Rebuilding hippocampus neural circuit with hADSC-derived neuron cells for treating ischemic stroke

Background Human adipose-derived stem cells (hADSCs) have been demonstrated to be a promising autologous stem cell source for treating various neuronal diseases. Our study indicated that hADSCs could be induced into neuron-like cells in a stepwise manner that are characterized by the positive expres...
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Autor*in:	Wang, Jian [verfasserIn] Hao, Rui Jiang, Tianfang Guo, Xuanxuan Zhou, Fei Cao, Limei Gao, Fengjuan Wang, Guangming Wang, Juan Ning, Ke Zhong, Chunlong Chen, Xu Huang, Ying Xu, Jun Gao, Shane

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Adipose-derived stem cells (ADSCs) hADSC-derived neuron-like cells (hADSC-NCs) Middle cerebral artery occlusion (MCAO) National Institute of Health Stroke Scale (NIHSS) Rogers scale system

Anmerkung:	© The Author(s) 2022

Übergeordnetes Werk:	Enthalten in: Cell & bioscience - London : BioMed Central, 2011, 12(2022), 1 vom: 04. Apr.
Übergeordnetes Werk:	volume:12 ; year:2022 ; number:1 ; day:04 ; month:04

Links:	Volltext

DOI / URN:	10.1186/s13578-022-00774-x

Katalog-ID:	SPR050617648

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520			\|a Background Human adipose-derived stem cells (hADSCs) have been demonstrated to be a promising autologous stem cell source for treating various neuronal diseases. Our study indicated that hADSCs could be induced into neuron-like cells in a stepwise manner that are characterized by the positive expression of MAP2, SYNAPSIN 1/2, NF-200, and vGLUT and electrophysiological activity. We first primed hADSCs into neuron-like cells (hADSC-NCs) and then intracerebrally transplanted them into MCAO reperfusion mice to further explore their in vivo survival, migration, integration, fate commitment and involvement in neural circuit rebuilding. Results The hADSC-NCs survived well and transformed into MAP2-positive, Iba1- or GFAP-negative cells in vivo while maintaining some proliferative ability, indicated by positive Ki67 staining after 4 weeks. hADSC-NCs could migrate to multiple brain regions, including the cortex, hippocampus, striatum, and hypothalamus, and further differentiate into mature neurons, as confirmed by action potential elicitation and postsynaptic currents. With the aid of a cell suicide system, hADSC-NCs were proven to have functionally integrated into the hippocampal memory circuit, where they contributed to spatial learning and memory rescue, as indicated by LTP improvement and subsequent GCV-induced relapse. In addition to infarction size shrinkage and movement improvement, MCAO-reperfused mice showed bidirectional immune modulation, including inhibition of the local proinflammatory factors IL-1α, IL-1β, IL-2, MIP-1β and promotion proinflammatory IP-10, MCP-1, and enhancement of the anti-inflammatory factors IL-15. Conclusion Overall, hADSC-NCs used as an intermediate autologous cell source for treating stroke can rebuild hippocampus neuronal circuits through cell replacement.
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650		4	\|a Middle cerebral artery occlusion (MCAO) \|7 (dpeaa)DE-He213
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allfields	10.1186/s13578-022-00774-x doi (DE-627)SPR050617648 (SPR)s13578-022-00774-x-e DE-627 ger DE-627 rakwb eng Wang, Jian verfasserin aut Rebuilding hippocampus neural circuit with hADSC-derived neuron cells for treating ischemic stroke 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Human adipose-derived stem cells (hADSCs) have been demonstrated to be a promising autologous stem cell source for treating various neuronal diseases. Our study indicated that hADSCs could be induced into neuron-like cells in a stepwise manner that are characterized by the positive expression of MAP2, SYNAPSIN 1/2, NF-200, and vGLUT and electrophysiological activity. We first primed hADSCs into neuron-like cells (hADSC-NCs) and then intracerebrally transplanted them into MCAO reperfusion mice to further explore their in vivo survival, migration, integration, fate commitment and involvement in neural circuit rebuilding. Results The hADSC-NCs survived well and transformed into MAP2-positive, Iba1- or GFAP-negative cells in vivo while maintaining some proliferative ability, indicated by positive Ki67 staining after 4 weeks. hADSC-NCs could migrate to multiple brain regions, including the cortex, hippocampus, striatum, and hypothalamus, and further differentiate into mature neurons, as confirmed by action potential elicitation and postsynaptic currents. With the aid of a cell suicide system, hADSC-NCs were proven to have functionally integrated into the hippocampal memory circuit, where they contributed to spatial learning and memory rescue, as indicated by LTP improvement and subsequent GCV-induced relapse. In addition to infarction size shrinkage and movement improvement, MCAO-reperfused mice showed bidirectional immune modulation, including inhibition of the local proinflammatory factors IL-1α, IL-1β, IL-2, MIP-1β and promotion proinflammatory IP-10, MCP-1, and enhancement of the anti-inflammatory factors IL-15. Conclusion Overall, hADSC-NCs used as an intermediate autologous cell source for treating stroke can rebuild hippocampus neuronal circuits through cell replacement. Adipose-derived stem cells (ADSCs) (dpeaa)DE-He213 hADSC-derived neuron-like cells (hADSC-NCs) (dpeaa)DE-He213 Middle cerebral artery occlusion (MCAO) (dpeaa)DE-He213 National Institute of Health Stroke Scale (NIHSS) (dpeaa)DE-He213 Rogers scale system (dpeaa)DE-He213 Hao, Rui aut Jiang, Tianfang aut Guo, Xuanxuan aut Zhou, Fei aut Cao, Limei aut Gao, Fengjuan aut Wang, Guangming aut Wang, Juan aut Ning, Ke aut Zhong, Chunlong aut Chen, Xu aut Huang, Ying aut Xu, Jun aut Gao, Shane aut Enthalten in Cell & bioscience London : BioMed Central, 2011 12(2022), 1 vom: 04. Apr. (DE-627)646079387 (DE-600)2593367-X 2045-3701 nnns volume:12 year:2022 number:1 day:04 month:04 https://dx.doi.org/10.1186/s13578-022-00774-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 1 04 04
spelling	10.1186/s13578-022-00774-x doi (DE-627)SPR050617648 (SPR)s13578-022-00774-x-e DE-627 ger DE-627 rakwb eng Wang, Jian verfasserin aut Rebuilding hippocampus neural circuit with hADSC-derived neuron cells for treating ischemic stroke 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Human adipose-derived stem cells (hADSCs) have been demonstrated to be a promising autologous stem cell source for treating various neuronal diseases. Our study indicated that hADSCs could be induced into neuron-like cells in a stepwise manner that are characterized by the positive expression of MAP2, SYNAPSIN 1/2, NF-200, and vGLUT and electrophysiological activity. We first primed hADSCs into neuron-like cells (hADSC-NCs) and then intracerebrally transplanted them into MCAO reperfusion mice to further explore their in vivo survival, migration, integration, fate commitment and involvement in neural circuit rebuilding. Results The hADSC-NCs survived well and transformed into MAP2-positive, Iba1- or GFAP-negative cells in vivo while maintaining some proliferative ability, indicated by positive Ki67 staining after 4 weeks. hADSC-NCs could migrate to multiple brain regions, including the cortex, hippocampus, striatum, and hypothalamus, and further differentiate into mature neurons, as confirmed by action potential elicitation and postsynaptic currents. With the aid of a cell suicide system, hADSC-NCs were proven to have functionally integrated into the hippocampal memory circuit, where they contributed to spatial learning and memory rescue, as indicated by LTP improvement and subsequent GCV-induced relapse. In addition to infarction size shrinkage and movement improvement, MCAO-reperfused mice showed bidirectional immune modulation, including inhibition of the local proinflammatory factors IL-1α, IL-1β, IL-2, MIP-1β and promotion proinflammatory IP-10, MCP-1, and enhancement of the anti-inflammatory factors IL-15. Conclusion Overall, hADSC-NCs used as an intermediate autologous cell source for treating stroke can rebuild hippocampus neuronal circuits through cell replacement. Adipose-derived stem cells (ADSCs) (dpeaa)DE-He213 hADSC-derived neuron-like cells (hADSC-NCs) (dpeaa)DE-He213 Middle cerebral artery occlusion (MCAO) (dpeaa)DE-He213 National Institute of Health Stroke Scale (NIHSS) (dpeaa)DE-He213 Rogers scale system (dpeaa)DE-He213 Hao, Rui aut Jiang, Tianfang aut Guo, Xuanxuan aut Zhou, Fei aut Cao, Limei aut Gao, Fengjuan aut Wang, Guangming aut Wang, Juan aut Ning, Ke aut Zhong, Chunlong aut Chen, Xu aut Huang, Ying aut Xu, Jun aut Gao, Shane aut Enthalten in Cell & bioscience London : BioMed Central, 2011 12(2022), 1 vom: 04. Apr. (DE-627)646079387 (DE-600)2593367-X 2045-3701 nnns volume:12 year:2022 number:1 day:04 month:04 https://dx.doi.org/10.1186/s13578-022-00774-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 1 04 04
allfields_unstemmed	10.1186/s13578-022-00774-x doi (DE-627)SPR050617648 (SPR)s13578-022-00774-x-e DE-627 ger DE-627 rakwb eng Wang, Jian verfasserin aut Rebuilding hippocampus neural circuit with hADSC-derived neuron cells for treating ischemic stroke 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Human adipose-derived stem cells (hADSCs) have been demonstrated to be a promising autologous stem cell source for treating various neuronal diseases. Our study indicated that hADSCs could be induced into neuron-like cells in a stepwise manner that are characterized by the positive expression of MAP2, SYNAPSIN 1/2, NF-200, and vGLUT and electrophysiological activity. We first primed hADSCs into neuron-like cells (hADSC-NCs) and then intracerebrally transplanted them into MCAO reperfusion mice to further explore their in vivo survival, migration, integration, fate commitment and involvement in neural circuit rebuilding. Results The hADSC-NCs survived well and transformed into MAP2-positive, Iba1- or GFAP-negative cells in vivo while maintaining some proliferative ability, indicated by positive Ki67 staining after 4 weeks. hADSC-NCs could migrate to multiple brain regions, including the cortex, hippocampus, striatum, and hypothalamus, and further differentiate into mature neurons, as confirmed by action potential elicitation and postsynaptic currents. With the aid of a cell suicide system, hADSC-NCs were proven to have functionally integrated into the hippocampal memory circuit, where they contributed to spatial learning and memory rescue, as indicated by LTP improvement and subsequent GCV-induced relapse. In addition to infarction size shrinkage and movement improvement, MCAO-reperfused mice showed bidirectional immune modulation, including inhibition of the local proinflammatory factors IL-1α, IL-1β, IL-2, MIP-1β and promotion proinflammatory IP-10, MCP-1, and enhancement of the anti-inflammatory factors IL-15. Conclusion Overall, hADSC-NCs used as an intermediate autologous cell source for treating stroke can rebuild hippocampus neuronal circuits through cell replacement. Adipose-derived stem cells (ADSCs) (dpeaa)DE-He213 hADSC-derived neuron-like cells (hADSC-NCs) (dpeaa)DE-He213 Middle cerebral artery occlusion (MCAO) (dpeaa)DE-He213 National Institute of Health Stroke Scale (NIHSS) (dpeaa)DE-He213 Rogers scale system (dpeaa)DE-He213 Hao, Rui aut Jiang, Tianfang aut Guo, Xuanxuan aut Zhou, Fei aut Cao, Limei aut Gao, Fengjuan aut Wang, Guangming aut Wang, Juan aut Ning, Ke aut Zhong, Chunlong aut Chen, Xu aut Huang, Ying aut Xu, Jun aut Gao, Shane aut Enthalten in Cell & bioscience London : BioMed Central, 2011 12(2022), 1 vom: 04. Apr. (DE-627)646079387 (DE-600)2593367-X 2045-3701 nnns volume:12 year:2022 number:1 day:04 month:04 https://dx.doi.org/10.1186/s13578-022-00774-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 1 04 04
allfieldsGer	10.1186/s13578-022-00774-x doi (DE-627)SPR050617648 (SPR)s13578-022-00774-x-e DE-627 ger DE-627 rakwb eng Wang, Jian verfasserin aut Rebuilding hippocampus neural circuit with hADSC-derived neuron cells for treating ischemic stroke 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Human adipose-derived stem cells (hADSCs) have been demonstrated to be a promising autologous stem cell source for treating various neuronal diseases. Our study indicated that hADSCs could be induced into neuron-like cells in a stepwise manner that are characterized by the positive expression of MAP2, SYNAPSIN 1/2, NF-200, and vGLUT and electrophysiological activity. We first primed hADSCs into neuron-like cells (hADSC-NCs) and then intracerebrally transplanted them into MCAO reperfusion mice to further explore their in vivo survival, migration, integration, fate commitment and involvement in neural circuit rebuilding. Results The hADSC-NCs survived well and transformed into MAP2-positive, Iba1- or GFAP-negative cells in vivo while maintaining some proliferative ability, indicated by positive Ki67 staining after 4 weeks. hADSC-NCs could migrate to multiple brain regions, including the cortex, hippocampus, striatum, and hypothalamus, and further differentiate into mature neurons, as confirmed by action potential elicitation and postsynaptic currents. With the aid of a cell suicide system, hADSC-NCs were proven to have functionally integrated into the hippocampal memory circuit, where they contributed to spatial learning and memory rescue, as indicated by LTP improvement and subsequent GCV-induced relapse. In addition to infarction size shrinkage and movement improvement, MCAO-reperfused mice showed bidirectional immune modulation, including inhibition of the local proinflammatory factors IL-1α, IL-1β, IL-2, MIP-1β and promotion proinflammatory IP-10, MCP-1, and enhancement of the anti-inflammatory factors IL-15. Conclusion Overall, hADSC-NCs used as an intermediate autologous cell source for treating stroke can rebuild hippocampus neuronal circuits through cell replacement. Adipose-derived stem cells (ADSCs) (dpeaa)DE-He213 hADSC-derived neuron-like cells (hADSC-NCs) (dpeaa)DE-He213 Middle cerebral artery occlusion (MCAO) (dpeaa)DE-He213 National Institute of Health Stroke Scale (NIHSS) (dpeaa)DE-He213 Rogers scale system (dpeaa)DE-He213 Hao, Rui aut Jiang, Tianfang aut Guo, Xuanxuan aut Zhou, Fei aut Cao, Limei aut Gao, Fengjuan aut Wang, Guangming aut Wang, Juan aut Ning, Ke aut Zhong, Chunlong aut Chen, Xu aut Huang, Ying aut Xu, Jun aut Gao, Shane aut Enthalten in Cell & bioscience London : BioMed Central, 2011 12(2022), 1 vom: 04. Apr. (DE-627)646079387 (DE-600)2593367-X 2045-3701 nnns volume:12 year:2022 number:1 day:04 month:04 https://dx.doi.org/10.1186/s13578-022-00774-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 1 04 04
allfieldsSound	10.1186/s13578-022-00774-x doi (DE-627)SPR050617648 (SPR)s13578-022-00774-x-e DE-627 ger DE-627 rakwb eng Wang, Jian verfasserin aut Rebuilding hippocampus neural circuit with hADSC-derived neuron cells for treating ischemic stroke 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Human adipose-derived stem cells (hADSCs) have been demonstrated to be a promising autologous stem cell source for treating various neuronal diseases. Our study indicated that hADSCs could be induced into neuron-like cells in a stepwise manner that are characterized by the positive expression of MAP2, SYNAPSIN 1/2, NF-200, and vGLUT and electrophysiological activity. We first primed hADSCs into neuron-like cells (hADSC-NCs) and then intracerebrally transplanted them into MCAO reperfusion mice to further explore their in vivo survival, migration, integration, fate commitment and involvement in neural circuit rebuilding. Results The hADSC-NCs survived well and transformed into MAP2-positive, Iba1- or GFAP-negative cells in vivo while maintaining some proliferative ability, indicated by positive Ki67 staining after 4 weeks. hADSC-NCs could migrate to multiple brain regions, including the cortex, hippocampus, striatum, and hypothalamus, and further differentiate into mature neurons, as confirmed by action potential elicitation and postsynaptic currents. With the aid of a cell suicide system, hADSC-NCs were proven to have functionally integrated into the hippocampal memory circuit, where they contributed to spatial learning and memory rescue, as indicated by LTP improvement and subsequent GCV-induced relapse. In addition to infarction size shrinkage and movement improvement, MCAO-reperfused mice showed bidirectional immune modulation, including inhibition of the local proinflammatory factors IL-1α, IL-1β, IL-2, MIP-1β and promotion proinflammatory IP-10, MCP-1, and enhancement of the anti-inflammatory factors IL-15. Conclusion Overall, hADSC-NCs used as an intermediate autologous cell source for treating stroke can rebuild hippocampus neuronal circuits through cell replacement. Adipose-derived stem cells (ADSCs) (dpeaa)DE-He213 hADSC-derived neuron-like cells (hADSC-NCs) (dpeaa)DE-He213 Middle cerebral artery occlusion (MCAO) (dpeaa)DE-He213 National Institute of Health Stroke Scale (NIHSS) (dpeaa)DE-He213 Rogers scale system (dpeaa)DE-He213 Hao, Rui aut Jiang, Tianfang aut Guo, Xuanxuan aut Zhou, Fei aut Cao, Limei aut Gao, Fengjuan aut Wang, Guangming aut Wang, Juan aut Ning, Ke aut Zhong, Chunlong aut Chen, Xu aut Huang, Ying aut Xu, Jun aut Gao, Shane aut Enthalten in Cell & bioscience London : BioMed Central, 2011 12(2022), 1 vom: 04. Apr. (DE-627)646079387 (DE-600)2593367-X 2045-3701 nnns volume:12 year:2022 number:1 day:04 month:04 https://dx.doi.org/10.1186/s13578-022-00774-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022 1 04 04
language	English
source	Enthalten in Cell & bioscience 12(2022), 1 vom: 04. Apr. volume:12 year:2022 number:1 day:04 month:04
sourceStr	Enthalten in Cell & bioscience 12(2022), 1 vom: 04. Apr. volume:12 year:2022 number:1 day:04 month:04
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Adipose-derived stem cells (ADSCs) hADSC-derived neuron-like cells (hADSC-NCs) Middle cerebral artery occlusion (MCAO) National Institute of Health Stroke Scale (NIHSS) Rogers scale system
isfreeaccess_bool	true
container_title	Cell & bioscience
authorswithroles_txt_mv	Wang, Jian @@aut@@ Hao, Rui @@aut@@ Jiang, Tianfang @@aut@@ Guo, Xuanxuan @@aut@@ Zhou, Fei @@aut@@ Cao, Limei @@aut@@ Gao, Fengjuan @@aut@@ Wang, Guangming @@aut@@ Wang, Juan @@aut@@ Ning, Ke @@aut@@ Zhong, Chunlong @@aut@@ Chen, Xu @@aut@@ Huang, Ying @@aut@@ Xu, Jun @@aut@@ Gao, Shane @@aut@@
publishDateDaySort_date	2022-04-04T00:00:00Z
hierarchy_top_id	646079387
id	SPR050617648
language_de	englisch
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Our study indicated that hADSCs could be induced into neuron-like cells in a stepwise manner that are characterized by the positive expression of MAP2, SYNAPSIN 1/2, NF-200, and vGLUT and electrophysiological activity. We first primed hADSCs into neuron-like cells (hADSC-NCs) and then intracerebrally transplanted them into MCAO reperfusion mice to further explore their in vivo survival, migration, integration, fate commitment and involvement in neural circuit rebuilding. Results The hADSC-NCs survived well and transformed into MAP2-positive, Iba1- or GFAP-negative cells in vivo while maintaining some proliferative ability, indicated by positive Ki67 staining after 4 weeks. hADSC-NCs could migrate to multiple brain regions, including the cortex, hippocampus, striatum, and hypothalamus, and further differentiate into mature neurons, as confirmed by action potential elicitation and postsynaptic currents. With the aid of a cell suicide system, hADSC-NCs were proven to have functionally integrated into the hippocampal memory circuit, where they contributed to spatial learning and memory rescue, as indicated by LTP improvement and subsequent GCV-induced relapse. In addition to infarction size shrinkage and movement improvement, MCAO-reperfused mice showed bidirectional immune modulation, including inhibition of the local proinflammatory factors IL-1α, IL-1β, IL-2, MIP-1β and promotion proinflammatory IP-10, MCP-1, and enhancement of the anti-inflammatory factors IL-15. 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author	Wang, Jian
spellingShingle	Wang, Jian misc Adipose-derived stem cells (ADSCs) misc hADSC-derived neuron-like cells (hADSC-NCs) misc Middle cerebral artery occlusion (MCAO) misc National Institute of Health Stroke Scale (NIHSS) misc Rogers scale system Rebuilding hippocampus neural circuit with hADSC-derived neuron cells for treating ischemic stroke
authorStr	Wang, Jian
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topic_title	Rebuilding hippocampus neural circuit with hADSC-derived neuron cells for treating ischemic stroke Adipose-derived stem cells (ADSCs) (dpeaa)DE-He213 hADSC-derived neuron-like cells (hADSC-NCs) (dpeaa)DE-He213 Middle cerebral artery occlusion (MCAO) (dpeaa)DE-He213 National Institute of Health Stroke Scale (NIHSS) (dpeaa)DE-He213 Rogers scale system (dpeaa)DE-He213
topic	misc Adipose-derived stem cells (ADSCs) misc hADSC-derived neuron-like cells (hADSC-NCs) misc Middle cerebral artery occlusion (MCAO) misc National Institute of Health Stroke Scale (NIHSS) misc Rogers scale system
topic_unstemmed	misc Adipose-derived stem cells (ADSCs) misc hADSC-derived neuron-like cells (hADSC-NCs) misc Middle cerebral artery occlusion (MCAO) misc National Institute of Health Stroke Scale (NIHSS) misc Rogers scale system
topic_browse	misc Adipose-derived stem cells (ADSCs) misc hADSC-derived neuron-like cells (hADSC-NCs) misc Middle cerebral artery occlusion (MCAO) misc National Institute of Health Stroke Scale (NIHSS) misc Rogers scale system
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title	Rebuilding hippocampus neural circuit with hADSC-derived neuron cells for treating ischemic stroke
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title_full	Rebuilding hippocampus neural circuit with hADSC-derived neuron cells for treating ischemic stroke
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author_browse	Wang, Jian Hao, Rui Jiang, Tianfang Guo, Xuanxuan Zhou, Fei Cao, Limei Gao, Fengjuan Wang, Guangming Wang, Juan Ning, Ke Zhong, Chunlong Chen, Xu Huang, Ying Xu, Jun Gao, Shane
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author-letter	Wang, Jian
doi_str_mv	10.1186/s13578-022-00774-x
title_sort	rebuilding hippocampus neural circuit with hadsc-derived neuron cells for treating ischemic stroke
title_auth	Rebuilding hippocampus neural circuit with hADSC-derived neuron cells for treating ischemic stroke
abstract	Background Human adipose-derived stem cells (hADSCs) have been demonstrated to be a promising autologous stem cell source for treating various neuronal diseases. Our study indicated that hADSCs could be induced into neuron-like cells in a stepwise manner that are characterized by the positive expression of MAP2, SYNAPSIN 1/2, NF-200, and vGLUT and electrophysiological activity. We first primed hADSCs into neuron-like cells (hADSC-NCs) and then intracerebrally transplanted them into MCAO reperfusion mice to further explore their in vivo survival, migration, integration, fate commitment and involvement in neural circuit rebuilding. Results The hADSC-NCs survived well and transformed into MAP2-positive, Iba1- or GFAP-negative cells in vivo while maintaining some proliferative ability, indicated by positive Ki67 staining after 4 weeks. hADSC-NCs could migrate to multiple brain regions, including the cortex, hippocampus, striatum, and hypothalamus, and further differentiate into mature neurons, as confirmed by action potential elicitation and postsynaptic currents. With the aid of a cell suicide system, hADSC-NCs were proven to have functionally integrated into the hippocampal memory circuit, where they contributed to spatial learning and memory rescue, as indicated by LTP improvement and subsequent GCV-induced relapse. In addition to infarction size shrinkage and movement improvement, MCAO-reperfused mice showed bidirectional immune modulation, including inhibition of the local proinflammatory factors IL-1α, IL-1β, IL-2, MIP-1β and promotion proinflammatory IP-10, MCP-1, and enhancement of the anti-inflammatory factors IL-15. Conclusion Overall, hADSC-NCs used as an intermediate autologous cell source for treating stroke can rebuild hippocampus neuronal circuits through cell replacement. © The Author(s) 2022
abstractGer	Background Human adipose-derived stem cells (hADSCs) have been demonstrated to be a promising autologous stem cell source for treating various neuronal diseases. Our study indicated that hADSCs could be induced into neuron-like cells in a stepwise manner that are characterized by the positive expression of MAP2, SYNAPSIN 1/2, NF-200, and vGLUT and electrophysiological activity. We first primed hADSCs into neuron-like cells (hADSC-NCs) and then intracerebrally transplanted them into MCAO reperfusion mice to further explore their in vivo survival, migration, integration, fate commitment and involvement in neural circuit rebuilding. Results The hADSC-NCs survived well and transformed into MAP2-positive, Iba1- or GFAP-negative cells in vivo while maintaining some proliferative ability, indicated by positive Ki67 staining after 4 weeks. hADSC-NCs could migrate to multiple brain regions, including the cortex, hippocampus, striatum, and hypothalamus, and further differentiate into mature neurons, as confirmed by action potential elicitation and postsynaptic currents. With the aid of a cell suicide system, hADSC-NCs were proven to have functionally integrated into the hippocampal memory circuit, where they contributed to spatial learning and memory rescue, as indicated by LTP improvement and subsequent GCV-induced relapse. In addition to infarction size shrinkage and movement improvement, MCAO-reperfused mice showed bidirectional immune modulation, including inhibition of the local proinflammatory factors IL-1α, IL-1β, IL-2, MIP-1β and promotion proinflammatory IP-10, MCP-1, and enhancement of the anti-inflammatory factors IL-15. Conclusion Overall, hADSC-NCs used as an intermediate autologous cell source for treating stroke can rebuild hippocampus neuronal circuits through cell replacement. © The Author(s) 2022
abstract_unstemmed	Background Human adipose-derived stem cells (hADSCs) have been demonstrated to be a promising autologous stem cell source for treating various neuronal diseases. Our study indicated that hADSCs could be induced into neuron-like cells in a stepwise manner that are characterized by the positive expression of MAP2, SYNAPSIN 1/2, NF-200, and vGLUT and electrophysiological activity. We first primed hADSCs into neuron-like cells (hADSC-NCs) and then intracerebrally transplanted them into MCAO reperfusion mice to further explore their in vivo survival, migration, integration, fate commitment and involvement in neural circuit rebuilding. Results The hADSC-NCs survived well and transformed into MAP2-positive, Iba1- or GFAP-negative cells in vivo while maintaining some proliferative ability, indicated by positive Ki67 staining after 4 weeks. hADSC-NCs could migrate to multiple brain regions, including the cortex, hippocampus, striatum, and hypothalamus, and further differentiate into mature neurons, as confirmed by action potential elicitation and postsynaptic currents. With the aid of a cell suicide system, hADSC-NCs were proven to have functionally integrated into the hippocampal memory circuit, where they contributed to spatial learning and memory rescue, as indicated by LTP improvement and subsequent GCV-induced relapse. In addition to infarction size shrinkage and movement improvement, MCAO-reperfused mice showed bidirectional immune modulation, including inhibition of the local proinflammatory factors IL-1α, IL-1β, IL-2, MIP-1β and promotion proinflammatory IP-10, MCP-1, and enhancement of the anti-inflammatory factors IL-15. Conclusion Overall, hADSC-NCs used as an intermediate autologous cell source for treating stroke can rebuild hippocampus neuronal circuits through cell replacement. © The Author(s) 2022
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container_issue	1
title_short	Rebuilding hippocampus neural circuit with hADSC-derived neuron cells for treating ischemic stroke
url	https://dx.doi.org/10.1186/s13578-022-00774-x
remote_bool	true
author2	Hao, Rui Jiang, Tianfang Guo, Xuanxuan Zhou, Fei Cao, Limei Gao, Fengjuan Wang, Guangming Wang, Juan Ning, Ke Zhong, Chunlong Chen, Xu Huang, Ying Xu, Jun Gao, Shane
author2Str	Hao, Rui Jiang, Tianfang Guo, Xuanxuan Zhou, Fei Cao, Limei Gao, Fengjuan Wang, Guangming Wang, Juan Ning, Ke Zhong, Chunlong Chen, Xu Huang, Ying Xu, Jun Gao, Shane
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up_date	2024-07-03T16:41:25.168Z
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