Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers
Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 he...
Ausführliche Beschreibung
Autor*in: |
Jiang, Daixi [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2022 |
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Schlagwörter: |
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Anmerkung: |
© The Author(s) 2022 |
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Übergeordnetes Werk: |
Enthalten in: BMC immunology - London : BioMed Central, 2000, 23(2022), 1 vom: 20. Apr. |
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Übergeordnetes Werk: |
volume:23 ; year:2022 ; number:1 ; day:20 ; month:04 |
Links: |
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DOI / URN: |
10.1186/s12865-022-00488-2 |
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Katalog-ID: |
SPR050653776 |
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245 | 1 | 0 | |a Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers |
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520 | |a Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of $ CD8^{+} $ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry. Results There were no significant differences in the baseline characteristics between the 31 HBV carriers and the 23 sex- and age-matched HCs. $ CD8^{+} $ T cells exhibited higher levels of inhibitory receptors (TIM3 and PD1) in the HBV carriers than in the HCs (P < 0.05); in particular, Tfc cells ($ CXCR5^{+} %$ CD25^{−} $) expressed higher levels of TIM3 and PD1 than non-Tfc cells in the HBV carriers. In addition, among the subsets of Tc cells, the Tc17 ($ CXCR5^{−} %$ CD25^{−} %$ CCR6^{+} $) subset displayed increased expression of TIM3 and LAG3 in the HBV carriers. Our findings further showed that $ CD8^{+} $ T cells produced lower levels of IFN-γ, TNF-α, and Granzyme B. Paired analysis of the Tfc subset and the Tc subset indicated that higher levels of cytokines (IFN-γ and TNF-α) were produced by the Tfc subset in the HBV carriers. Among the Tc subsets, the Tc17 subset produced lower levels of cytokines. Conclusion The Tfc subset exhibited an enhanced exhausted phenotype but possessed some functional properties during chronic HBV infection, while the Tc subset showed a lower functional level. The identification of these unique subsets may provide a potential immunotherapeutic target in chronic HBV infection. | ||
650 | 4 | |a HBV |7 (dpeaa)DE-He213 | |
650 | 4 | |a CD8 |7 (dpeaa)DE-He213 | |
650 | 4 | |a T cell |7 (dpeaa)DE-He213 | |
650 | 4 | |a CXCR5 |7 (dpeaa)DE-He213 | |
650 | 4 | |a Exhaustion |7 (dpeaa)DE-He213 | |
650 | 4 | |a Flow cytometry |7 (dpeaa)DE-He213 | |
700 | 1 | |a Chen, Can |4 aut | |
700 | 1 | |a Yan, Danying |4 aut | |
700 | 1 | |a Zhang, Xiaobao |4 aut | |
700 | 1 | |a Liu, Xiaoxiao |4 aut | |
700 | 1 | |a Yan, Dong |4 aut | |
700 | 1 | |a Cui, Dawei |4 aut | |
700 | 1 | |a Yang, Shigui |4 aut | |
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10.1186/s12865-022-00488-2 doi (DE-627)SPR050653776 (SPR)s12865-022-00488-2-e DE-627 ger DE-627 rakwb eng Jiang, Daixi verfasserin aut Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of $ CD8^{+} $ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry. Results There were no significant differences in the baseline characteristics between the 31 HBV carriers and the 23 sex- and age-matched HCs. $ CD8^{+} $ T cells exhibited higher levels of inhibitory receptors (TIM3 and PD1) in the HBV carriers than in the HCs (P < 0.05); in particular, Tfc cells ($ CXCR5^{+} %$ CD25^{−} $) expressed higher levels of TIM3 and PD1 than non-Tfc cells in the HBV carriers. In addition, among the subsets of Tc cells, the Tc17 ($ CXCR5^{−} %$ CD25^{−} %$ CCR6^{+} $) subset displayed increased expression of TIM3 and LAG3 in the HBV carriers. Our findings further showed that $ CD8^{+} $ T cells produced lower levels of IFN-γ, TNF-α, and Granzyme B. Paired analysis of the Tfc subset and the Tc subset indicated that higher levels of cytokines (IFN-γ and TNF-α) were produced by the Tfc subset in the HBV carriers. Among the Tc subsets, the Tc17 subset produced lower levels of cytokines. Conclusion The Tfc subset exhibited an enhanced exhausted phenotype but possessed some functional properties during chronic HBV infection, while the Tc subset showed a lower functional level. The identification of these unique subsets may provide a potential immunotherapeutic target in chronic HBV infection. HBV (dpeaa)DE-He213 CD8 (dpeaa)DE-He213 T cell (dpeaa)DE-He213 CXCR5 (dpeaa)DE-He213 Exhaustion (dpeaa)DE-He213 Flow cytometry (dpeaa)DE-He213 Chen, Can aut Yan, Danying aut Zhang, Xiaobao aut Liu, Xiaoxiao aut Yan, Dong aut Cui, Dawei aut Yang, Shigui aut Enthalten in BMC immunology London : BioMed Central, 2000 23(2022), 1 vom: 20. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:23 year:2022 number:1 day:20 month:04 https://dx.doi.org/10.1186/s12865-022-00488-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 1 20 04 |
spelling |
10.1186/s12865-022-00488-2 doi (DE-627)SPR050653776 (SPR)s12865-022-00488-2-e DE-627 ger DE-627 rakwb eng Jiang, Daixi verfasserin aut Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of $ CD8^{+} $ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry. Results There were no significant differences in the baseline characteristics between the 31 HBV carriers and the 23 sex- and age-matched HCs. $ CD8^{+} $ T cells exhibited higher levels of inhibitory receptors (TIM3 and PD1) in the HBV carriers than in the HCs (P < 0.05); in particular, Tfc cells ($ CXCR5^{+} %$ CD25^{−} $) expressed higher levels of TIM3 and PD1 than non-Tfc cells in the HBV carriers. In addition, among the subsets of Tc cells, the Tc17 ($ CXCR5^{−} %$ CD25^{−} %$ CCR6^{+} $) subset displayed increased expression of TIM3 and LAG3 in the HBV carriers. Our findings further showed that $ CD8^{+} $ T cells produced lower levels of IFN-γ, TNF-α, and Granzyme B. Paired analysis of the Tfc subset and the Tc subset indicated that higher levels of cytokines (IFN-γ and TNF-α) were produced by the Tfc subset in the HBV carriers. Among the Tc subsets, the Tc17 subset produced lower levels of cytokines. Conclusion The Tfc subset exhibited an enhanced exhausted phenotype but possessed some functional properties during chronic HBV infection, while the Tc subset showed a lower functional level. The identification of these unique subsets may provide a potential immunotherapeutic target in chronic HBV infection. HBV (dpeaa)DE-He213 CD8 (dpeaa)DE-He213 T cell (dpeaa)DE-He213 CXCR5 (dpeaa)DE-He213 Exhaustion (dpeaa)DE-He213 Flow cytometry (dpeaa)DE-He213 Chen, Can aut Yan, Danying aut Zhang, Xiaobao aut Liu, Xiaoxiao aut Yan, Dong aut Cui, Dawei aut Yang, Shigui aut Enthalten in BMC immunology London : BioMed Central, 2000 23(2022), 1 vom: 20. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:23 year:2022 number:1 day:20 month:04 https://dx.doi.org/10.1186/s12865-022-00488-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 1 20 04 |
allfields_unstemmed |
10.1186/s12865-022-00488-2 doi (DE-627)SPR050653776 (SPR)s12865-022-00488-2-e DE-627 ger DE-627 rakwb eng Jiang, Daixi verfasserin aut Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of $ CD8^{+} $ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry. Results There were no significant differences in the baseline characteristics between the 31 HBV carriers and the 23 sex- and age-matched HCs. $ CD8^{+} $ T cells exhibited higher levels of inhibitory receptors (TIM3 and PD1) in the HBV carriers than in the HCs (P < 0.05); in particular, Tfc cells ($ CXCR5^{+} %$ CD25^{−} $) expressed higher levels of TIM3 and PD1 than non-Tfc cells in the HBV carriers. In addition, among the subsets of Tc cells, the Tc17 ($ CXCR5^{−} %$ CD25^{−} %$ CCR6^{+} $) subset displayed increased expression of TIM3 and LAG3 in the HBV carriers. Our findings further showed that $ CD8^{+} $ T cells produced lower levels of IFN-γ, TNF-α, and Granzyme B. Paired analysis of the Tfc subset and the Tc subset indicated that higher levels of cytokines (IFN-γ and TNF-α) were produced by the Tfc subset in the HBV carriers. Among the Tc subsets, the Tc17 subset produced lower levels of cytokines. Conclusion The Tfc subset exhibited an enhanced exhausted phenotype but possessed some functional properties during chronic HBV infection, while the Tc subset showed a lower functional level. The identification of these unique subsets may provide a potential immunotherapeutic target in chronic HBV infection. HBV (dpeaa)DE-He213 CD8 (dpeaa)DE-He213 T cell (dpeaa)DE-He213 CXCR5 (dpeaa)DE-He213 Exhaustion (dpeaa)DE-He213 Flow cytometry (dpeaa)DE-He213 Chen, Can aut Yan, Danying aut Zhang, Xiaobao aut Liu, Xiaoxiao aut Yan, Dong aut Cui, Dawei aut Yang, Shigui aut Enthalten in BMC immunology London : BioMed Central, 2000 23(2022), 1 vom: 20. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:23 year:2022 number:1 day:20 month:04 https://dx.doi.org/10.1186/s12865-022-00488-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 1 20 04 |
allfieldsGer |
10.1186/s12865-022-00488-2 doi (DE-627)SPR050653776 (SPR)s12865-022-00488-2-e DE-627 ger DE-627 rakwb eng Jiang, Daixi verfasserin aut Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of $ CD8^{+} $ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry. Results There were no significant differences in the baseline characteristics between the 31 HBV carriers and the 23 sex- and age-matched HCs. $ CD8^{+} $ T cells exhibited higher levels of inhibitory receptors (TIM3 and PD1) in the HBV carriers than in the HCs (P < 0.05); in particular, Tfc cells ($ CXCR5^{+} %$ CD25^{−} $) expressed higher levels of TIM3 and PD1 than non-Tfc cells in the HBV carriers. In addition, among the subsets of Tc cells, the Tc17 ($ CXCR5^{−} %$ CD25^{−} %$ CCR6^{+} $) subset displayed increased expression of TIM3 and LAG3 in the HBV carriers. Our findings further showed that $ CD8^{+} $ T cells produced lower levels of IFN-γ, TNF-α, and Granzyme B. Paired analysis of the Tfc subset and the Tc subset indicated that higher levels of cytokines (IFN-γ and TNF-α) were produced by the Tfc subset in the HBV carriers. Among the Tc subsets, the Tc17 subset produced lower levels of cytokines. Conclusion The Tfc subset exhibited an enhanced exhausted phenotype but possessed some functional properties during chronic HBV infection, while the Tc subset showed a lower functional level. The identification of these unique subsets may provide a potential immunotherapeutic target in chronic HBV infection. HBV (dpeaa)DE-He213 CD8 (dpeaa)DE-He213 T cell (dpeaa)DE-He213 CXCR5 (dpeaa)DE-He213 Exhaustion (dpeaa)DE-He213 Flow cytometry (dpeaa)DE-He213 Chen, Can aut Yan, Danying aut Zhang, Xiaobao aut Liu, Xiaoxiao aut Yan, Dong aut Cui, Dawei aut Yang, Shigui aut Enthalten in BMC immunology London : BioMed Central, 2000 23(2022), 1 vom: 20. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:23 year:2022 number:1 day:20 month:04 https://dx.doi.org/10.1186/s12865-022-00488-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 1 20 04 |
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10.1186/s12865-022-00488-2 doi (DE-627)SPR050653776 (SPR)s12865-022-00488-2-e DE-627 ger DE-627 rakwb eng Jiang, Daixi verfasserin aut Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of $ CD8^{+} $ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry. Results There were no significant differences in the baseline characteristics between the 31 HBV carriers and the 23 sex- and age-matched HCs. $ CD8^{+} $ T cells exhibited higher levels of inhibitory receptors (TIM3 and PD1) in the HBV carriers than in the HCs (P < 0.05); in particular, Tfc cells ($ CXCR5^{+} %$ CD25^{−} $) expressed higher levels of TIM3 and PD1 than non-Tfc cells in the HBV carriers. In addition, among the subsets of Tc cells, the Tc17 ($ CXCR5^{−} %$ CD25^{−} %$ CCR6^{+} $) subset displayed increased expression of TIM3 and LAG3 in the HBV carriers. Our findings further showed that $ CD8^{+} $ T cells produced lower levels of IFN-γ, TNF-α, and Granzyme B. Paired analysis of the Tfc subset and the Tc subset indicated that higher levels of cytokines (IFN-γ and TNF-α) were produced by the Tfc subset in the HBV carriers. Among the Tc subsets, the Tc17 subset produced lower levels of cytokines. Conclusion The Tfc subset exhibited an enhanced exhausted phenotype but possessed some functional properties during chronic HBV infection, while the Tc subset showed a lower functional level. The identification of these unique subsets may provide a potential immunotherapeutic target in chronic HBV infection. HBV (dpeaa)DE-He213 CD8 (dpeaa)DE-He213 T cell (dpeaa)DE-He213 CXCR5 (dpeaa)DE-He213 Exhaustion (dpeaa)DE-He213 Flow cytometry (dpeaa)DE-He213 Chen, Can aut Yan, Danying aut Zhang, Xiaobao aut Liu, Xiaoxiao aut Yan, Dong aut Cui, Dawei aut Yang, Shigui aut Enthalten in BMC immunology London : BioMed Central, 2000 23(2022), 1 vom: 20. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:23 year:2022 number:1 day:20 month:04 https://dx.doi.org/10.1186/s12865-022-00488-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 1 20 04 |
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Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers |
ctrlnum |
(DE-627)SPR050653776 (SPR)s12865-022-00488-2-e |
title_full |
Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers |
author_sort |
Jiang, Daixi |
journal |
BMC immunology |
journalStr |
BMC immunology |
lang_code |
eng |
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true |
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marc |
publishDateSort |
2022 |
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txt |
author_browse |
Jiang, Daixi Chen, Can Yan, Danying Zhang, Xiaobao Liu, Xiaoxiao Yan, Dong Cui, Dawei Yang, Shigui |
container_volume |
23 |
format_se |
Elektronische Aufsätze |
author-letter |
Jiang, Daixi |
doi_str_mv |
10.1186/s12865-022-00488-2 |
title_sort |
exhausted phenotype of circulating $ cd8^{+} $ t cell subsets in hepatitis b virus carriers |
title_auth |
Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers |
abstract |
Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of $ CD8^{+} $ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry. Results There were no significant differences in the baseline characteristics between the 31 HBV carriers and the 23 sex- and age-matched HCs. $ CD8^{+} $ T cells exhibited higher levels of inhibitory receptors (TIM3 and PD1) in the HBV carriers than in the HCs (P < 0.05); in particular, Tfc cells ($ CXCR5^{+} %$ CD25^{−} $) expressed higher levels of TIM3 and PD1 than non-Tfc cells in the HBV carriers. In addition, among the subsets of Tc cells, the Tc17 ($ CXCR5^{−} %$ CD25^{−} %$ CCR6^{+} $) subset displayed increased expression of TIM3 and LAG3 in the HBV carriers. Our findings further showed that $ CD8^{+} $ T cells produced lower levels of IFN-γ, TNF-α, and Granzyme B. Paired analysis of the Tfc subset and the Tc subset indicated that higher levels of cytokines (IFN-γ and TNF-α) were produced by the Tfc subset in the HBV carriers. Among the Tc subsets, the Tc17 subset produced lower levels of cytokines. Conclusion The Tfc subset exhibited an enhanced exhausted phenotype but possessed some functional properties during chronic HBV infection, while the Tc subset showed a lower functional level. The identification of these unique subsets may provide a potential immunotherapeutic target in chronic HBV infection. © The Author(s) 2022 |
abstractGer |
Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of $ CD8^{+} $ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry. Results There were no significant differences in the baseline characteristics between the 31 HBV carriers and the 23 sex- and age-matched HCs. $ CD8^{+} $ T cells exhibited higher levels of inhibitory receptors (TIM3 and PD1) in the HBV carriers than in the HCs (P < 0.05); in particular, Tfc cells ($ CXCR5^{+} %$ CD25^{−} $) expressed higher levels of TIM3 and PD1 than non-Tfc cells in the HBV carriers. In addition, among the subsets of Tc cells, the Tc17 ($ CXCR5^{−} %$ CD25^{−} %$ CCR6^{+} $) subset displayed increased expression of TIM3 and LAG3 in the HBV carriers. Our findings further showed that $ CD8^{+} $ T cells produced lower levels of IFN-γ, TNF-α, and Granzyme B. Paired analysis of the Tfc subset and the Tc subset indicated that higher levels of cytokines (IFN-γ and TNF-α) were produced by the Tfc subset in the HBV carriers. Among the Tc subsets, the Tc17 subset produced lower levels of cytokines. Conclusion The Tfc subset exhibited an enhanced exhausted phenotype but possessed some functional properties during chronic HBV infection, while the Tc subset showed a lower functional level. The identification of these unique subsets may provide a potential immunotherapeutic target in chronic HBV infection. © The Author(s) 2022 |
abstract_unstemmed |
Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of $ CD8^{+} $ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry. Results There were no significant differences in the baseline characteristics between the 31 HBV carriers and the 23 sex- and age-matched HCs. $ CD8^{+} $ T cells exhibited higher levels of inhibitory receptors (TIM3 and PD1) in the HBV carriers than in the HCs (P < 0.05); in particular, Tfc cells ($ CXCR5^{+} %$ CD25^{−} $) expressed higher levels of TIM3 and PD1 than non-Tfc cells in the HBV carriers. In addition, among the subsets of Tc cells, the Tc17 ($ CXCR5^{−} %$ CD25^{−} %$ CCR6^{+} $) subset displayed increased expression of TIM3 and LAG3 in the HBV carriers. Our findings further showed that $ CD8^{+} $ T cells produced lower levels of IFN-γ, TNF-α, and Granzyme B. Paired analysis of the Tfc subset and the Tc subset indicated that higher levels of cytokines (IFN-γ and TNF-α) were produced by the Tfc subset in the HBV carriers. Among the Tc subsets, the Tc17 subset produced lower levels of cytokines. Conclusion The Tfc subset exhibited an enhanced exhausted phenotype but possessed some functional properties during chronic HBV infection, while the Tc subset showed a lower functional level. The identification of these unique subsets may provide a potential immunotherapeutic target in chronic HBV infection. © The Author(s) 2022 |
collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 |
container_issue |
1 |
title_short |
Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers |
url |
https://dx.doi.org/10.1186/s12865-022-00488-2 |
remote_bool |
true |
author2 |
Chen, Can Yan, Danying Zhang, Xiaobao Liu, Xiaoxiao Yan, Dong Cui, Dawei Yang, Shigui |
author2Str |
Chen, Can Yan, Danying Zhang, Xiaobao Liu, Xiaoxiao Yan, Dong Cui, Dawei Yang, Shigui |
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doi_str |
10.1186/s12865-022-00488-2 |
up_date |
2024-07-03T16:55:16.142Z |
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