Activation of Heme Oxygenase-1 is Involved in the Preventive Effect of Honokiol against Oxidative Damage in Human Retinal Pigment Epithelial Cells
Abstract Previous studies have shown that honokiol, a bioactive compound originated from the Magnolia, has beneficial effects on various chronic diseases. Although our previous study has demonstrated that honokiol effectively protects C2C12 myoblasts against hydrogen peroxide ($ H_{2} %$ O_{2} $)-in...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Hong, Su Hyun [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022 |
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| Schlagwörter: |
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| Anmerkung: |
© The Korean Society for Biotechnology and Bioengineering and Springer 2022 |
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| Übergeordnetes Werk: |
Enthalten in: Biotechnology and bioprocess engineering - Seoul : Society, 1996, 27(2022), 6 vom: 17. Nov., Seite 975-986 |
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| Übergeordnetes Werk: |
volume:27 ; year:2022 ; number:6 ; day:17 ; month:11 ; pages:975-986 |
| Links: |
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| DOI / URN: |
10.1007/s12257-022-0174-x |
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| Katalog-ID: |
SPR051313073 |
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| 245 | 1 | 0 | |a Activation of Heme Oxygenase-1 is Involved in the Preventive Effect of Honokiol against Oxidative Damage in Human Retinal Pigment Epithelial Cells |
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| 520 | |a Abstract Previous studies have shown that honokiol, a bioactive compound originated from the Magnolia, has beneficial effects on various chronic diseases. Although our previous study has demonstrated that honokiol effectively protects C2C12 myoblasts against hydrogen peroxide ($ H_{2} %$ O_{2} $)-induced oxidative stress and cytotoxicity, the antioxidant mechanism in human retinal pigment epithelial (RPE) cells has not been reported yet. Therefore, this study investigates whether honokiol attenuate cytotoxicity of $ H_{2} %$ O_{2} $ to human RPE ARPE-19 cells. Our results showed that $ H_{2} %$ O_{2} $-induced loss of cell viability and increase of reactive oxygen species production in ARPE-19 cells were significantly alleviated by honokiol, which was associated with increased phosphorylation of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulation of heme oxygenase-1 (HO-1). In addition, honokiol markedly attenuated DNA damage and G2/M phase cell cycle arrest and improved apoptosis in $ H_{2} %$ O_{2} $-treated ARPE-19 cells. Furthermore, mitochondrial dysfunction by $ H_{2} %$ O_{2} $ was reversed by honokiol through preservation of mitochondrial membrane potential, decrease of Bax/Bcl-2 expression ratio, and inhibition of cytochrome c release into the cytoplasm. However, zinc protoporphyrin, an inhibitor of HO-1, significantly abrogated these preventive effects, suggesting a critical role of Nrf2/HO-1 in the antioxidant activity of honokiol. Taken together, these results demonstrate that activation of Nrf2/HO-1 antioxidant signaling by honokiol could rescue ARPE-19 cells from oxidative damage. | ||
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| 650 | 4 | |a reactive oxygen species |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a retinal pigment epithelial cells |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Park, Cheol |4 aut | |
| 700 | 1 | |a Hwangbo, Hyun |4 aut | |
| 700 | 1 | |a Bang, EunJin |4 aut | |
| 700 | 1 | |a Kim, Sung Ok |4 aut | |
| 700 | 1 | |a Shim, Jung-Hyun |4 aut | |
| 700 | 1 | |a Park, Shin-Hyung |4 aut | |
| 700 | 1 | |a Lee, Hyesook |4 aut | |
| 700 | 1 | |a Leem, Sun-Hee |4 aut | |
| 700 | 1 | |a Kim, Gi-Young |4 aut | |
| 700 | 1 | |a Choi, Yung Hyun |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biotechnology and bioprocess engineering |d Seoul : Society, 1996 |g 27(2022), 6 vom: 17. Nov., Seite 975-986 |w (DE-627)373321821 |w (DE-600)2125481-3 |x 1976-3816 |7 nnns |
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10.1007/s12257-022-0174-x doi (DE-627)SPR051313073 (SPR)s12257-022-0174-x-e DE-627 ger DE-627 rakwb eng Hong, Su Hyun verfasserin aut Activation of Heme Oxygenase-1 is Involved in the Preventive Effect of Honokiol against Oxidative Damage in Human Retinal Pigment Epithelial Cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Society for Biotechnology and Bioengineering and Springer 2022 Abstract Previous studies have shown that honokiol, a bioactive compound originated from the Magnolia, has beneficial effects on various chronic diseases. Although our previous study has demonstrated that honokiol effectively protects C2C12 myoblasts against hydrogen peroxide ($ H_{2} %$ O_{2} $)-induced oxidative stress and cytotoxicity, the antioxidant mechanism in human retinal pigment epithelial (RPE) cells has not been reported yet. Therefore, this study investigates whether honokiol attenuate cytotoxicity of $ H_{2} %$ O_{2} $ to human RPE ARPE-19 cells. Our results showed that $ H_{2} %$ O_{2} $-induced loss of cell viability and increase of reactive oxygen species production in ARPE-19 cells were significantly alleviated by honokiol, which was associated with increased phosphorylation of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulation of heme oxygenase-1 (HO-1). In addition, honokiol markedly attenuated DNA damage and G2/M phase cell cycle arrest and improved apoptosis in $ H_{2} %$ O_{2} $-treated ARPE-19 cells. Furthermore, mitochondrial dysfunction by $ H_{2} %$ O_{2} $ was reversed by honokiol through preservation of mitochondrial membrane potential, decrease of Bax/Bcl-2 expression ratio, and inhibition of cytochrome c release into the cytoplasm. However, zinc protoporphyrin, an inhibitor of HO-1, significantly abrogated these preventive effects, suggesting a critical role of Nrf2/HO-1 in the antioxidant activity of honokiol. Taken together, these results demonstrate that activation of Nrf2/HO-1 antioxidant signaling by honokiol could rescue ARPE-19 cells from oxidative damage. honokiol (dpeaa)DE-He213 Nrf2/HO-1 (dpeaa)DE-He213 reactive oxygen species (dpeaa)DE-He213 retinal pigment epithelial cells (dpeaa)DE-He213 Park, Cheol aut Hwangbo, Hyun aut Bang, EunJin aut Kim, Sung Ok aut Shim, Jung-Hyun aut Park, Shin-Hyung aut Lee, Hyesook aut Leem, Sun-Hee aut Kim, Gi-Young aut Choi, Yung Hyun aut Enthalten in Biotechnology and bioprocess engineering Seoul : Society, 1996 27(2022), 6 vom: 17. Nov., Seite 975-986 (DE-627)373321821 (DE-600)2125481-3 1976-3816 nnns volume:27 year:2022 number:6 day:17 month:11 pages:975-986 https://dx.doi.org/10.1007/s12257-022-0174-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 27 2022 6 17 11 975-986 |
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10.1007/s12257-022-0174-x doi (DE-627)SPR051313073 (SPR)s12257-022-0174-x-e DE-627 ger DE-627 rakwb eng Hong, Su Hyun verfasserin aut Activation of Heme Oxygenase-1 is Involved in the Preventive Effect of Honokiol against Oxidative Damage in Human Retinal Pigment Epithelial Cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Society for Biotechnology and Bioengineering and Springer 2022 Abstract Previous studies have shown that honokiol, a bioactive compound originated from the Magnolia, has beneficial effects on various chronic diseases. Although our previous study has demonstrated that honokiol effectively protects C2C12 myoblasts against hydrogen peroxide ($ H_{2} %$ O_{2} $)-induced oxidative stress and cytotoxicity, the antioxidant mechanism in human retinal pigment epithelial (RPE) cells has not been reported yet. Therefore, this study investigates whether honokiol attenuate cytotoxicity of $ H_{2} %$ O_{2} $ to human RPE ARPE-19 cells. Our results showed that $ H_{2} %$ O_{2} $-induced loss of cell viability and increase of reactive oxygen species production in ARPE-19 cells were significantly alleviated by honokiol, which was associated with increased phosphorylation of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulation of heme oxygenase-1 (HO-1). In addition, honokiol markedly attenuated DNA damage and G2/M phase cell cycle arrest and improved apoptosis in $ H_{2} %$ O_{2} $-treated ARPE-19 cells. Furthermore, mitochondrial dysfunction by $ H_{2} %$ O_{2} $ was reversed by honokiol through preservation of mitochondrial membrane potential, decrease of Bax/Bcl-2 expression ratio, and inhibition of cytochrome c release into the cytoplasm. However, zinc protoporphyrin, an inhibitor of HO-1, significantly abrogated these preventive effects, suggesting a critical role of Nrf2/HO-1 in the antioxidant activity of honokiol. Taken together, these results demonstrate that activation of Nrf2/HO-1 antioxidant signaling by honokiol could rescue ARPE-19 cells from oxidative damage. honokiol (dpeaa)DE-He213 Nrf2/HO-1 (dpeaa)DE-He213 reactive oxygen species (dpeaa)DE-He213 retinal pigment epithelial cells (dpeaa)DE-He213 Park, Cheol aut Hwangbo, Hyun aut Bang, EunJin aut Kim, Sung Ok aut Shim, Jung-Hyun aut Park, Shin-Hyung aut Lee, Hyesook aut Leem, Sun-Hee aut Kim, Gi-Young aut Choi, Yung Hyun aut Enthalten in Biotechnology and bioprocess engineering Seoul : Society, 1996 27(2022), 6 vom: 17. Nov., Seite 975-986 (DE-627)373321821 (DE-600)2125481-3 1976-3816 nnns volume:27 year:2022 number:6 day:17 month:11 pages:975-986 https://dx.doi.org/10.1007/s12257-022-0174-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 27 2022 6 17 11 975-986 |
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10.1007/s12257-022-0174-x doi (DE-627)SPR051313073 (SPR)s12257-022-0174-x-e DE-627 ger DE-627 rakwb eng Hong, Su Hyun verfasserin aut Activation of Heme Oxygenase-1 is Involved in the Preventive Effect of Honokiol against Oxidative Damage in Human Retinal Pigment Epithelial Cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Society for Biotechnology and Bioengineering and Springer 2022 Abstract Previous studies have shown that honokiol, a bioactive compound originated from the Magnolia, has beneficial effects on various chronic diseases. Although our previous study has demonstrated that honokiol effectively protects C2C12 myoblasts against hydrogen peroxide ($ H_{2} %$ O_{2} $)-induced oxidative stress and cytotoxicity, the antioxidant mechanism in human retinal pigment epithelial (RPE) cells has not been reported yet. Therefore, this study investigates whether honokiol attenuate cytotoxicity of $ H_{2} %$ O_{2} $ to human RPE ARPE-19 cells. Our results showed that $ H_{2} %$ O_{2} $-induced loss of cell viability and increase of reactive oxygen species production in ARPE-19 cells were significantly alleviated by honokiol, which was associated with increased phosphorylation of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulation of heme oxygenase-1 (HO-1). In addition, honokiol markedly attenuated DNA damage and G2/M phase cell cycle arrest and improved apoptosis in $ H_{2} %$ O_{2} $-treated ARPE-19 cells. Furthermore, mitochondrial dysfunction by $ H_{2} %$ O_{2} $ was reversed by honokiol through preservation of mitochondrial membrane potential, decrease of Bax/Bcl-2 expression ratio, and inhibition of cytochrome c release into the cytoplasm. However, zinc protoporphyrin, an inhibitor of HO-1, significantly abrogated these preventive effects, suggesting a critical role of Nrf2/HO-1 in the antioxidant activity of honokiol. Taken together, these results demonstrate that activation of Nrf2/HO-1 antioxidant signaling by honokiol could rescue ARPE-19 cells from oxidative damage. honokiol (dpeaa)DE-He213 Nrf2/HO-1 (dpeaa)DE-He213 reactive oxygen species (dpeaa)DE-He213 retinal pigment epithelial cells (dpeaa)DE-He213 Park, Cheol aut Hwangbo, Hyun aut Bang, EunJin aut Kim, Sung Ok aut Shim, Jung-Hyun aut Park, Shin-Hyung aut Lee, Hyesook aut Leem, Sun-Hee aut Kim, Gi-Young aut Choi, Yung Hyun aut Enthalten in Biotechnology and bioprocess engineering Seoul : Society, 1996 27(2022), 6 vom: 17. Nov., Seite 975-986 (DE-627)373321821 (DE-600)2125481-3 1976-3816 nnns volume:27 year:2022 number:6 day:17 month:11 pages:975-986 https://dx.doi.org/10.1007/s12257-022-0174-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 27 2022 6 17 11 975-986 |
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10.1007/s12257-022-0174-x doi (DE-627)SPR051313073 (SPR)s12257-022-0174-x-e DE-627 ger DE-627 rakwb eng Hong, Su Hyun verfasserin aut Activation of Heme Oxygenase-1 is Involved in the Preventive Effect of Honokiol against Oxidative Damage in Human Retinal Pigment Epithelial Cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Society for Biotechnology and Bioengineering and Springer 2022 Abstract Previous studies have shown that honokiol, a bioactive compound originated from the Magnolia, has beneficial effects on various chronic diseases. Although our previous study has demonstrated that honokiol effectively protects C2C12 myoblasts against hydrogen peroxide ($ H_{2} %$ O_{2} $)-induced oxidative stress and cytotoxicity, the antioxidant mechanism in human retinal pigment epithelial (RPE) cells has not been reported yet. Therefore, this study investigates whether honokiol attenuate cytotoxicity of $ H_{2} %$ O_{2} $ to human RPE ARPE-19 cells. Our results showed that $ H_{2} %$ O_{2} $-induced loss of cell viability and increase of reactive oxygen species production in ARPE-19 cells were significantly alleviated by honokiol, which was associated with increased phosphorylation of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulation of heme oxygenase-1 (HO-1). In addition, honokiol markedly attenuated DNA damage and G2/M phase cell cycle arrest and improved apoptosis in $ H_{2} %$ O_{2} $-treated ARPE-19 cells. Furthermore, mitochondrial dysfunction by $ H_{2} %$ O_{2} $ was reversed by honokiol through preservation of mitochondrial membrane potential, decrease of Bax/Bcl-2 expression ratio, and inhibition of cytochrome c release into the cytoplasm. However, zinc protoporphyrin, an inhibitor of HO-1, significantly abrogated these preventive effects, suggesting a critical role of Nrf2/HO-1 in the antioxidant activity of honokiol. Taken together, these results demonstrate that activation of Nrf2/HO-1 antioxidant signaling by honokiol could rescue ARPE-19 cells from oxidative damage. honokiol (dpeaa)DE-He213 Nrf2/HO-1 (dpeaa)DE-He213 reactive oxygen species (dpeaa)DE-He213 retinal pigment epithelial cells (dpeaa)DE-He213 Park, Cheol aut Hwangbo, Hyun aut Bang, EunJin aut Kim, Sung Ok aut Shim, Jung-Hyun aut Park, Shin-Hyung aut Lee, Hyesook aut Leem, Sun-Hee aut Kim, Gi-Young aut Choi, Yung Hyun aut Enthalten in Biotechnology and bioprocess engineering Seoul : Society, 1996 27(2022), 6 vom: 17. Nov., Seite 975-986 (DE-627)373321821 (DE-600)2125481-3 1976-3816 nnns volume:27 year:2022 number:6 day:17 month:11 pages:975-986 https://dx.doi.org/10.1007/s12257-022-0174-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 27 2022 6 17 11 975-986 |
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10.1007/s12257-022-0174-x doi (DE-627)SPR051313073 (SPR)s12257-022-0174-x-e DE-627 ger DE-627 rakwb eng Hong, Su Hyun verfasserin aut Activation of Heme Oxygenase-1 is Involved in the Preventive Effect of Honokiol against Oxidative Damage in Human Retinal Pigment Epithelial Cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Society for Biotechnology and Bioengineering and Springer 2022 Abstract Previous studies have shown that honokiol, a bioactive compound originated from the Magnolia, has beneficial effects on various chronic diseases. Although our previous study has demonstrated that honokiol effectively protects C2C12 myoblasts against hydrogen peroxide ($ H_{2} %$ O_{2} $)-induced oxidative stress and cytotoxicity, the antioxidant mechanism in human retinal pigment epithelial (RPE) cells has not been reported yet. Therefore, this study investigates whether honokiol attenuate cytotoxicity of $ H_{2} %$ O_{2} $ to human RPE ARPE-19 cells. Our results showed that $ H_{2} %$ O_{2} $-induced loss of cell viability and increase of reactive oxygen species production in ARPE-19 cells were significantly alleviated by honokiol, which was associated with increased phosphorylation of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulation of heme oxygenase-1 (HO-1). In addition, honokiol markedly attenuated DNA damage and G2/M phase cell cycle arrest and improved apoptosis in $ H_{2} %$ O_{2} $-treated ARPE-19 cells. Furthermore, mitochondrial dysfunction by $ H_{2} %$ O_{2} $ was reversed by honokiol through preservation of mitochondrial membrane potential, decrease of Bax/Bcl-2 expression ratio, and inhibition of cytochrome c release into the cytoplasm. However, zinc protoporphyrin, an inhibitor of HO-1, significantly abrogated these preventive effects, suggesting a critical role of Nrf2/HO-1 in the antioxidant activity of honokiol. Taken together, these results demonstrate that activation of Nrf2/HO-1 antioxidant signaling by honokiol could rescue ARPE-19 cells from oxidative damage. honokiol (dpeaa)DE-He213 Nrf2/HO-1 (dpeaa)DE-He213 reactive oxygen species (dpeaa)DE-He213 retinal pigment epithelial cells (dpeaa)DE-He213 Park, Cheol aut Hwangbo, Hyun aut Bang, EunJin aut Kim, Sung Ok aut Shim, Jung-Hyun aut Park, Shin-Hyung aut Lee, Hyesook aut Leem, Sun-Hee aut Kim, Gi-Young aut Choi, Yung Hyun aut Enthalten in Biotechnology and bioprocess engineering Seoul : Society, 1996 27(2022), 6 vom: 17. Nov., Seite 975-986 (DE-627)373321821 (DE-600)2125481-3 1976-3816 nnns volume:27 year:2022 number:6 day:17 month:11 pages:975-986 https://dx.doi.org/10.1007/s12257-022-0174-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 27 2022 6 17 11 975-986 |
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Enthalten in Biotechnology and bioprocess engineering 27(2022), 6 vom: 17. Nov., Seite 975-986 volume:27 year:2022 number:6 day:17 month:11 pages:975-986 |
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Enthalten in Biotechnology and bioprocess engineering 27(2022), 6 vom: 17. Nov., Seite 975-986 volume:27 year:2022 number:6 day:17 month:11 pages:975-986 |
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honokiol Nrf2/HO-1 reactive oxygen species retinal pigment epithelial cells |
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Hong, Su Hyun @@aut@@ Park, Cheol @@aut@@ Hwangbo, Hyun @@aut@@ Bang, EunJin @@aut@@ Kim, Sung Ok @@aut@@ Shim, Jung-Hyun @@aut@@ Park, Shin-Hyung @@aut@@ Lee, Hyesook @@aut@@ Leem, Sun-Hee @@aut@@ Kim, Gi-Young @@aut@@ Choi, Yung Hyun @@aut@@ |
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2022-11-17T00:00:00Z |
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Although our previous study has demonstrated that honokiol effectively protects C2C12 myoblasts against hydrogen peroxide ($ H_{2} %$ O_{2} $)-induced oxidative stress and cytotoxicity, the antioxidant mechanism in human retinal pigment epithelial (RPE) cells has not been reported yet. Therefore, this study investigates whether honokiol attenuate cytotoxicity of $ H_{2} %$ O_{2} $ to human RPE ARPE-19 cells. Our results showed that $ H_{2} %$ O_{2} $-induced loss of cell viability and increase of reactive oxygen species production in ARPE-19 cells were significantly alleviated by honokiol, which was associated with increased phosphorylation of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulation of heme oxygenase-1 (HO-1). In addition, honokiol markedly attenuated DNA damage and G2/M phase cell cycle arrest and improved apoptosis in $ H_{2} %$ O_{2} $-treated ARPE-19 cells. Furthermore, mitochondrial dysfunction by $ H_{2} %$ O_{2} $ was reversed by honokiol through preservation of mitochondrial membrane potential, decrease of Bax/Bcl-2 expression ratio, and inhibition of cytochrome c release into the cytoplasm. However, zinc protoporphyrin, an inhibitor of HO-1, significantly abrogated these preventive effects, suggesting a critical role of Nrf2/HO-1 in the antioxidant activity of honokiol. 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Hong, Su Hyun |
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Hong, Su Hyun misc honokiol misc Nrf2/HO-1 misc reactive oxygen species misc retinal pigment epithelial cells Activation of Heme Oxygenase-1 is Involved in the Preventive Effect of Honokiol against Oxidative Damage in Human Retinal Pigment Epithelial Cells |
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Activation of Heme Oxygenase-1 is Involved in the Preventive Effect of Honokiol against Oxidative Damage in Human Retinal Pigment Epithelial Cells honokiol (dpeaa)DE-He213 Nrf2/HO-1 (dpeaa)DE-He213 reactive oxygen species (dpeaa)DE-He213 retinal pigment epithelial cells (dpeaa)DE-He213 |
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Activation of Heme Oxygenase-1 is Involved in the Preventive Effect of Honokiol against Oxidative Damage in Human Retinal Pigment Epithelial Cells |
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Hong, Su Hyun Park, Cheol Hwangbo, Hyun Bang, EunJin Kim, Sung Ok Shim, Jung-Hyun Park, Shin-Hyung Lee, Hyesook Leem, Sun-Hee Kim, Gi-Young Choi, Yung Hyun |
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activation of heme oxygenase-1 is involved in the preventive effect of honokiol against oxidative damage in human retinal pigment epithelial cells |
| title_auth |
Activation of Heme Oxygenase-1 is Involved in the Preventive Effect of Honokiol against Oxidative Damage in Human Retinal Pigment Epithelial Cells |
| abstract |
Abstract Previous studies have shown that honokiol, a bioactive compound originated from the Magnolia, has beneficial effects on various chronic diseases. Although our previous study has demonstrated that honokiol effectively protects C2C12 myoblasts against hydrogen peroxide ($ H_{2} %$ O_{2} $)-induced oxidative stress and cytotoxicity, the antioxidant mechanism in human retinal pigment epithelial (RPE) cells has not been reported yet. Therefore, this study investigates whether honokiol attenuate cytotoxicity of $ H_{2} %$ O_{2} $ to human RPE ARPE-19 cells. Our results showed that $ H_{2} %$ O_{2} $-induced loss of cell viability and increase of reactive oxygen species production in ARPE-19 cells were significantly alleviated by honokiol, which was associated with increased phosphorylation of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulation of heme oxygenase-1 (HO-1). In addition, honokiol markedly attenuated DNA damage and G2/M phase cell cycle arrest and improved apoptosis in $ H_{2} %$ O_{2} $-treated ARPE-19 cells. Furthermore, mitochondrial dysfunction by $ H_{2} %$ O_{2} $ was reversed by honokiol through preservation of mitochondrial membrane potential, decrease of Bax/Bcl-2 expression ratio, and inhibition of cytochrome c release into the cytoplasm. However, zinc protoporphyrin, an inhibitor of HO-1, significantly abrogated these preventive effects, suggesting a critical role of Nrf2/HO-1 in the antioxidant activity of honokiol. Taken together, these results demonstrate that activation of Nrf2/HO-1 antioxidant signaling by honokiol could rescue ARPE-19 cells from oxidative damage. © The Korean Society for Biotechnology and Bioengineering and Springer 2022 |
| abstractGer |
Abstract Previous studies have shown that honokiol, a bioactive compound originated from the Magnolia, has beneficial effects on various chronic diseases. Although our previous study has demonstrated that honokiol effectively protects C2C12 myoblasts against hydrogen peroxide ($ H_{2} %$ O_{2} $)-induced oxidative stress and cytotoxicity, the antioxidant mechanism in human retinal pigment epithelial (RPE) cells has not been reported yet. Therefore, this study investigates whether honokiol attenuate cytotoxicity of $ H_{2} %$ O_{2} $ to human RPE ARPE-19 cells. Our results showed that $ H_{2} %$ O_{2} $-induced loss of cell viability and increase of reactive oxygen species production in ARPE-19 cells were significantly alleviated by honokiol, which was associated with increased phosphorylation of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulation of heme oxygenase-1 (HO-1). In addition, honokiol markedly attenuated DNA damage and G2/M phase cell cycle arrest and improved apoptosis in $ H_{2} %$ O_{2} $-treated ARPE-19 cells. Furthermore, mitochondrial dysfunction by $ H_{2} %$ O_{2} $ was reversed by honokiol through preservation of mitochondrial membrane potential, decrease of Bax/Bcl-2 expression ratio, and inhibition of cytochrome c release into the cytoplasm. However, zinc protoporphyrin, an inhibitor of HO-1, significantly abrogated these preventive effects, suggesting a critical role of Nrf2/HO-1 in the antioxidant activity of honokiol. Taken together, these results demonstrate that activation of Nrf2/HO-1 antioxidant signaling by honokiol could rescue ARPE-19 cells from oxidative damage. © The Korean Society for Biotechnology and Bioengineering and Springer 2022 |
| abstract_unstemmed |
Abstract Previous studies have shown that honokiol, a bioactive compound originated from the Magnolia, has beneficial effects on various chronic diseases. Although our previous study has demonstrated that honokiol effectively protects C2C12 myoblasts against hydrogen peroxide ($ H_{2} %$ O_{2} $)-induced oxidative stress and cytotoxicity, the antioxidant mechanism in human retinal pigment epithelial (RPE) cells has not been reported yet. Therefore, this study investigates whether honokiol attenuate cytotoxicity of $ H_{2} %$ O_{2} $ to human RPE ARPE-19 cells. Our results showed that $ H_{2} %$ O_{2} $-induced loss of cell viability and increase of reactive oxygen species production in ARPE-19 cells were significantly alleviated by honokiol, which was associated with increased phosphorylation of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulation of heme oxygenase-1 (HO-1). In addition, honokiol markedly attenuated DNA damage and G2/M phase cell cycle arrest and improved apoptosis in $ H_{2} %$ O_{2} $-treated ARPE-19 cells. Furthermore, mitochondrial dysfunction by $ H_{2} %$ O_{2} $ was reversed by honokiol through preservation of mitochondrial membrane potential, decrease of Bax/Bcl-2 expression ratio, and inhibition of cytochrome c release into the cytoplasm. However, zinc protoporphyrin, an inhibitor of HO-1, significantly abrogated these preventive effects, suggesting a critical role of Nrf2/HO-1 in the antioxidant activity of honokiol. Taken together, these results demonstrate that activation of Nrf2/HO-1 antioxidant signaling by honokiol could rescue ARPE-19 cells from oxidative damage. © The Korean Society for Biotechnology and Bioengineering and Springer 2022 |
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| title_short |
Activation of Heme Oxygenase-1 is Involved in the Preventive Effect of Honokiol against Oxidative Damage in Human Retinal Pigment Epithelial Cells |
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https://dx.doi.org/10.1007/s12257-022-0174-x |
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| author2 |
Park, Cheol Hwangbo, Hyun Bang, EunJin Kim, Sung Ok Shim, Jung-Hyun Park, Shin-Hyung Lee, Hyesook Leem, Sun-Hee Kim, Gi-Young Choi, Yung Hyun |
| author2Str |
Park, Cheol Hwangbo, Hyun Bang, EunJin Kim, Sung Ok Shim, Jung-Hyun Park, Shin-Hyung Lee, Hyesook Leem, Sun-Hee Kim, Gi-Young Choi, Yung Hyun |
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2024-07-03T21:04:04.974Z |
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| score |
7.3998213 |