The persistent viral infections in the development and severity of myalgic encephalomyelitis/chronic fatigue syndrome

Background Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a multifactorial disease with an unexplained aetiology in which viral infections are possible trigger factors. The aim of this study was to determine the involvement of human herpesvirus (HHV)-6A/B, HHV-7, and parvovirus B19 (...
Ausführliche Beschreibung

Background Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a multifactorial disease with an unexplained aetiology in which viral infections are possible trigger factors. The aim of this study was to determine the involvement of human herpesvirus (HHV)-6A/B, HHV-7, and parvovirus B19 (B19V) in the etiopathogenesis of ME/CFS. Methods 200 patients with clinically diagnosed ME/CFS and 150 apparently healthy individuals were enrolled in this study. Single-round, nested, and quantitative real-time polymerase chain reactions (PCR) were used to detect the presence and load of HHV-6A/B, HHV-7, and B19V. HHV-6A and HHV-6B were distinguished by PCR and restriction analysis. Immunoenzymatic assays were applied to estimate the presence of virus-specific antibodies and the level of cytokines. Results HHV-6A/B, HHV-7, and B19V specific antibodies were detected among patients and healthy individuals in 92.1% and 76.7%, 84.6% and 93.8%, and 78% and 67.4% of cases. HHV-6B had 99% of HHV-6 positive patients. Latent HHV-6A/B, HHV-7, and B19V infection/co-infection was observed in 51.5% of the patients and 76.7% of the healthy individuals, whereas active–45% of the ME/CFS patients and 8.7% of healthy individuals. HHV-6A/B load in patients with a persistent infection/co-infection in a latent and active phase was 262 and 653.2 copies/$ 10^{6} $ cells, whereas HHV-7 load was 166.5 and 248.5 copies/$ 10^{6} $ cells, and B19V-96.8 and 250.8 copies/$ 10^{6} $ cells, respectively. ME/CFS patients with persistent infection in an active phase had a higher level of pro-inflammatory cytokines (interleukin(IL)-6, tumor necrosis factor-alpha(TNF-α) and IL-12) and anti-inflammatory (IL-10) than with a persistent infection in a latent phase. A significant difference was revealed in the levels of TNF-α, IL-12, and IL-10 among the patient groups without infection, with latent infection/co-infection, active single, double and triple co-infection. The levels of TNF-α, IL-12, and IL-10 are significantly higher in patients with severe compared with a moderate course of ME/CFS. Conclusions Significantly more persistent HHV-6A/B, HHV-7, and B19V infection/co-infection in an active phase with a higher viral load and elevated levels of pro- and anti-inflammatory cytokines among patients with ME/CFS than healthy individuals indicate the importance of these infections/co-infections in ME/CFS development. The presence of these infections/co-infections influences the ME/CFS clinical course severity. Ausführliche Beschreibung

Autor*in:	Rasa-Dzelzkaleja, Santa [verfasserIn] Krumina, Angelika Capenko, Svetlana Nora-Krukle, Zaiga Gravelsina, Sabine Vilmane, Anda Ievina, Lauma Shoenfeld, Yehuda Murovska, Modra

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	Myalgic encephalomyelitis/chronic fatigue syndrome, HHV-6A HHV-6B HHV-7 Human parvovirus B19

Anmerkung:	© The Author(s) 2023

Übergeordnetes Werk:	Enthalten in: Journal of translational medicine - London : BioMed Central, 2003, 21(2023), 1 vom: 18. Jan.
Übergeordnetes Werk:	volume:21 ; year:2023 ; number:1 ; day:18 ; month:01

Links:	Volltext

DOI / URN:	10.1186/s12967-023-03887-0

Katalog-ID:	SPR051360136