Development of a dual temperature control system for isoprene biosynthesis in Saccharomyces cerevisiae
Abstract Conflict between cell growth and product accumulation is frequently encountered in the biosynthesis of secondary metabolites. To address the growth-production conflict in yeast strains harboring the isoprene synthetic pathway in the mitochondria, the dynamic control of isoprene biosynthesis...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Lin, Jiaxi [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2021 |
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| Schlagwörter: |
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| Anmerkung: |
© Higher Education Press 2021 |
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| Übergeordnetes Werk: |
Enthalten in: Frontiers of chemical engineering in China - Beijing : Higher Education Press, 2007, 16(2021), 7 vom: 19. Okt., Seite 1079-1089 |
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| Übergeordnetes Werk: |
volume:16 ; year:2021 ; number:7 ; day:19 ; month:10 ; pages:1079-1089 |
| Links: |
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| DOI / URN: |
10.1007/s11705-021-2088-0 |
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SPR050868195 |
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| 520 | |a Abstract Conflict between cell growth and product accumulation is frequently encountered in the biosynthesis of secondary metabolites. To address the growth-production conflict in yeast strains harboring the isoprene synthetic pathway in the mitochondria, the dynamic control of isoprene biosynthesis was explored. A dual temperature regulation system was developed through engineering and expression regulation of the transcriptional activator Gal4p. A cold-sensitive mutant, Gal4ep19, was created by directed evolution of Gal4p based on an internally developed growth-based high-throughput screening method and expressed under the heat-shock promoter $ P_{SSA4} $ to control the expression of $ P_{GAL} $-driven pathway genes in the mitochondria. Compared to the control strain with constitutively expressed wild-type Gal4p, the dual temperature regulation strategy led to 34.5% and 72% improvements in cell growth and isoprene production, respectively. This study reports the creation of the first cold-sensitive variants of Gal4p by directed evolution and provides a dual temperature control system for yeast engineering that may also be conducive to the biosynthesis of other high-value natural products. | ||
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10.1007/s11705-021-2088-0 doi (DE-627)SPR050868195 (SPR)s11705-021-2088-0-e DE-627 ger DE-627 rakwb eng Lin, Jiaxi verfasserin aut Development of a dual temperature control system for isoprene biosynthesis in Saccharomyces cerevisiae 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Higher Education Press 2021 Abstract Conflict between cell growth and product accumulation is frequently encountered in the biosynthesis of secondary metabolites. To address the growth-production conflict in yeast strains harboring the isoprene synthetic pathway in the mitochondria, the dynamic control of isoprene biosynthesis was explored. A dual temperature regulation system was developed through engineering and expression regulation of the transcriptional activator Gal4p. A cold-sensitive mutant, Gal4ep19, was created by directed evolution of Gal4p based on an internally developed growth-based high-throughput screening method and expressed under the heat-shock promoter $ P_{SSA4} $ to control the expression of $ P_{GAL} $-driven pathway genes in the mitochondria. Compared to the control strain with constitutively expressed wild-type Gal4p, the dual temperature regulation strategy led to 34.5% and 72% improvements in cell growth and isoprene production, respectively. This study reports the creation of the first cold-sensitive variants of Gal4p by directed evolution and provides a dual temperature control system for yeast engineering that may also be conducive to the biosynthesis of other high-value natural products. transcriptional activator (dpeaa)DE-He213 directed evolution (dpeaa)DE-He213 dynamic control (dpeaa)DE-He213 heat-shock (dpeaa)DE-He213 isoprene (dpeaa)DE-He213 Yao, Zhen aut Lyu, Xiaomei aut Ye, Lidan aut Yu, Hongwei aut Enthalten in Frontiers of chemical engineering in China Beijing : Higher Education Press, 2007 16(2021), 7 vom: 19. Okt., Seite 1079-1089 (DE-627)545787602 (DE-600)2388862-3 1673-7474 nnns volume:16 year:2021 number:7 day:19 month:10 pages:1079-1089 https://dx.doi.org/10.1007/s11705-021-2088-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 AR 16 2021 7 19 10 1079-1089 |
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10.1007/s11705-021-2088-0 doi (DE-627)SPR050868195 (SPR)s11705-021-2088-0-e DE-627 ger DE-627 rakwb eng Lin, Jiaxi verfasserin aut Development of a dual temperature control system for isoprene biosynthesis in Saccharomyces cerevisiae 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Higher Education Press 2021 Abstract Conflict between cell growth and product accumulation is frequently encountered in the biosynthesis of secondary metabolites. To address the growth-production conflict in yeast strains harboring the isoprene synthetic pathway in the mitochondria, the dynamic control of isoprene biosynthesis was explored. A dual temperature regulation system was developed through engineering and expression regulation of the transcriptional activator Gal4p. A cold-sensitive mutant, Gal4ep19, was created by directed evolution of Gal4p based on an internally developed growth-based high-throughput screening method and expressed under the heat-shock promoter $ P_{SSA4} $ to control the expression of $ P_{GAL} $-driven pathway genes in the mitochondria. Compared to the control strain with constitutively expressed wild-type Gal4p, the dual temperature regulation strategy led to 34.5% and 72% improvements in cell growth and isoprene production, respectively. This study reports the creation of the first cold-sensitive variants of Gal4p by directed evolution and provides a dual temperature control system for yeast engineering that may also be conducive to the biosynthesis of other high-value natural products. transcriptional activator (dpeaa)DE-He213 directed evolution (dpeaa)DE-He213 dynamic control (dpeaa)DE-He213 heat-shock (dpeaa)DE-He213 isoprene (dpeaa)DE-He213 Yao, Zhen aut Lyu, Xiaomei aut Ye, Lidan aut Yu, Hongwei aut Enthalten in Frontiers of chemical engineering in China Beijing : Higher Education Press, 2007 16(2021), 7 vom: 19. Okt., Seite 1079-1089 (DE-627)545787602 (DE-600)2388862-3 1673-7474 nnns volume:16 year:2021 number:7 day:19 month:10 pages:1079-1089 https://dx.doi.org/10.1007/s11705-021-2088-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 AR 16 2021 7 19 10 1079-1089 |
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10.1007/s11705-021-2088-0 doi (DE-627)SPR050868195 (SPR)s11705-021-2088-0-e DE-627 ger DE-627 rakwb eng Lin, Jiaxi verfasserin aut Development of a dual temperature control system for isoprene biosynthesis in Saccharomyces cerevisiae 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Higher Education Press 2021 Abstract Conflict between cell growth and product accumulation is frequently encountered in the biosynthesis of secondary metabolites. To address the growth-production conflict in yeast strains harboring the isoprene synthetic pathway in the mitochondria, the dynamic control of isoprene biosynthesis was explored. A dual temperature regulation system was developed through engineering and expression regulation of the transcriptional activator Gal4p. A cold-sensitive mutant, Gal4ep19, was created by directed evolution of Gal4p based on an internally developed growth-based high-throughput screening method and expressed under the heat-shock promoter $ P_{SSA4} $ to control the expression of $ P_{GAL} $-driven pathway genes in the mitochondria. Compared to the control strain with constitutively expressed wild-type Gal4p, the dual temperature regulation strategy led to 34.5% and 72% improvements in cell growth and isoprene production, respectively. This study reports the creation of the first cold-sensitive variants of Gal4p by directed evolution and provides a dual temperature control system for yeast engineering that may also be conducive to the biosynthesis of other high-value natural products. transcriptional activator (dpeaa)DE-He213 directed evolution (dpeaa)DE-He213 dynamic control (dpeaa)DE-He213 heat-shock (dpeaa)DE-He213 isoprene (dpeaa)DE-He213 Yao, Zhen aut Lyu, Xiaomei aut Ye, Lidan aut Yu, Hongwei aut Enthalten in Frontiers of chemical engineering in China Beijing : Higher Education Press, 2007 16(2021), 7 vom: 19. Okt., Seite 1079-1089 (DE-627)545787602 (DE-600)2388862-3 1673-7474 nnns volume:16 year:2021 number:7 day:19 month:10 pages:1079-1089 https://dx.doi.org/10.1007/s11705-021-2088-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 AR 16 2021 7 19 10 1079-1089 |
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10.1007/s11705-021-2088-0 doi (DE-627)SPR050868195 (SPR)s11705-021-2088-0-e DE-627 ger DE-627 rakwb eng Lin, Jiaxi verfasserin aut Development of a dual temperature control system for isoprene biosynthesis in Saccharomyces cerevisiae 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Higher Education Press 2021 Abstract Conflict between cell growth and product accumulation is frequently encountered in the biosynthesis of secondary metabolites. To address the growth-production conflict in yeast strains harboring the isoprene synthetic pathway in the mitochondria, the dynamic control of isoprene biosynthesis was explored. A dual temperature regulation system was developed through engineering and expression regulation of the transcriptional activator Gal4p. A cold-sensitive mutant, Gal4ep19, was created by directed evolution of Gal4p based on an internally developed growth-based high-throughput screening method and expressed under the heat-shock promoter $ P_{SSA4} $ to control the expression of $ P_{GAL} $-driven pathway genes in the mitochondria. Compared to the control strain with constitutively expressed wild-type Gal4p, the dual temperature regulation strategy led to 34.5% and 72% improvements in cell growth and isoprene production, respectively. This study reports the creation of the first cold-sensitive variants of Gal4p by directed evolution and provides a dual temperature control system for yeast engineering that may also be conducive to the biosynthesis of other high-value natural products. transcriptional activator (dpeaa)DE-He213 directed evolution (dpeaa)DE-He213 dynamic control (dpeaa)DE-He213 heat-shock (dpeaa)DE-He213 isoprene (dpeaa)DE-He213 Yao, Zhen aut Lyu, Xiaomei aut Ye, Lidan aut Yu, Hongwei aut Enthalten in Frontiers of chemical engineering in China Beijing : Higher Education Press, 2007 16(2021), 7 vom: 19. Okt., Seite 1079-1089 (DE-627)545787602 (DE-600)2388862-3 1673-7474 nnns volume:16 year:2021 number:7 day:19 month:10 pages:1079-1089 https://dx.doi.org/10.1007/s11705-021-2088-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 AR 16 2021 7 19 10 1079-1089 |
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10.1007/s11705-021-2088-0 doi (DE-627)SPR050868195 (SPR)s11705-021-2088-0-e DE-627 ger DE-627 rakwb eng Lin, Jiaxi verfasserin aut Development of a dual temperature control system for isoprene biosynthesis in Saccharomyces cerevisiae 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Higher Education Press 2021 Abstract Conflict between cell growth and product accumulation is frequently encountered in the biosynthesis of secondary metabolites. To address the growth-production conflict in yeast strains harboring the isoprene synthetic pathway in the mitochondria, the dynamic control of isoprene biosynthesis was explored. A dual temperature regulation system was developed through engineering and expression regulation of the transcriptional activator Gal4p. A cold-sensitive mutant, Gal4ep19, was created by directed evolution of Gal4p based on an internally developed growth-based high-throughput screening method and expressed under the heat-shock promoter $ P_{SSA4} $ to control the expression of $ P_{GAL} $-driven pathway genes in the mitochondria. Compared to the control strain with constitutively expressed wild-type Gal4p, the dual temperature regulation strategy led to 34.5% and 72% improvements in cell growth and isoprene production, respectively. This study reports the creation of the first cold-sensitive variants of Gal4p by directed evolution and provides a dual temperature control system for yeast engineering that may also be conducive to the biosynthesis of other high-value natural products. transcriptional activator (dpeaa)DE-He213 directed evolution (dpeaa)DE-He213 dynamic control (dpeaa)DE-He213 heat-shock (dpeaa)DE-He213 isoprene (dpeaa)DE-He213 Yao, Zhen aut Lyu, Xiaomei aut Ye, Lidan aut Yu, Hongwei aut Enthalten in Frontiers of chemical engineering in China Beijing : Higher Education Press, 2007 16(2021), 7 vom: 19. Okt., Seite 1079-1089 (DE-627)545787602 (DE-600)2388862-3 1673-7474 nnns volume:16 year:2021 number:7 day:19 month:10 pages:1079-1089 https://dx.doi.org/10.1007/s11705-021-2088-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_152 GBV_ILN_161 GBV_ILN_171 GBV_ILN_187 GBV_ILN_224 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 AR 16 2021 7 19 10 1079-1089 |
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development of a dual temperature control system for isoprene biosynthesis in saccharomyces cerevisiae |
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Development of a dual temperature control system for isoprene biosynthesis in Saccharomyces cerevisiae |
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Abstract Conflict between cell growth and product accumulation is frequently encountered in the biosynthesis of secondary metabolites. To address the growth-production conflict in yeast strains harboring the isoprene synthetic pathway in the mitochondria, the dynamic control of isoprene biosynthesis was explored. A dual temperature regulation system was developed through engineering and expression regulation of the transcriptional activator Gal4p. A cold-sensitive mutant, Gal4ep19, was created by directed evolution of Gal4p based on an internally developed growth-based high-throughput screening method and expressed under the heat-shock promoter $ P_{SSA4} $ to control the expression of $ P_{GAL} $-driven pathway genes in the mitochondria. Compared to the control strain with constitutively expressed wild-type Gal4p, the dual temperature regulation strategy led to 34.5% and 72% improvements in cell growth and isoprene production, respectively. This study reports the creation of the first cold-sensitive variants of Gal4p by directed evolution and provides a dual temperature control system for yeast engineering that may also be conducive to the biosynthesis of other high-value natural products. © Higher Education Press 2021 |
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Abstract Conflict between cell growth and product accumulation is frequently encountered in the biosynthesis of secondary metabolites. To address the growth-production conflict in yeast strains harboring the isoprene synthetic pathway in the mitochondria, the dynamic control of isoprene biosynthesis was explored. A dual temperature regulation system was developed through engineering and expression regulation of the transcriptional activator Gal4p. A cold-sensitive mutant, Gal4ep19, was created by directed evolution of Gal4p based on an internally developed growth-based high-throughput screening method and expressed under the heat-shock promoter $ P_{SSA4} $ to control the expression of $ P_{GAL} $-driven pathway genes in the mitochondria. Compared to the control strain with constitutively expressed wild-type Gal4p, the dual temperature regulation strategy led to 34.5% and 72% improvements in cell growth and isoprene production, respectively. This study reports the creation of the first cold-sensitive variants of Gal4p by directed evolution and provides a dual temperature control system for yeast engineering that may also be conducive to the biosynthesis of other high-value natural products. © Higher Education Press 2021 |
| abstract_unstemmed |
Abstract Conflict between cell growth and product accumulation is frequently encountered in the biosynthesis of secondary metabolites. To address the growth-production conflict in yeast strains harboring the isoprene synthetic pathway in the mitochondria, the dynamic control of isoprene biosynthesis was explored. A dual temperature regulation system was developed through engineering and expression regulation of the transcriptional activator Gal4p. A cold-sensitive mutant, Gal4ep19, was created by directed evolution of Gal4p based on an internally developed growth-based high-throughput screening method and expressed under the heat-shock promoter $ P_{SSA4} $ to control the expression of $ P_{GAL} $-driven pathway genes in the mitochondria. Compared to the control strain with constitutively expressed wild-type Gal4p, the dual temperature regulation strategy led to 34.5% and 72% improvements in cell growth and isoprene production, respectively. This study reports the creation of the first cold-sensitive variants of Gal4p by directed evolution and provides a dual temperature control system for yeast engineering that may also be conducive to the biosynthesis of other high-value natural products. © Higher Education Press 2021 |
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