iPBS-retrotransposons variations: DNA fingerprinting and the evaluation of genetic diversity and population structure in international cowpea germplasm
Abstract Cowpea belongs to the fabaceae family, originated in Africa, and grown in subtropical and tropical areas worldwide. Cowpea seeds contain high nutritional contents and play a vital role in nutritional feed for animals and humans in many countries. The present investigation aimed to check the...
Ausführliche Beschreibung
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| Autor*in: |
Baloch, Faheem Shehzad [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2023 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| Übergeordnetes Werk: |
Enthalten in: Genetic resources and crop evolution - Dordrecht [u.a.] : Springer Science + Business Media B.V, 1953, 70(2023), 6 vom: 03. Feb., Seite 1867-1877 |
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| Übergeordnetes Werk: |
volume:70 ; year:2023 ; number:6 ; day:03 ; month:02 ; pages:1867-1877 |
| Links: |
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| DOI / URN: |
10.1007/s10722-023-01542-7 |
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SPR051894416 |
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| 520 | |a Abstract Cowpea belongs to the fabaceae family, originated in Africa, and grown in subtropical and tropical areas worldwide. Cowpea seeds contain high nutritional contents and play a vital role in nutritional feed for animals and humans in many countries. The present investigation aimed to check the genetic variations among cowpea germplasm collected from 6 countries using iPBS-retrotransposons markers system. Twelve highly polymorphic iPBS-retrotransposons primers were used for PCR amplification. These primers generated 200 total bands of which 188 were highly polymorphic and 94.30% polymorphism was observed. Mean values of genetic diversity indices i.e., Shannon’s information index (I = 0.452), effective number of alleles (ne = 1.501), overall gene diversity (ht = 0.236), gene diversity (h = 0.298), polymorphism information content (PIC = 0.308), and average genetic distance 0.61 confirmed a great level of genetic variations in studied germplasm. Analysis of molecular variance revealed 96% variation within the population. The STRUCTURE analysis divided germplasm into 2 populations according to geographically. The Neighbor-joining tree and Principle coordinate analysis (PCoA) divided the germplasm into 3 groups and the grouping was mostly aline with STRUCTURE-based clustering. This study revealed high genetic variability in cowpea germplasm and the high values of diversity indices revealed the utility and efficiency of iPBS-retrotransposons marker system. The PCoA, Neighbor-joining tree and STRUCTURE analysis separated the accessions geographically. Analysis of molecular variance revealed 94% genetic variation within a population and Ghana 1 and Turkey 4 had the greatest genetic distance and could be recommended for breeding activities. | ||
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10.1007/s10722-023-01542-7 doi (DE-627)SPR051894416 (SPR)s10722-023-01542-7-e DE-627 ger DE-627 rakwb eng Baloch, Faheem Shehzad verfasserin (orcid)0000-0002-7470-0080 aut iPBS-retrotransposons variations: DNA fingerprinting and the evaluation of genetic diversity and population structure in international cowpea germplasm 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Cowpea belongs to the fabaceae family, originated in Africa, and grown in subtropical and tropical areas worldwide. Cowpea seeds contain high nutritional contents and play a vital role in nutritional feed for animals and humans in many countries. The present investigation aimed to check the genetic variations among cowpea germplasm collected from 6 countries using iPBS-retrotransposons markers system. Twelve highly polymorphic iPBS-retrotransposons primers were used for PCR amplification. These primers generated 200 total bands of which 188 were highly polymorphic and 94.30% polymorphism was observed. Mean values of genetic diversity indices i.e., Shannon’s information index (I = 0.452), effective number of alleles (ne = 1.501), overall gene diversity (ht = 0.236), gene diversity (h = 0.298), polymorphism information content (PIC = 0.308), and average genetic distance 0.61 confirmed a great level of genetic variations in studied germplasm. Analysis of molecular variance revealed 96% variation within the population. The STRUCTURE analysis divided germplasm into 2 populations according to geographically. The Neighbor-joining tree and Principle coordinate analysis (PCoA) divided the germplasm into 3 groups and the grouping was mostly aline with STRUCTURE-based clustering. This study revealed high genetic variability in cowpea germplasm and the high values of diversity indices revealed the utility and efficiency of iPBS-retrotransposons marker system. The PCoA, Neighbor-joining tree and STRUCTURE analysis separated the accessions geographically. Analysis of molecular variance revealed 94% genetic variation within a population and Ghana 1 and Turkey 4 had the greatest genetic distance and could be recommended for breeding activities. iPBS-retrotransposons (dpeaa)DE-He213 Cowpea germplasm (dpeaa)DE-He213 Fabaceae (dpeaa)DE-He213 Population structure (dpeaa)DE-He213 Genetic diversity (dpeaa)DE-He213 Altaf, Muhammad Tanveer aut Bedir, Mehmet aut Nadeem, Muhammad Azhar aut Tatar, Muhammed aut Karaköy, Tolga aut Aasim, Muhammad aut Enthalten in Genetic resources and crop evolution Dordrecht [u.a.] : Springer Science + Business Media B.V, 1953 70(2023), 6 vom: 03. Feb., Seite 1867-1877 (DE-627)320529029 (DE-600)2015535-9 1573-5109 nnns volume:70 year:2023 number:6 day:03 month:02 pages:1867-1877 https://dx.doi.org/10.1007/s10722-023-01542-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 70 2023 6 03 02 1867-1877 |
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10.1007/s10722-023-01542-7 doi (DE-627)SPR051894416 (SPR)s10722-023-01542-7-e DE-627 ger DE-627 rakwb eng Baloch, Faheem Shehzad verfasserin (orcid)0000-0002-7470-0080 aut iPBS-retrotransposons variations: DNA fingerprinting and the evaluation of genetic diversity and population structure in international cowpea germplasm 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Cowpea belongs to the fabaceae family, originated in Africa, and grown in subtropical and tropical areas worldwide. Cowpea seeds contain high nutritional contents and play a vital role in nutritional feed for animals and humans in many countries. The present investigation aimed to check the genetic variations among cowpea germplasm collected from 6 countries using iPBS-retrotransposons markers system. Twelve highly polymorphic iPBS-retrotransposons primers were used for PCR amplification. These primers generated 200 total bands of which 188 were highly polymorphic and 94.30% polymorphism was observed. Mean values of genetic diversity indices i.e., Shannon’s information index (I = 0.452), effective number of alleles (ne = 1.501), overall gene diversity (ht = 0.236), gene diversity (h = 0.298), polymorphism information content (PIC = 0.308), and average genetic distance 0.61 confirmed a great level of genetic variations in studied germplasm. Analysis of molecular variance revealed 96% variation within the population. The STRUCTURE analysis divided germplasm into 2 populations according to geographically. The Neighbor-joining tree and Principle coordinate analysis (PCoA) divided the germplasm into 3 groups and the grouping was mostly aline with STRUCTURE-based clustering. This study revealed high genetic variability in cowpea germplasm and the high values of diversity indices revealed the utility and efficiency of iPBS-retrotransposons marker system. The PCoA, Neighbor-joining tree and STRUCTURE analysis separated the accessions geographically. Analysis of molecular variance revealed 94% genetic variation within a population and Ghana 1 and Turkey 4 had the greatest genetic distance and could be recommended for breeding activities. iPBS-retrotransposons (dpeaa)DE-He213 Cowpea germplasm (dpeaa)DE-He213 Fabaceae (dpeaa)DE-He213 Population structure (dpeaa)DE-He213 Genetic diversity (dpeaa)DE-He213 Altaf, Muhammad Tanveer aut Bedir, Mehmet aut Nadeem, Muhammad Azhar aut Tatar, Muhammed aut Karaköy, Tolga aut Aasim, Muhammad aut Enthalten in Genetic resources and crop evolution Dordrecht [u.a.] : Springer Science + Business Media B.V, 1953 70(2023), 6 vom: 03. Feb., Seite 1867-1877 (DE-627)320529029 (DE-600)2015535-9 1573-5109 nnns volume:70 year:2023 number:6 day:03 month:02 pages:1867-1877 https://dx.doi.org/10.1007/s10722-023-01542-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 70 2023 6 03 02 1867-1877 |
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10.1007/s10722-023-01542-7 doi (DE-627)SPR051894416 (SPR)s10722-023-01542-7-e DE-627 ger DE-627 rakwb eng Baloch, Faheem Shehzad verfasserin (orcid)0000-0002-7470-0080 aut iPBS-retrotransposons variations: DNA fingerprinting and the evaluation of genetic diversity and population structure in international cowpea germplasm 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Cowpea belongs to the fabaceae family, originated in Africa, and grown in subtropical and tropical areas worldwide. Cowpea seeds contain high nutritional contents and play a vital role in nutritional feed for animals and humans in many countries. The present investigation aimed to check the genetic variations among cowpea germplasm collected from 6 countries using iPBS-retrotransposons markers system. Twelve highly polymorphic iPBS-retrotransposons primers were used for PCR amplification. These primers generated 200 total bands of which 188 were highly polymorphic and 94.30% polymorphism was observed. Mean values of genetic diversity indices i.e., Shannon’s information index (I = 0.452), effective number of alleles (ne = 1.501), overall gene diversity (ht = 0.236), gene diversity (h = 0.298), polymorphism information content (PIC = 0.308), and average genetic distance 0.61 confirmed a great level of genetic variations in studied germplasm. Analysis of molecular variance revealed 96% variation within the population. The STRUCTURE analysis divided germplasm into 2 populations according to geographically. The Neighbor-joining tree and Principle coordinate analysis (PCoA) divided the germplasm into 3 groups and the grouping was mostly aline with STRUCTURE-based clustering. This study revealed high genetic variability in cowpea germplasm and the high values of diversity indices revealed the utility and efficiency of iPBS-retrotransposons marker system. The PCoA, Neighbor-joining tree and STRUCTURE analysis separated the accessions geographically. Analysis of molecular variance revealed 94% genetic variation within a population and Ghana 1 and Turkey 4 had the greatest genetic distance and could be recommended for breeding activities. iPBS-retrotransposons (dpeaa)DE-He213 Cowpea germplasm (dpeaa)DE-He213 Fabaceae (dpeaa)DE-He213 Population structure (dpeaa)DE-He213 Genetic diversity (dpeaa)DE-He213 Altaf, Muhammad Tanveer aut Bedir, Mehmet aut Nadeem, Muhammad Azhar aut Tatar, Muhammed aut Karaköy, Tolga aut Aasim, Muhammad aut Enthalten in Genetic resources and crop evolution Dordrecht [u.a.] : Springer Science + Business Media B.V, 1953 70(2023), 6 vom: 03. Feb., Seite 1867-1877 (DE-627)320529029 (DE-600)2015535-9 1573-5109 nnns volume:70 year:2023 number:6 day:03 month:02 pages:1867-1877 https://dx.doi.org/10.1007/s10722-023-01542-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 70 2023 6 03 02 1867-1877 |
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10.1007/s10722-023-01542-7 doi (DE-627)SPR051894416 (SPR)s10722-023-01542-7-e DE-627 ger DE-627 rakwb eng Baloch, Faheem Shehzad verfasserin (orcid)0000-0002-7470-0080 aut iPBS-retrotransposons variations: DNA fingerprinting and the evaluation of genetic diversity and population structure in international cowpea germplasm 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Cowpea belongs to the fabaceae family, originated in Africa, and grown in subtropical and tropical areas worldwide. Cowpea seeds contain high nutritional contents and play a vital role in nutritional feed for animals and humans in many countries. The present investigation aimed to check the genetic variations among cowpea germplasm collected from 6 countries using iPBS-retrotransposons markers system. Twelve highly polymorphic iPBS-retrotransposons primers were used for PCR amplification. These primers generated 200 total bands of which 188 were highly polymorphic and 94.30% polymorphism was observed. Mean values of genetic diversity indices i.e., Shannon’s information index (I = 0.452), effective number of alleles (ne = 1.501), overall gene diversity (ht = 0.236), gene diversity (h = 0.298), polymorphism information content (PIC = 0.308), and average genetic distance 0.61 confirmed a great level of genetic variations in studied germplasm. Analysis of molecular variance revealed 96% variation within the population. The STRUCTURE analysis divided germplasm into 2 populations according to geographically. The Neighbor-joining tree and Principle coordinate analysis (PCoA) divided the germplasm into 3 groups and the grouping was mostly aline with STRUCTURE-based clustering. This study revealed high genetic variability in cowpea germplasm and the high values of diversity indices revealed the utility and efficiency of iPBS-retrotransposons marker system. The PCoA, Neighbor-joining tree and STRUCTURE analysis separated the accessions geographically. Analysis of molecular variance revealed 94% genetic variation within a population and Ghana 1 and Turkey 4 had the greatest genetic distance and could be recommended for breeding activities. iPBS-retrotransposons (dpeaa)DE-He213 Cowpea germplasm (dpeaa)DE-He213 Fabaceae (dpeaa)DE-He213 Population structure (dpeaa)DE-He213 Genetic diversity (dpeaa)DE-He213 Altaf, Muhammad Tanveer aut Bedir, Mehmet aut Nadeem, Muhammad Azhar aut Tatar, Muhammed aut Karaköy, Tolga aut Aasim, Muhammad aut Enthalten in Genetic resources and crop evolution Dordrecht [u.a.] : Springer Science + Business Media B.V, 1953 70(2023), 6 vom: 03. Feb., Seite 1867-1877 (DE-627)320529029 (DE-600)2015535-9 1573-5109 nnns volume:70 year:2023 number:6 day:03 month:02 pages:1867-1877 https://dx.doi.org/10.1007/s10722-023-01542-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 70 2023 6 03 02 1867-1877 |
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10.1007/s10722-023-01542-7 doi (DE-627)SPR051894416 (SPR)s10722-023-01542-7-e DE-627 ger DE-627 rakwb eng Baloch, Faheem Shehzad verfasserin (orcid)0000-0002-7470-0080 aut iPBS-retrotransposons variations: DNA fingerprinting and the evaluation of genetic diversity and population structure in international cowpea germplasm 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Cowpea belongs to the fabaceae family, originated in Africa, and grown in subtropical and tropical areas worldwide. Cowpea seeds contain high nutritional contents and play a vital role in nutritional feed for animals and humans in many countries. The present investigation aimed to check the genetic variations among cowpea germplasm collected from 6 countries using iPBS-retrotransposons markers system. Twelve highly polymorphic iPBS-retrotransposons primers were used for PCR amplification. These primers generated 200 total bands of which 188 were highly polymorphic and 94.30% polymorphism was observed. Mean values of genetic diversity indices i.e., Shannon’s information index (I = 0.452), effective number of alleles (ne = 1.501), overall gene diversity (ht = 0.236), gene diversity (h = 0.298), polymorphism information content (PIC = 0.308), and average genetic distance 0.61 confirmed a great level of genetic variations in studied germplasm. Analysis of molecular variance revealed 96% variation within the population. The STRUCTURE analysis divided germplasm into 2 populations according to geographically. The Neighbor-joining tree and Principle coordinate analysis (PCoA) divided the germplasm into 3 groups and the grouping was mostly aline with STRUCTURE-based clustering. This study revealed high genetic variability in cowpea germplasm and the high values of diversity indices revealed the utility and efficiency of iPBS-retrotransposons marker system. The PCoA, Neighbor-joining tree and STRUCTURE analysis separated the accessions geographically. Analysis of molecular variance revealed 94% genetic variation within a population and Ghana 1 and Turkey 4 had the greatest genetic distance and could be recommended for breeding activities. iPBS-retrotransposons (dpeaa)DE-He213 Cowpea germplasm (dpeaa)DE-He213 Fabaceae (dpeaa)DE-He213 Population structure (dpeaa)DE-He213 Genetic diversity (dpeaa)DE-He213 Altaf, Muhammad Tanveer aut Bedir, Mehmet aut Nadeem, Muhammad Azhar aut Tatar, Muhammed aut Karaköy, Tolga aut Aasim, Muhammad aut Enthalten in Genetic resources and crop evolution Dordrecht [u.a.] : Springer Science + Business Media B.V, 1953 70(2023), 6 vom: 03. Feb., Seite 1867-1877 (DE-627)320529029 (DE-600)2015535-9 1573-5109 nnns volume:70 year:2023 number:6 day:03 month:02 pages:1867-1877 https://dx.doi.org/10.1007/s10722-023-01542-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 70 2023 6 03 02 1867-1877 |
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Baloch, Faheem Shehzad @@aut@@ Altaf, Muhammad Tanveer @@aut@@ Bedir, Mehmet @@aut@@ Nadeem, Muhammad Azhar @@aut@@ Tatar, Muhammed @@aut@@ Karaköy, Tolga @@aut@@ Aasim, Muhammad @@aut@@ |
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Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Cowpea belongs to the fabaceae family, originated in Africa, and grown in subtropical and tropical areas worldwide. Cowpea seeds contain high nutritional contents and play a vital role in nutritional feed for animals and humans in many countries. The present investigation aimed to check the genetic variations among cowpea germplasm collected from 6 countries using iPBS-retrotransposons markers system. Twelve highly polymorphic iPBS-retrotransposons primers were used for PCR amplification. These primers generated 200 total bands of which 188 were highly polymorphic and 94.30% polymorphism was observed. Mean values of genetic diversity indices i.e., Shannon’s information index (I = 0.452), effective number of alleles (ne = 1.501), overall gene diversity (ht = 0.236), gene diversity (h = 0.298), polymorphism information content (PIC = 0.308), and average genetic distance 0.61 confirmed a great level of genetic variations in studied germplasm. Analysis of molecular variance revealed 96% variation within the population. The STRUCTURE analysis divided germplasm into 2 populations according to geographically. The Neighbor-joining tree and Principle coordinate analysis (PCoA) divided the germplasm into 3 groups and the grouping was mostly aline with STRUCTURE-based clustering. This study revealed high genetic variability in cowpea germplasm and the high values of diversity indices revealed the utility and efficiency of iPBS-retrotransposons marker system. The PCoA, Neighbor-joining tree and STRUCTURE analysis separated the accessions geographically. 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|
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Baloch, Faheem Shehzad |
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Baloch, Faheem Shehzad misc iPBS-retrotransposons misc Cowpea germplasm misc Fabaceae misc Population structure misc Genetic diversity iPBS-retrotransposons variations: DNA fingerprinting and the evaluation of genetic diversity and population structure in international cowpea germplasm |
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iPBS-retrotransposons variations: DNA fingerprinting and the evaluation of genetic diversity and population structure in international cowpea germplasm iPBS-retrotransposons (dpeaa)DE-He213 Cowpea germplasm (dpeaa)DE-He213 Fabaceae (dpeaa)DE-He213 Population structure (dpeaa)DE-He213 Genetic diversity (dpeaa)DE-He213 |
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misc iPBS-retrotransposons misc Cowpea germplasm misc Fabaceae misc Population structure misc Genetic diversity |
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misc iPBS-retrotransposons misc Cowpea germplasm misc Fabaceae misc Population structure misc Genetic diversity |
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iPBS-retrotransposons variations: DNA fingerprinting and the evaluation of genetic diversity and population structure in international cowpea germplasm |
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iPBS-retrotransposons variations: DNA fingerprinting and the evaluation of genetic diversity and population structure in international cowpea germplasm |
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Baloch, Faheem Shehzad |
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Baloch, Faheem Shehzad Altaf, Muhammad Tanveer Bedir, Mehmet Nadeem, Muhammad Azhar Tatar, Muhammed Karaköy, Tolga Aasim, Muhammad |
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ipbs-retrotransposons variations: dna fingerprinting and the evaluation of genetic diversity and population structure in international cowpea germplasm |
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iPBS-retrotransposons variations: DNA fingerprinting and the evaluation of genetic diversity and population structure in international cowpea germplasm |
| abstract |
Abstract Cowpea belongs to the fabaceae family, originated in Africa, and grown in subtropical and tropical areas worldwide. Cowpea seeds contain high nutritional contents and play a vital role in nutritional feed for animals and humans in many countries. The present investigation aimed to check the genetic variations among cowpea germplasm collected from 6 countries using iPBS-retrotransposons markers system. Twelve highly polymorphic iPBS-retrotransposons primers were used for PCR amplification. These primers generated 200 total bands of which 188 were highly polymorphic and 94.30% polymorphism was observed. Mean values of genetic diversity indices i.e., Shannon’s information index (I = 0.452), effective number of alleles (ne = 1.501), overall gene diversity (ht = 0.236), gene diversity (h = 0.298), polymorphism information content (PIC = 0.308), and average genetic distance 0.61 confirmed a great level of genetic variations in studied germplasm. Analysis of molecular variance revealed 96% variation within the population. The STRUCTURE analysis divided germplasm into 2 populations according to geographically. The Neighbor-joining tree and Principle coordinate analysis (PCoA) divided the germplasm into 3 groups and the grouping was mostly aline with STRUCTURE-based clustering. This study revealed high genetic variability in cowpea germplasm and the high values of diversity indices revealed the utility and efficiency of iPBS-retrotransposons marker system. The PCoA, Neighbor-joining tree and STRUCTURE analysis separated the accessions geographically. Analysis of molecular variance revealed 94% genetic variation within a population and Ghana 1 and Turkey 4 had the greatest genetic distance and could be recommended for breeding activities. © The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
| abstractGer |
Abstract Cowpea belongs to the fabaceae family, originated in Africa, and grown in subtropical and tropical areas worldwide. Cowpea seeds contain high nutritional contents and play a vital role in nutritional feed for animals and humans in many countries. The present investigation aimed to check the genetic variations among cowpea germplasm collected from 6 countries using iPBS-retrotransposons markers system. Twelve highly polymorphic iPBS-retrotransposons primers were used for PCR amplification. These primers generated 200 total bands of which 188 were highly polymorphic and 94.30% polymorphism was observed. Mean values of genetic diversity indices i.e., Shannon’s information index (I = 0.452), effective number of alleles (ne = 1.501), overall gene diversity (ht = 0.236), gene diversity (h = 0.298), polymorphism information content (PIC = 0.308), and average genetic distance 0.61 confirmed a great level of genetic variations in studied germplasm. Analysis of molecular variance revealed 96% variation within the population. The STRUCTURE analysis divided germplasm into 2 populations according to geographically. The Neighbor-joining tree and Principle coordinate analysis (PCoA) divided the germplasm into 3 groups and the grouping was mostly aline with STRUCTURE-based clustering. This study revealed high genetic variability in cowpea germplasm and the high values of diversity indices revealed the utility and efficiency of iPBS-retrotransposons marker system. The PCoA, Neighbor-joining tree and STRUCTURE analysis separated the accessions geographically. Analysis of molecular variance revealed 94% genetic variation within a population and Ghana 1 and Turkey 4 had the greatest genetic distance and could be recommended for breeding activities. © The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
| abstract_unstemmed |
Abstract Cowpea belongs to the fabaceae family, originated in Africa, and grown in subtropical and tropical areas worldwide. Cowpea seeds contain high nutritional contents and play a vital role in nutritional feed for animals and humans in many countries. The present investigation aimed to check the genetic variations among cowpea germplasm collected from 6 countries using iPBS-retrotransposons markers system. Twelve highly polymorphic iPBS-retrotransposons primers were used for PCR amplification. These primers generated 200 total bands of which 188 were highly polymorphic and 94.30% polymorphism was observed. Mean values of genetic diversity indices i.e., Shannon’s information index (I = 0.452), effective number of alleles (ne = 1.501), overall gene diversity (ht = 0.236), gene diversity (h = 0.298), polymorphism information content (PIC = 0.308), and average genetic distance 0.61 confirmed a great level of genetic variations in studied germplasm. Analysis of molecular variance revealed 96% variation within the population. The STRUCTURE analysis divided germplasm into 2 populations according to geographically. The Neighbor-joining tree and Principle coordinate analysis (PCoA) divided the germplasm into 3 groups and the grouping was mostly aline with STRUCTURE-based clustering. This study revealed high genetic variability in cowpea germplasm and the high values of diversity indices revealed the utility and efficiency of iPBS-retrotransposons marker system. The PCoA, Neighbor-joining tree and STRUCTURE analysis separated the accessions geographically. Analysis of molecular variance revealed 94% genetic variation within a population and Ghana 1 and Turkey 4 had the greatest genetic distance and could be recommended for breeding activities. © The Author(s), under exclusive licence to Springer Nature B.V. 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| title_short |
iPBS-retrotransposons variations: DNA fingerprinting and the evaluation of genetic diversity and population structure in international cowpea germplasm |
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https://dx.doi.org/10.1007/s10722-023-01542-7 |
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Altaf, Muhammad Tanveer Bedir, Mehmet Nadeem, Muhammad Azhar Tatar, Muhammed Karaköy, Tolga Aasim, Muhammad |
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Altaf, Muhammad Tanveer Bedir, Mehmet Nadeem, Muhammad Azhar Tatar, Muhammed Karaköy, Tolga Aasim, Muhammad |
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2024-07-04T00:18:55.315Z |
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| score |
7.402128 |