordinate with each other, resulting in a complicated regulatory network for hypothalamic–pituitary–gonadal axis initiation and modulation. Conclusion Our data revealed a comprehensive transcriptomic overview of ARC within different pubertal stages, which could serve as a valuable resource for the study of puberty and sexual development disorders. © The Author(s) 2022
abstractGer	Background A variety of neurons in hypothalamus undergo a complicated regulation on transcription activity of multiple genes for hypothalamic–pituitary–gonadal axis activation during pubertal development. Identification of puberty-associated cell composition and characterization of the unique transcriptional signatures across different cells are beneficial to isolation of specific neurons and advanced understanding of their functions. Methods The hypothalamus of female Sprague–Dawley rats in postnatal day-25, 35 and 45 were used to define the dynamic spatial atlas of gene expression in the arcuate nucleus (ARC) by 10× Genomics Visium platform. A surface protein expressed selectively by kisspeptin neurons was used to sort neurons by flow cytometric assay in vitro. The transcriptome of the isolated cells was examined using Smart sequencing. Results Four subclusters of neurons with similar gene expression signatures in ARC were identified. Only one subcluster showed the robust expression of Kiss1, which could be isolated by a unique membrane surface biomarker Solute carrier family 18 member A3 (SLC18A3). Moreover, genes in different subclusters presenting three expression modules distinctly functioned in each pubertal stage. Different types of cells representing distinct functions on glial or neuron differentiation, hormone secretion as well as estradiol response precisely affect and coordinate with each other, resulting in a complicated regulatory network for hypothalamic–pituitary–gonadal axis initiation and modulation. Conclusion Our data revealed a comprehensive transcriptomic overview of ARC within different pubertal stages, which could serve as a valuable resource for the study of puberty and sexual development disorders. © The Author(s) 2022
abstract_unstemmed	Background A variety of neurons in hypothalamus undergo a complicated regulation on transcription activity of multiple genes for hypothalamic–pituitary–gonadal axis activation during pubertal development. Identification of puberty-associated cell composition and characterization of the unique transcriptional signatures across different cells are beneficial to isolation of specific neurons and advanced understanding of their functions. Methods The hypothalamus of female Sprague–Dawley rats in postnatal day-25, 35 and 45 were used to define the dynamic spatial atlas of gene expression in the arcuate nucleus (ARC) by 10× Genomics Visium platform. A surface protein expressed selectively by kisspeptin neurons was used to sort neurons by flow cytometric assay in vitro. The transcriptome of the isolated cells was examined using Smart sequencing. Results Four subclusters of neurons with similar gene expression signatures in ARC were identified. Only one subcluster showed the robust expression of Kiss1, which could be isolated by a unique membrane surface biomarker Solute carrier family 18 member A3 (SLC18A3). Moreover, genes in different subclusters presenting three expression modules distinctly functioned in each pubertal stage. Different types of cells representing distinct functions on glial or neuron differentiation, hormone secretion as well as estradiol response precisely affect and coordinate with each other, resulting in a complicated regulatory network for hypothalamic–pituitary–gonadal axis initiation and modulation. Conclusion Our data revealed a comprehensive transcriptomic overview of ARC within different pubertal stages, which could serve as a valuable resource for the study of puberty and sexual development disorders. © The Author(s) 2022
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title_short	Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty
url	https://dx.doi.org/10.1186/s13578-022-00745-2
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author2	Zang, Shaolian Hu, Yanping Shen, Yifen Li, Hua Chen, Wenlian Li, Pin Shen, Yihang
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up_date	2024-07-03T15:30:29.563Z
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Moreover, genes in different subclusters presenting three expression modules distinctly functioned in each pubertal stage. Different types of cells representing distinct functions on glial or neuron differentiation, hormone secretion as well as estradiol response precisely affect and coordinate with each other, resulting in a complicated regulatory network for hypothalamic–pituitary–gonadal axis initiation and modulation. 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Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty

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