Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers

Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 he...
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Autor*in:	Jiang, Daixi [verfasserIn] Chen, Can Yan, Danying Zhang, Xiaobao Liu, Xiaoxiao Yan, Dong Cui, Dawei Yang, Shigui

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	HBV CD8 T cell CXCR5 Exhaustion Flow cytometry

Anmerkung:	© The Author(s) 2022

Übergeordnetes Werk:	Enthalten in: BMC immunology - London : BioMed Central, 2000, 23(2022), 1 vom: 20. Apr.
Übergeordnetes Werk:	volume:23 ; year:2022 ; number:1 ; day:20 ; month:04

Links:	Volltext

DOI / URN:	10.1186/s12865-022-00488-2

Katalog-ID:	SPR050653776

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245	1	0	\|a Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers
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520			\|a Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of $ CD8^{+} $ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry. Results There were no significant differences in the baseline characteristics between the 31 HBV carriers and the 23 sex- and age-matched HCs. $ CD8^{+} $ T cells exhibited higher levels of inhibitory receptors (TIM3 and PD1) in the HBV carriers than in the HCs (P < 0.05); in particular, Tfc cells ($ CXCR5^{+} %$ CD25^{−} $) expressed higher levels of TIM3 and PD1 than non-Tfc cells in the HBV carriers. In addition, among the subsets of Tc cells, the Tc17 ($ CXCR5^{−} %$ CD25^{−} %$ CCR6^{+} $) subset displayed increased expression of TIM3 and LAG3 in the HBV carriers. Our findings further showed that $ CD8^{+} $ T cells produced lower levels of IFN-γ, TNF-α, and Granzyme B. Paired analysis of the Tfc subset and the Tc subset indicated that higher levels of cytokines (IFN-γ and TNF-α) were produced by the Tfc subset in the HBV carriers. Among the Tc subsets, the Tc17 subset produced lower levels of cytokines. Conclusion The Tfc subset exhibited an enhanced exhausted phenotype but possessed some functional properties during chronic HBV infection, while the Tc subset showed a lower functional level. The identification of these unique subsets may provide a potential immunotherapeutic target in chronic HBV infection.
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700	1		\|a Yan, Dong \|4 aut
700	1		\|a Cui, Dawei \|4 aut
700	1		\|a Yang, Shigui \|4 aut
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allfields	10.1186/s12865-022-00488-2 doi (DE-627)SPR050653776 (SPR)s12865-022-00488-2-e DE-627 ger DE-627 rakwb eng Jiang, Daixi verfasserin aut Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of $ CD8^{+} $ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry. Results There were no significant differences in the baseline characteristics between the 31 HBV carriers and the 23 sex- and age-matched HCs. $ CD8^{+} $ T cells exhibited higher levels of inhibitory receptors (TIM3 and PD1) in the HBV carriers than in the HCs (P < 0.05); in particular, Tfc cells ($ CXCR5^{+} %$ CD25^{−} $) expressed higher levels of TIM3 and PD1 than non-Tfc cells in the HBV carriers. In addition, among the subsets of Tc cells, the Tc17 ($ CXCR5^{−} %$ CD25^{−} %$ CCR6^{+} $) subset displayed increased expression of TIM3 and LAG3 in the HBV carriers. Our findings further showed that $ CD8^{+} $ T cells produced lower levels of IFN-γ, TNF-α, and Granzyme B. Paired analysis of the Tfc subset and the Tc subset indicated that higher levels of cytokines (IFN-γ and TNF-α) were produced by the Tfc subset in the HBV carriers. Among the Tc subsets, the Tc17 subset produced lower levels of cytokines. Conclusion The Tfc subset exhibited an enhanced exhausted phenotype but possessed some functional properties during chronic HBV infection, while the Tc subset showed a lower functional level. The identification of these unique subsets may provide a potential immunotherapeutic target in chronic HBV infection. HBV (dpeaa)DE-He213 CD8 (dpeaa)DE-He213 T cell (dpeaa)DE-He213 CXCR5 (dpeaa)DE-He213 Exhaustion (dpeaa)DE-He213 Flow cytometry (dpeaa)DE-He213 Chen, Can aut Yan, Danying aut Zhang, Xiaobao aut Liu, Xiaoxiao aut Yan, Dong aut Cui, Dawei aut Yang, Shigui aut Enthalten in BMC immunology London : BioMed Central, 2000 23(2022), 1 vom: 20. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:23 year:2022 number:1 day:20 month:04 https://dx.doi.org/10.1186/s12865-022-00488-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 1 20 04
spelling	10.1186/s12865-022-00488-2 doi (DE-627)SPR050653776 (SPR)s12865-022-00488-2-e DE-627 ger DE-627 rakwb eng Jiang, Daixi verfasserin aut Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of $ CD8^{+} $ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry. Results There were no significant differences in the baseline characteristics between the 31 HBV carriers and the 23 sex- and age-matched HCs. $ CD8^{+} $ T cells exhibited higher levels of inhibitory receptors (TIM3 and PD1) in the HBV carriers than in the HCs (P < 0.05); in particular, Tfc cells ($ CXCR5^{+} %$ CD25^{−} $) expressed higher levels of TIM3 and PD1 than non-Tfc cells in the HBV carriers. In addition, among the subsets of Tc cells, the Tc17 ($ CXCR5^{−} %$ CD25^{−} %$ CCR6^{+} $) subset displayed increased expression of TIM3 and LAG3 in the HBV carriers. Our findings further showed that $ CD8^{+} $ T cells produced lower levels of IFN-γ, TNF-α, and Granzyme B. Paired analysis of the Tfc subset and the Tc subset indicated that higher levels of cytokines (IFN-γ and TNF-α) were produced by the Tfc subset in the HBV carriers. Among the Tc subsets, the Tc17 subset produced lower levels of cytokines. Conclusion The Tfc subset exhibited an enhanced exhausted phenotype but possessed some functional properties during chronic HBV infection, while the Tc subset showed a lower functional level. The identification of these unique subsets may provide a potential immunotherapeutic target in chronic HBV infection. HBV (dpeaa)DE-He213 CD8 (dpeaa)DE-He213 T cell (dpeaa)DE-He213 CXCR5 (dpeaa)DE-He213 Exhaustion (dpeaa)DE-He213 Flow cytometry (dpeaa)DE-He213 Chen, Can aut Yan, Danying aut Zhang, Xiaobao aut Liu, Xiaoxiao aut Yan, Dong aut Cui, Dawei aut Yang, Shigui aut Enthalten in BMC immunology London : BioMed Central, 2000 23(2022), 1 vom: 20. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:23 year:2022 number:1 day:20 month:04 https://dx.doi.org/10.1186/s12865-022-00488-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 1 20 04
allfields_unstemmed	10.1186/s12865-022-00488-2 doi (DE-627)SPR050653776 (SPR)s12865-022-00488-2-e DE-627 ger DE-627 rakwb eng Jiang, Daixi verfasserin aut Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of $ CD8^{+} $ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry. Results There were no significant differences in the baseline characteristics between the 31 HBV carriers and the 23 sex- and age-matched HCs. $ CD8^{+} $ T cells exhibited higher levels of inhibitory receptors (TIM3 and PD1) in the HBV carriers than in the HCs (P < 0.05); in particular, Tfc cells ($ CXCR5^{+} %$ CD25^{−} $) expressed higher levels of TIM3 and PD1 than non-Tfc cells in the HBV carriers. In addition, among the subsets of Tc cells, the Tc17 ($ CXCR5^{−} %$ CD25^{−} %$ CCR6^{+} $) subset displayed increased expression of TIM3 and LAG3 in the HBV carriers. Our findings further showed that $ CD8^{+} $ T cells produced lower levels of IFN-γ, TNF-α, and Granzyme B. Paired analysis of the Tfc subset and the Tc subset indicated that higher levels of cytokines (IFN-γ and TNF-α) were produced by the Tfc subset in the HBV carriers. Among the Tc subsets, the Tc17 subset produced lower levels of cytokines. Conclusion The Tfc subset exhibited an enhanced exhausted phenotype but possessed some functional properties during chronic HBV infection, while the Tc subset showed a lower functional level. The identification of these unique subsets may provide a potential immunotherapeutic target in chronic HBV infection. HBV (dpeaa)DE-He213 CD8 (dpeaa)DE-He213 T cell (dpeaa)DE-He213 CXCR5 (dpeaa)DE-He213 Exhaustion (dpeaa)DE-He213 Flow cytometry (dpeaa)DE-He213 Chen, Can aut Yan, Danying aut Zhang, Xiaobao aut Liu, Xiaoxiao aut Yan, Dong aut Cui, Dawei aut Yang, Shigui aut Enthalten in BMC immunology London : BioMed Central, 2000 23(2022), 1 vom: 20. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:23 year:2022 number:1 day:20 month:04 https://dx.doi.org/10.1186/s12865-022-00488-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 1 20 04
allfieldsGer	10.1186/s12865-022-00488-2 doi (DE-627)SPR050653776 (SPR)s12865-022-00488-2-e DE-627 ger DE-627 rakwb eng Jiang, Daixi verfasserin aut Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of $ CD8^{+} $ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry. Results There were no significant differences in the baseline characteristics between the 31 HBV carriers and the 23 sex- and age-matched HCs. $ CD8^{+} $ T cells exhibited higher levels of inhibitory receptors (TIM3 and PD1) in the HBV carriers than in the HCs (P < 0.05); in particular, Tfc cells ($ CXCR5^{+} %$ CD25^{−} $) expressed higher levels of TIM3 and PD1 than non-Tfc cells in the HBV carriers. In addition, among the subsets of Tc cells, the Tc17 ($ CXCR5^{−} %$ CD25^{−} %$ CCR6^{+} $) subset displayed increased expression of TIM3 and LAG3 in the HBV carriers. Our findings further showed that $ CD8^{+} $ T cells produced lower levels of IFN-γ, TNF-α, and Granzyme B. Paired analysis of the Tfc subset and the Tc subset indicated that higher levels of cytokines (IFN-γ and TNF-α) were produced by the Tfc subset in the HBV carriers. Among the Tc subsets, the Tc17 subset produced lower levels of cytokines. Conclusion The Tfc subset exhibited an enhanced exhausted phenotype but possessed some functional properties during chronic HBV infection, while the Tc subset showed a lower functional level. The identification of these unique subsets may provide a potential immunotherapeutic target in chronic HBV infection. HBV (dpeaa)DE-He213 CD8 (dpeaa)DE-He213 T cell (dpeaa)DE-He213 CXCR5 (dpeaa)DE-He213 Exhaustion (dpeaa)DE-He213 Flow cytometry (dpeaa)DE-He213 Chen, Can aut Yan, Danying aut Zhang, Xiaobao aut Liu, Xiaoxiao aut Yan, Dong aut Cui, Dawei aut Yang, Shigui aut Enthalten in BMC immunology London : BioMed Central, 2000 23(2022), 1 vom: 20. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:23 year:2022 number:1 day:20 month:04 https://dx.doi.org/10.1186/s12865-022-00488-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 1 20 04
allfieldsSound	10.1186/s12865-022-00488-2 doi (DE-627)SPR050653776 (SPR)s12865-022-00488-2-e DE-627 ger DE-627 rakwb eng Jiang, Daixi verfasserin aut Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of $ CD8^{+} $ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry. Results There were no significant differences in the baseline characteristics between the 31 HBV carriers and the 23 sex- and age-matched HCs. $ CD8^{+} $ T cells exhibited higher levels of inhibitory receptors (TIM3 and PD1) in the HBV carriers than in the HCs (P < 0.05); in particular, Tfc cells ($ CXCR5^{+} %$ CD25^{−} $) expressed higher levels of TIM3 and PD1 than non-Tfc cells in the HBV carriers. In addition, among the subsets of Tc cells, the Tc17 ($ CXCR5^{−} %$ CD25^{−} %$ CCR6^{+} $) subset displayed increased expression of TIM3 and LAG3 in the HBV carriers. Our findings further showed that $ CD8^{+} $ T cells produced lower levels of IFN-γ, TNF-α, and Granzyme B. Paired analysis of the Tfc subset and the Tc subset indicated that higher levels of cytokines (IFN-γ and TNF-α) were produced by the Tfc subset in the HBV carriers. Among the Tc subsets, the Tc17 subset produced lower levels of cytokines. Conclusion The Tfc subset exhibited an enhanced exhausted phenotype but possessed some functional properties during chronic HBV infection, while the Tc subset showed a lower functional level. The identification of these unique subsets may provide a potential immunotherapeutic target in chronic HBV infection. HBV (dpeaa)DE-He213 CD8 (dpeaa)DE-He213 T cell (dpeaa)DE-He213 CXCR5 (dpeaa)DE-He213 Exhaustion (dpeaa)DE-He213 Flow cytometry (dpeaa)DE-He213 Chen, Can aut Yan, Danying aut Zhang, Xiaobao aut Liu, Xiaoxiao aut Yan, Dong aut Cui, Dawei aut Yang, Shigui aut Enthalten in BMC immunology London : BioMed Central, 2000 23(2022), 1 vom: 20. Apr. (DE-627)326644962 (DE-600)2041500-X 1471-2172 nnns volume:23 year:2022 number:1 day:20 month:04 https://dx.doi.org/10.1186/s12865-022-00488-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 1 20 04
language	English
source	Enthalten in BMC immunology 23(2022), 1 vom: 20. Apr. volume:23 year:2022 number:1 day:20 month:04
sourceStr	Enthalten in BMC immunology 23(2022), 1 vom: 20. Apr. volume:23 year:2022 number:1 day:20 month:04
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	HBV CD8 T cell CXCR5 Exhaustion Flow cytometry
isfreeaccess_bool	true
container_title	BMC immunology
authorswithroles_txt_mv	Jiang, Daixi @@aut@@ Chen, Can @@aut@@ Yan, Danying @@aut@@ Zhang, Xiaobao @@aut@@ Liu, Xiaoxiao @@aut@@ Yan, Dong @@aut@@ Cui, Dawei @@aut@@ Yang, Shigui @@aut@@
publishDateDaySort_date	2022-04-20T00:00:00Z
hierarchy_top_id	326644962
id	SPR050653776
language_de	englisch
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We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of $ CD8^{+} $ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry. Results There were no significant differences in the baseline characteristics between the 31 HBV carriers and the 23 sex- and age-matched HCs. $ CD8^{+} $ T cells exhibited higher levels of inhibitory receptors (TIM3 and PD1) in the HBV carriers than in the HCs (P < 0.05); in particular, Tfc cells ($ CXCR5^{+} %$ CD25^{−} $) expressed higher levels of TIM3 and PD1 than non-Tfc cells in the HBV carriers. In addition, among the subsets of Tc cells, the Tc17 ($ CXCR5^{−} %$ CD25^{−} %$ CCR6^{+} $) subset displayed increased expression of TIM3 and LAG3 in the HBV carriers. Our findings further showed that $ CD8^{+} $ T cells produced lower levels of IFN-γ, TNF-α, and Granzyme B. Paired analysis of the Tfc subset and the Tc subset indicated that higher levels of cytokines (IFN-γ and TNF-α) were produced by the Tfc subset in the HBV carriers. Among the Tc subsets, the Tc17 subset produced lower levels of cytokines. Conclusion The Tfc subset exhibited an enhanced exhausted phenotype but possessed some functional properties during chronic HBV infection, while the Tc subset showed a lower functional level. 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author	Jiang, Daixi
spellingShingle	Jiang, Daixi misc HBV misc CD8 misc T cell misc CXCR5 misc Exhaustion misc Flow cytometry Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers
authorStr	Jiang, Daixi
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topic_title	Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers HBV (dpeaa)DE-He213 CD8 (dpeaa)DE-He213 T cell (dpeaa)DE-He213 CXCR5 (dpeaa)DE-He213 Exhaustion (dpeaa)DE-He213 Flow cytometry (dpeaa)DE-He213
topic	misc HBV misc CD8 misc T cell misc CXCR5 misc Exhaustion misc Flow cytometry
topic_unstemmed	misc HBV misc CD8 misc T cell misc CXCR5 misc Exhaustion misc Flow cytometry
topic_browse	misc HBV misc CD8 misc T cell misc CXCR5 misc Exhaustion misc Flow cytometry
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
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title	Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers
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title_full	Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers
author_sort	Jiang, Daixi
journal	BMC immunology
journalStr	BMC immunology
lang_code	eng
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author_browse	Jiang, Daixi Chen, Can Yan, Danying Zhang, Xiaobao Liu, Xiaoxiao Yan, Dong Cui, Dawei Yang, Shigui
container_volume	23
format_se	Elektronische Aufsätze
author-letter	Jiang, Daixi
doi_str_mv	10.1186/s12865-022-00488-2
title_sort	exhausted phenotype of circulating $ cd8^{+} $ t cell subsets in hepatitis b virus carriers
title_auth	Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers
abstract	Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of $ CD8^{+} $ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry. Results There were no significant differences in the baseline characteristics between the 31 HBV carriers and the 23 sex- and age-matched HCs. $ CD8^{+} $ T cells exhibited higher levels of inhibitory receptors (TIM3 and PD1) in the HBV carriers than in the HCs (P < 0.05); in particular, Tfc cells ($ CXCR5^{+} %$ CD25^{−} $) expressed higher levels of TIM3 and PD1 than non-Tfc cells in the HBV carriers. In addition, among the subsets of Tc cells, the Tc17 ($ CXCR5^{−} %$ CD25^{−} %$ CCR6^{+} $) subset displayed increased expression of TIM3 and LAG3 in the HBV carriers. Our findings further showed that $ CD8^{+} $ T cells produced lower levels of IFN-γ, TNF-α, and Granzyme B. Paired analysis of the Tfc subset and the Tc subset indicated that higher levels of cytokines (IFN-γ and TNF-α) were produced by the Tfc subset in the HBV carriers. Among the Tc subsets, the Tc17 subset produced lower levels of cytokines. Conclusion The Tfc subset exhibited an enhanced exhausted phenotype but possessed some functional properties during chronic HBV infection, while the Tc subset showed a lower functional level. The identification of these unique subsets may provide a potential immunotherapeutic target in chronic HBV infection. © The Author(s) 2022
abstractGer	Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of $ CD8^{+} $ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry. Results There were no significant differences in the baseline characteristics between the 31 HBV carriers and the 23 sex- and age-matched HCs. $ CD8^{+} $ T cells exhibited higher levels of inhibitory receptors (TIM3 and PD1) in the HBV carriers than in the HCs (P < 0.05); in particular, Tfc cells ($ CXCR5^{+} %$ CD25^{−} $) expressed higher levels of TIM3 and PD1 than non-Tfc cells in the HBV carriers. In addition, among the subsets of Tc cells, the Tc17 ($ CXCR5^{−} %$ CD25^{−} %$ CCR6^{+} $) subset displayed increased expression of TIM3 and LAG3 in the HBV carriers. Our findings further showed that $ CD8^{+} $ T cells produced lower levels of IFN-γ, TNF-α, and Granzyme B. Paired analysis of the Tfc subset and the Tc subset indicated that higher levels of cytokines (IFN-γ and TNF-α) were produced by the Tfc subset in the HBV carriers. Among the Tc subsets, the Tc17 subset produced lower levels of cytokines. Conclusion The Tfc subset exhibited an enhanced exhausted phenotype but possessed some functional properties during chronic HBV infection, while the Tc subset showed a lower functional level. The identification of these unique subsets may provide a potential immunotherapeutic target in chronic HBV infection. © The Author(s) 2022
abstract_unstemmed	Background Chronic hepatitis B virus (HBV) infection is characterized by the presence of dysfunctional exhausted $ CD8^{+} $ T cells that hamper viral control. We investigated the phenotypic heterogeneity of exhausted $ CD8^{+} $ T cells in HBV carriers. Methods We enrolled 31 HBV carriers and 23 healthy controls (HCs) in our study. Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of $ CD8^{+} $ T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based immunomicrobead assay and flow cytometry. Results There were no significant differences in the baseline characteristics between the 31 HBV carriers and the 23 sex- and age-matched HCs. $ CD8^{+} $ T cells exhibited higher levels of inhibitory receptors (TIM3 and PD1) in the HBV carriers than in the HCs (P < 0.05); in particular, Tfc cells ($ CXCR5^{+} %$ CD25^{−} $) expressed higher levels of TIM3 and PD1 than non-Tfc cells in the HBV carriers. In addition, among the subsets of Tc cells, the Tc17 ($ CXCR5^{−} %$ CD25^{−} %$ CCR6^{+} $) subset displayed increased expression of TIM3 and LAG3 in the HBV carriers. Our findings further showed that $ CD8^{+} $ T cells produced lower levels of IFN-γ, TNF-α, and Granzyme B. Paired analysis of the Tfc subset and the Tc subset indicated that higher levels of cytokines (IFN-γ and TNF-α) were produced by the Tfc subset in the HBV carriers. Among the Tc subsets, the Tc17 subset produced lower levels of cytokines. Conclusion The Tfc subset exhibited an enhanced exhausted phenotype but possessed some functional properties during chronic HBV infection, while the Tc subset showed a lower functional level. The identification of these unique subsets may provide a potential immunotherapeutic target in chronic HBV infection. © The Author(s) 2022
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container_issue	1
title_short	Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers
url	https://dx.doi.org/10.1186/s12865-022-00488-2
remote_bool	true
author2	Chen, Can Yan, Danying Zhang, Xiaobao Liu, Xiaoxiao Yan, Dong Cui, Dawei Yang, Shigui
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doi_str	10.1186/s12865-022-00488-2
up_date	2024-07-03T16:55:16.142Z
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Exhausted phenotype of circulating $ CD8^{+} $ T cell subsets in hepatitis B virus carriers

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