Surgical implantation of human adipose derived stem cells attenuates experimentally induced hepatic fibrosis in rats

Background Mesenchymal stem cells (MSCs) are multipotent stromal cells and could exert hepatoprotective effects against acute liver injury, steatohepatitis, and fibrogenesis. Here, we evaluated the effects of human adipose derived stem cells (hADSCs) to attenuate experimentally induced hepatic fibro...
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Autor*in:	Nomura, Masateru [verfasserIn] George, Joseph Hashizume, Chieko Saito, Takashi Ueda, Yoshimichi Ishigaki, Yasuhito Tsuchishima, Mutsumi Tsutsumi, Mikihiro

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Adipose derived stem cells hADSCs Mesenchymal stem cells MSCs Hepatic fibrosis Liver cirrhosis Carbon tetrachloride

Anmerkung:	© The Author(s) 2022

Übergeordnetes Werk:	Enthalten in: Molecular medicine - [London] : BioMed Central, 1994, 28(2022), 1 vom: 29. Nov.
Übergeordnetes Werk:	volume:28 ; year:2022 ; number:1 ; day:29 ; month:11

Links:	Volltext

DOI / URN:	10.1186/s10020-022-00566-6

Katalog-ID:	SPR051186772

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245	1	0	\|a Surgical implantation of human adipose derived stem cells attenuates experimentally induced hepatic fibrosis in rats
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520			\|a Background Mesenchymal stem cells (MSCs) are multipotent stromal cells and could exert hepatoprotective effects against acute liver injury, steatohepatitis, and fibrogenesis. Here, we evaluated the effects of human adipose derived stem cells (hADSCs) to attenuate experimentally induced hepatic fibrosis and early cirrhosis in rats. Methods Hepatic fibrosis was induced by intraperitoneal injections of $ CCl_{4} $ (0.1 ml/100 g body weight) twice a week for 8 weeks. hADSCs were isolated and cultured on polyethylene discs coated with hydroxyapatite and 2 cm diameter disc was surgically implanted on the right lateral lobe of the liver. Discs implanted without hADSCs served as control. The animals were injected again with $ CCl_{4} $ once a week for another 8 weeks. All the animals were sacrificed at the end of 16th week. Results Serial administrations of $ CCl_{4} $ resulted in well developed fibrosis and early cirrhosis at 8th week which maintained until the 16th week. Animals treated with hADSC discs depicted over 50% decrease of collagen with significant increase in serum albumin and total protein levels. Immunohistochemical staining for TGF-β1, α-smooth muscle actin, and collagen type I and type III demonstrated marked decrease compared to the animals without hADSC treatment. Conclusions Treatment with hADSCs improved liver functions, markedly reduced hepatic fibrosis and early cirrhosis. Various pleiotropic and paracrine factors secreted from the hADSCs seem to serve as reparative functions in the attenuation of liver cirrhosis. The data demonstrated that treatment with hADSCs can be successfully used as a potent therapeutic method to prevent progression of hepatic fibrosis and related adverse events.
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allfields	10.1186/s10020-022-00566-6 doi (DE-627)SPR051186772 (SPR)s10020-022-00566-6-e DE-627 ger DE-627 rakwb eng Nomura, Masateru verfasserin aut Surgical implantation of human adipose derived stem cells attenuates experimentally induced hepatic fibrosis in rats 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Mesenchymal stem cells (MSCs) are multipotent stromal cells and could exert hepatoprotective effects against acute liver injury, steatohepatitis, and fibrogenesis. Here, we evaluated the effects of human adipose derived stem cells (hADSCs) to attenuate experimentally induced hepatic fibrosis and early cirrhosis in rats. Methods Hepatic fibrosis was induced by intraperitoneal injections of $ CCl_{4} $ (0.1 ml/100 g body weight) twice a week for 8 weeks. hADSCs were isolated and cultured on polyethylene discs coated with hydroxyapatite and 2 cm diameter disc was surgically implanted on the right lateral lobe of the liver. Discs implanted without hADSCs served as control. The animals were injected again with $ CCl_{4} $ once a week for another 8 weeks. All the animals were sacrificed at the end of 16th week. Results Serial administrations of $ CCl_{4} $ resulted in well developed fibrosis and early cirrhosis at 8th week which maintained until the 16th week. Animals treated with hADSC discs depicted over 50% decrease of collagen with significant increase in serum albumin and total protein levels. Immunohistochemical staining for TGF-β1, α-smooth muscle actin, and collagen type I and type III demonstrated marked decrease compared to the animals without hADSC treatment. Conclusions Treatment with hADSCs improved liver functions, markedly reduced hepatic fibrosis and early cirrhosis. Various pleiotropic and paracrine factors secreted from the hADSCs seem to serve as reparative functions in the attenuation of liver cirrhosis. The data demonstrated that treatment with hADSCs can be successfully used as a potent therapeutic method to prevent progression of hepatic fibrosis and related adverse events. Adipose derived stem cells (dpeaa)DE-He213 hADSCs (dpeaa)DE-He213 Mesenchymal stem cells (dpeaa)DE-He213 MSCs (dpeaa)DE-He213 Hepatic fibrosis (dpeaa)DE-He213 Liver cirrhosis (dpeaa)DE-He213 Carbon tetrachloride (dpeaa)DE-He213 George, Joseph (orcid)0000-0001-5354-7884 aut Hashizume, Chieko aut Saito, Takashi aut Ueda, Yoshimichi aut Ishigaki, Yasuhito aut Tsuchishima, Mutsumi aut Tsutsumi, Mikihiro (orcid)0000-0003-0463-074X aut Enthalten in Molecular medicine [London] : BioMed Central, 1994 28(2022), 1 vom: 29. Nov. (DE-627)269539611 (DE-600)1475577-4 1528-3658 nnns volume:28 year:2022 number:1 day:29 month:11 https://dx.doi.org/10.1186/s10020-022-00566-6 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2153 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2022 1 29 11
spelling	10.1186/s10020-022-00566-6 doi (DE-627)SPR051186772 (SPR)s10020-022-00566-6-e DE-627 ger DE-627 rakwb eng Nomura, Masateru verfasserin aut Surgical implantation of human adipose derived stem cells attenuates experimentally induced hepatic fibrosis in rats 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Mesenchymal stem cells (MSCs) are multipotent stromal cells and could exert hepatoprotective effects against acute liver injury, steatohepatitis, and fibrogenesis. Here, we evaluated the effects of human adipose derived stem cells (hADSCs) to attenuate experimentally induced hepatic fibrosis and early cirrhosis in rats. Methods Hepatic fibrosis was induced by intraperitoneal injections of $ CCl_{4} $ (0.1 ml/100 g body weight) twice a week for 8 weeks. hADSCs were isolated and cultured on polyethylene discs coated with hydroxyapatite and 2 cm diameter disc was surgically implanted on the right lateral lobe of the liver. Discs implanted without hADSCs served as control. The animals were injected again with $ CCl_{4} $ once a week for another 8 weeks. All the animals were sacrificed at the end of 16th week. Results Serial administrations of $ CCl_{4} $ resulted in well developed fibrosis and early cirrhosis at 8th week which maintained until the 16th week. Animals treated with hADSC discs depicted over 50% decrease of collagen with significant increase in serum albumin and total protein levels. Immunohistochemical staining for TGF-β1, α-smooth muscle actin, and collagen type I and type III demonstrated marked decrease compared to the animals without hADSC treatment. Conclusions Treatment with hADSCs improved liver functions, markedly reduced hepatic fibrosis and early cirrhosis. Various pleiotropic and paracrine factors secreted from the hADSCs seem to serve as reparative functions in the attenuation of liver cirrhosis. The data demonstrated that treatment with hADSCs can be successfully used as a potent therapeutic method to prevent progression of hepatic fibrosis and related adverse events. Adipose derived stem cells (dpeaa)DE-He213 hADSCs (dpeaa)DE-He213 Mesenchymal stem cells (dpeaa)DE-He213 MSCs (dpeaa)DE-He213 Hepatic fibrosis (dpeaa)DE-He213 Liver cirrhosis (dpeaa)DE-He213 Carbon tetrachloride (dpeaa)DE-He213 George, Joseph (orcid)0000-0001-5354-7884 aut Hashizume, Chieko aut Saito, Takashi aut Ueda, Yoshimichi aut Ishigaki, Yasuhito aut Tsuchishima, Mutsumi aut Tsutsumi, Mikihiro (orcid)0000-0003-0463-074X aut Enthalten in Molecular medicine [London] : BioMed Central, 1994 28(2022), 1 vom: 29. Nov. (DE-627)269539611 (DE-600)1475577-4 1528-3658 nnns volume:28 year:2022 number:1 day:29 month:11 https://dx.doi.org/10.1186/s10020-022-00566-6 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2153 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2022 1 29 11
allfields_unstemmed	10.1186/s10020-022-00566-6 doi (DE-627)SPR051186772 (SPR)s10020-022-00566-6-e DE-627 ger DE-627 rakwb eng Nomura, Masateru verfasserin aut Surgical implantation of human adipose derived stem cells attenuates experimentally induced hepatic fibrosis in rats 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Mesenchymal stem cells (MSCs) are multipotent stromal cells and could exert hepatoprotective effects against acute liver injury, steatohepatitis, and fibrogenesis. Here, we evaluated the effects of human adipose derived stem cells (hADSCs) to attenuate experimentally induced hepatic fibrosis and early cirrhosis in rats. Methods Hepatic fibrosis was induced by intraperitoneal injections of $ CCl_{4} $ (0.1 ml/100 g body weight) twice a week for 8 weeks. hADSCs were isolated and cultured on polyethylene discs coated with hydroxyapatite and 2 cm diameter disc was surgically implanted on the right lateral lobe of the liver. Discs implanted without hADSCs served as control. The animals were injected again with $ CCl_{4} $ once a week for another 8 weeks. All the animals were sacrificed at the end of 16th week. Results Serial administrations of $ CCl_{4} $ resulted in well developed fibrosis and early cirrhosis at 8th week which maintained until the 16th week. Animals treated with hADSC discs depicted over 50% decrease of collagen with significant increase in serum albumin and total protein levels. Immunohistochemical staining for TGF-β1, α-smooth muscle actin, and collagen type I and type III demonstrated marked decrease compared to the animals without hADSC treatment. Conclusions Treatment with hADSCs improved liver functions, markedly reduced hepatic fibrosis and early cirrhosis. Various pleiotropic and paracrine factors secreted from the hADSCs seem to serve as reparative functions in the attenuation of liver cirrhosis. The data demonstrated that treatment with hADSCs can be successfully used as a potent therapeutic method to prevent progression of hepatic fibrosis and related adverse events. Adipose derived stem cells (dpeaa)DE-He213 hADSCs (dpeaa)DE-He213 Mesenchymal stem cells (dpeaa)DE-He213 MSCs (dpeaa)DE-He213 Hepatic fibrosis (dpeaa)DE-He213 Liver cirrhosis (dpeaa)DE-He213 Carbon tetrachloride (dpeaa)DE-He213 George, Joseph (orcid)0000-0001-5354-7884 aut Hashizume, Chieko aut Saito, Takashi aut Ueda, Yoshimichi aut Ishigaki, Yasuhito aut Tsuchishima, Mutsumi aut Tsutsumi, Mikihiro (orcid)0000-0003-0463-074X aut Enthalten in Molecular medicine [London] : BioMed Central, 1994 28(2022), 1 vom: 29. Nov. (DE-627)269539611 (DE-600)1475577-4 1528-3658 nnns volume:28 year:2022 number:1 day:29 month:11 https://dx.doi.org/10.1186/s10020-022-00566-6 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2153 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2022 1 29 11
allfieldsGer	10.1186/s10020-022-00566-6 doi (DE-627)SPR051186772 (SPR)s10020-022-00566-6-e DE-627 ger DE-627 rakwb eng Nomura, Masateru verfasserin aut Surgical implantation of human adipose derived stem cells attenuates experimentally induced hepatic fibrosis in rats 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Mesenchymal stem cells (MSCs) are multipotent stromal cells and could exert hepatoprotective effects against acute liver injury, steatohepatitis, and fibrogenesis. Here, we evaluated the effects of human adipose derived stem cells (hADSCs) to attenuate experimentally induced hepatic fibrosis and early cirrhosis in rats. Methods Hepatic fibrosis was induced by intraperitoneal injections of $ CCl_{4} $ (0.1 ml/100 g body weight) twice a week for 8 weeks. hADSCs were isolated and cultured on polyethylene discs coated with hydroxyapatite and 2 cm diameter disc was surgically implanted on the right lateral lobe of the liver. Discs implanted without hADSCs served as control. The animals were injected again with $ CCl_{4} $ once a week for another 8 weeks. All the animals were sacrificed at the end of 16th week. Results Serial administrations of $ CCl_{4} $ resulted in well developed fibrosis and early cirrhosis at 8th week which maintained until the 16th week. Animals treated with hADSC discs depicted over 50% decrease of collagen with significant increase in serum albumin and total protein levels. Immunohistochemical staining for TGF-β1, α-smooth muscle actin, and collagen type I and type III demonstrated marked decrease compared to the animals without hADSC treatment. Conclusions Treatment with hADSCs improved liver functions, markedly reduced hepatic fibrosis and early cirrhosis. Various pleiotropic and paracrine factors secreted from the hADSCs seem to serve as reparative functions in the attenuation of liver cirrhosis. The data demonstrated that treatment with hADSCs can be successfully used as a potent therapeutic method to prevent progression of hepatic fibrosis and related adverse events. Adipose derived stem cells (dpeaa)DE-He213 hADSCs (dpeaa)DE-He213 Mesenchymal stem cells (dpeaa)DE-He213 MSCs (dpeaa)DE-He213 Hepatic fibrosis (dpeaa)DE-He213 Liver cirrhosis (dpeaa)DE-He213 Carbon tetrachloride (dpeaa)DE-He213 George, Joseph (orcid)0000-0001-5354-7884 aut Hashizume, Chieko aut Saito, Takashi aut Ueda, Yoshimichi aut Ishigaki, Yasuhito aut Tsuchishima, Mutsumi aut Tsutsumi, Mikihiro (orcid)0000-0003-0463-074X aut Enthalten in Molecular medicine [London] : BioMed Central, 1994 28(2022), 1 vom: 29. Nov. (DE-627)269539611 (DE-600)1475577-4 1528-3658 nnns volume:28 year:2022 number:1 day:29 month:11 https://dx.doi.org/10.1186/s10020-022-00566-6 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2153 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2022 1 29 11
allfieldsSound	10.1186/s10020-022-00566-6 doi (DE-627)SPR051186772 (SPR)s10020-022-00566-6-e DE-627 ger DE-627 rakwb eng Nomura, Masateru verfasserin aut Surgical implantation of human adipose derived stem cells attenuates experimentally induced hepatic fibrosis in rats 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Background Mesenchymal stem cells (MSCs) are multipotent stromal cells and could exert hepatoprotective effects against acute liver injury, steatohepatitis, and fibrogenesis. Here, we evaluated the effects of human adipose derived stem cells (hADSCs) to attenuate experimentally induced hepatic fibrosis and early cirrhosis in rats. Methods Hepatic fibrosis was induced by intraperitoneal injections of $ CCl_{4} $ (0.1 ml/100 g body weight) twice a week for 8 weeks. hADSCs were isolated and cultured on polyethylene discs coated with hydroxyapatite and 2 cm diameter disc was surgically implanted on the right lateral lobe of the liver. Discs implanted without hADSCs served as control. The animals were injected again with $ CCl_{4} $ once a week for another 8 weeks. All the animals were sacrificed at the end of 16th week. Results Serial administrations of $ CCl_{4} $ resulted in well developed fibrosis and early cirrhosis at 8th week which maintained until the 16th week. Animals treated with hADSC discs depicted over 50% decrease of collagen with significant increase in serum albumin and total protein levels. Immunohistochemical staining for TGF-β1, α-smooth muscle actin, and collagen type I and type III demonstrated marked decrease compared to the animals without hADSC treatment. Conclusions Treatment with hADSCs improved liver functions, markedly reduced hepatic fibrosis and early cirrhosis. Various pleiotropic and paracrine factors secreted from the hADSCs seem to serve as reparative functions in the attenuation of liver cirrhosis. The data demonstrated that treatment with hADSCs can be successfully used as a potent therapeutic method to prevent progression of hepatic fibrosis and related adverse events. Adipose derived stem cells (dpeaa)DE-He213 hADSCs (dpeaa)DE-He213 Mesenchymal stem cells (dpeaa)DE-He213 MSCs (dpeaa)DE-He213 Hepatic fibrosis (dpeaa)DE-He213 Liver cirrhosis (dpeaa)DE-He213 Carbon tetrachloride (dpeaa)DE-He213 George, Joseph (orcid)0000-0001-5354-7884 aut Hashizume, Chieko aut Saito, Takashi aut Ueda, Yoshimichi aut Ishigaki, Yasuhito aut Tsuchishima, Mutsumi aut Tsutsumi, Mikihiro (orcid)0000-0003-0463-074X aut Enthalten in Molecular medicine [London] : BioMed Central, 1994 28(2022), 1 vom: 29. Nov. (DE-627)269539611 (DE-600)1475577-4 1528-3658 nnns volume:28 year:2022 number:1 day:29 month:11 https://dx.doi.org/10.1186/s10020-022-00566-6 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2153 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2022 1 29 11
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Here, we evaluated the effects of human adipose derived stem cells (hADSCs) to attenuate experimentally induced hepatic fibrosis and early cirrhosis in rats. Methods Hepatic fibrosis was induced by intraperitoneal injections of $ CCl_{4} $ (0.1 ml/100 g body weight) twice a week for 8 weeks. hADSCs were isolated and cultured on polyethylene discs coated with hydroxyapatite and 2 cm diameter disc was surgically implanted on the right lateral lobe of the liver. Discs implanted without hADSCs served as control. The animals were injected again with $ CCl_{4} $ once a week for another 8 weeks. All the animals were sacrificed at the end of 16th week. Results Serial administrations of $ CCl_{4} $ resulted in well developed fibrosis and early cirrhosis at 8th week which maintained until the 16th week. Animals treated with hADSC discs depicted over 50% decrease of collagen with significant increase in serum albumin and total protein levels. Immunohistochemical staining for TGF-β1, α-smooth muscle actin, and collagen type I and type III demonstrated marked decrease compared to the animals without hADSC treatment. Conclusions Treatment with hADSCs improved liver functions, markedly reduced hepatic fibrosis and early cirrhosis. Various pleiotropic and paracrine factors secreted from the hADSCs seem to serve as reparative functions in the attenuation of liver cirrhosis. 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author	Nomura, Masateru
spellingShingle	Nomura, Masateru misc Adipose derived stem cells misc hADSCs misc Mesenchymal stem cells misc MSCs misc Hepatic fibrosis misc Liver cirrhosis misc Carbon tetrachloride Surgical implantation of human adipose derived stem cells attenuates experimentally induced hepatic fibrosis in rats
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topic_title	Surgical implantation of human adipose derived stem cells attenuates experimentally induced hepatic fibrosis in rats Adipose derived stem cells (dpeaa)DE-He213 hADSCs (dpeaa)DE-He213 Mesenchymal stem cells (dpeaa)DE-He213 MSCs (dpeaa)DE-He213 Hepatic fibrosis (dpeaa)DE-He213 Liver cirrhosis (dpeaa)DE-He213 Carbon tetrachloride (dpeaa)DE-He213
topic	misc Adipose derived stem cells misc hADSCs misc Mesenchymal stem cells misc MSCs misc Hepatic fibrosis misc Liver cirrhosis misc Carbon tetrachloride
topic_unstemmed	misc Adipose derived stem cells misc hADSCs misc Mesenchymal stem cells misc MSCs misc Hepatic fibrosis misc Liver cirrhosis misc Carbon tetrachloride
topic_browse	misc Adipose derived stem cells misc hADSCs misc Mesenchymal stem cells misc MSCs misc Hepatic fibrosis misc Liver cirrhosis misc Carbon tetrachloride
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title	Surgical implantation of human adipose derived stem cells attenuates experimentally induced hepatic fibrosis in rats
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title_full	Surgical implantation of human adipose derived stem cells attenuates experimentally induced hepatic fibrosis in rats
author_sort	Nomura, Masateru
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author_browse	Nomura, Masateru George, Joseph Hashizume, Chieko Saito, Takashi Ueda, Yoshimichi Ishigaki, Yasuhito Tsuchishima, Mutsumi Tsutsumi, Mikihiro
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title_sort	surgical implantation of human adipose derived stem cells attenuates experimentally induced hepatic fibrosis in rats
title_auth	Surgical implantation of human adipose derived stem cells attenuates experimentally induced hepatic fibrosis in rats
abstract	Background Mesenchymal stem cells (MSCs) are multipotent stromal cells and could exert hepatoprotective effects against acute liver injury, steatohepatitis, and fibrogenesis. Here, we evaluated the effects of human adipose derived stem cells (hADSCs) to attenuate experimentally induced hepatic fibrosis and early cirrhosis in rats. Methods Hepatic fibrosis was induced by intraperitoneal injections of $ CCl_{4} $ (0.1 ml/100 g body weight) twice a week for 8 weeks. hADSCs were isolated and cultured on polyethylene discs coated with hydroxyapatite and 2 cm diameter disc was surgically implanted on the right lateral lobe of the liver. Discs implanted without hADSCs served as control. The animals were injected again with $ CCl_{4} $ once a week for another 8 weeks. All the animals were sacrificed at the end of 16th week. Results Serial administrations of $ CCl_{4} $ resulted in well developed fibrosis and early cirrhosis at 8th week which maintained until the 16th week. Animals treated with hADSC discs depicted over 50% decrease of collagen with significant increase in serum albumin and total protein levels. Immunohistochemical staining for TGF-β1, α-smooth muscle actin, and collagen type I and type III demonstrated marked decrease compared to the animals without hADSC treatment. Conclusions Treatment with hADSCs improved liver functions, markedly reduced hepatic fibrosis and early cirrhosis. Various pleiotropic and paracrine factors secreted from the hADSCs seem to serve as reparative functions in the attenuation of liver cirrhosis. The data demonstrated that treatment with hADSCs can be successfully used as a potent therapeutic method to prevent progression of hepatic fibrosis and related adverse events. © The Author(s) 2022
abstractGer	Background Mesenchymal stem cells (MSCs) are multipotent stromal cells and could exert hepatoprotective effects against acute liver injury, steatohepatitis, and fibrogenesis. Here, we evaluated the effects of human adipose derived stem cells (hADSCs) to attenuate experimentally induced hepatic fibrosis and early cirrhosis in rats. Methods Hepatic fibrosis was induced by intraperitoneal injections of $ CCl_{4} $ (0.1 ml/100 g body weight) twice a week for 8 weeks. hADSCs were isolated and cultured on polyethylene discs coated with hydroxyapatite and 2 cm diameter disc was surgically implanted on the right lateral lobe of the liver. Discs implanted without hADSCs served as control. The animals were injected again with $ CCl_{4} $ once a week for another 8 weeks. All the animals were sacrificed at the end of 16th week. Results Serial administrations of $ CCl_{4} $ resulted in well developed fibrosis and early cirrhosis at 8th week which maintained until the 16th week. Animals treated with hADSC discs depicted over 50% decrease of collagen with significant increase in serum albumin and total protein levels. Immunohistochemical staining for TGF-β1, α-smooth muscle actin, and collagen type I and type III demonstrated marked decrease compared to the animals without hADSC treatment. Conclusions Treatment with hADSCs improved liver functions, markedly reduced hepatic fibrosis and early cirrhosis. Various pleiotropic and paracrine factors secreted from the hADSCs seem to serve as reparative functions in the attenuation of liver cirrhosis. The data demonstrated that treatment with hADSCs can be successfully used as a potent therapeutic method to prevent progression of hepatic fibrosis and related adverse events. © The Author(s) 2022
abstract_unstemmed	Background Mesenchymal stem cells (MSCs) are multipotent stromal cells and could exert hepatoprotective effects against acute liver injury, steatohepatitis, and fibrogenesis. Here, we evaluated the effects of human adipose derived stem cells (hADSCs) to attenuate experimentally induced hepatic fibrosis and early cirrhosis in rats. Methods Hepatic fibrosis was induced by intraperitoneal injections of $ CCl_{4} $ (0.1 ml/100 g body weight) twice a week for 8 weeks. hADSCs were isolated and cultured on polyethylene discs coated with hydroxyapatite and 2 cm diameter disc was surgically implanted on the right lateral lobe of the liver. Discs implanted without hADSCs served as control. The animals were injected again with $ CCl_{4} $ once a week for another 8 weeks. All the animals were sacrificed at the end of 16th week. Results Serial administrations of $ CCl_{4} $ resulted in well developed fibrosis and early cirrhosis at 8th week which maintained until the 16th week. Animals treated with hADSC discs depicted over 50% decrease of collagen with significant increase in serum albumin and total protein levels. Immunohistochemical staining for TGF-β1, α-smooth muscle actin, and collagen type I and type III demonstrated marked decrease compared to the animals without hADSC treatment. Conclusions Treatment with hADSCs improved liver functions, markedly reduced hepatic fibrosis and early cirrhosis. Various pleiotropic and paracrine factors secreted from the hADSCs seem to serve as reparative functions in the attenuation of liver cirrhosis. The data demonstrated that treatment with hADSCs can be successfully used as a potent therapeutic method to prevent progression of hepatic fibrosis and related adverse events. © The Author(s) 2022
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title_short	Surgical implantation of human adipose derived stem cells attenuates experimentally induced hepatic fibrosis in rats
url	https://dx.doi.org/10.1186/s10020-022-00566-6
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author2	George, Joseph Hashizume, Chieko Saito, Takashi Ueda, Yoshimichi Ishigaki, Yasuhito Tsuchishima, Mutsumi Tsutsumi, Mikihiro
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up_date	2024-07-03T20:17:55.198Z
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Here, we evaluated the effects of human adipose derived stem cells (hADSCs) to attenuate experimentally induced hepatic fibrosis and early cirrhosis in rats. Methods Hepatic fibrosis was induced by intraperitoneal injections of $ CCl_{4} $ (0.1 ml/100 g body weight) twice a week for 8 weeks. hADSCs were isolated and cultured on polyethylene discs coated with hydroxyapatite and 2 cm diameter disc was surgically implanted on the right lateral lobe of the liver. Discs implanted without hADSCs served as control. The animals were injected again with $ CCl_{4} $ once a week for another 8 weeks. All the animals were sacrificed at the end of 16th week. Results Serial administrations of $ CCl_{4} $ resulted in well developed fibrosis and early cirrhosis at 8th week which maintained until the 16th week. Animals treated with hADSC discs depicted over 50% decrease of collagen with significant increase in serum albumin and total protein levels. Immunohistochemical staining for TGF-β1, α-smooth muscle actin, and collagen type I and type III demonstrated marked decrease compared to the animals without hADSC treatment. Conclusions Treatment with hADSCs improved liver functions, markedly reduced hepatic fibrosis and early cirrhosis. Various pleiotropic and paracrine factors secreted from the hADSCs seem to serve as reparative functions in the attenuation of liver cirrhosis. The data demonstrated that treatment with hADSCs can be successfully used as a potent therapeutic method to prevent progression of hepatic fibrosis and related adverse events.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Adipose derived stem cells</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">hADSCs</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Mesenchymal stem cells</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">MSCs</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Hepatic fibrosis</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Liver cirrhosis</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Carbon tetrachloride</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">George, Joseph</subfield><subfield code="0">(orcid)0000-0001-5354-7884</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hashizume, Chieko</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Saito, Takashi</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ueda, Yoshimichi</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ishigaki, Yasuhito</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Tsuchishima, Mutsumi</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Tsutsumi, Mikihiro</subfield><subfield code="0">(orcid)0000-0003-0463-074X</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Molecular medicine</subfield><subfield code="d">[London] : BioMed Central, 1994</subfield><subfield code="g">28(2022), 1 vom: 29. 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Surgical implantation of human adipose derived stem cells attenuates experimentally induced hepatic fibrosis in rats

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