PCR–RFLP analysis for detecting potential QoI and CAA fungicide resistance in onion and lettuce downy mildews
Abstract Downy mildew of onion and lettuce, caused by Peronospora destructor and Bremia lactucae, respectively, is distributed worldwide. Control of these serious diseases largely relies on oomycete fungicides including quinone-outside inhibitors (QoIs) and carboxylic acid amides (CAAs), both of whi...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Ishii, Hideo [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2023 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s), under exclusive licence to Deutsche Phytomedizinische Gesellschaft 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| Übergeordnetes Werk: |
Enthalten in: Journal of plant diseases and protection - Berlin : Springer, 2006, 130(2023), 5 vom: 18. März, Seite 985-990 |
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| Übergeordnetes Werk: |
volume:130 ; year:2023 ; number:5 ; day:18 ; month:03 ; pages:985-990 |
| Links: |
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| DOI / URN: |
10.1007/s41348-023-00732-w |
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| Katalog-ID: |
SPR052725588 |
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| 100 | 1 | |a Ishii, Hideo |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a PCR–RFLP analysis for detecting potential QoI and CAA fungicide resistance in onion and lettuce downy mildews |
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| 520 | |a Abstract Downy mildew of onion and lettuce, caused by Peronospora destructor and Bremia lactucae, respectively, is distributed worldwide. Control of these serious diseases largely relies on oomycete fungicides including quinone-outside inhibitors (QoIs) and carboxylic acid amides (CAAs), both of which bear the risk of resistance development in pathogens. Resistance to QoI and CAA fungicides has not been confirmed in field isolates of onion and lettuce downy mildews to date, and monitoring using biological methods is challenging as both pathogens are obligate biotrophs and cannot be grown in agar culture. In this study, the RFLP-method using PCR-amplified gene fragments encoding the fungicide targets cytochrome b (cytb) and cellulose synthase 3 (CesA3) has been developed for detecting potential QoI and CAA resistance in P. destructor and B. lactucae, as this is likely to develop in the future. This method will make it possible to diagnose resistance prior to efficacy reduction of fungicides contributing to sustainable disease control. | ||
| 650 | 4 | |a CAA fungicides |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Fungicide resistance |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a QoI fungicides |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Stammler, Gerd |4 aut | |
| 700 | 1 | |a Yamabe, Shuhei |4 aut | |
| 700 | 1 | |a Tashiro, Nobuya |4 aut | |
| 700 | 1 | |a Yamaoka, Yuichi |4 aut | |
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| 912 | |a GBV_ILN_60 | ||
| 912 | |a GBV_ILN_62 | ||
| 912 | |a GBV_ILN_63 | ||
| 912 | |a GBV_ILN_65 | ||
| 912 | |a GBV_ILN_69 | ||
| 912 | |a GBV_ILN_70 | ||
| 912 | |a GBV_ILN_73 | ||
| 912 | |a GBV_ILN_74 | ||
| 912 | |a GBV_ILN_90 | ||
| 912 | |a GBV_ILN_95 | ||
| 912 | |a GBV_ILN_100 | ||
| 912 | |a GBV_ILN_105 | ||
| 912 | |a GBV_ILN_110 | ||
| 912 | |a GBV_ILN_120 | ||
| 912 | |a GBV_ILN_138 | ||
| 912 | |a GBV_ILN_150 | ||
| 912 | |a GBV_ILN_151 | ||
| 912 | |a GBV_ILN_152 | ||
| 912 | |a GBV_ILN_161 | ||
| 912 | |a GBV_ILN_170 | ||
| 912 | |a GBV_ILN_171 | ||
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| 912 | |a GBV_ILN_213 | ||
| 912 | |a GBV_ILN_224 | ||
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| 912 | |a GBV_ILN_374 | ||
| 912 | |a GBV_ILN_602 | ||
| 912 | |a GBV_ILN_636 | ||
| 912 | |a GBV_ILN_702 | ||
| 912 | |a GBV_ILN_2001 | ||
| 912 | |a GBV_ILN_2003 | ||
| 912 | |a GBV_ILN_2004 | ||
| 912 | |a GBV_ILN_2005 | ||
| 912 | |a GBV_ILN_2006 | ||
| 912 | |a GBV_ILN_2007 | ||
| 912 | |a GBV_ILN_2008 | ||
| 912 | |a GBV_ILN_2009 | ||
| 912 | |a GBV_ILN_2010 | ||
| 912 | |a GBV_ILN_2011 | ||
| 912 | |a GBV_ILN_2014 | ||
| 912 | |a GBV_ILN_2015 | ||
| 912 | |a GBV_ILN_2018 | ||
| 912 | |a GBV_ILN_2020 | ||
| 912 | |a GBV_ILN_2021 | ||
| 912 | |a GBV_ILN_2025 | ||
| 912 | |a GBV_ILN_2026 | ||
| 912 | |a GBV_ILN_2027 | ||
| 912 | |a GBV_ILN_2031 | ||
| 912 | |a GBV_ILN_2034 | ||
| 912 | |a GBV_ILN_2037 | ||
| 912 | |a GBV_ILN_2038 | ||
| 912 | |a GBV_ILN_2039 | ||
| 912 | |a GBV_ILN_2044 | ||
| 912 | |a GBV_ILN_2048 | ||
| 912 | |a GBV_ILN_2049 | ||
| 912 | |a GBV_ILN_2050 | ||
| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2056 | ||
| 912 | |a GBV_ILN_2057 | ||
| 912 | |a GBV_ILN_2059 | ||
| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2064 | ||
| 912 | |a GBV_ILN_2065 | ||
| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
| 912 | |a GBV_ILN_2108 | ||
| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2111 | ||
| 912 | |a GBV_ILN_2112 | ||
| 912 | |a GBV_ILN_2113 | ||
| 912 | |a GBV_ILN_2118 | ||
| 912 | |a GBV_ILN_2122 | ||
| 912 | |a GBV_ILN_2129 | ||
| 912 | |a GBV_ILN_2143 | ||
| 912 | |a GBV_ILN_2144 | ||
| 912 | |a GBV_ILN_2147 | ||
| 912 | |a GBV_ILN_2148 | ||
| 912 | |a GBV_ILN_2152 | ||
| 912 | |a GBV_ILN_2153 | ||
| 912 | |a GBV_ILN_2188 | ||
| 912 | |a GBV_ILN_2190 | ||
| 912 | |a GBV_ILN_2232 | ||
| 912 | |a GBV_ILN_2336 | ||
| 912 | |a GBV_ILN_2446 | ||
| 912 | |a GBV_ILN_2470 | ||
| 912 | |a GBV_ILN_2472 | ||
| 912 | |a GBV_ILN_2507 | ||
| 912 | |a GBV_ILN_2522 | ||
| 912 | |a GBV_ILN_2548 | ||
| 912 | |a GBV_ILN_2946 | ||
| 912 | |a GBV_ILN_2949 | ||
| 912 | |a GBV_ILN_2951 | ||
| 912 | |a GBV_ILN_4012 | ||
| 912 | |a GBV_ILN_4035 | ||
| 912 | |a GBV_ILN_4037 | ||
| 912 | |a GBV_ILN_4046 | ||
| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4125 | ||
| 912 | |a GBV_ILN_4126 | ||
| 912 | |a GBV_ILN_4242 | ||
| 912 | |a GBV_ILN_4246 | ||
| 912 | |a GBV_ILN_4249 | ||
| 912 | |a GBV_ILN_4251 | ||
| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4306 | ||
| 912 | |a GBV_ILN_4307 | ||
| 912 | |a GBV_ILN_4313 | ||
| 912 | |a GBV_ILN_4322 | ||
| 912 | |a GBV_ILN_4323 | ||
| 912 | |a GBV_ILN_4324 | ||
| 912 | |a GBV_ILN_4325 | ||
| 912 | |a GBV_ILN_4326 | ||
| 912 | |a GBV_ILN_4328 | ||
| 912 | |a GBV_ILN_4333 | ||
| 912 | |a GBV_ILN_4334 | ||
| 912 | |a GBV_ILN_4335 | ||
| 912 | |a GBV_ILN_4336 | ||
| 912 | |a GBV_ILN_4338 | ||
| 912 | |a GBV_ILN_4346 | ||
| 912 | |a GBV_ILN_4393 | ||
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10.1007/s41348-023-00732-w doi (DE-627)SPR052725588 (SPR)s41348-023-00732-w-e DE-627 ger DE-627 rakwb eng Ishii, Hideo verfasserin aut PCR–RFLP analysis for detecting potential QoI and CAA fungicide resistance in onion and lettuce downy mildews 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Deutsche Phytomedizinische Gesellschaft 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Downy mildew of onion and lettuce, caused by Peronospora destructor and Bremia lactucae, respectively, is distributed worldwide. Control of these serious diseases largely relies on oomycete fungicides including quinone-outside inhibitors (QoIs) and carboxylic acid amides (CAAs), both of which bear the risk of resistance development in pathogens. Resistance to QoI and CAA fungicides has not been confirmed in field isolates of onion and lettuce downy mildews to date, and monitoring using biological methods is challenging as both pathogens are obligate biotrophs and cannot be grown in agar culture. In this study, the RFLP-method using PCR-amplified gene fragments encoding the fungicide targets cytochrome b (cytb) and cellulose synthase 3 (CesA3) has been developed for detecting potential QoI and CAA resistance in P. destructor and B. lactucae, as this is likely to develop in the future. This method will make it possible to diagnose resistance prior to efficacy reduction of fungicides contributing to sustainable disease control. CAA fungicides (dpeaa)DE-He213 Fungicide resistance (dpeaa)DE-He213 QoI fungicides (dpeaa)DE-He213 Stammler, Gerd aut Yamabe, Shuhei aut Tashiro, Nobuya aut Yamaoka, Yuichi aut Enthalten in Journal of plant diseases and protection Berlin : Springer, 2006 130(2023), 5 vom: 18. März, Seite 985-990 (DE-627)508335310 (DE-600)2224048-2 1861-3837 nnns volume:130 year:2023 number:5 day:18 month:03 pages:985-990 https://dx.doi.org/10.1007/s41348-023-00732-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 130 2023 5 18 03 985-990 |
| spelling |
10.1007/s41348-023-00732-w doi (DE-627)SPR052725588 (SPR)s41348-023-00732-w-e DE-627 ger DE-627 rakwb eng Ishii, Hideo verfasserin aut PCR–RFLP analysis for detecting potential QoI and CAA fungicide resistance in onion and lettuce downy mildews 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Deutsche Phytomedizinische Gesellschaft 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Downy mildew of onion and lettuce, caused by Peronospora destructor and Bremia lactucae, respectively, is distributed worldwide. Control of these serious diseases largely relies on oomycete fungicides including quinone-outside inhibitors (QoIs) and carboxylic acid amides (CAAs), both of which bear the risk of resistance development in pathogens. Resistance to QoI and CAA fungicides has not been confirmed in field isolates of onion and lettuce downy mildews to date, and monitoring using biological methods is challenging as both pathogens are obligate biotrophs and cannot be grown in agar culture. In this study, the RFLP-method using PCR-amplified gene fragments encoding the fungicide targets cytochrome b (cytb) and cellulose synthase 3 (CesA3) has been developed for detecting potential QoI and CAA resistance in P. destructor and B. lactucae, as this is likely to develop in the future. This method will make it possible to diagnose resistance prior to efficacy reduction of fungicides contributing to sustainable disease control. CAA fungicides (dpeaa)DE-He213 Fungicide resistance (dpeaa)DE-He213 QoI fungicides (dpeaa)DE-He213 Stammler, Gerd aut Yamabe, Shuhei aut Tashiro, Nobuya aut Yamaoka, Yuichi aut Enthalten in Journal of plant diseases and protection Berlin : Springer, 2006 130(2023), 5 vom: 18. März, Seite 985-990 (DE-627)508335310 (DE-600)2224048-2 1861-3837 nnns volume:130 year:2023 number:5 day:18 month:03 pages:985-990 https://dx.doi.org/10.1007/s41348-023-00732-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 130 2023 5 18 03 985-990 |
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10.1007/s41348-023-00732-w doi (DE-627)SPR052725588 (SPR)s41348-023-00732-w-e DE-627 ger DE-627 rakwb eng Ishii, Hideo verfasserin aut PCR–RFLP analysis for detecting potential QoI and CAA fungicide resistance in onion and lettuce downy mildews 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Deutsche Phytomedizinische Gesellschaft 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Downy mildew of onion and lettuce, caused by Peronospora destructor and Bremia lactucae, respectively, is distributed worldwide. Control of these serious diseases largely relies on oomycete fungicides including quinone-outside inhibitors (QoIs) and carboxylic acid amides (CAAs), both of which bear the risk of resistance development in pathogens. Resistance to QoI and CAA fungicides has not been confirmed in field isolates of onion and lettuce downy mildews to date, and monitoring using biological methods is challenging as both pathogens are obligate biotrophs and cannot be grown in agar culture. In this study, the RFLP-method using PCR-amplified gene fragments encoding the fungicide targets cytochrome b (cytb) and cellulose synthase 3 (CesA3) has been developed for detecting potential QoI and CAA resistance in P. destructor and B. lactucae, as this is likely to develop in the future. This method will make it possible to diagnose resistance prior to efficacy reduction of fungicides contributing to sustainable disease control. CAA fungicides (dpeaa)DE-He213 Fungicide resistance (dpeaa)DE-He213 QoI fungicides (dpeaa)DE-He213 Stammler, Gerd aut Yamabe, Shuhei aut Tashiro, Nobuya aut Yamaoka, Yuichi aut Enthalten in Journal of plant diseases and protection Berlin : Springer, 2006 130(2023), 5 vom: 18. März, Seite 985-990 (DE-627)508335310 (DE-600)2224048-2 1861-3837 nnns volume:130 year:2023 number:5 day:18 month:03 pages:985-990 https://dx.doi.org/10.1007/s41348-023-00732-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 130 2023 5 18 03 985-990 |
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10.1007/s41348-023-00732-w doi (DE-627)SPR052725588 (SPR)s41348-023-00732-w-e DE-627 ger DE-627 rakwb eng Ishii, Hideo verfasserin aut PCR–RFLP analysis for detecting potential QoI and CAA fungicide resistance in onion and lettuce downy mildews 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Deutsche Phytomedizinische Gesellschaft 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Downy mildew of onion and lettuce, caused by Peronospora destructor and Bremia lactucae, respectively, is distributed worldwide. Control of these serious diseases largely relies on oomycete fungicides including quinone-outside inhibitors (QoIs) and carboxylic acid amides (CAAs), both of which bear the risk of resistance development in pathogens. Resistance to QoI and CAA fungicides has not been confirmed in field isolates of onion and lettuce downy mildews to date, and monitoring using biological methods is challenging as both pathogens are obligate biotrophs and cannot be grown in agar culture. In this study, the RFLP-method using PCR-amplified gene fragments encoding the fungicide targets cytochrome b (cytb) and cellulose synthase 3 (CesA3) has been developed for detecting potential QoI and CAA resistance in P. destructor and B. lactucae, as this is likely to develop in the future. This method will make it possible to diagnose resistance prior to efficacy reduction of fungicides contributing to sustainable disease control. CAA fungicides (dpeaa)DE-He213 Fungicide resistance (dpeaa)DE-He213 QoI fungicides (dpeaa)DE-He213 Stammler, Gerd aut Yamabe, Shuhei aut Tashiro, Nobuya aut Yamaoka, Yuichi aut Enthalten in Journal of plant diseases and protection Berlin : Springer, 2006 130(2023), 5 vom: 18. März, Seite 985-990 (DE-627)508335310 (DE-600)2224048-2 1861-3837 nnns volume:130 year:2023 number:5 day:18 month:03 pages:985-990 https://dx.doi.org/10.1007/s41348-023-00732-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 130 2023 5 18 03 985-990 |
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10.1007/s41348-023-00732-w doi (DE-627)SPR052725588 (SPR)s41348-023-00732-w-e DE-627 ger DE-627 rakwb eng Ishii, Hideo verfasserin aut PCR–RFLP analysis for detecting potential QoI and CAA fungicide resistance in onion and lettuce downy mildews 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Deutsche Phytomedizinische Gesellschaft 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Abstract Downy mildew of onion and lettuce, caused by Peronospora destructor and Bremia lactucae, respectively, is distributed worldwide. Control of these serious diseases largely relies on oomycete fungicides including quinone-outside inhibitors (QoIs) and carboxylic acid amides (CAAs), both of which bear the risk of resistance development in pathogens. Resistance to QoI and CAA fungicides has not been confirmed in field isolates of onion and lettuce downy mildews to date, and monitoring using biological methods is challenging as both pathogens are obligate biotrophs and cannot be grown in agar culture. In this study, the RFLP-method using PCR-amplified gene fragments encoding the fungicide targets cytochrome b (cytb) and cellulose synthase 3 (CesA3) has been developed for detecting potential QoI and CAA resistance in P. destructor and B. lactucae, as this is likely to develop in the future. This method will make it possible to diagnose resistance prior to efficacy reduction of fungicides contributing to sustainable disease control. CAA fungicides (dpeaa)DE-He213 Fungicide resistance (dpeaa)DE-He213 QoI fungicides (dpeaa)DE-He213 Stammler, Gerd aut Yamabe, Shuhei aut Tashiro, Nobuya aut Yamaoka, Yuichi aut Enthalten in Journal of plant diseases and protection Berlin : Springer, 2006 130(2023), 5 vom: 18. März, Seite 985-990 (DE-627)508335310 (DE-600)2224048-2 1861-3837 nnns volume:130 year:2023 number:5 day:18 month:03 pages:985-990 https://dx.doi.org/10.1007/s41348-023-00732-w lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2946 GBV_ILN_2949 GBV_ILN_2951 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4393 GBV_ILN_4700 AR 130 2023 5 18 03 985-990 |
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Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Downy mildew of onion and lettuce, caused by Peronospora destructor and Bremia lactucae, respectively, is distributed worldwide. Control of these serious diseases largely relies on oomycete fungicides including quinone-outside inhibitors (QoIs) and carboxylic acid amides (CAAs), both of which bear the risk of resistance development in pathogens. Resistance to QoI and CAA fungicides has not been confirmed in field isolates of onion and lettuce downy mildews to date, and monitoring using biological methods is challenging as both pathogens are obligate biotrophs and cannot be grown in agar culture. In this study, the RFLP-method using PCR-amplified gene fragments encoding the fungicide targets cytochrome b (cytb) and cellulose synthase 3 (CesA3) has been developed for detecting potential QoI and CAA resistance in P. destructor and B. lactucae, as this is likely to develop in the future. 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Ishii, Hideo |
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Ishii, Hideo misc CAA fungicides misc Fungicide resistance misc QoI fungicides PCR–RFLP analysis for detecting potential QoI and CAA fungicide resistance in onion and lettuce downy mildews |
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PCR–RFLP analysis for detecting potential QoI and CAA fungicide resistance in onion and lettuce downy mildews CAA fungicides (dpeaa)DE-He213 Fungicide resistance (dpeaa)DE-He213 QoI fungicides (dpeaa)DE-He213 |
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PCR–RFLP analysis for detecting potential QoI and CAA fungicide resistance in onion and lettuce downy mildews |
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pcr–rflp analysis for detecting potential qoi and caa fungicide resistance in onion and lettuce downy mildews |
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PCR–RFLP analysis for detecting potential QoI and CAA fungicide resistance in onion and lettuce downy mildews |
| abstract |
Abstract Downy mildew of onion and lettuce, caused by Peronospora destructor and Bremia lactucae, respectively, is distributed worldwide. Control of these serious diseases largely relies on oomycete fungicides including quinone-outside inhibitors (QoIs) and carboxylic acid amides (CAAs), both of which bear the risk of resistance development in pathogens. Resistance to QoI and CAA fungicides has not been confirmed in field isolates of onion and lettuce downy mildews to date, and monitoring using biological methods is challenging as both pathogens are obligate biotrophs and cannot be grown in agar culture. In this study, the RFLP-method using PCR-amplified gene fragments encoding the fungicide targets cytochrome b (cytb) and cellulose synthase 3 (CesA3) has been developed for detecting potential QoI and CAA resistance in P. destructor and B. lactucae, as this is likely to develop in the future. This method will make it possible to diagnose resistance prior to efficacy reduction of fungicides contributing to sustainable disease control. © The Author(s), under exclusive licence to Deutsche Phytomedizinische Gesellschaft 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
| abstractGer |
Abstract Downy mildew of onion and lettuce, caused by Peronospora destructor and Bremia lactucae, respectively, is distributed worldwide. Control of these serious diseases largely relies on oomycete fungicides including quinone-outside inhibitors (QoIs) and carboxylic acid amides (CAAs), both of which bear the risk of resistance development in pathogens. Resistance to QoI and CAA fungicides has not been confirmed in field isolates of onion and lettuce downy mildews to date, and monitoring using biological methods is challenging as both pathogens are obligate biotrophs and cannot be grown in agar culture. In this study, the RFLP-method using PCR-amplified gene fragments encoding the fungicide targets cytochrome b (cytb) and cellulose synthase 3 (CesA3) has been developed for detecting potential QoI and CAA resistance in P. destructor and B. lactucae, as this is likely to develop in the future. This method will make it possible to diagnose resistance prior to efficacy reduction of fungicides contributing to sustainable disease control. © The Author(s), under exclusive licence to Deutsche Phytomedizinische Gesellschaft 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
| abstract_unstemmed |
Abstract Downy mildew of onion and lettuce, caused by Peronospora destructor and Bremia lactucae, respectively, is distributed worldwide. Control of these serious diseases largely relies on oomycete fungicides including quinone-outside inhibitors (QoIs) and carboxylic acid amides (CAAs), both of which bear the risk of resistance development in pathogens. Resistance to QoI and CAA fungicides has not been confirmed in field isolates of onion and lettuce downy mildews to date, and monitoring using biological methods is challenging as both pathogens are obligate biotrophs and cannot be grown in agar culture. In this study, the RFLP-method using PCR-amplified gene fragments encoding the fungicide targets cytochrome b (cytb) and cellulose synthase 3 (CesA3) has been developed for detecting potential QoI and CAA resistance in P. destructor and B. lactucae, as this is likely to develop in the future. This method will make it possible to diagnose resistance prior to efficacy reduction of fungicides contributing to sustainable disease control. © The Author(s), under exclusive licence to Deutsche Phytomedizinische Gesellschaft 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| container_issue |
5 |
| title_short |
PCR–RFLP analysis for detecting potential QoI and CAA fungicide resistance in onion and lettuce downy mildews |
| url |
https://dx.doi.org/10.1007/s41348-023-00732-w |
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| author2 |
Stammler, Gerd Yamabe, Shuhei Tashiro, Nobuya Yamaoka, Yuichi |
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Stammler, Gerd Yamabe, Shuhei Tashiro, Nobuya Yamaoka, Yuichi |
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| doi_str |
10.1007/s41348-023-00732-w |
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2024-07-03T14:20:24.908Z |
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| score |
7.3992662 |