$ CD34^{+} %$ THY1^{+} $ synovial fibroblast subset in arthritic joints has high osteoblastic and chondrogenic potentials in vitro
Objective Synovial fibroblasts (SFs) in rheumatoid arthritis (RA) and osteoarthritis (OA) play biphasic roles in joint destruction and regeneration of bone/cartilage as mesenchymal stem cells (MSCs). Although MSCs contribute to joint homeostasis, such function is impaired in arthritic joints. We hav...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Noda, Seiji [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022 |
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| Anmerkung: |
© The Author(s) 2022 |
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| Übergeordnetes Werk: |
Enthalten in: Arthritis Research & Therapy - London : BioMed Central, 1999, 24(2022), 1 vom: 15. Feb. |
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| Übergeordnetes Werk: |
volume:24 ; year:2022 ; number:1 ; day:15 ; month:02 |
| Links: |
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| DOI / URN: |
10.1186/s13075-022-02736-7 |
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| Katalog-ID: |
SPR050493280 |
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| 100 | 1 | |a Noda, Seiji |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a $ CD34^{+} %$ THY1^{+} $ synovial fibroblast subset in arthritic joints has high osteoblastic and chondrogenic potentials in vitro |
| 264 | 1 | |c 2022 | |
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| 520 | |a Objective Synovial fibroblasts (SFs) in rheumatoid arthritis (RA) and osteoarthritis (OA) play biphasic roles in joint destruction and regeneration of bone/cartilage as mesenchymal stem cells (MSCs). Although MSCs contribute to joint homeostasis, such function is impaired in arthritic joints. We have identified functionally distinct three SF subsets characterized by the expression of CD34 and THY1 as follows: $ CD34^{+} %$ THY1^{+} $, $ CD34^{−} %$ THY1^{−} $, and $ CD34^{−} %$ THY1^{+} $. The objective of this study was to clarify the differentiation potentials as MSCs in each SF subset since both molecules would be associated with the MSC function. Methods SF subsets were isolated from synovial tissues of 70 patients (RA: 18, OA: 52). Expressions of surface markers associated with MSCs (THY1, CD34, CD73, CD271, CD54, CD44, and CD29) were evaluated in fleshly isolated SF subsets by flow cytometry. The differentiation potentials of osteogenesis, chondrogenesis, and adipogenesis were evaluated with histological staining and a quantitative polymerase chain reaction of differentiation marker genes. Small interfering RNA was examined to deplete THY1 in SFs. Results The expression levels of $ THY1^{+} $, $ CD73^{+} $, and $ CD271^{+} $ were highest and those of $ CD54^{+} $ and $ CD29^{+} $ were lowest in $ CD34^{+} %$ THY1^{+} $ among three subsets. Comparing three subsets, the calcified area, alkaline phosphatase (ALP)-stained area, and cartilage matrix subset were the largest in the $ CD34^{+} %$ THY1^{+} $ subset. Consistently, the expressions of differentiation markers of the osteoblasts (RUNX2, ALPL, and OCN) or chondrocytes (ACAN) were the highest in the $ CD34^{+} %$ THY1^{+} $ subset, indicating that the $ CD34^{+} %$ THY1^{+} $ subset possessed the highest osteogenic and chondrogenic potential among three subsets, while the differentiation potentials to adipocytes were comparable among the subsets regarding lipid droplet formations and the expression of LPL and PPARγ. The knockdown of THY1 in bulk SFs resulted in impaired osteoblast differentiation indicating some functional aspects in this stem-cell marker. Conclusion The $ CD34^{+} %$ THY1^{+} $ SF subset has high osteogenic and chondrogenic potentials. The preferential enhancement of MSC functions in the $ CD34^{+} %$ THY1^{+} $ subset may provide a new treatment strategy for regenerating damaged bone/cartilage in arthritic joints. | ||
| 700 | 1 | |a Hosoya, Tadashi |4 aut | |
| 700 | 1 | |a Komiya, Yoji |4 aut | |
| 700 | 1 | |a Tagawa, Yasuhiro |4 aut | |
| 700 | 1 | |a Endo, Kentaro |4 aut | |
| 700 | 1 | |a Komori, Keiichiro |4 aut | |
| 700 | 1 | |a Koga, Hideyuki |4 aut | |
| 700 | 1 | |a Takahara, Yasuhiro |4 aut | |
| 700 | 1 | |a Sugimoto, Kazutaka |4 aut | |
| 700 | 1 | |a Sekiya, Ichiro |4 aut | |
| 700 | 1 | |a Saito, Tetsuya |4 aut | |
| 700 | 1 | |a Mizoguchi, Fumitaka |4 aut | |
| 700 | 1 | |a Yasuda, Shinsuke |0 (orcid)0000-0001-6171-2077 |4 aut | |
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10.1186/s13075-022-02736-7 doi (DE-627)SPR050493280 (SPR)s13075-022-02736-7-e DE-627 ger DE-627 rakwb eng Noda, Seiji verfasserin aut $ CD34^{+} %$ THY1^{+} $ synovial fibroblast subset in arthritic joints has high osteoblastic and chondrogenic potentials in vitro 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Objective Synovial fibroblasts (SFs) in rheumatoid arthritis (RA) and osteoarthritis (OA) play biphasic roles in joint destruction and regeneration of bone/cartilage as mesenchymal stem cells (MSCs). Although MSCs contribute to joint homeostasis, such function is impaired in arthritic joints. We have identified functionally distinct three SF subsets characterized by the expression of CD34 and THY1 as follows: $ CD34^{+} %$ THY1^{+} $, $ CD34^{−} %$ THY1^{−} $, and $ CD34^{−} %$ THY1^{+} $. The objective of this study was to clarify the differentiation potentials as MSCs in each SF subset since both molecules would be associated with the MSC function. Methods SF subsets were isolated from synovial tissues of 70 patients (RA: 18, OA: 52). Expressions of surface markers associated with MSCs (THY1, CD34, CD73, CD271, CD54, CD44, and CD29) were evaluated in fleshly isolated SF subsets by flow cytometry. The differentiation potentials of osteogenesis, chondrogenesis, and adipogenesis were evaluated with histological staining and a quantitative polymerase chain reaction of differentiation marker genes. Small interfering RNA was examined to deplete THY1 in SFs. Results The expression levels of $ THY1^{+} $, $ CD73^{+} $, and $ CD271^{+} $ were highest and those of $ CD54^{+} $ and $ CD29^{+} $ were lowest in $ CD34^{+} %$ THY1^{+} $ among three subsets. Comparing three subsets, the calcified area, alkaline phosphatase (ALP)-stained area, and cartilage matrix subset were the largest in the $ CD34^{+} %$ THY1^{+} $ subset. Consistently, the expressions of differentiation markers of the osteoblasts (RUNX2, ALPL, and OCN) or chondrocytes (ACAN) were the highest in the $ CD34^{+} %$ THY1^{+} $ subset, indicating that the $ CD34^{+} %$ THY1^{+} $ subset possessed the highest osteogenic and chondrogenic potential among three subsets, while the differentiation potentials to adipocytes were comparable among the subsets regarding lipid droplet formations and the expression of LPL and PPARγ. The knockdown of THY1 in bulk SFs resulted in impaired osteoblast differentiation indicating some functional aspects in this stem-cell marker. Conclusion The $ CD34^{+} %$ THY1^{+} $ SF subset has high osteogenic and chondrogenic potentials. The preferential enhancement of MSC functions in the $ CD34^{+} %$ THY1^{+} $ subset may provide a new treatment strategy for regenerating damaged bone/cartilage in arthritic joints. Hosoya, Tadashi aut Komiya, Yoji aut Tagawa, Yasuhiro aut Endo, Kentaro aut Komori, Keiichiro aut Koga, Hideyuki aut Takahara, Yasuhiro aut Sugimoto, Kazutaka aut Sekiya, Ichiro aut Saito, Tetsuya aut Mizoguchi, Fumitaka aut Yasuda, Shinsuke (orcid)0000-0001-6171-2077 aut Enthalten in Arthritis Research & Therapy London : BioMed Central, 1999 24(2022), 1 vom: 15. Feb. (DE-627)326646418 (DE-600)2041668-4 1478-6354 nnns volume:24 year:2022 number:1 day:15 month:02 https://dx.doi.org/10.1186/s13075-022-02736-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2022 1 15 02 |
| spelling |
10.1186/s13075-022-02736-7 doi (DE-627)SPR050493280 (SPR)s13075-022-02736-7-e DE-627 ger DE-627 rakwb eng Noda, Seiji verfasserin aut $ CD34^{+} %$ THY1^{+} $ synovial fibroblast subset in arthritic joints has high osteoblastic and chondrogenic potentials in vitro 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Objective Synovial fibroblasts (SFs) in rheumatoid arthritis (RA) and osteoarthritis (OA) play biphasic roles in joint destruction and regeneration of bone/cartilage as mesenchymal stem cells (MSCs). Although MSCs contribute to joint homeostasis, such function is impaired in arthritic joints. We have identified functionally distinct three SF subsets characterized by the expression of CD34 and THY1 as follows: $ CD34^{+} %$ THY1^{+} $, $ CD34^{−} %$ THY1^{−} $, and $ CD34^{−} %$ THY1^{+} $. The objective of this study was to clarify the differentiation potentials as MSCs in each SF subset since both molecules would be associated with the MSC function. Methods SF subsets were isolated from synovial tissues of 70 patients (RA: 18, OA: 52). Expressions of surface markers associated with MSCs (THY1, CD34, CD73, CD271, CD54, CD44, and CD29) were evaluated in fleshly isolated SF subsets by flow cytometry. The differentiation potentials of osteogenesis, chondrogenesis, and adipogenesis were evaluated with histological staining and a quantitative polymerase chain reaction of differentiation marker genes. Small interfering RNA was examined to deplete THY1 in SFs. Results The expression levels of $ THY1^{+} $, $ CD73^{+} $, and $ CD271^{+} $ were highest and those of $ CD54^{+} $ and $ CD29^{+} $ were lowest in $ CD34^{+} %$ THY1^{+} $ among three subsets. Comparing three subsets, the calcified area, alkaline phosphatase (ALP)-stained area, and cartilage matrix subset were the largest in the $ CD34^{+} %$ THY1^{+} $ subset. Consistently, the expressions of differentiation markers of the osteoblasts (RUNX2, ALPL, and OCN) or chondrocytes (ACAN) were the highest in the $ CD34^{+} %$ THY1^{+} $ subset, indicating that the $ CD34^{+} %$ THY1^{+} $ subset possessed the highest osteogenic and chondrogenic potential among three subsets, while the differentiation potentials to adipocytes were comparable among the subsets regarding lipid droplet formations and the expression of LPL and PPARγ. The knockdown of THY1 in bulk SFs resulted in impaired osteoblast differentiation indicating some functional aspects in this stem-cell marker. Conclusion The $ CD34^{+} %$ THY1^{+} $ SF subset has high osteogenic and chondrogenic potentials. The preferential enhancement of MSC functions in the $ CD34^{+} %$ THY1^{+} $ subset may provide a new treatment strategy for regenerating damaged bone/cartilage in arthritic joints. Hosoya, Tadashi aut Komiya, Yoji aut Tagawa, Yasuhiro aut Endo, Kentaro aut Komori, Keiichiro aut Koga, Hideyuki aut Takahara, Yasuhiro aut Sugimoto, Kazutaka aut Sekiya, Ichiro aut Saito, Tetsuya aut Mizoguchi, Fumitaka aut Yasuda, Shinsuke (orcid)0000-0001-6171-2077 aut Enthalten in Arthritis Research & Therapy London : BioMed Central, 1999 24(2022), 1 vom: 15. Feb. (DE-627)326646418 (DE-600)2041668-4 1478-6354 nnns volume:24 year:2022 number:1 day:15 month:02 https://dx.doi.org/10.1186/s13075-022-02736-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2022 1 15 02 |
| allfields_unstemmed |
10.1186/s13075-022-02736-7 doi (DE-627)SPR050493280 (SPR)s13075-022-02736-7-e DE-627 ger DE-627 rakwb eng Noda, Seiji verfasserin aut $ CD34^{+} %$ THY1^{+} $ synovial fibroblast subset in arthritic joints has high osteoblastic and chondrogenic potentials in vitro 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Objective Synovial fibroblasts (SFs) in rheumatoid arthritis (RA) and osteoarthritis (OA) play biphasic roles in joint destruction and regeneration of bone/cartilage as mesenchymal stem cells (MSCs). Although MSCs contribute to joint homeostasis, such function is impaired in arthritic joints. We have identified functionally distinct three SF subsets characterized by the expression of CD34 and THY1 as follows: $ CD34^{+} %$ THY1^{+} $, $ CD34^{−} %$ THY1^{−} $, and $ CD34^{−} %$ THY1^{+} $. The objective of this study was to clarify the differentiation potentials as MSCs in each SF subset since both molecules would be associated with the MSC function. Methods SF subsets were isolated from synovial tissues of 70 patients (RA: 18, OA: 52). Expressions of surface markers associated with MSCs (THY1, CD34, CD73, CD271, CD54, CD44, and CD29) were evaluated in fleshly isolated SF subsets by flow cytometry. The differentiation potentials of osteogenesis, chondrogenesis, and adipogenesis were evaluated with histological staining and a quantitative polymerase chain reaction of differentiation marker genes. Small interfering RNA was examined to deplete THY1 in SFs. Results The expression levels of $ THY1^{+} $, $ CD73^{+} $, and $ CD271^{+} $ were highest and those of $ CD54^{+} $ and $ CD29^{+} $ were lowest in $ CD34^{+} %$ THY1^{+} $ among three subsets. Comparing three subsets, the calcified area, alkaline phosphatase (ALP)-stained area, and cartilage matrix subset were the largest in the $ CD34^{+} %$ THY1^{+} $ subset. Consistently, the expressions of differentiation markers of the osteoblasts (RUNX2, ALPL, and OCN) or chondrocytes (ACAN) were the highest in the $ CD34^{+} %$ THY1^{+} $ subset, indicating that the $ CD34^{+} %$ THY1^{+} $ subset possessed the highest osteogenic and chondrogenic potential among three subsets, while the differentiation potentials to adipocytes were comparable among the subsets regarding lipid droplet formations and the expression of LPL and PPARγ. The knockdown of THY1 in bulk SFs resulted in impaired osteoblast differentiation indicating some functional aspects in this stem-cell marker. Conclusion The $ CD34^{+} %$ THY1^{+} $ SF subset has high osteogenic and chondrogenic potentials. The preferential enhancement of MSC functions in the $ CD34^{+} %$ THY1^{+} $ subset may provide a new treatment strategy for regenerating damaged bone/cartilage in arthritic joints. Hosoya, Tadashi aut Komiya, Yoji aut Tagawa, Yasuhiro aut Endo, Kentaro aut Komori, Keiichiro aut Koga, Hideyuki aut Takahara, Yasuhiro aut Sugimoto, Kazutaka aut Sekiya, Ichiro aut Saito, Tetsuya aut Mizoguchi, Fumitaka aut Yasuda, Shinsuke (orcid)0000-0001-6171-2077 aut Enthalten in Arthritis Research & Therapy London : BioMed Central, 1999 24(2022), 1 vom: 15. Feb. (DE-627)326646418 (DE-600)2041668-4 1478-6354 nnns volume:24 year:2022 number:1 day:15 month:02 https://dx.doi.org/10.1186/s13075-022-02736-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2022 1 15 02 |
| allfieldsGer |
10.1186/s13075-022-02736-7 doi (DE-627)SPR050493280 (SPR)s13075-022-02736-7-e DE-627 ger DE-627 rakwb eng Noda, Seiji verfasserin aut $ CD34^{+} %$ THY1^{+} $ synovial fibroblast subset in arthritic joints has high osteoblastic and chondrogenic potentials in vitro 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Objective Synovial fibroblasts (SFs) in rheumatoid arthritis (RA) and osteoarthritis (OA) play biphasic roles in joint destruction and regeneration of bone/cartilage as mesenchymal stem cells (MSCs). Although MSCs contribute to joint homeostasis, such function is impaired in arthritic joints. We have identified functionally distinct three SF subsets characterized by the expression of CD34 and THY1 as follows: $ CD34^{+} %$ THY1^{+} $, $ CD34^{−} %$ THY1^{−} $, and $ CD34^{−} %$ THY1^{+} $. The objective of this study was to clarify the differentiation potentials as MSCs in each SF subset since both molecules would be associated with the MSC function. Methods SF subsets were isolated from synovial tissues of 70 patients (RA: 18, OA: 52). Expressions of surface markers associated with MSCs (THY1, CD34, CD73, CD271, CD54, CD44, and CD29) were evaluated in fleshly isolated SF subsets by flow cytometry. The differentiation potentials of osteogenesis, chondrogenesis, and adipogenesis were evaluated with histological staining and a quantitative polymerase chain reaction of differentiation marker genes. Small interfering RNA was examined to deplete THY1 in SFs. Results The expression levels of $ THY1^{+} $, $ CD73^{+} $, and $ CD271^{+} $ were highest and those of $ CD54^{+} $ and $ CD29^{+} $ were lowest in $ CD34^{+} %$ THY1^{+} $ among three subsets. Comparing three subsets, the calcified area, alkaline phosphatase (ALP)-stained area, and cartilage matrix subset were the largest in the $ CD34^{+} %$ THY1^{+} $ subset. Consistently, the expressions of differentiation markers of the osteoblasts (RUNX2, ALPL, and OCN) or chondrocytes (ACAN) were the highest in the $ CD34^{+} %$ THY1^{+} $ subset, indicating that the $ CD34^{+} %$ THY1^{+} $ subset possessed the highest osteogenic and chondrogenic potential among three subsets, while the differentiation potentials to adipocytes were comparable among the subsets regarding lipid droplet formations and the expression of LPL and PPARγ. The knockdown of THY1 in bulk SFs resulted in impaired osteoblast differentiation indicating some functional aspects in this stem-cell marker. Conclusion The $ CD34^{+} %$ THY1^{+} $ SF subset has high osteogenic and chondrogenic potentials. The preferential enhancement of MSC functions in the $ CD34^{+} %$ THY1^{+} $ subset may provide a new treatment strategy for regenerating damaged bone/cartilage in arthritic joints. Hosoya, Tadashi aut Komiya, Yoji aut Tagawa, Yasuhiro aut Endo, Kentaro aut Komori, Keiichiro aut Koga, Hideyuki aut Takahara, Yasuhiro aut Sugimoto, Kazutaka aut Sekiya, Ichiro aut Saito, Tetsuya aut Mizoguchi, Fumitaka aut Yasuda, Shinsuke (orcid)0000-0001-6171-2077 aut Enthalten in Arthritis Research & Therapy London : BioMed Central, 1999 24(2022), 1 vom: 15. Feb. (DE-627)326646418 (DE-600)2041668-4 1478-6354 nnns volume:24 year:2022 number:1 day:15 month:02 https://dx.doi.org/10.1186/s13075-022-02736-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2022 1 15 02 |
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10.1186/s13075-022-02736-7 doi (DE-627)SPR050493280 (SPR)s13075-022-02736-7-e DE-627 ger DE-627 rakwb eng Noda, Seiji verfasserin aut $ CD34^{+} %$ THY1^{+} $ synovial fibroblast subset in arthritic joints has high osteoblastic and chondrogenic potentials in vitro 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Objective Synovial fibroblasts (SFs) in rheumatoid arthritis (RA) and osteoarthritis (OA) play biphasic roles in joint destruction and regeneration of bone/cartilage as mesenchymal stem cells (MSCs). Although MSCs contribute to joint homeostasis, such function is impaired in arthritic joints. We have identified functionally distinct three SF subsets characterized by the expression of CD34 and THY1 as follows: $ CD34^{+} %$ THY1^{+} $, $ CD34^{−} %$ THY1^{−} $, and $ CD34^{−} %$ THY1^{+} $. The objective of this study was to clarify the differentiation potentials as MSCs in each SF subset since both molecules would be associated with the MSC function. Methods SF subsets were isolated from synovial tissues of 70 patients (RA: 18, OA: 52). Expressions of surface markers associated with MSCs (THY1, CD34, CD73, CD271, CD54, CD44, and CD29) were evaluated in fleshly isolated SF subsets by flow cytometry. The differentiation potentials of osteogenesis, chondrogenesis, and adipogenesis were evaluated with histological staining and a quantitative polymerase chain reaction of differentiation marker genes. Small interfering RNA was examined to deplete THY1 in SFs. Results The expression levels of $ THY1^{+} $, $ CD73^{+} $, and $ CD271^{+} $ were highest and those of $ CD54^{+} $ and $ CD29^{+} $ were lowest in $ CD34^{+} %$ THY1^{+} $ among three subsets. Comparing three subsets, the calcified area, alkaline phosphatase (ALP)-stained area, and cartilage matrix subset were the largest in the $ CD34^{+} %$ THY1^{+} $ subset. Consistently, the expressions of differentiation markers of the osteoblasts (RUNX2, ALPL, and OCN) or chondrocytes (ACAN) were the highest in the $ CD34^{+} %$ THY1^{+} $ subset, indicating that the $ CD34^{+} %$ THY1^{+} $ subset possessed the highest osteogenic and chondrogenic potential among three subsets, while the differentiation potentials to adipocytes were comparable among the subsets regarding lipid droplet formations and the expression of LPL and PPARγ. The knockdown of THY1 in bulk SFs resulted in impaired osteoblast differentiation indicating some functional aspects in this stem-cell marker. Conclusion The $ CD34^{+} %$ THY1^{+} $ SF subset has high osteogenic and chondrogenic potentials. The preferential enhancement of MSC functions in the $ CD34^{+} %$ THY1^{+} $ subset may provide a new treatment strategy for regenerating damaged bone/cartilage in arthritic joints. Hosoya, Tadashi aut Komiya, Yoji aut Tagawa, Yasuhiro aut Endo, Kentaro aut Komori, Keiichiro aut Koga, Hideyuki aut Takahara, Yasuhiro aut Sugimoto, Kazutaka aut Sekiya, Ichiro aut Saito, Tetsuya aut Mizoguchi, Fumitaka aut Yasuda, Shinsuke (orcid)0000-0001-6171-2077 aut Enthalten in Arthritis Research & Therapy London : BioMed Central, 1999 24(2022), 1 vom: 15. Feb. (DE-627)326646418 (DE-600)2041668-4 1478-6354 nnns volume:24 year:2022 number:1 day:15 month:02 https://dx.doi.org/10.1186/s13075-022-02736-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2022 1 15 02 |
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Noda, Seiji Hosoya, Tadashi Komiya, Yoji Tagawa, Yasuhiro Endo, Kentaro Komori, Keiichiro Koga, Hideyuki Takahara, Yasuhiro Sugimoto, Kazutaka Sekiya, Ichiro Saito, Tetsuya Mizoguchi, Fumitaka Yasuda, Shinsuke |
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$ cd34^{+} %$ thy1^{+} $ synovial fibroblast subset in arthritic joints has high osteoblastic and chondrogenic potentials in vitro |
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$ CD34^{+} %$ THY1^{+} $ synovial fibroblast subset in arthritic joints has high osteoblastic and chondrogenic potentials in vitro |
| abstract |
Objective Synovial fibroblasts (SFs) in rheumatoid arthritis (RA) and osteoarthritis (OA) play biphasic roles in joint destruction and regeneration of bone/cartilage as mesenchymal stem cells (MSCs). Although MSCs contribute to joint homeostasis, such function is impaired in arthritic joints. We have identified functionally distinct three SF subsets characterized by the expression of CD34 and THY1 as follows: $ CD34^{+} %$ THY1^{+} $, $ CD34^{−} %$ THY1^{−} $, and $ CD34^{−} %$ THY1^{+} $. The objective of this study was to clarify the differentiation potentials as MSCs in each SF subset since both molecules would be associated with the MSC function. Methods SF subsets were isolated from synovial tissues of 70 patients (RA: 18, OA: 52). Expressions of surface markers associated with MSCs (THY1, CD34, CD73, CD271, CD54, CD44, and CD29) were evaluated in fleshly isolated SF subsets by flow cytometry. The differentiation potentials of osteogenesis, chondrogenesis, and adipogenesis were evaluated with histological staining and a quantitative polymerase chain reaction of differentiation marker genes. Small interfering RNA was examined to deplete THY1 in SFs. Results The expression levels of $ THY1^{+} $, $ CD73^{+} $, and $ CD271^{+} $ were highest and those of $ CD54^{+} $ and $ CD29^{+} $ were lowest in $ CD34^{+} %$ THY1^{+} $ among three subsets. Comparing three subsets, the calcified area, alkaline phosphatase (ALP)-stained area, and cartilage matrix subset were the largest in the $ CD34^{+} %$ THY1^{+} $ subset. Consistently, the expressions of differentiation markers of the osteoblasts (RUNX2, ALPL, and OCN) or chondrocytes (ACAN) were the highest in the $ CD34^{+} %$ THY1^{+} $ subset, indicating that the $ CD34^{+} %$ THY1^{+} $ subset possessed the highest osteogenic and chondrogenic potential among three subsets, while the differentiation potentials to adipocytes were comparable among the subsets regarding lipid droplet formations and the expression of LPL and PPARγ. The knockdown of THY1 in bulk SFs resulted in impaired osteoblast differentiation indicating some functional aspects in this stem-cell marker. Conclusion The $ CD34^{+} %$ THY1^{+} $ SF subset has high osteogenic and chondrogenic potentials. The preferential enhancement of MSC functions in the $ CD34^{+} %$ THY1^{+} $ subset may provide a new treatment strategy for regenerating damaged bone/cartilage in arthritic joints. © The Author(s) 2022 |
| abstractGer |
Objective Synovial fibroblasts (SFs) in rheumatoid arthritis (RA) and osteoarthritis (OA) play biphasic roles in joint destruction and regeneration of bone/cartilage as mesenchymal stem cells (MSCs). Although MSCs contribute to joint homeostasis, such function is impaired in arthritic joints. We have identified functionally distinct three SF subsets characterized by the expression of CD34 and THY1 as follows: $ CD34^{+} %$ THY1^{+} $, $ CD34^{−} %$ THY1^{−} $, and $ CD34^{−} %$ THY1^{+} $. The objective of this study was to clarify the differentiation potentials as MSCs in each SF subset since both molecules would be associated with the MSC function. Methods SF subsets were isolated from synovial tissues of 70 patients (RA: 18, OA: 52). Expressions of surface markers associated with MSCs (THY1, CD34, CD73, CD271, CD54, CD44, and CD29) were evaluated in fleshly isolated SF subsets by flow cytometry. The differentiation potentials of osteogenesis, chondrogenesis, and adipogenesis were evaluated with histological staining and a quantitative polymerase chain reaction of differentiation marker genes. Small interfering RNA was examined to deplete THY1 in SFs. Results The expression levels of $ THY1^{+} $, $ CD73^{+} $, and $ CD271^{+} $ were highest and those of $ CD54^{+} $ and $ CD29^{+} $ were lowest in $ CD34^{+} %$ THY1^{+} $ among three subsets. Comparing three subsets, the calcified area, alkaline phosphatase (ALP)-stained area, and cartilage matrix subset were the largest in the $ CD34^{+} %$ THY1^{+} $ subset. Consistently, the expressions of differentiation markers of the osteoblasts (RUNX2, ALPL, and OCN) or chondrocytes (ACAN) were the highest in the $ CD34^{+} %$ THY1^{+} $ subset, indicating that the $ CD34^{+} %$ THY1^{+} $ subset possessed the highest osteogenic and chondrogenic potential among three subsets, while the differentiation potentials to adipocytes were comparable among the subsets regarding lipid droplet formations and the expression of LPL and PPARγ. The knockdown of THY1 in bulk SFs resulted in impaired osteoblast differentiation indicating some functional aspects in this stem-cell marker. Conclusion The $ CD34^{+} %$ THY1^{+} $ SF subset has high osteogenic and chondrogenic potentials. The preferential enhancement of MSC functions in the $ CD34^{+} %$ THY1^{+} $ subset may provide a new treatment strategy for regenerating damaged bone/cartilage in arthritic joints. © The Author(s) 2022 |
| abstract_unstemmed |
Objective Synovial fibroblasts (SFs) in rheumatoid arthritis (RA) and osteoarthritis (OA) play biphasic roles in joint destruction and regeneration of bone/cartilage as mesenchymal stem cells (MSCs). Although MSCs contribute to joint homeostasis, such function is impaired in arthritic joints. We have identified functionally distinct three SF subsets characterized by the expression of CD34 and THY1 as follows: $ CD34^{+} %$ THY1^{+} $, $ CD34^{−} %$ THY1^{−} $, and $ CD34^{−} %$ THY1^{+} $. The objective of this study was to clarify the differentiation potentials as MSCs in each SF subset since both molecules would be associated with the MSC function. Methods SF subsets were isolated from synovial tissues of 70 patients (RA: 18, OA: 52). Expressions of surface markers associated with MSCs (THY1, CD34, CD73, CD271, CD54, CD44, and CD29) were evaluated in fleshly isolated SF subsets by flow cytometry. The differentiation potentials of osteogenesis, chondrogenesis, and adipogenesis were evaluated with histological staining and a quantitative polymerase chain reaction of differentiation marker genes. Small interfering RNA was examined to deplete THY1 in SFs. Results The expression levels of $ THY1^{+} $, $ CD73^{+} $, and $ CD271^{+} $ were highest and those of $ CD54^{+} $ and $ CD29^{+} $ were lowest in $ CD34^{+} %$ THY1^{+} $ among three subsets. Comparing three subsets, the calcified area, alkaline phosphatase (ALP)-stained area, and cartilage matrix subset were the largest in the $ CD34^{+} %$ THY1^{+} $ subset. Consistently, the expressions of differentiation markers of the osteoblasts (RUNX2, ALPL, and OCN) or chondrocytes (ACAN) were the highest in the $ CD34^{+} %$ THY1^{+} $ subset, indicating that the $ CD34^{+} %$ THY1^{+} $ subset possessed the highest osteogenic and chondrogenic potential among three subsets, while the differentiation potentials to adipocytes were comparable among the subsets regarding lipid droplet formations and the expression of LPL and PPARγ. The knockdown of THY1 in bulk SFs resulted in impaired osteoblast differentiation indicating some functional aspects in this stem-cell marker. Conclusion The $ CD34^{+} %$ THY1^{+} $ SF subset has high osteogenic and chondrogenic potentials. The preferential enhancement of MSC functions in the $ CD34^{+} %$ THY1^{+} $ subset may provide a new treatment strategy for regenerating damaged bone/cartilage in arthritic joints. © The Author(s) 2022 |
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| title_short |
$ CD34^{+} %$ THY1^{+} $ synovial fibroblast subset in arthritic joints has high osteoblastic and chondrogenic potentials in vitro |
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Hosoya, Tadashi Komiya, Yoji Tagawa, Yasuhiro Endo, Kentaro Komori, Keiichiro Koga, Hideyuki Takahara, Yasuhiro Sugimoto, Kazutaka Sekiya, Ichiro Saito, Tetsuya Mizoguchi, Fumitaka Yasuda, Shinsuke |
| author2Str |
Hosoya, Tadashi Komiya, Yoji Tagawa, Yasuhiro Endo, Kentaro Komori, Keiichiro Koga, Hideyuki Takahara, Yasuhiro Sugimoto, Kazutaka Sekiya, Ichiro Saito, Tetsuya Mizoguchi, Fumitaka Yasuda, Shinsuke |
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| doi_str |
10.1186/s13075-022-02736-7 |
| up_date |
2024-07-03T15:53:42.355Z |
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