Effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells
Background 3D cancer stem cell (CSC) cultures are widely used as in vitro tumor models. In this study, we determined the effects of enriching HCT116 tumor spheres initially cultured in serum-free medium with different concentrations of serum, focusing on the effect of microserum environment stimulat...
Ausführliche Beschreibung
Autor*in: |
Wang, Feiqing [verfasserIn] |
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E-Artikel |
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Englisch |
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2023 |
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Anmerkung: |
© The Author(s) 2023 |
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Übergeordnetes Werk: |
Enthalten in: Hormones and cancer - New York, NY [u.a.] : Springer, 2010, 14(2023), 1 vom: 28. Aug. |
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Übergeordnetes Werk: |
volume:14 ; year:2023 ; number:1 ; day:28 ; month:08 |
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DOI / URN: |
10.1007/s12672-023-00779-z |
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SPR052900541 |
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520 | |a Background 3D cancer stem cell (CSC) cultures are widely used as in vitro tumor models. In this study, we determined the effects of enriching HCT116 tumor spheres initially cultured in serum-free medium with different concentrations of serum, focusing on the effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells (CCSCs). Methods CCSCs were enriched in standard serum-free medium and serum-free medium with different concentrations of serum for 1 week. The expression of CSC-associated markers in CCSCs, and the presence and relative proportion of CSCs (CD133/CD44 cell sorting) were then determined to elucidate the effect of the microserum environment on the preservation of CSC-related features. Further, the tumorigenic capacity of CCSCs was evaluated in an immunodeficiency mouse model. Results Our data indicated that a significantly greater number of spheres with a greater size range and high viability without drastic alteration in biological and structural features, which maintained self‐renewal potential after sequential passages were formed after serum supplementation. Real-time analysis showed that both serum spheres and serum-free spheres displayed similar expression patterns for key stemness genes. Serum spheres showed higher expression of the CSC surface markers CD133 and CD44 than did CSCs spheres cultured in serum-free medium. Adherent cultures in complete medium could adapt to the serum-containing microenvironment faster and showed higher proliferation ability. The addition of serum induced EMT and promoted the migration and invasion of serum globular cells. Compared with serum-free cells and adherent cells, serum spheres showed higher tumor initiation ability. Conclusions Microserum environment stimulation could be an effective strategy for reliable enrichment of intact CCSCs, and a more efficient CSC enrichment method. | ||
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700 | 1 | |a Zhao, Jianing |4 aut | |
700 | 1 | |a Zhang, Chike |4 aut | |
700 | 1 | |a Yang, Bo |4 aut | |
700 | 1 | |a Tian, Tingting |4 aut | |
700 | 1 | |a Tian, Mengxian |4 aut | |
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700 | 1 | |a Zhu, Xiaodong |4 aut | |
700 | 1 | |a Su, Min |4 aut | |
700 | 1 | |a He, Zhixu |4 aut | |
700 | 1 | |a Liu, Yang |4 aut | |
700 | 1 | |a Tang, Dongxin |4 aut | |
700 | 1 | |a Li, Yanju |4 aut | |
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10.1007/s12672-023-00779-z doi (DE-627)SPR052900541 (SPR)s12672-023-00779-z-e DE-627 ger DE-627 rakwb eng Wang, Feiqing verfasserin aut Effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background 3D cancer stem cell (CSC) cultures are widely used as in vitro tumor models. In this study, we determined the effects of enriching HCT116 tumor spheres initially cultured in serum-free medium with different concentrations of serum, focusing on the effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells (CCSCs). Methods CCSCs were enriched in standard serum-free medium and serum-free medium with different concentrations of serum for 1 week. The expression of CSC-associated markers in CCSCs, and the presence and relative proportion of CSCs (CD133/CD44 cell sorting) were then determined to elucidate the effect of the microserum environment on the preservation of CSC-related features. Further, the tumorigenic capacity of CCSCs was evaluated in an immunodeficiency mouse model. Results Our data indicated that a significantly greater number of spheres with a greater size range and high viability without drastic alteration in biological and structural features, which maintained self‐renewal potential after sequential passages were formed after serum supplementation. Real-time analysis showed that both serum spheres and serum-free spheres displayed similar expression patterns for key stemness genes. Serum spheres showed higher expression of the CSC surface markers CD133 and CD44 than did CSCs spheres cultured in serum-free medium. Adherent cultures in complete medium could adapt to the serum-containing microenvironment faster and showed higher proliferation ability. The addition of serum induced EMT and promoted the migration and invasion of serum globular cells. Compared with serum-free cells and adherent cells, serum spheres showed higher tumor initiation ability. Conclusions Microserum environment stimulation could be an effective strategy for reliable enrichment of intact CCSCs, and a more efficient CSC enrichment method. Microserum environment (dpeaa)DE-He213 Colon cancer tumor cells (dpeaa)DE-He213 Cancer stem cells (dpeaa)DE-He213 Tumor sphere enrichment (dpeaa)DE-He213 Zhao, Jianing aut Zhang, Chike aut Yang, Bo aut Tian, Tingting aut Tian, Mengxian aut Meng, Na aut Xie, Wei aut Liu, Guangyang aut Zhu, Xiaodong aut Su, Min aut He, Zhixu aut Liu, Yang aut Tang, Dongxin aut Li, Yanju aut Enthalten in Hormones and cancer New York, NY [u.a.] : Springer, 2010 14(2023), 1 vom: 28. Aug. (DE-627)621547379 (DE-600)2543318-0 1868-8500 nnns volume:14 year:2023 number:1 day:28 month:08 https://dx.doi.org/10.1007/s12672-023-00779-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_120 AR 14 2023 1 28 08 |
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10.1007/s12672-023-00779-z doi (DE-627)SPR052900541 (SPR)s12672-023-00779-z-e DE-627 ger DE-627 rakwb eng Wang, Feiqing verfasserin aut Effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background 3D cancer stem cell (CSC) cultures are widely used as in vitro tumor models. In this study, we determined the effects of enriching HCT116 tumor spheres initially cultured in serum-free medium with different concentrations of serum, focusing on the effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells (CCSCs). Methods CCSCs were enriched in standard serum-free medium and serum-free medium with different concentrations of serum for 1 week. The expression of CSC-associated markers in CCSCs, and the presence and relative proportion of CSCs (CD133/CD44 cell sorting) were then determined to elucidate the effect of the microserum environment on the preservation of CSC-related features. Further, the tumorigenic capacity of CCSCs was evaluated in an immunodeficiency mouse model. Results Our data indicated that a significantly greater number of spheres with a greater size range and high viability without drastic alteration in biological and structural features, which maintained self‐renewal potential after sequential passages were formed after serum supplementation. Real-time analysis showed that both serum spheres and serum-free spheres displayed similar expression patterns for key stemness genes. Serum spheres showed higher expression of the CSC surface markers CD133 and CD44 than did CSCs spheres cultured in serum-free medium. Adherent cultures in complete medium could adapt to the serum-containing microenvironment faster and showed higher proliferation ability. The addition of serum induced EMT and promoted the migration and invasion of serum globular cells. Compared with serum-free cells and adherent cells, serum spheres showed higher tumor initiation ability. Conclusions Microserum environment stimulation could be an effective strategy for reliable enrichment of intact CCSCs, and a more efficient CSC enrichment method. Microserum environment (dpeaa)DE-He213 Colon cancer tumor cells (dpeaa)DE-He213 Cancer stem cells (dpeaa)DE-He213 Tumor sphere enrichment (dpeaa)DE-He213 Zhao, Jianing aut Zhang, Chike aut Yang, Bo aut Tian, Tingting aut Tian, Mengxian aut Meng, Na aut Xie, Wei aut Liu, Guangyang aut Zhu, Xiaodong aut Su, Min aut He, Zhixu aut Liu, Yang aut Tang, Dongxin aut Li, Yanju aut Enthalten in Hormones and cancer New York, NY [u.a.] : Springer, 2010 14(2023), 1 vom: 28. Aug. (DE-627)621547379 (DE-600)2543318-0 1868-8500 nnns volume:14 year:2023 number:1 day:28 month:08 https://dx.doi.org/10.1007/s12672-023-00779-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_120 AR 14 2023 1 28 08 |
allfields_unstemmed |
10.1007/s12672-023-00779-z doi (DE-627)SPR052900541 (SPR)s12672-023-00779-z-e DE-627 ger DE-627 rakwb eng Wang, Feiqing verfasserin aut Effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background 3D cancer stem cell (CSC) cultures are widely used as in vitro tumor models. In this study, we determined the effects of enriching HCT116 tumor spheres initially cultured in serum-free medium with different concentrations of serum, focusing on the effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells (CCSCs). Methods CCSCs were enriched in standard serum-free medium and serum-free medium with different concentrations of serum for 1 week. The expression of CSC-associated markers in CCSCs, and the presence and relative proportion of CSCs (CD133/CD44 cell sorting) were then determined to elucidate the effect of the microserum environment on the preservation of CSC-related features. Further, the tumorigenic capacity of CCSCs was evaluated in an immunodeficiency mouse model. Results Our data indicated that a significantly greater number of spheres with a greater size range and high viability without drastic alteration in biological and structural features, which maintained self‐renewal potential after sequential passages were formed after serum supplementation. Real-time analysis showed that both serum spheres and serum-free spheres displayed similar expression patterns for key stemness genes. Serum spheres showed higher expression of the CSC surface markers CD133 and CD44 than did CSCs spheres cultured in serum-free medium. Adherent cultures in complete medium could adapt to the serum-containing microenvironment faster and showed higher proliferation ability. The addition of serum induced EMT and promoted the migration and invasion of serum globular cells. Compared with serum-free cells and adherent cells, serum spheres showed higher tumor initiation ability. Conclusions Microserum environment stimulation could be an effective strategy for reliable enrichment of intact CCSCs, and a more efficient CSC enrichment method. Microserum environment (dpeaa)DE-He213 Colon cancer tumor cells (dpeaa)DE-He213 Cancer stem cells (dpeaa)DE-He213 Tumor sphere enrichment (dpeaa)DE-He213 Zhao, Jianing aut Zhang, Chike aut Yang, Bo aut Tian, Tingting aut Tian, Mengxian aut Meng, Na aut Xie, Wei aut Liu, Guangyang aut Zhu, Xiaodong aut Su, Min aut He, Zhixu aut Liu, Yang aut Tang, Dongxin aut Li, Yanju aut Enthalten in Hormones and cancer New York, NY [u.a.] : Springer, 2010 14(2023), 1 vom: 28. Aug. (DE-627)621547379 (DE-600)2543318-0 1868-8500 nnns volume:14 year:2023 number:1 day:28 month:08 https://dx.doi.org/10.1007/s12672-023-00779-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_120 AR 14 2023 1 28 08 |
allfieldsGer |
10.1007/s12672-023-00779-z doi (DE-627)SPR052900541 (SPR)s12672-023-00779-z-e DE-627 ger DE-627 rakwb eng Wang, Feiqing verfasserin aut Effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background 3D cancer stem cell (CSC) cultures are widely used as in vitro tumor models. In this study, we determined the effects of enriching HCT116 tumor spheres initially cultured in serum-free medium with different concentrations of serum, focusing on the effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells (CCSCs). Methods CCSCs were enriched in standard serum-free medium and serum-free medium with different concentrations of serum for 1 week. The expression of CSC-associated markers in CCSCs, and the presence and relative proportion of CSCs (CD133/CD44 cell sorting) were then determined to elucidate the effect of the microserum environment on the preservation of CSC-related features. Further, the tumorigenic capacity of CCSCs was evaluated in an immunodeficiency mouse model. Results Our data indicated that a significantly greater number of spheres with a greater size range and high viability without drastic alteration in biological and structural features, which maintained self‐renewal potential after sequential passages were formed after serum supplementation. Real-time analysis showed that both serum spheres and serum-free spheres displayed similar expression patterns for key stemness genes. Serum spheres showed higher expression of the CSC surface markers CD133 and CD44 than did CSCs spheres cultured in serum-free medium. Adherent cultures in complete medium could adapt to the serum-containing microenvironment faster and showed higher proliferation ability. The addition of serum induced EMT and promoted the migration and invasion of serum globular cells. Compared with serum-free cells and adherent cells, serum spheres showed higher tumor initiation ability. Conclusions Microserum environment stimulation could be an effective strategy for reliable enrichment of intact CCSCs, and a more efficient CSC enrichment method. Microserum environment (dpeaa)DE-He213 Colon cancer tumor cells (dpeaa)DE-He213 Cancer stem cells (dpeaa)DE-He213 Tumor sphere enrichment (dpeaa)DE-He213 Zhao, Jianing aut Zhang, Chike aut Yang, Bo aut Tian, Tingting aut Tian, Mengxian aut Meng, Na aut Xie, Wei aut Liu, Guangyang aut Zhu, Xiaodong aut Su, Min aut He, Zhixu aut Liu, Yang aut Tang, Dongxin aut Li, Yanju aut Enthalten in Hormones and cancer New York, NY [u.a.] : Springer, 2010 14(2023), 1 vom: 28. Aug. (DE-627)621547379 (DE-600)2543318-0 1868-8500 nnns volume:14 year:2023 number:1 day:28 month:08 https://dx.doi.org/10.1007/s12672-023-00779-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_120 AR 14 2023 1 28 08 |
allfieldsSound |
10.1007/s12672-023-00779-z doi (DE-627)SPR052900541 (SPR)s12672-023-00779-z-e DE-627 ger DE-627 rakwb eng Wang, Feiqing verfasserin aut Effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background 3D cancer stem cell (CSC) cultures are widely used as in vitro tumor models. In this study, we determined the effects of enriching HCT116 tumor spheres initially cultured in serum-free medium with different concentrations of serum, focusing on the effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells (CCSCs). Methods CCSCs were enriched in standard serum-free medium and serum-free medium with different concentrations of serum for 1 week. The expression of CSC-associated markers in CCSCs, and the presence and relative proportion of CSCs (CD133/CD44 cell sorting) were then determined to elucidate the effect of the microserum environment on the preservation of CSC-related features. Further, the tumorigenic capacity of CCSCs was evaluated in an immunodeficiency mouse model. Results Our data indicated that a significantly greater number of spheres with a greater size range and high viability without drastic alteration in biological and structural features, which maintained self‐renewal potential after sequential passages were formed after serum supplementation. Real-time analysis showed that both serum spheres and serum-free spheres displayed similar expression patterns for key stemness genes. Serum spheres showed higher expression of the CSC surface markers CD133 and CD44 than did CSCs spheres cultured in serum-free medium. Adherent cultures in complete medium could adapt to the serum-containing microenvironment faster and showed higher proliferation ability. The addition of serum induced EMT and promoted the migration and invasion of serum globular cells. Compared with serum-free cells and adherent cells, serum spheres showed higher tumor initiation ability. Conclusions Microserum environment stimulation could be an effective strategy for reliable enrichment of intact CCSCs, and a more efficient CSC enrichment method. Microserum environment (dpeaa)DE-He213 Colon cancer tumor cells (dpeaa)DE-He213 Cancer stem cells (dpeaa)DE-He213 Tumor sphere enrichment (dpeaa)DE-He213 Zhao, Jianing aut Zhang, Chike aut Yang, Bo aut Tian, Tingting aut Tian, Mengxian aut Meng, Na aut Xie, Wei aut Liu, Guangyang aut Zhu, Xiaodong aut Su, Min aut He, Zhixu aut Liu, Yang aut Tang, Dongxin aut Li, Yanju aut Enthalten in Hormones and cancer New York, NY [u.a.] : Springer, 2010 14(2023), 1 vom: 28. Aug. (DE-627)621547379 (DE-600)2543318-0 1868-8500 nnns volume:14 year:2023 number:1 day:28 month:08 https://dx.doi.org/10.1007/s12672-023-00779-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_120 AR 14 2023 1 28 08 |
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Enthalten in Hormones and cancer 14(2023), 1 vom: 28. Aug. volume:14 year:2023 number:1 day:28 month:08 |
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Microserum environment Colon cancer tumor cells Cancer stem cells Tumor sphere enrichment |
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Wang, Feiqing @@aut@@ Zhao, Jianing @@aut@@ Zhang, Chike @@aut@@ Yang, Bo @@aut@@ Tian, Tingting @@aut@@ Tian, Mengxian @@aut@@ Meng, Na @@aut@@ Xie, Wei @@aut@@ Liu, Guangyang @@aut@@ Zhu, Xiaodong @@aut@@ Su, Min @@aut@@ He, Zhixu @@aut@@ Liu, Yang @@aut@@ Tang, Dongxin @@aut@@ Li, Yanju @@aut@@ |
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In this study, we determined the effects of enriching HCT116 tumor spheres initially cultured in serum-free medium with different concentrations of serum, focusing on the effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells (CCSCs). Methods CCSCs were enriched in standard serum-free medium and serum-free medium with different concentrations of serum for 1 week. The expression of CSC-associated markers in CCSCs, and the presence and relative proportion of CSCs (CD133/CD44 cell sorting) were then determined to elucidate the effect of the microserum environment on the preservation of CSC-related features. Further, the tumorigenic capacity of CCSCs was evaluated in an immunodeficiency mouse model. Results Our data indicated that a significantly greater number of spheres with a greater size range and high viability without drastic alteration in biological and structural features, which maintained self‐renewal potential after sequential passages were formed after serum supplementation. Real-time analysis showed that both serum spheres and serum-free spheres displayed similar expression patterns for key stemness genes. Serum spheres showed higher expression of the CSC surface markers CD133 and CD44 than did CSCs spheres cultured in serum-free medium. Adherent cultures in complete medium could adapt to the serum-containing microenvironment faster and showed higher proliferation ability. The addition of serum induced EMT and promoted the migration and invasion of serum globular cells. Compared with serum-free cells and adherent cells, serum spheres showed higher tumor initiation ability. 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Wang, Feiqing misc Microserum environment misc Colon cancer tumor cells misc Cancer stem cells misc Tumor sphere enrichment Effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells |
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Effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells Microserum environment (dpeaa)DE-He213 Colon cancer tumor cells (dpeaa)DE-He213 Cancer stem cells (dpeaa)DE-He213 Tumor sphere enrichment (dpeaa)DE-He213 |
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Effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells |
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Effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells |
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Wang, Feiqing Zhao, Jianing Zhang, Chike Yang, Bo Tian, Tingting Tian, Mengxian Meng, Na Xie, Wei Liu, Guangyang Zhu, Xiaodong Su, Min He, Zhixu Liu, Yang Tang, Dongxin Li, Yanju |
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effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells |
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Effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells |
abstract |
Background 3D cancer stem cell (CSC) cultures are widely used as in vitro tumor models. In this study, we determined the effects of enriching HCT116 tumor spheres initially cultured in serum-free medium with different concentrations of serum, focusing on the effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells (CCSCs). Methods CCSCs were enriched in standard serum-free medium and serum-free medium with different concentrations of serum for 1 week. The expression of CSC-associated markers in CCSCs, and the presence and relative proportion of CSCs (CD133/CD44 cell sorting) were then determined to elucidate the effect of the microserum environment on the preservation of CSC-related features. Further, the tumorigenic capacity of CCSCs was evaluated in an immunodeficiency mouse model. Results Our data indicated that a significantly greater number of spheres with a greater size range and high viability without drastic alteration in biological and structural features, which maintained self‐renewal potential after sequential passages were formed after serum supplementation. Real-time analysis showed that both serum spheres and serum-free spheres displayed similar expression patterns for key stemness genes. Serum spheres showed higher expression of the CSC surface markers CD133 and CD44 than did CSCs spheres cultured in serum-free medium. Adherent cultures in complete medium could adapt to the serum-containing microenvironment faster and showed higher proliferation ability. The addition of serum induced EMT and promoted the migration and invasion of serum globular cells. Compared with serum-free cells and adherent cells, serum spheres showed higher tumor initiation ability. Conclusions Microserum environment stimulation could be an effective strategy for reliable enrichment of intact CCSCs, and a more efficient CSC enrichment method. © The Author(s) 2023 |
abstractGer |
Background 3D cancer stem cell (CSC) cultures are widely used as in vitro tumor models. In this study, we determined the effects of enriching HCT116 tumor spheres initially cultured in serum-free medium with different concentrations of serum, focusing on the effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells (CCSCs). Methods CCSCs were enriched in standard serum-free medium and serum-free medium with different concentrations of serum for 1 week. The expression of CSC-associated markers in CCSCs, and the presence and relative proportion of CSCs (CD133/CD44 cell sorting) were then determined to elucidate the effect of the microserum environment on the preservation of CSC-related features. Further, the tumorigenic capacity of CCSCs was evaluated in an immunodeficiency mouse model. Results Our data indicated that a significantly greater number of spheres with a greater size range and high viability without drastic alteration in biological and structural features, which maintained self‐renewal potential after sequential passages were formed after serum supplementation. Real-time analysis showed that both serum spheres and serum-free spheres displayed similar expression patterns for key stemness genes. Serum spheres showed higher expression of the CSC surface markers CD133 and CD44 than did CSCs spheres cultured in serum-free medium. Adherent cultures in complete medium could adapt to the serum-containing microenvironment faster and showed higher proliferation ability. The addition of serum induced EMT and promoted the migration and invasion of serum globular cells. Compared with serum-free cells and adherent cells, serum spheres showed higher tumor initiation ability. Conclusions Microserum environment stimulation could be an effective strategy for reliable enrichment of intact CCSCs, and a more efficient CSC enrichment method. © The Author(s) 2023 |
abstract_unstemmed |
Background 3D cancer stem cell (CSC) cultures are widely used as in vitro tumor models. In this study, we determined the effects of enriching HCT116 tumor spheres initially cultured in serum-free medium with different concentrations of serum, focusing on the effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells (CCSCs). Methods CCSCs were enriched in standard serum-free medium and serum-free medium with different concentrations of serum for 1 week. The expression of CSC-associated markers in CCSCs, and the presence and relative proportion of CSCs (CD133/CD44 cell sorting) were then determined to elucidate the effect of the microserum environment on the preservation of CSC-related features. Further, the tumorigenic capacity of CCSCs was evaluated in an immunodeficiency mouse model. Results Our data indicated that a significantly greater number of spheres with a greater size range and high viability without drastic alteration in biological and structural features, which maintained self‐renewal potential after sequential passages were formed after serum supplementation. Real-time analysis showed that both serum spheres and serum-free spheres displayed similar expression patterns for key stemness genes. Serum spheres showed higher expression of the CSC surface markers CD133 and CD44 than did CSCs spheres cultured in serum-free medium. Adherent cultures in complete medium could adapt to the serum-containing microenvironment faster and showed higher proliferation ability. The addition of serum induced EMT and promoted the migration and invasion of serum globular cells. Compared with serum-free cells and adherent cells, serum spheres showed higher tumor initiation ability. Conclusions Microserum environment stimulation could be an effective strategy for reliable enrichment of intact CCSCs, and a more efficient CSC enrichment method. © The Author(s) 2023 |
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Effect of microserum environment stimulation on extraction and biological function of colorectal cancer stem cells |
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https://dx.doi.org/10.1007/s12672-023-00779-z |
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Zhao, Jianing Zhang, Chike Yang, Bo Tian, Tingting Tian, Mengxian Meng, Na Xie, Wei Liu, Guangyang Zhu, Xiaodong Su, Min He, Zhixu Liu, Yang Tang, Dongxin Li, Yanju |
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Zhao, Jianing Zhang, Chike Yang, Bo Tian, Tingting Tian, Mengxian Meng, Na Xie, Wei Liu, Guangyang Zhu, Xiaodong Su, Min He, Zhixu Liu, Yang Tang, Dongxin Li, Yanju |
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