The effect of DC electric field on the elongation growth, proton extrusion and membrane potential of Zea mays L. coleoptile cells; a laboratory study

Background In this study, we investigated the effect of an electric field, with an intensity similar to that of the Earth’s field, on plant cells growth. The molecular mechanism underlying this effect remains unclear. Results It was found that the electric field, depending on the applied voltage, it...
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Autor*in:	Karcz, Waldemar [verfasserIn] Burdach, Zbigniew

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	L. Coleoptile segments Electric field Elongation growth Gravitropic response External medium pH Membrane potential

Anmerkung:	© The Author(s) 2022

Übergeordnetes Werk:	Enthalten in: BMC plant biology - London : BioMed Central, 2001, 22(2022), 1 vom: 03. Aug.
Übergeordnetes Werk:	volume:22 ; year:2022 ; number:1 ; day:03 ; month:08

Links:	Volltext

DOI / URN:	10.1186/s12870-022-03778-4

Katalog-ID:	SPR050899333

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520			\|a Background In this study, we investigated the effect of an electric field, with an intensity similar to that of the Earth’s field, on plant cells growth. The molecular mechanism underlying this effect remains unclear. Results It was found that the electric field, depending on the applied voltage, its duration and the polarization of the maize seedlings, stimulated or inhibited the growth of the seedling organs (root, mesocotyl and coleoptile). Moreover, it was also noticed that the gravitropic response of maize seedlings was inhibited at all voltages studied. Simultaneous measurements of growth and external medium pH show that auxin(IAA, indole-3-acetic acid)- and fusicoccin(FC)-induced elongation growth and proton extrusion of maize coleoptile segments were significantly inhibited at higher voltages. The ionic current flowing through the single coleoptile segment during voltage application was 1.7-fold lower in segments treated with cation channel blocker tetraethylammonium chloride (TEA-Cl) and 1.4-fold higher with IAA compared to the control. The electrophysiological experiments show that the electric field caused the depolarization of the membrane potential of parenchymal coleoptile cells, which was not reversible over 120 min. Conclusion It is suggested that a DC electric field inhibits the plasma membrane $ H^{+} $ pump activity and $ K^{+} $ uptake through voltage-dependent, inwardly rectifying ZMK1 channels (Zea mays $ K^{+} $ channel 1). The data presented here are discussed, taking into account the “acid growth hypothesis” of the auxin action and the mechanism of gravitropic response induction.
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allfields	10.1186/s12870-022-03778-4 doi (DE-627)SPR050899333 (SPR)s12870-022-03778-4-e DE-627 ger DE-627 rakwb eng Karcz, Waldemar verfasserin aut The effect of DC electric field on the elongation growth, proton extrusion and membrane potential of Zea mays L. coleoptile cells; a laboratory study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background In this study, we investigated the effect of an electric field, with an intensity similar to that of the Earth’s field, on plant cells growth. The molecular mechanism underlying this effect remains unclear. Results It was found that the electric field, depending on the applied voltage, its duration and the polarization of the maize seedlings, stimulated or inhibited the growth of the seedling organs (root, mesocotyl and coleoptile). Moreover, it was also noticed that the gravitropic response of maize seedlings was inhibited at all voltages studied. Simultaneous measurements of growth and external medium pH show that auxin(IAA, indole-3-acetic acid)- and fusicoccin(FC)-induced elongation growth and proton extrusion of maize coleoptile segments were significantly inhibited at higher voltages. The ionic current flowing through the single coleoptile segment during voltage application was 1.7-fold lower in segments treated with cation channel blocker tetraethylammonium chloride (TEA-Cl) and 1.4-fold higher with IAA compared to the control. The electrophysiological experiments show that the electric field caused the depolarization of the membrane potential of parenchymal coleoptile cells, which was not reversible over 120 min. Conclusion It is suggested that a DC electric field inhibits the plasma membrane $ H^{+} $ pump activity and $ K^{+} $ uptake through voltage-dependent, inwardly rectifying ZMK1 channels (Zea mays $ K^{+} $ channel 1). The data presented here are discussed, taking into account the “acid growth hypothesis” of the auxin action and the mechanism of gravitropic response induction. L. (dpeaa)DE-He213 Coleoptile segments (dpeaa)DE-He213 Electric field (dpeaa)DE-He213 Elongation growth (dpeaa)DE-He213 Gravitropic response (dpeaa)DE-He213 External medium pH (dpeaa)DE-He213 Membrane potential (dpeaa)DE-He213 Burdach, Zbigniew aut Enthalten in BMC plant biology London : BioMed Central, 2001 22(2022), 1 vom: 03. Aug. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:22 year:2022 number:1 day:03 month:08 https://dx.doi.org/10.1186/s12870-022-03778-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2022 1 03 08
spelling	10.1186/s12870-022-03778-4 doi (DE-627)SPR050899333 (SPR)s12870-022-03778-4-e DE-627 ger DE-627 rakwb eng Karcz, Waldemar verfasserin aut The effect of DC electric field on the elongation growth, proton extrusion and membrane potential of Zea mays L. coleoptile cells; a laboratory study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background In this study, we investigated the effect of an electric field, with an intensity similar to that of the Earth’s field, on plant cells growth. The molecular mechanism underlying this effect remains unclear. Results It was found that the electric field, depending on the applied voltage, its duration and the polarization of the maize seedlings, stimulated or inhibited the growth of the seedling organs (root, mesocotyl and coleoptile). Moreover, it was also noticed that the gravitropic response of maize seedlings was inhibited at all voltages studied. Simultaneous measurements of growth and external medium pH show that auxin(IAA, indole-3-acetic acid)- and fusicoccin(FC)-induced elongation growth and proton extrusion of maize coleoptile segments were significantly inhibited at higher voltages. The ionic current flowing through the single coleoptile segment during voltage application was 1.7-fold lower in segments treated with cation channel blocker tetraethylammonium chloride (TEA-Cl) and 1.4-fold higher with IAA compared to the control. The electrophysiological experiments show that the electric field caused the depolarization of the membrane potential of parenchymal coleoptile cells, which was not reversible over 120 min. Conclusion It is suggested that a DC electric field inhibits the plasma membrane $ H^{+} $ pump activity and $ K^{+} $ uptake through voltage-dependent, inwardly rectifying ZMK1 channels (Zea mays $ K^{+} $ channel 1). The data presented here are discussed, taking into account the “acid growth hypothesis” of the auxin action and the mechanism of gravitropic response induction. L. (dpeaa)DE-He213 Coleoptile segments (dpeaa)DE-He213 Electric field (dpeaa)DE-He213 Elongation growth (dpeaa)DE-He213 Gravitropic response (dpeaa)DE-He213 External medium pH (dpeaa)DE-He213 Membrane potential (dpeaa)DE-He213 Burdach, Zbigniew aut Enthalten in BMC plant biology London : BioMed Central, 2001 22(2022), 1 vom: 03. Aug. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:22 year:2022 number:1 day:03 month:08 https://dx.doi.org/10.1186/s12870-022-03778-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2022 1 03 08
allfields_unstemmed	10.1186/s12870-022-03778-4 doi (DE-627)SPR050899333 (SPR)s12870-022-03778-4-e DE-627 ger DE-627 rakwb eng Karcz, Waldemar verfasserin aut The effect of DC electric field on the elongation growth, proton extrusion and membrane potential of Zea mays L. coleoptile cells; a laboratory study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background In this study, we investigated the effect of an electric field, with an intensity similar to that of the Earth’s field, on plant cells growth. The molecular mechanism underlying this effect remains unclear. Results It was found that the electric field, depending on the applied voltage, its duration and the polarization of the maize seedlings, stimulated or inhibited the growth of the seedling organs (root, mesocotyl and coleoptile). Moreover, it was also noticed that the gravitropic response of maize seedlings was inhibited at all voltages studied. Simultaneous measurements of growth and external medium pH show that auxin(IAA, indole-3-acetic acid)- and fusicoccin(FC)-induced elongation growth and proton extrusion of maize coleoptile segments were significantly inhibited at higher voltages. The ionic current flowing through the single coleoptile segment during voltage application was 1.7-fold lower in segments treated with cation channel blocker tetraethylammonium chloride (TEA-Cl) and 1.4-fold higher with IAA compared to the control. The electrophysiological experiments show that the electric field caused the depolarization of the membrane potential of parenchymal coleoptile cells, which was not reversible over 120 min. Conclusion It is suggested that a DC electric field inhibits the plasma membrane $ H^{+} $ pump activity and $ K^{+} $ uptake through voltage-dependent, inwardly rectifying ZMK1 channels (Zea mays $ K^{+} $ channel 1). The data presented here are discussed, taking into account the “acid growth hypothesis” of the auxin action and the mechanism of gravitropic response induction. L. (dpeaa)DE-He213 Coleoptile segments (dpeaa)DE-He213 Electric field (dpeaa)DE-He213 Elongation growth (dpeaa)DE-He213 Gravitropic response (dpeaa)DE-He213 External medium pH (dpeaa)DE-He213 Membrane potential (dpeaa)DE-He213 Burdach, Zbigniew aut Enthalten in BMC plant biology London : BioMed Central, 2001 22(2022), 1 vom: 03. Aug. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:22 year:2022 number:1 day:03 month:08 https://dx.doi.org/10.1186/s12870-022-03778-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2022 1 03 08
allfieldsGer	10.1186/s12870-022-03778-4 doi (DE-627)SPR050899333 (SPR)s12870-022-03778-4-e DE-627 ger DE-627 rakwb eng Karcz, Waldemar verfasserin aut The effect of DC electric field on the elongation growth, proton extrusion and membrane potential of Zea mays L. coleoptile cells; a laboratory study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background In this study, we investigated the effect of an electric field, with an intensity similar to that of the Earth’s field, on plant cells growth. The molecular mechanism underlying this effect remains unclear. Results It was found that the electric field, depending on the applied voltage, its duration and the polarization of the maize seedlings, stimulated or inhibited the growth of the seedling organs (root, mesocotyl and coleoptile). Moreover, it was also noticed that the gravitropic response of maize seedlings was inhibited at all voltages studied. Simultaneous measurements of growth and external medium pH show that auxin(IAA, indole-3-acetic acid)- and fusicoccin(FC)-induced elongation growth and proton extrusion of maize coleoptile segments were significantly inhibited at higher voltages. The ionic current flowing through the single coleoptile segment during voltage application was 1.7-fold lower in segments treated with cation channel blocker tetraethylammonium chloride (TEA-Cl) and 1.4-fold higher with IAA compared to the control. The electrophysiological experiments show that the electric field caused the depolarization of the membrane potential of parenchymal coleoptile cells, which was not reversible over 120 min. Conclusion It is suggested that a DC electric field inhibits the plasma membrane $ H^{+} $ pump activity and $ K^{+} $ uptake through voltage-dependent, inwardly rectifying ZMK1 channels (Zea mays $ K^{+} $ channel 1). The data presented here are discussed, taking into account the “acid growth hypothesis” of the auxin action and the mechanism of gravitropic response induction. L. (dpeaa)DE-He213 Coleoptile segments (dpeaa)DE-He213 Electric field (dpeaa)DE-He213 Elongation growth (dpeaa)DE-He213 Gravitropic response (dpeaa)DE-He213 External medium pH (dpeaa)DE-He213 Membrane potential (dpeaa)DE-He213 Burdach, Zbigniew aut Enthalten in BMC plant biology London : BioMed Central, 2001 22(2022), 1 vom: 03. Aug. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:22 year:2022 number:1 day:03 month:08 https://dx.doi.org/10.1186/s12870-022-03778-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2022 1 03 08
allfieldsSound	10.1186/s12870-022-03778-4 doi (DE-627)SPR050899333 (SPR)s12870-022-03778-4-e DE-627 ger DE-627 rakwb eng Karcz, Waldemar verfasserin aut The effect of DC electric field on the elongation growth, proton extrusion and membrane potential of Zea mays L. coleoptile cells; a laboratory study 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background In this study, we investigated the effect of an electric field, with an intensity similar to that of the Earth’s field, on plant cells growth. The molecular mechanism underlying this effect remains unclear. Results It was found that the electric field, depending on the applied voltage, its duration and the polarization of the maize seedlings, stimulated or inhibited the growth of the seedling organs (root, mesocotyl and coleoptile). Moreover, it was also noticed that the gravitropic response of maize seedlings was inhibited at all voltages studied. Simultaneous measurements of growth and external medium pH show that auxin(IAA, indole-3-acetic acid)- and fusicoccin(FC)-induced elongation growth and proton extrusion of maize coleoptile segments were significantly inhibited at higher voltages. The ionic current flowing through the single coleoptile segment during voltage application was 1.7-fold lower in segments treated with cation channel blocker tetraethylammonium chloride (TEA-Cl) and 1.4-fold higher with IAA compared to the control. The electrophysiological experiments show that the electric field caused the depolarization of the membrane potential of parenchymal coleoptile cells, which was not reversible over 120 min. Conclusion It is suggested that a DC electric field inhibits the plasma membrane $ H^{+} $ pump activity and $ K^{+} $ uptake through voltage-dependent, inwardly rectifying ZMK1 channels (Zea mays $ K^{+} $ channel 1). The data presented here are discussed, taking into account the “acid growth hypothesis” of the auxin action and the mechanism of gravitropic response induction. L. (dpeaa)DE-He213 Coleoptile segments (dpeaa)DE-He213 Electric field (dpeaa)DE-He213 Elongation growth (dpeaa)DE-He213 Gravitropic response (dpeaa)DE-He213 External medium pH (dpeaa)DE-He213 Membrane potential (dpeaa)DE-He213 Burdach, Zbigniew aut Enthalten in BMC plant biology London : BioMed Central, 2001 22(2022), 1 vom: 03. Aug. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:22 year:2022 number:1 day:03 month:08 https://dx.doi.org/10.1186/s12870-022-03778-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2022 1 03 08
language	English
source	Enthalten in BMC plant biology 22(2022), 1 vom: 03. Aug. volume:22 year:2022 number:1 day:03 month:08
sourceStr	Enthalten in BMC plant biology 22(2022), 1 vom: 03. Aug. volume:22 year:2022 number:1 day:03 month:08
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	L. Coleoptile segments Electric field Elongation growth Gravitropic response External medium pH Membrane potential
isfreeaccess_bool	true
container_title	BMC plant biology
authorswithroles_txt_mv	Karcz, Waldemar @@aut@@ Burdach, Zbigniew @@aut@@
publishDateDaySort_date	2022-08-03T00:00:00Z
hierarchy_top_id	335489060
id	SPR050899333
language_de	englisch
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author	Karcz, Waldemar
spellingShingle	Karcz, Waldemar misc L. misc Coleoptile segments misc Electric field misc Elongation growth misc Gravitropic response misc External medium pH misc Membrane potential The effect of DC electric field on the elongation growth, proton extrusion and membrane potential of Zea mays L. coleoptile cells; a laboratory study
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topic_title	The effect of DC electric field on the elongation growth, proton extrusion and membrane potential of Zea mays L. coleoptile cells; a laboratory study L. (dpeaa)DE-He213 Coleoptile segments (dpeaa)DE-He213 Electric field (dpeaa)DE-He213 Elongation growth (dpeaa)DE-He213 Gravitropic response (dpeaa)DE-He213 External medium pH (dpeaa)DE-He213 Membrane potential (dpeaa)DE-He213
topic	misc L. misc Coleoptile segments misc Electric field misc Elongation growth misc Gravitropic response misc External medium pH misc Membrane potential
topic_unstemmed	misc L. misc Coleoptile segments misc Electric field misc Elongation growth misc Gravitropic response misc External medium pH misc Membrane potential
topic_browse	misc L. misc Coleoptile segments misc Electric field misc Elongation growth misc Gravitropic response misc External medium pH misc Membrane potential
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
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title	The effect of DC electric field on the elongation growth, proton extrusion and membrane potential of Zea mays L. coleoptile cells; a laboratory study
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title_full	The effect of DC electric field on the elongation growth, proton extrusion and membrane potential of Zea mays L. coleoptile cells; a laboratory study
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author_browse	Karcz, Waldemar Burdach, Zbigniew
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doi_str_mv	10.1186/s12870-022-03778-4
title_sort	effect of dc electric field on the elongation growth, proton extrusion and membrane potential of zea mays l. coleoptile cells; a laboratory study
title_auth	The effect of DC electric field on the elongation growth, proton extrusion and membrane potential of Zea mays L. coleoptile cells; a laboratory study
abstract	Background In this study, we investigated the effect of an electric field, with an intensity similar to that of the Earth’s field, on plant cells growth. The molecular mechanism underlying this effect remains unclear. Results It was found that the electric field, depending on the applied voltage, its duration and the polarization of the maize seedlings, stimulated or inhibited the growth of the seedling organs (root, mesocotyl and coleoptile). Moreover, it was also noticed that the gravitropic response of maize seedlings was inhibited at all voltages studied. Simultaneous measurements of growth and external medium pH show that auxin(IAA, indole-3-acetic acid)- and fusicoccin(FC)-induced elongation growth and proton extrusion of maize coleoptile segments were significantly inhibited at higher voltages. The ionic current flowing through the single coleoptile segment during voltage application was 1.7-fold lower in segments treated with cation channel blocker tetraethylammonium chloride (TEA-Cl) and 1.4-fold higher with IAA compared to the control. The electrophysiological experiments show that the electric field caused the depolarization of the membrane potential of parenchymal coleoptile cells, which was not reversible over 120 min. Conclusion It is suggested that a DC electric field inhibits the plasma membrane $ H^{+} $ pump activity and $ K^{+} $ uptake through voltage-dependent, inwardly rectifying ZMK1 channels (Zea mays $ K^{+} $ channel 1). The data presented here are discussed, taking into account the “acid growth hypothesis” of the auxin action and the mechanism of gravitropic response induction. © The Author(s) 2022
abstractGer	Background In this study, we investigated the effect of an electric field, with an intensity similar to that of the Earth’s field, on plant cells growth. The molecular mechanism underlying this effect remains unclear. Results It was found that the electric field, depending on the applied voltage, its duration and the polarization of the maize seedlings, stimulated or inhibited the growth of the seedling organs (root, mesocotyl and coleoptile). Moreover, it was also noticed that the gravitropic response of maize seedlings was inhibited at all voltages studied. Simultaneous measurements of growth and external medium pH show that auxin(IAA, indole-3-acetic acid)- and fusicoccin(FC)-induced elongation growth and proton extrusion of maize coleoptile segments were significantly inhibited at higher voltages. The ionic current flowing through the single coleoptile segment during voltage application was 1.7-fold lower in segments treated with cation channel blocker tetraethylammonium chloride (TEA-Cl) and 1.4-fold higher with IAA compared to the control. The electrophysiological experiments show that the electric field caused the depolarization of the membrane potential of parenchymal coleoptile cells, which was not reversible over 120 min. Conclusion It is suggested that a DC electric field inhibits the plasma membrane $ H^{+} $ pump activity and $ K^{+} $ uptake through voltage-dependent, inwardly rectifying ZMK1 channels (Zea mays $ K^{+} $ channel 1). The data presented here are discussed, taking into account the “acid growth hypothesis” of the auxin action and the mechanism of gravitropic response induction. © The Author(s) 2022
abstract_unstemmed	Background In this study, we investigated the effect of an electric field, with an intensity similar to that of the Earth’s field, on plant cells growth. The molecular mechanism underlying this effect remains unclear. Results It was found that the electric field, depending on the applied voltage, its duration and the polarization of the maize seedlings, stimulated or inhibited the growth of the seedling organs (root, mesocotyl and coleoptile). Moreover, it was also noticed that the gravitropic response of maize seedlings was inhibited at all voltages studied. Simultaneous measurements of growth and external medium pH show that auxin(IAA, indole-3-acetic acid)- and fusicoccin(FC)-induced elongation growth and proton extrusion of maize coleoptile segments were significantly inhibited at higher voltages. The ionic current flowing through the single coleoptile segment during voltage application was 1.7-fold lower in segments treated with cation channel blocker tetraethylammonium chloride (TEA-Cl) and 1.4-fold higher with IAA compared to the control. The electrophysiological experiments show that the electric field caused the depolarization of the membrane potential of parenchymal coleoptile cells, which was not reversible over 120 min. Conclusion It is suggested that a DC electric field inhibits the plasma membrane $ H^{+} $ pump activity and $ K^{+} $ uptake through voltage-dependent, inwardly rectifying ZMK1 channels (Zea mays $ K^{+} $ channel 1). The data presented here are discussed, taking into account the “acid growth hypothesis” of the auxin action and the mechanism of gravitropic response induction. © The Author(s) 2022
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title_short	The effect of DC electric field on the elongation growth, proton extrusion and membrane potential of Zea mays L. coleoptile cells; a laboratory study
url	https://dx.doi.org/10.1186/s12870-022-03778-4
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The effect of DC electric field on the elongation growth, proton extrusion and membrane potential of Zea mays L. coleoptile cells; a laboratory study

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