Xylose Fermentation Was Improved by Kluyveromyces marxianus KHM89 through Up-regulation of Nicotinamide Adenine Dinucleotide ($ NAD^{+} $) Salvage Pathway
Abstract Xylose fermentation has been reported to be improved in Kluyveromyces marxianus via strain improvement by overexpressing xylose reductase (XYL1) and xylitol dehydrogenase (XYL2). This study performed directed evolution to further enhance xylose consumption in a K. marxianus mutant following...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Kwon, Deok-Ho [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022 |
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| Schlagwörter: |
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| Anmerkung: |
© The Korean Society for Biotechnology and Bioengineering and Springer 2022 |
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| Übergeordnetes Werk: |
Enthalten in: Biotechnology and bioprocess engineering - Seoul : Society, 1996, 27(2022), 4 vom: Aug., Seite 624-630 |
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| Übergeordnetes Werk: |
volume:27 ; year:2022 ; number:4 ; month:08 ; pages:624-630 |
| Links: |
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| DOI / URN: |
10.1007/s12257-022-0036-6 |
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| Katalog-ID: |
SPR050973886 |
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| 100 | 1 | |a Kwon, Deok-Ho |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Xylose Fermentation Was Improved by Kluyveromyces marxianus KHM89 through Up-regulation of Nicotinamide Adenine Dinucleotide ($ NAD^{+} $) Salvage Pathway |
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| 520 | |a Abstract Xylose fermentation has been reported to be improved in Kluyveromyces marxianus via strain improvement by overexpressing xylose reductase (XYL1) and xylitol dehydrogenase (XYL2). This study performed directed evolution to further enhance xylose consumption in a K. marxianus mutant following transcriptomic analysis to determine genes associated with enhanced characteristics. KmXYL1 and KmXYL2 genes were overexpressed in K. marxianus 17555ΔURA3 for improving xylose fermentation. By performing directed evolution, a mutant K. marxianus KHM89 showing enhanced ethanol production was isolated from xylose medium. K. marxianus KHM89 consumed 47.39 g/L of xylose and produced 22.62 g/L of xylitol and 10.59 g/L of ethanol while the parental strain consumed 25.15 g/L of xylose and produced 7.36 g/L of xylitol and 2.05 g/L of ethanol. RNA sequencing-based transcriptomic analysis showed that alcohol dehydrogenases, aldehyde dehydrogenases, and $ NAD^{+} $ salvage pathway enzymes were upregulated in K. marxianus KHM89. These results were achieved via a combinatorial approach of rational design and directed evolution. The findings of this study contribute to the improvement of xylose fermentation by K. marxianus at an industrial scale. | ||
| 650 | 4 | |a directed evolution |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a xylose consumption |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a KHM89 |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a nicotinamide adenine dinucleotide salvage pathway |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a xylitol production |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a transcriptomic analysis |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Ha, Suk-Jin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biotechnology and bioprocess engineering |d Seoul : Society, 1996 |g 27(2022), 4 vom: Aug., Seite 624-630 |w (DE-627)373321821 |w (DE-600)2125481-3 |x 1976-3816 |7 nnns |
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10.1007/s12257-022-0036-6 doi (DE-627)SPR050973886 (SPR)s12257-022-0036-6-e DE-627 ger DE-627 rakwb eng Kwon, Deok-Ho verfasserin aut Xylose Fermentation Was Improved by Kluyveromyces marxianus KHM89 through Up-regulation of Nicotinamide Adenine Dinucleotide ($ NAD^{+} $) Salvage Pathway 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Society for Biotechnology and Bioengineering and Springer 2022 Abstract Xylose fermentation has been reported to be improved in Kluyveromyces marxianus via strain improvement by overexpressing xylose reductase (XYL1) and xylitol dehydrogenase (XYL2). This study performed directed evolution to further enhance xylose consumption in a K. marxianus mutant following transcriptomic analysis to determine genes associated with enhanced characteristics. KmXYL1 and KmXYL2 genes were overexpressed in K. marxianus 17555ΔURA3 for improving xylose fermentation. By performing directed evolution, a mutant K. marxianus KHM89 showing enhanced ethanol production was isolated from xylose medium. K. marxianus KHM89 consumed 47.39 g/L of xylose and produced 22.62 g/L of xylitol and 10.59 g/L of ethanol while the parental strain consumed 25.15 g/L of xylose and produced 7.36 g/L of xylitol and 2.05 g/L of ethanol. RNA sequencing-based transcriptomic analysis showed that alcohol dehydrogenases, aldehyde dehydrogenases, and $ NAD^{+} $ salvage pathway enzymes were upregulated in K. marxianus KHM89. These results were achieved via a combinatorial approach of rational design and directed evolution. The findings of this study contribute to the improvement of xylose fermentation by K. marxianus at an industrial scale. directed evolution (dpeaa)DE-He213 xylose consumption (dpeaa)DE-He213 KHM89 (dpeaa)DE-He213 nicotinamide adenine dinucleotide salvage pathway (dpeaa)DE-He213 xylitol production (dpeaa)DE-He213 transcriptomic analysis (dpeaa)DE-He213 Ha, Suk-Jin aut Enthalten in Biotechnology and bioprocess engineering Seoul : Society, 1996 27(2022), 4 vom: Aug., Seite 624-630 (DE-627)373321821 (DE-600)2125481-3 1976-3816 nnns volume:27 year:2022 number:4 month:08 pages:624-630 https://dx.doi.org/10.1007/s12257-022-0036-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 27 2022 4 08 624-630 |
| spelling |
10.1007/s12257-022-0036-6 doi (DE-627)SPR050973886 (SPR)s12257-022-0036-6-e DE-627 ger DE-627 rakwb eng Kwon, Deok-Ho verfasserin aut Xylose Fermentation Was Improved by Kluyveromyces marxianus KHM89 through Up-regulation of Nicotinamide Adenine Dinucleotide ($ NAD^{+} $) Salvage Pathway 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Society for Biotechnology and Bioengineering and Springer 2022 Abstract Xylose fermentation has been reported to be improved in Kluyveromyces marxianus via strain improvement by overexpressing xylose reductase (XYL1) and xylitol dehydrogenase (XYL2). This study performed directed evolution to further enhance xylose consumption in a K. marxianus mutant following transcriptomic analysis to determine genes associated with enhanced characteristics. KmXYL1 and KmXYL2 genes were overexpressed in K. marxianus 17555ΔURA3 for improving xylose fermentation. By performing directed evolution, a mutant K. marxianus KHM89 showing enhanced ethanol production was isolated from xylose medium. K. marxianus KHM89 consumed 47.39 g/L of xylose and produced 22.62 g/L of xylitol and 10.59 g/L of ethanol while the parental strain consumed 25.15 g/L of xylose and produced 7.36 g/L of xylitol and 2.05 g/L of ethanol. RNA sequencing-based transcriptomic analysis showed that alcohol dehydrogenases, aldehyde dehydrogenases, and $ NAD^{+} $ salvage pathway enzymes were upregulated in K. marxianus KHM89. These results were achieved via a combinatorial approach of rational design and directed evolution. The findings of this study contribute to the improvement of xylose fermentation by K. marxianus at an industrial scale. directed evolution (dpeaa)DE-He213 xylose consumption (dpeaa)DE-He213 KHM89 (dpeaa)DE-He213 nicotinamide adenine dinucleotide salvage pathway (dpeaa)DE-He213 xylitol production (dpeaa)DE-He213 transcriptomic analysis (dpeaa)DE-He213 Ha, Suk-Jin aut Enthalten in Biotechnology and bioprocess engineering Seoul : Society, 1996 27(2022), 4 vom: Aug., Seite 624-630 (DE-627)373321821 (DE-600)2125481-3 1976-3816 nnns volume:27 year:2022 number:4 month:08 pages:624-630 https://dx.doi.org/10.1007/s12257-022-0036-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 27 2022 4 08 624-630 |
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10.1007/s12257-022-0036-6 doi (DE-627)SPR050973886 (SPR)s12257-022-0036-6-e DE-627 ger DE-627 rakwb eng Kwon, Deok-Ho verfasserin aut Xylose Fermentation Was Improved by Kluyveromyces marxianus KHM89 through Up-regulation of Nicotinamide Adenine Dinucleotide ($ NAD^{+} $) Salvage Pathway 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Society for Biotechnology and Bioengineering and Springer 2022 Abstract Xylose fermentation has been reported to be improved in Kluyveromyces marxianus via strain improvement by overexpressing xylose reductase (XYL1) and xylitol dehydrogenase (XYL2). This study performed directed evolution to further enhance xylose consumption in a K. marxianus mutant following transcriptomic analysis to determine genes associated with enhanced characteristics. KmXYL1 and KmXYL2 genes were overexpressed in K. marxianus 17555ΔURA3 for improving xylose fermentation. By performing directed evolution, a mutant K. marxianus KHM89 showing enhanced ethanol production was isolated from xylose medium. K. marxianus KHM89 consumed 47.39 g/L of xylose and produced 22.62 g/L of xylitol and 10.59 g/L of ethanol while the parental strain consumed 25.15 g/L of xylose and produced 7.36 g/L of xylitol and 2.05 g/L of ethanol. RNA sequencing-based transcriptomic analysis showed that alcohol dehydrogenases, aldehyde dehydrogenases, and $ NAD^{+} $ salvage pathway enzymes were upregulated in K. marxianus KHM89. These results were achieved via a combinatorial approach of rational design and directed evolution. The findings of this study contribute to the improvement of xylose fermentation by K. marxianus at an industrial scale. directed evolution (dpeaa)DE-He213 xylose consumption (dpeaa)DE-He213 KHM89 (dpeaa)DE-He213 nicotinamide adenine dinucleotide salvage pathway (dpeaa)DE-He213 xylitol production (dpeaa)DE-He213 transcriptomic analysis (dpeaa)DE-He213 Ha, Suk-Jin aut Enthalten in Biotechnology and bioprocess engineering Seoul : Society, 1996 27(2022), 4 vom: Aug., Seite 624-630 (DE-627)373321821 (DE-600)2125481-3 1976-3816 nnns volume:27 year:2022 number:4 month:08 pages:624-630 https://dx.doi.org/10.1007/s12257-022-0036-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 27 2022 4 08 624-630 |
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10.1007/s12257-022-0036-6 doi (DE-627)SPR050973886 (SPR)s12257-022-0036-6-e DE-627 ger DE-627 rakwb eng Kwon, Deok-Ho verfasserin aut Xylose Fermentation Was Improved by Kluyveromyces marxianus KHM89 through Up-regulation of Nicotinamide Adenine Dinucleotide ($ NAD^{+} $) Salvage Pathway 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Society for Biotechnology and Bioengineering and Springer 2022 Abstract Xylose fermentation has been reported to be improved in Kluyveromyces marxianus via strain improvement by overexpressing xylose reductase (XYL1) and xylitol dehydrogenase (XYL2). This study performed directed evolution to further enhance xylose consumption in a K. marxianus mutant following transcriptomic analysis to determine genes associated with enhanced characteristics. KmXYL1 and KmXYL2 genes were overexpressed in K. marxianus 17555ΔURA3 for improving xylose fermentation. By performing directed evolution, a mutant K. marxianus KHM89 showing enhanced ethanol production was isolated from xylose medium. K. marxianus KHM89 consumed 47.39 g/L of xylose and produced 22.62 g/L of xylitol and 10.59 g/L of ethanol while the parental strain consumed 25.15 g/L of xylose and produced 7.36 g/L of xylitol and 2.05 g/L of ethanol. RNA sequencing-based transcriptomic analysis showed that alcohol dehydrogenases, aldehyde dehydrogenases, and $ NAD^{+} $ salvage pathway enzymes were upregulated in K. marxianus KHM89. These results were achieved via a combinatorial approach of rational design and directed evolution. The findings of this study contribute to the improvement of xylose fermentation by K. marxianus at an industrial scale. directed evolution (dpeaa)DE-He213 xylose consumption (dpeaa)DE-He213 KHM89 (dpeaa)DE-He213 nicotinamide adenine dinucleotide salvage pathway (dpeaa)DE-He213 xylitol production (dpeaa)DE-He213 transcriptomic analysis (dpeaa)DE-He213 Ha, Suk-Jin aut Enthalten in Biotechnology and bioprocess engineering Seoul : Society, 1996 27(2022), 4 vom: Aug., Seite 624-630 (DE-627)373321821 (DE-600)2125481-3 1976-3816 nnns volume:27 year:2022 number:4 month:08 pages:624-630 https://dx.doi.org/10.1007/s12257-022-0036-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 27 2022 4 08 624-630 |
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10.1007/s12257-022-0036-6 doi (DE-627)SPR050973886 (SPR)s12257-022-0036-6-e DE-627 ger DE-627 rakwb eng Kwon, Deok-Ho verfasserin aut Xylose Fermentation Was Improved by Kluyveromyces marxianus KHM89 through Up-regulation of Nicotinamide Adenine Dinucleotide ($ NAD^{+} $) Salvage Pathway 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Society for Biotechnology and Bioengineering and Springer 2022 Abstract Xylose fermentation has been reported to be improved in Kluyveromyces marxianus via strain improvement by overexpressing xylose reductase (XYL1) and xylitol dehydrogenase (XYL2). This study performed directed evolution to further enhance xylose consumption in a K. marxianus mutant following transcriptomic analysis to determine genes associated with enhanced characteristics. KmXYL1 and KmXYL2 genes were overexpressed in K. marxianus 17555ΔURA3 for improving xylose fermentation. By performing directed evolution, a mutant K. marxianus KHM89 showing enhanced ethanol production was isolated from xylose medium. K. marxianus KHM89 consumed 47.39 g/L of xylose and produced 22.62 g/L of xylitol and 10.59 g/L of ethanol while the parental strain consumed 25.15 g/L of xylose and produced 7.36 g/L of xylitol and 2.05 g/L of ethanol. RNA sequencing-based transcriptomic analysis showed that alcohol dehydrogenases, aldehyde dehydrogenases, and $ NAD^{+} $ salvage pathway enzymes were upregulated in K. marxianus KHM89. These results were achieved via a combinatorial approach of rational design and directed evolution. The findings of this study contribute to the improvement of xylose fermentation by K. marxianus at an industrial scale. directed evolution (dpeaa)DE-He213 xylose consumption (dpeaa)DE-He213 KHM89 (dpeaa)DE-He213 nicotinamide adenine dinucleotide salvage pathway (dpeaa)DE-He213 xylitol production (dpeaa)DE-He213 transcriptomic analysis (dpeaa)DE-He213 Ha, Suk-Jin aut Enthalten in Biotechnology and bioprocess engineering Seoul : Society, 1996 27(2022), 4 vom: Aug., Seite 624-630 (DE-627)373321821 (DE-600)2125481-3 1976-3816 nnns volume:27 year:2022 number:4 month:08 pages:624-630 https://dx.doi.org/10.1007/s12257-022-0036-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 27 2022 4 08 624-630 |
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Enthalten in Biotechnology and bioprocess engineering 27(2022), 4 vom: Aug., Seite 624-630 volume:27 year:2022 number:4 month:08 pages:624-630 |
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Enthalten in Biotechnology and bioprocess engineering 27(2022), 4 vom: Aug., Seite 624-630 volume:27 year:2022 number:4 month:08 pages:624-630 |
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directed evolution xylose consumption KHM89 nicotinamide adenine dinucleotide salvage pathway xylitol production transcriptomic analysis |
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Kwon, Deok-Ho @@aut@@ Ha, Suk-Jin @@aut@@ |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">SPR050973886</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230508035953.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230508s2022 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s12257-022-0036-6</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR050973886</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s12257-022-0036-6-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Kwon, Deok-Ho</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Xylose Fermentation Was Improved by Kluyveromyces marxianus KHM89 through Up-regulation of Nicotinamide Adenine Dinucleotide ($ NAD^{+} $) Salvage Pathway</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2022</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© The Korean Society for Biotechnology and Bioengineering and Springer 2022</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Xylose fermentation has been reported to be improved in Kluyveromyces marxianus via strain improvement by overexpressing xylose reductase (XYL1) and xylitol dehydrogenase (XYL2). This study performed directed evolution to further enhance xylose consumption in a K. marxianus mutant following transcriptomic analysis to determine genes associated with enhanced characteristics. KmXYL1 and KmXYL2 genes were overexpressed in K. marxianus 17555ΔURA3 for improving xylose fermentation. By performing directed evolution, a mutant K. marxianus KHM89 showing enhanced ethanol production was isolated from xylose medium. K. marxianus KHM89 consumed 47.39 g/L of xylose and produced 22.62 g/L of xylitol and 10.59 g/L of ethanol while the parental strain consumed 25.15 g/L of xylose and produced 7.36 g/L of xylitol and 2.05 g/L of ethanol. RNA sequencing-based transcriptomic analysis showed that alcohol dehydrogenases, aldehyde dehydrogenases, and $ NAD^{+} $ salvage pathway enzymes were upregulated in K. marxianus KHM89. These results were achieved via a combinatorial approach of rational design and directed evolution. 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Kwon, Deok-Ho |
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Kwon, Deok-Ho misc directed evolution misc xylose consumption misc KHM89 misc nicotinamide adenine dinucleotide salvage pathway misc xylitol production misc transcriptomic analysis Xylose Fermentation Was Improved by Kluyveromyces marxianus KHM89 through Up-regulation of Nicotinamide Adenine Dinucleotide ($ NAD^{+} $) Salvage Pathway |
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Xylose Fermentation Was Improved by Kluyveromyces marxianus KHM89 through Up-regulation of Nicotinamide Adenine Dinucleotide ($ NAD^{+} $) Salvage Pathway directed evolution (dpeaa)DE-He213 xylose consumption (dpeaa)DE-He213 KHM89 (dpeaa)DE-He213 nicotinamide adenine dinucleotide salvage pathway (dpeaa)DE-He213 xylitol production (dpeaa)DE-He213 transcriptomic analysis (dpeaa)DE-He213 |
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misc directed evolution misc xylose consumption misc KHM89 misc nicotinamide adenine dinucleotide salvage pathway misc xylitol production misc transcriptomic analysis |
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Xylose Fermentation Was Improved by Kluyveromyces marxianus KHM89 through Up-regulation of Nicotinamide Adenine Dinucleotide ($ NAD^{+} $) Salvage Pathway |
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Xylose Fermentation Was Improved by Kluyveromyces marxianus KHM89 through Up-regulation of Nicotinamide Adenine Dinucleotide ($ NAD^{+} $) Salvage Pathway |
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Kwon, Deok-Ho |
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xylose fermentation was improved by kluyveromyces marxianus khm89 through up-regulation of nicotinamide adenine dinucleotide ($ nad^{+} $) salvage pathway |
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Xylose Fermentation Was Improved by Kluyveromyces marxianus KHM89 through Up-regulation of Nicotinamide Adenine Dinucleotide ($ NAD^{+} $) Salvage Pathway |
| abstract |
Abstract Xylose fermentation has been reported to be improved in Kluyveromyces marxianus via strain improvement by overexpressing xylose reductase (XYL1) and xylitol dehydrogenase (XYL2). This study performed directed evolution to further enhance xylose consumption in a K. marxianus mutant following transcriptomic analysis to determine genes associated with enhanced characteristics. KmXYL1 and KmXYL2 genes were overexpressed in K. marxianus 17555ΔURA3 for improving xylose fermentation. By performing directed evolution, a mutant K. marxianus KHM89 showing enhanced ethanol production was isolated from xylose medium. K. marxianus KHM89 consumed 47.39 g/L of xylose and produced 22.62 g/L of xylitol and 10.59 g/L of ethanol while the parental strain consumed 25.15 g/L of xylose and produced 7.36 g/L of xylitol and 2.05 g/L of ethanol. RNA sequencing-based transcriptomic analysis showed that alcohol dehydrogenases, aldehyde dehydrogenases, and $ NAD^{+} $ salvage pathway enzymes were upregulated in K. marxianus KHM89. These results were achieved via a combinatorial approach of rational design and directed evolution. The findings of this study contribute to the improvement of xylose fermentation by K. marxianus at an industrial scale. © The Korean Society for Biotechnology and Bioengineering and Springer 2022 |
| abstractGer |
Abstract Xylose fermentation has been reported to be improved in Kluyveromyces marxianus via strain improvement by overexpressing xylose reductase (XYL1) and xylitol dehydrogenase (XYL2). This study performed directed evolution to further enhance xylose consumption in a K. marxianus mutant following transcriptomic analysis to determine genes associated with enhanced characteristics. KmXYL1 and KmXYL2 genes were overexpressed in K. marxianus 17555ΔURA3 for improving xylose fermentation. By performing directed evolution, a mutant K. marxianus KHM89 showing enhanced ethanol production was isolated from xylose medium. K. marxianus KHM89 consumed 47.39 g/L of xylose and produced 22.62 g/L of xylitol and 10.59 g/L of ethanol while the parental strain consumed 25.15 g/L of xylose and produced 7.36 g/L of xylitol and 2.05 g/L of ethanol. RNA sequencing-based transcriptomic analysis showed that alcohol dehydrogenases, aldehyde dehydrogenases, and $ NAD^{+} $ salvage pathway enzymes were upregulated in K. marxianus KHM89. These results were achieved via a combinatorial approach of rational design and directed evolution. The findings of this study contribute to the improvement of xylose fermentation by K. marxianus at an industrial scale. © The Korean Society for Biotechnology and Bioengineering and Springer 2022 |
| abstract_unstemmed |
Abstract Xylose fermentation has been reported to be improved in Kluyveromyces marxianus via strain improvement by overexpressing xylose reductase (XYL1) and xylitol dehydrogenase (XYL2). This study performed directed evolution to further enhance xylose consumption in a K. marxianus mutant following transcriptomic analysis to determine genes associated with enhanced characteristics. KmXYL1 and KmXYL2 genes were overexpressed in K. marxianus 17555ΔURA3 for improving xylose fermentation. By performing directed evolution, a mutant K. marxianus KHM89 showing enhanced ethanol production was isolated from xylose medium. K. marxianus KHM89 consumed 47.39 g/L of xylose and produced 22.62 g/L of xylitol and 10.59 g/L of ethanol while the parental strain consumed 25.15 g/L of xylose and produced 7.36 g/L of xylitol and 2.05 g/L of ethanol. RNA sequencing-based transcriptomic analysis showed that alcohol dehydrogenases, aldehyde dehydrogenases, and $ NAD^{+} $ salvage pathway enzymes were upregulated in K. marxianus KHM89. These results were achieved via a combinatorial approach of rational design and directed evolution. The findings of this study contribute to the improvement of xylose fermentation by K. marxianus at an industrial scale. © The Korean Society for Biotechnology and Bioengineering and Springer 2022 |
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4 |
| title_short |
Xylose Fermentation Was Improved by Kluyveromyces marxianus KHM89 through Up-regulation of Nicotinamide Adenine Dinucleotide ($ NAD^{+} $) Salvage Pathway |
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https://dx.doi.org/10.1007/s12257-022-0036-6 |
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Ha, Suk-Jin |
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10.1007/s12257-022-0036-6 |
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2024-07-03T18:58:44.208Z |
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| score |
7.3989973 |