Combined immunization with inactivated vaccine reduces the dose of live B. abortus A19 vaccine

Background Brucella spp. is an important zoonotic pathogen responsible for brucellosis in humans and animals. Brucella abortus A19 strain is a widespread vaccine in China. However, it has a drawback of residual virulence in animals and humans. Methods In this study, the BALB/c mice were inoculated w...
Ausführliche Beschreibung

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Autor*in:	He, Chuan-Yu [verfasserIn] Zhang, Yu-Zhuo Liu, Meng-Zhi Zhao, Hai-Long Ren, Li-Song Liu, Bao-Shan He, Sun Chen, Ze-Liang

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Brucellosis Combined immunization Live vaccine Inactivated vaccine

Anmerkung:	© The Author(s) 2022

Übergeordnetes Werk:	Enthalten in: BMC veterinary research - London : BioMed Central, 2005, 18(2022), 1 vom: 02. Apr.
Übergeordnetes Werk:	volume:18 ; year:2022 ; number:1 ; day:02 ; month:04

Links:	Volltext

DOI / URN:	10.1186/s12917-022-03229-0

Katalog-ID:	SPR050614088

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245	1	0	\|a Combined immunization with inactivated vaccine reduces the dose of live B. abortus A19 vaccine
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520			\|a Background Brucella spp. is an important zoonotic pathogen responsible for brucellosis in humans and animals. Brucella abortus A19 strain is a widespread vaccine in China. However, it has a drawback of residual virulence in animals and humans. Methods In this study, the BALB/c mice were inoculated with either 100 μL PBS(control group, C group), $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (I group), $ 10^{5} $ CFU/mL (low-dose group, L group) $ 10^{6} $ CFU/mL live B. abortus A19 strain (high-dose group, H group), or $ 10^{5} $ CFU/mL live B. abortus A19 strain combined with $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (LI group). Mice were challenged with B. abortus strain 2308 at 7 week post vaccination. Subsequently, the immune and protective efficacy of the vaccines were evaluated by measuring splenic bacterial burden, spleen weight, serum IgG, interferon-gamma (IFN-γ), interleukin-4 (IL-4) percentage of CD4 + and CD8 + T cells of mice via bacterial isolation, weighing, ELISA and flow cytometry, respectively. Results The splenic bacterial burden and spleen weight of the mice in group LI were mostly equivalent to the mice of group H. Moreover, Brucella-specific serum IgG, IFN-γ, IL-4, and the percentage of $ CD4^{+} $ and $ CD8^{+} $ T cells of the LI group mice were similar to those of the H group. In the subsequent challenge test, both vaccines conferred protective immunity to wild-type (WT) 2308 strain. In addition, the levels of IL-4 and IFN-γ, $ CD4^{+} $ and $ CD8^{+} $ T cells in these mice were similar to those of the mice in the H group. Conclusions Combined immunization with low dose live vaccine and inactivated vaccine allowed to reduce the live B. abortus A19 vaccine, dose with an equivalent protection of the high-dose live vaccine.
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650		4	\|a Combined immunization \|7 (dpeaa)DE-He213
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650		4	\|a Inactivated vaccine \|7 (dpeaa)DE-He213
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700	1		\|a Zhao, Hai-Long \|4 aut
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700	1		\|a Liu, Bao-Shan \|4 aut
700	1		\|a He, Sun \|4 aut
700	1		\|a Chen, Ze-Liang \|4 aut
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912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
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912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
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912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
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allfields	10.1186/s12917-022-03229-0 doi (DE-627)SPR050614088 (SPR)s12917-022-03229-0-e DE-627 ger DE-627 rakwb eng He, Chuan-Yu verfasserin aut Combined immunization with inactivated vaccine reduces the dose of live B. abortus A19 vaccine 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Brucella spp. is an important zoonotic pathogen responsible for brucellosis in humans and animals. Brucella abortus A19 strain is a widespread vaccine in China. However, it has a drawback of residual virulence in animals and humans. Methods In this study, the BALB/c mice were inoculated with either 100 μL PBS(control group, C group), $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (I group), $ 10^{5} $ CFU/mL (low-dose group, L group) $ 10^{6} $ CFU/mL live B. abortus A19 strain (high-dose group, H group), or $ 10^{5} $ CFU/mL live B. abortus A19 strain combined with $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (LI group). Mice were challenged with B. abortus strain 2308 at 7 week post vaccination. Subsequently, the immune and protective efficacy of the vaccines were evaluated by measuring splenic bacterial burden, spleen weight, serum IgG, interferon-gamma (IFN-γ), interleukin-4 (IL-4) percentage of CD4 + and CD8 + T cells of mice via bacterial isolation, weighing, ELISA and flow cytometry, respectively. Results The splenic bacterial burden and spleen weight of the mice in group LI were mostly equivalent to the mice of group H. Moreover, Brucella-specific serum IgG, IFN-γ, IL-4, and the percentage of $ CD4^{+} $ and $ CD8^{+} $ T cells of the LI group mice were similar to those of the H group. In the subsequent challenge test, both vaccines conferred protective immunity to wild-type (WT) 2308 strain. In addition, the levels of IL-4 and IFN-γ, $ CD4^{+} $ and $ CD8^{+} $ T cells in these mice were similar to those of the mice in the H group. Conclusions Combined immunization with low dose live vaccine and inactivated vaccine allowed to reduce the live B. abortus A19 vaccine, dose with an equivalent protection of the high-dose live vaccine. Brucellosis (dpeaa)DE-He213 Combined immunization (dpeaa)DE-He213 Live vaccine (dpeaa)DE-He213 Inactivated vaccine (dpeaa)DE-He213 Zhang, Yu-Zhuo aut Liu, Meng-Zhi aut Zhao, Hai-Long aut Ren, Li-Song aut Liu, Bao-Shan aut He, Sun aut Chen, Ze-Liang aut Enthalten in BMC veterinary research London : BioMed Central, 2005 18(2022), 1 vom: 02. Apr. (DE-627)489256538 (DE-600)2191675-5 1746-6148 nnns volume:18 year:2022 number:1 day:02 month:04 https://dx.doi.org/10.1186/s12917-022-03229-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 18 2022 1 02 04
spelling	10.1186/s12917-022-03229-0 doi (DE-627)SPR050614088 (SPR)s12917-022-03229-0-e DE-627 ger DE-627 rakwb eng He, Chuan-Yu verfasserin aut Combined immunization with inactivated vaccine reduces the dose of live B. abortus A19 vaccine 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Brucella spp. is an important zoonotic pathogen responsible for brucellosis in humans and animals. Brucella abortus A19 strain is a widespread vaccine in China. However, it has a drawback of residual virulence in animals and humans. Methods In this study, the BALB/c mice were inoculated with either 100 μL PBS(control group, C group), $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (I group), $ 10^{5} $ CFU/mL (low-dose group, L group) $ 10^{6} $ CFU/mL live B. abortus A19 strain (high-dose group, H group), or $ 10^{5} $ CFU/mL live B. abortus A19 strain combined with $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (LI group). Mice were challenged with B. abortus strain 2308 at 7 week post vaccination. Subsequently, the immune and protective efficacy of the vaccines were evaluated by measuring splenic bacterial burden, spleen weight, serum IgG, interferon-gamma (IFN-γ), interleukin-4 (IL-4) percentage of CD4 + and CD8 + T cells of mice via bacterial isolation, weighing, ELISA and flow cytometry, respectively. Results The splenic bacterial burden and spleen weight of the mice in group LI were mostly equivalent to the mice of group H. Moreover, Brucella-specific serum IgG, IFN-γ, IL-4, and the percentage of $ CD4^{+} $ and $ CD8^{+} $ T cells of the LI group mice were similar to those of the H group. In the subsequent challenge test, both vaccines conferred protective immunity to wild-type (WT) 2308 strain. In addition, the levels of IL-4 and IFN-γ, $ CD4^{+} $ and $ CD8^{+} $ T cells in these mice were similar to those of the mice in the H group. Conclusions Combined immunization with low dose live vaccine and inactivated vaccine allowed to reduce the live B. abortus A19 vaccine, dose with an equivalent protection of the high-dose live vaccine. Brucellosis (dpeaa)DE-He213 Combined immunization (dpeaa)DE-He213 Live vaccine (dpeaa)DE-He213 Inactivated vaccine (dpeaa)DE-He213 Zhang, Yu-Zhuo aut Liu, Meng-Zhi aut Zhao, Hai-Long aut Ren, Li-Song aut Liu, Bao-Shan aut He, Sun aut Chen, Ze-Liang aut Enthalten in BMC veterinary research London : BioMed Central, 2005 18(2022), 1 vom: 02. Apr. (DE-627)489256538 (DE-600)2191675-5 1746-6148 nnns volume:18 year:2022 number:1 day:02 month:04 https://dx.doi.org/10.1186/s12917-022-03229-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 18 2022 1 02 04
allfields_unstemmed	10.1186/s12917-022-03229-0 doi (DE-627)SPR050614088 (SPR)s12917-022-03229-0-e DE-627 ger DE-627 rakwb eng He, Chuan-Yu verfasserin aut Combined immunization with inactivated vaccine reduces the dose of live B. abortus A19 vaccine 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Brucella spp. is an important zoonotic pathogen responsible for brucellosis in humans and animals. Brucella abortus A19 strain is a widespread vaccine in China. However, it has a drawback of residual virulence in animals and humans. Methods In this study, the BALB/c mice were inoculated with either 100 μL PBS(control group, C group), $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (I group), $ 10^{5} $ CFU/mL (low-dose group, L group) $ 10^{6} $ CFU/mL live B. abortus A19 strain (high-dose group, H group), or $ 10^{5} $ CFU/mL live B. abortus A19 strain combined with $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (LI group). Mice were challenged with B. abortus strain 2308 at 7 week post vaccination. Subsequently, the immune and protective efficacy of the vaccines were evaluated by measuring splenic bacterial burden, spleen weight, serum IgG, interferon-gamma (IFN-γ), interleukin-4 (IL-4) percentage of CD4 + and CD8 + T cells of mice via bacterial isolation, weighing, ELISA and flow cytometry, respectively. Results The splenic bacterial burden and spleen weight of the mice in group LI were mostly equivalent to the mice of group H. Moreover, Brucella-specific serum IgG, IFN-γ, IL-4, and the percentage of $ CD4^{+} $ and $ CD8^{+} $ T cells of the LI group mice were similar to those of the H group. In the subsequent challenge test, both vaccines conferred protective immunity to wild-type (WT) 2308 strain. In addition, the levels of IL-4 and IFN-γ, $ CD4^{+} $ and $ CD8^{+} $ T cells in these mice were similar to those of the mice in the H group. Conclusions Combined immunization with low dose live vaccine and inactivated vaccine allowed to reduce the live B. abortus A19 vaccine, dose with an equivalent protection of the high-dose live vaccine. Brucellosis (dpeaa)DE-He213 Combined immunization (dpeaa)DE-He213 Live vaccine (dpeaa)DE-He213 Inactivated vaccine (dpeaa)DE-He213 Zhang, Yu-Zhuo aut Liu, Meng-Zhi aut Zhao, Hai-Long aut Ren, Li-Song aut Liu, Bao-Shan aut He, Sun aut Chen, Ze-Liang aut Enthalten in BMC veterinary research London : BioMed Central, 2005 18(2022), 1 vom: 02. Apr. (DE-627)489256538 (DE-600)2191675-5 1746-6148 nnns volume:18 year:2022 number:1 day:02 month:04 https://dx.doi.org/10.1186/s12917-022-03229-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 18 2022 1 02 04
allfieldsGer	10.1186/s12917-022-03229-0 doi (DE-627)SPR050614088 (SPR)s12917-022-03229-0-e DE-627 ger DE-627 rakwb eng He, Chuan-Yu verfasserin aut Combined immunization with inactivated vaccine reduces the dose of live B. abortus A19 vaccine 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Brucella spp. is an important zoonotic pathogen responsible for brucellosis in humans and animals. Brucella abortus A19 strain is a widespread vaccine in China. However, it has a drawback of residual virulence in animals and humans. Methods In this study, the BALB/c mice were inoculated with either 100 μL PBS(control group, C group), $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (I group), $ 10^{5} $ CFU/mL (low-dose group, L group) $ 10^{6} $ CFU/mL live B. abortus A19 strain (high-dose group, H group), or $ 10^{5} $ CFU/mL live B. abortus A19 strain combined with $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (LI group). Mice were challenged with B. abortus strain 2308 at 7 week post vaccination. Subsequently, the immune and protective efficacy of the vaccines were evaluated by measuring splenic bacterial burden, spleen weight, serum IgG, interferon-gamma (IFN-γ), interleukin-4 (IL-4) percentage of CD4 + and CD8 + T cells of mice via bacterial isolation, weighing, ELISA and flow cytometry, respectively. Results The splenic bacterial burden and spleen weight of the mice in group LI were mostly equivalent to the mice of group H. Moreover, Brucella-specific serum IgG, IFN-γ, IL-4, and the percentage of $ CD4^{+} $ and $ CD8^{+} $ T cells of the LI group mice were similar to those of the H group. In the subsequent challenge test, both vaccines conferred protective immunity to wild-type (WT) 2308 strain. In addition, the levels of IL-4 and IFN-γ, $ CD4^{+} $ and $ CD8^{+} $ T cells in these mice were similar to those of the mice in the H group. Conclusions Combined immunization with low dose live vaccine and inactivated vaccine allowed to reduce the live B. abortus A19 vaccine, dose with an equivalent protection of the high-dose live vaccine. Brucellosis (dpeaa)DE-He213 Combined immunization (dpeaa)DE-He213 Live vaccine (dpeaa)DE-He213 Inactivated vaccine (dpeaa)DE-He213 Zhang, Yu-Zhuo aut Liu, Meng-Zhi aut Zhao, Hai-Long aut Ren, Li-Song aut Liu, Bao-Shan aut He, Sun aut Chen, Ze-Liang aut Enthalten in BMC veterinary research London : BioMed Central, 2005 18(2022), 1 vom: 02. Apr. (DE-627)489256538 (DE-600)2191675-5 1746-6148 nnns volume:18 year:2022 number:1 day:02 month:04 https://dx.doi.org/10.1186/s12917-022-03229-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 18 2022 1 02 04
allfieldsSound	10.1186/s12917-022-03229-0 doi (DE-627)SPR050614088 (SPR)s12917-022-03229-0-e DE-627 ger DE-627 rakwb eng He, Chuan-Yu verfasserin aut Combined immunization with inactivated vaccine reduces the dose of live B. abortus A19 vaccine 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Brucella spp. is an important zoonotic pathogen responsible for brucellosis in humans and animals. Brucella abortus A19 strain is a widespread vaccine in China. However, it has a drawback of residual virulence in animals and humans. Methods In this study, the BALB/c mice were inoculated with either 100 μL PBS(control group, C group), $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (I group), $ 10^{5} $ CFU/mL (low-dose group, L group) $ 10^{6} $ CFU/mL live B. abortus A19 strain (high-dose group, H group), or $ 10^{5} $ CFU/mL live B. abortus A19 strain combined with $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (LI group). Mice were challenged with B. abortus strain 2308 at 7 week post vaccination. Subsequently, the immune and protective efficacy of the vaccines were evaluated by measuring splenic bacterial burden, spleen weight, serum IgG, interferon-gamma (IFN-γ), interleukin-4 (IL-4) percentage of CD4 + and CD8 + T cells of mice via bacterial isolation, weighing, ELISA and flow cytometry, respectively. Results The splenic bacterial burden and spleen weight of the mice in group LI were mostly equivalent to the mice of group H. Moreover, Brucella-specific serum IgG, IFN-γ, IL-4, and the percentage of $ CD4^{+} $ and $ CD8^{+} $ T cells of the LI group mice were similar to those of the H group. In the subsequent challenge test, both vaccines conferred protective immunity to wild-type (WT) 2308 strain. In addition, the levels of IL-4 and IFN-γ, $ CD4^{+} $ and $ CD8^{+} $ T cells in these mice were similar to those of the mice in the H group. Conclusions Combined immunization with low dose live vaccine and inactivated vaccine allowed to reduce the live B. abortus A19 vaccine, dose with an equivalent protection of the high-dose live vaccine. Brucellosis (dpeaa)DE-He213 Combined immunization (dpeaa)DE-He213 Live vaccine (dpeaa)DE-He213 Inactivated vaccine (dpeaa)DE-He213 Zhang, Yu-Zhuo aut Liu, Meng-Zhi aut Zhao, Hai-Long aut Ren, Li-Song aut Liu, Bao-Shan aut He, Sun aut Chen, Ze-Liang aut Enthalten in BMC veterinary research London : BioMed Central, 2005 18(2022), 1 vom: 02. Apr. (DE-627)489256538 (DE-600)2191675-5 1746-6148 nnns volume:18 year:2022 number:1 day:02 month:04 https://dx.doi.org/10.1186/s12917-022-03229-0 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 18 2022 1 02 04
language	English
source	Enthalten in BMC veterinary research 18(2022), 1 vom: 02. Apr. volume:18 year:2022 number:1 day:02 month:04
sourceStr	Enthalten in BMC veterinary research 18(2022), 1 vom: 02. Apr. volume:18 year:2022 number:1 day:02 month:04
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Brucellosis Combined immunization Live vaccine Inactivated vaccine
isfreeaccess_bool	true
container_title	BMC veterinary research
authorswithroles_txt_mv	He, Chuan-Yu @@aut@@ Zhang, Yu-Zhuo @@aut@@ Liu, Meng-Zhi @@aut@@ Zhao, Hai-Long @@aut@@ Ren, Li-Song @@aut@@ Liu, Bao-Shan @@aut@@ He, Sun @@aut@@ Chen, Ze-Liang @@aut@@
publishDateDaySort_date	2022-04-02T00:00:00Z
hierarchy_top_id	489256538
id	SPR050614088
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">SPR050614088</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230507144954.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230507s2022 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1186/s12917-022-03229-0</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR050614088</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s12917-022-03229-0-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">He, Chuan-Yu</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Combined immunization with inactivated vaccine reduces the dose of live B. abortus A19 vaccine</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2022</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© The Author(s) 2022</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Background Brucella spp. is an important zoonotic pathogen responsible for brucellosis in humans and animals. Brucella abortus A19 strain is a widespread vaccine in China. However, it has a drawback of residual virulence in animals and humans. Methods In this study, the BALB/c mice were inoculated with either 100 μL PBS(control group, C group), $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (I group), $ 10^{5} $ CFU/mL (low-dose group, L group) $ 10^{6} $ CFU/mL live B. abortus A19 strain (high-dose group, H group), or $ 10^{5} $ CFU/mL live B. abortus A19 strain combined with $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (LI group). Mice were challenged with B. abortus strain 2308 at 7 week post vaccination. Subsequently, the immune and protective efficacy of the vaccines were evaluated by measuring splenic bacterial burden, spleen weight, serum IgG, interferon-gamma (IFN-γ), interleukin-4 (IL-4) percentage of CD4 + and CD8 + T cells of mice via bacterial isolation, weighing, ELISA and flow cytometry, respectively. Results The splenic bacterial burden and spleen weight of the mice in group LI were mostly equivalent to the mice of group H. Moreover, Brucella-specific serum IgG, IFN-γ, IL-4, and the percentage of $ CD4^{+} $ and $ CD8^{+} $ T cells of the LI group mice were similar to those of the H group. In the subsequent challenge test, both vaccines conferred protective immunity to wild-type (WT) 2308 strain. In addition, the levels of IL-4 and IFN-γ, $ CD4^{+} $ and $ CD8^{+} $ T cells in these mice were similar to those of the mice in the H group. Conclusions Combined immunization with low dose live vaccine and inactivated vaccine allowed to reduce the live B. abortus A19 vaccine, dose with an equivalent protection of the high-dose live vaccine.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Brucellosis</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Combined immunization</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Live vaccine</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Inactivated vaccine</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhang, Yu-Zhuo</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Liu, Meng-Zhi</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhao, Hai-Long</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ren, Li-Song</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Liu, Bao-Shan</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">He, Sun</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Chen, Ze-Liang</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">BMC veterinary research</subfield><subfield code="d">London : BioMed Central, 2005</subfield><subfield code="g">18(2022), 1 vom: 02. 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author	He, Chuan-Yu
spellingShingle	He, Chuan-Yu misc Brucellosis misc Combined immunization misc Live vaccine misc Inactivated vaccine Combined immunization with inactivated vaccine reduces the dose of live B. abortus A19 vaccine
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topic_title	Combined immunization with inactivated vaccine reduces the dose of live B. abortus A19 vaccine Brucellosis (dpeaa)DE-He213 Combined immunization (dpeaa)DE-He213 Live vaccine (dpeaa)DE-He213 Inactivated vaccine (dpeaa)DE-He213
topic	misc Brucellosis misc Combined immunization misc Live vaccine misc Inactivated vaccine
topic_unstemmed	misc Brucellosis misc Combined immunization misc Live vaccine misc Inactivated vaccine
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title	Combined immunization with inactivated vaccine reduces the dose of live B. abortus A19 vaccine
ctrlnum	(DE-627)SPR050614088 (SPR)s12917-022-03229-0-e
title_full	Combined immunization with inactivated vaccine reduces the dose of live B. abortus A19 vaccine
author_sort	He, Chuan-Yu
journal	BMC veterinary research
journalStr	BMC veterinary research
lang_code	eng
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author_browse	He, Chuan-Yu Zhang, Yu-Zhuo Liu, Meng-Zhi Zhao, Hai-Long Ren, Li-Song Liu, Bao-Shan He, Sun Chen, Ze-Liang
container_volume	18
format_se	Elektronische Aufsätze
author-letter	He, Chuan-Yu
doi_str_mv	10.1186/s12917-022-03229-0
title_sort	combined immunization with inactivated vaccine reduces the dose of live b. abortus a19 vaccine
title_auth	Combined immunization with inactivated vaccine reduces the dose of live B. abortus A19 vaccine
abstract	Background Brucella spp. is an important zoonotic pathogen responsible for brucellosis in humans and animals. Brucella abortus A19 strain is a widespread vaccine in China. However, it has a drawback of residual virulence in animals and humans. Methods In this study, the BALB/c mice were inoculated with either 100 μL PBS(control group, C group), $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (I group), $ 10^{5} $ CFU/mL (low-dose group, L group) $ 10^{6} $ CFU/mL live B. abortus A19 strain (high-dose group, H group), or $ 10^{5} $ CFU/mL live B. abortus A19 strain combined with $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (LI group). Mice were challenged with B. abortus strain 2308 at 7 week post vaccination. Subsequently, the immune and protective efficacy of the vaccines were evaluated by measuring splenic bacterial burden, spleen weight, serum IgG, interferon-gamma (IFN-γ), interleukin-4 (IL-4) percentage of CD4 + and CD8 + T cells of mice via bacterial isolation, weighing, ELISA and flow cytometry, respectively. Results The splenic bacterial burden and spleen weight of the mice in group LI were mostly equivalent to the mice of group H. Moreover, Brucella-specific serum IgG, IFN-γ, IL-4, and the percentage of $ CD4^{+} $ and $ CD8^{+} $ T cells of the LI group mice were similar to those of the H group. In the subsequent challenge test, both vaccines conferred protective immunity to wild-type (WT) 2308 strain. In addition, the levels of IL-4 and IFN-γ, $ CD4^{+} $ and $ CD8^{+} $ T cells in these mice were similar to those of the mice in the H group. Conclusions Combined immunization with low dose live vaccine and inactivated vaccine allowed to reduce the live B. abortus A19 vaccine, dose with an equivalent protection of the high-dose live vaccine. © The Author(s) 2022
abstractGer	Background Brucella spp. is an important zoonotic pathogen responsible for brucellosis in humans and animals. Brucella abortus A19 strain is a widespread vaccine in China. However, it has a drawback of residual virulence in animals and humans. Methods In this study, the BALB/c mice were inoculated with either 100 μL PBS(control group, C group), $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (I group), $ 10^{5} $ CFU/mL (low-dose group, L group) $ 10^{6} $ CFU/mL live B. abortus A19 strain (high-dose group, H group), or $ 10^{5} $ CFU/mL live B. abortus A19 strain combined with $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (LI group). Mice were challenged with B. abortus strain 2308 at 7 week post vaccination. Subsequently, the immune and protective efficacy of the vaccines were evaluated by measuring splenic bacterial burden, spleen weight, serum IgG, interferon-gamma (IFN-γ), interleukin-4 (IL-4) percentage of CD4 + and CD8 + T cells of mice via bacterial isolation, weighing, ELISA and flow cytometry, respectively. Results The splenic bacterial burden and spleen weight of the mice in group LI were mostly equivalent to the mice of group H. Moreover, Brucella-specific serum IgG, IFN-γ, IL-4, and the percentage of $ CD4^{+} $ and $ CD8^{+} $ T cells of the LI group mice were similar to those of the H group. In the subsequent challenge test, both vaccines conferred protective immunity to wild-type (WT) 2308 strain. In addition, the levels of IL-4 and IFN-γ, $ CD4^{+} $ and $ CD8^{+} $ T cells in these mice were similar to those of the mice in the H group. Conclusions Combined immunization with low dose live vaccine and inactivated vaccine allowed to reduce the live B. abortus A19 vaccine, dose with an equivalent protection of the high-dose live vaccine. © The Author(s) 2022
abstract_unstemmed	Background Brucella spp. is an important zoonotic pathogen responsible for brucellosis in humans and animals. Brucella abortus A19 strain is a widespread vaccine in China. However, it has a drawback of residual virulence in animals and humans. Methods In this study, the BALB/c mice were inoculated with either 100 μL PBS(control group, C group), $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (I group), $ 10^{5} $ CFU/mL (low-dose group, L group) $ 10^{6} $ CFU/mL live B. abortus A19 strain (high-dose group, H group), or $ 10^{5} $ CFU/mL live B. abortus A19 strain combined with $ 10^{9} $ CFU/mL inactivated B. abortus A19 strain (LI group). Mice were challenged with B. abortus strain 2308 at 7 week post vaccination. Subsequently, the immune and protective efficacy of the vaccines were evaluated by measuring splenic bacterial burden, spleen weight, serum IgG, interferon-gamma (IFN-γ), interleukin-4 (IL-4) percentage of CD4 + and CD8 + T cells of mice via bacterial isolation, weighing, ELISA and flow cytometry, respectively. Results The splenic bacterial burden and spleen weight of the mice in group LI were mostly equivalent to the mice of group H. Moreover, Brucella-specific serum IgG, IFN-γ, IL-4, and the percentage of $ CD4^{+} $ and $ CD8^{+} $ T cells of the LI group mice were similar to those of the H group. In the subsequent challenge test, both vaccines conferred protective immunity to wild-type (WT) 2308 strain. In addition, the levels of IL-4 and IFN-γ, $ CD4^{+} $ and $ CD8^{+} $ T cells in these mice were similar to those of the mice in the H group. Conclusions Combined immunization with low dose live vaccine and inactivated vaccine allowed to reduce the live B. abortus A19 vaccine, dose with an equivalent protection of the high-dose live vaccine. © The Author(s) 2022
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container_issue	1
title_short	Combined immunization with inactivated vaccine reduces the dose of live B. abortus A19 vaccine
url	https://dx.doi.org/10.1186/s12917-022-03229-0
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author2	Zhang, Yu-Zhuo Liu, Meng-Zhi Zhao, Hai-Long Ren, Li-Song Liu, Bao-Shan He, Sun Chen, Ze-Liang
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up_date	2024-07-03T16:40:03.397Z
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Combined immunization with inactivated vaccine reduces the dose of live B. abortus A19 vaccine

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